CN113995870A - Method for killing coronavirus on surface of outer packaging material packaged with cold chain product - Google Patents
Method for killing coronavirus on surface of outer packaging material packaged with cold chain product Download PDFInfo
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- CN113995870A CN113995870A CN202111636656.9A CN202111636656A CN113995870A CN 113995870 A CN113995870 A CN 113995870A CN 202111636656 A CN202111636656 A CN 202111636656A CN 113995870 A CN113995870 A CN 113995870A
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- killing
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- packaging material
- carbon dioxide
- cold chain
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/36—Freezing; Subsequent thawing; Cooling
- A23L3/37—Freezing; Subsequent thawing; Cooling with addition of or treatment with chemicals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
Abstract
The invention provides a method for killing coronavirus on the surface of an outer packaging material packaged with a cold chain product, which comprises the following steps: a first viral killing treatment comprising applying an ethanol solution to an overwrapping material encapsulating a cold chain product; a second viral eradication process, the second viral eradication process including: placing the packaging material subjected to the first virus killing treatment in a closed killing bin, controlling the temperature in the killing bin and keeping the killing bin in a vacuum state, and filling liquid carbon dioxide into the killing bin to immerse the packaging material in the liquid carbon dioxide; the temperature in the sterilizing bin is controlled to be 6-12 ℃, and the temperature of the liquid carbon dioxide is controlled to be 6-12 ℃. The method can efficiently kill the coronavirus in the cold chain product outer packaging bag, and simultaneously maintain the quality of the cold chain product to the maximum extent.
Description
Technical Field
The invention relates to the field of food biology. In particular, the present invention relates to a method of killing coronavirus on the surface of a packaging material enclosing a cold chain product.
Background
The detection of new coronavirus positive on the surface of the outer package of the imported cold chain product requires the establishment of a human-like disease prevention and control system. The existing disinfection method mainly adopts a chemical disinfection method, but has a plurality of defects, and the disinfection effect cannot be ensured. Chemical disinfectants treat cold chain food packaging faces a number of new problems of applicability: health risks of long-term exposure to field personnel; the manual disinfection efficiency is low, the operation is not standard, the disinfection effect is difficult to ensure, and the supervision pressure is high; the sterilizing ability of the chemical disinfectant on the surface at low temperature is reduced; the paper packing box is affected with damp and cracked, increases the food sanitation pollution risk and seriously affects the product sale.
High Pressure Carbon Dioxide (HPCD) is a non-thermal processing technology that uses the high acid and anaerobic environment formed by carbon dioxide under a certain pressure (usually 5-50 MPa) and at a lower temperature (usually 0-60 ℃) to assist in a rapid pressure rise and drop process to achieve microbial killing and enzyme inactivation. At present, no research reports that HPCD has a killing effect on viruses, and the research is still needed.
Disclosure of Invention
The present invention aims to solve at least to some extent at least one of the technical problems of the prior art. Therefore, the invention provides a method for killing coronavirus on the surface of an outer packaging material packaged with a cold chain product, which can kill the coronavirus on an outer packaging bag of the cold chain product with high efficiency and keep the quality of the cold chain product to the maximum extent.
In one aspect of the invention, a method of destroying coronavirus on a surface of an outer packaging material enclosing a cold chain product is provided. According to an embodiment of the invention, the method comprises: a first viral killing treatment comprising applying an ethanol solution to an overwrapping material encapsulating a cold chain product; a second viral eradication process, the second viral eradication process including: placing the packaging material (containing the packaged cold chain product) subjected to the first virus killing treatment in a closed killing bin, controlling the temperature in the killing bin and keeping the killing bin in a vacuum state, and filling liquid carbon dioxide into the killing bin to immerse the packaging material in the liquid carbon dioxide; the temperature in the sterilizing bin is controlled to be 6-12 ℃, and the temperature of the liquid carbon dioxide is controlled to be 6-12 ℃.
The inventor finds that the liquid carbon dioxide sterilization mode does not have a good effect on killing the coronavirus in any existing mode, for example, the effect on killing the coronavirus on the surface of the cold chain food is not obvious, and the effect on killing the coronavirus on the surface of the outer packaging material of the cold chain product is relatively high. Meanwhile, the traditional ethanol virus killing mode is adopted to kill the external packaging material, the effect is not obvious, but the traditional ethanol virus killing mode is combined with the liquid carbon dioxide virus killing mode, so that the unexpected better effect is achieved, and the coronavirus on the surface of the external packaging material can be remarkably killed.
Further, the inventor researches the virus killing temperature of the liquid carbon dioxide, and finds that if the temperature is too high, the cold chain product is obviously thawed, and further the quality is reduced due to cold chain food juice loss, repeated freeze thawing and the like; the virus killing effect is weakened due to the fact that the temperature is too low, the frozen product is kept in a good frozen state due to the fact that the temperature is too low, water activity is low, and carbon dioxide cannot enter microbial cells to be mechanically damaged and dissolved and acidified. Namely, low temperature carbon dioxide kill of cold chain products requires a balance to be found between avoiding quality damage due to significant thawing and ensuring virus kill with slight surface thawing. When the temperature is 6-12 ℃, the quality of the cold chain food can be maintained to the maximum extent while the microorganisms on the surface of the outer packaging material are killed.
According to an embodiment of the invention, the method for killing coronavirus on the surface of an outer packaging material enclosing a cold chain product may further have the following additional technical features:
according to the embodiment of the invention, the pressure of the liquid carbon dioxide is 5-10 MPa. Under such conditions, coronavirus on the outer packaging material can be effectively killed.
According to the embodiment of the invention, the immersion time is 10-20 minutes. Under the condition, the coronavirus on the outer packaging material can be effectively killed, and the quality of the cold chain product can be kept to the maximum extent.
According to an embodiment of the present invention, the concentration of the ethanol solution is 1 to 5 vol%. Therefore, the coronavirus killing effect is better.
According to an embodiment of the invention, the coronavirus is human seasonal coronavirus hCoV-229E.
According to an embodiment of the invention, the outer packaging material is polyethylene.
In another aspect of the invention, the invention provides a method of killing human seasonal coronavirus hCoV-229E on the surface of a polyethylene overwrapper. According to an embodiment of the invention, the method comprises: coating 1 volume percent of ethanol solution on the surface of a polyethylene outer packaging material packaged with a cold chain product; and (3) placing the outer packaging material treated in the previous step into a closed sterilizing bin, controlling the temperature in the sterilizing bin to be 10 ℃ and keeping the sterilizing bin in a vacuum state, and filling liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa into the sterilizing bin to immerse the outer packaging material into the liquid carbon dioxide for 15 minutes. Therefore, the method provided by the embodiment of the invention can kill the coronavirus on the cold chain product outer packaging bag with high efficiency, and simultaneously, the quality of the cold chain product is kept to the maximum extent.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram showing the analysis of human seasonal coronavirus titer on the surface of polyethylene packaging material after treatment of control group and experimental group 1-4 according to an embodiment of the invention;
FIG. 2 shows a schematic representation of the analysis of human seasonal coronavirus titres from salmon surfaces after treatment in Experimental group 5 according to one embodiment of the present invention;
figure 3 shows a schematic of an analysis of human seasonal coronavirus titer of shrimp shell surfaces after experimental group 6 treatment according to one embodiment of the invention.
Detailed Description
The scheme of the invention will be explained with reference to the examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1
In the embodiment, the human seasonal coronavirus hCoV-229E on the surface of the polyethylene outer packaging material of the cold chain food is killed in the following way, and the specific steps are as follows:
control group: the outer wrapper is not treated.
Experimental group 1: placing the outer packaging material with the frozen salmon at 10 deg.C for 15 min
Experimental group 2: the outer packaging material packaged with the frozen salmon is placed in a closed killing bin, the temperature in the killing bin is controlled to be 10 ℃ and is in a vacuum state, liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa is filled into the killing bin, so that the outer packaging material is immersed in the liquid carbon dioxide and is maintained for 15 minutes.
Experimental group 3: a 1 vol% ethanol solution was applied to the surface of the outer packaging material in which the frozen salmon was packaged, and then allowed to stand at 10 ℃ for 15 minutes.
Experimental group 4: coating 1 volume percent ethanol solution on the surface of an outer packaging material packaged with the frozen salmon, then placing the outer packaging material in a closed killing bin, controlling the temperature in the killing bin to be 10 ℃ and keeping the outer packaging material in a vacuum state, filling liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa into the killing bin, and immersing the outer packaging material in the liquid carbon dioxide for 15 minutes.
Experimental group 5: the frozen salmon is placed in a closed killing bin, the temperature in the killing bin is controlled to be 10 ℃ and is in a vacuum state, liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa is filled into the killing bin, so that the outer packaging material is immersed in the liquid carbon dioxide and is maintained for 15 minutes.
Experimental group 6: the frozen shrimp shells are placed in a closed sterilizing bin, the temperature in the sterilizing bin is controlled to be 10 ℃ and is in a vacuum state, liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa is filled into the sterilizing bin, so that the outer packaging material is immersed in the liquid carbon dioxide and is maintained for 15 minutes.
Detecting the titer of human seasonal coronavirus on the surfaces of the treated outer packaging materials of the experimental groups 1-4 and the control group, the surface of the treated frozen salmon of the experimental group 5 and the surface of the treated shrimp shell of the experimental group 6, and performing 10-fold serial dilution by using virus diluent (containing 2% FBS, double antibody and mycoplasma inhibitor); 96-well cell plates were washed 2 times with PBS; adding virus dilutions of different dilutions into the cell wells in the order of high dilution → low dilution, 6 replicates per dilution, simultaneously setting up cell control, placing in incubator to 120hpi, observing CPE at 120hpi, recording and calculating TCID50。
As shown in FIG. 1, the effect of the low temperature treatment at 10 ℃ alone (test group 1), the HPCD treatment alone (test group 2) and the ethanol treatment at 1% concentration alone (test group 3) was compared with that of the control group, and the virus titer was statistically insignificant. The effect of 1.0% ethanol and HPCD co-treatment (experimental group 4) was compared with that of untreated group (control group), 10 ℃ low temperature treatment alone (experimental group 1), HPCD treatment alone (experimental group 2), and 1.0% ethanol treatment alone (experimental group 3), and the virus titer was significantly different by statistical analysis. Therefore, the synergistic treatment of ethanol and HPCD with the concentration of 1.0% has better virus killing effect.
As shown in FIGS. 2 and 3, the treatment effect of the experimental group 5 and the experimental group 6 was compared with that of the control group, and the virus titer was statistically insignificant. Therefore, the HPCD does not have a good sterilization effect on viruses in any environment, the virus sterilization effect on the surface of the frozen food is poor, and the virus sterilization effect on the surface of the outer packaging material is good.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (7)
1. A method of destroying coronavirus on a surface of an overwrapping material enclosing a cold chain product, comprising:
a first viral killing treatment comprising applying an ethanol solution to an overwrapping material encapsulating a cold chain product;
a second viral eradication process, the second viral eradication process including: placing the packaging material subjected to the first virus killing treatment in a closed killing bin, controlling the temperature in the killing bin and keeping the killing bin in a vacuum state, and filling liquid carbon dioxide into the killing bin to immerse the packaging material in the liquid carbon dioxide;
the temperature in the sterilizing bin is controlled to be 6-12 ℃, and the temperature of the liquid carbon dioxide is controlled to be 6-12 ℃.
2. The method according to claim 1, wherein the pressure of the liquid carbon dioxide is 5 to 10 MPa.
3. The method of claim 1, wherein the immersion time is 10 to 20 minutes.
4. The method according to claim 1, wherein the concentration of the ethanol solution is 1 to 5 vol%.
5. The method of claim 1, wherein the coronavirus is human seasonal coronavirus hCoV-229E.
6. The method of claim 1, wherein the overwrap material is polyethylene.
7. A method of killing human seasonal coronavirus hCoV-229E on the surface of a polyethylene overwrap material, comprising:
coating 1 volume percent of ethanol solution on the surface of a polyethylene outer packaging material packaged with a cold chain product;
and (3) placing the outer packaging material treated in the previous step into a closed sterilizing bin, controlling the temperature in the sterilizing bin to be 10 ℃ and keeping the sterilizing bin in a vacuum state, and filling liquid carbon dioxide with the pressure of 10 ℃ and 6.3MPa into the sterilizing bin to immerse the outer packaging material into the liquid carbon dioxide for 15 minutes.
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| CN202111636656.9A CN113995870B (en) | 2021-12-30 | 2021-12-30 | Method for killing coronavirus on surface of outer packaging material packaged with cold chain product |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200520799A (en) * | 2003-12-31 | 2005-07-01 | Metal Ind Res & Dev Ct | Method for cleaning and sterilizing medical devices using dense phase fluid |
| CN1788795A (en) * | 2005-12-14 | 2006-06-21 | 浙江大学 | Carbon dioxide pressurized sterilization method under normal temperature |
| US20060292031A1 (en) * | 2004-07-12 | 2006-12-28 | Yung-Ho Chiu | Method of inactivating viruses associated with biomaterial |
| US20090110596A1 (en) * | 2003-06-23 | 2009-04-30 | Timothy Wayne Christensen | Sterilization methods and apparatus which employ additive-containing supercritical carbon dioxide sterilant |
| CN112587697A (en) * | 2020-12-15 | 2021-04-02 | 马东骏 | Device and method for killing new coronavirus in cold chain article |
| CN113424864A (en) * | 2021-08-26 | 2021-09-24 | 中国农业大学 | Method for killing microorganisms on surface of cold-chain food by using low-temperature carbon dioxide |
-
2021
- 2021-12-30 CN CN202111636656.9A patent/CN113995870B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090110596A1 (en) * | 2003-06-23 | 2009-04-30 | Timothy Wayne Christensen | Sterilization methods and apparatus which employ additive-containing supercritical carbon dioxide sterilant |
| TW200520799A (en) * | 2003-12-31 | 2005-07-01 | Metal Ind Res & Dev Ct | Method for cleaning and sterilizing medical devices using dense phase fluid |
| US20060292031A1 (en) * | 2004-07-12 | 2006-12-28 | Yung-Ho Chiu | Method of inactivating viruses associated with biomaterial |
| CN1788795A (en) * | 2005-12-14 | 2006-06-21 | 浙江大学 | Carbon dioxide pressurized sterilization method under normal temperature |
| CN112587697A (en) * | 2020-12-15 | 2021-04-02 | 马东骏 | Device and method for killing new coronavirus in cold chain article |
| CN113424864A (en) * | 2021-08-26 | 2021-09-24 | 中国农业大学 | Method for killing microorganisms on surface of cold-chain food by using low-temperature carbon dioxide |
Non-Patent Citations (1)
| Title |
|---|
| 广西医科大学第一附属医院编: "《新型冠状病毒肺炎临床诊疗与防控管理》", 31 August 2020, 南宁：广西科学技术出版社 * |
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