CN1662556B - 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法 - Google Patents

在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法 Download PDF

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Publication number
CN1662556B
CN1662556B CN038146266A CN03814626A CN1662556B CN 1662556 B CN1662556 B CN 1662556B CN 038146266 A CN038146266 A CN 038146266A CN 03814626 A CN03814626 A CN 03814626A CN 1662556 B CN1662556 B CN 1662556B
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framework
antibody
sequence
scfv
yeast
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CN1662556A (zh
Inventor
K·缇索特
S·艾沃特
A·奥弗德尔茂尔
A·巴伯里斯
D·艾施尔
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Novartis AG
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Asba Technology Ag
Esbatech An Alcon Biomedical Research Unit LLC
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Priority to CN201410031953.4A priority patent/CN103739706B/zh
Publication of CN1662556A publication Critical patent/CN1662556A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C07K2317/82—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies functional in the cytoplasm, the inner aspect of the cell membrane, the nucleus or the mitochondria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN038146266A 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法 Expired - Lifetime CN1662556B (zh)

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CN201510685456.0A CN105175535A (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201410031953.4A CN103739706B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法

Applications Claiming Priority (5)

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US38264902P 2002-05-22 2002-05-22
US60/382,649 2002-05-22
US43825603P 2003-01-03 2003-01-03
US60/438,256 2003-01-03
PCT/EP2003/005324 WO2003097697A2 (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

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CN201410031953.4A Division CN103739706B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201010575786.1A Division CN102093477B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201510685456.0A Division CN105175535A (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法

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CN1662556A CN1662556A (zh) 2005-08-31
CN1662556B true CN1662556B (zh) 2013-05-01

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CN038146266A Expired - Lifetime CN1662556B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201510685456.0A Pending CN105175535A (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201410031953.4A Expired - Lifetime CN103739706B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201010575786.1A Expired - Lifetime CN102093477B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法

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CN201410031953.4A Expired - Lifetime CN103739706B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法
CN201010575786.1A Expired - Lifetime CN102093477B (zh) 2002-05-22 2003-05-21 在胞内环境中表现出增强的稳定性的免疫球蛋白构架及其鉴定方法

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US (5) US8853362B2 (https=)
EP (5) EP2314622B1 (https=)
JP (10) JP2006508638A (https=)
CN (4) CN1662556B (https=)
AU (2) AU2003238370B2 (https=)
CA (2) CA2483285C (https=)
CY (1) CY1114225T1 (https=)
DK (2) DK1506236T3 (https=)
ES (3) ES2399617T3 (https=)
HU (1) HUE048922T2 (https=)
NZ (1) NZ536412A (https=)
PT (2) PT2332989E (https=)
SI (2) SI2332989T1 (https=)
WO (1) WO2003097697A2 (https=)

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图1、6.

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JP2006508638A (ja) 2006-03-16
JP2013078343A (ja) 2013-05-02
US20150057191A1 (en) 2015-02-26
HK1217712A1 (en) 2017-01-20
DK1506236T3 (da) 2013-03-04
HUE048922T2 (hu) 2020-09-28
JP2019058191A (ja) 2019-04-18
AU2010202634B2 (en) 2012-08-23
ES2399617T3 (es) 2013-04-02
EP3656787A1 (en) 2020-05-27
JP2020171286A (ja) 2020-10-22
EP2314622B1 (en) 2017-10-18
AU2010202634A1 (en) 2010-07-15
US20200165322A1 (en) 2020-05-28
PT2332989E (pt) 2015-08-05
CN105175535A (zh) 2015-12-23
EP2947095A1 (en) 2015-11-25
AU2003238370A1 (en) 2003-12-02
HK1071378A1 (en) 2005-07-15
CA2867542A1 (en) 2003-11-27
HK1152320A1 (en) 2012-02-24
ES2537104T3 (es) 2015-06-02
DK2332989T3 (en) 2015-05-11
EP2314622A1 (en) 2011-04-27
CA2483285A1 (en) 2003-11-27
CA2483285C (en) 2015-01-27
CN102093477B (zh) 2014-02-26
EP2947095B1 (en) 2019-10-02
JP6261136B2 (ja) 2018-01-17
CY1114225T1 (el) 2016-08-31
CN102093477A (zh) 2011-06-15
JP2015214583A (ja) 2015-12-03
SI2947095T1 (sl) 2020-04-30
US9518108B2 (en) 2016-12-13
PT1506236E (pt) 2013-03-27
JP6075836B2 (ja) 2017-02-08
EP2332989A1 (en) 2011-06-15
JP6100544B2 (ja) 2017-03-22
US20190119361A1 (en) 2019-04-25
CN103739706B (zh) 2015-11-18
JP2010187678A (ja) 2010-09-02
EP1506236B1 (en) 2013-01-23
SI2332989T1 (sl) 2015-06-30
JP2012228272A (ja) 2012-11-22
CA2867542C (en) 2020-04-14
JP2017201990A (ja) 2017-11-16
CN1662556A (zh) 2005-08-31
US8853362B2 (en) 2014-10-07
JP2013256533A (ja) 2013-12-26
WO2003097697A2 (en) 2003-11-27
EP1506236A2 (en) 2005-02-16
EP2332989B1 (en) 2015-04-01
JP6918033B2 (ja) 2021-08-11
JP2015227372A (ja) 2015-12-17
US20170107272A1 (en) 2017-04-20
US20060035320A1 (en) 2006-02-16
AU2003238370B2 (en) 2010-04-29
CN103739706A (zh) 2014-04-23
WO2003097697A3 (en) 2004-12-09
US10125186B2 (en) 2018-11-13
US10570190B2 (en) 2020-02-25
ES2656427T3 (es) 2018-02-27

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