CN1840696A - Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice - Google Patents

Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice Download PDF

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Publication number
CN1840696A
CN1840696A CN 200610004058 CN200610004058A CN1840696A CN 1840696 A CN1840696 A CN 1840696A CN 200610004058 CN200610004058 CN 200610004058 CN 200610004058 A CN200610004058 A CN 200610004058A CN 1840696 A CN1840696 A CN 1840696A
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China
Prior art keywords
rice
disease
molecule marker
gene
resistant gene
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CN100410390C (en
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朱旭东
邓一文
陈红旗
何祖华
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China Rice Research Institute
Shanghai Institutes for Biological Sciences SIBS of CAS
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INST OF PLANT PHYSIOECOLOGY SH
China National Rice Research Institute
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Abstract

The related rice gene mark for broad spectrum anti rice blast includes mark length 10-30 nucleotides with sequence same as the sequence of 1st-70798th in SEQ ID No:1. Wherein, based on our rice resource and natural or induced diseases, it applies different rice blast plants with the domestic as main and foreign as assist to screen the 'Gumei 4th', uses molecular mark technique to locate gene and obtain Pi-gm(t) gene with chain mark, and has great application value in breeding.

Description

The molecule of broad-spectrum rice-blast resistant gene of paddy rice is differentiated and transfer technology
Technical field
The invention belongs to and utilize molecular biology method to differentiate the technical field of broad-spectrum rice-blast resistant gene of paddy rice.It relates to the nucleotide sequence molecule marker that can be used for differentiating broad-spectrum rice-blast resistant gene of paddy rice and with disease-resistant gene and disease-resistant variety thereof that this molecule marker was obtained.
Background technology
Paddy rice is a kind of important cash crop, is related to national economy; Rice blast is one of the most common in China's Rice Production, destructive maximum fungal disease, also is that global paddy rice mainly produces rice state the great disease that this very easily causes the serious underproduction of paddy rice after being ill takes place.The harm of 1975～nineteen ninety because of rice blast causes that global paddy is with a toll of 1.57 hundred million tons.According to the incomplete statistics of plant protection master station of the Ministry of Agriculture, the year of 1987～1997 years 8 provinces such as serious Zhejiang, Guangdong, Jiangxi, river, Hunan, Hubei Province, cloud, the Liao Dynasty of causing harm area taking place reaches more than 3,800,000 hectares, the annual output loss is paced up and down at 20～700,000 tons.
Have reasons such as genetic background and population variation complexity owing to rice blast, cause breeding for disease resistance to be difficult to adapt to the quick variation of new population, and the kind that causes breeding only just loses the sick utility value of its control after planting 3～5 years in the production, transfer to rely on the expansion of sterilization class agent for preventing and controlling rice blast and spread, cause the vicious cycle of ecological environmental pollutions such as soil and water source then.Traditional breeding for disease resistance depends on resistance and identifies and the plant Phenotypic Selection, requires the time that has rich experience and reach several years even more than ten years.Owing to be subjected to the restriction of rice blast onset condition, and lack the effective method for tracing of enantiopathy gene, be difficult to select the permanent disease-resistant kind that has a plurality of disease-resistant genes simultaneously in the conventional breeding.
Paddy rice is accompanied by rice blast in the common process that develops, and has formed different disease-resistant mechanism and abundant disease-resistant gene.Cloned some blast resistant genes such as Pi-ta and Pi-b etc. at present from paddy rice, but these are not had broad spectrum by cloned genes to rice blast resistance, the germplasm that carries this genoid loses the utility value of variety resistance improvement already in China.From abundant domestic and international seed rice resource, excavate the new germplasm that wide spectrum, lasting blast resisting are arranged, utilize the high molecule marker of modern molecular engineering exploitation detection efficiency, cultivate wide spectrum, lasting blast resisting kind and be improve disease-resistant variety working life, reduce production costs, reduce environmental pollution, be the effective way of development sustainable agriculture.
Summary of the invention
The present invention utilize modern molecular marking technique analyze and near disease-resistant gene or gene itself effectively the selected nucleotide sequence molecule marker be that the molecule of broad-spectrum rice-blast resistant gene of paddy rice is differentiated, in shifting the disease-resistant gene process, be used, improve efficiency of selection and effect; The present invention is based on the basis of excavating Chinese seed rice resource " No. 4, Gu Mei ", extensively screens the representative bacterial strain in China different ecological rice district and carries out artificial inoculation.
Above-mentioned technical problem of the present invention is implemented by the following technical programs: a kind of molecule marker of broad-spectrum rice-blast resistant gene of paddy rice, it is characterized in that described molecule marker length is 10～30 Nucleotide, wherein the sequence shown in the 1-70798 position is identical among the sequence of molecule marker and the SEQ ID No:1.
As preferably: described molecule marker length is 15～25 Nucleotide.
As preferably: described molecule marker is selected from: SEQ ID No:2,3,4,5,6,7.
The new disease-resistant gene or the site that also provide the kind of developing according to the marker assisted selection of deriving of above-mentioned molecule marker design, the mark of deriving that designs according to above-mentioned molecule marker to obtain in the present invention with disease-resistant site; And the disease-resistant variety of identifying according to the labeled derivative of deriving of above-mentioned molecule marker design.
" No. 4, Gu Mei " is the local variety that China excavates the sixties in last century, shows anti-pest lasting, wide spectrum through lesion plantation for many years.With the joint study of France in, be used to the most of resistant variety used from country variant and geographic 29 current fields of strong virulence inoculation China and known wide spectrum anti-pest gene Pi-1 and anti-source that some are famous, as Tetep, Moroberekan, the anti-pest sex expression that its two-story valley plum is No. 4 is outstanding especially, it can resist infecting of all bacterial strains, significantly better than some famous anti-sources.
The present invention is on the basis of excavating Chinese seed rice resource " No. 4, Gu Mei ", extensively screening China representative bacterial strain artificial inoculation in different ecological rice district, confirm that " No. 4, Gu Mei " has broad spectrum resistance, utilize modern molecular marking technique to analyze near disease-resistant gene or the effective selective marker of gene itself, in shifting the disease-resistant gene process, be used, improve efficiency of selection and effect.
Blast resistant gene Pi-gm (t) has different physiological strains generation broad spectrum resistances.To the paddy plum carry out for No. 4 the genetic analysis result show its to the resistance of a plurality of bacterial strains from same site Pi-gm (t), and with Pi-2 and Pi-9 equipotential.Method by map based cloning is positioned at the 6th karyomit(e) to this disease-resistant site Pi-gm (t) or Pi-2 or Pi-9 near kinetochore about 70kb region S EQ ID No:1, promptly should there be a gene cluster that contains 6 disease-resistant genes in the zone, the present invention will be the molecule marker that utilizes the disease-resistant site of the 70798b of this SEQ ID No:1, and disease-resistant gene Pi-gm (t) or Pi-2 or Pi-9 are used for disease-resistant molecular breeding.
The present invention has the following advantages:
The present invention is a seed rice resource of utilizing China own, for many years, multiple spot lesion nature or induce on the basis of morbidity, is to assist by artificial inoculation based on domestic rice blast fungus different strains, external bacterial strain, filters out " No. 4, Gu Mei " from the rice germplasm resource; (2) utilize molecular marking technique, the segregating population to the paddy plum makes up with susceptible variety hybridization for No. 4 carries out the location of gene, and has obtained Pi-gm (t) gene and chain with it molecule marker thereof; (3) Pi-gm (t) gene and chain with it molecule marker thereof can improve breeding efficiency in the rice anti-rice blast breeding, prolong the utility value that kind is being produced.
Description of drawings
The electrophorogram of PCR product after restriction endonuclease EcoR72 cuts of accompanying drawing 1 mark C0428 anti-(R), sense (S) individual DNA in the BC2F2 seed selection that with susceptible parent Maratelli is recurrent parent and paddy plum 4 structures.
Accompanying drawing 2 mark C5483 are anti-in the BC2F2 seed selection that with susceptible parent Maratelli is recurrent parent and paddy plum 4 structures, the electrophorogram of PCR product after restriction endonuclease EcoR I cuts of the individual DNA of sense.
Accompanying drawing 3 mark S29742 are anti-in the seed selection in the selfing breeding material of recombinating with two extensive F6 that select 22 (SHX22) and paddy plum 4 to make up, the PCR product electrophorogram of the individual DNA of sense.
Embodiment
Below be specific embodiments of the invention and in conjunction with the accompanying drawings, technical scheme of the present invention is done the description in a nearly step, but the present invention be not limited to following embodiment
Below all examples, except that specializing, all utilize conventional molecular biology method: 1) JosephSambrook, David W.Russell eds.Molecular Cloning:A Laboratory Manual.Cold Spring Harbor Laboratory Press, 2001; 2) Carl W.Dieffenbach andGabriela S.Devksler eds.PCR Primer:A Laboratory Manual.Cold SpringHarbor Laboratory Press, 1995.Below patent is done more detailed example explanation.
Embodiment 1
The acquisition of " No. 4, Gu Mei "
The seed rice resource that China is own for many years, multiple spot lesion nature or induce on the basis of morbidity, is to assist by artificial inoculation based on domestic rice blast fungus different strains, external bacterial strain, filters out " No. 4, Gu Mei " from the rice germplasm resource;
(2) to paddy plum No. 4 and the segregating population that susceptible variety hybridization makes up, carry out the location of gene, near about 70kb zone, kinetochore, its sequence is shown in SEQ ID No:1 at the 6th karyomit(e);
SEQ ID No:1 total length is 70798bp.
Embodiment 2
Disease-resistant gene Pi-gm (t) that the paddy plum is No. 4 or Pi-2 or Pi-9 have carried out Fine Mapping at 70kb region S EQ ID No:1, have identified 6 molecule marker SEQ ID No:2,3,4,5,6,7 that lay respectively at this disease-resistant gene bunch SEQ ID No:1 two ends and 6 PCR-based of intermediary.
Table 1: the essential information of each molecule marker
Molecule marker Sequence Numbering
SEQ ID No：2 5’-GGGAGGAGGAATGGTAGGAA-3’(1- 19) C5483-R
SEQ ID No：3 5’-CCCAAGACAAGCAGCCTAA-3’(450- 468) C5483-F
SEQ ID No：4 5’-CAGTGAAACGAACGCTATG-3’(13576 -13594) S29742-F
SEQ ID No：5 5’-CAACATCATCCCTTCCTATT-3’(14010 -14029) S29742-R
SEQ ID No：6 5’-AAGGTTCTCGTGGTTTCA-3’(55062 -55079) C0428-F
SEQ ID No：7 5’-CTGCTATAAACAATGGGGA-3’(55725 -55743) C0428-R
Wherein, SEQ ID No:2,3,6,7 needs enzyme to cut.
Embodiment 3
Utilize the kind with disease-resistant site of the marker assisted selection exploitation of deriving of molecule marker design; Can whether exist in the disease-resistant site of Rapid identification as Fig. 1, Fig. 2, these molecule markers that utilize shown in Figure 3, can proceed the improvement of objective trait for the plant of disease-resistant site existence, so just can shortening the breeding cycle, compare with present conventional breeding, accelerate breeding process, saved great amount of manpower and material resources, financial resources.
Embodiment 4
New disease-resistant gene or site that the labeled derivative mark of deriving of molecule marker design obtains by other disease-resistant varieties that utilize these marks to identify, thereby can obtain new Pi-gm (t) allelotrope faster.
Embodiment 5
The disease-resistant variety that the labeled derivative of deriving of molecule marker design is identified utilizes these marks Rapid identification to contain disease-resistant gene Pi-gm (t) or Pi-2 or Pi-9 and allelic disease-resistant variety thereof.
Specific embodiment described in the present invention only is that the present invention's spirit is illustrated.The technician of the technical field of the invention can make various modifications or replenishes or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Although the present invention has been made detailed explanation and has quoted some specific exampless as proof, to those skilled in the art, only otherwise leave that the spirit and scope of the present invention can be done various variations or correction is obvious.
Sequence table
＜110〉China Paddy Rice Inst, Shanghai plant physiology ecological Studies institute of the Chinese Academy of Sciences
＜120〉broad-spectrum rice-blast resistant gene of paddy rice molecule authentication technique
<130>0571327
<160>21
<170>patentIn version3.1
<210>1
<211>70798
<212>DNA
＜213〉paddy rice
<400>1
gggaggaggaatggtaggaacactaaaaaccgaaaacttttagtggaattaaaagttaccttcgaagctgatggagat 78
cggtctgaccggcttgattgccctagtttgaccgcgccttgttgccaatctaaccggtgtagatcgaggtctgaccgccc 158
ttcgcgccatcgacgcttgttgccgccatataactgccggtgtgaccgagcagttgcctctggtctgatcgctggtgatc 238
tgtcggtgtgatcgctgcatctgagcaaaacacaaattaaaagatgtcttgaaagttcgtagattgaattttattgctta 318
tattcgtgttacaaagtacaacaacaacacttctctcacagaattcgactaaactcgaaaccttaacttttctctaaatt 398
aaactctcccaaaatcgatataaggatatctcaactccctctctatttatacccaagacaagcagcctaaagtcacgaat 478
ctaatttgtacaagaagttctaattcactagaaaaccttcccgtacaagaaacaaacttatctcatcataattcgaattt 558
caatcctcccaaatttagactccttccaagtttgactccgcttttcatacgcacacaatctcccattgaaacggtcaatt 638
aggcctaaggggggggtgaatgggctaatttaaaaacttaagtaaatgcagaagcatggtttttcagaaattctgaaaat 718
gctttacgaaaattctgaaaatcacataatatgcacaagtgaaagtaaattctagatctagctacatacaacaatgaaat 798
acaagcacaaacaacaactagacttataacggtacaaacaagcaaagctagaggagggagagaggtatatcaccgaggtt 878
gttgcaataagagttgttcctgaagtttgaatccttaaggggattatactttccgttgaggagctcacaacgagccgggt 958
ctttgctaacccttttctcaaggggttgcccaaagcactcctccttccactagtggtatctaggggtgaaaacgatcgga 1038
atcgttagcatctcttcggaaacgatgctcgatcggccggttagtcctcacatatactcaaacgaaaatgttagtcctct 1128
ctaatcatattgttattaatcactaaaatcattaggggcctagatgcactttcacccattttatgcgacaaagaatcatc 1198
ataaaacaatgtgcattgttctttagactaagcatcccgtacgatattctgattgtccagacatcatcttctcccaagtt 1278
gactctcgatccatcaccgacaacgctctcctgaggcatcaagacacacttacacaggaatcaaaacaagaaaccatatc 1358
cgagcacaagttttttcctaacttgactcgacattagcaaacaacaatattacacacatatagaaataatctagaagtca 1438
taattatgagataaacatgagtatccaaataaacaactcaaaaccgaccctaatcaggatccagccagtctgcccgcaca 1518
taccacaccggtctgaccggccttaccggtacagtctgatcgacttcacacaaaaaaaacacttcaccaataatcaccaa 1598
atactaaattaattatctatcatgccaattattcatcacaaattaataataaaagcacactttgatttcacacaattagt 1678
taactgacagtcatatcgatatatagccaaatatcggcaataattgtacaagctccctacatctattctcttaggttctc 1758
ctagatcgatcacactggtctactccttccatcccataaaatacaaacgttatcccaaatgtgagacaccataatactgc 1838
aaatctggagaggcgtgattaataatttctcatcagccataggggtaataataatccaaggctgtgtttagttccacgta 1918
aaaattgaaagtttaaagaaattggaacgatgtgacgaaaaagttggaagtttgtttgtgtaaaaaagttcaatgtgacg 1998
aaaaagttaaatgtttgaagaaaaaagtttgactaaacagggccaagtcatggtgatggtaagtcagcccctaagctttc 2078
actttgacctattctactctcacactgacgttgacattgtatcacagggcaccacccattccctctccttaatttttgtt 2158
tgactatagtaaccatatcaatcaaataggtgtgaaagctaacctagattatttctgatcacactgatcacattctcact 2238
agctattctacactttctaccttcacataggacgacaagtgtaccaacctactgataaattttcaagcctcatttttctt 2318
ataagttatggccaaaattaaaattttaaaaattagttttaaattattttgatgttgtttcatcacctttttctagcttt 2398
agctaaaatgatataatgatagagaaaaataaaagtcataacctaaaaataatttttagctcgttttttatagcttatta 2478
gcagcagactattcaactcgaccctccgtgttttctcatataagcccataccatggctacgtttgaaactacaactctac 2558
aagttctcatattttattttcttatttttcacaagtacgtttttgaaactgtaaaacgacgtgtgtttctaaaattttct 2638
atagaaaaattactttaaaacatcatataaatttattttaatttttttaactaatactacctctgtcctagaaagactgc 2718
agttttgcactattcatatccaacgtttgaccgtccgttttatttgaaagttttttataattagtatttttattgatatt 2798
aaatgataaattatgaataatattttatgtgtgactattttttaatttttttaataaattttcaaacaagacggacgtgg 2878
ttagtatttttattgttattagatgataaattatgaataatactttatgtgtgactattttttttaaatttgttttataa 2958
attttcaaataagacagacagacaaacgttggacaccaaaattcataactacagtcaaaatgggacggaggtagtaatta 3038
attaaccttttttaagaagaaatgagaagttcgaacacaacccgcacacccaaaccaagggggtgtttagattgaggggt 3118
gtaaagttttggcgtgtcacatcggatattatatatgatgttgtatagggtgttcggacactaataaaaaaaatcgactt 3198
attaagcctaattaatccgtcattagcatatgtttactgtagcacaacattatcaaatcatgtagcaattaggcttaaaa 3278
gattcgtctcgcaaattagtaacaatctatataattagctattttttagcctatatttaatatattatacagatgtccaa 3358
acgttcgacacgacgggatctaaaattttcgggtgggctcatcgaaagcaaacaaaacgaacccccgcacacgaacggtc 3438
acacgctgatcttttctccaaacccaaaaaagaaaagaaaatcagaaaaagtcccgccactacctctactactgccggcc 3518
ctacccccacagattccaggcgccagctaagcacacaccgacgtgcacccctcccctcccgcatgcgtttccactctgtc 3598
tccgctccacacccgagcccggtcaaacccaaccccgccgcgccgtcactccgcacccgactcactggcccccaccaccg 3678
accaccgccgctgccctgtgggccccgcccccacccgccgacgcgtgggcccccacacgccgccggccgcggcgggtcac 3758
atgcgcgtcgtccaaactccgaggcgcgcacgaaaaagagagagacaaaaaaaaaagaaaagagaaattctttttaggcg 3838
ctctaggtataaaaatctactcctactcatcaactacttttccacttcgattcctctccccttccccctcctcttcctcc 3918
ctctcctctcctctcctctccgccgctgccgctgctgctgcgtgctcctctcatccccgtctcttccccctccgcgcgcg 3998
ccgcccactcgctgggaggaggaggaagaggagaccttccccggaattcgtgctcgccggatcgggctcgccgcaatcca 4078
tgtcggtgagtggtgctgttgatgtgtttcttttttctactgattttagagttggtgattggttgcgctgtctagatcga 4158
gctgacccgcctgcgtgttggattgtattgattagaggaagggaggctgattagcacgaggtgtggggaaaaattagttg 4238
taaaaaaaaaattggaggggttaatagatgggcgtttgttaagtgacgtaaggcgaaagtgatgttatgctgttctgggt 4318
tagtaaggttcttggcaatcagttttggtcaaatcttactggttcataggtgttttggtcgaatttcagcttcagatgaa 4398
gtttgtccctcgtttccaggattgttggcggtcagttcttcatcgttaggcttttaattggttgaacaggaaattggggt 4478
catgatagaatgcgaagtttctgaaaacataagtagagaacaaaagtaggagaattttgtcaattaggtacgatggaagg 4558
tcaccgtcagcttgttcaactactgttaggttcaaactttgatcgtgcttgagatttctttgctaaaataaggggatatg 4638
tggcaaagggagaataaaccatgatatgatttttgttactgactatagtggctgtaaatgagaacttgtggaatcacaga 4718
aagttccaaaaaacaatgcagaaattgtatccttttttgtgctttgcctagtccattttctgacttctgagatgaggtcc 4798
ccgcatcacatagaactgcaattgcaattcaatgcattcatgcaacaaacagacagactactgtcctataacatgagtta 4878
tgacttacgagtagccttgcatattgtacatgcataagccaaagctgcttgtttattgtaaaccagatgctctgtaccat 4958
aaaaaccataatccattaagttttcttgtttactttgttgaacattatactagatacctatggtgttgactgttacataa 5038
cattctgttcaccatttggcaattttccccccttagtgacattcattcttttgagatttcagtcatgccttccacatgac 5118
aaaatgttccattcaactatgatgtttggaattgggaaacggtacacatacttaatgtttcttttactaattctgattga 5198
aagaatgcaacacatcttttaatccacatgtacatacatcaatcaatgctttggagagagtgcatcatggatcaatcata 5278
ttgatggtttattagtaaacatttcacagcaattatttgtgcacatggccctcatacaatttttgttgggcattcaaccc 5358
ttgttttgaggtgttaaataaattattttttattatgttgtcaaattgcctgacctttgtattggtggttgggcatctag 5438
ctgtgcactgtcaataactcctggcggtgctcctctttttcctggctgttgaacttccaatctgaccattcattatgttc 5518
atatcttgtacacatgtggtgagatgtcaattgctgtccattgtatgttactatattttacttagcattgttcaactgta 5598
atagacagtgatatatcatacatgtcaaatgaaaccaacacagacaaagcatatgctgtgttgtcatcttcttatttctt 5678
gtactgttctgttctgtcttgaaggttagctgtgttagtcatggcccactatggattctcaaaaggcatcctttatgttc 5758
attagtttcatctttttctattttccaattttattagatggctatactcgactttgttcacacataatctctaattatca 5838
atcgtctggtcatgcttggcaggcctcaatcaaatgccagcccacccacgggaaatgggccaggattgaggcctgctgaa 5918
caaggtgtagaggtagatacaccctttaatgtggatcatggttcctttctaggaggaagcaggtcgtcccttgcccaagt 5998
ttggtgaatgggatgtcaacgacccagcgtccgctgatggattcacagtgatattcaacaaagccagagatgagaaaaag 6078
ggtgggaatgggcaagatactgattcaccctgcaaagaaactaggactgagagggtggaatcatatgcccccaagacaaa 6158
ctcggtatgtctattacattactcacgcttattataatatcagaatttcaatttctccctgattaatataacgatattcc 6238
ttttctcttgaactacataactagctagatgtcattaatctcattgttttcatgtgcagaagaaatggttttgctgcgtg 6318
acatccagtcctacacaatcttgatgaaaacgagttccatgggttgcaaaattactatcctttaattttgctatatacat 6398
actatccataagaccttttagagatgcccagactctactgcggtgctagattgggcatctcttaaaactttgaggtgtgt 6478
gtatgtatgtgtgagggttatcagatgcacattcggataaatgaacttctgattgtaattctagccttccgtcctgtgac 6558
attctgtgatgtagtcgttattcagtgatataatgaatctcacctattgatataactgcacactgtttctcttgctttcc 6638
gaggaaacaaacactgttttaccaaagttggctgtgctccaaaagaggattaaattccatatgattctcatattgcaatt 6718
gcaattttgattctctcacctactaatatgcgattttggaattttcctcacgtgccaaatttgcatattgcaattttgat 6798
aaaggatacaagcacagtatagctcaatctccttggcgatggaagttttcttaatattgtaggcaccacatttctagctg 6878
ataatgcaactatcgtggagatcataaagaggaggaattttgtggaagaaggacctggaggttgggagtcttcggcctct 6958
actcagtcaaatacaagaaaatattcctcaaaacttaatgcatgtgatgtagattcaaaaagaggtgaatcaactagctg 7038
ataagttagcaaaggatgtgagatcgcgacagctaaatccttcaggtttatgattgtcaaaatatcttgagtcttgctca 7118
tcctagaaacctttgttatgttagagatttgaagaaccatttcagagcaacgaattgtattacaactttacaagtcatgt 7198
gctctgtttctgaaatggaaataaaactgatgagttctaaaaaaaaaaagatccatacattcatgcctgtacacggacag 7278
caaatctacatgtcagagtggatatcgccaaattggtttcagaatttcatcattgtgctcatgcccaatattttttaatt 7358
ttcggtgaggaacagatgagcatctttcgtgctagctgcagactcgggtgcactaggaatttcactggtttatttcacac 7438
gatttatttcagttccactgaaatttgtttggaattcatttggttcgagaagaggcgtgagatacgagactttgggagag 7518
ggaaagggcagttcaatacctgatacttttgctcacttggagcttgaccggagagaggggagggagagtagtgagggggg 7598
agcagtcgatggcggcggagacggtggtgagcatggcgatgtcggtgctgggcagcgccgtcgggaaggccgcctccgcc 7678
gccgccgacgaggccaccctcctgctcggcatccagaaggagatctggtatgtgtgcgcacgcttctgtaactccatacc 7758
acatcggtgactctccatggcccctccgcggcggcgtgtcggcgacctcggcttccatggccgccgccgccgccgcctca 7838
cagccctttttggtcttttggtgattccaacggattcaggatcaaaaatgaaacaatccggagagcctattcacgcttcg 7918
catttcactaccccaaacttgtggattgtgaccatgcaaaccaaaattccttcagtttcatactttcatatgctcaaatt 7998
tccgttgttttcatagaaacaaggttatttatcccacttacatcttgcaaaaaccaaagtacagttactcttatcgattt 8078
tcatctatacacatgctagacgaatgcattgtagtccaattcaaatttctgatatattagttactaaccaagatgctccc 8158
atgccatgagcgcaagttccattgacaaggaaaaacacttttttatgatttgagcaagttccacgctggcgtcctcgttg 8238
gcacaccacgtaagctaaagtggcaaaaaaaaaagaatttttctctctacagtgagtcagtgacaaaaacttaagtgcaa 8318
aattaaaatgggtatttggataatcgccaaatctaaaagtggcaaatagttaaattccccttttatatttctctcttcag 8398
tggcaaaaacttaagtgcaaaactataatgggtatttggataatcgccaaatctaacagtggcaaaaagttaaattcccc 8478
ttttatattagtccttgaggagctacggcaatgtttgctagcaccggacaagatcatccaataaaaacttagagattata 8558
cataacagtgtcactgttactagcaaaattttccatcctattgcatggttagtacatttttttttgtgagaaatatatgg 8638
tcaatgccatttgcaatatacctccaggctctagtcaatataagactacatacatatgcgatctacattttgtactaggt 8718
ctctaccaaaagaaggggtttgtacccttctgttctcttaaattaatgagacgatttgtaggatctttctttttcaaggg 8798
attgtgggagttctgactctcatggctaacgaggcgctctgaatactagtagtttatttccatccatctatttatttatt 8878
ttaataaattttattgcttttgaatttatatttgtaggtacatcaaggacgagctgaaaactattcaggcattcttaaga 8958
gctgctgaagtaacaaagaagaaagatgacttgctaaaggtatgggcagagcaagtacgagatctgtcatataacattga 9038
agattgcctagacgaattcaaggttcatgttgagagccaaagcttggcaaagcaactaatgaagcttggtgaacgccatc 9118
gaattgctgtacagattcgcaacttaaaatcaagaattgaagaagtgagcaacaggaatacacgctacagcttaatcaag 9198
cccatttcctctataaccacagaggatgagagggattcctacctagaagatgctcgcaatcaatcaggtagcaacactga 9278
cgagtcagaacttgtgggctttgccaagactaaagatgagttgcttaaactgatagatgtcaatactaatgacggtccag 9358
ctaaagtgatatgtgtggttggtatgggtggattaggcaagactacccttgcaaggaaggcatatgaaaacaaggaacac 9438
atgaagaacttctcgtgttgtgcttggatcactgtgtctcagtcatttgacaggaaagaaattctgaaacaaatgatcag 9518
gcaacttctgggtgctgattcattagacaaactcttgaaagaatttagtgagaagttgctcgtgcaagtccagcatctcg 9598
ctgatcacttggttgaagggctaaaggagaaaaggtactttgttgtccttgatgacctatggaccatagatgcatggaat 9678
tggattcatgatattgcttttccgaagattaacaacagaggtagtcgcataataataaaaacgcgagatgctggcttagc 9758
tggaaggtgtacctctgaatcacttatttaccaccttgaaccgttacatatagatgatgctatacacttgctactagcaa 9838
agacaaacataagacttgaagacatggaaaatgatgaggacttgggcagcatagttacaaaattggtaaaaaggtgtggt 9918
tatttaccgctggctatactcacaataggaggcattcttgctactaagaagataatggagtggggaaaattttacagaga 9998
acttccttcagagcttgagagcaatccaagcctagaagccatgaggaggatggtgaccctaagctacaatcacttaccat 10078
ctcatcttaaaccatgctttctttacctaagtattttccctgaagattttgaaattcaaagagggcgcctggtagataga 10158
tggatagcagagggttttgtcagagccacagatggggtgaacattgaggatgttggaaatagtcactttaatgagcttat 10238
caacagaagtctgattcagccctcaaaagttagtacagatggagttgttaagagatgtcgaatccatgatatcatgcgtg 10318
atatcatagtttcaatttctagagaggaaaattttgtgctgttgactagggagaagatcactgttgtagcggaggagagc 10398
atccgccatctagcatttcatgggagcaaatgctcaaagatatgcttggagtggaaccatctgcgctcagtaactttgtt 10478
tggcgacagacctgtggggcgaacacctgcactttgttcaccacaatttaggatgctgagagtgttggatctggaagatg 10558
caaaattcaaattcacacaaaatgatatcaggaatatagggttgttgcgccacatgaaatatttgaattttgcaagagcc 10638
tcaactatttatacacttccaaggtccataggaaaattgcagtgcttgcaaattttgaacatgagggaggcaaatatctc 10718
agcactaacaactgaggtgactaaactccagaatctccgtagcctccgatgcagcaggaggtctggttctggttacttta 10798
gcataatagataatcccaaggaatgcttgatgatcaccatgtgcttaccgatggttttcttaacttcaataaatttcagt 10878
gaccgtgtgaagttaattcctgagatatgcatgtcatgttctacccgttggtctgatacaaagggtgtgagggtgccaag 10958
aggaattgacaacctaaaagagttacagattctagaagtcgtggacatcaacagaactagtaggaaggcgattgaagagc 11038
tgggggagctaattcagttaagaaaattaagcgtgacaacaaaaggcgccacaaataagaagtatcagatattttgtgca 11118
gcgattgagaagctctcttctctgcaatctctccgtgtggatgctgagggattctcagatactggaacacttgagtggct 11198
caattcgattgcatgtcctcctccattcttgaagagactcaagttgaatggatctcttgcagatacaccaaactggtttg 11278
ggaaccttaagcagctggtgaagatgtgcttatccagatgtgggctaaaagatggtaaaactatggagatacttggggca 11358
ctgcccaaccttatggttcttcgtctttatcgcaacgcatatgctgacgagaaaatgacattcagaaggggaacattccc 11438
aaatctcaggtgtcttgatatttacttgctgaagcaacttagagagataagatttgaggagggcacctcgccaacgatgg 11518
aaagtatagaaatttatggttgcaggttggaatcagggattattggtatcaagcaccttccaagacttaagattatttcg 11598
cttgaatatgatggtaaagtcgcgaagcttgatgtgctgcaagaggaagtgaatacacaccccaatcatactgaattgca 11678
aatggcagaggatcgaagtcatcatgacctaggaggtatataaggatctaatatagtatgtgagcaatgaaaatcgcata 11758
ttgagcttgccattgacagatcatggtcctgaccaggccttgcatctgatggcgatgatgcccatgacaatccagcgttg 11838
cgctatcaaacatcatgttgacaacaaagagttcagtcttgcacttgagaacctacccagttattatgtaacgacacgtt 11918
tatatgcattgagatgaaaaatagacttaaataaccatataatgcaagacccaaagagaagagaaatacaatatagactt 11998
attttccagccatttgaacacagcccacaatgagggatgaaaaatatacttattctgtggagaagtacgatgggctatcg 12078
tgcctttgggtcggcccgagtcgtattagccgtgcctgggccgtgtgtgcagcatgttggtcgggccgtgccggcccgac 12158
taaccttggcccaggtacgattgggcttgggccgtgccatgccgggcagcccatttggctaactatactacactatatgg 12238
tactccctccgtttcatattataaatcatttgtcttttttcatagtcaaacttatttaaatttgatcaagtttataacaa 12318
aatatagtaatattttcaacacaaaacaaatattgtatcaaaatatattcaatgttaaatctaatgaaactaatttggtg 12398
tcgtgaatgttgctaattttttctataaatttggttaaacataaataattttaactataaaaaagtcaaatgacttgtaa 12478
tataaaatggaaggagtagtagctactcgtctgttaaaaaaaaagtttcctcctaacataagagcaaattggctcgacac 12558
aattcacctgctatttatctgtctttcgccttgtaaggtagtggtgcgggactgcaggtgcagcatgcgtacacaattga 12638
tctgtttttacttcttgcaatgagataagatggagaacgttgtgtcagtcactacatcttaaactgcctgatgctcatcc 12718
aaaatgagaaagatgttccaaattacacattgtgcattccgatatgaatgatttctcactctatatatactctagatgtt 12798
cagatttttactaagaattgagttggttagttgaatggagatatttttctagttcggtgctttagatatgttttgctttt 12878
gcctaatgactattttttttgttttattaatctatgctatactacttacggttatagatgaatatagttcttgtttatta 12958
tagctcttctgaaaagtgcagtgtacaactagttgtgttattgttaactgataaaacatagaaatgttgatttaatgagc 13038
tacattgatctcttcttcgttgatatgagaagcttggtttgagttgtttttatattctaataaatatttgttactggaat 13118
cgctccattttcgtatttgaaaatatttgattatgttttttatgtggggtttctgattccaattaaaaaaatgaaaataa 13198
aaatggtatgatggtttccgttcgttttcgagccgtttcatccctaattgcacatgcatgcgcggaacaatggatctcac 13278
taatcaagtcgcacgcagttctttatatcttgttgattatggcttgtgaagcatagattcaccgaattaattgggatgct 13358
aaattagtcacatgcatacccaagcttgcatatgacgttatgtagatagagatggccaatataatgcgctggaaagtcca 13438
aagtgaggatgcaaaacatcttatagtgggtagtggagccatgcaaggacctggtctaaagcgcacctaaaccgtaatgt 13518
ggactttaaagttagggggaatatgattctcttcatgtgcacctaaaccgtaatatgcagtgaaacgaacgctatgatat 13598
gatgataagcttaattcctctctctgctcagactgttcagtgcaaaagctaccaacgagcttgtctccttgtgcggtcgt 13678
gagcttgcttgtgctaagcttgaagggagagtcgaacgaatccatggcggagacggtgctgagcatggcgaggtcgctgg 13758
tgggcagtgccatcagcaaggccgcctctgccgctgccaatgagacgagcctcctgctcggcgtcgagaaggacatctgg 13838
tacgtactgcactgctctcgtttatcctagcaagttcttaggctcttaatctcgaaattgaggaacaccatgaaacacta 13918
aaagagagctcgaagactaggaaagaaaactagaagactaagctttgaaagtcttctaaatccaagcatctcgacattga 13998
tcatccttgtgcaacatcatcccttcctattgcttcaccagaatcggtgtcccttgtggagatctctgtcgtagcgtcaa 14078
ggggagaatccgagaagcagaactagtccgcgctgccttcgctacgccatctccgccatagaggatctcatccacgaaac 14158
atccaccatccaaacgggaaactgttttaaacactcgggtggatgttcacccgtttcttgcatatcatctaaatggttat 14238
gaaaaattttcaaaaaaaaaacatgataggttaatatataatatatcatctcacaaatatgcaagttcaaattcaacttt 14318
tataagttgtaagtataacaggacgttcatctcacaaatatgcaagtttaaatttaacttttacaagttgtaagtgtaac 14398
agtacgtccatcggatagattaatatccatctccccatccaaacccgttgttgcaccatctgtcgaatccggctgtggac 14478
gctcggaggcaagagctagctcacccgtcccacacacacacccaacgacgtcacaagcgcctccgaacaacgccaactga 14558
taacttggcagctcctacgtgccgacgtcgcggtacttgccggcgctcctagcgcacgcaccgtcgaaccacaccgtcac 14638
cgaccaactacccaccgccgccgacttctgcctcatctgccatcgtggccctagcccaagttatcatcgtggcaattgcc 14718
gaggctcctaagtgtgccacggccgaggcaaagttctaactgaatcagagtatcagacagccaccaccgacgctacttct 14798
gcttcatctgccatcgccgtactagttcaagttgtcgctgtggcaatcatgggccctcctagcgtgccacccaaccggac 14878
agccacgacatcccccatcactgttgttattgccgcgccctgacccctatcgtcgtcgctcttagcgcgtcgacgagccg 14958
accagccactgtcgtgcagatgaaaaaaaaaacacatattggcctgagagatctgcttagttccagtgcaggtccaacat 15038
gctgtgagatgcgggcgtgctagtcagtttgatcttgcaactgacaagatatataaacagcagataaaacagccgatcga 15118
ctaacaagccgatggagtaattccagccgatagccgatattagccgatgccgattctagccgatgtcgatagggttttga 15198
actatcggctatatgtctaatgtaggcaatgatataaagacaattggctgatgataataaaatataaaaatataatccaa 15278
tagaaaccaatcggctaataataaatattgatccgatggttaaagcatacatcggctaaaagtccgatgtcataaaatcc 15358
aatcgatttagataaacagtgaaaccttttttgcaatcggctaaatccaacttgtatgtaatcttcgtaagccgatgaac 15438
gtccagataacttatcggctagcacctcgataaaacactagcatgaacctatcggcttaacaagatttatattatcaaca 15518
acaatctagtaggtcggacctaaccgatgcaacacggattagatatgataatctaatactcgatgagccaatagatctgt 15598
ctaatgtgatggatataacaaatctatttataacagcattgcgattgtagagatatatcggctaagacagaatatcagac 15678
ctaactaaaccgatgcgtctctaaacacaatgcaattaattagagatataattgagatatcagctaggcaaatatatcaa 15758
ccaaactggagcgatccaagagatcggagcaatgcagccttgaacaacaccaatgtagccgatggattcaccagggccga 15838
cggaacgtaggacttaccccttcgctgaagatcgggctgaaccaatgcagccccgcgtcaggtgccaaattccgccggtt 15918
gataagtaaaacctcagaaaagaggatgacgatgcgccgagagtagtattgatcgagagataaattgcaatgaccctgga 15998
tgtacatatttgtacccatgggtagatattagttcttgtaggacaagaaagaaactttcctaaagataaaatgaaaacat 16078
aaagtctttattggatactaaatacactttcctaaagataaaaggaaactaaaccctgcctaattaatagataaactgcc 16158
atgtcgtatcctccttgaactcggactcttttagataagctacctttaactaatctttacccgaatccatcaagaataca 16238
aatgttggcattgatagtttccatcggtcaattctaggactttgaagccgatactgactctaagccgatgattactttgt 16318
gcttaccaaattttgttgttaacatgtcgcaaccaccatcaccggccagccaccctgatcattgttgttgactcagcatt 16398
cgccaggctgagcagtccacatacatgccgccatctccatggcactgtcgttgccgcccctttctcctagagccgccgca 16478
gcgctcttcgacacacctactgcatcgtcgagcagtcgtgctaccacctcctccatcgaccatagccgcctcttctgctg 16558
caccggatccacccacaccaaccaccagatacagtcaagccctcattcccggatcccatatccatccatgccactactgt 16638
gctgcccagtccaaggaatggagcgaaggaggaagccccgccgctgccctcccggcggccacatgcactccagtgccttg 16718
ctccgacggcagcgaggttggaaaatgggtggcagcggctagggtttatctggggagaaggaaaaggagagggagggggg 16798
ggggtccacttccagcttaattagcctagatcttattgacaaatcagttgctgggtgcacagacatgttattctttttgc 16878
atgaccaatcttgaacacttaggtatgttagttgagtggacactggtctatctgaaacatctcttcacatggaggctgcg 16958
aatgagttttctttttgagagaccaaagtttcgttgtatgttaagtgataaagccttggtaagaaatgctaccacaaacg 17038
aactaataactccaaacgtaaagtggaggaacccgtatgggtgactcgagtggcgacaaactctagcacctccacctcct 17118
tggatgggctgcggcggtgctttcagcatcccagtctccttggaggcatcatctagaattaaggtcttgttattgcttag 17198
catgccttagggcacgtccagtgtttagttcgactaaaacttccatgaaagccaaacaaaagttctgtttgaccaccaca 17278
gtgtaaaaatcgattgtgggacccatgcaaaaaaatcacaatctcagctgcctatgctctcctcctggacctgatagccg 17358
tgcacaacaaatatttttttaaactggatgtgttcggcttctctttaaagatcgttttttcctctgacacttaccaaccg 17438
gctttcacagtgtggtcagttctttttttttttacgcaaagtttgattttagtcagacacgggaggatctgttaagcagg 17518
cttagaaatttcggacccctccaatacaatattattttagccaaaattttctaattttttaatttttcatgaattttggt 17598
aatatttgttctaatttaactaaattttgttcaaaatttcggtctatcagtgacctccgatcaaatcagttaaaccgaga 17678
aaataaaccatgctcttaagagagtttggtatggttcaatatcaaaacttatagtcttgcaattttttctaccctttatc 17758
tttttccctgactatttagtatggatcgtttaaaaaaaagaaagcccattggtgaccaagggcttgtttgattcaagacc 17838
atccctagccttaccaaccttttggcaatggcaaaaattggttgttgccaaaaatattggcacaaattggctaagcctat 17918
gattggtttctaccaaagttgaattttggcattcaatcaagccaaataatttggcaataacattttcttatctatggata 17998
taacatatggcaaatattttggcattaccattttctttttgccaaacatgttattccttttgaatgaccaatcttgacac 18078
cttatgtatgttagtagtggaatcgacactattctatctaaaacatctctttacatagaggccgctaataatttttcttt 18158
gagataaccaaattttccttacaagttaagcaacaaagaccattggtaagatatgctacgacaaatgaactaataactcc 18238
aaacgtaaagcggaggatcccgcatttcccacgtgggtgactcgagcggtgacaaaccctagtacctccacccccttggg 18318
tgggttgtggtggcactttcggcaccgtattttccttggacggatcatttagaaagtcctattattgcctagtatgcctt 18398
gacagtttaggcaacactcttggatggtggtgtcctttgccctggtgatctagtagcccatggatgtttagttatttgga 18478
catggtgttggatggtgcgctcgtgggcctgttgtaggtctggtgccaaccagtcatgcttagaaatagccggataggtg 18558
cacagtgctagttctttacttggtggtttgtgcagcgctatcgacatgtggtggtgtgctttttctttgtccggataata 18638
atctcatagggctatactcttgttattttgctgctatattattatgataacttggtatggttcgttttttctttttttgg 18718
aaaaacacctagttgatcaagggcttgtttggttcaagtgcattcctaatcttaccttttcttttttttttttgcaatgg 18798
caagaattgttcattgcaaaaaaaaaaaagagataaaaattggctaggcttacgttttggttcttaccaaagttgtactt 18878
tgagaccaaatatatggcaaaattttggcataacctttttttttttgcttggttgagcttggtacaaaccaaacagtcac 18958
aaaatagactgtcatgaatcacgcctactaaattcctttgaaccgaactagaatatatttgctcttaaaagatttcttga 19038
tttcaattggtaccatttactagtagaaacttaaatttaaattttaaaaacaaaatcataatattgttgttatggaaatt 19118
ttagtcattttagtacttttgtaatatatgagttgggttatacttgagatatcctaaattgctttaagatgaacaattgc 19198
taggtatatcaaagatgagctaaaaacaatgcaggcattccttagagctgctgaagttatgaaaaagaaagatgaactat 19278
taaaggtttgggcagagcaaatacgtgacctgtcgtatgacattgaagattccctgatgaatttaaagtccatattgaaa 19358
gccaaaccctatttcgtcagttggtgaaacttagagagcgccaccggatcgctatccgtatccacaacctcaaatcaaga 19438
gttgaagaagtgagtagcaggaacacacgctacaatttagtcgagcctatttcctccggcacagaggatgacatggattc 19518
ctatgcagaagacattcgcaatcaatcagctcgaaatgtggatgaagctgagcttgttgggttttctgactccaagaaaa 19598
ggctgcttgaaatgatcgataccaatgctaatgatggtccggccaaggtaatctgtgttgttgggatgggtggtttaggc 19678
aagacagctctttcgaggaagatctttgaaagcgaagaagacattaggaagaacttcccttgcaatgcttggattacagt 19758
gtcacaatcatttcacaggattgagctacttaaagatatgatacgccaacttcttggccccagttctctggatcaactct 19838
tgcaagaattgcaagggaaggtggtggtgcaagtacatcatctttctgagtacctgatagaagagctcaaggagaagagg 19918
tactttgttgttctagatgatctatggattttacatgattggaattggataaatgaaattgcatttcctaagaacaataa 19998
gaagggcagtcgaatagtaataaccactcggaatgttgatcttgcggagaagtgtgccacagcctcactggtgtaccacc 20078
ttgatttcttgcagatgaacgatgccataacattgctactgagaaaaacaaataaaaatcatgaagacatggaatcaaat 20158
aaaaatatgcaaaagatggttgaacgaattgtaaataaatgtggtcgtctaccattagcaatacttacaataggagctgt 20238
gcttgcaactaaacaggtgtcagaatgggagaaattctatgaacaccttccttcagaactagaaataaacccaagcctgg 20318
aagctttgaggagaatggtgaccctaggttacaaccacctaccatcccatctgaaaccatgctttttgtatctaagtatc 20398
tttcctgaggattttgaaatcaaaaggaatcgtctagtaggtagatggatagcagaagggtttgttagaccaaaggttgg 20478
gatgacgactaaggatgtcggagaaagttactttaatgagctaatcaaccgaagtatgattcaacgatcaagagtgggca 20558
tagcaggaaaaattaagacttgtcgaattcatgatatcatccgtgatatcacagtttcaatctcgagacaggaaaatttt 20638
gtattgttaccaatgggagatggctctgatttagttcaggaaaacactcgccacatagcattccatgggagtatgtcctg 20718
caaaacaggattggattggagcattattcgatcattagctatttttggtgacagacccaagagtctagcacatgcagttt 20798
gtccagatcaattgaggatgttacgggtcttggatcttgaagatgtgacattcttaatcactcaaaaagatttcgaccat 20878
attgcattgttgtgccacttgaaatacttgagtattggatattcgtcatccatatattcacttcccagatccattggtaa 20958
actacagggcctacaaactttgaacatgccgagcacatacattgcagcactaccaagtgagatcagtaaactccaatgtc 21038
tgcatactcttcgttgtataggacagtttcattatgacaactttagtctaaaccacccaatgaagtgcataactaacaca 21118
atatgcctgcctaaagtattcacacctttagttagtcgcgatgatcgtgcaaaacaaattgctgaattgcacatggccac 21198
caaaagttgctggtctgaatcattcggtgtgaaggtacccaaaggaataggtaagttgcgagacttacaggttctagagt 21278
atgtagatatcaggcggaccagtagtagagcaatcaaagagctggggcagttaagcaagctgaggaaattaggtgtgaca 21358
acaaacgggtcgacaaaggaaaaatgtaagatactttatgcagccattgagaagctctcttccctccaatctctccatgt 21438
ggatgctgtgttattctcaggtattattggaacacttgagtgcctagattctatttcatctcctcctcccctactaagga 21518
cactcaggttgaatggaagtcttgaagagatgcctaactggattgagcagctcactcacctgaagaagttcgacttacgg 21598
aggagtaaactaaaggaaggtaaaaccatgctgatacttggggcattgcccaacctcatggtcctttatctttatcggaa 21978
tgcttaccttggggagaagctagtattcaaaacgggagcattcccaaatcttagaacactttgtatttacgaattggatc 21758
agctaagagagatcagatttgaggacggcagctcacccctgttggaaaagatagaaataggcaagtgcaggttggaatct 21838
gggattattggtatcattcaccttccaaagctcaaggagattccaattacatacggaagtaaagtggctgggcttggtca 21918
gctggagggagaagtgaacacacacccaaatcgccccgtgctgctaatgtacagtgaccgaaggtatcacgacctggggg 21998
ctgaagccgaaggatcttctatagaagtgcaaacagcagatcctgttcctgatgccgaaggatcagtcactgtagcagtg 22078
gaagcaacggatccccttcccgagcaggagggagagagctcgcagtcgcaggtgatcacgttgacgacgaatgataggtc 22158
agtcactccctacatggcagcttaattaacttgtttctaattctcttcttgttcagtattagccatcaggtgagggcgat 22238
gatttcaactcacttttcatctctctcgttttcttaacctgacagcgaagagataggcacagctcaagctggctgacgat 22318
ctcctcccccatcagcgtcgtcatcagcgaacagatagggcagggcttccctgcttctgcgtgcacctcactgctctgac 22398
tcggagggacatgatgatcaatgaggcttccagttcccaaatgtgcgcctaacacactcagtcattcccatcccaggtat 22478
gaattgatcgatctgatttattccttgccgtgaggtgcaaatgtttgatgcttactttgatcagggtattctgtttcggg 22558
ctgtgatatgcacaattactctcaattcgtgttttgatgctgagtttttatttctcttcttacccgtgcactcttcattt 22638
ccatttcattcataacagaaaacaatttgattgcattgtggaagggaatataagatcagaaatcagatggttagttgtgg 22718
ttttcttatttcgtttgctatgagcaccaatcgttttgctagaatgtcttttttttgaacgactcgcacgagacggtgcg 22798
atgttctattgatagagcaggaaaagaattacaagactacaaccctggagggtcataaccaggaaaaagaaaaaatatac 22878
caccacccacacaccggcagcgccaacacacacgacaggagaaggttagcaccggaccggccgccgctagaccaacaaag 22958
gagcacagacgagattgcacgaatccacggctgacgcaccgatgctccaccaccactcaaactctgccgacatgtggggc 23038
ccctgcaccgacgccccccgccggccagcctttgcgcgccgaagaccgcgccacacccaccggtagctccaccacgcacc 23118
gaggtcacctcttccaccgccggccgcgcctctcgcgccaagccggcctcctccaccggacgcgcctctcgcgccaatcc 23198
ggcctccctccatcggctgcgcctctcgcgccaagccggcctccatctctgccgccggcgcctctcgcgccgagccggcc 23278
tccgctgccatcggctgcgcactctgcgacaagccgttctctgacccctcctccaaacgatgctgcgccggtccgatgta 23358
gccgtctgccacgccctcggcaagccgtccgaggccgccatgcctcctcccaccaccgtcacggtcaccatcacctcacc 23438
gtcgccgccgtcacctcacaaccgactccttccccgcctccactgccgatgtcgccgccgcctcctcagtcacggccgtc 23518
gcaagccggccgtcgtcgccgctgccactgtcaccaaccaccgacgccagcagccgccatcgcaaaaccgccgccacctc 23598
caacgcagccgccccgatgtcgtcgccatgcgggggggggggggggggcaaataggccttcagcacgctagatccgcccc 23678
acggggggccaaaatggcctgcagcgcctgatccggccgccgtcgccagcctcgccgccagagccacccgccgtcgtcag 23758
cctcgccgccacctccatccccgccgccatctccatccccgccgccggcgccccctcaccatccccgacgccgtcgtcgc 23838
cttccccgccgccagccgccaccaactaccgccgccacctcgccatcttcaatccccgccgccaccaccaccaactgccg 23918
ccaccaccttaggccgaccctccgccggcggcctccccaccagatccggccgcggcggcccggatctgggtggccttcgc 23998
tgtctcgcgcagccccccaccgccgacgggagaggcagacgaaggcgacgaagccccgccgccgccgtccttgcggccgc 24078
tcgggcggcggcgaggcggcagagggaggaaggggggagcggcaacgccggctgttttcgcctcccgtgtcgcccgtgag 24158
ggagggcgacgcgggggcctcgatgagtttttttgctagaatgtctgaaagatcttgtgtacatatggtggactgaacaa 24238
ttgaacattactagttatcatattttatattgttgctaaccgatcacatagtccagtactccagtagtaagatttttgtt 24318
aagtttattgttactgaatatattgtttggcctgcagttgttatttctctcaaaaaaaaaataatttgttagtctcaaat 24398
acaaaaagaatgacagatcaaacaagttgtttcactctactagtttcaaattgatcatctctgtttgttcttcattcatt 24478
ttctctctgtaagagagtttgatagggtggagatgcggtcatgtggatgatcaaatggactacatcagacgcatcacact 24558
gctgcccaacctttacgcactgttgacaaatggagtgcaggtcctaaaccaggccagaagtttgttcagtgttcttgttc 24638
caaaataaacattctggatggcaggttaatttattataacattcactcttatagctttcttagtcaaaactacaaataag 24718
gtctcctaaaaaatgcatcgacgttgatatctgtgttttctgccatgcagaatgacttgctctcaatggttgaagctgca 24798
accttccaggctcatttctggctgaatccaaattggtcgcttggaggctggagctgcatgacatcagagatcacaatgcc 24878
actttttgttgccatgggtgagcgtacatggatcatgccgctaagcctttactccgagctagcagaaaactggctggctg 24958
tagatcgagttcgccatcgccctcaactttgtcgatgtgatggtgatcatggatagatgtatgtcacagcgcaaagcgga 25038
gactccaatgttagcaaccgtactgtaaatgtggaggggaaaatttctcaaggggacaaccatggaacagaggagatgga 25118
gcaaagtagttgctcttgctatcatttcagatctcaggctgatctctagctctgataggagtactaattagtatgtgtgt 25998
gtatttaatcattgatggtacgttttaagagttgaaccagatattgcttgatcattacatgacgatatgtaaaaaatggt 26078
attgacaaggagacgcaggaacagggtggttcattgctctgtttaatcttaatcttggagagctaggatggaaaactgag 26158
ttggtggttttatctctactacttttgatgttgtccaaaacaaatttgtttctactcatgtacgtatgtaaaaacacaaa 26238
ttttagtgattacagaacttatttttctctttaatcagattatttatcagtgggatttttctttctctagtagtactgta 26318
taaaaatacttttttatcgtcaatcctcctaaaattcctatttataaactcatttttcttcagttttttttctgtatttt 26398
gattctctttcctctacgtgactacacattttgaattgaacatgctctgtatctgctcggaatacttattattcagccaa 26478
cttaaatgcatgagatttgctccggtccaccaaaaattacctcgaggtactagtacctcatggtaccaaatcgtttccga 26558
tcgtgaaacgatttggtaccgtcagttaccgtacctagagatctaaatgcataggggcatttggaatgtgcacttaacag 26638
ttgtttgaacacttccaaggaatttctcctcagttgtcggacgactcattcattttcatcgcttccaacaattatcaact 26718
gtgtctcttcctctccccctagatccggccctaccaccgccaaagctgaccggcgtctgcgcggcggctaggacatcggc 26798
gacggggaggtgggaggcggaggcacggcgatggtggccagaggcagccgggctcgtgcgctgaccgtctgggttggtga 26878
cggctagaggtggtcgggaggacggcgggtagcggctatgcgtcggcgacggaggaccttcgaacggcggtgggggcagt 26958
agcggaggacggcgtcggcccaggacgacaacggctcccatcggtgccggtggccctaggggctgcggcggaggacgacg 27038
gttgttcacgtcggcggccgtggccctaagggcattggcggcttccgtcggccggcggcccaggatggcagcggttctca 27118
ttggtgccggtggccctaggggctgcggcggaggacaacggcagctcccgtcggcaccggtggccctaggggtagcagcg 27198
gaggacggtggcggttcccgtcggtgttggcggcctgggatggcggcagtctgagtactatggataatggaggcctagaa 27278
atctggcaaagtggaagctaaccccatcggttcgtatctggttggctagttctaactaaacgatgaacaacgacggttga 27358
aagtgtgctaaatggcagcacgtagagtcaactcacctgaaacggataaagatggcaccaacggaggtttgcatcgacgg 27438
cgcactaggggcaatgacgaatagacaacttccactcgtcaaacctggttgtttcataagaatatggtgggaagcagaga 27518
agctgggatgtgtcgaggtcgtttggttttcttttcttttttgattgtgtgttctcctcgttgttgaggtgtgagtataa 27598
gtgctcttgtatccttttggctgtgtatatccttcataatatagaggccagattaatgaaaatccattatctaaaaaaaa 27678
aattgtcagacggctaattaaacctaagaattccatgtgcaaggacttgtactagtgttcccaaatcttagaacactttg 27758
gctttacaaattggatcaactaagagaaatcagatttgaggacggcagctcaccccagttggaaaagatagaaatctctt 27838
ggtgcaggttagaattagggattattggtatcattcaccttccaaggctcaaggaaatttcacttgaatacagaagtaaa 27918
gtggctaggtttgctcagcttgagggagaagtgtgcgcacatccaaagcatccagtgctgcaaatgatggaggatcgaag 27998
ctatcgcgacctaggaggtgatgccgaagtatctgctgtacaagtgcaagcaggatcccctccctgagcaagagggagag 28078
agcacgcaggtgatcacgttgacgacaaactacaggtcagtcactccctacatggcagcttaattagctagttttctctt 28158
cttattcagtattagccgtcaggtgatatcgatgatttcaactcaccctttcacctctcttgttttcttaacctaacagt 28238
gaacagataggcacagctcaagctggtttgatcaagtcatcatctcctcctccattggcatctcgggtcgtccctggctc 28318
cctgcttctgtggctccgagcaggggtgctgatctaaggaggcatccacttttttcaattgcgtctcaggtatgaattgt 28398
tcgatctgatcttttctcgtgatatgttactgttccagcatgagtatttcaaccagcggcttagaatttttcgtttgatc 28478
aggttttttccctttcttacctgggcactatacttttggttttcttatttcatttttgctgtgcacaccagttgtttgct 28558
agaatgtctgaaagagcttgtgttcatggttagctcaacattatgtgttagtcatattttatatcgttgctgacctatca 28638
catagcgcaggagtttttttgctatgtttattgttgcttgctgttgattttcctttttgtaaagaatattagctttttag 28718
ataacgaaaagaatactacgtatcctgcaaaaaggattgatttggtcgtcactggctagtaggggtgaaaacggtacgga 28798
aactttccggattccggacctattttggaaacggaatctgtcggtcagaatttttttggaatttttcggaaacggaaacg 28878
aattcggaaatattttctcggaaacggaatcggaaatgataagggcagtttccatcggaactcggaatcggtcggaaact 28958
ttctggaaattttctcggaatttccggaaattttgtgactgaaatagtgaataccatggtatttggctgttatttttttt 29038
aaagtatttgttatgcaaatctgaagttacataagaatatttttttcctgcattaggatttatcaacatcagtaatctct 29118
taaacatagataatttatttcatagattgtgttctgtgattgagacttaaaaaatagacttatatgattgtgttttatga 29198
tgaattgttgaccgttgagacttgagaattggatttatcagtttgagaggttttttattccgataaattttgttaccata 29278
ttcgtgccgattcgttttcgctccgttttcggtttcgataatatttgattccgttttcgtatccggagtttccgattccg 29358
attgcgaaaaaaatatgaaaacgaaaacgataacggtggtttttgtccgtttccataccgccgttttcacccttactggc 29438
tagtacaaaacaatttggattgtcggagttgctggtcttgggaaactactcttcaaaggtggtttacaaaactctgatgt 29518
tagtaccaagtccttgcagggagagatatagaagagtgggaaattttgtatgctcaacttccatcagaacttggatgcaa 29598
cccaagccttgcagcaatgaagaaggtggtagcccttagttacaattacttgccttctcatgttaagccttgctttctat 29678
acctttgcatctttcctgaggattttgatgtccaaaggaagcgcctagttcatagatggattgcagagggatttgttaga 29758
gctaagggtggagtgagaattgttgatgtgacagagaaatattttaatgagttgattgactgaagtatgattcaagcatc 29838
tagagtgaacatagacaaaggtactattaagagctgccgagtccatgatatcatgcatgatgtaatgatatcaatattgg 29918
aagaaaattttgtatatttgatgagggatgatggaactagtgtagtggaggaaaaatattcgccatgtagcgtaccatga 29998
cagcaagtgttctattataggcatggactggagccatgtacggtcgttaactttgtttggcgatgagagacccaaagagc 30078
tctcacctccattctgttctccccaattgaaaatgctaagggtgctggatctactagatattatatttggactagcaaaa 30158
agatatggataaaatatggttgttgggtcacttgaaatatgccaatattaggtgttccaatgaatgctcaagcatttatg 30238
cacttcctacggcccgtttggttggagggagtttttagtagggattgagagtttagagggatgaggctattaagtgtttt 30318
gtttggttgggagacataggaattttggtgggatggggatggggaattgagaaaggaactccctccttttcaatacctgg 30398
gtagggacaggtaattgggagggaattcctcctctactttatctaagcaaatcacagccatccatttttattaatgacct 30478
aatctccaactaaactccctatcaattccatcagctctaccaaacaagatattgggattaaaaatcaaattcccatcttg 30558
atctcgtcataaattccctcgtgtaaattcccaatccccttccctcgagttaccaaacaagccgtagttcgataagaaaa 30638
ttacaagagttacacattggacatatctgacacttatattacaacgctaccaaatgagattagtaaattgcagtctatgt 30718
gtcctccgtggtagaagacaaggatcctactatgaccttgatacatataatcgtaaggaacgtgtacttattttatcatg 30798
tattcctttgattatggctttaagtgattctgataaccatagaagactaattaccgatctacacacgggttgttcaagtc 30878
attggcatagaattaaagatggtgtaagggtaccaagaggaatcaagaatttgaagagattgaaagtactagagatagtg 30958
gatatcgcggtaactgacagcaaagcaattcaagagttgggggaacttaaccagctaagaaaactaagtgtcatgacaaa 31038
agggtcaaacaagaaaaagtgcaaaatactttgtgcagccatcgaaaagctcacttccttcaaatctctctatgtggatg 31118
gtgatcatggatactcacttgatggaacacttgagtgtcttgattctatttcccatcctccttccctcaagagccttaga 31198
ttgaaggggtgtattaaggagacacccaactggtttagggagctcaaacacttggtgaagatttacttatataaaagtca 31278
cctaaatggagataccatggagatactcggggaactacataatctcatggatcttcactttcgttggtatgcatacgttg 31358
gggagaagctagtgttcattgagggagcattccaaaatctccggaagcttgttgttgaaactgaggataaactaagagag 31438
gtgaggtttgaggagggcacctcaccccagatggaatggatagaaatctgtcattgcgaactgatatcagggattgttgg 31518
tgtcaagcaccttccaaggctcaaggagataggactcaaatctgctaaagtggcaaggcttggtcagctggagggtgaag 31598
tggacacacaccccaatcggcccatattgcgcctgtctgagaagcgaagctatcacgacttgggggaaacccatgtatct 31678
gttgttgaggtggaagtggcggatgagccccttctcaccagcagcctgtggacgttgacgatcgaacaacaaccagtcag 31758
tcctgcattatgacattcatgcagctacttgttttgtttttctcttctgttcagcactagcttagcttatctcattttct 31838
tacttttttcgttcttccttgttgtctctagcaaaacccaatagttagcgaagaatgctcgatggtgccgatctcgcctc 31918
tagatggtgatgatgatcagttctccgttgtgagctctcctggctgcctgtagtgctgatcccggagtcccctgctgcat 31998
tttccacctcccgtgcctgaggtcagttcagtatctgccctgagtcaccatgccggtatttgtttgtttctatatgattt 32078
gattagtaggatgctttttgtttttgaaattttgtatcatgattggttggagcgtgtgattaggtttcttacaggtgcag 32158
cagaggtcgtattttgttttaatgtgcacaccagatgttcgtccaaatgtcttgtcaaaatttttttatcttttgatttg 32238
tcaagtatttatgattcgcaatatgaaacatcgttggtcaggatctgtgctgcacgtatcgatgcaatgtaatgatccaa 32318
gttacggttccgtttgttcgttatcttcttatcaatttaggccctgtttttttttcagcttgggatttttataatctaga 32398
ttattgagtcagattactataaactagactgttataatatgtagtagaataaacggttagttgtttctttcctagattat 32478
tagagcctagaatattaggtttgcaagtctaaagagggagtggggtggcatggtgggtaatttttcacccaataatctag 32558
aaaaagctcacctaaatgagcttatcagattagagtaagctgggctccagattataataagctacttcaataagttgtct 32638
gtttctttcagcttactcccaataaactggattataataatcccaatctgaaagaaacagggccttagtgttccgtacca 32718
gattttcaaaaaggaactaatatttcttaccaaccatttctgatgtaatctctctatccactgtcataccgtgttacctg 32798
tagactgtagtctgtagacataaaaaaagtacctttttggtctctgaaacatttacctgtatcgactggtctccaatata 32878
tccttttgttttcattcaatttgaaacggccttctttttgccaatccattaaagaactggtttgcgaatgtttagtttga 32988
gagtagcttgaccaaatgggccttaagcctgattgcagtggattttacttgctcagggtaaaaccaaattaaaggatcat 33038
gttcattctcaggaatctctagcatggaatgagaacaaaaatcataacaatggtcaaggtaacaagactgaaccaaccac 33118
catggcgctaatcatcgatctgaaataacatcggaacttgaaagcttcagaacgtccatcgtctattcgtcagtgcgtgc 33198
aaactctgaagtctgaaccgcgaagtagtcatcaaggtcgtagtctcgtagatatgatgttcgttaccaatacctgtttc 33278
taatcgattcctttatgtttttccgttcattttctctcagttttatggaacactattcagctggggacgagatgtggaat 33358
actcaacggagtacattagacgcatgaatgcatgcatttgatgcatagaatacgggtcttggagggatacgcgtaggcta 33438
cgatagcataaattaaaattttctaccgcgtaaaccaagaaccatacaaatattggatcatagatcgttaccacttgacg 33518
cgctgcgcagcggaagaaaatgctaaaaaggcgaaggaaccctcgcgatgtagcgcgcgtcgatgttgaagaagtagtcg 33598
atcgcaccggctcgaccttctcctcctcatgcgttctcctcgccgtactcccacgctgatcagtatcgcaaagcagtggc 33678
gcctctaccggtatccacacgtatagggacggaacgccatatgcagatgtgctagcacccgcgcgcggctagggttttgc 33758
tcggggaaaggagtggcggctagggtttgtcacgtgatgcaatgcctccgccggtcacacccctcacgaatatataggat 33838
ctatgactcggttctccaaggcccgtgggactcctattcggatccctatccgaattaagctcatattggatctccatcca 33918
atccccttattccggctcattaagtgtgcggccctgtaggttcatgcacactcgactgtaacccgaaaactctttttcgg 33998
tccacgtgtcaacagcggcccctaacagaacgtattgactgaccgggcatacacaaagatcatatcggttgaacctctag 34078
tgtatacttgtatgaacctctttgcctcacgatatcgattaagctcaaggctagatatgtgccatcctctaatagctcaa 34158
tcattcactcgaacctgtgatagattacataactcgtgactgtcctcaaccacctttggcatggccatgcatttccataa 34238
tctataacatcgagggacccagagatatctctctataggaggggcaaatcccatcttgattattcatatctcactacacg 34318
tttcatagcatacccgaaaattacttttataactacccaattacggagtagcgtttagcagtccctaagtaagctactac 34398
acatgttggaaaccatgataatatcaggtctatggattcaacaccaacactaaatgagatcactgatgacacaacacata 34478
tggctcttgcagtgtctcatattgggtctatccaacaatatgtttcccaacatgtgtccacattattaatttggtatctc 34558
tataccatgatccataagacatgatcatcaattaatacatgtgctgatcattaaacatatttgttccacatatgatattt 34638
gatcagggatcttttagaaatagcaacatacaacataaagagtctcataaaagaatcacatattcagtaaccaataatga 34718
gttatctattttaaggaacaatgtcggataaatatgtaaacataatatatgatacaatcatctctattattgcctctagg 34798
acatatcaccaacaggtctgcaatgcagtgatgatgacacctgtgcaaaatatttcagtcagggaactcaggatcactaa 34878
aagatgtcgatgttgcctctctggaatgtatgacatctcacttctcaggaaggattggagccggtgccgccatcagaaat 34958
cacaacggccagatttctgtgtcattgagatgctatatgtatcggaagacttcagataaaaaaatatcttctcatctcca 35038
ggttactgaacaacggagagtcattctactcctatatgctacttttaactttcactagctagtctgtatgattgatttgt 35118
ttcctttttttttttgcacttacatttgttaatgtatatgtgtgtatatattaggatagagggcccattttggacatgta 35198
tatgtgtactgttgaactaatgttgaccagaatatctgaaattccccaacattatgaattgcgctggatctagatcacca 35278
cgagaagctaagccggttgtgttggcgggattggtcgcttcaaaaactagatgataccccgcgcgttgctgcgggatttt 35358
gtaggtagaaatggagagctacacgtggaggcataagagataaatgtaatagtagccatcggaaatggtaatttttttca 35438
aacaaacaactatttctgtaggatgaaatctataaactggagtagcaacatccgtgaaggttaagatagcatttctcaga 35518
tatagtaatagcaacgcaagagatactaattttgattattggtattgaccttctcttcagttagactgcatttgaggtaa 35598
tccattttttatgtaagaaatgttagaagcatatgagggtcagagatagcttcagtttgttgaggttatagaagaatcca 35678
tttgagcaactgcatccatgttcacttgtgaagaacagacaaatgaaaaatgagcagcatctcgtttgaaagtttgagga 35758
ttcctgaagggaagagcaataaatggcaaagtatagacaatacgacgatgcaaaagaagctagaagcagtttgcatgaca 35838
acaaagaatgcagacggttaacatgacaacagagaatgcaaggtaccaggaaggcaggaatagagtaatagagaaaataa 35918
tgtaataggagtaacatttattaattggtgattaatgataacctgctggttggttctcgttgaatatccatgtgttggaa 35998
taagaataaagctgttgagaataggagaaatgaaagggagttgtcataaggagaaacataatggtgtatgtgatacttat 36078
agaggttgagtagtaatgtggtaccgaatcgtggagaatagtgaaaataatatctgatttattgttgactgcttattgaa 36158
ttagcattgaacagtacaatcagagtagctaattgaatcgtggagggtgtggccctttggctaactttttatgcacgttt 36238
atgcatttttattaatttattggatcaacatagtgcaacccctatctatccctttaccataaagtaaattcaaagaagaa 36318
aacaatgtttatcttctcatgttaagtgttgttagaatgtctgaatcagaacccatcttaattttctctgtttcttagga 36398
tatcaatcatgtcttagagctgaaagtttgtatcaacagaaacacaataacttgtagtattttgggctaaaagatccata 36478
aaactgaagataaatagtcagatggccttgatgaataacccaatgtttgacaaactgttcaattttgctattacaaatag 36558
cattctttatactctagccagaaaaaaaaaatgaaaaatggatataaagtaggatcatcatataccaaagaagaatcagg 36638
atagatatatcaaaaattagggatcgatgtgtatgaaaaatagctagacagtgttgttatggaaggaataacttgtacct 36718
cttgcgaagcaagtgtcgcagtatgggcatataataatatatagaaatcaagttaccatgaatgaatatggggcaattat 36798
gattacccaatgtatatacagattgctccagctgttgctttggatgggaatgtcatggggactaatctcagcggtagccc 36878
atagttgctgatgcaacaatagtaagcaatctgctaatggaaaacaatctgcatccggaattcataaggtatatactgcg 36958
tgatgtaaaattggtatacataccaatgatcctcataccatgaattgtctcctttagtgaagtgtatatactaaatttcg 37038
cataacatattttcgtataaattattagctgagtttcatacaacactaaggctgcattcgggagtgaggtttggaaatcc 37118
tcactcccgggcacgtaaaacggagcagcgattagggcgtgattaattaggtttttgctaattttttttttcaaaaaagg 37198
attaatttgaattttttaagcaactttcgtatataaactggaataccataagattgacaaaattaaaggcggtacactgg 37278
aatacaagctccataactaagagagtgtttagatccaagagtgtaaagttttgacgtgtcacatcggatattatataggg 37358
tgtcgtatggggtgtttggacactaataaaaaactaattacagaatccgtctgtaaaccgcgagaggaatttattaagcc 37438
taattaattcgtcattagcaaatatttactgtggcaccacattgttaaatcatgaagcaattaggcttaaaagattcgtc 37518
tcgtcaattagtcgcaatatgtccaattaattatttttttacatatatttaatactttatataggtgttcaaacgttcga 37598
tgtgatatggtgtaaaattttgaaatgggatctaaacatgccctaaaataattcttgagcacaaaaatcctcaacaattt 37678
aaacctgcaatatttaaggtattacaaacaatactatggtatcatatacagcgaaaggtcatcgtggccagatgggccga 37758
cctggccagcttcaactatcagcaaaggtaaggaaataagagccgtcggaactacagatctgcagcagatgagagagaag 37838
agcagctggctacagatctgtagccagctgcagaacggactccaagacataatgtgtgtatgacagatgggaccatatat 37918
taatagtatagtaaataactattgtataaattggctattagattggctatagattaattggagctagtaatgggctgtac 37998
tattaaacttgctctaatgataatcctctctgcttgaattgttcagtgtaaaagctagctacaaattctgattgcagagc 38078
cagaagagccagtcgcattgtcctatctcctttgcggttgtgagctgcttgctgttctgagcgtgaaccgtgaagggaga 38158
tctagtcgaggagagtcgatggcggagacggtgctgagcatggcgaggtcgctggtggggagcgccatcagcaaggccgc 38238
ctccgccgctgccgacgataccagcctcctgctcggcgtcgagaaagacatctggtacgtaatatgtactgtggctctcg 38318
tttattctgtactagcttactgatcagcatttattgtatcgacatccttcttagcctcgattttgttggccatcaatcct 38398
gatcggaacaaatcactaagtcaagcaaatcaattgatgagtgcacaaactttttttttttgcatgaccaatcttgagtt 38478
cttgaggggcgaaacagacgcacccgaccgcagtcaccctctccctcctcccacagtcccactcctcccctccgccttgc 38558
cgctacccgagcgaccgccggaaagccaagtggctgcaaggacggtggcggtggggcatgctctctctctctcggcgctg 38638
caaaggagggggcaccgtcgcatctaggatggcaaaggcgtctccacgccagtcagatccggtgagtggctggcagaaaa 38718
cgaggcggaatagcggagagccgagccagtgcttggtgtggctagcaacatgggaggctagcgatgcggtagcaagcagt 38798
ggtggtaactacaaatctatagctcccttgccagatctgacgaccccgcaaccggatctggcgatggcccaacggtgtcg 38878
gaggctacgcatccgagaatgttttggtaaggcgacggcacagtcgtgcgacgaccggtaatccatgggggtgaggacgc 38958
aacaaggtgcaaccaaggaagcaccgtgacatgccgaggaggatgcggcggctcgccatgagtggatctggaagcgacag 39038
atgtagctagatttggtgggcggggtcgcagcagcaacatgtgccgccgatgcgatgtggcagtggctgcttgagatgcc 39118
agatggggcccatgtgatggaggccagaggagcagccagtgggccgacggggaggatgcaaggtgtggtgggctgcagcg 39198
acggctcggtgcgagcaagagcgacgtctgtgcatgctgggacatcctagcggggcggcgtgatggtaggaggtcggcag 39278
acaaaatagcgtgccacggaaggtggggccatggctggtcggcggtggggcatcggcgcagcgaacccacaggctagcgg 39358
aggctgttggggtggtggaacgcaggggtggtgatgacagggaagggtgaaaatctagctcggtgtttcgtcgggccggc 39438
aacgatgacaccttcaagcgccgtcttccccctttggggcgttgtcgagctatgaccctctccctcctcacaggactctc 39518
cagatgaaaacctagtccatttagatgggtcatggcggcaaccttggcatcatgatcttcttggaggcattgtccaggag 39598
gtcttgtttcctcaccttgcaatgccccgatcatttttgtctttgggattctttcagtcgttgtcatcgggttaccgtga 39678
gggacaagtggattgtcttgtctctctcgccctctcactcctcaacccttctacgtgataatgattgggagcccgttgat 39758
gaccttctatttggatctagtgctcggtggggcctttgcaacctagtgtaggactagcggtgatcggtcacgcatagagg 39838
cggtcggttcggtgctagtgcttctcttggggtatgtaaggagtcgctagaatgtggtggtgtgctttttatttttcctt 39918
tccctgattataaccttctagggctgtaattttgtttttttttcttgctatattaatatgaaacttcacactgccttgtg 39998
cggctcgtttaaaaaaaaaacttgagttcttgacactataagtatgttagtagtggagtcgacattagtttatctaaaac 40078
atctcttcatatatatgaaggccactaatgatttttctttcctagggaaccaaagttccgcggtatgttaatccacaaat 40158
ccccttgtaagatatcctatggcaaatgaactaaaggcatgtacaatgataataattaataggagaatcttaacatttct 40238
aattagttctaattaggaatattaactgatatggaagagagagagagagaaagaggagagatagaacattgttgttacgg 40318
ttaacaatggctcagcaactacttgcctctttaaaatggaaacttggttgcgagggtgaaaaaaaggaaagaatattagt 40398
aaagaatatattttttgtttagtagttaaaatatttgttgtctcaattgtttaagggcacactctaaataattttgtgtt 40478
atctaagagtccataccatgcagaggacccgcgttttccacgtggacaaccagagcagtaacaaaccctagcgcctccac 40558
ccccttagatgggctgtggcggcgctttcggcattgcgttttctttggaagtatcatttagaaaatcctattattgccta 40638
gcctgccttaaggaagttcaggcgacacccttggatggcgatgtccgagagcccgtggatgtttagttgtttagacatgg 40718
tgttggacggtcgaatggtgggcctgttgtaggtatggtggcatctggcaaccagtcatgcttagcaatagattcctatg 40798
cagaagacattcgcaatcaatcagctcgaaatgtggatgaagctgagcttgttgggttttctgactccaagaaaaggctg 40878
cttgaaatgatcgataccaatgctaatgatggtccggccaaggtaatctgtgttgttgggatgggtggtttaggcaagac 40958
agctctttcgaggaagatctttgaaagcgaagaagacattaggaagaacttcccttgcaatgcttggattacagtgtcac 41038
aatcatttcacaggattgagctacttaaagatatgatacgccaacttcttggccccagttctctggatcaactcttgcaa 41118
gaattgcaagggaaggtggtggtgcaagtacatcatctttctgagtacctgatagaagagctcaaggagaagaggtactt 41198
tgttgttctagatgatctatggattttacatgattggaattggataaatgaaattgcatttcctaagaacaataagaagg 41278
gcagtcaaatagtaataaccactcggaatgttgatcttgcggagaagtgtgccacagcctcactggtgtaccaccttgat 41358
ttcttgcagatgaacgatgccataacattgctactgagaaaaacaaataaaaatcatgaagacatggaatcaaataaaaa 41438
tatgcaaaagatggttgaacgaattgtaaataaatgtggtcgtctaccattagcaatacttacaataggagctgtgcttg 41518
caactaaacaggtgtcagaatgggagaaattctatgaacaccttccttcagaactagaaataaacccaagcctggaagct 41598
ttgaggagaatggtgaccctaggttacaaccacctaccatcccatctgaaaccatgctttttgtatctaagtatctttcc 41678
tgaggattttgaaatcaaaaggaatcgtctagtaggtagatggatagcagaagggtttgttagaccaaaggttgggatga 41758
cgactaaggatgtcggagaaagttactttaatgagctaatcaaccgaagtatgattcaacgatcaagagtgggcatagca 41838
ggaaaaattaagacttgtcgaattcatgatatcatccgtgatatcacagtttcaatctcgagacaggaaaattttgtatt 41918
gttaccaatgggagatggctctgatttagttcaggaaaacactcgccacatagcattccatgggagtatgtcctgcaaaa 41998
ctggattggattggagcattattcgatcattagctatttttggtgacagacccaagagtctagcacatgcagtttgtcca 42078
gatcaattgaggatgttacgggtcttggatcttgaagatgtgacattcttaatcactcaaaaagatttcgaccgtattgc 42158
attgttgtgccacttgaaatacttgagtattggatatttgtcatccatatattcacttcccagatccattggtaaactac 42238
agggcctacaaactttgaacatgccgagcacatacattgcagcactaccaagtgagatcagtaaactccaatgtctgcat 42318
actcttcgttgtagtagaaagtttgtttctgacaactttagtctagaccacccaatgaagtgcataactaacacaatatg 42398
cctgcctaaagtattcacacctttagttagtcgcgatgatcgtgcaatacaaattgctgaattgcacatggccaccaaaa 42478
gttgctggtataaatcattcggtgtgaaggtacccaaaggaataggtaagttgcgagacttacaggttctagagtatgta 42558
gatatcaggcggaccagtagtagagcaatcaaagagctggggcagttaagcaagctgaggaaattaggtgtgatgacaaa 42638
tggctcgacaaaggaaaaatgtaagatactttgtgcagccattgagaagctctcttccctccaatatctctatgtgaatg 42718
ctgcaggatactttgtgcctagattctatttcctctcctcctcccctactgaggacactcgtgttgtatggaagtcttga 42798
agagatgcctaactggattgagcagctcactcacctgaagaagatctacttattgaggagcaaactaaaggaaggtaaaa 42878
ccatgctgatacttggggcattgcccaacctcatggtccttgatctttatcggaaagcttaccttggggagaagctagta 42958
ttcaaaacaggagcattcccaaatcttagaacactttcgatttacgatttggatcagctaagagagattagatttgagga 43038
cggcagctcgccccagttggaaaagatagaaatcagattctgcaggttggaatcagggattattggtattatccaccttc 43118
caaggctcaaggagatttcacttggatacgaaagtaaagtggctgggcttgctcagctggagggagaagtgcgcacacac 43198
ccaaatcaccccgtgctgcgaatgagggaggaccgaagtgatcacgaccttgcttgtgacgccgaaggatcccctgttga 43278
agtggaagcaacggatcccctcccagagcaggagggagagagctcgcaggtgatcacgttgacgtcgaacaacaggtcag 43358
tcactccctacatggcagcttaattaacttgtttcaaattatctttttgttcagtattagcgatcgggtgatatcgatga 43438
tttcaactcaccctttcatctctctggttttcttaacctaacagcgaagagataagcacagctcaagctggttttatcaa 43518
gtgatgatctcctcctccattggcatctccggtcgtccctgcttctgcggctgcgcacacctcgctgttccgaggagggg 43598
tgctgatctaaggaggcttccactttcttcaattgcgtctcacgctctcgattcttccctctcgggtatgaattgtttca 43678
atctgaccttttctcgtgatatgctactggttccagcatgagcatttgaaccagcagcttagaattatcgtttgatcagg 43758
tgttttttttatccattcttacctgggaactctacttttccaactcattcagaacagaaaccatgtttattacactatag 43838
aggggaacacgagttgtggttttgttatttcctttttggtgtgcacaccaggtgtttgctagaatgtctgaaagagcttg 43918
tgtatacttggttggctcaacattatgtgttaggtcatattttatatagttgctgacctatcacatattgcaggagtaaa 43998
atttttgctatgtttattgttccttgctgttgattttccttttcgtaaagaatattagctttttagataacgaaaggaat 44078
attatgtatcctgcaaaaaggtttgatttggtggtcactggctagtagaaaagcaacgagagatcagacaaaacaaagtt 44158
atggctacaatagctcattgcaatttatttgctgaacatgtgaaaaattaataattgtcgcatgtcatgttgctttggca 44238
ttctgcctgcaggtggttagttgtttcatatccattgtcttgtctaccttttcctactaaactagctaggagtagaaaaa 44318
gtaattgttggttccaagttcaaaagataggcagatgttagctaaggaacttgcagttcttgatctccttcagagcttct 44398
cacctcacatatatggatctctgtttatcctaaagcaacatttttttaatctgctagtttcaaattgatcatctttgtta 44478
ttcttcatttattttatttttctccctaagagagtttgctagggtggagatgtggacaatcgaatgcagctatgctgttc 44558
aaaccactgtcaaaagatgcaggacaggatgggcagatgcttgttcagtgttcttgttcaaatgaaacctatactattct 44638
ggaagaggttggttaatttactttgtacataacccttaccttgttaattatcctcttgcaaaatgcatggattggtgaaa 44718
tcatgttttttgtcacgcagaattgttttctgaagctcaaagttgaagctgcaatcttcactgtttctggcagaaccaaa 44798
ttggtggcttggtgctgcatacctgcagagaacgcaacggccactgcttgtagccttgaggatgctacattattgatcat 44878
cttactaaggcccccgatcaaactttgaatgtaattagatctgcccaggttctcaaaatttcttggctccgccgctgctt 44958
gaatcgatatgtcatatgctgcaaattaagtgaattggagatgtacatgcgctcgctatattgtaattgtggggtagcaa 45038
ctttatctcgggtacaaccatggaacaagggtgatgaatcaaactatttgctgtcatttgaagagctccagcagacatat 45118
ttcatgaaattgtactaattcttatttgtgtacaattgattatctcattacatcttgcattctgttagacatatgtattt 45198
ccatacattgtttacacctgctatgactgattgaggatatggcaaagcttaaaagatgatgttaacatggtcacatggag 45278
atgaaggaacatgcatatacagggcactccattttctgttttctctcaatcttatgtagagttaatatatggacagtagc 45358
agtagttacatctttctgtcaactaggcatactacccacgtgttgctcctggtcttttcggcatggtctatattaagatg 45438
taaacttatttattaaatcaattcaatcgtgtcgcattggttcatttatttacacttgttatgatatgatccctgttgta 45518
aaatataagaacttttacgattctaacactatttataatatttatatgagcaattttacagtacttaaggaggtaccatg 45598
aggtttaaaaatttagtacctcctagtacttaggtatcaagaggtaccaaatataaaatagaaaaagtggtagctcatca 45678
gatactataaaattgctcagattacaatagtacttgtctcactagttattttctattcagttcttttgtcttacccccac 45758
atacctttccacactcatccatttctctggtgaataactaaaaatgtttatatttcagagcggaggtagcaagaaaatac 45838
aaataactagagtaaaggctgttgaatagtctcacattagttgtgaaagggcaaatggcacaatatataagtggggtagc 45918
cccacaccttattacctagcttttgtgtgggaaaagaccttttacgatcttacaattggtatcagaggctggctaattca 45998
acatctttgacccaagggcactactagaaaaagggttttcgctgtcggccagaggtgctgccagcaacaaaaggccagag 46078
aaaataatacactttcactaatggtcgatcgacctccagcgaaaatcaattttcgttggtggaggctcattttcactggc 46158
agtggggcggccaccagcaaaaagttggtttcactggtgttggtatttcttacgaccacagataaatccgcaagcgcacg 46238
gaataccgcagtagcacttcacattcgggtgaccccaaaaggatatcgaactcagggtacgtgtataatctacctaagac 46318
taagacaatctaaggacaacaactgatggtaggcaggctagactagagaggactttctatgtgttaattatctacgctaa 46398
gcaaaggtaatttcctatctgttgcttcaggcaccaacccctgctgatctgccagcgtggttccggctatagctctatga 46478
tgtatccgaacgttgaggattactaagacgggattcagggctgtcaccacctgctatctacctctgacaaatccgtggga 46558
tacagaacaaacaaaggtaatctctaacctagacaccacgtctaagttattaattactactctaatacttgcgtaggaac 46638
ttcctttaatatccagagtcttagtactagagcacttagtatgaatactaaacaataaacccgcaaagatagaaatctat 46718
cttacttagactaagtaattaaataacataagaaaggaacacgaatgcttactttatagaagaagttctccgaacccgga 46798
gtagagtgtaccgacagctccggtactcctccagaattcctcctagaaggctacactcgtcaagaaagaagttggagaga 46878
gcgccaaacttccgggcacacctccagagcttcccctcactctctaccttattctaatgtacacaagatagaatagagcc 46958
tcttgcaattcagctcaaaggtgtgtgttgtgaatgagagtgaggagcactatttatagcctccatatgacggttacagg 47038
tatgggaaatgaccaaactaccctctaaccatcattgggatgaaatcaagaccgtccaggtgaaatatggcgatcaacgg 47118
cctccagatgggttcggccgaacccaggacggctccgttcaacccggttttcctcatggacactaattttgaggccctca 47198
gcccattggcatggtggattgggccatttggaggtgtttaattgacttatggggcctccaatccgtgtaaacgtgtcctg 47278
aatgagcggaggtgcattttatcgcatgacggatgtgttttctctttaaaatatctgcattcacatatttcaccaacgct 47358
agtggaaatgatactatgttttatttcattatatgcaggtatggacggtcaaatgttatgatttaaggaccgtcaacagc 47438
tggcaacaagtaatgagacccgccagtgaaaataattttggagaaaacaaatataaaaatcagatgtgggaactgtcgac 47518
ggcatgatcagttggtgagggtcaacagcgtagtgttgttggtgtcgccgccaccctctcccccaccatccggtagtttc 47598
cgctgtcatccttcaccaccgtgttgtcattgttattctcgttcttgctgttgtccctatagtggccctcatcgggggac 47678
gtgagcaacgaggtggattgtgagagggaggcgagcgtgaacgcttcgagcagcgaggcggaccacgagagggcagggac 47758
agcatggccattgccagatccactgccaccacccctcatcttcgacaccatgtaagcggacccggtggtcctaagcgttg 47838
ccgccgccgccggatctaccacctccaccgccaccacgcctctccttcgccaccaccagatccatcccctccactatcac 47918
cgcttgtctccttcgacgctgtgaaggaagatctgccaaggggaagtcactgttgtcgttgttttctgcttgtggccgat 47998
ggcgaggtcgaggtcactgctaccgacaccgaaccgcaaccctagccgctggatccatccactacaccgtcgccgcccgt 48078
ctccttccccgccgccggatgctcgcatcgcgacccaccgctgctggatccgagccctcgtgacccgctgttgccggatc 48158
catgccccctcccctctggccaaatctaggagagggaggggagggttgtgtcgccgtcgccactccctgtagtgcggcca 48238
cctagtcgtcgaggccgtgcgcgttctgcactggaccacgccgctgcctctcctgcgcgctgccgccgagctctgccacg 48318
caatgccacctggccaagccgtgtcgctgctgagatagccgagaaatcgagagagagagagagagaggagatagatagat 48398
tgagagagaagttgtgccgctgctgagataccgagagagagagaagatatcgctgagagggaaccgagaaaaaaaaagag 48478
gaagtgaaaatttgacttccgcgcatgctcccacgatttttgctggcggtggcttaggtgggcagctagtgaaaattgat 48558
ttttgctggcagtggcttaggtgggcctctagcgaaaagggtgcagcttcgctggccggccgccagcaaaaatacctgtt 48638
ggtaccggattccaaaaatttgtgcaccgttacccacaagacaagatcctttatttctaaccatttcttaacccggccgc 48718
cagcaaagatccattttcgctggcagtcctttgccccacacccctcgttacattttctctggcggtgttcgtgtatggtc 48798
gccagagaaaaaagataggtggacgccatgaaaaattgattttgtagtagtagagacagtgtgtgaaagcctgatggacc 48878
gtgggtgcctgtgcggcctgtctacgtgagggactgtggggtatggcaaaggggcggtaaacggctgagggactgttgac 48958
ggctgttaagtacaaaattcttgccgtcaatacctgcataaaaccataaaagtaaaacaaccaacatagtagtatgattt 49038
atttctaactattttcacgagtgttggtgtatattcgtatgcaagtaatcaaccaccaaagttgggagagaatgatcgtg 49118
aagaatgcaaatatactttgggatgatggacccacatggttttgaactataacatataaaatgtgcaatcattttgagct 49198
aaaacatggtgtgccataaagtggggcccacatgtcagcatcctgaggaaggactaggacagcagagccggtcagggtat 49278
ctagcccaattgtatgatggttgtactcctcatcccaatgacggttgtggccgtttctaccggtggaaaccgtcataaag 49358
aggacacttgtcaagaggagagtgaagattccatttagatgcttagcatcttcacattcccaaggcatctttccacacta 49438
taaataaccctctcctctcccttattcacacacaccaaagcgtgagctgaattacaagattagggagagagtaaggtaga 49518
agaaatcggacgaattccggagttgtcggtaatctcctcttacttttgtatcttgttaattactctgctttaatagaaat 49598
atcattctgagtaattaagattttcttaatgagaattacttcttggttagttcctaattagcatacatgatcattgttca 49678
ctgtagtctattaaattatagttattgatttagtgtttgatcggcactagagtagttattaattgcgtagacgcggtgtc 49758
tgtgtaattaatttcctgttgttgttgtgtatcccatagtacgtttgaggtgggtgtagaggtgctgatagccctcaaga 49838
gcacttaagtcctccctgtccaggtacatagtagagcgatatccgggaacagcgggttgccagtacctgaagtattgcgt 49918
tagattaaaattaaactttccctagacattgtttgtcattaggatgtcctctgtcttctgcccacattagctttgtgtcc 49998
ttggatgaacctgacgaggagttaaactcacacgttcaatgcggaaatcgatacactggaatactcccgggtgaagtgct 50078
acatcggtatcttccgtgtgcttgccaattctatctgtgacgttaagaaataccaatagggacaacaaggtgtgaagggg 50158
gaaattattgaattgtcccacattggttattaaatggcaaatgacctaatatataaatgggggagcctctcaccttattg 50238
gttagcttttggagtgggaaaaccctttaagatcttacaaaggcatgcaatatttatgtggcataaaaaacaattttctt 50318
ttcctttttatcatttttattataacatgtggctgatttgatacatgatatgaaagtttggccattaattgttctatata 50398
gttaatgctagtacctactccctccgttgcagaatacaaacatttttggttggatatgtgtattaagaaattatgtagat 50478
ttaaattaggaatattatgattagttgaacagagaaggtatgtgagaaaattgaatggtagaggattgtgattggtcgga 50558
aagataatattggtggagaagttgttatattttaggacaaatcttaaatactagaaattgttatattttgggacgaaggg 50638
agtaaaatataccgacctaccactacgaatctgaatggaaggttactagagtttattcagattgattgtactaggttgta 50718
tctattctattactaggttgctatattttaggatagagagagtatgaaatgacttgttgaactaatagtggtgttaagag 50798
cctctttggggagcttctagcagttataatttctcccataatatatagatttccatacagtgtagattcttacctacatt 50878
ctaagaagctaggaaacctaggtttccagacacttagaagctggctaccaagtggtgcttctcaggatctgaagctcccg 50958
aaacaatagctttctcgacccaatggcttctaacagtatgtgtggcggtggcaatttcccaaaaccccatgacctctcgc 51038
gcatggaaatcagtcacattgacgagcaagctcatctctttaagtctcagttcgtttgggctcattatcttaaagaacca 51118
ttccaacttaactgggctccttaatcttaatgaaataaaagcttagcttggcttgactcgcgagctgatttgttaagctt 51198
ttgttaggcttgttaacatatttatatatcctttataattttaaatttaccattttatcatattaacatagtagtaacac 51278
taaatataaatagcttggataaaattgaaagactaaataatacaaagtatatgtagttatgatgatctcttgtactaaca 51358
agctaaatgagcaatctgtgagctgcctcattaagctcacaagctaaaagcctatctccgcttgactcataatgattaca 51438
agtaatctaaaaaaaaataattacaagttcgaacaaaactcactacaaaccaatccaaaccacaatctttgagctttctt 51518
tccgtccctagcagtgacgcttgtgttgagacgaatcaacatgacactataggtcacaccccttcctggcaccacctaat 51598
agtgacaccagagggagtgcaccgtaggagatcagtgtgaacctactcccacggaaccgctatgctggtaccagaaagct 51678
aggcggcagccggcgggcatggcggcgctgcggaaaacgcggaggcgagcgaatagcgatgacgtcgccccctggtgtgt 51758
cacgcaagttaccgcatcaagcacctgcgcgtagaatctcattagtgaatcggactttctcgttagcggaaagaatcaaa 51838
ttgctccctacgggtgcacatgatttacgtgggtgtgaaatgggaaagggcgttgcaaaagtccgtgcaatataaggatt 51918
gacggatgacaaaaacaatagttatgttttaagtaggtaaagataaatatagacagatgctccggggccattactgccgg 51998
taaacccaagccaaaattggaaatttttctaccaaaagatcttattagtgtcggttctaataacgaacctgcactgctga 52078
taagtgccttatcagtgccctttttttttacaaaccaacactgataagacccgtcatattaaaaccattttatttgttga 52158
attggcactgatactgccagttcttgctcctaggagcgcgcacgggctcaagtgaacgagtgacctctaccagtaccagt 52238
ttttgtctaactcgcacttaaggatcgtgttcaatcccgagatcttttaattgtttacacctagaggcatccgccacctt 52318
ttggtcgtcgccgccacctccattgtccttgttataaaatcagacccggtagcacatcgagaaataaacacagaagaaac 52398
gtcacaggagatccaaacacgatatcgccgctgaaccaagatattagtagaggtcctgaaccggctttcctccgtctgaa 52478
tcggcagtcgtagccactgaatcagctttctgttcctgaaaaccatcaacgaaggaggggcagatcgatcccctttctcc 52558
catcctcgctcctcgcgctcacacggcactcctcagcacccgccactgctgccgccaacacaggagccgccgcgcatcac 52638
tgctcctctgtgcccctgaggacgtcaccgccggtggccacttccgccgccgcctccttgcctcctgaacgctgccggtg 52718
agagtgccgccgctgtgggcttcccgccggtcgaggacgccgatgcccacgagtcgctccaacgcagaggaagtcgccgc 52798
cctgctgtcggaacccgccgctccaccacctggagccggcgccgccaagtcgtccgccgcctgcaccgctgtttgatccg 52878
ctcgacgccaaaaccatagtcgccagccgttaatagttcggcaaatttaggaggaagtttgaggctcttgtgtcgctctc 52958
acgcgggtggctcgggggcagaaccgccgctggaaaccgcgcggccggcccggaaggtagcggcggggaaaatgtgccgg 53038
cggtggagttttcttctcggcgtgggaggagaaacagcgcgcgtacggcgggaggcggcggctggcggggcggatccgcc 53118
tccggcaacattcgccatgcatcttcatttgtctccgcttgatgctgaccgaaggggtccctgacctaccatcaacacaa 53198
atgattgttttgtattcgtgccatgcatgacccggtccagtactgcctgcacgcggagagtgcaacgaacctcactaccc 53278
acctaataggactagtaaacgagaagtcaaggtattttattgttgctcatggctttgtggaccgaattgggatattggat 53358
tggtcaggtacctgagtgagtactacgctgttaatatagattgacatgaaatggaaaacagagtcctcggcgtctaaacc 53438
cacctaacccatgatagactgctggtaatttaatttcaaatgtcaggggaatatgattgtctgcatgatgcgcgagtgat 53518
ataaactccatgataagcatcctctctgctcagacctcagagagctcactgcaaaagctaccatttctgattcgatctgg 53598
tcgccaagttctctcccctgtgcagtctttggcgaaggtgaagaatccattcaatcgatggcggagacggtgctgagcat 53678
ggcgaggtcgctggtgggcagtgccatcagcaaggccacctctgcggcggcccatgaggcgagcctcctactcggcgtcc 53758
agaaggacatctggtgcgtactgcacgaaaacctcacccgtagtttccatcttcgcttccgttttattgattgccagttt 53838
caaccaaaatatgtcaggcaaaactattgctgaaatgcgcaaatatatcagggacttaatatatgtttttactttgccta 53918
tgggtgcgttcctttttcttgaaacggttgcaccccagcttttagtggaagcatatctctatctctactaatataaaaat 53998
taaagatatttttaccggtactttgatacgtcatccatgtatgagtctgttttaagttcgttcgattttggaaatacata 54078
tccatatttgaatcggtttttaagttcgttcccttttggaaatatagaaggagtcgaataagaaatatctttgaaaaaac 54158
tcgcatgctaacctgagatgatcggactcctaattgcaactcatgattttctaaaaatatatatatccaagcaaattacc 54238
acaacgaatttcaccttaattaaaccgtataacaataataagaaataaccttcatctgttgtaacgcatgggcatttttt 54318
ctagtaaattataaagttatcttagtagtgaatgggacaccaataatctttctaaaacatctctttaagtggaggacgca 54398
aatctttgcctttaatttagaaaaatatttcttcttgtcagttaagcaaagaaagtccctcggtggtcgaaattaaccgt 54478
tccataaatcatgtgtgctaaatccactgaactgaagtagaactgtttaaaatacgagaaattttttatgccttgagaga 54558
taccaagacgtacctgttgtcgaaagacaactgtttcatgtcgaaagacgtggttcactcagtgaattaatggaatagta 54638
aaagcaatatattaattggtaagaaattttttgggatctcccattatatcatcttaggaggctatttatagccttaacac 54718
aaactcatgcctcctagggtttaatttgtataggggaatttacatgggtacccttatgatagggactttagtgagggcac 54798
acaatatcatttgtgtttatgggctcttacgcgaactccttgggccggcccattactggtgaaaggcctccatggtgtct 54878
cccaagccctctgccgaagccacttctaacaagggcgaaaccctcctgcttcactctacagcctttgcccgaagtgatgg 54958
ctcgttagggtgatgggcttggtggcgaacccctcggtcttcatcatctaacttcgtcagctgcttcgccttatggcttc 55038
gcctcatggaatcagctaacctcaaggttctcgtggtttcagcaggcccggccgaaggccctcgtgatatgcctccaaca 55118
gtaccataaattttcatacctcctagtaccgtagataccaaattttatattagaaaatatattcggtacctcctcaaaga 55198
ttgaaaaatcactcttaaactacacacatgtccataaacttggaccaatcaatcatgtcagtgcgtgaacaagtaagata 55278
tgtaccgatatcctaacactactgggaacccatctttagtcccagtttgcaaccctctttagtcccggttgcaaaccgag 55358
acgcccaaagcacatctttagtcccgattggtatttgatttcgagtggttccatgccccacatcgagttgctctcgacat 55438
ccccaaatccccacatcaaattgacatcatatatcgaatgaacatattcaacaacaaaatcatccataaattcacaaaaa 55518
catcttaaaaaatctacaacaaaatcattcatccacaaattcttttctttattcacttctctccttgcttctcctcgggc 55598
aaggcggctggcaagcccggtcgagctcctcgtcgccgcctcgagggagggacaacagccacgtgagtagataaggacgc 55678
ggctctctccctccaccgtgcgcctctttttaatttttagtctcggctgctataaacaatggggactaaagtgatgatct 55758
ttaccaatcgggactaaagatcgttggccgcctataacaattgaaccgtggctaaaaataatctttagtcatggttggtg 55838
ttaccaaccgggaatataagattataaagtgtcgctgggctttttaaccggggctaaaggtattttatagtcccggtttc 55918
tatttgaaccgggacaaatgtgatttttgtcatccgggcaaagatcagttctccattagtgtaatctgggcaaattttat 55998
tacatgacacctgaaaattgtgtaatttgctcatagacatcgaaaaatgtgtcatggatgaatgatatttaaaagattga 56078
taatttgcaagagaatactttcaactcaaattggagggataaattgttaagaaagacatgaatgcccctatgaccacatg 56158
cttcaaacatgacgtcagagtaaaaattttgaaattatgcaactctaaaattaccatcatcccaagtacatcactacata 56238
cattatattcatgttcactccaacctccgttcaaaagcttttgaccctaggggtattctcgtatttttgaagaatttttc 56318
tcttcaaataagctgaaagcatcctccagcaaattatcagtttttaaagtgtcttttagccgtgacatgtttttacgatt 56398
actaccagcaaactacacaaattttgattgtcctgtagcaaaaatttgcccctaatctttgtataatgtagcattagaga 56478
ggtctaaagtgcatatgacatggttcgactgttgatgtggccaagaattttgcctttagaccctcgtgctatagtacgca 56558
ttcaagtagactctcaccactcccaaatatttttgtacatcacttaaaatacaaaatttctaaatgttgagaaatgcata 56638
tttgcttatttcgggtggtaagaatcggttttagaggcaaacagttgtgaagggacctaagtgtaaaattctcgccaccg 56718
tagtcaggcatgtcagcatttgagttactagctgactgcccatgcatgctttgcataggaattaaaaaaaaaactatcgt 56798
gcgctctaaagccccccaaaaacataataaaaatatcccattgtattcaatttcacattattttttgtacatgtatgtga 56878
tgctatggaattggtcccaaatgtcagtggtggtgggcagtgacagatttaccattcatcaccattgcagtcttttaaat 56958
aaccatctacagtttagacttgaaatgatacattaaaccaatattaaatacccatatgtgcttataagtttatgtaccta 57038
aatgacaccttcggttttaaattcaaggacgggtcatgtattttactcaaaaacttatgatgtctttaaagtatatattg 57118
tatgaaaatttagtcattttattatttttctaatataagattcggatttctactagagatatcctaaattactttgaaat 57198
gaatgattgcaggtatatcaaagatgagctgaaaacaatgcaagcgttcctacgagctgctgaagttatgaagaagaaag 57278
atgagctcttaaaggtttgggcagaacagatacgtgacttgtcctatgacattgaagattgccttgacgaattcaaggtc 57358
catattgagagccaaaatctgttttatcagatggtgaagctcagaaagcgccatctgatagctacccaaatccgtaacct 57438
caaatcaagagttgaagaagtgagtagcagaaactcacgctacaatttagtcaaacctatttcatccagcaatgaggatg 57518
acatggattgttacgcagaagacattcgtaatcagtcaactagcaatgtagatgaaactgagcttgtggggttctctgac 57598
tctaagataaggttgctcgaattaatcagtgccaatgtaataatggtccaaccaaagtaatatgtgtggttgggatgggt 57678
ggtttaggcaagacagctctttccaggaagatctttgaaagcaaagaggacatcggaaagaactttccatgtaatgcatg 57758
gatcactgtgtcacaatcatttaacaggattgagctactcaaagatatgatacggcaatttctaggttccaattcactgg 57838
atcaagtcttgcaagaattgcaggggaaaatggtggtgcaaatacctcatctttctgactacttgagaaaaaagctcaag 57918
gaaaagaggtattttgttgttcttgatgatctatggtctttagatgcatggaactggattaatgatattgcatttcctaa 57998
gaataacaataagggcagtcggattgtagtaaccacacgagatgttggtctagccgagaagtgtaccacaacttccctag 58078
tctaccatcttgaacacttgcagatgaatgatgccataactttgctactgagaaaaacaaatagaacacatgaggacatg 58158
ggaacaaacaaaaatatgcaaaaaatagttgaacaaattgtaaataagtgtggccgtctaccattagcgatacttacaat 58238
aggagctgtgttggcaactaaacaagttttagaatgggagaaattttataaacagcttccttcagaacttgaaagcaacc 58318
caagccttcaagctttgaggagaatggtgacccttggctacaaccacctgccatctcatctgaaatcatgctttttgtac 58398
ctaagtatcttccctgaggattttgagatcaaaaggagtcgcttggtagacagatggattgcagaggggtttgttagagc 58478
caaggttgggatgacgaccaaggatgttggggacagttactttaatgagctaatcaaccgaagtatgattcaaagatcaa 58558
gagtgggcatagaaggaaaaattaagagttgccgagtccatgatatcatgcgtgatatcacagtttcaatctctagagag 58638
gagaactttgtattcttaccagtgcatgatggctccaatctagcacaggaaaacactcgccacatagcattgcatgggag 58718
catgtcctgcaaaacaggattggattggagcattattcgatccttagctatttttggtgacagacccaataatctagcac 58798
acactatttgttcaaataaattcaggatgttacgggtgttggatcttgaagatgtgaagtttttaatcacacaaaaagat 58878
ttcaacaatatagcattgttgcgtcacttgaagtacttgagctttggaagaatattctcatcatgcatatatactcttcc 58958
cagatctattggtaaattacatggcttacagaccttgaatatgtcaagtacatacattgcaacactaccaactgagatca 59038
gtaaactccaatgtctgcgcactcttcgttgtacaagggtatctaataataacaattttagtataaatcacccggtgaag 59118
tgcttaactaacacaatgtgtctgcctaatatattcacaccttcagttagtagcgacaatcgtgccaaacaaattgctga 59198
attgcacatggccaccaaaagttgttggtctgaatcatacagtgtgaaggtacccaaaggtataggcaagttgggagaat 59278
tacagatactagagcatgtggatatcaggaggactagtactagtgcaatccaagaattggcgcaactaagcaagctgact 59358
aaattaagtgtgacaacaaagggatccacagaggaaaaatgtaagatactctatagagccatccaaaggctctgttccct 59438
gcaatctctccgtgtggatgctgaggggtcctcaggaaatggaacacttaaatgtctagattctatttcctatcctcctc 59518
tgttactaaagacactcaagttgtatggagatcttgaagagatgcccaactggattgagcagctctcgcacctcatgaag 59598
ttctacttattagggagtaaactaaaggaaggaaaaaccatgctgatacttggggcattgcccaacctcatgctgctttg 59678
tctttcacttgatgcttacctcggggagaatctagtattcagaacaggagcattccaaaagctcaggacactttggtttg 59758
acaagctggatcagctaagagagattaggtttgagaacgacagctcgcccctattggaaaagataggaatccgatactgc 59838
aggttggaaatagggattattggtatctctaaccttatgaggctaaaggaaattacacttggatacagagttaaagttgg 59918
ttatcttggtcagttggaaagagaagttggcacacacccaaatcgccccgtgctacgtatggaggaggaccgaagctgtc 59998
acgacctgagaagggatggcaaaggatcagctgtagaaatggaagcaacggagcccctccctgagcccgagagctagaac 60078
tcacaggtgagcacgttgacaagccaattccctgcattgcagctctactagatttctcttctgttcagtattagccatca 60158
gctgtgatgattccaaccaacctttcatctctcctgttttattaacataacaatgatccaataatggaatgctttctaaa 60238
aggaaatctatcaccggccaatgatgtggataatcctagaaagacttcgagagaagagatgaaaatgttacaaatgttaa 60318
tcaccactgatacttcgttgatgtcaacaaaagaggtaaatttgagtgagctgccttacgaactatctgagaggcatcct 60398
gcttttcctttggtttaccaactcctgaaactagtattggttttgcccgttgcaaccgcatcagtggagcgatgcttttc 60478
agcaatgaaaatcatgaagacaatatactacagaacagataagtgataaacttatgaatgattgtattgtatgcttcatg 60558
agccatgttttctagctacaattcgaaatgatgacatgaaagctctccttgatcaaaaggagggaacttgcaaagagaag 60638
atgtaataggtaattaactattccaacagaggtgttgtatatttatatcatacagtgctcttaatttatttagcctatta 60718
taatgcatgatattagtctattaaagcaaacagtttatacttattctaccggttttgttagcatatatttaatattcgta 60798
gtgactagtgacctgaccccagcgaccattaattttttccttcttccgccctggagaagagctaggcacagctgtagctg 60878
gctgatggtctcctcctcctctccagcgccatcctctccttgcacactggctctccgtctccgggcgtccctcctgcgtg 60958
cacctcaccgctccgaggacatgatgatcaacgaggcttcctgcttccagttgctaaatataatatgtgcctaacactct 61038
cagttgtttccatcccagggtatgaaattgatctatcagattcgctccttgccacaaggtgcaaacatttgatgcttagt 61118
ttgatcagggtgttctgtttcagggttgtggtttgcacaattactcccactttgtgcttagatatgcttaggctgagttt 61198
gaggagaaggggacatagaagattgggaagttacgcaaaatgaggtgagctattacactagtacagaacctttcatctgt 61278
gaacatgcttggaattagcggccgtcacaagaaattcatctcaatcgggtcgacaaagcgccggattgagataactcagg 61358
cccgtcacggatcacacatatcagtgacgggccgtagtttagacccgattgagataacattccatatctcgatcccgtca 61438
cggatgacacatatcagtgacgggccgtagtttaggcccaattgagataacattccatagatgacacatatcagtgacgg 61518
gcctaaactacagcccgtcactgatatgtgtcatctgtgacgggcctgagttatattcccatctaattaacatgtctatg 61598
cgaccatatctcaacaactaggcccgtcacagatgactcatctgtgacgggctctatactaatgcccgattgagatgact 61678
tggtattctttttttccatttttcatatgaaatatttatatctgtgagttgacatgtagcaaaataattaactgaagtat 61758
aataattcattttcttagtcaaaataatttttcgggtagtaaatgatttcaaatggaaaaattgtcaacaacaaagttgt 61838
ataacttatcaatatttacaatttttattttggtcatttttctatataactttttttaacagtttgaatttgaaattgaa 61918
aatataacaacttcaaaaaatattttcaaataataaataatttcaaatggaaaagtcatcaacaacaaagttgtataact 61998
catcaagacctataacttttattttggtcatttttctatatcactttttttgaacagtttgaatttgaatataaaaatat 62078
gacaacttcaaaacaatattttcaaatactaaatgatttcaactaaaaacgttatcaacaacaaagttgtataactcatc 62158
aaaatctataacttttattttgatcatttttctatataacttttttttgaacagtttaaatttaaatttgaaaacatgac 62238
aacttcaaacaacatttttaaatactaaatgatttcaactaaaaaagtcatcaataacaaagttgtataactcatcaata 62318
tctataacttttgcttttgtcatttcttcatccgacgaagtattagtaacattgttcacaaaattgacatatctgtgtta 62398
tagtttataaaaccagacgagatatgtgaattttgtgaacaatgttactatcactttgtcagataaagaaatgaccaaaa 62478
taaaagttatagatcttgatgagttatacaactttgttgttgatgactttttcaattgaaatcatttagtatttgaaatt 62558
gttgtttgaagttgtcatattttcaaattcaaattcaaactgttcaataaatgttatatagagaaatgaccaaaataaaa 62638
gttgtaaatcttgataagttatacaactttgttgttgatgacttttccatttgaaattatttagtatttgaaaatattgt 62718
ttgaagttattatattttcaaattcaaattcaaactgttcaaaaaatgttatatataaaaatgatcaaaataaaagttat 62798
agattttgattagttttattagttatataactttgttgttgacaatttttccatttgaaatcatttactacccaaaaaat 62878
tatttgaatttcttatattttaaaactcaaaatttatatagttcaatcaaacccggatggagaaatgacaaaaataaaat 62958
tggtagatctcaatgagttctacaactttgtttttgatagttttttcacatgagttcatttagtatcataaaattcaatt 63038
caaaatttattttcgactagaagtctgacttgcatcctcatcatgttaatacttactaaatatatatttgatccaattaa 63118
ctcggttttgctggtgaaatatgtgaatggatgtgaaatatgtaatcaaaatggatgttatatgaatgtggttgtgttaa 63198
atatattaatggttgtgtatgtgtacaacttcttctctggaaaccgatccccaaagaactctgaggttaagcatgcttag 63278
ggtggagaaatttcgggatgggtgaccgaccggttgcataaaagaccggcccgcgggcgtgcctagcagaggcctgaggg 63358
tagccaaaaaataaaatttattttttttattataatcatccgtgacgggctataatttaggcccgattgagatgagagtc 63438
atccgttacgggctatagtttacgcccgattgatatgagagtcatccatgacgggctatgtttaggcctattgagatgag 63518
agtcatccgtgacgggctataatgttggcctgattgagatgtgtgacatctgtgacggccgaatatccgattgagatatt 63598
ttctcacatcaggcttagtgacgggggtgctacccgatagagatgaggctttaggctcgttactgaccgaacctgttcta 63678
gtgttagtgcatgattaattgagtattaattattttaaatttcatcaatgcattaatattatttttaaagcaactttcat 63758
atagaaaatttttgcaaaaaactcaccgtttagtagtttgggaagcatgcgtgcaaaaaacgaaagcaattttcaatcct 63838
tgaaaccactttttctattgtaaatttgatacctcataaggtaccaaattttatattaaatttttggtacctcataatac 63918
ctcctcaattactatagaattgctcaagtttttatttctgttgacaaatgcaccagatgtttgttcagtcactactacat 63998
gaatggtttttccgtgcgggtggcatgatttttgcgtgcggggattccacccgtctggaaggtcgcatttgcaaaaatcc 64078
ccatttttttgtgcgggtgacgcacccgcacgcgaaaatcgattttcgcatgcggtcgcttaggccgtccgcatgggaaa 64158
ataaaaaaccaaaacaaaaaaaaaatcaacatccacccacaaaccctaacgtagccaccgtcgccgccgccaccaccacc 64238
gtcgtcgctgccttgcctgaggcccgtcgtcgatgtcgctgccaccaccgtcgtcgtcgccggtgaccaccgccgtcgtc 64318
cccgcggtcgccggcgaaggatccggcctccccgtggcggcaggcgacggatccggcctccccgcggcggcaggcgacgg 64398
atccggcctccccgcggcggcaggcgacggattcggcctccccgcggcggccggcgacggatccggccttcccacggtgg 64478
ccggcgacggatccggcctcccacgcggcggccggagacggatctgctccgccgccgccgcgtccatcgatgccgtccgc 64558
tgatgccgcgcgccgatgcagccagggtgcccgccgatgccgccaccgccgcgcccgccgatgccgccaccaccatcgat 64638
gccgccgtctccgcctcccttatcgagggcgccggcgtcgctgaggaggagggagagggagatgggaggatggggaggag 64718
gagagggagagggagagggaggatggatggggaggtggtgggaagttaatggcagcgcgcgcccgtgacgagagaggata 64798
agggtgagcgcgtggggtgggctattttcgtgttcatgcgggcctgttaagtgacctgcacacaaaaatagattttccta 64878
tgcgggcgatgctctgatgcccgtttttcatttttctgtgcggttgcactaatgggccgcctggaaacaaattagtgtgg 64958
tgtagtgtagtagtgagtattcttgttcgaaggaagaaaaaaaaatcttgatggtaggtcatttcattttaacccttacc 65038
cttatagattgctttgtcaaaactacaatttggagccccatgcaaaaatgcatgaatatgctgatattgtttctctgcca 65118
tgcagaatgcatatggcttgctgaagctcaatggttgaagctacaatcttggctatttctggcagaaatccaaattggtg 65198
gcttggagctgtgttgtatgccatcagagatcgcaacttttgtgcccatgaagacgctaattcatatatacttgaatcac 65278
gtgtcgctaataagcattatgatcaatctcgctgcagctagagttcaccatcattctcaactttgcgaatgctctggtgg 65358
tcacatggatcgaaatgtcgtatgctgcaaagaagaggagaactaaatgcattccaatgttcctggcaatattgtaatta 65438
gtaaacgtggaggagcgatactaattatctcggggatgtggtaaactgtttgctgtcatttcaagagctaggcagatcat 65518
tttagatcagctacttgtttgtaattcacgagtgcagaatgagtaattcgtacttctgtgcattgtatttatttccctac 65598
atctttttgctgtgttggaaataagagtgttttttgcacgtacggtacactggtgctccaataatctgtgttttgtctaa 65678
ttcttagggagataaacatggaatactatagcagcgtacttatgcaacaccgctacgttagtgcactggtgcagcctttt 65758
ctgttgttatgtaattatcttctgagatgttctatatatatatttcaagtttctctctattgcaattttgtgatcgcagt 65838
ttcttcttttcagccggatatgaagattgctgtgatgctgctgctatagcttgtactcactgattcctcactctttctac 65918
ttcatctttcttgttcagtttttattttattttttttagtgtgtgaccacacatttgaattgaatatggtctgtgtattg 65998
taagcgtacagttaattgggagtgtgcatctattcttaagtgtgctcggcacttttgaacttagatgggaattattggaa 66078
tgtgcaactatattcgtttacaacacttacatatactattcgtactcagtcgtgtgctgctatttaaacttgaagaagtt 66158
cttttttttggataacaggtatacaacataattgaagtatggtgcaacacaataaaaccattaacgtaagtccataagca 66238
aacaatttaaaagttatgggtgatatgtattttgaaagagatgaaatgtattagcctatttaagtcatcttcaaattgca 66318
acgatccatgttacaatagatttgcaggcttgctgccttccgggaaccggacaaaactaacatgtgggggtgagctggag 66398
cttgactttgatgttgttgtcaaagaccggaatggtttggtcatcccatctttactcgtgttaattagtttagtaagtat 66478
tatgttgtagcgtgggcatcttattatcgatctcaatgagtattacttaattttatatttattagttacctcacaggggc 66558
tgtactcatcttaagtcttacatcttccgtttcacaatgtaagtcattttagcatttcccatattcatattgatgttaat 66638
gaatctagacatgaatctagatagatatatatgtctagattcattaatatcaatataaatgtagaaaatactagaatgac 66718
ttatattgtgaaacggaaaaaataccatccatttcaacccccaatttcaggcttagtttgatttaaattatcgagcggat 66798
tgttgtgacagattttctgcaatagggaaaatttaaatattttgagaaatcagcttaataaatagcttaaaataatctta 66878
gaaatgcagtagaagaattacttttttaataacgtatatttccatgtgtggagaaaataatccactaaataattaatctg 66958
aaaaaactgagcccaacgacctgttcatatgcctttgtccggggtaaatatatgatgggaacaaagagtatagaagttcc 67038
ttaaaaaatttggaaaccaattaaactcacatatagataaggtgttatattggacgaacctgcaaagcctaaagataaac 67118
tcaaatttgtctgtggaaaaactacaaattggacgcccaatttttttctaaaagatcgagcgttgtttcaccaccttatg 67198
taaaaaaatgtttcagtgcctaacaaaatgatgtttcggctacagaaatgatgaaacacaattaaatcactcttggaaca 67278
tttttctacaacatataaaacatcggtttcaaaggtgttgaacaacagcaaatctgaattcatcgaagaactcactacaa 67358
cattttgattgaataaattaaacatttggatacaaagctaaaacatgacaaatccaaaaaatataatgaaacaccaccga 67438
gaactgacagcaacatcttgattaatctaatgaaacatttagatacatatgctgaaacatcgaaaatccaaaaaaaaaaa 67518
atcaggctagatctacctcttcctccactttggtgctatcctccggtcttcgttggggccgtcgatgttgcgtgagaaga 67598
ggatgtggccgtcaaggatgtcgcggcggcgccaggcattcaggacgacgagaaaaaatggtcggtgaagtcgatgacgt 67678
cgtaggcgatggcatagcatgggtagtgcgcctccacggggatgaggctgctgtcgacgtcgaggaaaccgaaaagctgc 67758
ggcggatgttgacggcgtggatccggtggaggaggtggcacaagcgaggttggcggaagagagagtcggcgtgggcgaag 67838
gagcacgggggagaggaggtggcgcagacgagagaggcaggggagaggagatgatgcggaatgaggcgatgcgaaagaag 67918
cccgatacttttgttcgacgtcggtcgcccgaggcagaacattatcgatatgagttaactaaaattgcaacaagtctacg 67998
agagcttgtttgaggagcttatagcagctttttctctccgaatttccagttccataaatcatgtaaattctcaccaataa 68078
tctatagttatagtagaatttacaacatcaaaataatcaagtatatataatctataagtaaaatttatagctaagaaatc 68158
tagctttccgatattctcgagaactgactagcaaccttttttataatcttaaaaatcctctagacatgacctaaaattgt 68238
atggaatgtaaaaaaatacgggattttcacatgttcaattcccctaaaaatgttgggcgcatgaccaaattgcaaaatgt 68318
accaggcccgagatcgatcgatctgaacggacgcacgaaggaagaaaaagtgtaggagttgcggcacatcaccgccgccg 68398
ctcctacaccgtccgccatagcgattcccttccatctcgcagccatccaaagtgagtccttcttcgccgtctccgatctg 68478
gaacttcaccgccgccgccgccgccgcatctcctccttcctctcttggcaactgtcggcggcagccaacggctctcttcc 68558
ccccttgttcctcccgctatcgcgagtcaacgggatgctcgattcgttacattttttttaagtactagtattgctaattt 68638
gttggagcaagctaactgcattgatcgctcacgtggtactgcttcgatcttcacaatcacccgataaaaatagttgcgac 68718
agagaaaaaaaaaaagaaagtgtgggacggaagggaaaggggggaattcgctataaaagcactcggaattggcctgctcg 68798
atgcgccgaggctgactgatctcctgcagttccaagactgaacagcgagatctctgcaggtatgtgcccgttagctgact 68878
ccacgagttctttttttcctggttcaatgtttccactgctctctctctctctcttttaaaattcggcaagagctgaacac 68958
ttcggctgtgaatctatgatgtcttcagcagatctgggtgcagtgatttgattcgcaggtgtaagcttgattagccatta 69038
cttgaggagttaggtttcatgtttggataataatcaggcctacttttggtactgtcgtactaaggtgattattgccgcgt 69118
tcccccccccccccccccttttttttttcatttacatttgaactttggcatgtgtattggatcatgcttatggttcaaaa 69198
caattacggtactaaagtttacttgcattagtattaacttttacagtatatatgtagttatgtacaaactacaaacaatc 69278
gcatgcttgttaactgtaattaggctttgccagcatctacgaattttctagctttgtaattattttgagacaaattttga 69358
atgataaaagttgctatattttgggacggaagggtagatatccacgaggtgacttaactctttccgctggcttttggacg 69438
tgctacattctgataaaaataaccgcatctagtcactctgtacatattccaaccttgcaaggaaatagatactacctccg 69518
tttcaggttataagactttctagcattgtccacattcatatagatattaatgaatttaggcatacatatatgtctagatt 69598
cattaatatatatatgaatgtgggcaatgctagaaagtcttttaatattaaacggaggaagtacaatttaacggaatagg 69678
cttcgattctgagatccattgttctaacaggactgatctggtggtaatggcggagacggtgttgagcatggcaaggtcgc 69758
tggtggggaatgccatcacgaaggccggagaggcagctgctgcagagataagcttactgattggtgtgaacaaggagatc 68838
tggtaggttttctttaattacttacgcaaacaaaatattggtttaggaaaacgttaaccctaaactttccaaactggtta 69918
ttttcaaacacccacccccaccccccaaaaaaagaaacaaaactcctggtaccaagtggtaaaaccattttaagtgatta 69998
ttcgaattgattttcatactttaggtgatagaataaatttaagccatgtaatgtcctaaaattgtcctcaaagacgagct 70078
gaaaacgatgcaagcattcctgatgacagctgaagagatggagaaaaaacccaggctgttgaaagcgtgggtggagcaag 70158
taagggatttatcttttgacattgaagattgcctcgctgagtttatggttcacgtggggagcaaaagcttgtcacaacag 70238
ttgatgaagctcaaacatcgccatcgcattgccatccagattcgtgacctcaaatcaagagttgaagaagtgagcgatag 70318
gaattcacggtactcattaatcagccctaacaccgatgagcatgacaccttgagggatgaatttcgctactggtcagcta 70398
agaacattgatgaggctgaacttgtgggttttgatgatgcaaaggaaagtatacttaatttgatcgatgtccatgctaac 70478
catggtcttgctaaagtgatctttgtagttggcatgggcggtctagggaagacaagtcttgttaaaaaggtttaccatag 70558
tattaatattgttaataatttctcatgccgtgcttgggtcactgtgtcacagtcatttgtcaggacagagctcctgagag 70638
gactaatcaagcaacttttgggtggtgattcggaaaatgaacacttcaaaggtcttcagagcatgcaaaggaatgagaaa 70718
gtggaagacctcgtggaagacttgaagcaaggtctaaaagagaaaaggtactttgttgttctagatgacatgtggagcat 70798
<210>2
<211>19
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(19)
＜223〉marker
<400>2
gggaggaggaatggtagga
<210>3
<211>19
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(19)
＜223〉marker
<400>3
cccaagacaagcagcctaa
<210>4
<211>19
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(19)
＜223〉marker
<400>4
cagtgaaacgaacgctatg
<210>5
<211>20
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(20)
＜223〉marker
<400>5
caacatcatcccttcctatt
<210>6
<211>18
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(18)
＜223〉marker
<400>6
aaggttctcgtggtttca
<210>7
<211>19
<212>DNA
＜213〉artificial sequence
<220>
<221>misc_feature
<222>(1)...(19)
＜223〉marker
<400>7
ctgctataaacaatgggga

Claims (6)

1, a kind of molecule marker of broad-spectrum rice-blast resistant gene of paddy rice is characterized in that described molecule marker length is 10～30 Nucleotide, and wherein the sequence shown in the 1-70000 position is identical among the sequence of molecule marker and the SEQ ID No:1.
2, the molecule marker of broad-spectrum rice-blast resistant gene of paddy rice according to claim 1 is characterized in that described molecule marker length is 15～25 Nucleotide.
3, the molecule marker of broad-spectrum rice-blast resistant gene of paddy rice according to claim 1 and 2 is characterized in that described molecule marker is selected from: SEQ ID No:2,3,4,5,6,7.
4. the kind with disease-resistant site of the marker assisted selection exploitation of deriving of a molecule marker as claimed in claim 1 design.
5. the new disease-resistant gene or the site that obtain of the labeled derivative mark of deriving of a molecule marker as claimed in claim 1 design.
6. the disease-resistant variety identified of the labeled derivative of deriving of a molecule marker as claimed in claim 1 design.
CNB2006100040589A 2006-01-26 2006-01-26 Molecule identification and transfer technology for broad-spectrum rice-blast resistant gene of paddy rice Expired - Fee Related CN100410390C (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
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CN101643790B (en) * 2009-09-07 2012-03-07 中国水稻研究所 Specific molecular marker of rice blast resistant gene Pi 25 for rice and special primer thereof
CN104404152A (en) * 2014-12-03 2015-03-11 云南农业大学 Genotype-expression molecular marker for detecting basic resistance of rice pyricularia grisea and application
CN104611332A (en) * 2015-01-27 2015-05-13 湖北省农业科学院粮食作物研究所 Molecular marker primers for rice blast-resistant gene Piz-t specific SNP and application
CN106929585A (en) * 2016-12-29 2017-07-07 深圳兴旺生物种业有限公司 The detection method of blast resistant gene Pigm and its application
CN107988218A (en) * 2016-10-25 2018-05-04 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012069 and its detection method
CN108018284A (en) * 2016-10-25 2018-05-11 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012070 and its detection method
CN109182342A (en) * 2018-08-20 2019-01-11 中国科学院遗传与发育生物学研究所农业资源研究中心 A kind of rice blast resistant gene Pisj and its application

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FR2708613B1 (en) * 1993-07-29 1996-04-05 Nat Inst Agrobiologica Nucleic acids markers for the rice rust resistance genes and rice rust resistance genes isolated by means of these markers.
CN1294278C (en) * 2003-10-15 2007-01-10 中国水稻研究所 Molecular marker SA7 for detecting rice blast resistant gene Pi25(t)
CN100345979C (en) * 2003-10-15 2007-10-31 中国水稻研究所 Molecular marker SK17 for detecting rice blast resistant gene Pi25(t)
CN1648253A (en) * 2005-01-07 2005-08-03 南京农业大学 Molecule label linked with rice anti-rice blast gene

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101643790B (en) * 2009-09-07 2012-03-07 中国水稻研究所 Specific molecular marker of rice blast resistant gene Pi 25 for rice and special primer thereof
CN104404152A (en) * 2014-12-03 2015-03-11 云南农业大学 Genotype-expression molecular marker for detecting basic resistance of rice pyricularia grisea and application
CN104404152B (en) * 2014-12-03 2019-05-03 云南农业大学 Detect genotypic expression molecular labeling and the application of rice blast fungus basal resistance
CN104611332A (en) * 2015-01-27 2015-05-13 湖北省农业科学院粮食作物研究所 Molecular marker primers for rice blast-resistant gene Piz-t specific SNP and application
CN104611332B (en) * 2015-01-27 2017-11-24 湖北省农业科学院粮食作物研究所 Rice blast resistant gene Piz t specificity SNP marker primers and application
CN107988218A (en) * 2016-10-25 2018-05-04 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012069 and its detection method
CN108018284A (en) * 2016-10-25 2018-05-11 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012070 and its detection method
CN108018284B (en) * 2016-10-25 2020-07-10 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012070 and detection method thereof
CN106929585A (en) * 2016-12-29 2017-07-07 深圳兴旺生物种业有限公司 The detection method of blast resistant gene Pigm and its application
CN106929585B (en) * 2016-12-29 2019-09-10 深圳兴旺生物种业有限公司 The detection method and its application of blast resistant gene Pigm
CN109182342A (en) * 2018-08-20 2019-01-11 中国科学院遗传与发育生物学研究所农业资源研究中心 A kind of rice blast resistant gene Pisj and its application
CN109182342B (en) * 2018-08-20 2021-08-20 中国科学院遗传与发育生物学研究所农业资源研究中心 Rice blast resistance gene Pisj of rice and application thereof

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Effective date of registration: 20081128

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Patentee after: China Rice Research Institute

Address before: 359, Stadium Road, Hangzhou, Zhejiang, zip code: 310006

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