CN107897182A - A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout - Google Patents
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout Download PDFInfo
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- CN107897182A CN107897182A CN201711147720.0A CN201711147720A CN107897182A CN 107897182 A CN107897182 A CN 107897182A CN 201711147720 A CN201711147720 A CN 201711147720A CN 107897182 A CN107897182 A CN 107897182A
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- branch
- cuttage
- taking root
- radix baccaureae
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 238000005520 cutting process Methods 0.000 claims abstract description 43
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- -1 methyl α-naphthyl acetates Chemical class 0.000 claims abstract description 14
- 239000005720 sucrose Substances 0.000 claims abstract description 14
- LBLSRDDHGILUJH-UHFFFAOYSA-N 4-(1h-indol-2-yl)butanoic acid Chemical class C1=CC=C2NC(CCCC(=O)O)=CC2=C1 LBLSRDDHGILUJH-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004576 sand Substances 0.000 claims description 38
- 230000008774 maternal effect Effects 0.000 claims description 15
- 239000003755 preservative agent Substances 0.000 claims description 15
- 230000002335 preservative effect Effects 0.000 claims description 15
- 238000002791 soaking Methods 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 8
- 230000037431 insertion Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 230000003442 weekly effect Effects 0.000 claims description 8
- 230000000844 anti-bacterial effect Effects 0.000 claims description 6
- 239000003899 bactericide agent Substances 0.000 claims description 6
- 238000001228 spectrum Methods 0.000 claims description 6
- 230000001737 promoting effect Effects 0.000 claims 1
- 238000012545 processing Methods 0.000 abstract description 20
- 235000013399 edible fruits Nutrition 0.000 abstract description 12
- 241000196324 Embryophyta Species 0.000 description 15
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 13
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 13
- 239000006013 carbendazim Substances 0.000 description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 12
- 206010020649 Hyperkeratosis Diseases 0.000 description 10
- 230000008520 organization Effects 0.000 description 7
- 229910052742 iron Inorganic materials 0.000 description 6
- 239000007921 spray Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 239000002023 wood Substances 0.000 description 5
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 241000221017 Euphorbiaceae Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-M 1-naphthaleneacetate Chemical compound C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-M 0.000 description 1
- GOLXRNDWAUTYKT-UHFFFAOYSA-N 3-(1H-indol-3-yl)propanoic acid Chemical compound C1=CC=C2C(CCC(=O)O)=CNC2=C1 GOLXRNDWAUTYKT-UHFFFAOYSA-N 0.000 description 1
- 235000015079 Baccaurea Nutrition 0.000 description 1
- 241000785762 Baccaurea Species 0.000 description 1
- 235000002289 Baccaurea ramiflora Nutrition 0.000 description 1
- 241000891306 Baccaurea ramiflora Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 244000089795 Clausena lansium Species 0.000 description 1
- 235000008738 Clausena lansium Nutrition 0.000 description 1
- 241000776116 Cyphostemma juttae Species 0.000 description 1
- 244000183278 Nephelium litchi Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000010426 asphalt Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 235000005523 excessive nutrition Nutrition 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009417 vegetative reproduction Effects 0.000 description 1
- 238000013466 vegetative reproduction Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to fruit tree cultivation technical field, specifically disclose a kind of promotion Radix Baccaureae ramiflorae cuttage branch and take root the taking root liquid to sprout, be made of each component of following mass fraction：50~500 ‰ methyl α-naphthyl acetates, 300~900 ‰ indolebutyric acids, 5~10% sucrose, 0.3~09%KMnO4, 0.5~5% ethanol.Liquid energy provided by the present invention of taking root effectively facilitates Radix Baccaureae ramiflorae cuttage branch and takes root, and improves rooting rate, solves the problems, such as to be difficult to take root during Radix Baccaureae ramiflorae cutting propagation.The invention also discloses a kind of promote Radix Baccaureae ramiflorae cuttage branch to take root the method that sprouts, include processing, cuttage and the shading and final-period management of cuttage branch.Using this method the rooting rate of cutting and sprouting can be promoted to take out hair rate, for rooting rate up to more than 75%, the radical amount born is more, and seedling branch is sturdy, sprout is full, shortens nursery stage, and this method is environmentally safe, is suitable for large-scale promotion and application.
Description
Technical field
The present invention relates to fruit tree cultivation technical field, and in particular, to one kind promotion Radix Baccaureae ramiflorae cuttage branch, which is taken root, to sprout
Method.
Background technology
Radix Baccaureae ramiflorae（Baccaurea ramiflora Lour.）, it is also known as white skin, Shan Luo Portugals, dunn wampee leaf or root tree, mountain beans, wooden litchi
Branch, Dalian fruit, yellow fruit tree, tree grape etc., belong to Euphorbiaceae（Euphorbiaceae）Radix Baccaureae ramiflorae category（Baccaurea）Evergreen shrubs
Or arbor, tree are 6~12 meters high.It is distributed mainly on the South Asia such as India, Burma, Thailand, Malaysia, Vietnam and Southeast Asia state at present
Family, China are then distributed mainly on that south China is low, Zhonghai36-1 asphalt, often mixed to be born in other woods.Radix Baccaureae ramiflorae tree body is graceful, and fruit is beautiful
Taste, be integrate view and admire, eat, medicinal multipurpose tree species, a kind of and plant that economic value potentiality to be exploited is high.
Radix Baccaureae ramiflorae is more at home to be distributed in low, intermediate altitude mountain valley, hillside forest land with wild state, and not yet large area is artificial
Cultivation.And in terms of Radix Baccaureae ramiflorae vegetative propagation cuttage cultivation, then, there is correlative study report wood in the problem of generally existing is difficult to take root
The cutting propagation of milk fruit, it uses a biennial or perennial branch, is divided into two groups, respectively with ABT root-inducing powders 1 and methyl α-naphthyl acetate
500th, 1000,1500mg/L processing, first sprouts after processing and produces preferable callus, but does not take root always, still need into
One step research（Hu Jianxiang etc., wild fruit tree -- Radix Baccaureae ramiflorae [J] southern china fruit trees, 2003,32 (4):49-49.）.At present still
Have no the relevant report for having Radix Baccaureae ramiflorae cuttage branch can be promoted to take root long bud method in the prior art.
The content of the invention
The present invention is in order to overcome the above-mentioned deficiency of the prior art, there is provided one kind promotes Radix Baccaureae ramiflorae cuttage branch to take root to sprout
Taking root liquid.
A kind of method to sprout another object of the present invention is to provide promotion Radix Baccaureae ramiflorae cuttage branch to take root.
To achieve these goals, the present invention is achieved by following scheme：
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the taking root liquid to sprout, is made of each component of following mass fraction：50~
500 ‰ methyl α-naphthyl acetates, 300~900 ‰ indolebutyric acids, 5~10% sucrose, 0.3~09% KMnO4, 0.5~5% ethanol.
Preferably, the taking root liquid is made of each component of following mass fraction：100~400 ‰ methyl α-naphthyl acetates, 400~
700 ‰ indolebutyric acids, 6~8% sucrose, 0.6~0.8% KMnO4, 1~5% ethanol.
It is highly preferred that the taking root liquid is made of each component of following mass fraction：300 ‰ methyl α-naphthyl acetates, 600 ‰ indoles fourths
Acid, 7% sucrose, 0.7% KMnO4, 2% ethanol.
The present invention also protects taking root liquid the answering in promotion Radix Baccaureae ramiflorae cuttage branch takes root and sprouts described in any of the above-described
With.
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
S1. 1~3 year raw branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch, the rugosity of branch is 1.0~3.0cm,
Length is 20~25cm；Burnt immediately after cutting and seal and wrapped up with preservative film, removed whole branch after leaf with preservative film
Parcel；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into 15cm, branch base portion is cut to angle shape, then
Branch is immediately placed in any of the above-described taking root liquid and soaks 2h, the soaking depth of branch bottom is 7cm；
S2. by the branch cutting after being soaked in S1 in by the sand bed disinfected, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 5~7cm away from the depth for 20~22cm, insertion；After cuttage cutting is sprayed with wide-spectrum bactericide；Covered above sand bed at 1m
First layer shading net, shading rate are 60%；Second layer shading net is set again above sand bed at 2m, and shading rate is 60%, is carried out double-deck
Shading；First layer shading net is removed after 90d；
S3. watering 2 times daily after cuttage；Weekly branch is sprayed with wide-spectrum bactericide 1 time；Each branch is sprayed every 15 days
100~120mL nutrient solutions；After 50d, 50~100mL taking root liquids are poured to each branch every 10d.
Using the measure for burning branch in S1 of the present invention, be conducive to the preservation of branch and transport for a long time, can either prevent
Branch bacterial infection or germ, so as to influence rooting rate；Milk can be prevented to be lost in again, milk, which is lost in, is excessively easy to cause nutrition
Be lost in it is excessive, so as to influence taking root below.Preferably, the time for burning branch is 3s.
1~3 year raw branch is selected in S1 as cuttage branch, the rugosity of branch is 1.0~3.0cm, length for 20~
25cm, is conducive to the cuttage root-taking of branch, this is because the advanced row vegetative reproduction of branch after cuttage, if branch is too tender too thin, disappears
Excessive nutrition is consumed, then influences to take root, or even be difficult to take root；If branch life more than 3 years, aging is serious, influences to take root, or even difficult
To take root.Preferably, the branch is life in 2 years, and the rugosity of branch is 2.0cm, length 25cm.
It is to induce branch to produce callus that branch, which is immersed in taking root liquid, in S1.Taking root liquid is poured to branch in S4
It is in order to which evoked callus is taken root, it is preferable that the dosage of taking root liquid described in S4 is 100mL.
Sand bed described in S2 disinfect for be 0.4~1.0% with mass fraction KMnO4Solution carries out disinfection, and is exposed to the sun
2d。
It is tropical rain forest typically middle lower floor forest tree since cloudy drought-enduring, the light shade tolerant of Radix Baccaureae ramiflorae happiness, resistance are stronger
Kind, double-deck shading is carried out in S3 and is more advantageous to taking root, prevents that nutrient growth from overrunning nutriment.Used in S2 and S3 after cuttage
Wide-spectrum bactericide sprinkling branch can prevent cuttage seeding from growing germ.
Preferably, the wide-spectrum bactericide is 1000 times of carbendazim.
Mode is preferably carried out as one kind, and the promotion Radix Baccaureae ramiflorae cuttage branch method to sprout of taking root includes following tool
Body step：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose the raw branch conduct in 1~3 year on ripe Radix Baccaureae ramiflorae maternal plant
Cuttage branch, the rugosity of branch is 1.0~3.0cm, and length is 20~25cm；Sealed and used fresh-keeping with baked wheaten cake 3s immediately after cutting
Film tightly wraps up, and is wrapped up whole branch with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then will
Branch length is cut into 15cm, and branch base portion is cut to 15 ° of angle shapes, and then branch is immediately placed in any of the above-described taking root liquid
2h is soaked, the soaking depth of branch bottom is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 0.4~1.0% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 5~7cm away from the depth for 20~22cm, insertion；After cuttage branch is sprayed with 1000 times of carbendazim；Iron wire is curved arch to insert
In sand bed, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；The second layer is set again at 2m above sand bed
Shading net, shading rate are 60%, carry out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly branch is sprayed with 1000 times of carbendazim 1 time；Every 15 days to each
Branch sprays 100~120mL nutrient solutions；After 50d, 50~100mL taking root liquids are poured to each branch every 10d.
Compared with prior art, the invention has the advantages that：
（1）Liquid energy provided by the present invention of taking root effectively facilitates Radix Baccaureae ramiflorae cuttage branch and takes root, and improves rooting rate, solves wood
The problem of being difficult to take root during milk fruit cutting propagation.
（2）Method provided by the present invention, can promote cutting rooting rate and sprouting take out hair rate, rooting rate up to 75% with
On, the radical amount born is more, every Radix Baccaureae ramiflorae cuttage branch average energy produce more than 60 sprouting, the new root of more than 20；
Seedling branch is sturdy, sprout is full, shortens nursery stage, and seedling cost significantly declines；Method provided by the present invention is to environment
It is pollution-free, it is suitable for large-scale promotion and application.
Brief description of the drawings
Fig. 1~4 are the situation of sprouting of taking root after Radix Baccaureae ramiflorae branch cutting 120d in embodiment 6.
Fig. 5~8 are the situation of sprouting of taking root after Radix Baccaureae ramiflorae branch cutting 120d in comparative example 3.
Embodiment
The present invention is made with reference to Figure of description and specific embodiment and further being elaborated, the embodiment
It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy
Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained
And material.
Embodiment 1 is a kind of to promote Radix Baccaureae ramiflorae cuttage branch to take root the taking root liquid to sprout
1st, the taking root liquid of following mass fraction is configured：300 ‰ methyl α-naphthyl acetates, 600 ‰ indolebutyric acids, 7% sucrose, 0.7% KMnO4, 2%
Ethanol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating 50d, the Radix Baccaureae ramiflorae branch after discovery processing
Notch first sprouts and produces preferable callus；Culture produces root after 120 days, and average every Radix Baccaureae ramiflorae cuttage branch can produce
The sprouting of more than 20, more than 5 new roots.
Embodiment 2 is a kind of to promote Radix Baccaureae ramiflorae cuttage branch to take root the taking root liquid to sprout
1st, the taking root liquid of following mass fraction is configured：100 ‰ methyl α-naphthyl acetates, 500 ‰ indolebutyric acids, 6% sucrose, 0.4% KMnO4,
1.5% ethanol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating 50d, the Radix Baccaureae ramiflorae branch after discovery processing
Notch first sprouts and produces preferable callus；Culture produces root after 120 days, and average every Radix Baccaureae ramiflorae cuttage branch can produce
The sprouting of more than 15, more than 3 new roots.
Embodiment 3 is a kind of, and to promote wood be that fruit cuttage branch is taken root the taking root liquid to sprout
1st, the taking root liquid of following mass fraction is configured：450 ‰ methyl α-naphthyl acetates, 700 ‰ indolebutyric acids, 8% sucrose, 0.5% KMnO4, 3%
Ethanol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating 50d, the Radix Baccaureae ramiflorae branch after discovery processing
Notch first sprouts and produces preferable callus；Culture produces root after 120 days, and average every Radix Baccaureae ramiflorae cuttage branch can produce
The sprouting of more than 13, more than 4 new roots.
Embodiment 4 is a kind of, and to promote wood be that fruit cuttage branch is taken root the taking root liquid to sprout
1st, the taking root liquid of following mass fraction is configured：300 ‰ methyl α-naphthyl acetates, 400 ‰ indolebutyric acids, 8% sucrose, 0.8% KMnO4,
4.5% ethanol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating 50d, the Radix Baccaureae ramiflorae branch after discovery processing
Notch first sprouts and produces preferable callus；Culture produces root after 120 days, and average every Radix Baccaureae ramiflorae cuttage branch can produce
The sprouting of more than 14, more than 3 new roots.
Embodiment 5 is a kind of, and to promote wood be that fruit cuttage branch is taken root the taking root liquid to sprout
1st, the taking root liquid of following mass fraction is configured：300 ‰ methyl α-naphthyl acetates, 600 ‰ indolebutyric acids, 7% sucrose, 0.7% KMnO4, 2%
Ethanol.
2nd, 3 years raw branches on ripe Radix Baccaureae ramiflorae maternal plant are chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating 50d, the Radix Baccaureae ramiflorae branch after discovery processing
Notch first sprouts and produces preferable callus；Culture produces root after 120 days, and average every Radix Baccaureae ramiflorae cuttage branch can produce
The sprouting of more than 18, more than 4 new roots.
Comparative example 1
1st, the solution of following mass fraction is configured：40 ‰ methyl α-naphthyl acetates, 200 ‰ indolebutyric acids, 8% sucrose, 0.5% KMnO4, 3% second
Alcohol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating a period of time, finds Radix Baccaureae ramiflorae cuttage branch
Bar can only produce callus, but can not take root.
Comparative example 2
1st, the solution of following mass fraction is configured：600 ‰ methyl α-naphthyl acetates, 950 ‰ indolebutyric acids, 8% sucrose, 0.5% KMnO4, 3% second
Alcohol.
2nd, the biennial branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch；It is immersed in after cuttage branch is sterilized
In the taking root liquid that step 1 configures, conventional organization culture is carried out after soaking 2h, after cultivating a period of time, finds Radix Baccaureae ramiflorae cuttage branch
Bar can only produce callus, but can not take root.
Embodiment 6
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose 2 years raw branches on ripe Radix Baccaureae ramiflorae maternal plant as skewer
Transplant bar, the rugosity of branch is 2.0cm, length 25cm；Sealed with baked wheaten cake 3s after cutting and tightly wrapped up with preservative film immediately,
Whole branch is wrapped up with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into
15cm, branch base portion are cut to angle shape, and then branch is immediately placed in the taking root liquid of embodiment 1 and soaks 2h, branch bottom
Soaking depth is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 0.4% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 5cm away from the depth for 20cm, insertion；After cuttage cutting is sprayed with 1000 times of carbendazim；Iron wire is curved into arch and inserts in sand bed
In, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；Second layer shading net is set again at 2m above sand bed,
Shading rate is 60%, carries out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly cutting 1 time is sprayed with 1000 times of carbendazim；Every 15 days to each
Branch sprays 100mL nutrient solutions；After 50d, 100mL taking root liquids are poured to each branch every 10d.
Cuttage, as shown in figures 1-4, every Radix Baccaureae ramiflorae cuttage branch after 120d are carried out to Radix Baccaureae ramiflorae branch using the above method
Average energy produces the sprouting of more than 60, more than 20 new roots, and rooting rate is more than 75%, average root long 2.7cm, and average root is thick
0.16cm, sprouting take out hair rate 97%, sprout turgor 82%.
Embodiment 7
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose 1 year raw branch on ripe Radix Baccaureae ramiflorae maternal plant as skewer
Transplant bar, the rugosity of branch is 1.0cm, length 20cm；Sealed with baked wheaten cake 3s after cutting and tightly wrapped up with preservative film immediately,
Whole branch is wrapped up with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into
15cm, branch base portion are cut to angle shape, and then branch is immediately placed in the taking root liquid of embodiment 2 and soaks 2h, branch bottom
Soaking depth is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 0.6% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 6cm away from the depth for 21cm, insertion；After cuttage cutting is sprayed with 1000 times of carbendazim；Iron wire is curved into arch and inserts in sand bed
In, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；Second layer shading net is set again at 2m above sand bed,
Shading rate is 60%, carries out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly cutting 1 time is sprayed with 1000 times of carbendazim；Every 15 days to each
Branch sprays 100mL nutrient solutions；After 50d, 50mL taking root liquids are poured to each branch every 10d.
Cuttage carries out Radix Baccaureae ramiflorae branch using the above method, every Radix Baccaureae ramiflorae cuttage branch average energy produces 23 after 120d
Sprouting above, more than 11 new roots, rooting rate 54%.
Embodiment 8
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose 3 years raw branches on ripe Radix Baccaureae ramiflorae maternal plant as skewer
Transplant bar, the rugosity of branch is 3.0cm, length 25cm；Sealed with baked wheaten cake 3s after cutting and tightly wrapped up with preservative film immediately,
Whole branch is wrapped up with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into
15cm, branch base portion are cut to angle shape, and then branch is immediately placed in the taking root liquid of embodiment 3 and soaks 2h, branch bottom
Soaking depth is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 1.0% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 7cm away from the depth for 22cm, insertion；After cuttage cutting is sprayed with 1000 times of carbendazim；Iron wire is curved into arch and inserts in sand bed
In, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；Second layer shading net is set again at 2m above sand bed,
Shading rate is 60%, carries out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly cutting 1 time is sprayed with 1000 times of carbendazim；Every 15 days to each
Branch sprays 100mL nutrient solutions；After 50d, 100mL taking root liquids are poured to each branch every 10d.
Cuttage carries out Radix Baccaureae ramiflorae branch using the above method, every Radix Baccaureae ramiflorae cuttage branch average energy produces 37 after 120d
Sprouting above, more than 13 new roots, rooting rate 62%.
Embodiment 9
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose 2 years raw branches on ripe Radix Baccaureae ramiflorae maternal plant as skewer
Transplant bar, the rugosity of branch is 2.0cm, length 25cm；Sealed with baked wheaten cake 3s after cutting and tightly wrapped up with preservative film immediately,
Whole branch is wrapped up with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into
15cm, branch base portion are cut to angle shape, and then branch is immediately placed in the taking root liquid of embodiment 4 and soaks 2h, branch bottom
Soaking depth is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 0.4% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 5cm away from the depth for 20cm, insertion；After cuttage cutting is sprayed with 1000 times of carbendazim；Iron wire is curved into arch and inserts in sand bed
In, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；Second layer shading net is set again at 2m above sand bed,
Shading rate is 60%, carries out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly cutting 1 time is sprayed with 1000 times of carbendazim；Every 15 days to each
Branch sprays 100mL nutrient solutions；After 50d, 60mL taking root liquids are poured to each branch every 10d.
Cuttage carries out Radix Baccaureae ramiflorae branch using the above method, every Radix Baccaureae ramiflorae cuttage branch average energy produces 45 after 120d
Sprouting above, more than 18 new roots, rooting rate 68%.
Embodiment 10
A kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root the method to sprout, is comprised the following steps：
1st, the processing of cuttage branch：Annual early and middle ten days in May, choose 3 years raw branches on ripe Radix Baccaureae ramiflorae maternal plant as skewer
Transplant bar, the rugosity of branch is 3.0cm, length 25cm；Sealed with baked wheaten cake 3s after cutting and tightly wrapped up with preservative film immediately,
Whole branch is wrapped up with preservative film after removing leaf；The bottom 1cm of branch sealing is cut before cuttage, then branch length is cut into
15cm, branch base portion are cut to angle shape, and then branch is immediately placed in the taking root liquid of embodiment 5 and soaks 2h, branch bottom
Soaking depth is 7cm；
2nd, sand bed processing early period：With the KMnO that mass fraction is 0.4% before cuttage4Solution carries out disinfection, and be exposed to the sun 2d；
3rd, cuttage and shading：The branch cutting after 2h will be soaked in step 1 in sand bed, towards south to 60 ° of oblique cuttings, the row of cuttage
It is 5cm away from the depth for 20cm, insertion；After cuttage cutting is sprayed with 1000 times of carbendazim；Iron wire is curved into arch and inserts in sand bed
In, first layer shading net is being covered above sand bed at 1m, shading rate is 60%；Second layer shading net is set again at 2m above sand bed,
Shading rate is 60%, carries out double-deck shading；First layer shading net is removed after 90d；
4th, final-period management：Watering 2 times daily after cuttage；Weekly cutting 1 time is sprayed with 1000 times of carbendazim；Every 15 days to each
Branch sprays 100mL nutrient solutions；After 50d, 90mL taking root liquids are poured to each branch every 10d.
Cuttage carries out Radix Baccaureae ramiflorae branch using the above method, every Radix Baccaureae ramiflorae cuttage branch average energy produces 46 after 120d
Sprouting above, more than 19 new roots, rooting rate 68%.
Comparative example 3
Cuttage is carried out to Radix Baccaureae ramiflorae branch using the method for embodiment 1, except formula of liquid difference of taking root, remaining all same.Life used
Root liquid is the taking root liquid of comparative example 1, and as shown in figures 5-8, Radix Baccaureae ramiflorae cuttage branch cannot take root after 120d.
Claims (8)
- The taking root liquid to sprout 1. a kind of promotion Radix Baccaureae ramiflorae cuttage branch is taken root, it is characterised in that the taking root liquid is by following quality The each component composition of fraction：50~500 ‰ methyl α-naphthyl acetates, 300~900 ‰ indolebutyric acids, 5~10% sucrose, 0.3~0.9% KMnO4, 0.5~5% ethanol.
- 2. taking root liquid according to claim 1, it is characterised in that the taking root liquid is grouped by each group of following mass fraction Into：100~400 ‰ methyl α-naphthyl acetates, 400~700 ‰ indolebutyric acids, 6~8% sucrose, 0.6~0.8% KMnO4, 1~5% ethanol.
- 3. taking root liquid according to claim 1, it is characterised in that the taking root liquid is grouped by each group of following mass fraction Into：300 ‰ methyl α-naphthyl acetates, 600 ‰ indolebutyric acids, 7% sucrose, 0.7% KMnO4, 2% ethanol.
- 4. claims 1 to 3 any one of them taking root liquid is taken root the application in sprouting promoting Radix Baccaureae ramiflorae cuttage branch.
- A kind of method to sprout 5. promotion Radix Baccaureae ramiflorae cuttage branch is taken root, it is characterised in that comprise the following steps：S1. 1~3 year raw branch on ripe Radix Baccaureae ramiflorae maternal plant is chosen as cuttage branch, the rugosity of branch is 1.0~3.0cm, Length is 20~25cm；Burnt immediately after cutting and seal and wrapped up with preservative film, removed whole branch after leaf with preservative film Parcel；The bottom 1cm of branch sealing to be cut before cuttage, then branch length is cut into 15cm, branch base portion is cut to 15 ° of angle shapes, Then branch is immediately placed in any one of claims 1 to 3 taking root liquid and soaks 2h, the soaking depth of branch bottom is 7cm；S2. by the branch cutting after being soaked in S1 in by the sand bed disinfected, towards south to 60 ° of oblique cuttings, the row of cuttage It is 5~7cm away from the depth for 20~22cm, insertion；After cuttage cutting is sprayed with wide-spectrum bactericide；Covered above sand bed at 1m First layer shading net, shading rate are 60%；Second layer shading net is set again above sand bed at 2m, and shading rate is 60%, is carried out double-deck Shading；First layer shading net is removed after 90d；S3. watering 2 times daily after cuttage；Weekly branch is sprayed with wide-spectrum bactericide 1 time；Each branch is sprayed every 15 days 100~120mL nutrient solutions；After 50d, 50~100mL taking root liquids are poured to each branch every 10d.
- 6. according to the method described in claim 5, it is characterized in that, sand bed described in S2 is disinfected to be with mass fraction 0.4~1.0% KMnO4Solution carries out disinfection, and be exposed to the sun 2d.
- 7. according to the method described in claim 5, it is characterized in that, branch described in S1 is life in 2 years, the rugosity of branch is 2.0cm, length 25cm.
- 8. according to the method described in claim 5, it is characterized in that, the dosage of taking root liquid described in S4 is 100mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108781814A (en) * | 2018-06-27 | 2018-11-13 | 云南省热带作物科学研究所 | A kind of beautiful different germ plasm resources strange land sprouting and rooting method of promise |
| CN112154840A (en) * | 2020-08-20 | 2021-01-01 | 广西南亚热带农业科学研究所 | Cultivation method of elaeagnus conferta roxb pot culture |
| CN113519533A (en) * | 2021-07-14 | 2021-10-22 | 湛江市三岭山森林公园管理处 | Nepenthes rooting liquid and application thereof in promoting rapid rooting propagation of nepenthes cutting branches |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264536A (en) * | 2000-03-21 | 2000-08-30 | 张荣江 | Regeneration promoter for plant |
| CN1775030A (en) * | 2005-12-06 | 2006-05-24 | 西南大学 | Efficient water soluble root-inducing powcler for taxus media |
| CN103416434A (en) * | 2013-07-12 | 2013-12-04 | 江苏农景生态建设有限公司 | Plant rooting agent and preparation method thereof |
-
2017
- 2017-11-17 CN CN201711147720.0A patent/CN107897182B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264536A (en) * | 2000-03-21 | 2000-08-30 | 张荣江 | Regeneration promoter for plant |
| CN1775030A (en) * | 2005-12-06 | 2006-05-24 | 西南大学 | Efficient water soluble root-inducing powcler for taxus media |
| CN103416434A (en) * | 2013-07-12 | 2013-12-04 | 江苏农景生态建设有限公司 | Plant rooting agent and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 张洪昌等: "《植物生长调节剂使用手册》", 31 January 2015, 中国农业出版社 * |
| 湖南省教育委员会编: "《全国农民中等专业学校试用教材 林木栽培学》", 31 July 1986, 湖南教育出版社 * |
| 王庆菊等: "《园林苗木繁育技术》", 30 September 2014, 中国农业大学出版社 * |
| 王涛等: "《ABT生根粉应用技术手册》", 31 May 1989, 中国林业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108781814A (en) * | 2018-06-27 | 2018-11-13 | 云南省热带作物科学研究所 | A kind of beautiful different germ plasm resources strange land sprouting and rooting method of promise |
| CN112154840A (en) * | 2020-08-20 | 2021-01-01 | 广西南亚热带农业科学研究所 | Cultivation method of elaeagnus conferta roxb pot culture |
| CN112154840B (en) * | 2020-08-20 | 2021-11-09 | 广西南亚热带农业科学研究所 | Cultivation method of elaeagnus conferta roxb pot culture |
| CN113519533A (en) * | 2021-07-14 | 2021-10-22 | 湛江市三岭山森林公园管理处 | Nepenthes rooting liquid and application thereof in promoting rapid rooting propagation of nepenthes cutting branches |
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