CN1680535A - Culturing system of external light biological reactor for plant tissue cutter - Google Patents
Culturing system of external light biological reactor for plant tissue cutter Download PDFInfo
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- CN1680535A CN1680535A CN 200510023286 CN200510023286A CN1680535A CN 1680535 A CN1680535 A CN 1680535A CN 200510023286 CN200510023286 CN 200510023286 CN 200510023286 A CN200510023286 A CN 200510023286A CN 1680535 A CN1680535 A CN 1680535A
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Abstract
An outlay plant tissue cutter optical bioreactor culture system which contains transfusion system, air transportation system, illumination system and bioreactor culture system, data processor, light-sensitive monitor, tee valve, incision system, air filter, vacuum/pressure pump. The silicone tube is the connection of the transfusion system and air transportation system to the bioreactor culture system; the outside of it is illumination system; and the other side is light-sensitive monitor. The invention can decrease the pollution of the incision greatly.
Description
Technical field
The present invention relates to a kind of culture systems, specifically is a kind of external light biological reactor for plant tissue cutter culture systems.Be used for plant tissue engineering field.
Background technology
The research of bioreactor starts from the large scale culturing of little algae forties in 20th century, developed rapidly after the eighties, developed miscellaneous bioreactor up to now, and can in bioreactor, insert pH value electrode, dissolved oxygen electrode, temperature electrode, cell density electrode, viscosity electrode etc., the signal that the sensor obtains can further be changed, realize the sequencing record and the control automatically of bioreactor.Land plant that is used to cultivate and large-scale seaweed cell or to organize mainly be forms such as callus, micropropagation body or gametophyte cell, its common feature is to have very strong growth, amplification ability, but continued growth after the cutting, and, individuality is constantly grown up in the culturing process, must smash just by regular cutting and can keep its undifferentiated state.The research of cultivating for the bioreactor of present plant tissue, in the culturing process when the tissue of cultivating and cell grow into certain volume and begin to influence its growth, because of present design does not all have at wire-electrode cutting device, cutting is smashed and is then wanted complete off-line operation as need, influence the experiment process, make troubles to experimental implementation, therefore, under the prerequisite that can not satisfy the demand on the hardware, the continuously long-term bioreactor culture apparatus system of online cutting can further realize the sequencing record and the control automatically of bioreactor in original design.
Find by prior art documents, people such as Yao-ming Huang are at " BiotechnologyProgress " 2002,18:62-71 has " the Dynamics of oxygen evolution and biomassproduction during cultivation of Agardhiella subulata microplantlrts in abubble-column photobioreactor under medium perfusion " that deliver and (utilizes airlift photobioreactor to cultivate and visit the research that formula new Asia algae micropropagation body carries out oxygen transferring power and biomass, " biotechnology progress ") in the literary composition, the size that proposes the suitable suspension culture of Agardhiella subulata micropropagation body is 3-10mm, must regularly cut in the micropropagation body culturing process and smash, in order to keep the high growth rates of micropropagation body, when the micropropagation bulk-growth surpasses 10mm, be cut to the suitable size of 3-5mm again and continue again to cultivate in the culturing process; The culturing system of light bioreactor of the Agardhiella subulata micropropagation body of this article proposition simultaneously is made up of gas transmission system, illumination system and reactor body three parts.Its weak point is: this system can only cultivate for the micropropagation body provides interim, can not realize the cultivation of continuously long-term bioreactor, in addition, this system can only carry out the off-line cutting to the micropropagation body, this off-line cutting mode has increased the microbial contamination rate of culturing process and loaded down with trivial details cutting work greatly, makes troubles to experiment; When being applied to large scale culturing, the off-line cutting then becomes conditioning step, has a strong impact on carrying out smoothly of experiment.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, a kind of external light biological reactor for plant tissue cutter culture systems is provided, achieve the online cutting in vegetable cell and the tissue culture procedures, the growth metabolism state that guarantees culture in the culturing process maintains the optimization point, greatly reduces the pollution rate of off-line cutting simultaneously.
The present invention is achieved by the following technical solutions, the present invention includes: transfusion system, gas delivery system, illumination system, bioreactor culture system, data receiving processor, on-line photosensitive monitor, three-way device, diced system, first air filter, vacuum/pressurized dual-purpose pump.Its mode of connection is: transfusion system is connected with bioreactor culture system by silicone tube; Gas delivery system links to each other with bioreactor culture system by silicone tube; Illumination system is fixed on the bioreactor culture system periphery; On-line photosensitive monitor is installed in culturing system of light bioreactor one side, transmits signals to data receiving processor; Three ends of three-way device connect two interfaces of bioreactor culture system and the interface of diced system respectively by silicone tube; Vacuum/pressurized dual-purpose pump, first air filter, diced system are connected in turn by the silicone tube head and the tail.
Transfusion system is made up of container for storing liquid and infusion pump.Container for storing liquid is connected with bioreactor culture system through infusion pump by silicone tube, and the fresh medium in the container for storing liquid is in infusion pump flows into bioreactor culture system.
Gas delivery system is by air pump, second air filter, and gas meter, the gas humidification device is formed.The gas humidification apparatus main body is a cylinder shape hemisphere top Glass Containers, and its top is integrated with gas inlet and pneumatic outlet, and the gas inlet stretches to apart from about 1/6 place of container bottom; Be connected in turn by the silicone tube head and the tail between the gas inlet of air pump, second air filter, gas meter and gas humidifying device; The pneumatic outlet of gas humidification device is connected with bioreactor culture system.
Bioreactor culture system is by gas distributor, whipping appts, dissolved oxygen electrode, pH electrode, stopple coupon, the air outlet, the 3rd air filter, fluid inlet, liquid outlet, first filters bolting silk, the cutting liquid return hole, and first liquid taking port, second filters bolting silk and second liquid taking port composition.Gas distributor is the hollow tube of being made by stainless material, form by ventpipe and gas distribution tube, ventpipe is inserted into the bioreactor culture system bottom position by the bioreactor culture system top, the gas distributor bottom is a gas distribution tube, be circular arc, be distributed in the periphery of bioreactor culture system rounded bottom surface, be integrated with circular production well above, ventpipe and gas distribution tube junction are the ventpipe contact; Whipping appts is made up of agitator arm and stirring rake, agitator arm by bioreactor culture system center of top position stretch into bioreactor culture system inside far from the bottom 1/3～1/4 place, agitator arm bottom is integrated with stirring rake; Stopple coupon stretches into bioreactor culture system inside far from about about 1/2 place, bottom; Dissolved oxygen electrode and pH electrode stretch under the liquid level of bioreactor culture system; The place, air outlet connects the 3rd air filter with silicone tube; The liquid outlet and the first liquid taking port place have double-deck 800 purposes first to filter the bolting silk and the second filtration bolting silk respectively.
Transfusion system inputs to bioreactor culture system with fresh medium by fluid inlet, and for keeping constant volume, excessive nutrient solution is flowed out automatically by the liquid outlet place in the bioreactor culture system, and the liquid outlet place has first to filter bolting silk; Gas delivery system links to each other with gas distributor by silicone tube, and gas distributor is inserted into the bioreactor culture system bottom position by the bioreactor culture system top, and for its cultivation provides competent gas, residual gas and waste gas are discharged through the air outlet; First liquid taking port and second liquid taking port are got liquid interface and second with first of three-way device respectively by silicone tube and are got liquid interface and link to each other, and the first liquid taking port place has second to filter bolting silk; The cutting liquid return hole is connected with diced system by silicone tube; Dissolved oxygen electrode and pH electrode stretch into bioreactor culture system inside by the bioreactor culture system top.
Three-way device one end takes the shape of the letter U, and an end is an in-line, is made by the high-boron-silicon glass material.2 ports of U end are: U end first is got liquid interface and is got liquid interface with U end second and link to each other with second liquid taking port with first liquid taking port of bioreactor culture system by silicone tube respectively, and in the silicone tube junction first tongs and second tongs is installed; One word end links to each other with diced system by silicone tube for the cutting fluid inlet.
When bioreactor culture system is in cultivation stage, first tongs and the second tongs Close All, nutrient solution can not enter diced system; When needing cutting, open second tongs at the second liquid taking port place that does not have bolting silk, the nutrient solution culture enters diced system simultaneously; When cutting need to finish the flushing diced system, opening installation had second to filter first tongs at the first liquid taking port place of bolting silk, and nutrient solution enters diced system, and culture then is trapped in the bioreactor culture system.
Diced system is by cutting jar, cutting unit, cutting parameter setting panel, cutting feed liquor interface, cutting liquid outlet and PNPV mouth composition.Its mode of connection is: cutting parameter is set the bottom that panel is positioned at the cutting jar, and cutting jar and cutting parameter are set between the panel and can be dismantled, and are convenient to cleaning and sterilizing; Cutting unit is the cross knife-edge, and two ends are inclined upwardly and erect, and is positioned at cutting parameter and sets the join middle position of one side of panel and cutting jar, can freely dismantle, and revolution that cutting unit turns round and clipping time are preestablished according to requirement of experiment by cutting parameter setting panel; The cutting upper end is provided with the PNPV mouth, link to each other with vacuum/pressurized dual-purpose pump with first air filter by silicone tube, when needs Cutting Control jar feed liquor, open the vacuum switch of vacuum/pressurized dual-purpose pump, nutrient solution passes through three-way device, cutting feed liquor interface through cutting jar upper position is sucked in the cutting jar, otherwise, in the time will cutting liquid and be back in the bioreactor culture system, then open the pressure switch of vacuum/pressurized dual-purpose pump, cutting liquid is press-fit in the bioreactor culture system through the cutting liquid outlet of cutting jar bottom.
When the present invention works, fresh medium in the container for storing liquid is in infusion pump flows into bioreactor culture system, to keep the long-term cultured continuously of bioreactor system, constant for the volume of culture that keeps bioreactor culture system, excessive nutrient solution is flowed out automatically by the liquid outlet place, the gas that air pump provides is through the second air filter filtration sterilization, behind the gas humidification device humidification, provide competent gas in the bioreactor culture system bottom for its cultivation by gas distributor, remaining gas and waste gas are discharged by the air outlet, the place, air outlet connects the 3rd air filter, to guarantee sterile state in the reactor, illumination system provides necessary light intensity for bioreactor culture system, bioreactor culture system enters cultivation stage, on-line photosensitive monitor is determined the size of purpose culture by photosensor signal, continuously, on-line monitoring purpose culture size and concentration, and with signal feedback to data receiving processor, the size that shows the purpose culture when data receiving processor reaches the setting size parameter, when simultaneously quantity reaches preset proportion and counts, the prompting cutoff signal appears, at this moment, open second tongs of three-way device, open the vacuum switch of vacuum/pressurized dual-purpose pump, culture in the bioreactor culture system and nutrient solution enter in the cutting jar jointly, after reaching cutting jar setting volume, close vacuum switch earlier, close second tongs again, set revolution and the clipping time that panel is set the cutting unit running as requested by cutting parameter, open the cutting switch, the cutting beginning; After cutting finishes, the pressure switch of opening vacuum/pressurized dual-purpose pump, cutting liquid is back in the bioreactor culture system by cutting liquid outlet, silicone tube, cutting liquid return hole by the pressure effect, closes the pressure switch of vacuum/pressurized dual-purpose pump; Then, open first tongs of three-way device, open the vacuum switch of vacuum/pressurized dual-purpose pump, nutrient solution in the bioreactor culture system enters in the cutting jar, culture is then filtered bolting silk by second of the first liquid taking port place and is trapped in the bioreactor culture system, when the nutrient solution volume that enters the cutting jar reaches the setting volume, close vacuum switch earlier, close first tongs again, set panel by cutting parameter and set the flushing parameter, open the cutting switch, flushing cutting jar, after flushing finishes, the pressure switch of opening vacuum/pressurized dual-purpose pump, cutting liquid by the pressure effect by the cutting liquid outlet, silicone tube, the cutting liquid return hole is back to bioreactor culture system, close the pressure switch of vacuum/pressurized dual-purpose pump, this operation repeats 2-3 time, and cutting process finishes, and bioreactor culture system enters cultivation stage.
System of the present invention is a closed system; reduced microbiological contamination; solved simultaneously in vegetable cell and the tissue culture procedures because of the individual excessive practical problems of bringing to experiment of purpose culture; guaranteed that the growth metabolism state of culture maintains the optimization point in the culturing process; realized the continuously long-term bioreactor cultivation of the online cutting of plant tissue, for the possible approach that provides is provided in mass-producing.
Compared with prior art, the present invention is used cutting unit and bioreactor, realized the online cutting in vegetable cell and the tissue culture procedures, greatly reduce the pollution rate of off-line cutting, improved working efficiency, guaranteed that the quality and the growth metabolism state thereof of purpose culture maintains the optimization point in the culturing process; Cutting unit among the present invention has independently cutting parameter setting panel, and is workable, can set different cutting parameters according to different purpose cultures; Replace traditional visual method by on-line photosensitive monitor testing goal culture size, improved science, the accuracy of experiment; Be widely used in dissimilar bioreactors, cutting unit is easy to disassembly, cleaning, be convenient to independent operation, two big systems (bioreactor culture system and diced system) of total system are not only independent separately but also get in touch each other, avoid in the cutting process cross influence with other culture parameters of bioreactor (as ventilation, stirring etc.), improved the accuracy of experiment single parameter research.
Description of drawings
Fig. 1 is a structural representation of the present invention
Fig. 2 is a transfusion system structural representation of the present invention
Fig. 3 is a gas delivery system structural representation of the present invention
Fig. 4 is a bioreactor culture system structural representation of the present invention
Fig. 5 is a gas distributor structure front view of the present invention
Fig. 6 is a gas distributor structure vertical view of the present invention
Fig. 7 is a three-way device structural representation of the present invention
Fig. 8 is a diced system structural representation of the present invention
,1. 2. 3. 4. 5. 6. 7. 8. 9. 10./ 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22.pH 23.24. 25. 26. 27. 28.29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42.43. 44. 45. 46. 47. 48. 49.
Embodiment
Shown in Fig. 1-8, the present invention includes: transfusion system 1, gas delivery system 2, illumination system 3, bioreactor culture system 4, data receiving processor 5, on-line photosensitive monitor 6, three-way device 7, diced system 8, the first air filters 9, vacuum/pressurized dual-purpose pump 10.Its mode of connection is: transfusion system 1 is connected with bioreactor culture system 4 by silicone tube; Gas delivery system 2 is connected with bioreactor culture system 4 by silicone tube; Illumination system 3 is fixed on bioreactor culture system 4 peripheries; On-line photosensitive monitor 6 is installed in culturing system of light bioreactor 4 one sides, transmits signals to data receiving processor 5; Three ends of three-way device 7 connect two interfaces of bioreactor culture system 4 and the interface of diced system 8 respectively by silicone tube; Vacuum/pressurized dual-purpose pump 10, first air filter 9, diced system 8 are connected in turn by the silicone tube head and the tail.
Transfusion system 1 is made up of container for storing liquid 11 and infusion pump 12.Container for storing liquid 11 is connected with bioreactor culture system 4 through infusion pump 12 by silicone tube, and the fresh medium in the container for storing liquid 11 flows in the bioreactor culture system 4 through infusion pump 12.
Gas delivery system 2 is by air pump 13, the second air filters 14, gas meter 15, and gas humidification device 16 is formed.The top of gas humidification device 16 is integrated with gas inlet 17 and pneumatic outlet 18, and gas inlet 17 stretches to apart from about 1/6 place of container bottom; Connect in turn by the silicone tube head and the tail between the gas inlet 17 of air pump 13, second air filter 14, gas meter 15 and gas humidifying device 16; The pneumatic outlet 18 of gas humidification device 16 is connected with bioreactor culture system 4.
Bioreactor culture system 4 is by fluid inlet 19, gas distributor 20, dissolved oxygen electrode 21, pH electrode 22, whipping appts 23, cutting liquid return hole 24, stopple coupon 25, air outlet 26, the 3rd air filter 27, liquid outlet 28, the first filters bolting silk 29, first liquid taking port 30, the second filters bolting silk 31 and second liquid taking port 32 is formed.Gas distributor 20 is hollow tubes of being made by stainless material, form by ventpipe 35 and gas distribution tube 36, ventpipe 35 is inserted into bioreactor culture system 4 bottom positions by bioreactor culture system 4 tops, gas distributor 20 bottoms are gas distribution tube 36, be circular arc, be distributed in the periphery of bioreactor culture system 4 rounded bottom surfaces, be integrated with circular production well 38 above, ventpipe 35 and gas distribution tube 36 junctions are ventpipe contact 37; Whipping appts 23 is made up of agitator arm 33 and stirring rake 34, agitator arm 33 by bioreactor culture system 4 center of top positions stretch into bioreactor culture system 4 inside far from the bottom 1/3～1/4 place, agitator arm 33 bottoms are integrated with stirring rake 34; Stopple coupon 25 stretch into bioreactor culture system 4 inside far from the bottom about 1/2 place; Dissolved oxygen electrode 21 and pH electrode 22 stretch under the liquid level of bioreactor culture system 4; 26 places, air outlet connect the 3rd air filter 27 with silicone tube; Transfusion system 1 inputs to bioreactor culture system 4 with fresh medium by fluid inlet 19, excessive nutrient solution is flowed out automatically by liquid outlet 28 places in the bioreactor culture system 4, liquid outlet 28 and first liquid taking port, 30 places have double-deck 800 purposes first to filter bolting silk 29 respectively and the second filtration bolting silk, 31, the first liquid taking ports 30 link to each other with three-way device 7 by silicone tube with second liquid taking port 32; Cutting liquid return hole 24 is connected with diced system 8 by silicone tube.
Three-way device 7 one ends take the shape of the letter U, and an end is an in-line, is made by the high-boron-silicon glass material.2 ports of U end are: U end first is got liquid interface 39 and is got liquid interface 40 with U end second and link to each other with second liquid taking port 32 with first liquid taking port 30 of bioreactor culture system 4 by silicone tube respectively, and in the silicone tube junction first tongs 41 and second tongs 42 is installed; One word end links to each other with diced system 8 by silicone tube for cutting fluid inlet 43.
Diced system 8 is by cutting jar 46, cutting unit 48, cutting parameter setting panel 49, cutting feed liquor interface 44, cutting liquid outlet 47 and PNPV mouth 45 compositions.Its mode of connection is: cutting parameter is set the bottom that panel 49 is positioned at cutting jar 46, cutting jar 46 and cutting parameter are set between the panel 49 and can be dismantled, cutting unit 48 is the cross knife-edge, two ends are inclined upwardly and erect, be positioned at cutting parameter and set the join middle position of one side of panel 49 and cutting jars 46, cutting jar 46 tops are provided with PNPV mouth 45, link to each other with vacuum/pressurized dual-purpose pump 10 with first air filter 9 by silicone tube.
Claims (8)
1, a kind of external light biological reactor for plant tissue cutter culture systems, comprise: transfusion system (1), gas delivery system (2), illumination system (3), bioreactor culture system (4), it is characterized in that, also comprise: data receiving processor (5), on-line photosensitive monitor (6), three-way device (7), diced system (8), first air filter (9), vacuum/pressurized dual-purpose pump (10), its mode of connection is: transfusion system (1) is connected with bioreactor culture system (4) by silicone tube, gas delivery system (2) is connected with bioreactor culture system (4) by silicone tube, illumination system (3) is fixed on bioreactor culture system (4) periphery, on-line photosensitive monitor (6) is located at culturing system of light bioreactor (4) one sides, be connected with data receiving processor (5), three ends of three-way device (7) connect two interfaces of bioreactor culture system (4) and an interface of diced system (8), vacuum/pressurized dual-purpose pump (10) respectively by silicone tube, first air filter (9), diced system (8) is connected in turn by the silicone tube head and the tail.
2, external light biological reactor for plant tissue cutter culture systems according to claim 1, it is characterized in that, transfusion system (1) is made up of container for storing liquid (11) and infusion pump (12), and container for storing liquid (11) is connected with bioreactor culture system (4) through infusion pump (12) by silicone tube.
3, external light biological reactor for plant tissue cutter culture systems according to claim 1, it is characterized in that, gas delivery system (2) is by air pump (13), second air filter (14), gas meter (15), gas humidification device (16) is formed, the top of gas humidification device (16) is integrated with gas inlet (17) and pneumatic outlet (18), gas inlet (17) stretches to apart from container bottom 1/6 place, air pump (13), second air filter (14), connected in turn by the silicone tube head and the tail between gas meter (15) and gas inlet (17), the pneumatic outlet (18) of gas humidification device (16) is connected with bioreactor culture system (4).
4, external light biological reactor for plant tissue cutter culture systems according to claim 1, it is characterized in that, bioreactor culture system (4) is by fluid inlet (19), gas distributor (20), dissolved oxygen electrode (21), pH electrode (22), whipping appts (23), cutting liquid return hole (24), stopple coupon (25), air outlet (26), the 3rd air filter (27), liquid outlet (28), first filters bolting silk (29), first liquid taking port (30), second filters bolting silk (31) and second liquid taking port (32) composition, gas distributor (20) is distributed in the periphery of bioreactor culture system (4) rounded bottom surface, whipping appts (23) is made up of agitator arm (33) and stirring rake (34), agitator arm (33) by bioreactor culture system (4) center of top position stretch into bioreactor culture system (4) inner apart from the bottom 1/3～1/4 place, agitator arm (33) bottom is integrated with stirring rake (34), stopple coupon (25) stretch into bioreactor culture system (4) inner apart from the bottom 1/2 place, dissolved oxygen electrode (21) and pH electrode (22) stretch under the liquid level of bioreactor culture system (4), air outlet (26) is located to connect the 3rd air filter (27) with silicone tube, transfusion system (1) inputs to bioreactor culture system (4) with fresh medium by fluid inlet (19), excessive nutrient solution is located automatic outflow by liquid outlet (28) in the bioreactor culture system (4), liquid outlet (28) and first liquid taking port (30) locate to have respectively first to filter the bolting silk (29) and the second filtration bolting silk (31), first, second liquid taking port (30,32) link to each other with three-way device (7) by silicone tube, cutting liquid return hole (24) is connected with diced system (8) by silicone tube.
5, external light biological reactor for plant tissue cutter culture systems according to claim 4, it is characterized in that, gas distributor (20) is the hollow tube of being made by stainless material, form by ventpipe (35) and gas distribution tube (36), ventpipe (35) links to each other with the pneumatic outlet (18) of gas humidification device (16), be inserted into bioreactor culture system (4) bottom position by bioreactor culture system (4) top, gas distributor (20) bottom is gas distribution tube (36), be circular arc, be distributed in the periphery of bioreactor culture system (4) rounded bottom surface, be integrated with circular production well (38) above, ventpipe (35) and gas distribution tube (36) junction are ventpipe contact (37).
6, external light biological reactor for plant tissue cutter culture systems according to claim 1, it is characterized in that, three-way device (7) one ends take the shape of the letter U, one end is an in-line, two ports of U end are: U end first is got liquid interface (39) and U and is held second to get liquid interface (40) and link to each other with second liquid taking port (32) with first liquid taking port (30) of bioreactor culture system (4) by silicone tube respectively, and be provided with first tongs (41) and second tongs (42) in the silicone tube junction, one word end links to each other with diced system (8) by silicone tube for cutting fluid inlet (43).
7, external light biological reactor for plant tissue cutter culture systems according to claim 1, it is characterized in that, diced system (8) is by cutting jar (46), cutting unit (48), cutting parameter is set panel (49), cutting feed liquor interface (44), cutting liquid outlet (47) and PNPV mouth (45) are formed, cutting parameter is set the bottom that panel (49) is positioned at cutting jar (44), cutting unit (48) is positioned at cutting parameter and sets the join middle position of one side of panel (49) and cutting jar (46), cutting jar (a 46) top is provided with PNPV mouth (45), links to each other with vacuum/pressurized dual-purpose pump (10) with first air filter (9) by silicone tube.
8, external light biological reactor for plant tissue cutter culture systems according to claim 7 is characterized in that, cutting unit (48) is the cross knife-edge, and two ends are inclined upwardly and erect, and cutting jar (44) and cutting parameter are set between the panel (47) and can be dismantled.
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| CNB2005100232866A CN1303200C (en) | 2005-01-13 | 2005-01-13 | Culturing system of external light biological reactor for plant tissue cutter |
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| CNB2005100232866A CN1303200C (en) | 2005-01-13 | 2005-01-13 | Culturing system of external light biological reactor for plant tissue cutter |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011026964A3 (en) * | 2009-09-07 | 2011-12-22 | Romil Beheer Bv | Method and apparatus for treating fermentable substances |
| CN102978113A (en) * | 2011-09-06 | 2013-03-20 | 江苏省农业科学院 | Stirring, aerating and cell filtering bioreactor integrating |
| KR101522182B1 (en) * | 2015-02-10 | 2015-05-22 | 주식회사 에이이 | The Culture Method of High Efficiency Spirulina Using Dispersion Method |
| CN105352999A (en) * | 2015-10-12 | 2016-02-24 | 临沂大学 | Ex-situ determination method for pH value of plant sap |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1201826A (en) * | 1997-06-05 | 1998-12-16 | 华南师范大学 | Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method |
| CN1232631C (en) * | 2004-03-04 | 2005-12-21 | 江南大学 | Coupling biologic reactor |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011026964A3 (en) * | 2009-09-07 | 2011-12-22 | Romil Beheer Bv | Method and apparatus for treating fermentable substances |
| CN102978113A (en) * | 2011-09-06 | 2013-03-20 | 江苏省农业科学院 | Stirring, aerating and cell filtering bioreactor integrating |
| CN102978113B (en) * | 2011-09-06 | 2014-06-11 | 江苏省农业科学院 | Stirring, aerating and cell filtering bioreactor integrating |
| KR101522182B1 (en) * | 2015-02-10 | 2015-05-22 | 주식회사 에이이 | The Culture Method of High Efficiency Spirulina Using Dispersion Method |
| CN105352999A (en) * | 2015-10-12 | 2016-02-24 | 临沂大学 | Ex-situ determination method for pH value of plant sap |
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