CN104855378A - Medicament for inhibiting root growth of stored garlic - Google Patents

Medicament for inhibiting root growth of stored garlic Download PDF

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Publication number
CN104855378A
CN104855378A CN201510275277.XA CN201510275277A CN104855378A CN 104855378 A CN104855378 A CN 104855378A CN 201510275277 A CN201510275277 A CN 201510275277A CN 104855378 A CN104855378 A CN 104855378A
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CN
China
Prior art keywords
medicament
garlic
juglone
root
cosolvent
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Pending
Application number
CN201510275277.XA
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Chinese (zh)
Inventor
檀德宏
纪淑娟
白冰
吴尚仪
刘明鸣
刘琦
张旭旭
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Shenyang Agricultural University
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Shenyang Agricultural University
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Priority to CN201510275277.XA priority Critical patent/CN104855378A/en
Publication of CN104855378A publication Critical patent/CN104855378A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a medicament for inhibiting root growth of stored garlic. The medicament is a water solution containing juglone, wherein the concentration of juglone is 0.001-1%; juglone is wide in raw material source, can be used as an extract of a natural juglone plant and also juglone synthesized chemically, and is easy to prepare and low in cost. The medicament is convenient to use; the garlic is only soaked with the medicament for a while and then aired; the medicament is low in juglone dosage, non-toxic, environment-friendly, and capable of effectively inhibiting root growth of the garlic and effectively preventing garlic rot, and cannot inhibit seedling growth of the garlic.

Description

A kind of medicament suppressing storage garlic to take root
Technical field
The present invention relates to a kind of rooting inhibition of cuttings agent, particularly relate to a kind of medicament suppressing storage garlic to take root.
Background technology
Taking root is the problem needing in garlic storage to solve.Garlic, after resting stage, there will be problems such as taking root, makes garlic lose commodity value.Garlic is suppressed to take root extremely important to garlic storage.
Suppress the method that garlic takes root to have low temperature storage, Radiation, scald the methods such as garlic root with hot water traditionally, but these methods are subject to a definite limitation, garlic is difficult to accomplish low temperature storage in logistics, sales section; Radiation needs special installation, not easily spread; Hot water scalds garlic root and garlic can be caused to rot, and practicality is not strong.Thus be necessary that the new suppression garlic of research and development takes root material or method.
Summary of the invention
The object of this invention is to provide a kind of medicament suppressing storage garlic to take root, it conveniently can solve the problem that storage garlic takes root.
The present invention is achieved through the following technical solutions:
Suppress the medicament that storage garlic takes root, it is characterized in that, this medicament is the aqueous solution containing juglone and cosolvent, and the concentration of described juglone is 0.001-1%.
The concentration of described juglone is preferably 0.01-1%.
Described cosolvent is tween or alcohol.Described cosolvent is preferably Tween 80.
The concentration of juglone of the present invention is all mass body volume concentrations.
Suppress the medicament that storage garlic takes root, this medicament contains juglone, and obtains by the following method: according to (0.1-1) g: the ratio of (5-50) ml takes green peel of walnut and water, is soaked by green peel of walnut 18-32 hour and get final product.
Described juglone also can be adopted and extract with the following method: green peel of walnut powder and ethanol are taken in 1-10g: 4-40mL ratio, be placed in conical flask, sealing, ultrasonic process 20-60min, filters, and filtrate concentrates, decompression and solvent recovery, obtains medicinal extract, is diluted with water to desired concn.
Suppress the preparation method preserving the medicament that garlic takes root, adopt cosolvent to be dissolved by juglone, then add water and obtain the aqueous solution and get final product.
The consumption of described cosolvent is the minimum amount of being dissolved by required juglone.
The method suppressing storage garlic to take root, soaks garlic 3-6 hour by above-mentioned medicament, takes out garlic and dries, naturally place.
The advantage that the present invention has and good effect are: the medicament suppressing storage garlic to take root provided by the invention, juglone raw material sources are wide, both can use the extract of natural juglone plant, and also can use the juglone of chemosynthesis, be easy to preparation, with low cost.Medicament is easy to use, only needs garlic to soak a period of time, both dry can.Juglone consumption is low, and nontoxic, environmental protection, can effectively suppress garlic to root, and can effectively prevent garlic from rotting, inhibitory action is not had to the raw seedling of garlic.
Accompanying drawing explanation
Fig. 1 is green peel of walnut extract and control group to the inhibitory action of preserving garlic and taking root;
Fig. 2 is the inhibitory action that the medicament of juglone extract containing variable concentrations is taken root to storage garlic;
Fig. 3 is the inhibitory action that the juglone containing variable concentrations is taken root to storage garlic;
Fig. 4 is the inhibitory action that the medicament containing different alcohol consumption is taken root to storage garlic.
Embodiment
Embodiment 1:
The medicament suppressing storage garlic to take root, adopts and prepares with the following method: get green peel of walnut 100g, soak 24 hours with 500ml running water, and water intaking immersion liquid is for subsequent use, detects, juglone concentration 0.5% through high pressure liquid phase.
Garlic process: adopt spring from the new outbound garlic of freezer, be divided into control group and processed group at random, often organize 20 garlics, processed group garlic green peel of walnut infusion is soaked 4 hours, and control group garlic is only soaked with running water, takes out garlic and naturally dries, room temperature is placed, and observing maximum duration is 5 weeks.Measure weekly once root length, adopt t-test method to add up the long result of root.
Result of the test: as shown in Figure 1, control group garlic sends out roots after placement for 2 weeks gradually, the process of green peel of walnut infusion has inhibitory action to going out root, only occurred taking root at the 5th week, average root length is far below control group, and all had remarkable statistical discrepancy (p < 0.05) at the 2nd, 3,4,5 week, other glucides contained in green peel of walnut infusion are cosolvent, help solubilization, therefore without the need to adding cosolvent.Other side, the process of green peel of walnut infusion rots there is certain suppression to garlic, does not have inhibitory action to the raw seedling of garlic.
Embodiment 2:
Prepared by juglone extract: accurately take green peel of walnut powder 50g, be placed in 500ml conical flask, accurately add 95% ethanol 200ml, sealing, ultrasonic process 30min (power 40KHz, temperature 25 DEG C), filter, filtrate concentrates, decompression and solvent recovery, obtain medicinal extract and juglone extract, detect through high pressure liquid phase, juglone concentration is 25.2%.
The preparation of the medicament suppressing storage garlic to take root: juglone extract is prepared to obtain five kinds of medicaments with water respectively by juglone concentration 1%, 0.1%, 0.01%, 0.001%, 0.0001%, other glucides also contained in juglone extract are cosolvent, help solubilization, therefore without the need to adding cosolvent.
Garlic process: adopt spring from the new outbound garlic of freezer, be divided into different groups at random, often organize 20 garlics, soak garlic 4 hours with above-mentioned five kinds of medicaments, control group only soaks with running water, naturally dries, and room temperature is placed, and observing maximum duration is 5 weeks.Measure weekly once root length, adopt method of analysis of variance (ANOVA) to add up the long result of root.
Result of the test: result of the test as shown in Figure 2, control group garlic sends out roots after placement for 2 weeks gradually, inhibitory action is had to going out root containing 1%, 0.1%, 0.01%, 0.001% juglone extract-treated, wherein 1%, 0.1% group was showed no and obviously takes root in 5 weeks, 0.01%, take root but occur that comparatively control group is late for 0.001% group, and average length is lower than control group, there is remarkable statistical discrepancy (p < 0.05) at the 5th week.0.001% group to be compared with control group and not to have marked difference.Other side, walnut peel extract process rots there is certain suppression to garlic, does not have inhibitory action to the raw seedling of garlic.
Embodiment 3:
The preparation of the medicament suppressing storage garlic to take root: with Tween 80 by juglone (commercially available chemosynthesis juglone, purity > 99%) dissolve, then water dilution forms five kinds of different medicaments of concentration, and each constituent content is as shown in table 1.
The constituent content of table 1 control group and five kinds of medicaments suppressing storage garlic to take root
Garlic process: adopt from the new outbound garlic of freezer, be divided into different groups at random, often organizes 20 garlics, and adopt above-mentioned medicament to soak garlic 4 hours, control group only soaks with running water, naturally dries, and room temperature is placed, and observing maximum duration is 5 weeks.Measure weekly once root length, adopt method of analysis of variance (ANOVA) to add up the long result of root.
Result of the test: result of the test as shown in Figure 3, control group garlic sends out roots after placement for 2 weeks gradually, 1%, 0.1%, 0.01%, 0.001% juglone process has inhibitory action to going out root, wherein 1%, 0.1% group was showed no and obviously takes root in 5 weeks, 0.01%, 0.001% takes root but occurs that comparatively control group is late, and average length is lower than control group, there is remarkable statistical discrepancy (p < 0.05) at the 5th week.0.0001% group to be compared with control group and not to have marked difference.Other side, juglone process rots there is certain suppression to garlic, does not have inhibitory action to the raw seedling of garlic.Result shows that chemosynthesis juglone and the natural extracts containing juglone have similar suppression garlic rooting effect.
Embodiment 4
The preparation of the medicament suppressing storage garlic to take root: use (95%) alcohol respectively: juglone is 3: 1,2: 1 and 1: 1 volume/mass ratio (ml: g) hydrotropy, then dilute with water juglone forms nine kinds of medicaments that concentration is respectively 1%, 0.1% and 0.01%.
Garlic process: adopt from the new outbound garlic of freezer, be divided into two groups at random, often organizes 20 garlics, and adopt above-mentioned medicament to soak garlic 4 hours, naturally dry, room temperature is placed, and observing maximum duration is 5 weeks.Measure weekly once root length.
Result of the test: as shown in Figure 4, nine kinds of medicaments all have inhibitory action to going out root, be showed no and obviously take root in 5 weeks.Cosolvent alcohol does not all make significant difference to root is long.

Claims (8)

1. suppress the medicament that storage garlic takes root, it is characterized in that, this medicament is the aqueous solution containing juglone and cosolvent, and the concentration of described juglone is 0.001-1%.
2. medicament as claimed in claim 1, the concentration of described juglone is preferably 0.01-1%.
3. medicament as claimed in claim 1, described cosolvent is tween or alcohol.
4. medicament as claimed in claim 1, described cosolvent is Tween 80.
5. suppress the medicament that storage garlic takes root, this medicament contains juglone, and obtains by the following method: according to (0.1-1) g: the ratio of (5-50) ml takes green peel of walnut and water, is soaked by green peel of walnut 18-32 hour and get final product.
6. medicament as claimed in claim 1, described juglone is adopted and is extracted with the following method: green peel of walnut powder and ethanol are taken in 1-10g: 4-40mL ratio, be placed in conical flask, sealing, ultrasonic process 20-60min, filters, filtrate concentrates, decompression and solvent recovery, obtains medicinal extract, is diluted with water to desired concn.
7. the preparation method of the medicament suppressing storage garlic to take root according to claim 1, adopts cosolvent to be dissolved by juglone, then adds water and obtain the aqueous solution.
8. the method suppressing storage garlic to take root, soaks garlic 3-6 hour by medicament according to claim 1, takes out garlic and dries, naturally place.
CN201510275277.XA 2015-05-20 2015-05-20 Medicament for inhibiting root growth of stored garlic Pending CN104855378A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106306002A (en) * 2016-08-24 2017-01-11 韦克康 Garlic retention period extension method
CN106359560A (en) * 2016-08-24 2017-02-01 韦克康 Method of inhibiting odor during eating garlic
CN109566728A (en) * 2018-10-30 2019-04-05 南京林业大学 A kind of complexing agent and its preparation method and application inhibiting the mould rotten germination of garlic

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4895575A (en) * 1982-12-08 1990-01-23 L'oreal Use of 5-hydroxynaphthoquinones for dyeing human keratin fibres
CN1781376A (en) * 2004-12-03 2006-06-07 武汉绿世纪生物工程有限责任公司 Use of walnut plant extract as pesticide in preventing and controlling diamondback moth
CN1836507A (en) * 2006-04-22 2006-09-27 田官荣 Medicine for preventing and treating gadol withered disease
CN102907423A (en) * 2012-10-19 2013-02-06 华南农业大学 Application of juglone to termite control

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4895575A (en) * 1982-12-08 1990-01-23 L'oreal Use of 5-hydroxynaphthoquinones for dyeing human keratin fibres
CN1781376A (en) * 2004-12-03 2006-06-07 武汉绿世纪生物工程有限责任公司 Use of walnut plant extract as pesticide in preventing and controlling diamondback moth
CN1836507A (en) * 2006-04-22 2006-09-27 田官荣 Medicine for preventing and treating gadol withered disease
CN102907423A (en) * 2012-10-19 2013-02-06 华南农业大学 Application of juglone to termite control

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张凤云 等: ""核桃青皮提取物对几种作物幼苗生长的影响"", 《西北农业学报》 *
郝飞 等: ""胡桃醌对小麦种子萌发及幼苗生长的化感效应"", 《西北植物学报》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106306002A (en) * 2016-08-24 2017-01-11 韦克康 Garlic retention period extension method
CN106359560A (en) * 2016-08-24 2017-02-01 韦克康 Method of inhibiting odor during eating garlic
CN109566728A (en) * 2018-10-30 2019-04-05 南京林业大学 A kind of complexing agent and its preparation method and application inhibiting the mould rotten germination of garlic
CN109566728B (en) * 2018-10-30 2022-09-09 南京林业大学 Complexing agent for inhibiting garlic mildew and rot germination and preparation method and application thereof

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