CN1281136C - Pesticidal disease-preventing plant growth promoter and its prepn - Google Patents
Pesticidal disease-preventing plant growth promoter and its prepn Download PDFInfo
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- CN1281136C CN1281136C CNB200410022134XA CN200410022134A CN1281136C CN 1281136 C CN1281136 C CN 1281136C CN B200410022134X A CNB200410022134X A CN B200410022134XA CN 200410022134 A CN200410022134 A CN 200410022134A CN 1281136 C CN1281136 C CN 1281136C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to a pesticidal disease-preventing plant growth promoter and a preparation method thereof, wherein the pesticidal disease-preventing plant growth promoter is liquor or paste or powder containing 0.06 to 0.36 % of berberine hydrochloride, 0.01 to 0.06 % of matrine and 0.23 to 1.38 % of total flavonoid; the pesticidal disease-preventing plant growth promoter has the preparation method that: mixing the following main raw medicinal material part by weight: 1 part of amur corktree bark, 0.25 to 3.5 parts of tobacco leaf, 0.25 to 4 parts of flavescent sophora root and 0.15 to 2 parts of chinaberry bark, decocting the raw medicinal material for 2 to 3 times for 45 to 70 minutes each time by adding 8 to 12 parts of water to each part of raw medicinal material, simultaneously collecting volatile oil to obtain physic liquor, filtering the physic liquor, and concentrating the physic liquor to the relative density of 1.05 to 1.3 to obtain the pesticidal disease-preventing plant growth promoter. The pesticidal disease-preventing plant growth promoter of the present invention has the advantages of preferable control efficiency of various plant diseases and insect pests, no toxicity, no residue, simple preparation method, simple technology and low cost and can be used for producing pollution-free vegetables and fruits.
Description
Affiliated technical field: the invention belongs to pesticide field, specifically is a kind of plant insecticide disease-prevention growth promoter and preparation method thereof.
Background technology: along with the development of society, the arrival of industrial civilization has brought impayable material wealth to the mankind.Yet, the unification of man and nature, coordination, the ecological balance have been suffered great destruction under the situation that with people's will is not transfer, human owing to too much eaten food such as the vegetables of having used chemical pesticide, fruit, persticide residue because of chemical pesticide, the mankind have been brought a series of disease, as developed as one pleases and to cause serious consequence to the mankind.
Summary of the invention:
The purpose of this invention is to provide a kind of plant insecticide disease-prevention growth promoter and preparation method thereof, the plant insecticide disease-prevention growth promoter has the better prevention effect to multiple damage by disease and insect, has the advantage of nontoxic residue-free, can be used for pollution-free vegetable, fruit production, its preparation method technology is simple, and is with low cost.
The objective of the invention is to realize by following technical proposals:
Plant insecticide disease-prevention growth promoter of the present invention is liquor or paste or the pulvis that contains Berberine hydrochloride 0.06～0.36%, matrine 0.01～0.06%, general flavone 0.23～1.38%.
The preparation method of plant insecticide disease-prevention growth promoter of the present invention, be by weight primary crude drug to be mixed, main primary crude drug is 1 part of golden cypress, 0.25～3.5 part on tobacco leaf, 0.25～4 part of kuh-seng, 0.15～2 part of azedarach, the water that per 1 part of primary crude drug adding is 8～12 parts decocts 2～3 times, each 45～70 minutes, collect simultaneously volatile oil and soup, with medical filtration, be concentrated into relative density 1.05～1.3, obtain plant insecticide disease-prevention growth promoter liquor.
In the such scheme, also can add 0.15～2 part of sweet wormwood in the primary crude drug.
In the such scheme, also can add 0.15～1.5 part of mother chrysanthemum in the primary crude drug.
In the such scheme, the plant insecticide disease-prevention growth promoter liquor that obtains adds 0.2～0.4% aromatic, 2～4% preservatives, 3～6% synergist by weight proportion and promptly gets plant insecticide disease-prevention growth promoter paste.
In the such scheme, the plant insecticide disease-prevention growth promoter liquor that obtains is spray dried to extract powder with spray drying tower, adds 8% sodium bicarbonate, 10% fumaric acid fine powder by weight proportion, mixes promptly to get plant insecticide disease-prevention growth promoter pulvis.
The present invention utilizes Berberine hydrochloride and the matrine that extracts in the primary crude drug, and general flavone administers the damage by disease and insect of plant, and is promoted the growth of plant by the general flavone that extracts.
The present invention changes chemical pesticide by using plant pesticide, and reduces human because of the damage of agricultural chemicals residual in the vegetables of having taken the use chemical pesticide, the fruit to health.Plant insecticide disease-prevention growth promoter of the present invention be primarily aimed at vegetables, fruit tree fungi, virus, aphid control and promote plant growing.
Therefore, plant insecticide disease-prevention growth promoter of the present invention has the better prevention effect to multiple damage by disease and insect, has the advantage of nontoxic residue-free, can be used for pollution-free vegetable, fruit production; Its preparation method technology is simple, and is with low cost.
Embodiment:
The invention is further illustrated by the following examples, and the present invention is not limited only to described embodiment.
Embodiment one
Get golden cypress 30kg, tobacco leaf 25kg, kuh-seng 30kg, azedarach 15kg decocts secondary with above-mentioned primary crude drug mixture, adds the drinking water of 1200L at every turn, each 45 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.05～1.08, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.15%, matrine 0.02%, general flavone 0.43%.Add 0.2% aromatic, 2% preservative, the clear paste of the formulated plant insecticide disease-prevention growth promoter of 3% synergist again.
Embodiment two
Get golden cypress 60kg, tobacco leaf 15kg, kuh-seng 15kg, azedarach 10kg, above-mentioned primary crude drug mixture is decocted secondary, boil after for the first time soaking 25min, add the drinking water of 800L at every turn, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.20～1.30, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.06%, matrine 0.01%, general flavone 0.23%.Add 0.4% aromatic, 4% preservative, the agent of the formulated plant insecticide disease-prevention growth promoter of 6% synergist thick paste again.
Embodiment three
Get golden cypress 7.2kg, tobacco leaf 25kg, kuh-seng 28.5kg, azedarach 14.4kg, sweet wormwood 14.4kg, mother chrysanthemum 10.5kg decocts above-mentioned primary crude drug mixture three times, boils after for the first time soaking 40min, each drinking water that adds 800L, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature are concentrated into relative density 1.10, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.36%, matrine 0.06%, general flavone 1.38%, be spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
Embodiment four
Get golden cypress 30kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 25.5kg, sweet wormwood 4.5kg decocts secondary with above-mentioned primary crude drug mixture, boils after for the first time soaking 30min, adds the drinking water of 1000L at every turn, each 60 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.05～1.08, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.16%, matrine 0.02%, general flavone 0.82%.Add 0.3% aromatic, 3% preservative, the clear paste of the formulated plant insecticide disease-prevention growth promoter of 4% synergist again.
Embodiment five
Get golden cypress 30kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 25.5kg, mother chrysanthemum 4.5kg decocts secondary with above-mentioned primary crude drug mixture, boils after for the first time soaking 30min, adds the drinking water of 800L at every turn, each 70 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, and two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature, be concentrated into relative density 1.20～1.30, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.16%, matrine 0.02%, general flavone 0.83%.Add 0.3% aromatic, 3% preservative, the agent of the formulated plant insecticide disease-prevention growth promoter of 5% synergist thick paste again.
Embodiment six
Get golden cypress 15kg, tobacco leaf 20kg, kuh-seng 20kg, azedarach 15kg, sweet wormwood 15kg, mother chrysanthemum 15kg decocts above-mentioned primary crude drug mixture three times, boils after for the first time soaking 30min, each drinking water that adds 800L, each 60 minutes, collect volatile oil simultaneously, again with 200 mesh sieves filter soup; With the energy-conservation inspissator concentrated medicament of triple effect, one imitates 80 ℃ of temperature, two imitate 70 ℃ of temperature, 60 ℃ of triple effect temperature are concentrated into relative density 1.10, obtain plant insecticide disease-prevention growth promoter liquor, composition is Berberine hydrochloride 0.26%, matrine 0.05%, general flavone 1.03%, be spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
The plant insecticide disease-prevention growth promoter (code name is a vegetable insecticide) of the present invention's preparation is as follows in the field control effectiveness test result who prevents and treats disease:
One, prevents and treats anthracnose of grape, anthracnose
1, experiment purpose
" vegetable insecticide " is a kind of novel plant source biological pesticide, and anthracnose of grape, anthracnose are two big main diseases of northern China viny region, and extensively cause harm grape young sprout, blade, fruit are the key factors that causes the grape underproduction and reduce quality.This experiment is used " vegetable insecticide " in the grape growth phase, contrasts as medicament with conventional sterilization agent thiophanate methyl simultaneously, with the control efficiency of checking " vegetable insecticide " to anthracnose of grape, anthracnose.
2, experimental condition
2.1 trial crops
Seeds: grape variety: auspicious Bill (be commonly called as: American Black is carried)
The age of tree: life in 2 years
2.2 controlling object
Anthrachose of grape, anthracnose
3, test method
3.1 medicament
3.1.1 test medicine
" vegetable insecticide " botanical biological pesticide stoste is provided by Chengdu En Wei group, establishes totally 4 the concentration processing of 30 times, 50 times, 100 times, 200 times liquid.
3.1.2 contrast medicament
The compound wetting powder of thiophanate methyl 60% ultra micro
3.1.3 blank
Clear water is a blank
3.2 sub-district design
5 strains of every sub-district, area 10m
2, district's group is arranged at random, repeats 4 times.
3.3 dispenser period and method
For the first time spraying time May 23,, in June 3, each spray on June 13 once, spray medicine altogether 3 times, spray medicine at every turn and all before at 12 o'clock in the morning, finished later on according to weather conditions.The spraying machine tool adopts conventional knapsack hand sprayer, and every strain even spraying is moistening to the blade face positive and negative, drips to be degree, and 1132L/hm is amounted to the about 0.68L of soup in every sub-district
2During the spray medicine, manually block with plastic film every sub-district.
3.4 investigation and calculating
3.4.1 investigation method
Before for the first time spraying medicine, disease index, upgrowth situation and anomaly are respectively handled in investigation: for the second time, before the dispenser for the third time, observe incidence: investigate after dispenser for the third time 10 days at last, sick leaf rate, disease index and the control efficiency of investigation hemorrhagic black smallpox (branch, leaf, fruit ear), anthracnose (fruit ear).Do not fall ill during this experiment dispenser for the first time, disease index is 0.Blade (anthrachose of grape) stage division:
0 grade: no scab;
1 grade: 1～2 of scab is arranged on each blade;
3 grades: 3～4 of scabs are arranged on each blade;
5 grades: 5～6 of scabs are arranged on each blade;
7 grades: 6～10 of scabs are arranged on each blade;
9 grades: scab is arranged more than 10 on each blade, or scab links to each other and to account for blade area more than 1/4.
The fruit ear stage division:
0 grade: no scab;
1 grade: 1～2 of scab is arranged on each fruit ear;
3 grades: 3～4 of scabs are arranged on each fruit ear;
5 grades: 5～6 of scabs are arranged on each fruit ear;
7 grades: 6～10 of scabs are arranged on each fruit ear; 9 grades: scab is arranged more than 10 on each fruit ear, scab links to each other and accounts for the fruit area more than 1/4.
3.4.2 drug effect computational methods
Investigation result is carried out statistical analysis with the DMRT method
4, to other influences of grape
Duration of test does not see that poisoning takes place
5, test result analysis
Spray for the third time that medicine (June 13) back 10 days (June 23) is investigated and statistical analysis (see Table 1, table 2)
Table 1 " vegetable insecticide " is to the control efficiency of grape leave anthrachose of grape
| Handle | Sick leaf rate (%) | Disease index | Preventive effect (%) |
| Planting 50 times plants 1000 times of 30 times of first holders and plants 100 times and plant 200 times of clear water | 1.5 2.3 2.6 4.5 5.6 11.8 | 0.83a 0.97a 1.50a 3.63ab 5.23b 10.40c | 92.61a 90.45a 86.21a 62.45ab 47.61b |
Table 2 " vegetable insecticide " is to the control efficiency of anthracnose
| Handle | Disease tassel yield (%) | Disease index | Preventive effect (%) |
| Planting 50 times plants 1000 times of 30 times of first holders and plants 100 times and plant 200 times of clear water | 63.9 63.3 59.9 75.9 53.5 62.5 | 5.65a 6.18a 10.63a 10.67a 11.30a 25.03b | 74.81a 73.69a 56.18a 56.91a 55.08a |
6.1 vegetable insecticide is to the control efficiency of blade anthrachose of grape
Sick leaf rate: the sick leaf rate of " vegetable insecticide " 50 times of liquid, 30 times of liquid is respectively 1.05%, 2.3%, is lower than 2.6% of 1000 times of liquid of thiophanate methyl; 100 times of liquid, 200 times of liquid are respectively 4.5%, 5.6%, be higher than 1000 times of liquid of thiophanate methyl, but each chemicals treatment all are lower than 11.8% of clear water.Show that 1000 times of liquid processing of " vegetable insecticide " each concentration and thiophanate methyl all can reduce the incidence of the anthrachose of grape of blade.
Disease index: the disease index of " vegetable insecticide " 30 times of liquid, 50 times of liquid, 100 times of liquid is respectively 0.97,0.83,3.63, significantly is lower than 5.23 of " vegetable insecticide " 200 times of liquid; " vegetable insecticide " 30 times of liquid, 50 times of liquid, 100 times of liquid handle and 1000 times of liquid of thiophanate methyl 1.50 between difference not remarkable; " vegetable insecticide " each concentration, 1000 times of liquid of thiophanate methyl are handled and all significantly are lower than 10.40 of clear water contrast, show that " vegetable insecticide " each concentration processing all can effectively reduce the disease index of blade.And the effect of " vegetable insecticide " 50 times of liquid, 30 times of liquid reduction blade disease indexs has the trend that is better than contrasting 1000 times of liquid of medicament thiophanate methyl.
Control efficiency: the preventive effect of " vegetable insecticide " 50 times of liquid, 30 times of liquid, 100 times of liquid is respectively 92.61%, 90.45%, 62.45%, is significantly higher than 47.60% of " vegetable insecticide " 200 times; " vegetable insecticide " 50 times of liquid, 30 times of liquid, 100 times of liquid processing all have the grape leave anthrachose of grape and 1000 times of liquid phases of thiophanate methyl control efficiency together, wherein the control efficiency of " vegetable insecticide " 50 times, 30 times liquid processing has the trend that is better than 1000 times of liquid of thiophanate methyl more than 90%.
6.2 " vegetable insecticide " is to the control efficiency of anthracnose
The disease tassel yield of fruit ear anthracnose is respectively handled between 53.3%～63.9% in the test region, illustrates that the field anthracnose incidence of disease is higher.
Disease index: the disease index of " vegetable insecticide " 50 times of liquid, 30 times of liquid, 100 times of liquid, 200 times of liquid processing is respectively 5.65,6.18,10.67,11.30, and difference is not remarkable between each concentration; " vegetable insecticide " each concentration and 1000 times of liquid of thiophanate methyl 10.63 between difference not remarkable, but significantly be lower than 20.03 of clear water.Show that " vegetable insecticide " 50 times, 30 times have the trend that is better than 1000 times of liquid of thiophanate methyl.
Control efficiency: the preventive effect that " vegetable insecticide " 50 times, 30 times, 100 times, 200 times liquid are handled is respectively 74.81%, 73.69%, 59.91%, 55.08%, and difference was not remarkable between each concentration was handled; " vegetable insecticide " each concentration has with 1000 times of liquid of thiophanate methyl handles suitable preventive effect, and wherein " vegetable insecticide " 50 times of liquid, 30 times of liquid processing have the trend that is better than 1000 times of liquid of thiophanate methyl.
7, brief summary
From this test result analysis as can be known, " vegetable insecticide " 50 times of liquid, 30 times of liquid are handled the disease index that can significantly reduce anthrachose of grape, its preventive effect reaches 92.61%, 90.45% respectively, be better than 86.21% of 1000 times of liquid of conventional sterilization agent thiophanate methyl, be expected on producing, to be applied to prevent and treat anthrachose of grape.
Two, control grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease
Grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease etc. are the main fungal diseases of harm blade and fruit, take place in the strawberry producing region generally to cause dead seedling, decayed fruit, and be very big to yield effect.Entrusted by Chengdu Enwei Group, the pure Chinese herbal and crude drugs preparations of vegetable insecticide that the said firm is developed has carried out the control efficiency test to the strawberry disease, now test situation is summarized as follows.
1, materials and methods
1.1 field trial part
1.1.1 reagent agent:
Plant insecticide agent formulation (Chengdu Enwei Group provides)
Contrast medicament: 50% Sukeling WP (SUMITOMO CHEMICAL product, commercially available)
1.1.2 controlling object: grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease etc.
1.1.3 test is handled:
200 times of plant insecticide agent formulations
100 times of plant insecticide agent formulations
50 times of plant insecticide agent formulations
30 times of plant insecticide agent formulations
50% Sukeling WP1000 doubly
Blank
1.1.4 field design
Repeat 4 times 20 square metres of every sub-districts, and district's group is arranged at random.
1.1.5 dispenser and investigation
Dispenser for the first time on April 2 calendar year 2001, dispenser for the second time on April 9, BP-16 type manual sprayer spray medicine, 50 kilograms/mu of amount of liquid medicine.Investigation on April 16,5 samplings of diagonal (fruit), every 5 strain, every strain strawberry fruit is won entirely and carries out classification.Survey the weight of producing to the strong fruit of the every sub-district of weighing 15 strain strawberries.
Preventive effect is checked its otherness with the DMRT method after the arcsine conversion.
Attached: grade standard
0 grade: no scab;
1 grade: lesion area accounts for more than 5% of whole fruit area;
3 grades: lesion area accounts for 6%～15% of whole fruit area;
5 grades: lesion area accounts for 16%～25% of whole fruit area;
7 grades: lesion area accounts for 26%～50% of whole fruit area;
9 grades: lesion area accounts for more than 50% of whole fruit area.
1.1.6 experimental field basic condition and climatic condition:
Experimental field soil belongs to yellow earth, and middle fertility, strawberry cultivars are fragrant strawberry.Field strawberrying gray mold fruit, indoorly carry out separation and purification, expand numerous, again with mycelia and medium with bruisher smash to pieces, spray inoculation, pour water between ditch and preserve moisture, lure morbidity into.
1.2 indoor antibacterial test
1.2.1 reagent agent: plant insecticide agent formulation (Chengdu Enwei Group provides)
Contrast medicament: 50% Sukeling WP (SUMITOMO CHEMICAL product)
The upright agent (precious jade peak, county product falls in Shanxi) of cutting off the water of 12% bacterium
1.2.2 test is handled:
(1) the plant insecticide agent formulation is 10 times
(2) the plant insecticide agent formulation is 20 times
(3) the plant insecticide agent formulation is 50 times
(4) the plant insecticide agent formulation is 100 times
(5) the plant insecticide agent formulation is 200 times
(6) the plant insecticide agent formulation is 500 times
(7) the plant insecticide agent formulation is 1000 times
(8) the plant insecticide agent formulation is 1500 times
(9) 50% Sukeling WP1000 doubly
(10) 50% Sukeling WP2000 doubly
Upright 1000 times of the agent of cutting off the water of (11) 12% bacterium
Upright 2000 times of the agent of cutting off the water of (12) 12% bacterium
(13) blank
1.2.3 test method adopts the growth rate determination method, concrete operations are as follows:
(1) the sick fruit of pathogen in field strawberrying gray mold cultivated in separation and purification, then separation and purification and cultivate standby.
(2) preparation pastille medium plane is accurately drawn a certain amount of soup and is joined in the medium of thawing, mix in the culture dish of pouring sterilization into, the cooling back pastille medium plane.
(3) the inoculation pathogen is adopted bacterium cake inoculation method, beats with the card punch of 6mm and getting the cultivation pilum (bacterium cake) that carries disease germs in (1), is put on the pastille medium plane with transfer needle gripping bacterium cake then, puts a bacterium cake in each culture dish.
(4) after computational methods are cultivated 48h and 72h in 25 ℃ of incubators, take out culture dish and measure colony diameter.Each bacterium colony is measured diameter with the right-angled intersection method, institute's survey diameter is deducted bacterium cake diameter promptly get colony diameter, represents the bacterium colony size with the mean of colony diameter.
2. test results and analysis
2.1 field trial part (result of the test sees Table 1)
200 times, 100 times, 50 times, 30 times average preventive effects to grey mould fruit rot of strawberry of plant insecticide agent formulation are 50.22%, 66.85%, 72.22%, 80.85%, and contrast medicament 50% Sukeling WP1000 is 81.96% to the average preventive effect of grey mould fruit rot of strawberry doubly.Preventive effect through arcsine conversion back with the check of DMRT method, on 5% significance level, except that handle between 30 times and contrast medicament and 50 times and 100 times difference remarkable, it is remarkable that all the other respectively handle differences.
2.2 indoor antibacterial test part (result of the test see Table 2 and table 3)
After cultivating 48h, 10 times, 20 times, 50 times, 100 times, 200 times inhibition growth rates to grey mould fruit rot of strawberry of plant insecticide agent formulation are respectively 100%, 100%, 80.65%, 62.83% and 43.78%, other of plant insecticide agent formulation handled equal unrestraint effect, contrast medicament 50% Sukeling WP1000 is that upright 1000 times and the 2000 times inhibition growth rates to the grey mould fruit rot of strawberry bacterium of agent of cutting off the water of 74.34% and 68.57%, 12% bacterium are respectively 85.84% and 79.19% with 2000 times of inhibition growth rates to the grey mould fruit rot of strawberry bacterium doubly; Cultivate after 72 hours, 10 times, 20 times, 50 times, 100 times, 200 times inhibition growth rates to grey mould fruit rot of strawberry of plant insecticide agent formulation are respectively 100%, 100%, 85.83%, 71%, 55.96%, 500 times, 1000 times of plant insecticide agent formulations and 1500 times of then unrestraint effects, contrast medicament 50% Sukeling WP1000 is that upright 1000 times and the 2000 times inhibition growth rates to the grey mould fruit rot of strawberry bacterium of agent of cutting off the water of 83.37% and 81.13%, 12% bacterium are respectively 91.68% and 88.08% with 2000 times of inhibition growth rates to the grey mould fruit rot of strawberry bacterium doubly.
Table 1 field trial
| Handle | Repeat | Disease refers to | Preventive effect % |
| 200 times of plant insecticide agent formulations | 1 | 13.76 | 42.95 |
| 2 | 11.67 | 51.62 | |
| 3 | 11.28 | 53.23 | |
| 4 | 11.33 | 53.07 | |
| Mean value | 12.01 | 50.22 | |
| 100 times of plant insecticide agent formulations | 1 | 6.89 | 71.42 |
| 2 | 8.65 | 64.14 | |
| 3 | 9.02 | 62.06 | |
| 4 | 7.42 | 69.24 | |
| Mean value | 8.00 | 66.85 | |
| 50 times of plant insecticide agent formulations | 1 | 5.92 | 75.45 |
| 2 | 6.96 | 71.14 | |
| 3 | 7.34 | 69.57 | |
| 4 | 6.62 | 72.55 | |
| Mean value | 6.71 | 72.22 | |
| 30 times of plant insecticide agent formulations | 1 | 4.12 | 82.92 |
| 2 | 5.03 | 79.15 | |
| 3 | 4.47 | 81.48 | |
| 4 | 4.86 | 79.85 | |
| Mean value | 4.62 | 80.85 | |
| 50% Sukeling WP1000 doubly | 1 | 3.40 | 84.12 |
| 2 | 5.93 | 75.41 | |
| 3 | 3.76 | 84.41 | |
| 4 | 3.88 | 83.91 | |
| Mean value | 4.24 | 81.96 |
| CK | 1 | 21.42 | -- |
| 2 | 21.54 | -- | |
| 3 | 28.02 | -- | |
| 4 | 25.51 | -- | |
| Mean value | 24.12 |
Table 2: cultivate the colony diameter (mm) after 48 hours
Table 3: cultivate the colony diameter (mm) after 72 hours
3. conclusions and recommendations
Field test results can find out, multiple diseases such as plant insecticide agent formulation control grey mould fruit rot of strawberry, powdery mildew, eqpidemic disease have certain effect, except that 30 times with the contrast pharmacy control efficacy quite, other all is lower than the contrast medicament; Indoor antibacterial test is the result show, the inhibition growth rate that the concentration after 100 times is handled the grey mould fruit rot of strawberry bacterium all is starkly lower than contrast medicament 50% Sukeling WP and the upright agent of cutting off the water of 12% bacterium.The plant insecticide agent formulation has the advantage of nontoxic residue-free, can be used for nuisanceless fruit production.
Three, carried out the test of control vegetables, fruit tree fungal disease, virus disease and aphid, respond well.
Claims (6)
1. the preparation method of a plant insecticide disease-prevention growth promoter, it is characterized in that by weight primary crude drug being mixed, main primary crude drug is 1 part of golden cypress, 0.25～3.5 part on tobacco leaf, 0.25～4 part of kuh-seng, 0.15～2 part of azedarach, the water that per 1 part of primary crude drug adding is 8～12 parts decocts 2～3 times, each 45～70 minutes, collect simultaneously volatile oil and soup, with medical filtration, to be concentrated into density be 1.05～1.3, obtains plant insecticide disease-prevention growth promoter liquor.
2. the preparation method of plant insecticide disease-prevention growth promoter according to claim 1 is characterized in that also can adding in the primary crude drug 0.15～2 part of sweet wormwood.
3. the preparation method of plant insecticide disease-prevention growth promoter according to claim 1 is characterized in that also can adding in the primary crude drug 0.15～1.5 part of mother chrysanthemum.
4. the preparation method of plant insecticide disease-prevention growth promoter according to claim 2 is characterized in that also can adding in the primary crude drug 0.15～1.5 part of mother chrysanthemum.
5. according to claim 1 or 2 or the preparation method of 3 or 4 described plant insecticide disease-prevention growth promoters, it is characterized in that the plant insecticide disease-prevention growth promoter liquor that obtains adds 0.2～0.4% aromatic, 2～4% preservatives, 3～6% synergist by weight proportion and promptly gets plant insecticide disease-prevention growth promoter paste.
6. according to claim 1 or 2 or the preparation method of 3 or 4 described plant insecticide disease-prevention growth promoters, it is characterized in that the plant insecticide disease-prevention growth promoter liquor that obtains is spray dried to extract powder with spray drying tower, add 8% sodium bicarbonate, 10% fumaric acid fine powder by weight proportion, mix promptly getting plant insecticide disease-prevention growth promoter pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410022134XA CN1281136C (en) | 2004-03-26 | 2004-03-26 | Pesticidal disease-preventing plant growth promoter and its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410022134XA CN1281136C (en) | 2004-03-26 | 2004-03-26 | Pesticidal disease-preventing plant growth promoter and its prepn |
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| CN102696699A (en) * | 2012-06-07 | 2012-10-03 | 海南大学 | Murraya paniculata (L.) total flavones soluble powder and preparation method thereof |
| CN102986749B (en) * | 2012-12-26 | 2014-03-05 | 李香侠 | Plant protecting agent and preparation method thereof |
| CN103814966B (en) * | 2014-03-05 | 2015-09-02 | 菏泽学院 | A kind of Peony growing special-purpose plaster preventing and treating fungal disease and preparation method thereof |
| CN104938540B (en) * | 2015-07-17 | 2017-12-29 | 陈玉红 | A kind of shoe cover of outdoor exercises for mosquito repellent |
| CN104957192B (en) * | 2015-07-17 | 2018-02-27 | 泉州立唯体育场地设施有限公司 | A kind of shoe cover of field work for mosquito repellent |
| CN105707128B (en) * | 2016-03-09 | 2018-10-02 | 高小文 | A kind of composition pesticide and preparation method and application containing tea saponin |
| CN107302917A (en) * | 2016-04-21 | 2017-10-31 | 上海新舒环环保科技有限公司 | It is a kind of to prevent and treat the preparation method that pythium spp realizes soil remediation |
| CN105994422A (en) * | 2016-06-17 | 2016-10-12 | 枞阳县白云生态园林有限责任公司 | Olive leaf and grape botanical pesticide |
| CN108575671A (en) * | 2018-04-11 | 2018-09-28 | 合肥卓畅农业科技有限公司 | A kind of insect prevention organic nutrient soil of safety and environmental protection |
| CN110402741A (en) * | 2019-07-31 | 2019-11-05 | 广西澳一澳农业科技有限公司 | A kind of high yield macadamia implantation methods |
| CN113951285B (en) * | 2021-10-13 | 2023-05-30 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
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