CN1869009A - Method of extracting carbamyl ornithine from watermelon rind - Google Patents

Abstract

A process for extracting citrulline and other nutrients from the peel of water melon is disclosed.

Description

A kind of method of from Watermelon rind, extracting citrulline

The present invention relates to the comprehensive utilization of watermelon, particularly relate to the method for from Watermelon rind, extracting citrulline.

Up to now, rest on watermelon (juice) beverage and watermelon can aspect for the comprehensive utilization of watermelon always, and the patent application of this class early since 89 years along continuous to 2002, application has ten several pieces, but does not obtain very big breakthrough.Country once invested this type of producer with relevant area, but all ended in failure.Watermelon is high sugared, nutritious fruit, and breed bacteria is difficult for preserving very much especially easily, and therefore present watermelon (juice) beverage and watermelon can still do not obtain industrialization.

The watermelon whole body is precious, and the food medicine has both.Skin, flesh, seed, shell, leaf, rattan all available it.Say in the Compendium of Material Medica: watermelon " disappear tired quenching the thirst, separate hot summer weather, treat larynx numbness, the wide middle therapeutic method to keep the adverse qi flowing downward, Li Xiaoshui controls bloody flux, relieving alcoholism." also record in " edible book on Chinese herbal medicine ": " and watermelon detoxifcation cold in nature, have the nature Baihu Tang number ".Modern study more shows and contains materials such as protein, glucose, citrulline, L-glutamic acid, Lyeopene, Vitamin A, B, C, mineral substance in the watermelon pulp.Yet these functions of watermelon are not by utilization that people are familiar with for a long time, and behind the edible melon pulp of people, melon skin, melon seeds abandon everywhere, have a strong impact on and destroying environment.

The present invention utilizes the abundant watermelon resource in area, breaks through in the past traditional watermelon comprehensive utilization idea, utilizes the biological extraction technology to extract the nutritive substance with certain physiology and nourishing function from the watermelon each several part.Be rich in citrulline in the Watermelon rind, citrulline can make human body produce oxynitride, and this oxynitride is a kind of very important material to the male sex's sexuality.Different with raising sexuality class medicine is that citrulline can absorb in the blood fully, more easily produces oxynitride, thereby reaches the effect that makes erection.It is a kind of natural product, without any side effect, can also strengthen man's physical appearance simultaneously comprehensively.

The invention provides a kind of method of extracting citrulline from watermelon, its step is as follows:

(1) watermelon is cleaned, removed epidermis and pulp, obtain the melon kingfisher;

(2) press juice, filtration to obtain the emerald green juice of melon emerald green pulverizing of melon;

(3) at concentrated juice below 80 ℃, regulate below the pH value to 5, decolour after leaving standstill and handle and filter, obtain the achromaticity and clarification filtered liquid;

(4) above-mentioned filtered liquid is passed through ion exchange resin column, until saturated;

(5) resolve wash-out with the ammoniacal liquor gradient, collect the part desorbed solution of PH3-12;

(6) ammonia excretion of gained desorbed solution and decolouring;

(7) destainer concentrates, crystallization, filtration, drying obtain the citrulline crystal.

The citrulline that obtains can be made into tablet and capsule, except that being used to improve the male's sexual, also can stimulate circulation, protect cardiovascular; As healthcare products anti-ageing, that improve immunizing power; Also can be used as women's cosmetics, have the wrinkle resistant anti-ageing effect of spot of going of skin care.

The green epidermis of watermelon melon skin outermost layer can extract natural pigment---chlorophyll by the method for solvent method or supercritical extraction, remaining watermelon robust fibre; The melon skin kingfisher of removing shagreen is after extracting citrulline, and remaining part can further be developed leaf protein and scleroproein, particularly extracts the beverage that the distilled water that obtains behind the citrulline can be made into rich in mineral substances and has watermelon delicate fragrance, but prolonged preservation.

Watermelon pulp can extract the lycopene with strong anti-oxidation, anti-cancer function by the method for solvent method or supercritical extraction, and the surplus materials that obtains can be made into pulp and above-mentioned rich in mineral substances and has the beverage of watermelon delicate fragrance.

Melon seeds benevolence can be extracted by the method for supercritical extraction has the Semen Benincasae oil that enriches unsaturated acid and essential amino acid and VE, can extract the melon seeds protamine in the residue.

Embodiment 1

4970 gram watermelons are scraped off green epidermis, dig out pulp, obtain 1320 gram melon skin kingfishers, pulverize the back and press juice to obtain 1120 gram juice, be concentrated into 450ml, transfer to pH value below 5 with formic acid one hydrochloric acid, behind the standing demix, add 1% activated carbon decolorizing 30 minutes, place 4 hours after-filtration, filtrate achromaticity and clarification under the room temperature.

This filtrate with 0.5～1.0N ammoniacal liquor gradient elution, is collected the PH3-12 desorbed solution through macroporous resin exchange absorption and behind water balance.

Desorbed solution is through concentrating, after crystallization and the drying, obtaining citrulline crystal 4 .5 gram.

Claims (1)

1, a kind of method of extracting citrulline from Watermelon rind may further comprise the steps:

(1) watermelon is cleaned, removed epidermis and pulp, obtain the melon kingfisher;

(2) press juice, filtration to obtain the emerald green juice of melon emerald green pulverizing of melon;

(3) at concentrated juice below 80 ℃, regulate below the pH value to 5, decolour after leaving standstill and handle and filter, obtain the achromaticity and clarification filtered liquid;

(4) above-mentioned filtered liquid is passed through ion exchange resin column, until saturated;

(5) resolve wash-out with the ammoniacal liquor gradient, collect the part desorbed solution of PH3-12;

(6) ammonia excretion of gained desorbed solution and decolouring;

(7) destainer concentrates, crystallization, filtration, drying obtain the citrulline crystal.