CN1813747A - Perhexiline injection and its preparing method - Google Patents
Perhexiline injection and its preparing method Download PDFInfo
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- CN1813747A CN1813747A CN 200510007261 CN200510007261A CN1813747A CN 1813747 A CN1813747 A CN 1813747A CN 200510007261 CN200510007261 CN 200510007261 CN 200510007261 A CN200510007261 A CN 200510007261A CN 1813747 A CN1813747 A CN 1813747A
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Abstract
The present invention discloses a Guanxinning injection for effectively curing coronary heart disease and ischemic cerebrovascular disease. It is characterized by that said injection is basically formed from tanshinone, salvia phenol acid matter, liguistrazine and ligusticum pherol acid ester matter. Besides, said invention also provides its preparation method by utilizing fresh salvia root and ligusticum root and its concrete steps. It is characterized by that the invented injection not only contains tanshinone II A and ligustrazine, but also contains cryptotanshinone, tanshinone I, salvia phenol acid matter and ligusticum phenol acid ester matter.
Description
Technical field
The present invention relates to a kind of injection for the treatment of coronary heart disease and ischemic cerebrovascular and preparation method thereof, relating in particular to a kind of is that raw material extracts the treatment coronary heart disease be prepared from and the injection of ischemic cerebrovascular with Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong, belongs to the field of Chinese medicines.
Background technology
Treat the injection of coronary heart disease and ischemic cerebrovascular in the market and be made up of Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong two flavor medicines mostly, treatment coronary heart disease and ischemic cerebrovascular determined curative effect are one of clinical injections of Chinese medicine commonly used.The traditional preparation method of this injection is with Radix Salviae Miltiorrhizae, the Rhizoma Chuanxiong decoction pieces mixes and carries out three water and carry, behind the secondary precipitate with ethanol, transfer pH to carry out XINNAONING injection (CN1457784A) this method that acidification obtains being made up of Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong extract and no doubt can make its water solublity of increase behind the salify such as ligustrazine, but ignored main effective ingredient salvianolic acid material in the Radix Salviae Miltiorrhizae, alkannic acid for example, Salvianolic acid A, the salvianolic acid third gradegrade C, under acid condition, can reduce water solublity, even can separate out, cause curative effect to be affected by the prepared injection of this method, carry 3 times with dried decoction pieces water simultaneously, effective ingredient can not extract fully, because the existing defective of above-mentioned preparation method, make the phenolic acid composition in the Radix Salviae Miltiorrhizae, and the compositions such as ligustrazine in the Rhizoma Chuanxiong all can not extract fully, also make simultaneously the phenolic acid Ester in the Rhizoma Chuanxiong, for example ligustilide, the dimerization ligustilide, chrysophanol, caffeic acid, protocatechuic acid, ferulic acids etc. can not extract fully; Reduce the kind and the content of effective ingredient in the injection, directly influenced the therapeutic effect of prepared injection.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and the more definite treatment coronary heart disease of a kind of curative effect and the injection of ischemic cerebrovascular are provided.
The object of the present invention is to provide a kind of injection for the treatment of coronary heart disease and ischemic cerebrovascular, it is characterized in that this injection contains TANSHINONES, salvianolic acid material, ligustrazine as effective ingredient.Preferably, also contain chuanxingol esters of gallic acid material in the injection of the present invention as effective ingredient.
More preferably, the invention provides a kind of injection for the treatment of coronary heart disease and ischemic cerebrovascular, it is characterized in that this injection is by the injection carrier, as distilled water for injection or normal saline, TANSHINONES, salvianolic acid material, ligustrazine, chuanxingol esters of gallic acid material are formed.
TANSHINONES in the injection of the present invention is to be main constituent with the tanshinone, can also comprise one or more cryptotanshinones, Tanshinone I.The salvianolic acid material is to be selected from a kind of in rosmarinic acid, prolithospermic acid, alkannic acid, Salvianolic acid A, the Salvianolic acid C or their combination.Chuanxingol esters of gallic acid material comprises and is selected from a kind of in ligustilide, dimerization ligustilide, chrysophanol, caffeic acid, protocatechuic acid, the ferulic acid or their combination.
Injection of the present invention prepares by following method, this supply comprises 1) fresh Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong are made required decoction pieces through freezing processing, low-temperature vacuum drying respectively, 2) respectively with above-mentioned decoction pieces countercurrent extraction, precipitate with ethanol, hyperfiltration membrane filtration, 3) combining step 2) the gained extracting solution, randomly by chitin modified activated-charcoal column purification; 4) embedding, sterilization.
Particularly, the injection of treatment coronary heart disease of the present invention and ischemic cerebrovascular is to be prepared from by following method:,
1), the preparation of Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong decoction pieces: fresh Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong are thinly sliced, in 2 hours, thin slice slowly is cooled to-24～-32 ℃, continue freezing back and take out alleviation at normal temperatures, low-temperature vacuum drying, water content is reduced to below 5%, and sealing is stored standby
2), salvia piece hot water countercurrent extraction that step 1) is prepared, extracting solution concentrates, add 95% ethanol of 4 times of concentrated solution volumes while stirring in concentrated solution, standing over night precipitates complete after-filtration, and transferring the filtrate pH value is 9, leave standstill, the elimination precipitation is 7 with filtrate pH value readjustment, reclaims ethanol, concentrated solution is standby
3), get and step 2) in heavy Rhizoma Chuanxiong decoction pieces hot water countercurrent extraction such as salvia piece, extracting solution is concentrated, add 95% ethanol of 4 times of concentrated solution volumes while stirring in concentrated solution, standing over night precipitates complete after-filtration, and filtering residue discards, when recovery ethanol is concentrated into 1/4 volume, adjust pH to 3.6,0 ℃ of following cold preservation is spent the night, and filters, filtrate for later use
4), combining step 2) and 3) prepared medicinal liquid, add equivalent water for injection, stir evenly, filter, when heating is concentrated into 1/4 () of cumulative volume, add the activated-charcoal column of depressing by chitin modified, refilter the hyperfiltration film of membrane molecule cutoff value more than 10,000, in filtrate, supply distilled water for injection or normal saline to volume required, embedding, sterilization is promptly.
In the above-mentioned preparation method, in the step 1) fresh Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong are cut into the thin slice of thick 0.3-0.5cm respectively; Low-temperature vacuum drying described in the step 1) is to carry out under the pressure of 10.7-16.0 handkerchief, temperature are lower than 50 ℃ condition; Step 2) with bright Radix Salviae Miltiorrhizaes of 90 ℃ of hot water countercurrent extraction or bright Rhizoma Chuanxiong decoction pieces, the control liquid outlet quantity is bright Radix Salviae Miltiorrhizae or bright Rhizoma Chuanxiong decoction pieces weight 7 times and in the step 3); Step 2) bright Radix Salviae Miltiorrhizae or bright Rhizoma Chuanxiong decoction pieces being concentrated into relative density respectively with the extracting solution of hot water countercurrent extraction gained and in the step 3) is 1.10 (50 ℃ of heat are surveyed); Being filtered into described in the step 3) with 0.4～1.2 μ m filtering with microporous membrane; Being filtered into described in the step 4) used the 0.45um filtering with microporous membrane.
By above-mentioned preparation method, can be to greatest extent extracts active ingredients such as ligustrazine in the TANSHINONES in the Radix Salviae Miltiorrhizae and phenolic acid composition, the Rhizoma Chuanxiong and chuanxingol acid esters be come out, also can effectively remove tannin composition contained in Radix Salviae Miltiorrhizae and the Rhizoma Chuanxiong simultaneously.Adopt the prepared injection of the inventive method compared with similar products, curative effect is more definite, and clinical use is safer reliable.
Further describe preparation method of the present invention and beneficial effect by the following examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit the scope of the invention.
The specific embodiment
[embodiment]
1, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong are respectively after different collecting seasons are gathered, all handle as follows: cut clean reed head, wash crust silt, dry the moisture on surface slightly, be cut into the thin slice of thick 0.3-0.5cm immediately, refrigerating chamber is gone in thin stand sabot, in 2h, slowly be cooled to-24--32 ℃, continue freezing 3h.Take out and to alleviate 3h at normal temperatures, be transferred in the vacuum shelf dryer, be lower than low-temperature vacuum drying under 50 ℃ of conditions, water content is reduced to below 5%, take out the apportion sealing and store standby in pressure, the temperature of 10.7-16.0 handkerchief.
2, get bright Radix Salviae Miltiorrhizae lyophilizing decoction pieces 1000g, extract with 90 ℃ of hot water diafiltrations, control infiltration rate, get percolate 7000ml fully to extracting, heating is concentrated into d=1.10 (50 ℃ of heat are surveyed) and gets concentrated solution 300ml, stir the ethanol that adds 1200ml 95% down, standing over night, on buchner funnel, the elimination precipitation, drip 10%NaOH liquid in pure filtrate, the limit edged stirs to pH=9.Leave standstill 10h, reuse buchner funnel elimination precipitation.Filtrate adds 10%HCL liquid readjustment pH=7, reclaims ethanol, concentrates to such an extent that the 250ml concentrated solution is standby;
3, get bright Rhizoma Chuanxiong lyophilizing decoction pieces 1000g, extract, the control infiltration rate with 90 ℃ of hot water diafiltrations, extremely extract fully, get percolate 7000ml, heating is concentrated into relative density to 1.10 (50 ℃ of heat are surveyed) and gets concentrated solution 100ml, stir the ethanol that adds 400ml 95% down, standing over night is crossed the elimination precipitation on buchner funnel, when being concentrated into 125ml behind the recovery ethanol, drip 10%HCL liquid to pH=3.6,0 ℃ of following cold preservation is spent the night, reuse 1.2um filtering with microporous membrane, and it is standby to get filtrate 125ml;
4, get above-mentioned 2,3 liang of prepared medicinal liquids of step and add 375ml water for injection again after mixing, mixing, when being concentrated into 500ml with 0.45um filtering with microporous membrane post-heating, pressurization is passed through with chitin modified activated-charcoal column, behind hyperfiltration film (the membrane molecule cutoff value is more than 10,000), supply water for injection to 500ml, embedding gets 2ml/ and props up 250, or 10ml/ props up 50, and 100 ℃ of 30min sterilizations promptly.
By gas phase-mass spectrography and assists coating chromatography the preparation-obtained injection of the present invention is analyzed, find, except that containing tanshinone and ligustrazine, also contain cryptotanshinone, Tanshinone I, salvianolic acid material and chuanxingol esters of gallic acid material in the injection of the present invention as the main constituent.Wherein the salvianolic acid material comprises rosmarinic acid, prolithospermic acid, alkannic acid, Salvianolic acid A, Salvianolic acid C; Chuanxingol esters of gallic acid material comprises ligustilide, dimerization ligustilide, chrysophanol, caffeic acid, protocatechuic acid, ferulic acid.And not only tanshinone and ligustrazine content are low in the existing injection, and do not find salvianolic acid material and chuanxingol esters of gallic acid material.
The clinical observation on the therapeutic effect test of [test example] injection of the present invention
One, physical data: accept patient's 62 examples altogether for medical treatment, wherein the oldest person is 82 years old, age reckling 31 years old; Man's 32 examples, women 30 examples; Patients with coronary heart disease 38 examples wherein, paralytic's 6 examples, hyperpietic's 20 examples.
Two, Therapeutic Method: the injection that the intramuscular injection embodiment of the invention is prepared.
Three, efficacy assessment standard:
Produce effects: symptom and sign obviously alleviate.
Effectively: symptom and sign alleviate to some extent.
Invalid: as not reach effective standard.
Three, observation of curative effect result:
The 62 routine patients that accepted for medical treatment, produce effects 45 examples, effective 17 examples, total effective rate is 100%.The clinical observation on the therapeutic effect result of the test shows that injection of the present invention has significant curative effect for coronary heart disease and ischemic cerebrovascular.
Claims (9)
1, a kind of injection for the treatment of coronary heart disease and ischemic cerebrovascular is characterized in that this injection contains TANSHINONES, salvianolic acid material, ligustrazine as active component.
2, according to the injection of claim 1, wherein TANSHINONES is to be selected from a kind of in tanshinone, cryptotanshinone, the Tanshinone I or their combination.
3, according to the injection of claim 1, wherein the salvianolic acid material is to be selected from a kind of in rosmarinic acid, prolithospermic acid, alkannic acid, Salvianolic acid A, the Salvianolic acid C or their combination.
4, according to the injection of claim 1, wherein this injection also contains chuanxingol esters of gallic acid material as active component, and this chuanxingol esters of gallic acid material is to be selected from a kind of in ligustilide, dimerization ligustilide, chrysophanol, caffeic acid, protocatechuic acid, the ferulic acid or their combination.
5, a kind of preparation method for the treatment of coronary heart disease and ischemic cerebrovascular injection, this method may further comprise the steps: 1) fresh Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong are made required decoction pieces through freezing processing, low-temperature vacuum drying respectively, 2) respectively with above-mentioned decoction pieces countercurrent extraction, precipitate with ethanol, hyperfiltration membrane filtration, 3) combining step 2) the gained extracting solution, randomly by chitin modified activated-charcoal column purification, 4) embedding, sterilization.
6, according to the preparation method of claim 5, it is characterized in that: the thin slice that in the step 1) fresh Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong is cut into thick 0.3-0.5cm respectively, in 2 hours, thin slice slowly is cooled to-24～-32 ℃, continue freezing back and take out alleviation at normal temperatures, pressure, temperature at 10.7～16.0 handkerchiefs are lower than vacuum drying under 50 ℃ of conditions, water content is reduced to below 5%, and sealing is stored standby.
7, according to the preparation method of claim 5, it is characterized in that: the salvia piece that step 1) is prepared is with 90 ℃ of hot water countercurrent extraction, and the control liquid outlet quantity is 7 times of bright salvia piece weight, and extracting solution concentrates, 95% ethanol that in concentrated solution, adds 4 times of volumes, leave standstill, filter, transferring the filtrate pH value is 9, leaves standstill again, the elimination precipitation, with filtrate pH value readjustment is 7, reclaims ethanol, and concentrated solution is standby.
8, according to the preparation method of claim 5, it is characterized in that: the Rhizoma Chuanxiong decoction pieces that step 1) is prepared is with 90 ℃ of hot water countercurrent extraction, and the control liquid outlet quantity is 7 times of bright Rhizoma Chuanxiong decoction pieces weight, and extracting solution is concentrated, 95% ethanol that in concentrated solution, adds 4 times of volumes, leave standstill, filter, filtering residue discards, when recovery ethanol is concentrated into 1/4 volume, adjust pH to 3.6,0 ℃ of following cold preservation is spent the night, with 0.4～1.2 μ m filtering with microporous membrane, filtrate for later use.
9, according to the preparation method of claim 5, it is characterized in that: in the step 3) with step 2) after the gained extracting solution merges, add equivalent water for injection, stir evenly, use the 0.45um filtering with microporous membrane, heating is concentrated into 1/4 of cumulative volume, add the activated-charcoal column of depressing by chitin modified, refilter the hyperfiltration film of membrane molecule cutoff value more than 10,000, supply water for injection to volume required, embedding, sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100072617A CN100462077C (en) | 2005-02-06 | 2005-02-06 | Perhexiline injection and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100072617A CN100462077C (en) | 2005-02-06 | 2005-02-06 | Perhexiline injection and its preparing method |
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| CN1813747A true CN1813747A (en) | 2006-08-09 |
| CN100462077C CN100462077C (en) | 2009-02-18 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401844B (en) * | 2008-09-26 | 2011-07-20 | 郭东宇 | Red sage root extract and injection |
| CN106226452A (en) * | 2016-08-29 | 2016-12-14 | 贵州信邦制药股份有限公司 | The discrimination method of Radix Angelicae Sinensis in SHIQUAN DABU JIU |
| CN103735614B (en) * | 2014-01-11 | 2017-12-22 | 西双版纳版纳药业有限责任公司 | The processing method and kidney tea product of a kind of kidney tea raw material |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1555834A (en) * | 2003-12-31 | 2004-12-22 | 广州安健实业发展有限公司 | Medicinal composition containing salvia root effective component and ligusticum effecive component and its preparation |
-
2005
- 2005-02-06 CN CNB2005100072617A patent/CN100462077C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401844B (en) * | 2008-09-26 | 2011-07-20 | 郭东宇 | Red sage root extract and injection |
| CN103735614B (en) * | 2014-01-11 | 2017-12-22 | 西双版纳版纳药业有限责任公司 | The processing method and kidney tea product of a kind of kidney tea raw material |
| CN106226452A (en) * | 2016-08-29 | 2016-12-14 | 贵州信邦制药股份有限公司 | The discrimination method of Radix Angelicae Sinensis in SHIQUAN DABU JIU |
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| CN100462077C (en) | 2009-02-18 |
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Effective date of registration: 20130428 Address after: 036900 Shanxi province Shanyin County Youth Street Zhaohui District No. 2 hospital 5 ranked No. 5 Patentee after: Wang Feng Address before: Xiamen City, Fujian Province, 361012 East Road No. 226, Minmetals building 1718 Patentee before: Guo Dongyu |
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