CN1788697A - Termite olfaction reaction checking device - Google Patents
Termite olfaction reaction checking device Download PDFInfo
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- CN1788697A CN1788697A CN 200510062037 CN200510062037A CN1788697A CN 1788697 A CN1788697 A CN 1788697A CN 200510062037 CN200510062037 CN 200510062037 CN 200510062037 A CN200510062037 A CN 200510062037A CN 1788697 A CN1788697 A CN 1788697A
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- box body
- termite
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Abstract
The termite olfaction reaction detecting device includes one box with upper opening, one upper cover to seal the box in the upper opening and with ventilation hole, at least two connection pipes communicated with the cavity of the box and set symmetrically in the bottom of the box, and sealed containers connected separately via hoses to the connection pipes. All the box, the connection pipes and the sealed containers are made of transparent material. The termite olfaction reaction detecting device of the present invention has high detection sensitivity, and may be used in detecting the olfaction reaction of termite and other insects.
Description
Technical field
The present invention relates to a kind of termite olfaction reaction checking device.
Background technology
Coptotermes formosanus Shtrari. is class existence and the extremely strong social insect of adaptive capacity.They are food with the fibrous material usually, and its harm relates to aspects such as building construction, means of transportation, telecommunication equipment, archives and books, textile, weapons and ammunitions, agriculture and forestry plant.Annual about 20~2,500,000,000 yuan of the economic loss that termite damage causes China.As for the breaking-up that Coptotermes formosanus Shtrari. is caused cultural relics and historic sites, dam reservoirs etc., its consequence then is difficult to weigh with economic data.
Cause harm for various Coptotermes formosanus Shtrari., at present both at home and abroad based on chemical prevention.But the chemical agent that seldom control termites of aiming at is arranged in the world and develop.In fact the development process of termite preventing and controlling agent comprises a large amount of work that existing pesticide species is screened.For example, in the preventative improvement of Coptotermes formosanus Shtrari., need the screening medicament strong, to improve the shield effectiveness of soil chemistry barrier to the Coptotermes formosanus Shtrari. anti-repelling properties.And for example, in termite-killing trapping control, need the suitable decoy of screening and add in the middle of the poison bait, in natural environment, find the time that poison bait is used to shorten Coptotermes formosanus Shtrari., thereby improve whole trapping effect with debita spissitudo.Measuring the influence of reagent agent to the Coptotermes formosanus Shtrari. sensory system quickly and accurately, is the important prerequisite of screening operation success or not.
At present, is no lack of the instrument that the physiological reaction to the insecticide sensory system detects at scientific research field.But those detecting instrument complex structures, involve great expense, and complex operation based on bioelectrical activity; Not only basic unit's control unit makes inquiries for no reason at all, and its testing result also may not have direct directive significance to the medicament screening.
Summary of the invention
At the deficiencies in the prior art part, the invention provides a kind of simple in structure and can accurately detect the checkout gear of Coptotermes formosanus Shtrari. to the food olfaction reaction.
The present invention is for reaching above purpose, be to realize: a kind of termite olfaction reaction checking device is provided by such technical scheme, the box body that comprises upper end open, place at the upper end open place of box body can be with the loam cake of this box body sealing, on cover and be provided with air-vent, the bottom of being close to box body is provided with at least 2 connection tubes that communicate with the inner chamber of box body symmetrically, and the other end of every connection tube is by a hose connection hermetic container; Box body, connection tube, hermetic container are all made by light transmissive material.
A kind of improvement as termite olfaction reaction checking device of the present invention: the periphery at box body is provided with bracing frame, and connection tube is fixed on the bracing frame.
Further improvement as termite olfaction reaction checking device of the present invention: hermetic container is by the transparent bottle and be positioned at this sealing-plug of bleeding bottle bottleneck and form of bleeding; One end of flexible pipe links to each other with connection tube, and bleed therewith the first half of bottle of the other end links to each other.
Further improvement as termite olfaction reaction checking device of the present invention: the bottom at box body and bracing frame is provided with pedestal.
Termite olfaction reaction checking device of the present invention, simple in structure, easy to operate; Its test reaction is highly sensitive, olfactory sensation and taste stimulation can be made a distinction; Therefore use termite olfaction reaction checking device of the present invention, for judging that food is to tested worm---Coptotermes formosanus Shtrari. lure character helpful.Termite olfaction reaction checking device of the present invention also can be common to and detect the olfaction reaction of other walking insecticide to food.
Description of drawings
Fig. 1 is the perspective view of termite olfaction reaction checking device of the present invention;
Fig. 2 is the schematic top plan view after Fig. 1 removes loam cake 1;
Fig. 3 is the actual user mode sketch map of Fig. 2.
The specific embodiment
With reference to above-mentioned accompanying drawing, the specific embodiment of the present invention is elaborated.Fig. 1, Fig. 2 be in conjunction with having provided a kind of termite olfaction reaction checking device, comprises that one is made up of inside and outside two circles and the container of upper end open, and the inner chamber of inner ring and the inner chamber of outer ring are not connected; Inner ring and outer ring are concentric square, and perhaps inner ring and outer ring are the circle of concentric.When inner ring and outer ring are concentric when square, each edge lengths of inner ring is 7~15cm, and each edge lengths of outer ring is 20~30cm.When inner ring and outer ring were concentric circular, inner ring diameter was 5~15cm, and race diameter is 20~40cm.This inner ring promptly becomes box body 3, this outer ring for bracing frame 4.Placing at the upper end open place of box body 3 can be with the loam cake 1 of box body 3 sealings, and loam cake 1 is provided with air-vent 2, the intracavity inter-connection of this air-vent 2 and box body 3; Air-vent 2 can be over against the positive center of box body 3.This box body 3, bracing frame 4, loam cake 1 are made by clear glass.The height of box body 3 is advisable with 3~5cm.Be provided with 2 hermetic containers altogether, the bottle 6 of bleeding that each hermetic container is made by clear glass is formed with the sealing-plug 5 that is positioned at these bottle 6 bottlenecks of bleeding, and sealing-plug 5 plays the effect that seals the bottle 6 of bleeding; It is 250~500ml bottle of bleeding that the bottle 6 of bleeding can be selected capacity for use, and sealing-plug 5 can be made by Cotton Gossypii or silica gel plug.The bottom of being close to box body 3 is provided with the connection tube 9 that 2 clear glasses are made symmetrically with radial, and the radiation initial point is positioned at the positive center of box body 3.The inwall of one end of every connection tube 9 and box body 3 (being inner ring) flushes; The other end of connection tube 9 continues the 1~1.5cm that stretches out after passing bracing frame 4 (being the outer ring), links to each other by flexible pipe 8 the first half with the bottle 6 of bleeding then, and the bracing frame 4 of this moment plays the effect of fixed support connection tube 9.The external diameter of this connection tube 9 is 0.5~0.7cm, and internal diameter should guarantee that Coptotermes formosanus Shtrari. can pass through smoothly; Equally, the internal diameter of flexible pipe 8 must guarantee that also Coptotermes formosanus Shtrari. can pass through smoothly.The pedestal 10 that in the bottom of box body 3 and bracing frame 4 height to be set be 8~10cm.
As shown in Figure 3, the work process that termite olfaction reaction checking device of the present invention is actual when using is as follows:
Earlier Coptotermes formosanus Shtrari. 7 is put into the inner chamber of box body 3, cover loam cake 1 then, prevent that Coptotermes formosanus Shtrari. 7 from fleeing from.Different two kinds of determinands 11,12 (as food or poison bait etc.) are dropped into two bottles 6 of bleeding respectively, and bleeding with 5 pairs of sealing-plugs then bottles 6 seals.Whole detection device is placed in the homothermic incubator of lucifuge.The abnormal smells from the patient of determinand 11,12 all enters the inner chamber of box body 3 by volatilizing naturally along the direction of " bottle 6---flexible pipe 8---connection tube 9 of bleeding ".The Coptotermes formosanus Shtrari. 7 that is arranged in the inner chamber of box body 3 can smell the different abnormal smells from the patient that determinand 11,12 sends, and then according to the attraction of different abnormal smells from the patients to it, creeps to the bottle 6 of bleeding that sends respective smell; The distance of creeping is " inner chamber of box body 3---connection tube a 9---flexible pipe 8 ", falls into the bottle 6 of bleeding accordingly then.Because flexible pipe 8 links to each other with the first half of the bottle 6 of bleeding, the Coptotermes formosanus Shtrari. 7 that therefore falls into the bottle 6 of bleeding can't return the inner chamber of climbing to box body 3 by former distance.After placement after a while, the observer can judge the relative captivation of 11,12 pairs of Coptotermes formosanus Shtrari. 7 of determinand by the quantity of checking Coptotermes formosanus Shtrari. 7 in the different bottles 6 of bleeding.Because Coptotermes formosanus Shtrari. 7 enters all not contacted determinand 11 and determinand 12 before the bottle 6 of bleeding, so determinand 11 and determinand 12 belong to the olfactory sensation category fully for the what is called " captivation " of Coptotermes formosanus Shtrari. 7.
Further specify the effect of this checkout gear below in conjunction with three experiments.Experiment condition is as follows: employed Coptotermes formosanus Shtrari. 7 is eastern subterranean termite Reticulitermes flaviceps in the following experiment, the each test with 10 Coptotermes formosanus Shtrari. 7.Change other 10 behind the EOT.To determinand 11 and 12, test repeats 10 times for each.For the normal Coptotermes formosanus Shtrari. 7 of physiological status, under the ambient temperature about 27 ℃, test once lasts 30 minutes to 1 hour.Surpass 1 hour and go to observe, can not exert an influence result of the test.
In example 1, the bottle 6 of bleeding determinand 11-cane powder is housed, in another bottle 6 of bleeding determinand 12-starch is housed.Observe the number of these two Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 1.(annotate: numeral is the number of the Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistical result marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05).
Table 1 Coptotermes formosanus Shtrari. is to the taxis of cane powder and starch
Cane powder | Starch | |
The | 9 | 1 |
The 2nd Zu the 3rd Zu the 4th Zu the 5th Zu the 6th Zu the 7th Zu the 8th Zu the 9th Zu the 10th Zu average ± | 10 10 9 9 10 9 10 10 10 9.60±0.52 a | 0 0 1 1 0 1 0 0 0 0.40±0.52 b |
In example 2, the bottle 6 of bleeding determinand 11-fermentation cane powder is housed, in another bottle 6 of bleeding the common cane powder of determinand 12-is housed.Observe the number of these two Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 2.(annotate: numeral is the number of the Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistical result marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05)
Table 2 Coptotermes formosanus Shtrari. is to the taxis of fermentation cane powder and common cane powder
The fermentation cane powder | Common cane powder | |
The 1st Zu the 2nd Zu the 3rd Zu the 4th Zu the 5th Zu the 6th Zu the 7th Zu the 8th Zu the 9th Zu the 10th Zu | 9 10 10 10 10 10 9 10 10 10 | 1 0 0 0 0 0 1 0 0 0 |
On average ± SD | 9.80±0.42 a | 0.20±0.42 b |
In example 3, the bottle 6 of bleeding determinand 11-fermentation cane powder is housed, determinand 12-fermentation cane powder+insecticidal active ingredient (40mg/kg) is housed in another bottle 6 of bleeding.Observe the number of these two Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 3.(annotate: numeral is the number of the Coptotermes formosanus Shtrari. 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistical result marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05)
Table 3 Coptotermes formosanus Shtrari. is to the taxis of poison bait
The fermentation cane powder | Fermentation cane powder+insecticidal active ingredient (40mg/kg) | |
The 1st Zu the 2nd Zu the 3rd Zu the 4th Zu the 5th Zu the 6th Zu the 7th Zu the 8th Zu the 9th Zu the 10th Zu average ± | 6 6 6 7 6 5 4 4 5 4 5.30±1.06 a | 4 4 4 3 4 5 6 6 5 6 4.70±1.06 a |
Learn from above-mentioned experiment: cane powder has obviously strong olfactory sensation to attract than starch to Coptotermes formosanus Shtrari. 7; The fermentation cane powder has obviously strong olfactory sensation to attract than common unleavened cane powder to Coptotermes formosanus Shtrari. 7; Add the insecticidal active ingredient of 40mg/kg in the fermentation cane powder made poison bait, its olfactory sensation captivation to Coptotermes formosanus Shtrari. 7 does not have significant difference with fermentation cane powder itself.
More than experimental results show that: termite olfaction reaction checking device of the present invention, test reaction is highly sensitive, can be used to detect the olfaction reaction of insecticides such as Coptotermes formosanus Shtrari. to different determinands.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged, for example change the quantity of bleed bottle 6 and corresponding connection tube 9.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (4)
1, a kind of termite olfaction reaction checking device, it is characterized in that: the box body (3) that comprises upper end open, place at the upper end open place of box body (3) can be with the loam cake (1) of described box body (3) sealing, described loam cake (1) is provided with air-vent (2), the bottom of being close to box body (3) is provided with at least 2 connection tubes (9) that communicate with the inner chamber of box body (3) symmetrically, and the other end of every connection tube (9) connects a hermetic container by flexible pipe (8); Described box body (3), connection tube (9), hermetic container are all made by light transmissive material.
2, termite olfaction reaction checking device according to claim 1 is characterized in that: in the periphery of described box body (3) bracing frame (4) is set, described connection tube (9) is fixed on the bracing frame (4).
3, termite olfaction reaction checking device according to claim 2 is characterized in that: described hermetic container is made up of transparent bleeding bottle (6) and be positioned at described sealing-plug (5) of bleeding bottle (6) bottleneck; One end of described flexible pipe (8) links to each other with connection tube (9), and the other end links to each other with the first half of the bottle (6) of bleeding.
4, termite olfaction reaction checking device according to claim 3 is characterized in that: the bottom at described box body (3) and bracing frame (4) is provided with pedestal (10).
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CNB2005100620378A CN100502803C (en) | 2005-12-16 | 2005-12-16 | Termite olfaction reaction checking device |
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CNB2005100620378A CN100502803C (en) | 2005-12-16 | 2005-12-16 | Termite olfaction reaction checking device |
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CN100502803C CN100502803C (en) | 2009-06-24 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103430909A (en) * | 2013-08-01 | 2013-12-11 | 福建农林大学 | Smell selector for insects |
CN106035120A (en) * | 2016-07-26 | 2016-10-26 | 井冈山大学 | Enclosed two-way selection olfactometer |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103430909A (en) * | 2013-08-01 | 2013-12-11 | 福建农林大学 | Smell selector for insects |
CN106035120A (en) * | 2016-07-26 | 2016-10-26 | 井冈山大学 | Enclosed two-way selection olfactometer |
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