CN1788795A - Carbon dioxide pressurized sterilization method under normal temperature - Google Patents
Carbon dioxide pressurized sterilization method under normal temperature Download PDFInfo
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- CN1788795A CN1788795A CN 200510061997 CN200510061997A CN1788795A CN 1788795 A CN1788795 A CN 1788795A CN 200510061997 CN200510061997 CN 200510061997 CN 200510061997 A CN200510061997 A CN 200510061997A CN 1788795 A CN1788795 A CN 1788795A
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- carbon dioxide
- autoclave
- sterilization
- normal temperature
- viable bacteria
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 48
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 title claims description 122
- 229910002092 carbon dioxide Inorganic materials 0.000 title claims description 61
- 239000001569 carbon dioxide Substances 0.000 title claims description 61
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 59
- 230000000694 effects Effects 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 4
- 108090000623 proteins and genes Proteins 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 abstract description 4
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 239000000796 flavoring agent Substances 0.000 abstract description 3
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 230000007062 hydrolysis Effects 0.000 abstract description 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 2
- 241000235342 Saccharomycetes Species 0.000 abstract 1
- 230000009849 deactivation Effects 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 26
- 238000013019 agitation Methods 0.000 description 14
- 239000001888 Peptone Substances 0.000 description 13
- 108010080698 Peptones Proteins 0.000 description 13
- 235000019319 peptone Nutrition 0.000 description 13
- 239000011780 sodium chloride Substances 0.000 description 13
- 239000000725 suspension Substances 0.000 description 13
- 241000588724 Escherichia coli Species 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 230000002779 inactivation Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 241000293029 Absidia caerulea Species 0.000 description 3
- 240000001929 Lactobacillus brevis Species 0.000 description 3
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000001665 lethal effect Effects 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 235000013405 beer Nutrition 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000002498 deadly effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses normal temperature pressurizing CO2 sterilization method. The sterilization method includes introducing CO2 to eliminate residual air from autoclave, filling liquid to be sterilized to at most 75 vol% of the autoclave, balancing the temperature of the autoclave in water bath at 25-45 deg.c for 10-15 min, introducing CO2 at 2-8 MPa and 25-45 deg.c, and maintaining the pressure for 5-120 min. The present invention has broad spectrum sterilization of killing Gram+ bacterium, Gram- bacterium, saccharomycetes, mildew and other microbes. The normal temperature operation is suitable for sterilizing heat sensitive matter and maintaining food flavor, and lowering pressure while prolonging the pressure maintaining period can reduce the deactivation and hydrolysis of enzyme, protein and other matters. Small amount of alcohol may be added to raise the sterilization effect, save power and reduce operation period.
Description
Technical field
The present invention relates to a kind of carbon dioxide pressurized sterilization method under normal temperature.
Background technology
In food industry and biological technical field, usually need complex is carried out sterilization treatment.The most frequently used sterilizing methods is a heat sterilization now, and the maximum deficiency of this method is easily to make thermo-labile material inactivations such as protein, aminoacid and enzyme, also influences some flavours in food products, therefore needs exploitation room temperature sterilizing methods.Room temperature sterilizing methods existing now or that developing has ultraviolet sterilization, chemical sterilization, supertension sterilize method, radiation or high energy electron sterilization, oscillating magnetic field sterilization etc.Kamihira etc. find that in 1987 the carbon dioxide under the certain pressure has the phenomenon that makes the lethal effect of certain micro-organisms, utilize this phenomenon, and making to adopt carbon dioxide pressurizedly becomes possibility as the room temperature sterilizing methods.Can to play the effect of the growth and breeding that suppresses aerobic microbiological different with adding non-pressurized carbon dioxide for this principle, also make the lethal principle of microorganism different with the supertension sterilize method that needs the hundreds of MPa, and carbon dioxide pressurized have a special mechanism of action.
According to our research and relevant report, carbon dioxide pressurized deadly mechanism to microorganism causes the intracellular organic matter inactivation and runs off relevant with carbon dioxide enrichment in cell.Very little at the dissolubility of normal pressure carbon dioxide in solution, increase along with system pressure, the carbon dioxide dissolubility increases sharply, after entering liquid phase, by transmitting, enter cell by cell membrane again, and be enriched in the cell near cell, after carbon dioxide reached finite concentration in the cell, cell began death.Carbon dioxide pressurized meeting causes cell membrane to be subjected to certain damage, and intracellular organic matter leaks on a small quantity, the ability of the inside and outside pH value of cell membrane disequilibrium cell; After the accumulation carbon dioxide surpasses the cell buffer capacity in cell, cause that pH reduces in the born of the same parents, active substance inactivation in the born of the same parents, cell thereby can't carry out normal physiological activity and death.
Adopt the carbon dioxide pressurized research that does not have at home so far to be correlated with as the room temperature sterilizing methods to report.Carbon dioxide pressurized simple, easy to operate as the required device of sterilizing methods, the primary raw material carbon dioxide is easy to get, and mild condition is good to the lethal effect of microorganism, can be used as effectively replenishing of heat sterilization method.
Summary of the invention
The purpose of this invention is to provide a kind of carbon dioxide pressurized sterilization method under normal temperature.
Carbon dioxide pressurized sterilization method under normal temperature is to feed carbon dioxide to drive the interior residual air of autoclave away, again liquid subject to sterilization is added in the still, liquid addition subject to sterilization is no more than 75% of autoclave volume, autoclave is balance 10-15min in the water-bath of 25-45 ℃ of temperature, in autoclave, feed 2-8MPa then, 25-45 ℃ carbon dioxide, and got final product in pressurize 5-120 minute.
Add the magnetic force rotor in autoclave, the rotating speed of the magnetic stirring apparatus that autoclave is outer is 100-300rpm.The ethanol that adds mass percent in the liquid subject to sterilization and be 2-10% is as secondary solvent.
The advantage of this method is
1) sterilization scope is wide, can effectively kill Gram
+Antibacterial, Gram
-Multiple microorganisms such as antibacterial, yeast and mycete;
2) room temperature operation down is applicable to the sterilization of heat-sensitive substance, and is easy to keep flavour of food products;
3) reduce pressure, prolong the dwell time, can under the prerequisite that guarantees sterilization effect, reduce the inactivation hydrolysis of materials such as enzyme and protein as far as possible;
4) stir by adding, add small amount of ethanol, can improve carbon dioxide pressurized sterilization effect, save the energy and operating time, ethanol is nontoxic and volatile;
5) equipment is simple, and is easy to operate, and mild condition is easy to promote.
Description of drawings
Accompanying drawing is a carbon dioxide pressurized sterilization device of the present invention.
The specific embodiment
As shown in drawings, room temperature carbon dioxide pressurized sterilization device is connected with carbon dioxide steel cylinder 1, plunger displacement pump 2, gas preheater 3, autoclave 4, discharging opening 10 in turn, autoclave 4 is provided with precision pressure gauge 9, charging aperture 7, high-order feed well 8, atmospheric valve 11, and autoclave 4 peripheral hardwares have water bath with thermostatic control 5, magnetic stirring apparatus 6.
1. room temperature descends carbon dioxide pressurized sterilization under the no stirring condition
Embodiment 1
Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, feed pressurize behind 7.8Mpa, 35 ℃ the carbon dioxide.Drop to 10 through viable bacteria rate behind the 15min (microbe quantity before viable bacteria rate=processing back microbe quantity/processing)
-1, be 10 through viable bacteria rate behind the 30min
-2.4, be 10 through viable bacteria rate behind the 60min
-4.2
2. adopt the carbon dioxide pressurized sterilization of 180rpm stirring
Embodiment 2
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-1.8, be 10 through viable bacteria rate behind the 30min
-4.1, be 10 through viable bacteria rate behind the 60min
-7.8Comparing embodiment 1 and embodiment 2 adopt as can be seen to stir and can improve carbon dioxide pressurized sterilization effect.
Embodiment 3
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 4.9MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-0.4, be 10 through viable bacteria rate behind the 30min
-2.0, be 10 through viable bacteria rate behind the 60min
-5.1, be 10 through viable bacteria rate behind the 90min
-7.6
Embodiment 4
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 2.0MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-0.1, be 10 through viable bacteria rate behind the 30min
-0.8, be 10 through viable bacteria rate behind the 60min
-2.5, be 10 through viable bacteria rate behind the 90min
-3.9Comparing embodiment 2, embodiment 3 and embodiment 4, the sterilization effect that the favourable enhancing of higher as can be seen pressure is carbon dioxide pressurized.
Embodiment 5
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 45 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 45 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-2.6, be 10 through viable bacteria rate behind the 30min
-5.3, be 10 through viable bacteria rate behind the 60min
-8.3
Embodiment 6
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 25 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 25 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-1.2, be 10 through viable bacteria rate behind the 30min
-3.0, be 10 through viable bacteria rate behind the 60min
-5.3Comparing embodiment 2, embodiment 5 and embodiment 6, higher as can be seen temperature helps improving carbon dioxide pressurized sterilization effect.
Embodiment 7
Fresh Lactobacillus brevis is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 30min is 10
-3.7, be 10 through viable bacteria rate behind the 60min
-7.3
Embodiment 8
Fresh Saccaromyces cerevisiae is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 30min is 10
-4.6, be 10 through viable bacteria rate behind the 60min
-8.8
Embodiment 9
Fresh Absidia coerulea spore is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 30min is 10
-2.1, be 10 through viable bacteria rate behind the 60min
-5.2
3. add the carbon dioxide pressurized sterilization of ethanol as entrainer
Embodiment 10
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, adding ethanol makes its concentration reach 10% (mass percent), mix, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize 5min behind carbon dioxide certain pressure, 35 ℃.Through viable bacteria rate after the carbon dioxide treatment of 4.9MPa pressure is 10
-8.1, be 10 through viable bacteria rate after the carbon dioxide treatment of 2.0MPa pressure
-3.63
Embodiment 11
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, adding ethanol makes its concentration reach 5% (mass percent), mix, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize 5min behind carbon dioxide certain pressure, 35 ℃.Through viable bacteria rate after the carbon dioxide treatment of 7.8MPa pressure is 10
-8.4, be 10 through viable bacteria rate after the carbon dioxide treatment of 4.9MPa pressure
-4.7, be 10 through viable bacteria rate after the carbon dioxide treatment of 2.0MPa pressure
-0.6
Embodiment 12
Fresh Escherichia coli is suspended in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone makes bacteria suspension, adding ethanol makes its concentration reach 2% (mass percent), mix, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize 5min behind carbon dioxide certain pressure, 35 ℃.Through viable bacteria rate after the carbon dioxide treatment of 7.8MPa pressure is 10
-3.3, be 10 through viable bacteria rate after the carbon dioxide treatment of 4.9MPa pressure
-2.0, be 10 through viable bacteria rate after the carbon dioxide treatment of 2.0MPa pressure
-0.2Comparing embodiment 2, embodiment 10, embodiment 11 and embodiment 12 add a spot of ethanol as can be seen and can improve sterilization effect greatly, and can reduce the operating pressure of carbon dioxide pressurized sterilization, shorten the dwell time; The amount of alcohol that adds is many more, and the effect of sterilization is also good more.
Embodiment 13
Fresh Lactobacillus brevis, Saccaromyces cerevisiae and Absidia coerulea be suspended in respectively in the solution that contains 0.85% (mass percent) NaCl and 0.1% (mass percent) peptone make bacteria suspension, adding ethanol makes its concentration reach 5% (mass percent), mix, place in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize 5min behind 7.8MPa, 35 ℃ the carbon dioxide.The viable bacteria rate of Lactobacillus brevis is 10
-7.5, the viable bacteria rate of Saccaromycescerevisiae is 10
-7.4, the viable bacteria rate of Absidia coerulea is 10
-4.4
4. adopt carbon dioxide pressurized to milk and medicated beer sterilization
Embodiment 14
Fresh Escherichia coli is suspended in Erie's plain chocolate, places then in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 7.8MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 15min is 10
-0.1, be 10 through viable bacteria rate behind the 30min
-2.6, be 10 through viable bacteria rate behind the 60min
-4.9, be 10 through viable bacteria rate behind the 120min
-7.5, this moment, the protein loss of milk was no more than 20%.
Embodiment 15
Fresh Saccaromyces cerevisiae is suspended in the board medicated beer of money river, places then in the autoclave, adjust temperature in the kettle to 35 ℃, the magnetic agitation rotating speed is 180rpm, feeds pressurize behind 4.9MPa, 35 ℃ the carbon dioxide.Through viable bacteria rate behind the 5min is 10
-5.9, through viable bacteria rate＜10 behind the 10min
-9
Claims (3)
1. carbon dioxide pressurized sterilization method under normal temperature, it is characterized in that, feed carbon dioxide and drive the interior residual air of autoclave away, again liquid subject to sterilization is added in the still, liquid addition subject to sterilization is no more than 75% of autoclave volume, and autoclave in 25-45 ℃ water-bath balance 10-15 minute feeds 2-8MPa pressure then in autoclave, the carbon dioxide of 25-45 ℃ of temperature, and pressurize 5-120min gets final product.
2. a kind of carbon dioxide pressurized sterilization method under normal temperature according to claim 1 is characterized in that adding the magnetic force rotor in the described autoclave, and the rotating speed of the magnetic stirring apparatus that autoclave is outer is 100-300rpm.
3. a kind of carbon dioxide pressurized sterilization method under normal temperature according to claim 1, the ethanol that it is characterized in that in the described liquid subject to sterilization adding mass percent and be 2-10% is as secondary solvent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100619972A CN1325120C (en) | 2005-12-14 | 2005-12-14 | Carbon dioxide pressurized sterilization method under normal temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100619972A CN1325120C (en) | 2005-12-14 | 2005-12-14 | Carbon dioxide pressurized sterilization method under normal temperature |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1788795A true CN1788795A (en) | 2006-06-21 |
| CN1325120C CN1325120C (en) | 2007-07-11 |
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|---|---|---|---|
| CNB2005100619972A Expired - Fee Related CN1325120C (en) | 2005-12-14 | 2005-12-14 | Carbon dioxide pressurized sterilization method under normal temperature |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579531B (en) * | 2009-06-09 | 2013-03-06 | 广东省农业科学院蚕业与农产品加工研究所 | Method for three-phase sterilization of liquid material and equipment thereof |
| CN103932349A (en) * | 2014-04-11 | 2014-07-23 | 西安因诺生物工程科技有限公司 | Carbon dioxide-synergized high-pressure non-thermal sterilization method and sterilization system of liquid material |
| CN109924389A (en) * | 2019-03-27 | 2019-06-25 | 贵州红星山海生物科技有限责任公司 | A kind of low temperature sterilization method of capsochrome |
| CN113995870A (en) * | 2021-12-30 | 2022-02-01 | 中国农业大学 | Method for killing coronavirus on surface of outer packaging material packaged with cold chain product |
| CN115607701A (en) * | 2022-11-10 | 2023-01-17 | 江门华大生物科技有限公司 | Irradiation sterilization method of porous interbody fusion cage |
| CN115669730A (en) * | 2022-10-31 | 2023-02-03 | 黑龙江辰鹰乳业有限公司 | Formula liquid milk suitable for 0-6 month babies and preparation method thereof |
-
2005
- 2005-12-14 CN CNB2005100619972A patent/CN1325120C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101579531B (en) * | 2009-06-09 | 2013-03-06 | 广东省农业科学院蚕业与农产品加工研究所 | Method for three-phase sterilization of liquid material and equipment thereof |
| CN103932349A (en) * | 2014-04-11 | 2014-07-23 | 西安因诺生物工程科技有限公司 | Carbon dioxide-synergized high-pressure non-thermal sterilization method and sterilization system of liquid material |
| CN103932349B (en) * | 2014-04-11 | 2016-03-30 | 西安因诺生物工程科技有限公司 | A kind of carbon dioxide of liquid material works in coordination with high pressure nonthermal method and disinfection system |
| CN109924389A (en) * | 2019-03-27 | 2019-06-25 | 贵州红星山海生物科技有限责任公司 | A kind of low temperature sterilization method of capsochrome |
| CN113995870A (en) * | 2021-12-30 | 2022-02-01 | 中国农业大学 | Method for killing coronavirus on surface of outer packaging material packaged with cold chain product |
| CN115669730A (en) * | 2022-10-31 | 2023-02-03 | 黑龙江辰鹰乳业有限公司 | Formula liquid milk suitable for 0-6 month babies and preparation method thereof |
| CN115607701A (en) * | 2022-11-10 | 2023-01-17 | 江门华大生物科技有限公司 | Irradiation sterilization method of porous interbody fusion cage |
| CN115607701B (en) * | 2022-11-10 | 2024-03-22 | 江门华大生物科技有限公司 | Irradiation sterilization method of porous interbody fusion cage |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1325120C (en) | 2007-07-11 |
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