CN1711352A - 丙型肝炎病毒复制的高允许性细胞系 - Google Patents

丙型肝炎病毒复制的高允许性细胞系 Download PDF

Info

Publication number
CN1711352A
CN1711352A CN200380103205.2A CN200380103205A CN1711352A CN 1711352 A CN1711352 A CN 1711352A CN 200380103205 A CN200380103205 A CN 200380103205A CN 1711352 A CN1711352 A CN 1711352A
Authority
CN
China
Prior art keywords
hcv
cell
clone
cells
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200380103205.2A
Other languages
English (en)
Chinese (zh)
Inventor
C·M·赖斯三世
K·J·布莱特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Original Assignee
University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington filed Critical University of Washington
Publication of CN1711352A publication Critical patent/CN1711352A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24221Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/24011Flaviviridae
    • C12N2770/24211Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24251Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN200380103205.2A 2002-11-13 2003-11-13 丙型肝炎病毒复制的高允许性细胞系 Pending CN1711352A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42625602P 2002-11-13 2002-11-13
US60/426,256 2002-11-13

Publications (1)

Publication Number Publication Date
CN1711352A true CN1711352A (zh) 2005-12-21

Family

ID=32313126

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200380103205.2A Pending CN1711352A (zh) 2002-11-13 2003-11-13 丙型肝炎病毒复制的高允许性细胞系

Country Status (8)

Country Link
US (1) US7455969B2 (https=)
EP (1) EP1578956A4 (https=)
JP (1) JP2006506074A (https=)
CN (1) CN1711352A (https=)
AU (1) AU2003294292A1 (https=)
CA (1) CA2505551A1 (https=)
MX (1) MXPA05005092A (https=)
WO (1) WO2004044182A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104313038A (zh) * 2008-12-26 2015-01-28 东丽株式会社 衍生自丙型肝炎病毒的核酸以及通过使用该核酸各自制备的表达载体、转化细胞和丙型肝炎病毒颗粒

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7794942B2 (en) * 1997-03-04 2010-09-14 Washington University Cell lines permissive for HCV replication
US7790448B2 (en) 2003-09-19 2010-09-07 Tokyo Metropolitan Organization For Medical Research Nucleic acid and gene derived from novel HCV strain and replicon-replicating cell using said gene
CA2539457C (en) 2003-09-19 2013-12-03 Tokyo Metropolitan Organization For Medical Research Nucleic acid and gene derived from novel hcv strain and replicon-replicating cell using said gene
US7659103B2 (en) * 2004-02-20 2010-02-09 Tokyo Metropolitan Organization For Medical Research Nucleic acid construct containing fulllength genome of human hepatitis C virus, recombinant fulllength virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus Particle
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
EP1909564A4 (en) 2005-07-18 2010-06-30 Novartis Ag SMALL ANIMAL MODEL FOR HCV REPLICATION
EP1910522A2 (en) * 2005-07-18 2008-04-16 Novartis Vaccines and Diagnostics S.r.l. Caveolin-3 and in vitro culture of hepatitis c virus
JP5035985B2 (ja) * 2005-09-30 2012-09-26 国立感染症研究所長 新規組換え型ヒトc型肝炎ウイルス様粒子とその産生方法
US7504255B2 (en) * 2006-08-17 2009-03-17 Saint Louis University Compositions and methods for generation of infectious hepatitis C virus in immortalized human hepatocytes
ITMI20061772A1 (it) * 2006-09-18 2008-03-19 Consiglio Nazionale Ricerche Procedimento per la preparazione di cellule epatiche da cellule progenitrici presenti nel sangue di cordone ombelicale
CA2780079A1 (en) * 2009-11-18 2011-05-26 Basf Plant Science Company Gmbh Process for the production of fine chemicals
KR102089477B1 (ko) 2012-08-31 2020-03-17 더 가버너스 오브 더 유니버시티 오브 알버타 1차 인간 간세포의 표현형을 갖는 세포를 생성하기 위한 방법 및 조성물

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6127116A (en) * 1995-08-29 2000-10-03 Washington University Functional DNA clone for hepatitis C virus (HCV) and uses thereof
DE19915178A1 (de) * 1999-04-03 2000-10-05 Univ Mainz Johannes Gutenberg Hepatitis C Virus Zellkultursystem
DK1296998T3 (da) * 2000-05-23 2011-12-12 Univ Washington HCV-varianter
EP1423522A2 (en) * 2001-01-23 2004-06-02 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Hepatitis c virus replicons and replicon enhanced cells
GB0114629D0 (en) 2001-06-15 2001-08-08 Pfizer Ltd Method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104313038A (zh) * 2008-12-26 2015-01-28 东丽株式会社 衍生自丙型肝炎病毒的核酸以及通过使用该核酸各自制备的表达载体、转化细胞和丙型肝炎病毒颗粒

Also Published As

Publication number Publication date
EP1578956A2 (en) 2005-09-28
US7455969B2 (en) 2008-11-25
EP1578956A4 (en) 2006-02-08
MXPA05005092A (es) 2005-07-01
US20060099595A1 (en) 2006-05-11
CA2505551A1 (en) 2004-05-27
WO2004044182A3 (en) 2005-03-10
JP2006506074A (ja) 2006-02-23
AU2003294292A1 (en) 2004-06-03
WO2004044182A2 (en) 2004-05-27

Similar Documents

Publication Publication Date Title
Blight et al. Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication
EP1801209B1 (en) Modified human hepatitis c virus genomic rna having autonomous replicative competence
CN1833024B (zh) 含有衍生自基因型2a丙肝病毒(HCV)基因组的核酸的核酸构建体和具有转移入其中的这样的核酸构建体的细胞
Appel et al. Efficient rescue of hepatitis C virus RNA replication by trans-complementation with nonstructural protein 5A
US8506969B2 (en) Efficient cell culture system for hepatitis C virus genotype 7a
US8026092B2 (en) Replication competent hepatitis C virus and methods of use
CN1711352A (zh) 丙型肝炎病毒复制的高允许性细胞系
US7655406B2 (en) Nucleotide sequences coding for the non-structural proteins of the hepatitis C virus
JP2011505159A (ja) Hcvns3プロテアーゼレプリコンシャトルベクター
CN104313038A (zh) 衍生自丙型肝炎病毒的核酸以及通过使用该核酸各自制备的表达载体、转化细胞和丙型肝炎病毒颗粒
CN101321865B (zh) 感染性丙型肝炎病毒颗粒高效生产体系
JPWO2005028652A1 (ja) 新規hcv株由来の核酸、遺伝子、及び該遺伝子を利用したレプリコン複製細胞
JPWO2011024875A1 (ja) C型肝炎ウイルスの新規株由来のポリヌクレオチド及びその利用
JP2009513109A (ja) カベオリン−3およびc型肝炎ウイルスのインビトロ培養物

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication