CN1695493A - Method for producing bean sprout rich in organic gallium and application - Google Patents
Method for producing bean sprout rich in organic gallium and application Download PDFInfo
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- CN1695493A CN1695493A CNA2004100261065A CN200410026106A CN1695493A CN 1695493 A CN1695493 A CN 1695493A CN A2004100261065 A CNA2004100261065 A CN A2004100261065A CN 200410026106 A CN200410026106 A CN 200410026106A CN 1695493 A CN1695493 A CN 1695493A
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Abstract
An organogallium enriched bean-sprout for preventing asteoporosis, hipertension, coronary heart disease and arteriosclerosis is prepared through immersing bean in the solution of gallium and sprouting and growing in it.
Description
Technical field:
The present invention relates to a kind of production method and application of bean sprouts, particularly, is a kind of production method and application thereof that contains the abundant bean sprouts of gallium.
Background technology:
The bean sprouts is a kind of common foods of people, and the Vit C contents of bean sprouts is higher than apple, pears etc., and hypertension, coronary heart disease and artery sclerosis can be prevented, diseases such as iron deficiency in often edible bean sprouts.Along with the raising of people's quality of life and the quickening of rhythm of life, traditional root bean sprouts that has progressively is eliminated, the various bean sprouts of supplying on the existing market are owing to added multiple additives in cultivating process, the rootless bean sprouts of producing, though output height, loose beautiful has increased many hidden danger for the people's health.The trace element gallium has positive effect in treatment in the osteopathy, but inorganic gallium (gallium nitrate, gallium chloride etc.) is not easy to be absorbed by the body, so consumption is very big, and heavy dose of inorganic gallium has side effect.
Summary of the invention:
In view of the shortcoming of the existence of above technology, main purpose of the present invention is to provide a kind of production method that contains abundant organic gallium, helps the bean sprout rich in organic gallium of absorption of human body.
Another object of the present invention is to utilize the application of bean sprout rich in organic gallium in preventing and treating osteoporosis.
For achieving the above object, the technical solution adopted in the present invention is:
A kind of production method of bean sprout rich in organic gallium is beans to be placed in 25 ℃ of-37 ℃ of temperature ranges of suitable growth germinate, and its special character is: this method is as follows:
Legume-seeds is put into the gallium (Ga that concentration is the 0.1mg/L-300mg/L scope
+ 3) solution soaked 12-24 hour, was gallium (Ga in the 0.1mg/L-300mg/L scope with soaking bean or pea with concentration
+ 3) solution germination, growth.
Above-mentioned usefulness concentration be the bean or pea soaked of the Gallium solution in the 0.1mg/L-300mg/L scope after germination, move into the deep bid growth, every day, spray concentration was the gallium (Ga in the 0.1mg/L-300mg/L scope
+ 3) solution growth 3-4 day.
Above-mentioned beans is mung bean or soya bean.
Powdery is dried, is ground in the bean sprouts of producing.
The application of bean sprout rich in organic gallium in preventing and treating osteoporosis, the organic gallium in the bean sprouts have the osteoporotic effect of control.
The present invention is with respect to prior art, and its advantage is as follows:
The present invention will be unfavorable for the micro-inorganic gallium of absorption of human body, by in the process of cultivating the bean sprouts, inorganic gallium is combined with amino acid in the bean sprouts, thereby be formed with the organic gallium that is beneficial to absorption of human body, in the process of the edible bean sprouts of daily people, micro-gallium is mended in the human body, made people that positive effect be arranged in preventing and treating osteoporosis.
The present invention becomes organic gallium with the inorganic gallium of trace element, is beneficial to absorption of human body, and has no side effect.
The specific embodiment:
The concrete grammar that the present invention cultivates the bean sprouts with Gallium solution is as follows: choosing the mung bean or the soya bean of high-quality, is the gallium (Ga of 300mg/L at 25-37 ℃ with concentration in temperature with bean or pea
+ 3) solution soaked 1 day, was the gallium (Ga of 300mg/L in concentration with the bean or pea of soaking again
+ 3) temperature is after germinateing under 37 ℃ the condition, it to be moved into deep bid growth in the solution, every day, spray concentration was the gallium (Ga of 300mg/L
+ 3) solution 1 time, in temperature growth 3-4 day under 25-37 ℃ the condition, promptly cultivate organic gallium bean sprouts; Above-mentioned gallium (Ga
+ 3) concentration range of solution all can cultivate organic gallium bean sprouts in the 0.1mg/L-300mg/L scope.The organic gallium bean sprouts of producing is dried, is ground into powdery, be prepared into the bean sprouts powder, also the bean sprouts powder can be made the convenient forms such as tablet, capsule or granule of using.
Following table is each position gallium content in the bean sprouts:
Each position gallium content of Semen Phaseoli radiati Germinatus rich in gallium and Semen Glycines Germinatus rich in gallium (μ g/g)
| Full bean sprouts powder gallium content (μ g/g) | Skin of beancurd gallium content (μ g/g) | Lobe leaf gallium content (μ g/g) | Stem root gallium content (μ g/g) | |
| No gallium mung bean sprouts | ??2.55 | ??2.55 | ??2.55 | ??2.55 |
| Semen Phaseoli radiati Germinatus rich in gallium | ??231.30 | ??958.5 | ??149.0 | ??149.0 |
| Semen Glycines Germinatus rich in gallium | ??78.17 | ??430.1 | ??16.90 | ??36.9 |
In order to verify of the effect of organic gallium, enumerate some laboratory reports below to the sclerotin pine:
1, experiment purpose: observe the unusual effect of bone, cartilage metabolism that organic gallium causes estrogen deficiency, for its clinical practice provides experiment and theoretical foundation.
2, material
2.1, animal used as test
110 of 8 monthly age adult female sd inbred rats (Xian Medical Univ's Experimental Animal Center provides, national A level animal used as test), body weight 220-270g.Be divided into three groups at random: Sham-operated control group (20), osteoporosis model group (70), the organic gallium control of sham-operation group (20).
2.2, osteoporosis model duplicates
All under the urethane intraperitoneal injection of anesthesia, the belly tincture of iodine, alcohol disinfecting are opened the abdominal cavity to whole rats.Sham-operated control group and sham-operation prophylactic treatment group are sewed up after opening and cutting a fritter fat behind the abdomen, and the osteoporosis model group is opened behind the abdomen and sewed up operation back warming (4 death) after the excision bilateral ovaries.Rat recovers after 4 days, enters experiment.Fed for 12 weeks with conventional feed (feed is by Xian Medical Univ's Experimental Animal Center preparation).
2.3, treatment grouping and therapeutic dose
Model copy enters treatment stage after becoming.Experiment is divided into 5 groups: sham-operation (normal control group), osteoporosis control group, the organic gallium treatment of low dosage group, the organic gallium treatment of high dose group.Normal control group (12), osteoporosis control group (16), the normal raising; The organic gallium treatment of low dosage group (16) gives 120 μ g/kg organic gallium, 1 time/day; The organic gallium treatment of high dose group (15) gives 360g/kg organic gallium, 1 time/day; Experiment was carried out for 12 weeks.
2.4, draw materials
Each treated animal enters metabolic cage collection twenty-four-hour urine before putting to death animal, puts to death through the arteria coeliaca blood drawing before putting to death, and gets animal bilateral hind leg and lumbar vertebra and rib.Urine and blood real-time test or put into low temperature water tank and preserve, bone are preserved or formalin fixed at low temperature water tank, liquid nitrogen respectively.
3 experimental index and method
3.1 the strength detection of bone: make three point bending test with Material Testing Machine and measure.Key step: rat right side femur is peeled off, thoroughly removed soft tissue and periosteum, place to be equipped with in-35 ℃ of refrigerators and survey.The testing machine loading velocity is the 2mm/ branch.And fixing indication spacing is 20mm.
3.2 the mensuration of bone mineral density
The single photon method: the femur that will peel off is put into water, is that measurement point is measured with the femur mid point, and the radiation method contrasts mutually.
3.3 the mensuration of element calcium, phosphorus, gallium in the bone: method is with the assay method of element in the blood.
3.4 bone is resurveyed fixed: comprise that bone weight in wet base, dry weight and ash are heavy.Be placed on dry 72 hours side dry weights in 40 ℃ of baking boxs with peeling off complete femur, put into 580 ℃ of muffle furnaces again, it is heavy that ash is surveyed in ashing in 25 hours.
3.5 statistical procedures
All data are all imported computer, test and methods analyst with SPSS For windows7.5 version software, and measurement data result is with mean+SD (X ± SD) expression.
4 use three months anaphases of organic gallium treatment
4.1 the bone strength determining result shows that the osteoporosis control group is compared with normal control group bone strength, its intensity significantly descend (P＜0.05).Two groups intensity level of organic gallium treatment has rising trend than the osteoporosis contrast.See the following form
Right femoral strength
| Group | Example number (n) | Maximum distortion (mm) | Destroy load (newton) |
| High gallium treatment group is organized in the low gallium treatment of control group osteoporosis control group | ?7 ?7 ? ?7 ?6 | ??1.55±0.07 ??1.44±0.12 ? ??1.57±0.13 ??1.58±0.13 | ??89.6±9.88 ??51.18±12.01 ? ??70.77±11.84 ??67..33±13.61 |
4.2 show with single photon and its femur density of CT pH-value determination pH result: normal control group and osteoporosis group density all have significant difference (P＜0.05) with two method measurement results, and osteoporosis group bone density obviously reduces.The CT value shows that two groups simple with organic gallium treatment group, and its density value and osteoporosis group also have rising trend (P=0.51).If sample increases, its difference may occur.From using single photon method measurement result, low gallium treatment group bone density osteoporosis also has rising trend (P=0.099).See the following form
Right femur density (distinct methods)
| Group | Example number (n) | ??BMD(g/cm 2) | The example number | The CT value |
| High gallium treatment group is organized in the low gallium treatment of control group osteoporosis control group | ?7 ?8 ? ?8 ?6 | ??0.556±0.056 ??0.474±0.076 ? ??0.549±0.095 ??0.479±0.073 | ??11 ??8 ? ??9 ??7 | ??1389.91±221.14 ??1142.43±104.03 ? ??1278.44±141.32 ??1301.57±156.66 |
4.3 calcium, phosphorus, gallium element assay result show that the osteoporosis control group is compared with the normal control group in the bone, bone calcium, phosphorus all have obvious decline (P＜0.05).Compare bone calcium with the osteoporosis control group rising trend is arranged though respectively organize calcium content of bone with the treatment of organic gallium, perhaps claiming bone calcium to lose has sluggish trend.Use organic gallium treatment and respectively organize all obviously risings (P＜0.01) of bone gallium content.See the following form
Thigh os in os element calcium, phosphorus, gallium content
| Group | Example number (n) | ??Ca(mg/g) | ??P(mg/g) | ??Ga(ug/g) |
| High gallium treatment group is organized in the low gallium treatment of control group osteoporosis control group | ?5 ?5 ? ?5 ?5 | ??192.59±33.38 ??129.04±24.33 ? ??157.23±21.75 ??164.99±43.14 | ??197.97±10.38 ??71.61±9.87 ? ??134.97±86.84 ??73.45±43.94 | ??0.53±0.44 ??0.27±0.15 ? ??6.38±0.8 ??8.73±1.02 |
Decide the result 4.4 rat femur weight in wet base, dry weight, ash are resurveyed: compare with body weight after standardization, the osteoporosis group is compared with normal and low gallium treatment group all reduction trend.See the following form.
Femur gravimetry result
| Group | Example number (n) | Weight in wet base/dry weight | Example number (n) | Dry weight/weight in wet base | Ash weight/body weight |
| Control group osteoporosis control group hangs down gallium treatment group | ??12 ??9 ? ? ??10 | ??0.0024±0.00022 ??0.00199±0.0002 ? ? ??0.00307±0.00024 | ??5 ??5 ? ? ??5 | ??0.00189±0.0002 ??0.00142±0.00021 ? ? ??0.00154±0.00018 | ??0.00061±0.00014 ??0.00053±0.00014 ? ? ??0.00058±0.00019 |
5 conclusions: organic gallium has inhibitory action to the osteoporosis that estrogen deficiency causes.
Thereby, from above-mentioned experiment, can draw, bean sprout rich in organic gallium has tangible effect in the osteoporotic process of control, and its consumption to human body is the organic gallium of every day in 100-500 μ g scope.
Claims (5)
1, a kind of production method of bean sprout rich in organic gallium is that beans is placed in 25 ℃ of-37 ℃ of temperature ranges of suitable growth, germinates, and it is characterized in that: this method is as follows:
Legume-seeds is put into the gallium (Ga that concentration is the 0.1mg/L-300mg/L scope
+ 3) solution soaked 12-24 hour, was gallium (Ga in the 0.1mg/L-300mg/L scope with soaking bean or pea with concentration
+ 3) solution germination, growth.
2, the production method of bean sprout rich in organic gallium according to claim 1, it is characterized in that: described is that the bean or pea soaked of the Gallium solution in the 0.1mg/L-300mg/L scope are after germination with concentration, the growth of immigration deep bid, every day, spray concentration was the gallium (Ga in the 0.1mg/L-300mg/L scope
+ 3) solution growth 3-4 day.
3, the production method of bean sprout rich in organic gallium according to claim 1 and 2 is characterized in that: described beans is mung bean or soya bean.
4, the production method of bean sprout rich in organic gallium according to claim 1 and 2 is characterized in that: powdery is dried, is ground in the bean sprouts of producing.
5, the application of production bean sprout rich in organic gallium according to claim 1 and 2 in preventing and treating osteoporosis, the organic gallium in the bean sprouts have the osteoporotic effect of control.
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| CNA2004100261065A CN1695493A (en) | 2004-05-12 | 2004-05-12 | Method for producing bean sprout rich in organic gallium and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2689783A2 (en) * | 2005-11-01 | 2014-01-29 | Mount Sinai School of Medicine of New York University | Growth control of oral and superficial microorganisms using gallium compounds |
-
2004
- 2004-05-12 CN CNA2004100261065A patent/CN1695493A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2689783A2 (en) * | 2005-11-01 | 2014-01-29 | Mount Sinai School of Medicine of New York University | Growth control of oral and superficial microorganisms using gallium compounds |
| EP2689783A3 (en) * | 2005-11-01 | 2014-08-20 | Mount Sinai School of Medicine of New York University | Growth control of oral and superficial microorganisms using gallium compounds |
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