CN1836507A - Medicine for preventing and treating gadol withered disease - Google Patents
Medicine for preventing and treating gadol withered disease Download PDFInfo
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- CN1836507A CN1836507A CN 200610016806 CN200610016806A CN1836507A CN 1836507 A CN1836507 A CN 1836507A CN 200610016806 CN200610016806 CN 200610016806 CN 200610016806 A CN200610016806 A CN 200610016806A CN 1836507 A CN1836507 A CN 1836507A
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Abstract
The germicide preparation for preventing and controlling the damping-off of rhodiola is compounded with juglone, including 5-hydroxy-1,4-naphthoquinone and 5,8-dihydroxy-1,4-naphthoquinone, as the effective component in green fruit peel and leaf of Juglans mandshurica. It exhibits excellent effect of inhibiting the growth of ptotomiliary body of damping-off pathogen and is effective preparation for preventing and controlling the damping-off of rhodiola.
Description
Technical field
The present invention relates to the exploitation of the anti-disease medicines of traditional Chinese medicine, particularly a kind of medicine of preventing and treating the rhodiola root seedling blight.
Background technology
The cultivation of traditional Chinese medicine is the important economic development approach in current China mountain area.The present situation of current China traditional Chinese medicine cultivation shows that the various damage by disease and insect of control traditional Chinese medicine are to ensure the just important step of high-quality high yield of Chinese medicine.But be to use synthetic pesticide must cause the residual of farm chemical ingredients, influence the medicinal effects of traditional Chinese medicine, can't export simultaneously.Therefore, be badly in need of the close environment agricultural chemicals of exploitation noresidue.For this reason, domestic and international many researchers make great efforts to develop biopesticide at present.Botanical pesticide belongs to the biopesticide category, is the class medicine with pesticide activity that obtains through physico-chemical process from plant.Because endophytic active component forms in the plant metabolism process, can degrade in natural environment, therefore seldom bring residual.On the other hand, can provide the guide thing for novel pesticide developing.
Sachalin rhodiola rhizome (Rhodiola sachalinensis A.Bor.) is a Crassulaceae Sedum herbaceos perennial, mainly is distributed in Changbaishan area and Daxing'an Mountainrange one band.Result of study in recent years shows that sachalin rhodiola rhizome has anti-hypoxia, antifatigue, resisting microwave radiation, delays body aging and prevents and treats functions such as infirmities of age, is a kind of famous and precious traditional Chinese medicine.But because the sachalin rhodiola rhizome distribution is narrow, its habitat is abominable, and natural renewal power is low, adds in recent years and excavates in a large number, causes its resource to be seriously damaged, and has been in Critical Condition.
Therefore the artificial cultivation of rhodiola root is imperative.In rhodiola root artificial cultivation process, find the normal damping off that takes place on 1 year living seedbed, have a strong impact on rhodiola root and grow seedlings, and then influence the cultivation of rhodiola root.
The people such as Shi Tieyuan of agricultural college of Yanbian University distinguish that through years of researches the gadol withered disease cause of disease is Rhizoctonia solani Kuhn (Rhizoctonia solani Kuhn).Human carbendazim (Carbendazinm) such as Shi Tieyuan, dimethachlon synthetic pesticides such as (Dumethachlon) have carried out the efficiency test of gadol withered disease, have obtained gratifying result.
As everyone knows, the medium-term and long-term and a large amount of synthetic pesticides that use of the production of cereal crops are key factors that cause agroecological destruction, hinder the agricultural economy sustainable development, are the hidden danger that threatens human health.In the cultivation for the traditional Chinese medicine of promoting human health longevity, using synthetic pesticide is mysterious behavior.
Summary of the invention
The objective of the invention is to provide a kind of close environment medicine of noresidue, be the damping off of preventing and treating rhodiola root effectively botanical pesticide is provided for the cultivation of giving rhodiola root.
In the research process for the botanical pesticide of starting close environment, the inventor attempts to seek the plant component of a desirable control rhodiola root seedling blight, through repeatedly and many-sided test has obtained satisfied PRELIMINARY RESULTS.
Studies have shown that, juglone (5-hydroxyl-1,4-naphthoquinones) and derivative 5 thereof, 8-dihydroxy-1, the 4-naphthoquinones is the important active substance in Semen Juglandis peel, the leaf, shows various physiologically actives.In our research, find this two materials, can suppress the growth of gadol withered disease cause of disease Rhizoctonia solani Kuhn effectively.For this reason, technical scheme provided by the invention is:
A kind of medicine of preventing and treating gadol withered disease wherein contains juglone.
A kind of medicine of preventing and treating gadol withered disease wherein contains 5,8-dihydroxy-1,4-naphthoquinones.
A kind of medicine of preventing and treating gadol withered disease wherein contains 30.0mg/L～36.0mg/L juglone; The juglone that preferentially contains 34.4mg/L concentration.
A kind of medicine of preventing and treating gadol withered disease wherein contains 5 of 25.0mg/L～30.0mg/L concentration, 8-dihydroxy-1,4-naphthoquinones; Preferentially contain 5 of 26.4mg/L concentration, 8-dihydroxy-1,4-naphthoquinones.
Come in conjunction with explanation concrete actual effect of the present invention below by specific embodiment:
Embodiment
Since juglone and 5 in Semen Juglandis peel and the leaf crude extract, 8-dihydroxy-1, and the content of 4-naphthoquinones is very low, be difficult to obtain enough amounts, therefore, (SyntheticCommunications 15 (13) according to Antonio V.Pinto, 1177-80,1985) method, from 1, the 5-dihydroxy naphthlene, yield with 30% obtains orange-yellow juglone, mp, 152-4 ℃
1H-NMR (300Hz, CDCl
3) δ (ppm): 11,9 (1H, s), 7,56-7,68 (2H, m), 7,19-7,39 (1H, m), 6,96 (2H, s); Again according to Zahn, V.K (Justus Liebigs Annalen der chemie, 462,72-97, nineteen twenty-eight) people's method such as, from the reaction of hydroquinones and maleic anhydride, the yield with 15% obtains aubergine crystallization 5,8-dihydroxy-1, the 4-naphthoquinones, mp, 235-7 ℃
1H-NMR (300Hz, CDCl
3) δ (ppm): 12,4 (2H, s), 7,15 (4H, s).
Former medicine of the present invention can be prepared as follows:
The preparation of juglone medicament: get the 1.07g juglone, be dissolved in 50ml ethyl acetate and 50ml ethanol, add the 2.17g TritonX, obtain soup 103ml, concentration is 10.32g/L;
5,8-dihydroxy-1, the preparation of 4-naphthoquinones medicament: get 1.07g 5,8-dihydroxy-1, the 4-naphthoquinones is dissolved in 50ml ethanol and 83ml oxolane, adds the 2.17g TritonX, obtains soup 135ml, and concentration is 7.92g/L.
Test method: growth rate determination method
Plant pathogenic fungi is 25 ℃ of constant temperature culture 7d in PSA medium plate, treat to be for experiment after bacterium is covered with in plate.In addition, the medium that is for experiment, water and pipettor etc. are with wet-hot steam sterilization 30 minutes, and culture dish, card punch and tweezers etc. are placed in the drying box 165 ℃ of hot air sterilizations 1 hour after wrapping with newspaper.After sterilization finishes, move into immediately to be equipped with in the superclean bench of sterilizing 30 minutes to test and use.Accurately draw the 0.5ml medicine liquid droplet in sterilized triangular flask with pipettor when treating that medium is cooled to 50 ℃ of left and right sides, then medium is poured into fully to shake up and made 100 times of liquid to 50ml, in like manner, soup is diluted again successively for falling dull and stereotyped behind the 200-800 times of liquid, adding medicine is not a blank, and every processing repeats 3 times.
Treat that the pastille medium solidifies in plate after, with aseptic card punch the little bacterium cake of diameter 6mm is bought at the open and flat place of the mycelia of plant pathogenic fungi, above being attempted by, every ware connects 1 piece of bacterium cake.Under 25 ℃ of conditions, measure the growth diameter effect of the inhibition bacterium colony of different medicaments after cultivation a period of time with the right-angled intersection mensuration.
Colony growth diameter=3 time diameter mean value-6.0mm.
Mycelial growth inhibition rate=(contrast colony growth diameter-processing colony growth diameter)/contrast colony growth diameter * 100%
Example 1, the former medicine of the above-mentioned juglone of dilute with water is mixed with various concentration, has checked the inhibition effect for the growth of gadol withered disease cause of disease Rhizoctonia solani Kuhn.Its result such as table 1.
Table 1
| Medicament | Concentration (mg/L) | Colony diameter (mm) | Inhibiting rate |
| Juglone | 103.2 | 6 | 100 |
| 51.6 | 17.33 | 86.51 | |
| 34.4 | 19.67 | 83.73 | |
| 25.8 | 25.17 | 77.18 | |
| 20.64 | 28.33 | 73.41 | |
| 17.2 | 33.67 | 67.06 | |
| 14.74 | 40.33 | 59.13 | |
| 12.9 | 44.50 | 54.17 | |
| CK | - | 90 | - |
Example 2, in like manner, dilute with water is above-mentioned 5,8-dihydroxy-1, the former medicine of 4-naphthoquinones is mixed with various concentration, has checked the inhibition effect for the growth of gadol withered disease cause of disease Rhizoctonia solani Kuhn.Its result such as table 2.
Table 2,
| Medicament | Concentration (mg/L) | Colony diameter (mm) | Inhibiting rate |
| 5,8-dihydroxy-1,4-naphthoquinones | 79.2 | 6 | 100 |
| 39.6 | 6 | 100 | |
| 26.4 | 21.33 | 81.75 | |
| 19.8 | 27.67 | 74.21 | |
| 15.84 | 32.83 | 68.06 | |
| 13.2 | 36.33 | 63.89 | |
| 11.31 | 43.33 | 55.56 | |
| 9.9 | 49.33 | 48.41 | |
| CK | - | 90 | - |
From above assay, important component juglone and 5 in Semen Juglandis peel and the bark, 8-dihydroxy-1, the 4-naphthoquinones, respectively when 34.4mg/L and 26.4mg/L concentration, showing the effect of the growth of good inhibition gadol withered disease cause of disease Rhizoctonia solani Kuhn, be the active drug of control rhodiola root seedling blight, and cost is lower.
Claims (6)
1, a kind of medicine of preventing and treating gadol withered disease wherein contains juglone.
2, a kind of medicine of preventing and treating gadol withered disease wherein contains 5,8-dihydroxy-1,4-naphthoquinones.
3, a kind of medicine of preventing and treating gadol withered disease according to claim 1 wherein contains 30.0mg/L～36.0mg/L juglone.
4, a kind of medicine of preventing and treating gadol withered disease according to claim 3 wherein contains the 34.4mg/L juglone.
5, a kind of medicine of preventing and treating gadol withered disease according to claim 2 wherein contains 5 of 25.0mg/L～30.0mg/L concentration, 8-dihydroxy-1,4-naphthoquinones.
6, a kind of medicine of preventing and treating gadol withered disease according to claim 5 wherein contains 5 of 26.4mg/L concentration, 8-dihydroxy-1,4-naphthoquinones.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016806 CN1836507A (en) | 2006-04-22 | 2006-04-22 | Medicine for preventing and treating gadol withered disease |
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| CN 200610016806 CN1836507A (en) | 2006-04-22 | 2006-04-22 | Medicine for preventing and treating gadol withered disease |
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| CN 200610016806 Pending CN1836507A (en) | 2006-04-22 | 2006-04-22 | Medicine for preventing and treating gadol withered disease |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028677A (en) * | 2010-11-24 | 2011-04-27 | 广东省农业科学院兽医研究所 | Application of juglone nucin to resistance to eimeria tenella |
| CN102626113A (en) * | 2012-03-26 | 2012-08-08 | 王福 | Method for extracting pesticide from juglans mandshurica fruit green peels and leaves |
| CN104855378A (en) * | 2015-05-20 | 2015-08-26 | 沈阳农业大学 | Medicament for inhibiting root growth of stored garlic |
| WO2011103610A3 (en) * | 2010-02-24 | 2015-09-03 | Technische Universität Wien | Plant protection agent |
| JP2023507069A (en) * | 2019-11-15 | 2023-02-21 | ベクスタケム ソシエタ ア レスポンサビリタ リミタータ | Phytosanitary agents and specific uses thereof in the treatment of plants infected with Pierce's fungus |
-
2006
- 2006-04-22 CN CN 200610016806 patent/CN1836507A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011103610A3 (en) * | 2010-02-24 | 2015-09-03 | Technische Universität Wien | Plant protection agent |
| CN102028677A (en) * | 2010-11-24 | 2011-04-27 | 广东省农业科学院兽医研究所 | Application of juglone nucin to resistance to eimeria tenella |
| CN102028677B (en) * | 2010-11-24 | 2012-05-30 | 广东省农业科学院兽医研究所 | Application of juglone in preparation of anti-eimeria tenella medicines |
| CN102626113A (en) * | 2012-03-26 | 2012-08-08 | 王福 | Method for extracting pesticide from juglans mandshurica fruit green peels and leaves |
| CN104855378A (en) * | 2015-05-20 | 2015-08-26 | 沈阳农业大学 | Medicament for inhibiting root growth of stored garlic |
| JP2023507069A (en) * | 2019-11-15 | 2023-02-21 | ベクスタケム ソシエタ ア レスポンサビリタ リミタータ | Phytosanitary agents and specific uses thereof in the treatment of plants infected with Pierce's fungus |
| JP7462042B2 (en) | 2019-11-15 | 2024-04-04 | ベクスタケム ソシエタ ア レスポンサビリタ リミタータ | Phytosanitary agents and their specific use in the treatment of plants infected with Pierce's disease fungus - Patents.com |
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