CN1775030A - Efficient water soluble root-inducing powcler for taxus media - Google Patents
Efficient water soluble root-inducing powcler for taxus media Download PDFInfo
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- CN1775030A CN1775030A CN 200510057421 CN200510057421A CN1775030A CN 1775030 A CN1775030 A CN 1775030A CN 200510057421 CN200510057421 CN 200510057421 CN 200510057421 A CN200510057421 A CN 200510057421A CN 1775030 A CN1775030 A CN 1775030A
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Abstract
The present invention relates to a high-effective water soluble rooting powder for Mandiya taxus, belonging to a fast propagation technique for forest tree seed seedling. It uses alpha-naphthylacetic acid and sodium salt or potassium salt powder of indolylbuteric acid as main component, mixes them according to the ratio of 1:0.6-1.5 to obtain the invented rooting powder. In said powder material a proper quantity of rare earth microfertilizer also can be added. Besides, said invention also provides the application method and concrete steps of said rooting powder.
Description
Technical field
The invention belongs to the quick propagating technology of forest seedling, be specifically related to be used for the efficient water soluble root-inducing powder of Taxus x media cottage propagation.
Background technology
Extract the bark from wani et al. from yewtree (Taxus brevifolia Nutt.) and isolate taxol, and find that it has had since the antitumaous effect, the scientific research personnel finds its unique anticancer mechanism again successively and can treat multiple cancer.
Taxol (Taxol) is isolated a kind of diterpene-kind compound in Pacific Ocean Chinese yew (Taxus brevifolia) bark in 1971, it is natural products with unique anticancer mechanism, can induce and promote the tubulin combination, thereby the propagation that stops cancer cell, there are cytotoxicity in oophoroma, breast cancer, cervical carcinoma, lung cancer, CNS cancer, melanoma, liver cancer and leukaemia system etc. to the people, are the cancer therapy drugs of hottest point behind adriamycin and Platinol cisplatin.FDA (FDA) approval taxols in 1992 are used for the treatment of oophoroma and breast cancer.Global taxol total sales volume reached 2,000,000,000 dollars in 2003, and at the speed increase with every year 20%, taxol has become the antineoplastic of the sales volume maximum on the international medical market.
At present, the raw material of producing taxol is mainly derived from bark of Ramulus et folium taxi cuspidatae, and content is about 0.01～0.02%, contains taxol in the blade hardly.Therefore, people begin in large quantities to extract taxol to satisfy the demand of medical market from the bark of Chinese yew.Because Chinese yew worldwide belongs to rare plant, and the age of tree will reach and just have exploitation more than 30 years and be worth, so must seriously destroy natural resources by extract taxol from bark of Ramulus et folium taxi cuspidatae, jeopardizes the ecological balance.Sustainable development of resource has become the biggest problem of taxol raw material at present.1993 is the natural crossing kind of taxus chinensis in northeast and european yew at pacific rim discovery Taxus x media (Taxus media), growth rapidly, with luxuriant foliage and spreading branches in leafy profusion, not only bark contains taxol, and in the leaf content of taxol up to 0.017%～0.051%, the plant of cultivating after 3～5 years is that desirable branches and leaves extract taxol, is the best alternate resources of present bark of Ramulus et folium taxi cuspidatae, and its introducing and planting and a large amount of breeding are had remarkable economical, ecology and social benefit.Improving the cuttage root-taking rate is the key technology of breeding Taxus x media fast, and the domestic report that has only a routine Taxus x media root-inducing powder is not only announced prescription at present, and water insoluble.(Xie Zhiyuan, side's departure, Zhong Jing, the introducing and planting of Taxus x media and the research of fast biostimulants, Chinese herbal medicine 1999 (30) 2:143)
Summary of the invention
The purpose of this invention is to provide a kind of efficient water soluble root-inducing powcler for taxus media, utilize the sodium salt of auxins conditioning agent in the plant growth regulator or the water soluble characteristic of sylvite, the prescription that the promotion Taxus x media of screenings such as orthogonal experiment is taken root is processed into the efficient water soluble root-inducing powder, thereby has increased substantially Taxus x media cuttage root-taking rate and survival rate.
Technical scheme of the present invention is as follows:
It is main component that the present invention selects sodium salt (or sylvite) powder of α-Nai Yisuan and indolebutyric acid for use, both are with 1: the ratio of 0.6-1.5 is mixed into powder, with powder and water dilution proportion, soak Taxus x media cuttage branch during use, soak plugged ear time 2-5h with 600-700mg: 1L.
Wherein, the preparation of described α-Nai Yisuan sodium salt powder is to dissolve in α-Nai Yisuan 9.3g by every 50ml deionized water, and the ratio of sodium hydroxide 1.5-2.0g is dissolved and is placed on 60-80 ℃ of oven dry down, grinds to form powdery at last and obtains.Indolebutyric acid sodium salt powder preparation is to dissolve in indolebutyric acid 10.2g by every 50ml deionized water, and the ratio of sodium hydroxide 1.5-2.0g is dissolved and is placed on 60-80 ℃ of oven dry down, grinds to form powdery at last and obtains.Replacing sodium hydroxide with the potassium hydroxide equivalent when sylvite powder of α-Nai Yisuan and indolebutyric acid is prepared gets final product.
The present invention has utilized the principle of plant auxins hestening rooting, and NAA promotes thick root to form, and IBA promotes radicula to form, and both dispose by a certain percentage, and rooting efficiency is fabulous.
For further hestening rooting, also can add the RE microbial fertilizer of the about 1/6-1/8 of powder quality in the powder.RE microbial fertilizer has the volume increase of promotion, improves the report of quality.Do not see the report that adds the RE microbial fertilizer hestening rooting in the root-inducing powder as yet.RE microbial fertilizer is handled plugged ear, obviously promotes the stem end to expand, nutrient accumulation, thereby hestening rooting.
The invention has the advantages that 1, root-inducing powder is soluble in water, uses more conveniently, and commercially available root-inducing powder needs earlier with the alcohol dissolving, again dilute with water; 2, the prescription through orthogonal experiment screening reaches 97.1% rooting rate, and higher report is not seen in contrast only 27.5%; 3, after RE microbial fertilizer is handled, expand by promoting metabolism and callus, and then hestening rooting.Have not yet to see the report that adds the RE microbial fertilizer hestening rooting in the root-inducing powder.
Embodiment
Embodiment 1:
A. weighing sodium hydroxide 2.00g, α-Nai Yisuan (NAA) 9.31g is dissolved in the 50ml deionized water, dissolves to be placed on 80 ℃ of oven dry down, grinds to form powdery.
B. weighing sodium hydroxide 2.00g, indolebutyric acid (IBA) 10.16g is dissolved in the 50ml deionized water, dissolves to be placed on 80 ℃ of oven dry down, grinds to form powdery.
Every bag of efficient water soluble root-inducing powcler for taxus media system takes by weighing A 335.4mg, B 332.5mg, mixing, packing.Outward appearance: white powder, water soluble.
Using method: every bag of root-inducing powder is directly dissolved with the 1000ml running water, with Taxus x media cuttage branch 10-15cm, two ends are whittled into the inclined-plane with sharp pocket knife, and the blade of lower end 1/3 is removed, and soak 3 hours in above-mentioned solution, insert in the pearlite interstitial substance then, keep soil humidity, fine shading screen with 90% shades, and takes root successively after 40 days, rooting rate reached 95.24% (contrast is 1.67%) in 70 days, and rooting rate reached 97.1% (contrast is 27.5%) in 90 days.Last survival rate reaches more than 98%.Utilize above-mentioned sound root powder, bred 80,000 strains in 1 year.
Embodiment 2:A. takes by weighing potassium hydroxide 9.75g, takes by weighing α-Nai Yisuan (NAA) 46.5g, is dissolved in the 250ml deionized water, dissolves to be placed on 70 ℃ of oven dry down, grinds to form powdery.
B. take by weighing potassium hydroxide 9.75g, indolebutyric acid (IBA) 51g is dissolved in the 250ml deionized water, dissolves to be placed on 70 ℃ of oven dry down, grinds to form powdery.
Every bag of efficient water soluble root-inducing powcler for taxus media system takes by weighing each 0.33g of A, B, RE microbial fertilizer 0.1g, and mixing, packing, every bag is dissolved in 1 liter of running water.Outward appearance: white powder is carried rose pink crystal, water soluble secretly.
Result of use is as follows:
| Handle number | The root-inducing powder prescription | The stem blackening | Stem is green or expand | Root length is less than 3mm | Root length is less than 2cm | Root is grown up in 2cm, and quantity is many | Rooting rate (%) |
| 1 | Root-inducing powder | 1 | 3 | 28 | 22 | 30 | 95.24 |
| 2 | RE microbial fertilizer 100mg/L | 0 | 118 | 2 | 0 | 0 | 0.02 |
| 3 | Root-inducing powder+RE microbial fertilizer | 1 | 24 | 30 | 38 | 37 | 80.77 |
Annotate: plugged ear soak time 3h, the 2004.2.28. cuttage is handled, the 5.7. statistics
Embodiment 3 orthogonal experiment 2003.3.10. cuttages are handled, the 4.26. statistics
| L 9(3 4) | 1 | 2 | 3 | 4 |
| Factor level | A(NAAmg/L) | B(IBAmg/L) | C(h) | D |
| 1 | 100 | 100 | 1 | |
| 2 | 200 | 200 | 3 | |
| 3 | 300 | 300 | 5 |
Perlite cuttage orthogonal experiments and variance analysis
| A | B | C | D | Initial data X i(rank/strain number) | ∑X i | Xi 2 | (∑X i) 2 |
| 1 | 1 | 1 | 1 | 1 | 1/1，2/3，3/12，4/4，5/0 | -2 | 44 | 4 |
| 2 | 1 | 2 | 2 | 2 | 1/0，2/5，3/6，4/9，5/1 | 12 | 72 | 144 |
| 3 | 1 | 3 | 3 | 3 | 1/0，2/0，，3/6，4/11，5/3 | 34 | 92 | 1156 |
| 4 | 2 | 1 | 2 | 3 | 1/0，2/2，3/10，4/6，5/2 | 16 | 64 | 256 |
| 5 | 2 | 2 | 3 | 1 | 1/0，2/6，3/4，4/6，5/5 | 12 | 88 | 144 |
| 6 | 2 | 3 | 1 | 2 | 1/0，2/5，3/4，4/6，5/5 | 26 | 124 | 676 |
| 7 | 3 | 1 | 3 | 2 | 1/0，2/4，3/6，4/4，5/6 | 24 | 128 | 576 |
| 8 | 3 | 2 | 1 | 3 | 1/0，2/4，3/5，4/9，5/2 | 18 | 84 | 324 |
| 9 | 3 | 3 | 2 | 1 | 1/0，2/2，3/2，4/8，5/8 | 44 | 168 | 1936 |
| K 1 | 44 | 38 | 42 | 184 | 864 | 5216 | ||
| K 2 | 54 | 42 | 72 | |||||
| K 3 | 86 | 104 | 70 | |||||
| K 1 2 | 1936 | 1444 | 1764 | |||||
| K 2 2 | 2916 | 1764 | 5184 | |||||
| K 3 2 | 7396 | 10816 | 4900 |
Cuttage root-taking effect grading standard:
1 grade: stem blackening, no callus strain number * (4)
2 grades: the stem green, no callus, or stem expands stem black strain number * (2)
3 grades: stem expands, and callus is arranged, the strain number of not taking root as yet * (0)
4 grades: callus is arranged, have root to take place, root is less than 1mm strain number * (2)
5 grades: callus is arranged, have root to take place, root is greater than 1mm, many strains of quantity number * (4)
Variance analysis table
| Soruces of variation | Sum of square of deviations SS | Degree of freedom | Mean deviation sum of squares MS | The F value |
| A B C merges Se | 16.0 45.6 9.4 604.9 | 2 2 2 173 | 8.0 22.8 4.7 5.2 | 1.54 4.38* 0.90 |
F tables look-up
0.01 (2,173=4.73 F
0.05 (2,173=3.05
Conclusion: IBA appreciable impact Taxus x media cuttage root-taking.Best of breed is NAA300mg/L, IBA300mg/L, plugged ear soak time 3h.
Embodiment 4:
| Handle | Sample number | Sampling | 1 grade | 2 grades | 3 grades | 4 grades | 5 grades | Take root the date the earliest | 5.2. observation rooting rate | 6.12. observation rooting rate |
| Contrast | 700 | 70 | 11 | 58 | 1 | 0 | 0 | 0 | 27.5 | |
| NAA300mg/L +IBA300mg/L | 700 | 70 | 0 | 25 | 21 | 17 | 7 | 4.22 | 64.3 | 97.1 |
| The oven dry preparation | ||||||||||
| NAA300mg/L+IBA300mg/L+Vc300mg/L is dried preparation | 700 | 70 | 11 | 27 | 14 | 9 | 9 | 45.9 | 90.0 | |
| NAA300mg/L+IBA300mg/L microwave drying preparation | 700 | 70 | 14 | 31 | 11 | 9 | 5 | 35.7 | 71.4 |
Annotate: plugged ear soak time 3h.2004.3.12. cuttage, 5.2. spot-check rooting rate, 6.12. selective examination rooting rate and hierarchical statistics, and kind Hicksii annotinous branch shades, under the natural temperature, pearlite interstitial substance:
Grading standard: 1 grade: no callus, stem brown stain
2 grades: callus is arranged, and stem is brown stain not
3 grades: gone out root 1-2 root, root length is less than 0.3mm
4 grades: take root better, 2-5 bar root is arranged, root length is less than 1cm,
5 grades: root is many and grow, and root is grown up in 1cm
The result shows that the sodium salt effect of utilizing oven dry to prepare NAA and IBA is better than microwave drying; Add vitamin C in the root-inducing powder and do not have positive effect.
Claims (3)
1, efficient water soluble root-inducing powcler for taxus media, it is characterized in that it includes the sodium salt or the sylvite powder of α-Nai Yisuan and indolebutyric acid, both are with 1: the ratio of 0.6-1.5 is mixed into powder, during use with powder and water dilution proportion with 600-700mg: 1L, soak Taxus x media cuttage branch, soak plugged ear time 2-5h.
2, efficient water soluble root-inducing powcler for taxus media according to claim 1, described α-Nai Yisuan sodium salt powder preparation is to dissolve in α-Nai Yisuan 9.3g by every 50ml deionized water when it is characterized in that described preparation α-Nai Yisuan sodium salt powder, the ratio of sodium hydroxide 1.5-2.0g, dissolve and be placed on 60-80 ℃ of down oven dry, grind to form powdery at last and obtain; During preparation indolebutyric acid sodium salt powder is to dissolve in indolebutyric acid 10.2g by every 50ml deionized water, and the ratio of sodium hydroxide 1.5-2.0g is dissolved and is placed on 60-80 ℃ of oven dry down, grinds to form powdery at last and obtains; Replacing sodium hydroxide with the potassium hydroxide equivalent when sylvite powder of α-Nai Yisuan and indolebutyric acid is prepared gets final product.
3, efficient water soluble root-inducing powcler for taxus media according to claim 1 is characterized in that also can adding in the powder RE microbial fertilizer of the about 1/6-1/8 of powder quality.
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Cited By (7)
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| CN102037967A (en) * | 2010-09-19 | 2011-05-04 | 浙江农林大学 | Method for improving Dendrobium officinale hybridization seed setting rate and special treating agent thereof |
| CN102210305A (en) * | 2011-04-08 | 2011-10-12 | 天津绿动植物营养技术开发有限公司 | Preparation for promoting plant to root |
| CN104585249A (en) * | 2015-02-06 | 2015-05-06 | 江西万茂科技有限公司 | Solution capable of promoting rooting of taxus media |
| CN104604940A (en) * | 2015-02-04 | 2015-05-13 | 安徽泓森高科生态农林开发有限公司 | Hong sen locust rooting agent |
| CN104956880A (en) * | 2015-06-15 | 2015-10-07 | 孝感市大自在生态农业发展有限公司 | Rapid propagation method for taxus media |
| CN105123379A (en) * | 2015-07-20 | 2015-12-09 | 重庆紫逸农业发展有限公司 | Method for rapidly breeding Taxus media |
| CN107897182A (en) * | 2017-11-17 | 2018-04-13 | 中山大学 | A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102037967A (en) * | 2010-09-19 | 2011-05-04 | 浙江农林大学 | Method for improving Dendrobium officinale hybridization seed setting rate and special treating agent thereof |
| CN102037967B (en) * | 2010-09-19 | 2013-06-12 | 浙江农林大学 | Method for improving Dendrobium officinale hybridization seed setting rate and special treating agent thereof |
| CN102210305A (en) * | 2011-04-08 | 2011-10-12 | 天津绿动植物营养技术开发有限公司 | Preparation for promoting plant to root |
| CN104604940A (en) * | 2015-02-04 | 2015-05-13 | 安徽泓森高科生态农林开发有限公司 | Hong sen locust rooting agent |
| CN104604940B (en) * | 2015-02-04 | 2017-12-12 | 安徽泓森高科林业股份有限公司 | A kind of deep gloomy Chinese scholartree root-growing agent |
| CN104585249A (en) * | 2015-02-06 | 2015-05-06 | 江西万茂科技有限公司 | Solution capable of promoting rooting of taxus media |
| CN104956880A (en) * | 2015-06-15 | 2015-10-07 | 孝感市大自在生态农业发展有限公司 | Rapid propagation method for taxus media |
| CN105123379A (en) * | 2015-07-20 | 2015-12-09 | 重庆紫逸农业发展有限公司 | Method for rapidly breeding Taxus media |
| CN107897182A (en) * | 2017-11-17 | 2018-04-13 | 中山大学 | A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout |
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