CN1813518A - Low-temperature sensitive sterile rice fertility conversion monitoring method - Google Patents
Low-temperature sensitive sterile rice fertility conversion monitoring method Download PDFInfo
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- CN1813518A CN1813518A CNA2006100386337A CN200610038633A CN1813518A CN 1813518 A CN1813518 A CN 1813518A CN A2006100386337 A CNA2006100386337 A CN A2006100386337A CN 200610038633 A CN200610038633 A CN 200610038633A CN 1813518 A CN1813518 A CN 1813518A
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Abstract
The present invention discloses a monitoring method of hypothermal sensitive sterile rice fertility conversion. Said method includes the following measures: when the atmospheric temperature is below 24 deg.C, utilizing infrared temperature-measuring instrument or temperature sensor to measure average stem temperature of plants and average atmospheric temperature; when the average stem temperature of plants is less than 22.8 deg.C or average atmospheric temperature is less than 23.1 deg.C, adopting river water whose water temperature is higher than 25 deg.C to make flowing water irrigation to regulate plant temperature or atmospheric temperature and make the average stem temperature of plants be above 22.8 deg.C or make the average atmospheric temperature be above 23.1 deg.C so as to stabilize the sterility of sterile line.
Description
Technical field
The present invention relates to a kind of method for supervising of low-temperature sensitive sterile rice fertility conversion, be applicable to the low-temperature sensitive nuclear sterile rice and do the maternal two-line hybrid rice production of hybrid seeds.
Background technology
China's double-hybrid rice strains research has obtained theory and practice achievement advanced in the world, and to 2005, the accumulative total cultivated area surpassed 8,000,000 hm
2It with the two line method also the making a breakthrough property achievement of super hybridized rice research of technological approaches, by applying unit breed two be the super hybridization rice two line system first reaches the first phase target of Chinese super hybridization rice breeding at home, be honoured with first of China in 2000 science and technology progress top ten news stories.According to incompletely statistics, this combination has added up to plant to exceed 5,000,000 hm
2, be the rice varieties of nearest 4 years domestic year cultivated area maximum.
China is applied at present that the two-line hybrid rice production of hybrid seeds produces temperature-sensitive sterile line and photosensitive-sensitive sterile line two major types.The latter's fertility conversion depends mainly on length at sunshine, and the production of hybrid seeds is safer.The former is restricted by temperature then, and atmospheric temperature is bigger because of the significant monsoon characteristic fluctuation in China rice district, and the male sterile line fertility-sensitive period meets with low temperature can influence seed production purity, and this problem is the hidden danger problem that two-line hybrid rice is produced always.
The short 64S of the maternal training of two line system uses maximum male sterile lines in the two-line hybrid rice breeding, belong to the lower temp-sensing sterile line of fertility conversion origin temp.According to existing research, daily mean temperature is lower than 23.5～24.0 ℃ for three days on end, and training short 64S influences seed purity with the part self-fertility.In area, the middle and lower reach of Yangtze River, there are 2 years in nearest 5 years, 3 light sammings have taken place mid-August be lower than 23.5 ℃ weather continuously, the production of hybrid seeds has produced certain influence to two-line hybrid rice.Nearest 3 years two-line hybrid rice production of hybrid seeds practice shows that low temperature comes interim, is in the seed farm under the same weather condition, because the difference of field microclimates such as landform, ecological water, and seed production purity widely different.Our research proves that also atmospheric temperature influences the short 64S fertility of training by influencing plant temperature.When meeting with the low temperature invasion and attack,, can improve plant temperature, thereby ensure the purity of the two-line hybrid rice production of hybrid seeds if improve the rice field microclimate with the higher relatively irrigation water of temperature.
So far, the fertility conversion estimation of low-temperature sensitive nuclear sterile rice all is to be undertaken by the daily mean temperature that meteorological station is issued, still do not have and monitor correlative study, especially plant body or near the inversion temperature condition it of predicting male sterile line fertility conversion by rice field microclimate and plant temperature and still do not have concrete quantification report.
Summary of the invention
The objective of the invention is in the existing two-line hybrid rice production of hybrid seeds process, fertility-sensitive period meets with the hidden danger problem that abnormal low temperature can endanger seed production purity, a kind of method for supervising to the low-temperature sensitive sterile rice fertility conversion is provided, thereby for adopting ecological measures such as " with the water temperature adjustments " to improve field microclimate, improve plant temperature, provide technical guarantee for stablizing seed production purity.
Purpose purpose of the present invention can reach by following measure:
A kind of method for supervising of low-temperature sensitive sterile rice fertility conversion, it is characterized in that going out in preceding 5～15 days scopes of fringe when the seed farm female parent, atmosphere day, samming was when the microthermal climate below 24 ℃, with infrared radiation thermometer or temperature sensor 02 o'clock every day, 08 o'clock, 14 o'clock, measured in 20 o'clock apart from average stem temperature of ground 15-25 centimetre plant or mean temperature of air, when the average stem temperature of measuring for 4 times of plant is lower than 22.8 ℃ or mean temperature of air and is lower than 23.1 ℃, indication male sterile line fertility will be to educating the direction conversion, the river that can take water temperature to be higher than 25 ℃ carries out the flowing water irrigation practice and regulates plant temperature or temperature to 22.8 ℃ of the average stem temperature of plant or mean temperature of air more than 23.1 ℃, with the sterility of stable sterile line.
Purpose purpose of the present invention can also reach by following measure:
A kind of method for supervising of low-temperature sensitive sterile rice fertility conversion, it is characterized in that going out in preceding 5～15 days scopes of fringe when the seed farm female parent, atmosphere day, samming was when the microthermal climate below 24 ℃, with infrared radiation thermometer or temperature sensor 02 o'clock every day, 08 o'clock, 14 o'clock, measured apart from ground 20 centimetres average stem temperature of plant or mean temperature of air at 20 o'clock, when the average stem temperature of measuring for 4 times of plant is lower than 22.8 ℃ or mean temperature of air and is lower than 23.1 ℃, indication male sterile line fertility will be to educating the direction conversion, the river that can take water temperature to be higher than 25 ℃ carries out the flowing water irrigation practice and regulates plant temperature or temperature to 22.8 ℃ of the average stem temperature of plant or mean temperature of air more than 23.1 ℃, with the sterility of stable sterile line.
Advantage of the present invention:
Present low-temperature sensitive sterile rice fertility conversion forecast is to predict according to the temperature report of meteorological station issue, and the temperature report is to be the base unit issue with the county, does not consider the difference of ecological conditions such as the microclimate of concrete seed farm piece and underlying surface.The present invention by the temperature at direct measurement plant temperature-sensitive position or around it temperature monitor the conversion of male sterile line fertility, considered rice field microclimate and various factors combined influence,, more science more direct, more accurate than atmospheric temperature predicted method to the male sterile line fertility.Can regulate and control immediately also in time to observe directly regulating effect to the microclimate of concrete field piece according to this monitoring result, thereby ensure and raising production of hybrid seeds seed purity, reduce the production of hybrid seeds risk of two-line hybrid rice.
Embodiment
Embodiment 1
18-20 day in August, 2005, Nanjing is attacked by continuous 3 days low temperature, and 3 daily mean temperatures of meteorological observatory's actual measurement are 22.7 ℃, and mean minimum temperature is 21.1 ℃.Before the Jiangsu Province Agriculture Science Institute has 1 mu of two-line hybrid rice newly to make up seed farm (total fertility inversion temperature is 10 of the new combinations of the low temperature sensitive sterile strain production of hybrid seeds below 24 ℃) female parent to be in out fringe 5-10 days.Adopt PTWD-2A type temperature sensor data acquisition unit (TRM-ZS1 type) automatic data collection, 18 be 22.3 ℃ with 20 centimetres the plants stems temperature mean value apart from ground that calculated at 02 o'clock, 08 o'clock, 14 o'clock, 20 o'clock, expectation will influence the fertility and the seed production purity of male sterile line.Reinstate reservoir water from afternoon August 18 and carry out the 15cm irrigation until August 20.The average water temperature of reservoir is 28.9 ℃, and the average water temperature of seed farm water inlet is 28.6 ℃, and the average water temperature of delivery port is 26.1 ℃, makes the average stem temperature of 20cm be increased to 23.6 ℃, and 20cm mean temperature of air is increased to 24.7 ℃.The weather temperature recovers normal after August 21, and 20cm stem temperature is higher than 23.5 ℃.10 of 1 mu of seed farm sterility fertility are all more stable as a result, and pollen fertility and self-fruitful rate are zero, and the combination production of hybrid seeds seed purity of flower synchronization is at 95-98%.
Embodiment 2
3 mu of two-line hybrid rices, two excellent 108 seed farms of applying unit are positioned at rice test field, academy of agricultural sciences, Jiangsu Province.Female parent is trained short 64S for low temperature sensitive sterile strain, and in sowing on June 1, June 25 transplanted.Area, Nanjing in 2005 18-20 day continuous low temperature in August (an average day samming is 21.5-23.5 ℃).This moment, female parent was in out fringe preceding 7 days.Adopt PTWD-2A type temperature sensor data acquisition unit (TRM-ZS1 type) automatic data collection, with the plants stems temperature of measuring apart from ground 15 centimetres at 02 o'clock on the 18th, 08 o'clock, 14 o'clock, 20 o'clock, 4 times mean value is 22.3 ℃, and expectation will influence the fertility and the seed production purity of male sterile line.Adopt 10 centimetres water layer to irrigate, irrigating the river water temperature is 28.9 ℃, and the water inlet water temperature is 28.6 ℃, and the delivery port water temperature is 26.0 ℃, makes the average stem temperature of 15cm height plant be increased to 23.5 ℃.Use the method for three days on end, up to August 20.The weather temperature recovers normal after August 21, and 15cm stem temperature is higher than 23.5 ℃.The seed farm female parent is in 25-30 sunrise in August fringe as a result, this flower synchronization of father and mother, and hybrid seed yield is amounted to 124 kilograms/mu, and purity is 98.2%.
Embodiment 3
2005, the 1 mu of short 64S of training experimental field was planted by applying unit, is provided with moistening irrigation (contrast) and deep water purling (processing) dual mode.During fertility-sensitive period is subjected to 18-August 26 August, have 7 days and be lower than 24 ℃ of low temperature harm.Adopt PTWD-2A type temperature sensor with per 30 seconds automatic data collections of data acquisition unit (TRM-ZS1 type), calculate 20 centimetres the gentle temperature of measuring 02 o'clock every day,,, of plants stems at 08 o'clock at 14 o'clock at 20 o'clock apart from ground.Measurement result proves that the contrast field gentle mean temperature of air of the average stem of 7 days 20cm plant is respectively 22.9 ℃ and 22.5 ℃, and adopts the gentle mean temperature of air of average stem of the processing field 20cm of flowing water irrigation to be respectively 23.3 ℃ and 24.6 ℃.The average fertile flower powder percentage in contrast and processing field is respectively 17.76% and 0.39%, and the average inbreeding ripening rate is respectively 5.23% and 0.13%.Contrast field male sterile line fertility has been converted to and can have educated, and still keeps its sterility (self-fruitful rate is lower than 0.5%) and handle the field male sterile line.
Embodiment 4
2004 year-climate conditions are more superior, are lower than 24 ℃ of weather that are unfavorable for the two-line hybrid rice production of hybrid seeds and appear at early September.The mean temperature of air in September 8 to September 10 is 22.1 ℃, and the minimum air temperature is 19.4 ℃.In the short 64S of the training of applying unit experimental field (1.6 mu of areas), 02,08,14,20: 4 average stem temperature of 25cm that adopt PTWD-2A type temperature sensor monitors to arrive are 21.5 ℃.Go here and there continuously with the river of the 10-15cm degree of depth and to irritate training short 64S experimental field (processings), the gentle temperature comparison of 25cm stem is shone (not pouring water) and has not been improved 1.4 ℃ and 2.2 ℃ respectively, reaches 22.9 ℃ and 24.3 ℃ respectively.Train short 64S and be treated to 0.19% in the bagging self-fruitful rate irrigation of heading mid-September, sterility is normal, and contrast is 2.44%, has been converted to and can have educated.
Claims (2)
1, a kind of method for supervising of low-temperature sensitive sterile rice fertility conversion, it is characterized in that going out in preceding 5～15 days scopes of fringe when the seed farm female parent, atmosphere day, samming was when the microthermal climate below 24 ℃, with infrared radiation thermometer or temperature sensor 02 o'clock every day, 08 o'clock, 14 o'clock, measured in 20 o'clock apart from average stem temperature of ground 15-25 centimetre plant or mean temperature of air, when the average stem temperature of measuring for 4 times of plant is lower than 22.8 ℃ or mean temperature of air and is lower than 23.1 ℃, indication male sterile line fertility will be to educating the direction conversion, the river that can take water temperature to be higher than 25 ℃ carries out the flowing water irrigation practice and regulates plant temperature or temperature to 22.8 ℃ of the average stem temperature of plant or mean temperature of air more than 23.1 ℃, with the sterility of stable sterile line.
2, the method for supervising of low-temperature sensitive sterile rice fertility conversion according to claim 1, it is characterized in that going out in preceding 5～15 days scopes of fringe when the seed farm female parent, atmosphere day, samming was when the microthermal climate below 24 ℃, with infrared radiation thermometer or temperature sensor 02 o'clock every day, 08 o'clock, 14 o'clock, measured apart from ground 20 centimetres average stem temperature of plant or mean temperature of air at 20 o'clock, when the average stem temperature of measuring for 4 times of plant is lower than 22.8 ℃ or mean temperature of air and is lower than 23.1 ℃, indication male sterile line fertility will be to educating the direction conversion, the river that can take water temperature to be higher than 25 ℃ carries out the flowing water irrigation practice and regulates plant temperature or temperature to 22.8 ℃ of the average stem temperature of plant or mean temperature of air more than 23.1 ℃, with the sterility of stable sterile line.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009901A (en) * | 2015-07-23 | 2015-11-04 | 安徽省农业科学院水稻研究所 | Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method |
| CN111602592A (en) * | 2020-05-25 | 2020-09-01 | 湖南师范大学 | Breeding method and application of rice low temperature sensitive genic male sterile line with high sterility critical temperature of high and low temperature resistance |
| DE202022102325U1 (en) | 2022-04-29 | 2022-05-11 | Syed Rehan Ahmad | Paddy monitoring system with IoT and smart circuits for agriculture based high financial results |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
-
2006
- 2006-03-06 CN CNA2006100386337A patent/CN1813518A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105009901A (en) * | 2015-07-23 | 2015-11-04 | 安徽省农业科学院水稻研究所 | Method for detecting sterility of photo-thermo-sensitive genic male sterile rice according to multiphase and multi-sample method |
| CN111602592A (en) * | 2020-05-25 | 2020-09-01 | 湖南师范大学 | Breeding method and application of rice low temperature sensitive genic male sterile line with high sterility critical temperature of high and low temperature resistance |
| CN111602592B (en) * | 2020-05-25 | 2021-11-16 | 湖南师范大学 | Breeding method and application of rice low temperature sensitive genic male sterile line with high sterility critical temperature of high and low temperature resistance |
| CN114830936A (en) * | 2022-04-24 | 2022-08-02 | 河南师范大学 | Method for thoroughly sterilizing pollen of different fertility groups of BNS series temperature-sensitive genic sterile wheat |
| DE202022102325U1 (en) | 2022-04-29 | 2022-05-11 | Syed Rehan Ahmad | Paddy monitoring system with IoT and smart circuits for agriculture based high financial results |
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