CN105352999A - Ex-situ determination method for pH value of plant sap - Google Patents
Ex-situ determination method for pH value of plant sap Download PDFInfo
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- CN105352999A CN105352999A CN201510656582.3A CN201510656582A CN105352999A CN 105352999 A CN105352999 A CN 105352999A CN 201510656582 A CN201510656582 A CN 201510656582A CN 105352999 A CN105352999 A CN 105352999A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The invention discloses an ex-situ determination method for the pH value of plant sap. The ex-situ determination method comprises the following steps: washing an air-dried or kiln-dried plant organ sample with tap water and deionized water five times respectively, then drying the plant organ sample in a drying box at 40 DEG C, then crushing the plant organ sample with pulverizer and carrying out screening with a 60-mesh sieve; accurately weighing 0.4 to 0.6 g of powder obtained in the previous step, placing the powder in a teflon crucible, then adding 2 to 5 mL of deionized water to allow the powder to be totally immersed in the deionized water and then carrying out standing and immersion for 30 to 60 min; and filtering out leachate and determining the pH value of the leachate by using a pH electrode so as to obtain the pH value of plant sap. The method provided by the invention overcomes the technical problem that the pH value of plant sap can only be determined in situ in an organ and provides technical guarantee for application of the pH value to research of fields like phytophysiology, plant nutriology and biogeochemistry.
Description
Technical field
The present invention relates to the outer method for measuring of a Plants Different Organs body fluid pH value device.
Background technology
In recent years, along with the improvement and bring new ideas of analytical technology, the method measuring pH value is constantly weeded out the old and bring forth the new, comprising acid base indicator method, weak acid flux method, compound pH electrode method, fluorescent spectrometry, Confocal laser scanning microscopy, microelectrode pH sensor method based on agar or glass dust.The foundation of these methods is all that the mensuration of plant sap pH value provides good technical foundation.At present, the mensuration of plant sap pH value generally adopts compound pH microelectrode to carry out in-site detecting, but to some thinner or tiny organ samples as leaf, stem etc., be difficult to realize in-site detecting, and some wooden hard plants easily cause testing tool to be lost (tip as pH microelectrode is lost), therefore set up a kind of simple and easy, the outer method for measuring of plant sap pH value device can be realized extremely urgent, plant organ body fluid pH value can be made as the effective indicating means of one or substitute parameter, be applied to plant physiology, the applied research in Plant Nutrition or biomass geochemistry field.
Summary of the invention
In order to overcome above-mentioned existing methodical deficiency, the present invention is directed to existing plant sap pH value can only the shortcoming of in-site detecting, proposes the outer method for measuring of a kind of plant sap pH value device that is very simple and easy, that can realize.
The outer method for measuring of a kind of plant sap pH value device in the present invention, carry out according to following steps:
(1) successively with tap water with deionized water cyclic washing is air-dry or each 5 times of the plant organ sample of drying, dry at 40 DEG C in drying box, then pulverize with plant comminutor, cross 60 order analyses sieves;
(2) precise 0.4-0.6g step (1) gained powder, is placed in teflon crucible, then adds 2-5mL deionized water, makes the complete submergence powder of deionized water, leaves standstill and soaks 30-60min;
(3) leachate of step (2) is leached, by the pH value of pH determination of electrode leachate, namely obtain the pH value of plant sap.
Further, in step (2), the amount of powder used is 0.5g, and the volume adding deionized water is 2mL, and soak time is 30min.
Further, in step (3), pH electrode used is compound pH microelectrode.
compared with prior art, tool has the following advantages in the present invention:
1. the device achieving plant sap pH value measures outward, and easy to operate
Soak the powdered sample of plant Different Organs with deionized water, plant sap is leached completely, and the device achieving plant organ body fluid pH value measures outward, compared to in-site detecting, easy to operate.
2. electrode easy cleaning, more durable
Each extraorgan, leachate is measured, compared to in-site detecting, pH electrode easy cleaning, more durable.
3. accuracy is high, and testing result is reliable
By the optimization to immersion liquid sum soak time, find that these factors can be ignored substantially on the impact that plant sap pH value measures, the change of pH value is in the error range measuring permission, and therefore assay method accuracy of the present invention is high, reliable results.
Assay method of the present invention is easy to operate, instrument is durable, measurement result is accurate, and be suitable for the mensuration of Different Organs body fluid pH value of terrestrial plant, hydrophyte and sea-plant, range of application is comparatively wide, and practicality is comparatively strong, has higher operability.
Accompanying drawing explanation
Fig. 1 is the impact that soak solution (deionized water) volume measures pH value;
Fig. 2 is the impact that soak time measures pH value.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
If do not specialize, the convenient source that raw material used in embodiment and instrument are well known to those skilled in the art and instrument.
The fruit sample that the present embodiment uses is commercially available apple, shaddock, pears and orange.The other plant sample that the present embodiment uses is the root of the Red Weigela Florida A.DC gathered voluntarily, stem and leaf, the root of Bupleurum scorzonerifoliumWild, stem and leaf, the flower of cynomorium songaricum and stem, the stem of flower anchor.
The present embodiment adopts HI9075 type pH meter (Italian HANNA company), and supporting pH electrode adopts compound pH microelectrode (MI-411 type, Bedford company of the U.S.).
Embodiment 1
(1) successively with tap water with deionized water cyclic washing is air-dry or each 5 times of the plant organ sample of drying, dry at 40 DEG C in air dry oven, then pulverize with plant comminutor, cross 60 order analyses sieves.
(2) investigation of soak solution volume: the plant sample powder of precise 0.5g step (1), be placed in teflon crucible, add 2 respectively to it, 3,4,5mL deionized water, make the complete submergence sample powder of deionized water, leave standstill and soak 30min, leached by leachate after soaking, adopt compound pH microelectrode to measure leachate, the change curve of pH value as shown in Figure 1.Result shows, the mensuration of deionized water volume to pH value has no significant effect.
(3) investigation of soak time: the plant sample powder of precise 0.5g step (1), be placed in teflon crucible, 2mL deionized water is added to it, make the complete submergence sample powder of deionized water, leave standstill and soak 10-360min, leached by leachate after soaking, adopt compound pH microelectrode to measure leachate, the change curve of pH value as shown in Figure 2.Result shows, soak after 30 minutes, pH value tends towards stability.
(4) the plant sample powder of precise 0.5g step (1), is placed in teflon crucible, adds 2mL deionized water to it, makes the complete submergence sample powder of deionized water, leaves standstill and soaks 30min.
(5) by soak after leachate leach, with compound pH microelectrode, leachate is measured, measurement result and standard deviation as shown in table 1.
Contrast experiment: directly compound pH microelectrode is inserted the organ samples such as apple, shaddock, pears and orange and carry out in-site detecting, measurement result and standard deviation list in table 2.
The body fluid pH value (n=6) of the different plant organs that table 1 the inventive method measures
The body fluid pH value (n=5) of 4 Plants organ samples of table 2 in-site detecting
As can be seen from above experimental result, the inventive method measurement result is reliable and stable, practicality is comparatively strong, is with a wide range of applications.
The explanation of above embodiment just understands method of the present invention and core concept thereof for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (3)
1. the outer method for measuring of plant sap pH value device, carry out according to following steps:
(1) successively with tap water with deionized water cyclic washing is air-dry or each 5 times of the plant organ sample of drying, dry at 40 DEG C in drying box, then pulverize with plant comminutor, cross 60 order analyses sieves;
(2) precise 0.4-0.6g step (1) gained powder, is placed in teflon crucible, then adds 2-5mL deionized water, makes the complete submergence powder of deionized water, leaves standstill and soaks 30-60min;
(3) leachate of step (2) is leached, by the pH value of pH determination of electrode leachate, namely obtain the pH value of plant sap.
2. the outer method for measuring of plant sap pH value device as claimed in claim 1, it is characterized by: in step (2), the amount of powder used is 0.5g, and the volume adding deionized water is 2mL, and soak time is 30min.
3. the outer method for measuring of plant sap pH value device as claimed in claim 1, it is characterized by: in step (3), pH electrode used is compound pH microelectrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510656582.3A CN105352999B (en) | 2015-10-12 | 2015-10-12 | A kind of outer method for measuring of plant sap pH value device |
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| CN201510656582.3A CN105352999B (en) | 2015-10-12 | 2015-10-12 | A kind of outer method for measuring of plant sap pH value device |
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| CN105352999A true CN105352999A (en) | 2016-02-24 |
| CN105352999B CN105352999B (en) | 2018-02-16 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1473195A (en) * | 2000-10-30 | 2004-02-04 | �ֲ����ѧ�������˾ | Identification and characterization of anthocyanin mutant (ANT1) in tomato |
| CN1680535A (en) * | 2005-01-13 | 2005-10-12 | 上海交通大学 | Culturing system of external light biological reactor for plant tissue cutter |
| CN101336880A (en) * | 2008-08-07 | 2009-01-07 | 北京昂立达技术有限责任公司 | Art pumpkin leaves extract and use in cosmetics |
| CN103651265A (en) * | 2013-11-20 | 2014-03-26 | 中国科学院南海海洋研究所 | Method for detecting coral larva calcification rate by means of microelectrodes |
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2015
- 2015-10-12 CN CN201510656582.3A patent/CN105352999B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1473195A (en) * | 2000-10-30 | 2004-02-04 | �ֲ����ѧ�������˾ | Identification and characterization of anthocyanin mutant (ANT1) in tomato |
| CN1680535A (en) * | 2005-01-13 | 2005-10-12 | 上海交通大学 | Culturing system of external light biological reactor for plant tissue cutter |
| CN101336880A (en) * | 2008-08-07 | 2009-01-07 | 北京昂立达技术有限责任公司 | Art pumpkin leaves extract and use in cosmetics |
| CN103651265A (en) * | 2013-11-20 | 2014-03-26 | 中国科学院南海海洋研究所 | Method for detecting coral larva calcification rate by means of microelectrodes |
Non-Patent Citations (2)
| Title |
|---|
| 刘丽娟等: "植物粗提物对淡色库蚊幼虫的生物活性", 《公共卫生与预防医学》 * |
| 施倩倩: "构树与桑树叶片的碳酸酐酶胞外酶活力比较", 《安徽农业科学》 * |
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