CN1839998A - Chinese medicinal preparation for replenishing qi and blood and preparation method thereof - Google Patents
Chinese medicinal preparation for replenishing qi and blood and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese preparation for tonifying blood and process for preparation, wherein the preparation is mainly made from Chinese angelica root, prepared rehmannia root, root of herbaceous peony, chuanxiong rhizome and auxiliary materials. The preparation can be used for treating various gynaecologic diseases.
Description
Technical field: the present invention relates to Chinese medicine preparation of a kind of nourishing qi and blood and preparation method thereof, belong to the field of medicine technology.
Technical background: gynaecopathia such as menoxenia, dysmenorrhea, blood deficiency are weak etc. is to threaten the able-bodied common disease of current women, has brought great misery for numerous women.Commercially available blood-bonifying Chinese angelica essence is present nourishing qi and blood, treatment menoxenia, and puerperal, blood deficiency was weak, one of better medicine of anemia.The blood-bonifying Chinese angelica essence is made up of Radix Angelicae Sinensis, Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Rhizoma Chuanxiong, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali etc.Modern pharmacological research shows that ferulic acid, Radix Angelicae Sinensis volatile oil and Rhizoma Chuanxiong volatile oil all have the effect of anticoagulant, microcirculation improvement in the Radix Angelicae Sinensis, and the main effective ingredient in Radix Angelicae Sinensis and the Rhizoma Chuanxiong volatile oil is all Ligustilide.But we find: the character of Ligustilide and ferulic acid is all unstable, decomposes easily, and its content of prolonged placement can descend gradually in aqueous solution.In addition in the blood-bonifying Chinese angelica essence, some are insoluble in the composition of water such as the astragalus polysaccharides in the Radix Astragali, the catalpol in the Radix Rehmanniae Preparata or the like, in oral liquid content low and place a period of time after precipitate can appear, influence preparation stability; Secondly, in oral liquid.Effective ingredient ferulic acid and Ligustilide can influence the quality of the pharmaceutical preparations because decomposition causes content to reduce; In addition, adopt ethanol percolation to make percolate and the merging of all the other medicine concentrated solutions in the said preparation technology, with the dissolving of adjuvants such as sucrose, filter at last, add water move to total amount and form; Cause said preparation ethanol content height, to taking very big side effect puerperal especially women breast-feeding their children; And said preparation sucrose consumption is many, is unsuitable for diabetics and takes.We use the modern pharmaceutical technology this product technology are made significant improvement based on this, and make oral solid formulation, to solve the problem that this product exists, reach the purpose that improves curative effect of medication simultaneously.
Summary of the invention: the objective of the invention is to: Chinese medicine preparation of a kind of nourishing qi and blood and preparation method thereof is provided, this preparation is a solid preparation, comprise: capsule, tablet, granule, pill etc. are used for treatment of diseases such as gynaecopathia such as menoxenia, dysmenorrhea, blood deficiency be weak; Another object of the present invention is the preparation method of the Chinese medicine preparation of open these nourishing qi and bloods of preparation.In the present invention, we adopt the volatile oil in steam distillation extraction Radix Angelicae Sinensis and the Rhizoma Chuanxiong, avoided in the prior art direct percolation to cause the loss of volatile oil, this steam distillation extraction process also can overcome the lower shortcoming of conventional steam distillation extraction volatile oil yield in addition, and the volatile oil cyclodextrin inclusion compound of gained, thereby Radix Angelicae Sinensis, Rhizoma Chuanxiong volatile oil composition yield and effective ingredient Ligustilide thereof and content of ferulic acid and stability have been improved greatly.The present invention adopts spray-dired new technique in addition, utilize nebulizer that medical liquid spraying is become droplet, be scattered in the thermal current, moisture is evaporated rapidly, obtain the powdery loose particles, meet pyrolysated problem, effective ingredient is kept thereby overcome effective ingredient such as Ligustilide and ferulic acid.Simultaneously, the present invention by the optimal control to technical parameter, has effectively solved because the difficulty that the strong medical materials of viscosity such as Radix Rehmanniae, the Radix Paeoniae Alba are not easy to granulate in the prescription is dispersed in the granule it in spray-drying process.In addition, the granule of gained is with enclose material enclose, suppressed the hygroscopicity of Radix Rehmanniae, the Radix Paeoniae Alba, thereby controls the preparation moisture effectively, and the preparation of making has overcome the defective that existing product can not long term store.Compare with existing product, preparation of the present invention does not contain ethanol, has avoided the higher shortcoming of existing product ethanol content fully, the safety that has improved preparation greatly.The present invention can overcome the simple and poor stability of liquid preparation technology, contain the high drawback of alcohol amount, increase product stability by steam distillation extraction volatile oil, spray drying method and beta-cyclodextrin inclusion compound technology, improve the quality of products and curative effect, satisfy needs of medical treatment better.
The present invention constitutes like this: the Chinese medicine preparation of nourishing qi and blood: calculate according to composition by weight: it mainly is prepared from by Radix Angelicae Sinensis 200-1600, Radix Rehmanniae Preparata 12-96, Radix Paeoniae Alba 12-96, Rhizoma Chuanxiong 6-48, Radix Codonopsis 12-96, Radix Glycyrrhizae 6-48 and Radix Astragali 12-96 and adjuvant.Specifically: it mainly is prepared into oral solid formulation by Radix Angelicae Sinensis 800g, Radix Rehmanniae Preparata 48g, Radix Paeoniae Alba 48g, Rhizoma Chuanxiong 24g, Radix Codonopsis 48g, Radix Glycyrrhizae 24g and Radix Astragali 48g and adjuvant; Radix Rehmanniae Preparata steams with wine in the prescription, and the Radix Paeoniae Alba and Rhizoma Chuanxiong are fried with wine, Radix Glycyrrhizae and Radix Astragali processed with honey.Said oral solid formulation comprises capsule, tablet, granule or pill.The preparation method of the Chinese medicine preparation of nourishing qi and blood: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and uses cyclodextrin inclusion compound volatile oil, and is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, leaves standstill, incline and get supernatant, reclaim ethanol and be condensed into extractum, add the enclose material mixing and fully stir spray drying, add above-mentioned volatile oil clathrate compound and be mixed, add different adjuvants again and can make different solid preparations; The Radix Rehmanniae Preparata that uses steams with wine, and the Radix Paeoniae Alba and Rhizoma Chuanxiong are fried with wine, Radix Glycyrrhizae and Radix Astragali usefulness processed with honey.Concrete method: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the alpha-cyclodextrin that volatile oil is doubly measured with 0.5-3, beta-schardinger dextrin-or gamma-cyclodextrin enclose are standby; Medicinal residues and Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali mixes, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount and be 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, add the enclose material that recipe quantity 0.2-3 doubly measures: methylcellulose, the mixture of one or more in ethyl cellulose or the hydroxyethyl-cellulose, mix and fully stirring, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, add different adjuvants again and can make different solid preparations, said steam distillation extracting process is that medical material adds 5-10 times of water gaging, the ethyl acetate that adds 1.0-2.5% in the improvement oil water separator, distillation extraction 8-14h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, that is, the method for said cyclodextrin of volatile oil enclose is a saturated water solution method; The enclose temperature is 30-40 ℃, and ultrasonic time is 25-35min, and said spray-dired inlet temperature 155-180 ℃, leaving air temp 60-80 ℃, auxilliary pathogenic wind-warm degree is 50-120 ℃, temperature of charge≤70 ℃.
The preparation technology of described capsule is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water each 1-10 hour 1-5 time, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, contains the alcohol amount and is 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, add enclose material mixing and fully stirring that recipe quantity 0.2-3 doubly measures, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, incapsulate, promptly get capsule.
The preparation technology of described tablet is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount, left standstill 5-50 hour for 50-85%, incline and get supernatant, reclaim ethanol and be condensed into extractum, add enclose material mixing and fully stirring that recipe quantity 0.2-3 doubly measures, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, uses alcohol granulation, tabletting, with the 2-8% film coating agent plain sheet is carried out coating, promptly get tablet.
The preparation technology of described granule is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount for 50-85%, left standstill 5-50 hour, incline and get supernatant, reclaim ethanol and be condensed into thick paste, add dextrin and above-mentioned volatile oil clathrate compound mixing that 2-5 doubly measures, granulate, promptly get granule.
The preparation technology of described pill is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water each 1-10 hour 1-5 time, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, contains the alcohol amount and is 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, carry out spray drying, spray powder is pulverized, and adds above-mentioned volatile oil clathrate compound mixing, crosses the above screen cloth of 60 orders and 60 orders, the refined honey that adding medicated powder amount 0.5-2 doubly measures closes medicine, makes honeyed pill and gets final product.
In the present invention:
Said cyclodextrin the best is a beta-schardinger dextrin-.
Used steam distillation extracting process is, medical material adds 5-10 times of water gaging, adds the ethyl acetate of 1.0-2.5% in the improvement oil water separator, distillation extraction 8-14h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly.
The method of used cyclodextrin of volatile oil enclose is a saturated water solution method; The enclose temperature is 30-40 ℃, and ultrasonic time is 25-35min.
Used spray-dired inlet temperature 155-180 ℃, leaving air temp 60-80 ℃, auxilliary pathogenic wind-warm degree is 50-120 ℃, temperature of charge≤70 ℃.
The present invention is used for treatment of diseases such as gynaecopathia such as menoxenia, dysmenorrhea, blood deficiency be weak; Characteristics with nourishing qi and blood; The treatment menoxenia, puerperal, blood deficiency was weak, and anemia has curative effect preferably; Compared with prior art, the present invention adopts the volatile oil in steam distillation extraction Radix Angelicae Sinensis and the Rhizoma Chuanxiong, avoided in the prior art direct percolation to cause the loss of volatile oil, this steam distillation extraction process also overcomes the lower shortcoming of conventional steam distillation extraction volatile oil yield in addition, and the volatile oil cyclodextrin inclusion compound of gained, thereby Radix Angelicae Sinensis, Rhizoma Chuanxiong volatile oil composition yield and effective ingredient Ligustilide thereof and content of ferulic acid and stability have been improved greatly.The present invention adopts spray-dired new technique in addition, utilize nebulizer that medical liquid spraying is become droplet, be scattered in the thermal current, moisture is evaporated rapidly, obtain the powdery loose particles, meet pyrolysated problem, effective ingredient is kept thereby overcome effective ingredient such as Ligustilide and ferulic acid.Simultaneously, the present invention by the optimal control to technical parameter, has effectively solved because the difficulty that the strong medical materials of viscosity such as Radix Rehmanniae, the Radix Paeoniae Alba are not easy to granulate in the prescription is dispersed in the granule it in spray-drying process.In addition, the granule of gained is with enclose material enclose, suppressed the hygroscopicity of Radix Rehmanniae, the Radix Paeoniae Alba, thereby controls the preparation moisture effectively, and the preparation of making has overcome the defective that existing product can not long term store.Compare with existing product, preparation of the present invention does not contain ethanol, has avoided the higher shortcoming of existing product ethanol content fully, the safety that has improved preparation greatly.The present invention can overcome the simple and poor stability of liquid preparation technology, contain the high drawback of alcohol amount, increase product stability by steam distillation extraction volatile oil, spray drying method and beta cyclodextrin inclusion technique, improve the quality of products and curative effect, satisfy needs of medical treatment better; Reached the purpose of invention.
Pharmacological effect:
The present invention tests 1:
Stability experiment
(1) experiment material:
1, medicine: blood-bonifying Chinese angelica capsules A: according to method preparation of the present invention.Be assigned to desired concn with normal saline during experiment.The smart B of blood-bonifying Chinese angelica: commercially available.The blood-bonifying Chinese angelica decoction C that the prepared slices of Chinese crude drugs are made.
2, animal: Japan large ear rabbit.
(2), method and result:
1, test method: above sample is positioned in 3-5 ℃, 20-25 ℃, 33-37 ℃ the calorstat, makes regular check on stability indicator according to time of repose, the criterion that its result observes is as follows:
0 does not have precipitation 1 does not have muddy 2 little turbid 3 muddy 4 precipitations
2, result of the test: result of the test sees the following form.
| Project | 3h | 5h | 8h | 14h | 21h | ||||||||||
| A | B | C | A | B | C | A | B | C | A | B | C | A | B | C | |
| 3℃-5℃ | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 1 | 3 | 0 | 2 | 4 | 0 | 3 | 4 |
| 20℃-25℃ | 0 | 0 | 2 | 0 | 2 | 4 | 0 | 2 | 3 | 0 | 3 | 4 | 0 | 4 | 4 |
| 33℃-37℃ | 0 | 0 | 2 | 0 | 2 | 4 | 0 | 2 | 4 | 0 | 2 | 3 | 0 | 4 | 4 |
3, conclusion (of pressure testing): by this result of the test as can be known, the blood-bonifying Chinese angelica capsules A does not have muddiness, precipitation produces; The smart B of blood-bonifying Chinese angelica has a small amount of muddy generation; The blood-bonifying Chinese angelica decoction C that the prepared slices of Chinese crude drugs are made has muddiness, precipitation to produce.
(3), brief summary: show by study on the stability test: under the same conditions, the blood-bonifying Chinese angelica decoction C that the blood-bonifying Chinese angelica capsules A is made than the smart B of blood-bonifying Chinese angelica and the prepared slices of Chinese crude drugs stablizes.
The present invention tests 2:
Will be under the preparation normal condition of the present invention place 30 months, and survey its water content, result such as following table during respectively at 3,6,9,12,18,24,30 months.According to testing result, preparation of the present invention its water content in 30 months changes little.
The present invention tests 3:
Get blood-bonifying Chinese angelica smart 125ml, blood-bonifying Chinese angelica capsule 's content 15g, the blood-bonifying Chinese angelica ball is got 50g, blood-bonifying Chinese angelica sheet 15g, blood-bonifying Chinese angelica granule 40g after smashing to pieces, all is equivalent to the about 50g of protogenic medicinal powder.Detect the wherein content of ferulic acid and Ligustilide respectively, the result is:
| Project | Ferulic acid | Ligustilide |
| The blood-bonifying Chinese angelica essence | 7.0253mg | 29.215mg |
| The blood-bonifying Chinese angelica capsule | 13.695mg | 156.025mg |
| The blood-bonifying Chinese angelica soft gelatin capsule | 11.720mg | 149.235mg |
| The blood-bonifying Chinese angelica sheet | 12.861mg | 134.531mg |
| The blood-bonifying Chinese angelica granule | 13.128mg | 129.429mg |
Show according to above experimental result: preparation Ligustilide of the present invention and content of ferulic acid are apparently higher than the blood-bonifying Chinese angelica essence.
The present invention tests 4:
Preparation of the present invention is to normal effect of immunologic function
One, experiment material
1, medicine: the blood-bonifying Chinese angelica sheet, according to method preparation of the present invention; The blood-bonifying Chinese angelica essence, commercially available; Normal saline.Lentinan film, Guangshui, Hubei Province pharmaceutical factory produces; The plant blood clotting.
2, animal Kunming mouse, Cavia porcellus is planted by Britain.
Two, method and result
1, to the influence of Turnover of Mouse Peritoneal Macrophages phagocytic function: get 40 of mices, body weight 18～20g, male and female half and half, evenly be divided into 4 groups immediately, gavage the smart solution of blood-bonifying Chinese angelica sheet solution, blood-bonifying Chinese angelica, lentinan film solution respectively and with the volume normal saline, administration every day 1 time, successive administration 7 days.1h after last 1 administration, every Mus lumbar injection 5% chicken red blood cell suspension 0.4ml/ only in to 6h behind the chicken red blood cell, put to death mice, survey the huge function of biting of peritoneal macrophage by literature method, the results are shown in following table.
Preparation of the present invention is to the X ± S that influences of normal Turnover of Mouse Peritoneal Macrophages phagocytic function
| Group | Number of animals n | Dosage mgkg -1 | Phagocytic percentage % | Phagocytic index |
| The normal saline group | 10 | - | 46.65±3.7 | 0.65±0.10 |
| The lentinan group | 10 | 50 | 55.5±3.9 ** | 0.75±0.83 ** |
| Blood-bonifying Chinese angelica sheet group | 10 | 100 | 61.9±3.6 ** | 0.92±0.79 ** |
| The smart group of blood-bonifying Chinese angelica | 10 | 100 | 57.4±6.0 ** | 0.72±0.83 * |
With normal saline group ratio
*P＜0.05,
*P＜0.01
2, the influence that the normal mouse hemolysin is formed: mice number of animals, grouping, administration are all the same, in administration the 1st day, every Mus lumbar injection 5% chicken red blood cell suspension 0.2ml/ only, carry out immunity, 2h after last 1 administration, mouse orbit is got blood, presses literature method and surveys hemolysin formation, the results are shown in following table.
3, the influence that the normal mouse hemolysis plaque is formed: mice number of animals, grouping, administration and immunity are all the same, 2h after last 1 administration, mice is taken off neck puts to death, dissect and take out spleen, two mouse spleens are put together use homogenizer homogenate, press literature method and survey hemolysis plaque formation, the results are shown in following table.
4, the influence that normal mouse lymphocyte is transformed: mice number of animals, grouping, administration are all the same, in preceding 3 days of administration, the equal intramuscular injection PHA8mg/kg of every Mus every day, 2h after last 1 administration, mice is cut tail and gets blood, smear, Switzerland's dye liquor dyeing, the conversion ratio of oily sem observation peripheral blood lymphocyte the results are shown in following table.
Preparation of the present invention forms and the LT X ± S that influences normal mouse hemolysin, hemolysis plaque
| Group | Number of animals only | Dosage g/kg | Hemolysin forms A | Lymphocyte transformation % | The example several | Hemolysis plaque forms A |
| The normal saline group | 10 | - | 0.133±0.072 | 42.0±2.4 | 5 | 0.377±0.055 |
| The lentinan group | 10 | 50 | 0.181±0.071 | 45.7±4.6 * | 5 | 0.469±0.039 ** |
| Blood-bonifying Chinese angelica sheet group | 10 | 100 | 0.212±0.040 ** | 45.1±3.3 ** | 5 | 0.472±0.054 * |
| The smart group of blood-bonifying Chinese angelica | 10 | 100 | 0.191±0.057 * | 42.8±5.7 | 5 | 0.394±0.069 * |
With normal saline group ratio
*P＜0.05,
*P＜0.01
Three, conclusion:
From as can be seen last, the smart group of blood-bonifying Chinese angelica sheet group and blood-bonifying Chinese angelica relatively, more can significantly improve the normal mouse peritoneal macrophage huge bite percentage rate and phagocytic index, obviously promote formation of normal mouse hemolysin and lymhocyte transformation rate thereof, obviously promote the formation of hemolysis plaque, so the immunologic function of blood-bonifying Chinese angelica sheet is smart strong than blood-bonifying Chinese angelica.
The present invention tests 5:
Preparation of the present invention causes the influence of mice model of blood dificiency to cyclophosphamide and acetylphenylhydrazine coupling
One, experiment material
Medicine: the blood-bonifying Chinese angelica capsule, according to method preparation of the present invention; The blood-bonifying Chinese angelica essence, commercially available; Normal saline.Acetylphenylhydrazine.
Two, method and result: get 40 of mices, body weight 18g～20g, male and female half and half.Be divided into 4 groups at random, wherein 3 groups to gavage blood-bonifying Chinese angelica capsule, blood-bonifying Chinese angelica respectively smart and with the volume normal saline, successive administration 7d.Normal saline group and administration group mice are respectively at the 2nd, the 4th subcutaneous injection acetylphenylhydrazine 20mg/kg, 40mg/kg of administration, every Mus intraperitoneal injection of cyclophosphamide 40mg/kg from the 4th day, continuous 4d, other establishes a complete blank group, gavage and inject normal saline with volume, 2h after the last administration, mice is cut the tail blood sampling, the reference literature method is observed mice RBC, WBC, Hb value, the results are shown in following table.
Preparation of the present invention is to the x ± s that influences of mice model of blood dificiency RBC, WBC, Hb level
| Group | Number of animals n | Dosage mgkg -1 | RBC ×10 12Individual L -1 | WBC ×10 9Individual L -1 | Hb gL -1 |
| The blank group | 10 | - | 7.458±0.627 | 9.01±0.95 | 105±1 |
| The normal saline group | 10 | - | 3.075 ±0.297 △△ | 3.14±0.52 △△ | 67±7 △△ |
| The blood-bonifying Chinese angelica Capsules group | 10 | 100 | 4.162±0.627 △△ | 4.27±1.21 △△ | 80±7 △△ |
| The smart group of blood-bonifying Chinese angelica | 10 | 100 | 3.727±0.685 △ | 3.96±1.38 △ | 74±6 △△ |
Annotate: compare with model group: △ P＜0.05, △ △ P＜0.01
Three, discuss: can find out that from last table compare with the blank group, normal saline group mice hemogram significantly reduces P＜0.01, and the success of hemopoietic virtual model is described; Compare with the blank group, blood-bonifying Chinese angelica capsule and blood-bonifying Chinese angelica essence all can significantly raise blood deficiency mice RBC, WBC and Hb level, but the elevated levels of blood-bonifying Chinese angelica Capsules group blood deficiency mice RBC, WBC and Hb is smart than blood-bonifying Chinese angelica to be high, so the capsular blood tonification effect of blood-bonifying Chinese angelica is good than the blood-bonifying Chinese angelica essence.
The specific embodiment:
The embodiment of the invention 1:
Take by weighing: Radix Angelicae Sinensis 800g, Radix Rehmanniae Preparata (wine steams) 48g, the Radix Paeoniae Alba (wine stir-fry) 48g, Rhizoma Chuanxiong (wine stir-fry) 24g, Radix Codonopsis 48g, Radix Glycyrrhizae (processed with honey) 24g, the Radix Astragali (processed with honey) 48g, beta-schardinger dextrin-20g, methylcellulose 1620g, ethyl cellulose 1500g
Preparation technology is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, method is: get Radix Angelicae Sinensis, Rhizoma Chuanxiong adding 6592ml water, add 2% ethyl acetate in the improvement oil water separator, distillation extraction l0h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, promptly.Volatile oil beta-cyclodextrin inclusion compound, enclose temperature are 30-40 ℃, and ultrasonic time is 30-35min, and is standby.Medicinal residues and Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali mixes, decoct with water 2 times, each 4 hours, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, containing the alcohol amount is 70%, leaves standstill 24 hours, inclines and gets supernatant, reclaim ethanol and be condensed into extractum, add methylcellulose, ethyl cellulose mixes and fully stirs, and crosses 60 mesh filter screens, feeds hot-air under atomize, inlet temperature 160-170 ℃, leaving air temp 65-75 ℃, 75-85 ℃ of auxilliary pathogenic wind-warm degree, temperature of charge 40-60 ℃, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, incapsulates, and promptly gets capsule.
The embodiment of the invention 2:
Take by weighing: Radix Angelicae Sinensis 1600g, Radix Rehmanniae Preparata 96g, Radix Paeoniae Alba 96g, Rhizoma Chuanxiong 48g, Radix Codonopsis 96g, Radix Glycyrrhizae 48g, Radix Astragali 96g, alpha-cyclodextrin 120g, hydroxyethyl-cellulose 416g
Preparation technology is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, method is: get Radix Angelicae Sinensis, Rhizoma Chuanxiong adding 16480ml water, add 2.5% ethyl acetate in the improvement oil water separator, distillation extraction 8h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, promptly.Volatile oil alpha-cyclodextrin enclose, enclose temperature are 30-40 ℃, and ultrasonic time is 25-30min, and is standby; Medicinal residues and Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali mixes, and decocts with water each 1 hour 5 times, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, and containing the alcohol amount is 85%, left standstill 50 hours, incline and get supernatant, reclaim ethanol and be condensed into extractum, add hydroxyethyl-cellulose and mix also abundant the stirring, cross 100 mesh filter screens, under atomize, feed hot-air, inlet temperature 170-180 ℃, leaving air temp 70-80 ℃, 100-120 ℃ of auxilliary pathogenic wind-warm degree, temperature of charge 60-70 ℃, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, use alcohol granulation, tabletting carries out coating with the 2-8% film coating agent to plain sheet, promptly gets tablet.
The embodiment of the invention 3:
Take by weighing: Radix Angelicae Sinensis 200g, Radix Rehmanniae Preparata (wine steams) 12g, the Radix Paeoniae Alba (wine stir-fry) 12g, Rhizoma Chuanxiong (wine stir-fry) 6g, Radix Codonopsis 12g, Radix Glycyrrhizae (processed with honey) 6g, the Radix Astragali (processed with honey) 12g, gamma-cyclodextrin 15g, refined honey 100g
Preparation technology is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, method is: get Radix Angelicae Sinensis, Rhizoma Chuanxiong adding 1030ml water, add 1.0% ethyl acetate in the improvement oil water separator, distillation extraction 14h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, promptly.Volatile oil gamma-cyclodextrin enclose, enclose temperature are 30-40 ℃, and ultrasonic time is 25-30min.Standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 10 hours, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, and containing the alcohol amount is 50%, left standstill 5 hours, incline and get supernatant, reclaim ethanol and be condensed into extractum, under atomize, feed hot-air, inlet temperature 155-160 ℃, leaving air temp 60-70 ℃, 50-70 ℃ of auxilliary pathogenic wind-warm degree, temperature of charge≤40-50 ℃, carry out spray drying, spray powder is pulverized, and adds above-mentioned volatile oil clathrate compound mixing, crosses 100 purpose screen clothes, add medicated powder amount refined honey and close medicine, make honeyed pill and get final product.
The embodiment of the invention 4:
Take by weighing: Radix Angelicae Sinensis 800g, Radix Rehmanniae Preparata 48g, Radix Paeoniae Alba 48g, Rhizoma Chuanxiong 24g, Radix Codonopsis 48g, Radix Glycyrrhizae 24g, Radix Astragali 48g, beta-schardinger dextrin-10g, dextrin 1600g
Preparation technology is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, method is: get Radix Angelicae Sinensis, Rhizoma Chuanxiong adding 6592ml water, add 1.5% ethyl acetate in the improvement oil water separator, distillation extraction 12h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, promptly.Volatile oil beta-cyclodextrin inclusion compound, enclose temperature are 30-40 ℃, and ultrasonic time is 30-35min.Standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 3 times, each 3 hours, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, containing the alcohol amount is 65%, leaves standstill 24 hours, inclines and gets supernatant, reclaim ethanol and be condensed into thick paste, add dextrin and above-mentioned volatile oil clathrate compound mixing, granulate, promptly get granule.
Claims (9)
1, a kind of Chinese medicine preparation of nourishing qi and blood is characterized in that: calculate according to composition by weight: it mainly is prepared from by Radix Angelicae Sinensis 200-1600, Radix Rehmanniae Preparata 12-96, Radix Paeoniae Alba 12-96, Rhizoma Chuanxiong 6-48, Radix Codonopsis 12-96, Radix Glycyrrhizae 6-48 and Radix Astragali 12-96 and adjuvant.
2, according to the Chinese medicine preparation of the described nourishing qi and blood of claim 1, it is characterized in that: it mainly is prepared into oral solid formulation by Radix Angelicae Sinensis 800g, Radix Rehmanniae Preparata 48g, Radix Paeoniae Alba 48g, Rhizoma Chuanxiong 24g, Radix Codonopsis 48g, Radix Glycyrrhizae 24g and Radix Astragali 48g and adjuvant; Radix Rehmanniae Preparata steams with wine in the prescription, and the Radix Paeoniae Alba and Rhizoma Chuanxiong are fried with wine, Radix Glycyrrhizae and Radix Astragali processed with honey.
3, according to the Chinese medicine preparation of claim 1 or 2 described nourishing qi and bloods, it is characterized in that: said oral solid formulation comprises capsule, tablet, granule or pill.
4, as the preparation method of the Chinese medicine preparation of any described nourishing qi and blood among the claim 1-3, it is characterized in that: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and uses cyclodextrin inclusion compound volatile oil, and is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, leaves standstill, incline and get supernatant, reclaim ethanol and be condensed into extractum, add the enclose material mixing and fully stir spray drying, add above-mentioned volatile oil clathrate compound and be mixed, add different adjuvants again and can make different solid preparations; The Radix Rehmanniae Preparata that uses steams with wine, and the Radix Paeoniae Alba and Rhizoma Chuanxiong are fried with wine, Radix Glycyrrhizae and Radix Astragali usefulness processed with honey.
5, according to the preparation method of the Chinese medicine preparation of the described nourishing qi and blood of claim 4, it is characterized in that: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the alpha-cyclodextrin that volatile oil is doubly measured with 0.5-3, beta-schardinger dextrin-or gamma-cyclodextrin enclose are standby; Medicinal residues and Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali mixes, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount and be 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, add the enclose material that recipe quantity 0.2-3 doubly measures: methylcellulose, the mixture of one or more in ethyl cellulose or the hydroxyethyl-cellulose, mix and fully stirring, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, add different adjuvants again and can make different solid preparations, said steam distillation extracting process is that medical material adds 5-10 times of water gaging, the ethyl acetate that adds 1.0-2.5% in the improvement oil water separator, distillation extraction 8-14h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, that is, the method for said cyclodextrin of volatile oil enclose is a saturated water solution method; The enclose temperature is 30-40 ℃, and ultrasonic time is 25-35min, and said spray-dired inlet temperature 155-180 ℃, leaving air temp 60-80 ℃, auxilliary pathogenic wind-warm degree is 50-120 ℃, temperature of charge≤70 ℃.
6, according to the preparation method of the Chinese medicine preparation of claim 4 or 5 described nourishing qi and bloods, it is characterized in that: the preparation technology of described capsule is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water each 1-10 hour 1-5 time, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, contains the alcohol amount and is 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, add enclose material mixing and fully stirring that recipe quantity 0.2-3 doubly measures, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, incapsulate, promptly get capsule.
7, according to the preparation method of the Chinese medicine preparation of claim 4 or 5 described nourishing qi and bloods, it is characterized in that: the preparation technology of described tablet is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount, left standstill 5-50 hour for 50-85%, incline and get supernatant, reclaim ethanol and be condensed into extractum, add enclose material mixing and fully stirring that recipe quantity 0.2-3 doubly measures, cross the 30-100 mesh filter screen, carry out spray drying, spray powder adds above-mentioned volatile oil clathrate compound mixing, uses alcohol granulation, tabletting, with the 2-8% film coating agent plain sheet is carried out coating, promptly get tablet.
8, according to the preparation method of the Chinese medicine preparation of claim 4 or 5 described nourishing qi and bloods, it is characterized in that: the preparation technology of described granule is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water 1-5 time, each 1-10 hour, merging filtrate, concentrate the back and add the ethanol precipitate with ethanol, contain the alcohol amount for 50-85%, left standstill 5-50 hour, incline and get supernatant, reclaim ethanol and be condensed into thick paste, add dextrin and above-mentioned volatile oil clathrate compound mixing that 2-5 doubly measures, granulate, promptly get granule.
9, according to the preparation method of the Chinese medicine preparation of claim 4 or 5 described nourishing qi and bloods, it is characterized in that: the preparation technology of described pill is: get Radix Angelicae Sinensis, the chopping of Rhizoma Chuanxiong medical material, water steam distillation and extraction method is extracted volatile oil, and the cyclodextrin inclusion compound that volatile oil is doubly measured with 0.5-3 is standby; Medicinal residues mix with Radix Rehmanniae Preparata, the Radix Paeoniae Alba, Radix Codonopsis, Radix Glycyrrhizae, the Radix Astragali, decoct with water each 1-10 hour 1-5 time, merging filtrate concentrates the back and adds the ethanol precipitate with ethanol, contains the alcohol amount and is 50-85%, left standstill 5-50 hour, and inclined and get supernatant, reclaim ethanol and be condensed into extractum, carry out spray drying, spray powder is pulverized, and adds above-mentioned volatile oil clathrate compound mixing, crosses the above screen cloth of 60 orders and 60 orders, the refined honey that adding medicated powder amount 0.5-2 doubly measures closes medicine, makes honeyed pill and gets final product.
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