CN1736167A - Maize restoring type male flower sterile cytoplasm hybrid seed production method - Google Patents
Maize restoring type male flower sterile cytoplasm hybrid seed production method Download PDFInfo
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- CN1736167A CN1736167A CN 200510012749 CN200510012749A CN1736167A CN 1736167 A CN1736167 A CN 1736167A CN 200510012749 CN200510012749 CN 200510012749 CN 200510012749 A CN200510012749 A CN 200510012749A CN 1736167 A CN1736167 A CN 1736167A
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- 240000008042 Zea mays Species 0.000 title claims abstract description 47
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 47
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title claims abstract description 26
- 235000009973 maize Nutrition 0.000 title claims abstract description 26
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 210000000805 cytoplasm Anatomy 0.000 title abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 21
- 235000005822 corn Nutrition 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000002955 isolation Methods 0.000 claims abstract 2
- 230000008774 maternal effect Effects 0.000 claims description 19
- 206010021929 Infertility male Diseases 0.000 claims description 14
- 208000007466 Male Infertility Diseases 0.000 claims description 14
- 238000011084 recovery Methods 0.000 claims description 14
- 230000009466 transformation Effects 0.000 claims description 14
- 208000000509 infertility Diseases 0.000 claims description 10
- 230000036512 infertility Effects 0.000 claims description 10
- 208000021267 infertility disease Diseases 0.000 claims description 10
- 238000009331 sowing Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 5
- 230000001086 cytosolic effect Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 238000000844 transformation Methods 0.000 claims description 2
- 240000000233 Melia azedarach Species 0.000 abstract 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 238000009402 cross-breeding Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000010977 jade Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 235000010663 Lavandula angustifolia Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 239000001102 lavandula vera Substances 0.000 description 1
- 235000018219 lavender Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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Abstract
The invention relates to a maize restoring type male flower sterile cytoplasm hybrid seed production method. The method comprises the following steps: (1) transferring the female parent inbred line Yong 31257 of the conventional corn hybrid Yongyu No. 3 into a C-type Es subspecies male flower sterile line Es Yong 31257; (2) transferring the male parent inbred line Lian 1538 of the conventional corn hybrid Yongyu No. 3 into a restorer line Hulian 1538 of the C-type sterile line; (3) in the seed production field with male sterile line Es Yong 31257 as female parent and restoring line Hulian 1538 as male parent, the female parent is not emasculated to obtain sterile Yongyu No. 3 corn hybrid seed with normal flowering, pollen dispersing and seed setting. The invention is characterized in that the seed production field does not need to be emasculated manually, and other fields such as an isolation area are the same as the conventional corn hybrid Yongyu No. 3. The method has the advantages of high seed purity, high yield and low seed production cost, and is suitable for large-area popularization and application.
Description
Technical field
The present invention relates to a kind of producing method for seed of corn recovery type male-sterility cytoplasmic hybrid kind, be specifically related to a C type Es subspecies corn male-sterility system, a C type corn recovers the producing method for seed of system and maize sterility hybrid thereof.
Background technology
Corn seed producing practice for many years shows, ordinary maize cross breeding seed field, and maternal artificial emasculation is difficult to thoroughly clean, and maternal strain selfing and sisters hand over phenomenon to be difficult to avoid, and often castrate because of female parent and do not cause impurity of seeds only, and purity reduces, and causes crossbreed (F
1) underproduction.Now, guarantee that the most effective measure of corn hybrid seed seed purity is to utilize the preparing maize sterility hybrid seed technology.The preparing maize sterility hybrid seed Study on Technology is utilized, and the U.S. just began as far back as nineteen thirties; The former Soviet Union just extensively utilizes before disintegrating; China because of various reasons, and is later to this Study on Technology, influenced the corn hybrid advantage and used in agricultural production.
Summary of the invention
The purpose of this invention is to provide a kind of sterilization producing method for seed of guaranteeing beautiful forever No. 3 seed purities of corn hybrid seed, or be called a kind of corn recovery type male-sterility cytoplasmic hybrid kind producing method for seed.
Maize sterility is that (T type, S type, C type) and maintenance line thereof and male parent are recovered system and formed by maternal male-sterility.Research practice shows, the transformation of a certain ordinary maize inbred line is become male-sterility system, and than being easier to, five, six generations can finish.Yet, become the recovery system of male-sterility system difficult a certain inbred line transformation, eight, nine generations of minimum need just can finish; If be used for producing, also needing needs two, three generations just can finish again by hybridization, evaluation, screening, breeding supervisor.Therefore, many breedings man hopes and draws back.
The inventor has finished a kind of producing method for seed of maize sterility hybrid through concentrating on studies for many years.Design of the present invention is: beautiful forever No. 3 that cultivate in recent years is the ordinary maize crossbreed, and maternal inbred line is forever 31257, and male parent is to connect 1538.This crossbreed is tested and production test in China North China, east and northwest national region, obvious effect of increasing production, and the highest per mu yield reaches 1233.2 kilograms, is a quite rising fine corn seeds.The deficiency that this corn variety exists is that his father, female parent are conventional inbred line, and maternal plant is higher, and seed farm artificial emasculation inconvenience, seed purity are difficult to ensure.The present invention promptly provides a kind of sterilization producing method for seed of beautiful No. 3 corn hybrid seeds forever, to overcome the defective of prior art.
Particularly, method of the present invention may further comprise the steps:
(1) Es 31257 transformation forever
It is Es forever 31257 that maternal inbred line 31257 transformations forever of beautiful forever No. 3 of ordinary maize crossbreed are become C type Es subspecies male-sterility;
(2) recovery of C type is the transformation of extensive company 1538
The male parent inbred line of beautiful forever No. 3 of ordinary maize crossbreed is connected 1538, and it is extensive company 1538 that transformation becomes the recovery of C type male sterile line;
(3) beautiful forever No. 3 maize hybrid seed productions of sterilization
With male-sterility is that Es 31257 makes female parent forever, and recovery is the seed farm that extensive company 1538 makes male parent, and female parent is not castrated, and beautiful forever No. 3 seeds of the solid maize sterility hybrid of loose powder obtain normally to bloom.
The feature of No. 3, jade is identical forever to utilize beautiful forever No. 3 corn hybrid seeds of sterilization that producing method for seed of the present invention obtains and ordinary maize crossbreed.
The technique effect that the present invention obtains is:
1, reduced breeding cost: ordinary maize crossbreed (containing beautiful No. 3 forever, down together) seed farm, castrating for every mu needs to finish in about about 7 days with 2 workers.The beautiful forever No. 3 maize hybrid seed production Tian Yin of sterilization can save a large amount of manpower and financial resources without artificial emasculation, in 10,000 mu of seed farms, can save 20,000 of recruitments, by 30 yuan of daily wages, can save 600,000 yuan.
2, seed purity height: the sterilization seed farm, maternal inbred line WUHUAFEN self does not have solid ability, and the seed that plant is tied all is seeds of accepting the paternal pollen insemination, the seed purity height.
3, seed farm output height: ordinary maize cross breeding seed field, generally to pull out 1-3 sheet leaf simultaneously when manually taking out hero because of female parent, influence photosynthesis, unfavorable kernel grouting.The preparing maize sterility hybrid seed field, because maternal male flower WUHUAFEN, damaged blade not again, nutrient abundance during kernel grouting, full seed, grain are big, and test shows that its output can improve about 10%.
4, crossbreed (F
1) the output height: production practices show, general corn hybrid seed production field has the self-crossing seedling of 3-8% approximately, and it is very weak grow, cover time in that high stalk plant is strongly fragrant, and are shaky substantially.Maize sterility hybrid production field does not have self-crossing seedling, the growth neat and consistent, and per unit area yield can improve about 5%.
Description of drawings
Fig. 1 is Es type male sterile line 31257 transformation schematic diagrames forever.
Fig. 2 is that the C type recovers even 1538 transformation schematic diagrames of system.
Embodiment
Following examples are in order to explanation the present invention.
Embodiment
The sterilization that present embodiment the provides producing method for seed of beautiful No. 3 corn hybrid seeds forever comprises the steps:
(1) Es 31257 transformation forever
Referring to Fig. 1, Xiali in 2000 is done maternal with C type Es subspecies male sterile line C53,31257 make male parent forever, forms C53 * forever 31257, and the winter is sowed its F in Hainan then
1Seed, taking out male loose powder phase, to select the male-sterility strain to do maternal, with 31257 backcrossing the F that obtains as male parent forever
1Seed in the calendar year 2001 summer sowing, selects sterile strain with 31257 backcrossing forever again, and continuous backcross is 5 times like this, breeds Es forever 31257;
(2) recovery of C type is the transformation of extensive company 1538
Referring to Fig. 2, in winter calendar year 2001, it is maternal to select for use C type male sterile line C53 to do, and it is that 314-3 makes male parent forever that the C type recovers, and forms C53 * combination of 314-3 single cross forever, its F
1In summer sowing in 2002, do male parent and its hybridization with connecting 1538 again, form (C53 * 314-3) forever * connect 1,538 three and hand over and make up, be called for short extensive C and connect 1538
1
Winter in 2002, extensive C connected 1538 in Hainan
1Respectively broadcast 1 row with company 1538, extensive C connects 1538
1The 3 strain tassels loose powder of normally blooming is arranged on the row, respectively with connect 1538 selfing strain pairing backcrossings (hybridization), promptly extensive C connects 1538
1-1 * connect 1538-1, be called for short extensive C and connect 1538
2-1; Extensive C connects 1538
1-2 * connect 1538-2, be called for short extensive C and connect 1538
2-2; Extensive C connects 1538
1-3 * connect 1538-3, be called for short extensive C and connect 1538
2-3;
Summer in 2003, extensive C connects 1538
2Connect 1538-1 corresponding with it such as-1 grade etc. respectively broadcasts 1 and goes.Take out the male loose powder phase and identify that extensive C connects 1538
2-1 and extensive C connect 1538
2-3 recovery strain rates are few, eliminate; Extensive C connects 1538
2-2 recover strain rate height, middle choosing.In the present age, extensive C connects 1538
2-2 recovery strain is done maternal, respectively with the selfing strain pairing backcrossing that connects 1538-2, has middlely selected 3 pairs.The rest may be inferred, finishes 5 times in the spring in 2004 and backcross.Then, will backcross 5 times middle roguing, 2 generations of selfing separation screening again,, successively go through 9 generations (annual 2-3 generation) to the summer in 2005, breed the C type and recover system and connect 1538, be called for short extensive company 1538 (or forever 1538);
(3) beautiful forever No. 3 hybrid maize seed production methods of sterilization: 31257 make female parent forever with Es, extensive company 1538 makes male parent, and seed farm mother, male parent row ratio are 4: 1, and it is maternal promptly to broadcast 4 row, broadcasts 1 row male parent, alternate plantation.
Planting density is 4500 strain/mus.Broadcast female parent during sowing earlier, male parent is divided the second phase sowing, the first phase and the maternal sowing same period, second phase late sowing 3-5 days.The maternal tasseling stage of producing method for seed of the present invention is not castrated, and other is provided with as isolated area and waits that No. 3, jade is identical forever with the ordinary maize crossbreed.
Sterilization is parent's feature of beautiful No. 3 corn hybrid seeds forever:
1, Es forever 31257: about 100 days breeding times.Seedling leaf sheath purple.About plant height 200cm (not containing tassel), about the 168cm of fringe position, 17-18 sheet leaf, leaf and stem stalk angle are little on the upper fringe, and the leaf attitude is straight on the upper fringe, the plant type compactness.The tassel WUHUAFEN, sterility is stable, and the tassel main shaft is long, obvious, and the tassel side shoot is few, little, and attitude is straight.Female Honoka silk is dark green.Fruit ear is cylindrical, spike length 18-20cm, and seed is arranged straight, the capable grain of 10-12, red axle.Grain is big, yellow, inclined to one side solids type, thousand kernel weight 360-426 gram.
2, extensive company 1538: about 100 days breeding times of summer sowing.Seedling leaf sheath lavender, first blade tip circle, the leaf margin green of the 4th break, about plant height 200cm, about the 85cm of fringe position, 21 leaves, strain shape is compact, and the tassel branch is more, and main shaft is obvious, flower pesticide yellow, sterile lemma green.Filigree is dark green.Fruit ear is short and thick, about spike length 15cm, about the thick 5.5cm of fringe, about a tassel row number 22 row.
Claims (4)
1, a kind of corn recovery type male-sterility cytoplasmic hybrid kind producing method for seed, it is characterized in that: comprise that a C type Es subspecies male-sterility system does maternal, a C type recovers system and makes male parent, the row of taking to suit in secure isolation zone compares, suitable planting density, female parent such as does not castrate at supplementary measures, and step is as follows:
(1) Es 31257 transformation forever
It is Es forever 31257 that maternal inbred line 31257 transformations forever of beautiful forever No. 3 of ordinary maize crossbreed are become C type Es subspecies male-sterility;
(2) recovery of C type is the transformation of extensive company 1538
The male parent inbred line of beautiful forever No. 3 of ordinary maize crossbreed is connected 1538, and it is extensive company 1538 that transformation becomes the recovery of C type male sterile line;
(3) beautiful forever No. 3 maize hybrid seed productions of sterilization
With male-sterility is that Es 31257 makes female parent forever, and recovery is the seed farm that extensive company 1538 makes male parent, and female parent is not castrated, and beautiful forever No. 3 seeds of the solid maize sterility hybrid of loose powder obtain normally to bloom.
2, by the described producing method for seed of claim 1, it is characterized in that: 31257 make female parent forever with Es, extensive company 1538 makes male parent, and seed farm mother, male parent row ratio are 4: 1, and it is maternal promptly to broadcast 4 row, broadcasts 1 row male parent, alternate plantation.
3, by the described producing method for seed of claim 1, it is characterized in that: 31257 make female parent forever with Es, extensive company 1538 makes male parent, and the planting density of the field production of hybrid seeds is 4500 strain/mus.
4, by the described producing method for seed of claim 1, it is characterized in that: 31257 make female parent forever with Es, extensive company 1538 makes male parent, broadcasts female parent earlier, and male parent is divided the sowing of two phases, the first phase and the maternal sowing same period, second phase late sowing 3-5 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200510012749 CN1736167A (en) | 2005-08-22 | 2005-08-22 | Maize restoring type male flower sterile cytoplasm hybrid seed production method |
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| CN 200510012749 CN1736167A (en) | 2005-08-22 | 2005-08-22 | Maize restoring type male flower sterile cytoplasm hybrid seed production method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173216A (en) * | 2017-06-12 | 2017-09-19 | 甘肃农业大学 | Seed selection and its application in Mixed plant that corn difference pericarp color CMS tri- is |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173216A (en) * | 2017-06-12 | 2017-09-19 | 甘肃农业大学 | Seed selection and its application in Mixed plant that corn difference pericarp color CMS tri- is |
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