CN1757303A - Biological yellow storage agent for stalk forage - Google Patents
Biological yellow storage agent for stalk forage Download PDFInfo
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- CN1757303A CN1757303A CNA2005100447482A CN200510044748A CN1757303A CN 1757303 A CN1757303 A CN 1757303A CN A2005100447482 A CNA2005100447482 A CN A2005100447482A CN 200510044748 A CN200510044748 A CN 200510044748A CN 1757303 A CN1757303 A CN 1757303A
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- China
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- storage agent
- cfu
- yellow storage
- biological
- glucanase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
A biologic leaven for storage of straw-stalk feed is proportionally prepared from the spray dried powder of Bacillus subtilis, the vacuum freeze-dried powder of lactobacillus plantarum, pentose pediococcus, cellulase, xylanase, beta-glucanase, citric acid, and glucose or cane sugar. It is applied through dissolving it in water by 1-50g/t.
Description
Technical field
The present invention relates to a kind of biological yellow storage agent of straw feed, belong to biological feedstuff leavening technical field.
Background technology
The stalk yellow storage agent is exactly the composite biological additive that adds microorganism and enzyme in stalk, acting in conjunction by these beneficial microbes and enzyme, make in the stalk fermentation process beneficial microbe status of having the advantage all the time, and harmful assorted bacterium is suppressed, it is soft to make the yellow dry straw that is not suitable for ensiling originally also can become quality by the sealing storage, moistening bulk, the fragrant agreeable to the taste cattle and sheep roughage of acid.The yellow storage of stalk mainly is at the low wheat straw of water content, straw and the half yellow or yellow corn stalk of doing, the stalk of unsuitable ensiling such as sorghum straw, cellulose in this class stalk is aging, thick and stiff, nutrient composition content is also low, the animal palatability is very poor, during with such straw ensiling since the respiration of stalk own almost stop, be difficult to cause anaerobic environment by the respiration of stalk own, the lactic acid bacterium number that adsorbs on the plant significantly reduces in addition, soluble sugar content seldom, impel the environment of lactic acid bacteria breeding also not possess, the ensiling quality can't guarantee, can only be used for yellow storage.This yellow storage technique is actually the development of ensiling technology, more emphasizes the effect of enzyme.
China has obtained certain achievement in the silage additive research field, and retrieval can look into that to have declared patent multinomial, but yellow storage bio-additive does not still have patent application.Patent applied for mostly is lactic acid bacteria or lactic acid bacteria and complex enzyme mixture, be mainly used in stalk moisture greater than 50% green forage, optimum moisture is 60～70%, but is unsuitable for the processing of moisture at the half-dried even dry straw below 40%, and the microorganism of being added and enzyme are not suitable for yellow storage.And under real service condition, rural area casual household especially, it is bigger to collect dark green stalk difficulty, mostly is half-dried or dry straw, owing to lack the treatment additive of this type of stalk, seldom is used for feed, and burn wasted more.Therefore study a kind of yellow storage bio-additive and just become very important problem.
Summary of the invention
The biological yellow storage agent that the purpose of this invention is to provide a kind of straw feed contains the cellulase in genetic engineering Pichia yeast source, the zytase and the 1,4 beta-glucanase in aspergillus niger source, and Lactobacillus plantarum, Pediococcus pentosaceus, bacillus subtilis.According to the low characteristic of plant cell breath stopped, moisture of Huang storage stalk, oxygen consumption microorganism bacillus subtilis is added so that faster consumption oxygen forms anaerobic environment; According to the design feature of stalk, be purpose with the soluble sugar yield of maximum, composite cellulase, zytase and 1,4 beta-glucanase.This preparation is suitable for the Huang storage of moisture at the half-dried or dry straw below 40%, has the characteristics of enzyme reasonable mixture ratio alive, the coexistence of anaerobic-aerobic microorganism.
The objective of the invention is to realize with following technical scheme:
The composition of this biological yellow storage agent: bacillus subtilis spray dried powder, 1～5 * 10
8Cfu/g; Lactobacillus plantarum vacuum freeze drying powder, 1～3 * 10
9Cfu/g; Pediococcus pentosaceus 5～9 * 10
9Cfu/g; Cellulase, 2000～5000U/g; Zytase, 3000～5000IU/g; 1,4 beta-glucanase, 1000～3000U/g; Citric acid 10～20%; All the other are glucose or sucrose.
Product 100% of the present invention passes through the accurate sub-sieve of 80 targets by the accurate sub-sieve of 40 targets more than 80%; Moisture 5～10%; Use soluble in water, use amount 1～50g/t.
Biological yellow storage agent of the present invention contains aerobic bacillus, amphimicrobian yeast-like fungi Pediococcus pentosaceus and the whole zymophyte Lactobacillus plantarum of starting, contain cellulase, zytase, the 1,4 beta-glucanase of high temperature resistant (80 ℃ of active preservations are more than 80%) and acidproof (pH4.0～6.5 are active preserves more than 80%), contain the citric acid of regulating acidity and the carbohydrate that inspires ferment.Be suitable for the Huang storage of moisture at the half-dried or dry straw below 40%.
Below in conjunction with embodiment the present invention is described in further details:
Embodiment 1: bacillus subtilis spray dried powder, 1 * 10
8Cfu/g; Lactobacillus plantarum vacuum freeze drying powder, 1 * 10
9Cfu/g; Pediococcus pentosaceus 5 * 10
9Cfu/g; Cellulase, 2000U/g; Zytase, 3000IU/g; 1,4 beta-glucanase, 1000U/g; Citric acid 10%; All the other are glucose.Use soluble in water, use amount 50g/t.The yellow storage of pocket type is 30 days under the normal temperature, and acidization is obviously accelerated than control group, putrefactive phenomenon do not occur, and NDF and ADF amount reduce, the yellow storage successfully.
Embodiment 2: bacillus subtilis spray dried powder, 5 * 10
8Cfu/g; Lactobacillus plantarum vacuum freeze drying powder, 3 * 10
9Cfu/g; Pediococcus pentosaceus 9 * 10
9Cfu/g; Cellulase, 5000U/g; Zytase, 5000IU/g; 1,4 beta-glucanase, 3000U/g; Citric acid 20%; All the other are sucrose.Use soluble in water, use amount 1g/t.The yellow storage of pocket type is 30 days under the normal temperature, and acidization is obviously accelerated than control group, putrefactive phenomenon do not occur, and NDF and ADF amount reduce, the yellow storage successfully.
Claims (2)
- A kind of biological yellow storage agent of straw feed is characterized in that:1, the composition of this biological yellow storage agent: bacillus subtilis spray dried powder, 1～5 * 10 8Cfu/g; Lactobacillus plantarum vacuum freeze drying powder, 1～3 * 10 9Cfu/g; Pediococcus pentosaceus 5～9 * 10 9Cfu/g; Cellulase, 2000～5000U/g; Zytase, 3000～5000IU/g; 1,4 beta-glucanase, 1000～3000U/g; Citric acid 10～20%; All the other are glucose or sucrose.Use soluble in water, use amount 1～50g/t.
- 2, this biological yellow storage agent contains aerobic bacillus, amphimicrobian yeast-like fungi Pediococcus pentosaceus and the whole zymophyte Lactobacillus plantarum of starting, contain cellulase, zytase, the 1,4 beta-glucanase of high temperature resistant (80 ℃ of active preservations are more than 80%) and acidproof (pH4.0～6.5 are active preserves more than 80%), contain the citric acid of regulating acidity and the carbohydrate that inspires ferment.Be suitable for the Huang storage of moisture at the half-dried or dry straw below 40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100447482A CN1757303A (en) | 2005-09-22 | 2005-09-22 | Biological yellow storage agent for stalk forage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100447482A CN1757303A (en) | 2005-09-22 | 2005-09-22 | Biological yellow storage agent for stalk forage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1757303A true CN1757303A (en) | 2006-04-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005100447482A Pending CN1757303A (en) | 2005-09-22 | 2005-09-22 | Biological yellow storage agent for stalk forage |
Country Status (1)
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| CN (1) | CN1757303A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878859A (en) * | 2010-07-01 | 2010-11-10 | 西北农林科技大学 | Microbial additive for silage fodder and preparation method thereof |
| CN102783561A (en) * | 2012-07-30 | 2012-11-21 | 江南大学 | Preparation method for energy-saving biological straw feed |
| CN103652508A (en) * | 2013-12-20 | 2014-03-26 | 南京农业大学 | Ensilage combination including barley straws and festuca arundinacea and application thereof |
| CN103844002A (en) * | 2014-03-26 | 2014-06-11 | 金乡县大蒜研究所 | Preparation method of whole garlic straw silage |
| CN103947909A (en) * | 2014-03-31 | 2014-07-30 | 宁夏乙征生物工程有限公司 | Enzyme process yellow ensilage method of corn straws |
| CN105341325A (en) * | 2015-11-20 | 2016-02-24 | 中国农业科学院饲料研究所 | Enzyme bacterium composite additive used for fermentation of straw green/yellow silage |
| CN105724760A (en) * | 2016-02-19 | 2016-07-06 | 中国农业科学院饲料研究所 | Crop straw-fermented feed and preparation method and application thereof |
| CN109123092A (en) * | 2018-09-10 | 2019-01-04 | 李开明 | The preparation method of Huang storage straw feed |
| CN110122672A (en) * | 2019-06-02 | 2019-08-16 | 吉林省农业科学院 | A kind of corn stover Huang storage synergist and preparation method |
| CN115039833A (en) * | 2022-05-23 | 2022-09-13 | 中国科学院东北地理与农业生态研究所 | Preparation method of yellow-stored straw feed by-10 ℃ non-freezing bacterium-enzyme synergistic fermentation |
| CN115669809A (en) * | 2022-10-17 | 2023-02-03 | 湖南农业大学 | Corn silage additive |
-
2005
- 2005-09-22 CN CNA2005100447482A patent/CN1757303A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878859A (en) * | 2010-07-01 | 2010-11-10 | 西北农林科技大学 | Microbial additive for silage fodder and preparation method thereof |
| CN102783561A (en) * | 2012-07-30 | 2012-11-21 | 江南大学 | Preparation method for energy-saving biological straw feed |
| CN103652508A (en) * | 2013-12-20 | 2014-03-26 | 南京农业大学 | Ensilage combination including barley straws and festuca arundinacea and application thereof |
| CN103844002A (en) * | 2014-03-26 | 2014-06-11 | 金乡县大蒜研究所 | Preparation method of whole garlic straw silage |
| CN103947909A (en) * | 2014-03-31 | 2014-07-30 | 宁夏乙征生物工程有限公司 | Enzyme process yellow ensilage method of corn straws |
| CN103947909B (en) * | 2014-03-31 | 2016-01-13 | 宁夏乙征生物工程有限公司 | A kind of yellow storage method of enzyme process of maize straw |
| CN105341325A (en) * | 2015-11-20 | 2016-02-24 | 中国农业科学院饲料研究所 | Enzyme bacterium composite additive used for fermentation of straw green/yellow silage |
| CN105724760A (en) * | 2016-02-19 | 2016-07-06 | 中国农业科学院饲料研究所 | Crop straw-fermented feed and preparation method and application thereof |
| CN109123092A (en) * | 2018-09-10 | 2019-01-04 | 李开明 | The preparation method of Huang storage straw feed |
| CN110122672A (en) * | 2019-06-02 | 2019-08-16 | 吉林省农业科学院 | A kind of corn stover Huang storage synergist and preparation method |
| CN115039833A (en) * | 2022-05-23 | 2022-09-13 | 中国科学院东北地理与农业生态研究所 | Preparation method of yellow-stored straw feed by-10 ℃ non-freezing bacterium-enzyme synergistic fermentation |
| CN115039833B (en) * | 2022-05-23 | 2023-10-20 | 中国科学院东北地理与农业生态研究所 | Preparation method of non-icing bacterial enzyme synergistic fermentation yellow storage straw feed at-10 DEG C |
| CN115669809A (en) * | 2022-10-17 | 2023-02-03 | 湖南农业大学 | Corn silage additive |
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