CN1672520A - Pesticidal disease-preventing plant growth promoter and its prepn - Google Patents
Pesticidal disease-preventing plant growth promoter and its prepn Download PDFInfo
- Publication number
- CN1672520A CN1672520A CNA200410022134XA CN200410022134A CN1672520A CN 1672520 A CN1672520 A CN 1672520A CN A200410022134X A CNA200410022134X A CN A200410022134XA CN 200410022134 A CN200410022134 A CN 200410022134A CN 1672520 A CN1672520 A CN 1672520A
- Authority
- CN
- China
- Prior art keywords
- growth promoter
- disease
- plant insecticide
- prevention growth
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The pesticidal disease-preventing plant growth promoter contains berberine hydrochloride 0.06-0.36 wt%, matrine 0.01-0.06 wt%, total flavone 0.23-1.38 wt%, and is prepared into solution, paste or powder. The preparation process of the pesticidal disease-preventing plant growth promoter includes mixing raw material medicines including phellodendron bark in 1 weight portions, tobacco in 0.25-3.5 weight portions, flavescent sophora root in 0.25-4 weight portions and chinaberry tree bark in 0.15-2 weight portions; twice or thrice decoction in water for 45-70 min each, collecting volatile oil, filtering medicine liquid, and concentration to relative density 1.05-1.3. The present invention has relatively high preventing and treating effect on several kinds of pests and diseases and has no toxicity and no residue, and may be used in production of green vegetable and fruits.
Description
Affiliated technical field: the invention belongs to pesticide field, specifically is a kind of plant insecticide disease-prevention growth promoter and preparation method thereof.
Background technology: along with the development of society, the arrival of industrial civilization has brought impayable material wealth to the mankind.Yet, the unification of man and nature, coordination, the ecological balance have been suffered great destruction under the situation that with people's will is not transfer, human owing to too much eaten food such as the vegetables of having used chemical pesticide, fruit, persticide residue because of chemical pesticide, the mankind have been brought a series of disease, as developed as one pleases and to cause serious consequence to the mankind.
Summary of the invention:
The purpose of this invention is to provide a kind of plant insecticide disease-prevention growth promoter and preparation method thereof, the plant insecticide disease-prevention growth promoter has the better prevention effect to multiple damage by disease and insect, has the advantage of nontoxic residue-free, can be used for pollution-free vegetable, fruit production, its preparation method technology is simple, and is with low cost.
The objective of the invention is to realize by following technical proposals:
Plant insecticide disease-prevention growth promoter of the present invention is liquor or paste or the pulvis that contains Berberine hydrochloride 0.06～0.36%, matrine 0.01～0.06%, general flavone 0.23～1.38%.
The preparation method of plant insecticide disease-prevention growth promoter of the present invention, be by weight primary crude drug to be mixed, main primary crude drug is 1 part of golden cypress, 0.25～3.5 part on tobacco leaf, 0.25～4 part of kuh-seng, 0.15～2 part of azedarach, the water that per 1 part of primary crude drug adding is 8～12 parts decocts 2～3 times, each 45～70 minutes, collect simultaneously volatile oil and soup, with medical filtration, be concentrated into relative density 1.05～1.3, obtain plant insecticide disease-prevention growth promoter liquor.
In the such scheme, also can add 0.15～2 part of sweet wormwood in the primary crude drug.
In the such scheme, also can add 0.15～1.5 part of mother chrysanthemum in the primary crude drug.
In the such scheme, the plant insecticide disease-prevention growth promoter liquor that obtains adds 0.2～0.4% aromatic, 2～4% preservatives, 3～6% synergist in proportion and promptly gets plant insecticide disease-prevention growth promoter paste.
In the such scheme, the plant insecticide disease-prevention growth promoter liquor that obtains is spray dried to extract powder with spray drying tower, adds 8% sodium bicarbonate, 10% fumaric acid fine powder, mixes promptly to get plant insecticide disease-prevention growth promoter pulvis.
The present invention utilizes Berberine hydrochloride and the matrine that extracts in the primary crude drug, and general flavone administers the damage by disease and insect of plant, and is promoted the growth of plant by the general flavone that extracts.
The present invention changes chemical pesticide by using plant pesticide, and reduces human because of the damage of agricultural chemicals residual in the vegetables of having taken the use chemical pesticide, the fruit to health.Plant insecticide disease-prevention growth promoter of the present invention be primarily aimed at vegetables, fruit tree fungi, virus, aphid control and promote plant growing.
Therefore, plant insecticide disease-prevention growth promoter of the present invention has the better prevention effect to multiple damage by disease and insect, has the advantage of nontoxic residue-free, can be used for pollution-free vegetable, fruit production; Its preparation method technology is simple, and is with low cost.
Embodiment:
The invention is further illustrated by the following examples, and the present invention is not limited only to described embodiment.
Embodiment one
Get golden cypress 30kg, tobacco leaf 25kg, kuh-seng 30kg, azedarach 15kg decocts secondary with above-mentioned primary crude drug mixture, adds the drinking water of 1200L at every turn, each 45 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.05～1.08, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.15%, matrine 0.02%, general flavone 0.43%.Add 0.2% aromatic, 2% preservative, the clear paste of the formulated plant insecticide disease-prevention growth promoter of 3% synergist again.
Embodiment two
Get golden cypress 60kg, tobacco leaf 15kg, kuh-seng 15kg, azedarach 10kg, above-mentioned primary crude drug mixture is decocted secondary, boil after for the first time soaking 25min, add the drinking water of 800L at every turn, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.20～1.30, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.06%, matrine 0.01%, general flavone 0.23%.Add 0.4% aromatic, 4% preservative, the agent of the formulated plant insecticide disease-prevention growth promoter of 6% synergist thick paste again.
Embodiment three
Get golden cypress 7.2kg, tobacco leaf 25kg, kuh-seng 28.5kg, azedarach 14.4kg, sweet wormwood 14.4kg, mother chrysanthemum 10.5kg decocts above-mentioned primary crude drug mixture three times, boils after for the first time soaking 40min, each drinking water that adds 800L, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature are concentrated into relative density 1.10, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.36%, matrine 0.06%, general flavone 1.38%, be spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
Embodiment four
Get golden cypress 30kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 25.5kg, sweet wormwood 4.5kg decocts secondary with above-mentioned primary crude drug mixture, boils after for the first time soaking 30min, adds the drinking water of 1000L at every turn, each 60 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.05～1.08, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.16%, matrine 0.02%, general flavone 0.82%.Add 0.3% aromatic, 3% preservative, the clear paste of the formulated plant insecticide disease-prevention growth promoter of 4% synergist again.
Embodiment five
Get golden cypress 30kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 25.5kg, mother chrysanthemum 4.5kg decocts secondary with above-mentioned primary crude drug mixture, boils after for the first time soaking 30min, adds the drinking water of 800L at every turn, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.20～1.30, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.16%, matrine 0.02%, general flavone 0.83%.Add 0.3% aromatic, 3% preservative, the agent of the formulated plant insecticide disease-prevention growth promoter of 5% synergist thick paste again.
Embodiment six
Get golden cypress 15kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 15kg, sweet wormwood 15kg, mother chrysanthemum 15kg decocts above-mentioned primary crude drug mixture three times, boils after for the first time soaking 30min, each drinking water that adds 800L, each 60 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature are concentrated into relative density 1.10, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.26%, matrine 0.05%, general flavone 1.03%, be spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
The plant insecticide disease-prevention growth promoter (code name is natural plant protection) of the present invention's preparation is as follows in the field control effectiveness test result who prevents and treats disease:
One, prevents and treats anthracnose of grape, anthracnose
1, experiment purpose
" natural plant protection " is a kind of novel plant source biological pesticide, and anthracnose of grape, anthracnose are two big main diseases of northern China viny region, and extensively cause harm grape young sprout, blade, fruit are the key factors that causes the grape underproduction and reduce quality." natural plant protection " used in this experiment in the grape growth phase, then contrast as medicament with conventional sterilization agent methyl Tobe simultaneously, with checking " natural plant protection " control efficiency to anthracnose of grape, anthracnose.
2, experimental condition
2.1 trial crops
Seeds: grape variety: auspicious Bill (be commonly called as: American Black is carried)
The age of tree: life in 2 years
2.2 controlling object
Anthrachose of grape, anthracnose
3, test method
3.1 medicament
3.1.1 test medicine
" natural plant protection " botanical biological pesticide stoste is provided by Chengdu En Wei group, establishes totally 4 the concentration processing of 30 times, 50 times, 100 times, 200 times liquid.
3.1.2 contrast medicament
The methyl Tobe is the compound wetting powder of 60% ultra micro then
3.1.3 blank
Clear water is a blank
3.2 sub-district design
5 strains of every sub-district, area 10m
2, district's group is arranged at random, repeats 4 times.
3.3 dispenser period and method
For the first time spraying time May 23,, in June 3, each spray on June 13 once, spray medicine altogether 3 times, spray medicine at every turn and all before at 12 o'clock in the morning, finished later on according to weather conditions.The spraying machine tool adopts conventional knapsack hand sprayer, and every strain even spraying is moistening to the blade face positive and negative, drips to be degree, and 1132L/hm is amounted to the about 0.68L of soup in every sub-district
2During the spray medicine, manually block with plastic film every sub-district.
3.4 investigation and calculating
3.4.1 investigation method
Before for the first time spraying medicine, disease index, upgrowth situation and anomaly are respectively handled in investigation: for the second time, before the dispenser for the third time, observe incidence: investigate after dispenser for the third time 10 days at last, sick leaf rate, disease index and the control efficiency of investigation hemorrhagic black smallpox (branch, leaf, fruit ear), anthracnose (fruit ear).Do not fall ill during this experiment dispenser for the first time, disease index is 0.Blade (anthrachose of grape) stage division:
0 grade: no scab;
1 grade: 1～2 of scab is arranged on each blade;
3 grades: 3～4 of scabs are arranged on each blade;
5 grades: 5～6 of scabs are arranged on each blade;
7 grades: 6～10 of scabs are arranged on each blade;
9 grades: scab is arranged more than 10 on each blade, or scab links to each other and to account for blade area more than 1/4.
The fruit ear stage division:
0 grade: no scab;
1 grade: 1～2 of scab is arranged on each fruit ear;
3 grades: 3～4 of scabs are arranged on each fruit ear;
5 grades: 5～6 of scabs are arranged on each fruit ear;
7 grades: 6～10 of scabs are arranged on each fruit ear;
9 grades: scab is arranged more than 10 on each fruit ear, scab links to each other and accounts for the fruit area more than 1/4.
3.4.2 drug effect computational methods
Investigation result is carried out statistical analysis with the DMRT method
4, to other influences of grape
Duration of test does not see that poisoning takes place
5, test result analysis
Spray for the third time that medicine (June 13) back 10 days (June 23) is investigated and statistical analysis (see Table 1, table 2)
Table 1 " natural plant protection " is to the control efficiency of grape leave anthrachose of grape
Handle disease leaf rate (%) disease index preventive effect (%)
It 50 times of 1.5 0.83a 92.61a
It 30 times of 2.3 0.97a 90.45a
1000 times of 2.6 1.50a 86.21a of first holder
It 100 times of 4.5 3.63ab 62.45ab
It 200 times of 5.6 5.23b 47.61b
Clear water 11.8 10.40c
Table 2 " natural plant protection " is to the control efficiency of anthracnose
Handle disease tassel yield (%) disease index preventive effect (%)
It 50 times of 63.9 5.65a 74.81a
It 30 times of 63.3 6.18a 73.69a
1000 times of 59.9 10.63a 56.18a of first holder
It 100 times of 75.9 10.67a 56.91a
It 200 times of 53.5 11.30a 55.08a
Clear water 62.5 25.03b
6.1 natural plant protection is to the control efficiency of blade anthrachose of grape
Sick leaf rate: the sick leaf rate of " natural plant protection " 50 times of liquid, 30 times of liquid is respectively 1.05%, 2.3%, be lower than the methyl Tobe then 1000 times of liquid 2.6%; 100 times of liquid, 200 times of liquid are respectively 4.5%, 5.6%, be higher than then 1000 times of liquid of methyl Tobe, but each chemicals treatment all are lower than 11.8% of clear water.Show " natural plant protection " each concentration and methyl Tobe then 1000 times of liquid handle the incidence that all can reduce the anthrachose of grape of blade.
Disease index: the disease index of " natural plant protection " 30 times of liquid, 50 times of liquid, 100 times of liquid is respectively 0.97,0.83,3.63, significantly is lower than 5.23 of " natural plant protection " 200 times of liquid; " natural plant protection " 30 times of liquid, 50 times of liquid, 100 times of liquid handle and the methyl Tobe then 1000 times of liquid 1.50 between difference not remarkable; " natural plant protection " each concentration, methyl Tobe then 1000 times of liquid processing all significantly are lower than 10.40 of clear water contrast, show that " natural plant protection " each concentration processing all can effectively reduce the disease index of blade.And " natural plant protection " 50 times of liquid, 30 times of liquid reduce the effect of blade disease indexs and have and be better than contrasting the then trend of 1000 times of liquid of medicament methyl Tobe.
Control efficiency: the preventive effect of " natural plant protection " 50 times of liquid, 30 times of liquid, 100 times of liquid is respectively 92.61%, 90.45%, 62.45%, is significantly higher than 47.60% of " natural plant protection " 200 times; " natural plant protection " 50 times of liquid, 30 times of liquid, 100 times of liquid handle to the grape leave anthrachose of grape all have with the methyl Tobe then 1000 times of liquid phases with control efficiency, wherein the control efficiency of " natural plant protection " 50 times, 30 times liquid processing has to be better than the then trend of 1000 times of liquid of methyl Tobe more than 90%.
6.2 " natural plant protection " is to the control efficiency of anthracnose
The disease tassel yield of fruit ear anthracnose is respectively handled between 53.3%～63.9% in the test region, illustrates that the field anthracnose incidence of disease is higher.
Disease index: the disease index of " natural plant protection " 50 times of liquid, 30 times of liquid, 100 times of liquid, 200 times of liquid processing is respectively 5.65,6.18,10.67,11.30, and difference is not remarkable between each concentration; " natural plant protection " each concentration and methyl Tobe then 1000 times of liquid 10.63 between difference not remarkable, but significantly be lower than 20.03 of clear water.Showing that " natural plant protection " 50 times, 30 times has is better than the then trend of 1000 times of liquid of methyl Tobe.
Control efficiency: the preventive effect that " natural plant protection " 50 times, 30 times, 100 times, 200 times liquid are handled is respectively 74.81%, 73.69%, 59.91%, 55.08%, and difference was not remarkable between each concentration was handled; " natural plant protection " each concentration have with the methyl Tobe then 1000 times of liquid handle suitable preventive effects, wherein " natural plant protection " 50 times of liquid, 30 times of liquid are handled to have and are better than the then trend of 1000 times of liquid of methyl Tobe.
7, brief summary
From this test result analysis as can be known, " natural plant protection " 50 times of liquid, 30 times of liquid are handled the disease index that can significantly reduce anthrachose of grape, its preventive effect reaches 92.61%, 90.45% respectively, be better than conventional sterilization agent methyl Tobe then 1000 times of liquid 86.21%, be expected on producing, to be applied to prevent and treat anthrachose of grape.
Two, control grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease
Grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease etc. are the main fungal diseases of harm blade and fruit, take place in the strawberry producing region generally to cause dead seedling, decayed fruit, and be very big to yield effect.Entrusted by Chengdu Enwei Group, the pure Chinese herbal and crude drugs preparations of natural plant protection that the said firm is developed has carried out the control efficiency test to the strawberry disease, now test situation is summarized as follows.
1, materials and methods
1.1 field trial part
1.1.1 reagent agent:
Natural plant protection preparation (Chengdu Enwei Group provides)
Contrast medicament: 50% Sukeling WP (SUMITOMO CHEMICAL product, commercially available)
1.1.2 controlling object: grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease etc.
1.1.3 test is handled:
200 times in natural plant protection preparation
100 times in natural plant protection preparation
50 times in natural plant protection preparation
30 times in natural plant protection preparation
50% Sukeling WP1000 doubly
Blank
1.1.4 field design
Repeat 4 times 20 square metres of every sub-districts, and district's group is arranged at random.
1.1.5 dispenser and investigation
Dispenser for the first time on April 2 calendar year 2001, dispenser for the second time on April 9, BP-16 type manual sprayer spray medicine, 50 kilograms/mu of amount of liquid medicine.Investigation on April 16,5 samplings of diagonal (fruit), every 5 strain, every strain strawberry fruit is won entirely and carries out classification.Survey the weight of producing to the strong fruit of the every sub-district of weighing 15 strain strawberries.
Preventive effect is checked its otherness with the DMRT method after the arcsine conversion.
Attached: grade standard
0 grade: no scab;
1 grade: lesion area accounts for more than 5% of whole fruit area;
3 grades: lesion area accounts for 6%～15% of whole fruit area;
5 grades: lesion area accounts for 16%～25% of whole fruit area;
7 grades: lesion area accounts for 26%～50% of whole fruit area;
9 grades: lesion area accounts for more than 50% of whole fruit area.
1.1.6 experimental field basic condition and climatic condition:
Experimental field soil belongs to yellow earth, and middle fertility, strawberry cultivars are fragrant strawberry.Field strawberrying gray mold fruit, indoorly carry out separation and purification, expand numerous, again with mycelia and medium with bruisher smash to pieces, spray inoculation, pour water between ditch and preserve moisture, lure morbidity into.
1.2 indoor antibacterial test
1.2.1 reagent agent: natural plant protection preparation (Chengdu Enwei Group provides)
Contrast medicament: 50% Sukeling WP (SUMITOMO CHEMICAL product)
The upright agent (precious jade peak, county product falls in Shanxi) of cutting off the water of 12% bacterium
1.2.2 test is handled:
(1) natural plant protection preparation is 10 times
(2) natural plant protection preparation is 20 times
(3) natural plant protection preparation is 50 times
(4) natural plant protection preparation is 100 times
(5) natural plant protection preparation is 200 times
(6) natural plant protection preparation is 500 times
(7) natural plant protection preparation is 1000 times
(8) natural plant protection preparation is 1500 times
(9) 50% Sukeling WP1000 doubly
(10) 50% Sukeling WP2000 doubly
Upright 1000 times of the agent of cutting off the water of (11) 12% bacterium
Upright 2000 times of the agent of cutting off the water of (12) 12% bacterium
(13) blank
1.2.3 test method adopts the growth rate determination method, concrete operations are as follows:
(1) the sick fruit of pathogen in field strawberrying gray mold cultivated in separation and purification, then separation and purification and cultivate standby.
(2) preparation pastille medium plane is accurately drawn a certain amount of soup and is joined in the medium of thawing, mix in the culture dish of pouring sterilization into, the cooling back pastille medium plane.
(3) the inoculation pathogen is adopted bacterium cake inoculation method, beats with the card punch of 6mm and getting the cultivation pilum (bacterium cake) that carries disease germs in (1), is put on the pastille medium plane with transfer needle gripping bacterium cake then, puts a bacterium cake in each culture dish.
(4) after computational methods are cultivated 48h and 72h in 25 ℃ of incubators, take out culture dish and measure colony diameter.Each bacterium colony is measured diameter with the right-angled intersection method, institute's survey diameter is deducted bacterium cake diameter promptly get colony diameter, represents the bacterium colony size with the mean of colony diameter.
2. test results and analysis
2.1 field trial part (result of the test sees Table 1)
200 times, 100 times, 50 times, 30 times average preventive effects to grey mould fruit rot of strawberry of natural plant protection preparation are 50.22%, 66.85%, 72.22%, 80.85%, and contrast medicament 50% Sukeling WP1000 is 81.96% to the average preventive effect of grey mould fruit rot of strawberry doubly.Preventive effect through arcsine conversion back with the check of DMRT method, on 5% significance level, except that handle between 30 times and contrast medicament and 50 times and 100 times difference remarkable, it is remarkable that all the other respectively handle differences.
2.2 indoor antibacterial test part (result of the test see Table 2 and table 3)
After cultivating 48h, 10 times, 20 times, 50 times, 100 times, 200 times inhibition growth rates to grey mould fruit rot of strawberry of natural plant protection preparation are respectively 100%, 100%, 80.65%, 62.83% and 43.78%, other of natural plant protection preparation handled equal unrestraint effect, contrast medicament 50% Sukeling WP1000 is that upright 1000 times and the 2000 times inhibition growth rates to the grey mould fruit rot of strawberry bacterium of agent of cutting off the water of 74.34% and 68.57%, 12% bacterium are respectively 85.84% and 79.19% with 2000 times of inhibition growth rates to the grey mould fruit rot of strawberry bacterium doubly; Cultivate after 72 hours, 10 times, 20 times, 50 times, 100 times, 200 times inhibition growth rates to grey mould fruit rot of strawberry of natural plant protection preparation are respectively 100%, 100%, 85.83%, 71%, 55.96%, 500 times, 1000 times in natural plant protection preparation and 1500 times of then unrestraint effects, contrast medicament 50% Sukeling WP1000 is that upright 1000 times and the 2000 times inhibition growth rates to the grey mould fruit rot of strawberry bacterium of agent of cutting off the water of 83.37% and 81.13%, 12% bacterium are respectively 91.68% and 88.08% with 2000 times of inhibition growth rates to the grey mould fruit rot of strawberry bacterium doubly.
3. conclusions and recommendations
Field test results can find out, multiple diseases such as natural plant protection preparation control grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease have certain effect, except that 30 times with the contrast pharmacy control efficacy quite, other all is lower than the contrast medicament; Indoor antibacterial test is the result show, the inhibition growth rate that the concentration after 100 times is handled the grey mould fruit rot of strawberry bacterium all is starkly lower than contrast medicament 50% Sukeling WP and the upright agent of cutting off the water of 12% bacterium.Natural plant protection preparation has the advantage of nontoxic residue-free, can be used for nuisanceless fruit production.
Table 1 field trial
| Handle | Repeat | Disease refers to | Preventive effect % |
| 200 times in natural plant protection preparation | ????1 | ????13.76 | ????42.95 |
| ????2 | ????11.67 | ????51.62 | |
| ????3 | ????11.28 | ????53.23 | |
| ????4 | ????11.33 | ????53.07 | |
| Mean value | ????12.01 | ????50.22 | |
| 100 times in natural plant protection preparation | ????1 | ????6.89 | ????71.42 |
| ????2 | ????8.65 | ????64.14 | |
| ????3 | ????9.02 | ????62.06 | |
| ????4 | ????7.42 | ????69.24 | |
| Mean value | ????8.00 | ????66.85 | |
| 50 times in natural plant protection preparation | ????1 | ????5.92 | ????75.45 |
| ????2 | ????6.96 | ????71.14 | |
| ????3 | ????7.34 | ????69.57 | |
| ????4 | ????6.62 | ????72.55 | |
| Mean value | ????6.71 | ????72.22 | |
| 30 times in natural plant protection preparation | ????1 | ????4.12 | ????82.92 |
| ????2 | ????5.03 | ????79.15 | |
| ????3 | ????4.47 | ????81.48 | |
| ????4 | ????4.86 | ????79.85 |
| Mean value | ????4.62 | ????80.85 | |
| 50% Sukeling WP1000 doubly | ????1 | ????3.40 | ????84.12 |
| ????2 | ????5.93 | ????75.41 | |
| ????3 | ????3.76 | ????84.41 | |
| ????4 | ????3.88 | ????83.91 | |
| Mean value | ????4.24 | ????81.96 | |
| ?CK | ????1 | ????21.42 | ????- |
| ????2 | ????21.54 | ????- | |
| ????3 | ????28.02 | ????- | |
| ????4 | ????25.51 | ????- | |
| Mean value | ????24.12 |
Table 2: cultivate the colony diameter (mm) after 48 hours
Table 3: cultivate the colony diameter (mm) after 72 hours
Three, carried out the test of control vegetables, fruit tree fungal disease, virus disease and aphid, respond well.
Claims (7)
1. plant insecticide disease-prevention growth promoter and preparation method thereof is characterized in that the plant insecticide disease-prevention growth promoter is liquor or paste or the pulvis that contains Berberine hydrochloride 0.06～0.36%, matrine 0.01～0.06%, general flavone 0.23～1.38%.
2. plant insecticide disease-prevention growth promoter according to claim 1 and preparation method thereof, the preparation method who it is characterized in that the plant insecticide disease-prevention growth promoter, be by weight primary crude drug to be mixed, main primary crude drug is 1 part of golden cypress, 0.25～3.5 part on tobacco leaf, 0.25～4 part of kuh-seng, 0.15～2 part of azedarach, the water that per 1 part of primary crude drug adding is 8～12 parts decocts 2～3 times, each 45～70 minutes, collect volatile oil simultaneously and get soup, with medical filtration, be concentrated into relative density 1.05～1.3, obtain plant insecticide disease-prevention growth promoter liquor.
3. plant insecticide disease-prevention growth promoter according to claim 2 and preparation method thereof is characterized in that also can adding in the primary crude drug 0.15～2 part of sweet wormwood.
4. plant insecticide disease-prevention growth promoter according to claim 2 and preparation method thereof is characterized in that also can adding in the primary crude drug 0.15～1.5 part of mother chrysanthemum.
5. plant insecticide disease-prevention growth promoter according to claim 3 and preparation method thereof is characterized in that also can adding in the primary crude drug 0.15～1.5 part of mother chrysanthemum.
6. according to claim 2,3,4,5 described plant insecticide disease-prevention growth promoters and preparation method thereof, it is characterized in that the plant insecticide disease-prevention growth promoter liquor that obtains adds 0.2～0.4% aromatic, 2～4% preservatives, 3～6% synergist in proportion and promptly gets plant insecticide disease-prevention growth promoter paste.
7. according to claim 2,3,4,5 described plant insecticide disease-prevention growth promoters and preparation method thereof, it is characterized in that the plant insecticide disease-prevention growth promoter liquor that obtains is spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410022134XA CN1281136C (en) | 2004-03-26 | 2004-03-26 | Pesticidal disease-preventing plant growth promoter and its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410022134XA CN1281136C (en) | 2004-03-26 | 2004-03-26 | Pesticidal disease-preventing plant growth promoter and its prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1672520A true CN1672520A (en) | 2005-09-28 |
| CN1281136C CN1281136C (en) | 2006-10-25 |
Family
ID=35045493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200410022134XA Expired - Fee Related CN1281136C (en) | 2004-03-26 | 2004-03-26 | Pesticidal disease-preventing plant growth promoter and its prepn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1281136C (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100581367C (en) * | 2007-05-11 | 2010-01-20 | 山东省农业科学院植物保护研究所 | Compound against plant virus |
| CN102696699A (en) * | 2012-06-07 | 2012-10-03 | 海南大学 | Murraya paniculata (L.) total flavones soluble powder and preparation method thereof |
| CN102986749A (en) * | 2012-12-26 | 2013-03-27 | 李香侠 | Plant protecting agent and preparation method thereof |
| WO2013043031A1 (en) * | 2011-09-23 | 2013-03-28 | Promotora Tecnica Industrial S.A. De C.V. | Pesticide made of isoquinoline alkaloids, flavonoids and vegetable and/or essential oils |
| CN103814966A (en) * | 2014-03-05 | 2014-05-28 | 王宜磊 | Peony cultivation special patch for preventing fungus diseases and preparation method thereof |
| CN104938540A (en) * | 2015-07-17 | 2015-09-30 | 曾建民 | Preparation method for mosquito and other insect repellent |
| CN104957192A (en) * | 2015-07-17 | 2015-10-07 | 曾建民 | Liquid repellent for mosquitoes |
| CN105707128A (en) * | 2016-03-09 | 2016-06-29 | 高小文 | Pesticide composition containing tea saponin and preparation method and application of pesticide composition |
| CN105994422A (en) * | 2016-06-17 | 2016-10-12 | 枞阳县白云生态园林有限责任公司 | Olive leaf and grape botanical pesticide |
| CN107302917A (en) * | 2016-04-21 | 2017-10-31 | 上海新舒环环保科技有限公司 | It is a kind of to prevent and treat the preparation method that pythium spp realizes soil remediation |
| CN108575671A (en) * | 2018-04-11 | 2018-09-28 | 合肥卓畅农业科技有限公司 | A kind of insect prevention organic nutrient soil of safety and environmental protection |
| CN110402741A (en) * | 2019-07-31 | 2019-11-05 | 广西澳一澳农业科技有限公司 | A kind of high yield macadamia implantation methods |
| CN113951285A (en) * | 2021-10-13 | 2022-01-21 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
-
2004
- 2004-03-26 CN CNB200410022134XA patent/CN1281136C/en not_active Expired - Fee Related
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100581367C (en) * | 2007-05-11 | 2010-01-20 | 山东省农业科学院植物保护研究所 | Compound against plant virus |
| WO2013043031A1 (en) * | 2011-09-23 | 2013-03-28 | Promotora Tecnica Industrial S.A. De C.V. | Pesticide made of isoquinoline alkaloids, flavonoids and vegetable and/or essential oils |
| CN103687491A (en) * | 2011-09-23 | 2014-03-26 | 助剂技术工业有限公司 | Pesticide made of isoquinoline alkaloids, flavonoids and vegetable and/or essential oils |
| CN103687491B (en) * | 2011-09-23 | 2016-08-17 | 助剂技术工业有限公司 | Based on isoquinoline alkaloids bases and flavonoid have insecticide, acaricide and the pesticide of nematicide activity |
| CN102696699A (en) * | 2012-06-07 | 2012-10-03 | 海南大学 | Murraya paniculata (L.) total flavones soluble powder and preparation method thereof |
| CN102986749A (en) * | 2012-12-26 | 2013-03-27 | 李香侠 | Plant protecting agent and preparation method thereof |
| CN103814966A (en) * | 2014-03-05 | 2014-05-28 | 王宜磊 | Peony cultivation special patch for preventing fungus diseases and preparation method thereof |
| CN103814966B (en) * | 2014-03-05 | 2015-09-02 | 菏泽学院 | A kind of Peony growing special-purpose plaster preventing and treating fungal disease and preparation method thereof |
| CN104957192A (en) * | 2015-07-17 | 2015-10-07 | 曾建民 | Liquid repellent for mosquitoes |
| CN104938540A (en) * | 2015-07-17 | 2015-09-30 | 曾建民 | Preparation method for mosquito and other insect repellent |
| CN104957192B (en) * | 2015-07-17 | 2018-02-27 | 泉州立唯体育场地设施有限公司 | A kind of shoe cover of field work for mosquito repellent |
| CN105707128A (en) * | 2016-03-09 | 2016-06-29 | 高小文 | Pesticide composition containing tea saponin and preparation method and application of pesticide composition |
| CN105707128B (en) * | 2016-03-09 | 2018-10-02 | 高小文 | A kind of composition pesticide and preparation method and application containing tea saponin |
| CN107302917A (en) * | 2016-04-21 | 2017-10-31 | 上海新舒环环保科技有限公司 | It is a kind of to prevent and treat the preparation method that pythium spp realizes soil remediation |
| CN105994422A (en) * | 2016-06-17 | 2016-10-12 | 枞阳县白云生态园林有限责任公司 | Olive leaf and grape botanical pesticide |
| CN108575671A (en) * | 2018-04-11 | 2018-09-28 | 合肥卓畅农业科技有限公司 | A kind of insect prevention organic nutrient soil of safety and environmental protection |
| CN110402741A (en) * | 2019-07-31 | 2019-11-05 | 广西澳一澳农业科技有限公司 | A kind of high yield macadamia implantation methods |
| CN113951285A (en) * | 2021-10-13 | 2022-01-21 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1281136C (en) | 2006-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1319460C (en) | Chinese herbal medicine germicide | |
| CN1047200C (en) | Earth-cultivated black fungus and its cultivating method by use of substitute material for plastic bag | |
| CN1281136C (en) | Pesticidal disease-preventing plant growth promoter and its prepn | |
| CN100351371C (en) | Antiseptic antistaling agent containing hay bacillocin | |
| CN1847200A (en) | Pollution-less pesticide fertilizer for preventing and controlling blight and its production process | |
| CN1806495A (en) | Method for cultivating edible fungus by utilizing exotic invasive weed | |
| CN1740175A (en) | Plumepoppy total alkaloid salt and its prepn and application | |
| CN1736186A (en) | Method for breeding fly maggot with anti-tumor biological characteristic | |
| KR100823541B1 (en) | Mushroom cultivation method | |
| Zhou | Cultivation of Ganoderma lucidum | |
| CN1810259A (en) | Gallnut medicine for preventing and treating caries and its extraction process | |
| CN1699297A (en) | Process for preparing organic liquid fertilizer and organic solid fertilizer | |
| CN105198650A (en) | Nutrient soil for clivia minata and preparation method of nutrient soil | |
| CN112931052A (en) | Morchella cultivation medium and preparation method thereof | |
| CN101050209A (en) | Compound of eudesmane type sesquiterpene lactone with insecticidal activity extracted from Carpesium macrocephalum, and application | |
| CN1301060C (en) | Plant Source non-public hazard biological insect repellant and preparing process | |
| CN1112843C (en) | Cinnamomum subavenium tissue culture and quick reproduction method | |
| CN1853483A (en) | Green trichodermin enoyl morpholine water dispersion tablet or granule and production thereof | |
| CN1623373A (en) | Cremastra appendiculata in vitro culturing and fast reproducing biotechnological method | |
| CN1207032C (en) | Chinese medicine composition for curing acute and chronic rhinorrhea with turbid discharge and its preparation method | |
| CN100342787C (en) | Combination of insect disinfectations containing benzoate of Avermectin in methylamine group and azadirachtin | |
| CN1433695A (en) | Plant source bacteriocide for preventing and controlling plant disease and preparation method thereof | |
| KR20200012367A (en) | How to grow flower mushrooms | |
| TWI784235B (en) | A method for promoting the generation of new shoots at plant growth points, inhibiting the growth of terminal buds and increasing the number of side branches, and the products made by the method | |
| KR101637164B1 (en) | The inducing method of primordium of Grifola frondosa(mushroom) using pot |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061025 |