CN1642431A - Sterilization methods, sterilizing/washing agent and washing method - Google Patents

Abstract

A sterilization method characterized by comprising treating a material having bacterial cells adhering thereto with an aqueous solution which contains a lysokinase tolerant to chelating agents at such a concentration as giving a lytic activity of 2.5 U/mL or above, has a calcium ion concentration (in terms of CaO) of 10 ppm or below and has a pH value exceeding 8 but not higher than 12. A sterilization method characterized by comprising treating a material having bacterial cells adhering thereto with an aqueous solution which contains a lysokinase tolerant to chelating agents at such a concentration as giving a lytic activity of 15 U/mL or above and has a pH value exceeding 8 but not higher than 12.

Description

Sterilization method, sterilization/washing agent, and washing methods

Technical field

The present invention relates to sterilization method, sterilization de-sludging composition, and washing methods, they all are designed to by handling with lysozyme the material of germ contamination effectively be sterilized.

Background technology

To the conventional method that the material of germ contamination carries out disinfection, comprise the method (Japan Patent the spy open: clear 63-131124,2000-26894, clear 63-286158 peace 11-29797 etc.) that depends on bactericide or bleaching agent.

Proposed to be used for the disinfectant of the employing enzymatic reaction of contact lenses already.It comprises the enzyme that acts on microorganism wall, acts on the enzyme of cell membrane, and (Japan Patent the spy open: flat 9-10288) for antibacterium albumen and antifungal drug.

Unfortunately, above-mentioned conventional method has the defective that needs strong and stimulating bactericide or special compound.Therefore, exist the new method of effectively sterilizing and the needs of compound with weak excitant composition.In addition, above-mentioned conventional method is designed to be applied to bacterium alive.But, because killed bacterial has caused the smell in kitchen and bathroom usually and has been clamminess and this fact of peculiar smell of textile, exist can effectively decomposing and remove the method for killed bacterial and the needs of compound

Summary of the invention

The purpose of this invention is to provide sterilization method, sterilization de-sludging composition, and washing methods, they all are designed to bacterium is decomposed and remove by low stimulation component.

The inventor finds, if handle the material that is subjected to germ contamination with a kind of aqueous solution that contains the lysozyme of specific concentrations, can effectively decompose and remove dead bacterium, described endonuclease capable tolerance chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity to have specified level, the calcium ion that also contains certain content, and the pH value is higher than 8, but be no more than 12.This discovery has caused the present invention.

The present invention relates to sterilization method, disinfecting cleaning composition and the method that is used to wash textile will be described in more detail it below.

(1) a kind of sterilization method comprises

Handle the material that is subjected to germ contamination with a kind of aqueous solution, this solution contains the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contain the calcium ion that concentration is no more than the CaO form of 10ppm, the pH value is higher than 8, but is no more than 12.

(2) a kind of sterilization method comprises

Handle the material that is subjected to germ contamination with a kind of aqueous solution, this solution contains the lysozyme that can tolerate chelating agent, and the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12.

(3) a kind of not phosphatic disinfecting cleaning composition, under its common form or its aqueous solution form, contain the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contain the calcium ion that concentration is no more than the CaO form of 10ppm, the pH value is higher than 8, but is no more than 12.

(4) a kind of not phosphatic disinfecting cleaning composition, under its common form or its aqueous solution form, contain the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12.

(5) the not phosphatic method that contains the disinfecting cleaning composition washing textile of the lysozyme that is subjected to chelating agent of having the capability of doing sth of a kind of usefulness, described method comprises with a kind of solution washing textile, so that sterilization, described solution prepares with described disinfecting cleaning composition, so that

(a) it contains the lysozyme that can tolerate chelating agent, and described enzyme is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contains the calcium ion that concentration is no more than the CaO form of 10ppm, and the pH value is higher than 8, but is no more than 12, or:

(b) it contains the lysozyme that can tolerate chelating agent, and described enzyme is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12,

Thereby avoid during indoor seasoning or after drying storage period, textile gives off a peculiar smell.

Implement best mode of the present invention

In the present invention, term " can tolerate the lysozyme of chelating agent " and is and can have any lysozyme that keeps its bacteriolyze activity under the situation of chelating agent.Specifically, it is to keep its bacteriolyze activity to be not less than 50%, preferably be not less than 80% enzyme, described activity is defined as (U/U ') * 100, wherein, U is illustrated in the active unit that measures under the situation that has chelating agent (EDTA-4Na), and U ' is illustrated in the active unit that measures under the do not have chelating agent situation of (EDTA-4Na), and carry out by " method of analytical unit (U) " by mentioning below for mensuration.Preferred lysozyme is the protease with bacteriolyze activity.The unit definition of bacteriolyze activity is as follows.

The method of＜analytical unit (U) 〉

By under 121 ℃ temperature, adding hot distilled water 20 minutes, prepare sterile water.With EDTA-4Na (111ppm), sodium bicarbonate (278ppm) and described enzyme are dissolved in this sterile water.With sodium hydroxide this solution is adjusted to PH8.5, from this solution, take out the amount of 9mL, and in 25 ℃ water-bath, be incubated 5 minutes.Add the liquid that 1mL contains the Escherichia coli ATCC8739 of heated and inactivated in this sample solution, it is about 3.3 that colibacillary content makes the turbidity of solution, and turbidity is to measure under the wavelength of 660nm with spectrophotometer.Within back 30 seconds of stirring, the turbidity of this solution is 0.3 ± 0.03, and this turbidity is to measure under the wavelength of 660nm with spectrophotometer.Resulting solution is called as " U test solution ".This U test solution is incubated 10 minutes in 25 ℃ water-bath.After the stirring, under the wavelength of 660nm, measure the turbidity of this solution.The amount that can make the turbidity of U test solution reduce by 0.05 needed protease in 10 minutes is defined as 10 units.By the way, the bacteriolyze rate is to measure by following method.

＜be used to measure the method for bacteriolyze rate 〉

(preparation of bacteria tested solution)

(37g/L) heart and brain lixiviate media (available from Difco) of ormal weight are dissolved in the water of purifying.In the Skaguchi of 500mL flask, resulting solution (50ml) is carried out disinfection.Inoculate described sterile solution with the platinum loop of an Escherichia coli ATCC8739.The medium of inoculating is rocked cultivation 18 hours under 37 ℃.Collected bacterial cell in 5 minutes by speed (approximately 1800G) centrifugation with 3000rpm.The bacterial cell of collecting is suspended in the sterile water, and carries out once more centrifugal suspension.The bacterial cell of collecting is suspended in the sterile water.Heated 20 minutes down at 90 ℃, then the cell that heated by centrifugal collection.The bacterial cell of collecting is suspended in the sterile water, makes that the turbidity of suspension is 3.3, this turbidity is to measure under the wavelength of 660nm.

(measuring the bacteriolyze rate)

To carry out the sample solution (9ml) of bacteriolyze rate mensuration and put into test tube.After in 25 ℃ water-bath, being incubated 5 minutes, described sample solution is mixed with the 1ml bacterial cell suspension that obtains according to the method described above.Thereby obtained to be used to measure the solution (10ml) of bacteriolyze rate.Within back 30 seconds of reaction beginning, under the 660nm wavelength, measure the turbidity of this solution.In 25 ℃ water-bath, this solution is incubated 10 minutes.After the stirring, under the wavelength of 660nm, measure the turbidity of this solution.Replace described bacterial cell suspension with the sterile water of 1ml and carry out blank determination.By the way, described " being used to measure the solution (10ml) of bacteriolyze rate " is preparation like this: it is had and the described aqueous solution that is used to measure or the identical concentration of disinfecting cleaning composition (solution form).

The bacteriolyze rate is to obtain by following formula.

According to the present invention, the defined aqueous solution in top (1), defined disinfecting cleaning composition (primitive form or dilute with water are crossed) in top (3), and defined disinfecting cleaning composition (it is called 2.5U/mL and above solution for short) in (a) of top (5), should contain is enough to make its bacteriolyze activity level to be not less than the lysozyme of the concentration of 2.5U/ml, preferred 3-400000U/mL, more preferably 15-400000U/mL.In addition, the defined aqueous solution in top (2), defined disinfecting cleaning composition (primitive form or dilute with water are crossed) in top (4), and (b) defined disinfecting cleaning composition in top (5) (it is called 15U/mL and above solution for short), should contain is enough to make its bacteriolyze activity level to be not less than the lysozyme of the concentration of 15U/ml, preferably be not less than 16U/mL, more preferably 20-400000U/mL.In other words, should the concentration of lysozyme in the described solution suitably be adjusted according to top regulation.Under the occasion that after water dilutes disinfecting cleaning composition, is used to wash, the upper limit of concentration of described lysozyme should be preferably such: for the solution of 2.5U/mL, the bacteriolyze activity is 3000U/mL, and for 15U/mL and above solution, the bacteriolyze activity is 1000U/mL.By the way, the bacteriolyze activity (U/mL) of solution of the present invention is by the bacteriolyze activity (U/ Unit Weight) according to described enzyme, is obtained by the concentration conversion of each enzyme in this solution.By the way, 2.5U/mL and above solution and 15U/mL and above solution are represented the aqueous solution or the disinfecting cleaning composition of solution form uniformly and simply.

The exemplary of lysozyme comprises following enzyme.Spill Wei Naisi 12T, spill Wei Naisi 16L, Esperase 4T, Everlase 8T, Kannase 24TK, Alcalase 2T (all available from Novozymes company).Respectively by bacillus P, bacillus K, the P-enzyme that bacillus X and bacillus Y produce, the K-enzyme, X-enzyme and Y-enzyme, described bacterium is kept at fermentation research institute, and preserving number is respectively 8090,8091, and 8092 and 8088.In the above-mentioned example, preferably spill Wei Naisi 12T, spill Wei Naisi 16L, Esperase 4T, Everlase 8T, Kannase24TK, Alcalase 2T, Deozyme and Y enzyme.Described lysozyme with any other enzyme combination such as lipase after can produce the bacteriolyze effect of enhancing, produce good detergency thus.

The activity of lipase changes, and its activity is normally determined via the amount of measuring the fatty acid that it discharges by decomposition from substrate.Can mix the lipase with enzymic activity in cleaning compositions of the present invention, so that when substrate is decomposed, the amount of the fatty acid that discharges from triolein is not less than 2 times of amount (calculating with molal quantity) of the fatty acid that discharges from n-cetyl palmitate.The activity of described lipase is to determine by the decomposition of measuring substrate in accordance with the following methods.

The base soln of preparation deionized water, it contains the sodium salt of the alpha-sulfo fatty acid methyl ester of 200ppm, and (α-SF-Na), (in CaO) calcium chloride of the sodium carbonate of 200ppm and 30ppm is so that adjust to 3 ° of DH with Deutschland hardness.α-SF-Na is to be the C of 2: 8 (weight ratio) by ratio ₁₄And C ₁₆Fatty acid is formed.Temperature is remained on 22.5 ± 0.5 ℃ described base soln (in 100 ± 0.2mL) beakers of having put into magnetic force splash bar (diameter 8mm, length 30mm) (internal diameter 63mm, height 80mm).In described base soln, provide 0.2g triolein (available from the preparation of Fulka) or n-cetyl palmitate (available from the preparation of Tokyo Kasei Kogyo) to make substrate.After stirring 15-20 minute, the pH of this solution becomes stable (approximately 10-10.5).This pH value is called as initial pH value.Stirring is by using titration outfit (TS-980 is available from Hiranum Sangyo), realizes with the speed of 200rpm.The pH value is by being connected pH determination of electrode on the described titration outfit.

By continuous stirring at room temperature, in this solution, drip the KOH solution of 0.1N, so that initial PH is remained on about 10-10.5.After beginning to drip 1 minute, add the lipase sample, so that the concentration of lipase in described base soln remains in 0.1-0.01ppm (finger protein matter) scope.Measure the amount of the 0.1N KOH solution that had dripped 61 minutes already.For triolein and n-cetyl palmitate, the concentration of lipase should be identical.

The amount of the KOH solution of dropping is regarded as the amount of the fatty acid that discharges from substrate (it is triolein or n-cetyl palmitate) after adding lipase.Repeat above process 10 times, and mean value is used for calculating described enzymic activity (it is the mol ratio of the amount of the amount of the fatty acid that discharges from triolein and the fatty acid that discharges) from n-cetyl palmitate.

Example with lipase of above-mentioned enzymic activity is the enzyme (being disclosed in the patent No.: in the PCT translator of Japanese part of flat 11-510699) that comes from the pubescence humicola lanuginosa.This kind of enzyme can be a trade mark with " Lipex ", buys from Novozymes company.

The content of lipase is preferably the 0.01-5wt%, particularly 0.1-2wt% (lipase preparation) of described aqueous solution gross weight or described disinfecting cleaning composition (solution form).

Described lipase can replace with amylolytic enzyme, as opening the Japan Patent spy: the enzyme of the best pH meta-alkalescence that discloses among the flat 2-49584; At Japanese PCT application number: the optimum temperature that discloses among the 2001-521739 is no more than 60 ℃ enzyme; At Japanese PCT application number: what disclose among the flat 11-50300 has the enzyme of tolerance to calcium; As opening the Japan Patent spy: flat 3-87176, flat 3-87177, the α-1,6 of the energy cracking starch that discloses among the flat 3-108482 peace 6-14775 connects the enzyme of key.

Another preferred amylase is the wild type amylase that comes from the bacillus that passes through the sudden change modified.

Diastatic example comprises Duramyl 60T (available from Novozymes) and amylopectase (available from Sigma).They can use separately or combination with one another is used.

Diastatic content is preferably the 0.01-5wt%, particularly 0.1-2wt% (referring to enzyme preparation) of described aqueous solution gross weight or described disinfecting cleaning composition (solution form).

Disinfecting cleaning composition of the present invention is substantially free of phosphate.But it can contain the carbamate additives for low phosphorus hydrochlorate (being no more than 0.5wt%) that comes from enzyme preparation.

Have 2.5U/mL and above solution, should contain concentration and be no more than 10ppm, preferred 0.01-9ppm, the more preferably calcium ion of 0.01-5ppm (referring to CaO).Have 15U/mL and above solution, should contain concentration and be no more than 10ppm, preferred 0.01-20ppm, more preferably 0.01-15ppm, the especially preferably calcium ion (referring to CaO) of 0.01-10ppm.The existence of the calcium ion in the above-mentioned concentration range has improved the effect of described lysozyme.

By the way, the concentration of described calcium ion (referring to CaO) is to open according to being disclosed in the Japan Patent spy: the method among the embodiment of flat 9-318615 (adopting the absorptionmetry of pontachrome colorss purple) is measured.

Calcium ion concentration in the above-mentioned concentration range is by the described aqueous solution or disinfecting cleaning composition and one or more special chelating agent (effective especially for calcium ion) and general chelating agent are combined acquisition.

The example of described special chelating agent comprises the derivative of amino carboxylic acid, polycarboxylic acids, and the chelating agent of the form of its salt.

The example of the chelating agent of the derivative form of amino carboxylic acid comprises amino methyl glycine diacetate esters, the Beta-alanine diacetate esters, the imino group succinate, oxyimino succinate (HIDS), serine diacetate esters, the isoerine diacetate esters, aspartic acid diacetate esters (ASDS), ethylenediamine tetra-acetic acid (EDTA), nitrilotriacetate (NTA), HEDTA ester (HEDTA) and inferior second pentaacetic acid (DTPA).Other examples comprise EGTA (ethylene glycol bisthioglycolate [the amino ether of 2-] tetracetate).Described chelating agent should be preferably sodium salt or potassium salt form.

If the described aqueous solution or disinfecting cleaning composition (solution form) are used for textile, described chelating agent should be preferably below any one of chelating agent: amino methyl glycine diacetate esters, the Beta-alanine diacetate esters, the imino group succinate, oxyimino succinate (HIDS), serine diacetate esters, isoerine diacetate esters and aspartic acid diacetate esters (ASDS).

Polycarboxylic acids, and the example of the form of its salt comprises polymer dielectric, as polyacrylic acid, the polycarboxylate of maleic acid-acrylic acid copolymer and polysaccharide.The mean molecule quantity of described polyacrylic acid or maleic acid-acrylic acid copolymer is 1000-1000000, preferred 1000-100000.The salt of polycarboxylic acids is sodium salt or potassium salt form preferably.

As polycarboxylic acids, or the polycarboxylate of the polysaccharide of a kind of example of its salt, be the product that reacts and obtain by polysaccharide and oxidant (as hypochlorite, periodate, and chlorite), described reaction can cause cracking, to discharge hydroxy-acid group.The example of polysaccharide comprises starch, dextrin, cellulose, hemicellulose, amylose and amylopectin.They should preferably contain the DEXTROSE ANHYDROUS unit of oxidation, and its content is 50-100mol%, preferred 70-95mol%.In these examples, the starch of oxidation (being obtained by starch by oxicracking) is most preferred.

The example of general chelating agent comprises crystal aluminosilicate (zeolite A, P, and X), the non-crystalline silicon aluminate, crystalline alkali metal silicate (having layer structure), the chelating agent of low-molecular-weight hydroxyl carboxylic acid form (citric acid and salt thereof, gluconic acid and salt thereof, glycolic and salt thereof, with maleic acid and salt thereof), and the chelating agent of phosphonic acids form (as 1-hydroxyl ethane-1,1-di 2 ethylhexyl phosphonic acid (HEDP), and inorganic phosphate (as tripolyphosphate and pyrophosphate) and ATMP (ATMP)).

In the cited in the above chelating agent, without phosphorus chelating agent is particularly preferred.

The pH value of the described aqueous solution or disinfecting cleaning composition (solution form), for having 2.5U/mL and above solution, should be higher than 8, but be no more than 12, preferred 8.1-11 has 15U/mL and above solution, pH value should be higher than 8, but be no more than 12, preferred 8.1-11, preferred especially 9-11.It is necessary that this PH scope is that lysozyme is given full play to its bacteriolyze effect.PH value is high more, and the bacteriolyze effect is good more.

Disinfecting cleaning composition of the present invention can be used as the textile washing agent, washes one's hands and dishwashing detergent bathroom washing agent, washing machine washing agent, and toilet and drainpipe washing agent.

Be used as at described disinfecting cleaning composition under the occasion of laundry detergent compositions, the lysozyme content in the described composition should be preferably 1000-400000U/g, particularly 4500-60000U/g, more preferably 30000-60000U/g.The pH value of the described aqueous solution or disinfecting cleaning composition (solution form) should be preferably 8.1-11, preferred especially 8.5-11.

Be used as at described disinfecting cleaning composition under the occasion of dishwashing detergent, the lysozyme content in the described composition should be preferably 100-400000U/g, preferred especially 300-20000U/g.The pH value of the aqueous solution or disinfecting cleaning composition (solution form) should be 8.1-10, preferred 8.1-9.

Be used as at described disinfecting cleaning composition under the occasion of bathroom washing agent, the lysozyme content in the described composition should be preferably 1000-400000U/g, preferred especially 2000-40000U/g.The pH value of the described aqueous solution or disinfecting cleaning composition (solution form) should be 8.1-10, preferred especially 8.1-9.

Be used as at described disinfecting cleaning composition under the occasion of washing machine washing agent, the lysozyme content in the described composition should be preferably 1000-100000U/g, preferred especially 2000-20000U/g.The pH value of the described aqueous solution or disinfecting cleaning composition (solution form) should be preferably 8.1-12, preferred especially 8.5-9.

Be used as at described disinfecting cleaning composition under the occasion of toilet and drainpipe washing agent, the lysozyme content in the described composition should be preferably 1000-100000U/g, preferred especially 2000-20000U/g.The pH value of the described aqueous solution or disinfecting cleaning composition (solution form) should be preferably 9-12, preferred especially 9-11.

Disinfecting cleaning composition of the present invention should be preferably and surface-active preparation (surfactant) combination, and it can cause described chelating agent effectively to be penetrated in the spot, thereby promote bacteriolysis.

The example of surfactant comprises anion surfactant, non-ionic surface active agent, cationic surfactant, amphoteric surfactant.They can use separately, or combination with one another is used.

The content of described surfactant should preferably account for the 3-50wt% of described disinfecting cleaning composition total amount, more preferably 5-40wt%.Anion surfactant or non-ionic surface active agent should preferably be used as main surfactant, and it accounts for the largest portion of employed surfactant total amount.

Examples of anionic surfactants comprises straight chain (C _10-14) alkylbenzenesulfonate, the rudimentary (C of alpha-sulfo-fatty acid _1-3) alkyl ester salt, (C _10-20) the ester salt of alkyl sulfate, alkene sulfonate, alkane sulfonate, the ester salt of polyxyethylated (or thiazolinyl) sulfuric ester, and saturated or unsaturated (C _12-22) fatty acid.Described salt can be alkali metal salt (sodium salt and sylvite), amine salt, or ammonium salts.

The content of described anion surfactant should preferably account for the 5-45wt% of described disinfecting cleaning composition total amount, more preferably 10-35wt%.

The preferred example of non-ionic surface active agent is as follows.

(1) add the non-ionic surface active agent (alkyl ether ethoxylate) of EO, it by 1 mole alcohol (par of carbon is 10-20) and the oxirane (EO) of the 5-30 mole (mean) inside adding to form.

(2) add the non-ionic surface active agent of EO-PO, it by 1 mole alcohol (par of carbon is 10-20) and the oxirane (EO) and the expoxy propane (PO) of the 5-30 mole (mean) inside adding to form.

(3) by the non-ionic surface active agent of the fatty acid ester type of following structural formula (1) expression.

R ¹-CO(OR ²) _nOR ³??????????????????(1)

(wherein, R ¹Be C _5-21(preferred C _9-17) the alkyl or alkenyl group; OR ²Be the C that is added _2-4The unit of alkylene oxide, n are the OR that is added ²Average mol (it is 5-30, preferred 5-20), and R3 is C _1-4(preferred C _1-2) alkyl group.Described alkylene oxide hydrocarbon should be preferably the combination of EO itself or EO and PO).

Non-ionic surface active agent should preferably account for the 1-40wt% of described disinfecting cleaning composition total amount, more preferably 1-30wt%.

The preferred example of zwitterionic surfactant comprises betain (as lauric acid aminopropyl betain and stearic acid amino-ethyl betain), and imidazolidine derivatives (as N-coco-nut oil fatty acid acyl group-N-carboxyethyl-N-hydroxyethyl-ethylenediamine sodium).

Be used as clothing at described disinfecting cleaning composition, wash one's hands and wash the dishes, when bathroom and washing machine washing agent, should preferably contain surfactant (particularly anion surfactant).

When described disinfecting cleaning composition was used as laundry detergent compositions, described anion surfactant is linear alkylbenzene sulfonate (LAS) preferably, the alkyl ester salt of alpha-sulfo-fatty acid, alkene sulfonate, alkane sulfonate, alkyl sulfate, and soap.In described surfactant, the alkyl ester salt of preferred alpha-sulfo-fatty acid is because it just has good detergency when a small amount of the use.

Be used as at described disinfecting cleaning composition and wash one's hands and during dishwashing detergent, described anion surfactant should be preferably polyxyethylated or the ester salt of thiazolinyl sulfuric ester, linear alkylbenzene sulfonate (LAS), the alkyl ester salt of alpha-sulfo-fatty acid, alkene sulfonate, alkane sulfonate, alkyl sulfate, and soap.

By the way, the concentration of described surfactant in described solution or disinfecting cleaning composition (solution form) should be preferably: as washing the dishes and during the tank washing agent, 1-20wt%, as toilet, drainpipe and bathroom (bath tube) washing agent the time, 0.5-1.5wt%, as the washing machine washing agent time, 0.05-0.5wt%, and as the textile washing agent time, 100-1200ppm.

Disinfecting cleaning composition of the present invention can directly use, and perhaps uses with the dilute aqueous form.Under latter event, the concentration of described cleaning compositions should be preferably 0.04-10wt%, more preferably 0.04-8wt%, especially preferably 0.05-1wt%.Can determine suitable concentration according to the purposes of cleaning compositions.

The concentration of described disinfecting cleaning composition in its aqueous solution is as follows.1-50wt% is used for washing the dishes at tank or wash bowl, and 5-50wt% is used for washing the dishes with sponge.1000-100000ppm is used for the bathroom washing.1000-10000ppm is used for washing machine, and 3-100wt%, particularly 5-50wt% are used for toilet and drainpipe washing.

Disinfecting cleaning composition of the present invention can make up with various compositions, and its consumption can not destroy purpose of the present invention, and described composition is added to usually and is used to wash the dishes, the bathroom, and tank, drainpipe is in the washing agent of toilet and laundry.

Disinfecting cleaning composition of the present invention can prepare with various forms, as liquid, and gel, spray, powder, tablet, agglomerate, sheet material and bar.

Disinfecting cleaning composition of the present invention is not confined to described preparation method especially; It can be by being usually used in preparing the common methods preparation of common washing agent.

Disinfecting cleaning composition of the present invention is especially suitable for use as laundry detergent compositions.It can be sterilized and cleaning textiles, and can prevent textile during indoor seasoning, and after the drying, or storage period gives off a peculiar smell.It is specially adapted to washing at the textile of indoor seasoning and sterilize.In addition, it can decompose and sterilize the bacterium of death.

By the way, described cleaning compositions can it packing or brochure on record above-mentioned functions and effect so that propagate by means of symbol or chart.

Disinfecting cleaning composition of the present invention can effectively decompose and remove dead bacterium.Washing methods of the present invention can prevent clothing during indoor seasoning, and after the drying, or storage period gives off a peculiar smell.

The feature of the top relevant disinfecting cleaning composition of mentioning is equally applicable to be used to each composition and the aqueous solution of sterilization method of the present invention and washing methods.

In the present invention, term " material of germ contamination " refers to the crust of kitchen basin, dustbin, washbowl, drainpipe, bath tube, bathroom, and toilet seat, and textile comprise clothing, bedding, curtain, carpet, rug and cleaning brush etc.

Described sterilization method does not have particular restriction, and the method for optimizing of material that is used for germ contamination is as mentioned below.

When the material of described germ contamination is when having the permanent plant of crust and device, sterilization should be preferably by they being immersed in the described aqueous solution or with described aqueous solution spray or wash and realize.

When the material of described germ contamination was clothing and other textiles, sterilization should preferably realize by washing in washing machine (after soaking).

By the consumption and the temperature of the described aqueous solution of suitable adjustment, and the processing time, above-mentioned bacteriolyze effect can be strengthened.

For having 2.5U/mL and above solution, described temperature should be preferably 10-90 ℃, and particularly 10-60 ℃, and the processing time should be preferably 5 seconds-10 hours, particularly 10-60 minute.

For having 15U/ml and above solution, temperature is preferably 10-90 ℃, is preferably 10-60 ℃ especially, and the processing time was preferably 5 seconds-60 minutes, was preferably 5 seconds especially-30 minutes.

When the material of described germ contamination is that the consumption of the described aqueous solution should be preferably 0.01-0.5mL/cm when having the permanent plant of crust and device ²(surface area), particularly 0.1-0.3mL/cm ²For the textile of germ contamination, the consumption of the described aqueous solution should be preferably the 1-30mL/g fiber.

The invention provides disinfection treatment method, disinfecting cleaning composition, washing methods, they are designed to by weak excitant composition decomposition and remove bacterial cell.

Embodiment

Below in conjunction with following examples the present invention is done to illustrate in greater detail, these embodiment will limit scope of the present invention.In following examples, % represents wt%.

Embodiment 1-17 and comparing embodiment 1-5

Prepare the aqueous solution of the composition of table 1 shown in also with urban water, described urban water is adjusted to 3.3 ° of DH (CaO is 33mg/L) with two hydration calcium chloride with it.Measure the bacteriolyze rate of obtained aqueous solution in accordance with the following methods.The result is shown in table 1 and 2.By the way, the bacteriolyze activity of employed enzyme is measured by the following method.In table 1 and 2, the concentration of each enzyme is to represent with the U/mL form of the conversion of the bacteriolyze activity value (U/ Unit Weight) by described enzyme sample.

By the way, thermolysin (it be not the enzyme of anti-chelating agent) is to use with the form of dilute solution (10mg/L), in table 2 with asterisk (*) expression.

＜be used for the method for analytical unit (U) 〉

With EDTA-4Na (111ppm), sodium bicarbonate (278ppm) and enzyme are dissolved in and prepare the aqueous solution in the distilled water, and distilled water is to sterilize in 20 minutes by heating under 121 ℃ temperature.With sodium hydroxide this solution is adjusted to PH8.5, then, this solution of 9mL is incubated 5 minutes in 25 ℃ water-bath.Add the suspension that 1mL contains the Escherichia coli ATCC8739 of heating in this solution, the turbidity that makes this suspension is about 3.3, and turbidity is (more details is referring to following paragraph) measured under the wavelength of 660nm with spectrophotometer.Within back 30 seconds of stirring, the turbidity of this solution is 0.3 ± 0.03, and this turbidity is to measure under the wavelength of 660nm.Obtain " U test solution " like this.This U test solution is incubated 10 minutes in 25 ℃ water-bath.After the bacterium of adding described heating 10 minutes, stir described U test solution, and under the wavelength of 660nm, measure the turbidity of this solution.The amount that can make turbidity (under the wavelength of 660nm) reduce by 0.05 needed protease in 10 minutes is defined as 10 units (or 10U).

The bacteriolyze rate is to measure by following method.

＜be used to measure the method for bacteriolyze rate 〉

(preparation of bacteria tested solution)

(37g/L) heart and brain lixiviate media (available from Difco) of ormal weight are dissolved in the water of purifying.In the Sakaguchi of 500mL flask, resulting solution (50ml) is carried out disinfection.Inoculate described sterile solution with an Escherichia coli ATCC8739 platinum loop.The medium of inoculating is rocked cultivation 18 hours under 37 ℃.By with the speed of 3000rpm (approximately 1800G) centrifugation 5 minutes, collect bacterial cell.The bacterial cell of collecting is suspended in the sterile water, and carries out centrifugal with the speed (approximately 1800G) of 3000rpm to suspension once more.The bacterial cell of collecting is suspended in the sterile water.Heated 20 minutes down at 90 ℃, centrifugal by speed (approximately 1800G) then with 3000rpm, collect the cell that heated.The cell suspension collected in sterile water, is obtained turbidity and be 3.3 suspension, and this turbidity is to measure under the wavelength of 660nm.

(measuring the bacteriolyze rate)

To carry out the sample solution (9ml) of bacteriolyze rate mensuration and put into test tube.After in 25 ℃ water-bath, being incubated 5 minutes, described sample solution is mixed with the bacterial cell suspension that obtains according to the method described above.Thereby obtained to be used to measure the solution (10mL) of bacteriolyze rate.Within back 30 seconds of reaction beginning, under the 660nm wavelength, measure the turbidity of this solution.In 25 ℃ water-bath, this solution is incubated 10 minutes.After the stirring, under the wavelength of 660nm, measure the turbidity of this solution.Replace described bacterial cell suspension with the sterile water of 1mL and carry out blank determination.By the way, if be used to measure under the wavelength of turbidity at 660nm of sample before adding described bacterial cell suspension of bacteriolyze rate and surpassed 0.7, should filter with miillpore filter (0.45 μ m) in advance, and in resulting filtrate (9mL), add described bacterial cell suspension (1mL).By the way, described " being used to measure the solution (10mL) of bacteriolyze rate " is to prepare like this, and it is had and the described aqueous solution that is used to measure or the identical concentration of disinfecting cleaning composition (solution form).

The bacteriolyze rate is to obtain by following formula.

＜mensuration calcium concentration 〉

" being used to measure the solution of bacteriolyze rate " the tested solution of mentioning in the paragraph of superincumbent " measuring the bacteriolyze rate " (10mL) replaces, and wherein, has added the sterile water of 1mL, to replace " described bacterial cell suspension ".Open according to being disclosed in the Japan Patent spy: the method (adopting the absorptionmetry of pontachrome colorss purple) of the embodiment part of flat 9-318615 is measured the calcium ion concentration of this test solution.If there is sediment (as zeolite),, it is filtered from this solution by filtering with miillpore filter (0.45 μ m).

Embodiment 18-35

The various disinfecting cleaning compositions of formulation according to table 3-7.With their aqueous solution of urban water preparation, described urban water is adjusted to 3.3 ° of DH (CaO is 33mg/L) with two hydration calcium chloride with it.Measure their bacteriolyze rate according to above identical method.The result is shown in table 3-7.Be respectively applied for following purpose at the cleaning compositions shown in the table 3-7.

By the way, in the bacteriolyze activity (U/mL) shown in these tables, be with bacteriolyze activity (U/ Unit Weight) by described enzyme, the concentration of coming out by " working concentration " shown in described table conversion.At calcium ion concentration shown in these tables and bacteriolyze rate is the result that " described test solution and the solution that is used to measure the bacteriolyze rate " is measured, described solution is to prepare according to " method that is used to measure the method for calcium ion concentration and is used to measure the bacteriolyze rate ", so that they have at the working concentration shown in the following table.Following table is carried out identical processing.

Table 3: the washing agent that is used to wash the dishes

Table 4: the washing agent that is used for washing machine

Table 3: the washing agent that is used for bath tube

Table 3: the washing agent that is used for drainpipe

Table 3: the washing agent that is used for the toilet

Embodiment 36-38 and comparing embodiment 6

The cleaning compositions that is used for clothing according to formulation shown in the table 8.After washing and drying, remain in smell on the clothing according to the appraisal procedure of mentioning below-1 inspection.In addition, with urban water (3 ° of DH) with each diluted sample to the shown working concentration of table 8.Measure the bacteriolyze activity of the described aqueous solution.The result is referring to table 8.

Appraisal procedure-1

Adding 5 intensification degree are 25 ℃ urban water (3 ° of DH) in the washing machine (CW-C30A1-H is available from Mitsubishi Electric) of twin-tub type.With each sample dissolution of described cleaning compositions in water, so that set up the shown working concentration of table 8.With gross weight is that the clothing of 1kg is immersed in the described wash solution, and described clothing is directly to contact skin to have worn five football shirts of one day and used five brand-new cotton towels of one day in the kitchen.After soaking 10 minutes, washed 10 minutes with 25 liters of urban waters.After the washing, then carry out 1 minute rotary dehydration drying, rinsing is 2 times in batches, each with 30 liters of urban water rinsings 3 minutes, and Rotary drying 2 times, once for the first time in batches between rinsing and the batch rinsing for the second time, carried out 1 minute, and once after the rinsing second time, carried out 1 minute.At last, with commercial clothesdrier (DE-N5S3 is available from Hitachi, Ltd), by dry described football shirt of standard method and towel.

After the drying, in described football shirt and towel are between the constant-temperature house of 30 ℃ and 80% relative moisture, placed 5 days.At last, check their smell by experienced testing staff.According to following standard to smelly degree grade (mean value).

The grading standard

5: quite smelly

4: obviously smelly

3: some is smelly

2: little smelly

1: not smelly or smelly hardly

Embodiment 40-47 and comparing embodiment 7

According to the composition of formulation clothes washing shown in table 9 and 10.After washing and drying, remain in smell on the clothing according to the appraisal procedure of mentioning below-2 inspection.In addition, with urban water (3 ° of DH) with each diluted sample to table 9 and 10 shown working concentrations.Measure the bacteriolyze activity of the described aqueous solution.The result is referring to table 9 and 10.

Appraisal procedure-2

With automatic washing machine (NA-F70A is available from Matsushita Electric) washing gross weight is the clothing of 1kg, and described clothing is directly to contact skin to have worn five football shirts of one day and used five brand-new cotton towels of one day in the kitchen.Washing is to be approximately 25 ℃ urban water (3 ° of DH) with temperature to carry out according to standard method.Add each sample of described cleaning compositions, so that set up table 9 and 10 shown working concentrations.After Rotary drying, with described football shirt and towel in 30 ℃ and 80% relative humidity room dry 12 hours.At last, check their smell by experienced testing staff.According to following standard to smelly degree grade (mean value).

The grading standard

5: quite smelly

4: obviously smelly

3: some is smelly

2: little smelly

1: not smelly or smelly hardly

Embodiment 48-51 and comparing embodiment 8

According to the clothes washing of formulation shown in the table 11 composition.After washing and drying, remain in smell on the clothing according to the appraisal procedure of mentioning below-3 inspection.Measure their bacteriolyze activity according to same procedure above-mentioned.The result is referring to table 11.

Appraisal procedure-3

With roller washing machine (ES-E61 is available from Sharp company) washing 10 brand-new cotton towels (having used 1 day in daily life).Washing is to be approximately 40 ℃ urban water (being adjusted to 20 ° of DH with two hydration calcium chloride) with temperature to carry out according to standard wash circulation.The consumption of every kind of sample of described cleaning compositions is enough to reach the concentration of 5000ppm.After Rotary drying, with washed towel in 30 ℃ and 80% relative humidity room dry 12 hours.At last, check their smell by experienced testing staff.According to following standard to smelly degree grade (mean value).

The grading standard

5: quite smelly

4: obviously smelly

3: some is smelly

2: little smelly

1: not smelly or smelly hardly

Being described as follows of composition among the his-and-hers watches 1-11.The amount of each composition is represented (except SODIUM PERCARBONATE and the bleach activator) with the nt wt net weight form.

Lauric acid sodium sulphate: net weight 95%, available from Lion company; " Sunnol LM-1100NT " α-SF-Na: the sodium salt of alpha-sulfo fatty acid methyl ester has C _14-16Alkyl group; Available from Lion company; Net weight 70%; Solid 72-73%.

LAS-K: straight chain C _10-14Alkyl benzene sulphonate potassium (when producing relevant cleaning compositions) by what prepare available from the Lipon LH-200 (LAS-H net weight 96%) of Lion company with 48% potassium hydroxide aqueous solution neutralization.

LAS-Na: straight chain C _10-14Sodium alkyl benzene sulfonate (by with the neutralization of 48% sodium hydrate aqueous solution available from Lipon LH-200 (the LAS-H net weight 96%) preparation of Lion company).

AOS-Na: the alpha-olefin sodium sulfonate has C _14-18Alkyl group; Available from Lion company; Net weight 55% contain water suspension paste.

AES-Na: polyoxyethylene (EO3mol) sodium laureth sulfate, net weight 70% is available from Lion company.

The PTS:p-toluene fulfonate.

Cumene sulfonic acid; Available from Albright ﹠amp; Wilson company.

Soap: have C _12-18The fatty acid sodium salt of alkyl group (available from Lion company); C ₁₂: 0.9%, C ₁₈: 80.2%, unsaturated fatty acid: 80.2%, molecular weight: 289, net weight 67-68% tires: 47.0 ℃.

N-lauryl dimethyl acetate betain: available from Sanyo chemical industrial company.

Alkyl amino propyl-acetic acid betain: available from Ipposha Oil industrial group.

BRE15:C ₁₂Straight chain primary alcohol ethoxylate (having the oxirane that 15 moles (on average) added) is available from Lion company.

The addition product of AEt1:Diadol 13 (available from Mitsubishi chemical company) and 15 moles of (on average) oxirane (available from Lion chemical company).

AEt2:C _12-14The addition product of straight chain alcohol and 8 moles of (on average) oxirane (available from Lion chemical company).

AEs:C _12-18The addition product of fatty acid methyl ester and 15 moles of (on average) oxirane (available from Lion chemical company).

The addition product (available from Lion chemical company) of AEP:Diadol 13 (available from Mitsubishi chemical company) and 15 moles of (on average) oxirane and 3 moles of (on average) expoxy propane.

APG: alkyl polyglucoside (available from Cognis Japan).

LDE: lauryl diglycollic amide (available from Kawaken Fine Chemical).

Dicyandiamide: available from Nippon Carbide Industries.

CDE: coconut fatty acid diethanol amine (available from Kawaken Fine Chemical)

AX: lauryl dimethyl amine oxide (available from Lion company).

＜enzyme (protease) 〉

Spill Wei Naisi 12T, Esperase 4T, Everlase 8T, Kannase 24TK, Alcalase2T spills Wei Naisi 16L, and Deozyme: the enzyme preparation (available from Novozymes company) that is used for washing agent.

P-enzyme: produce by bacillus P, by the preservation of fermentation research institute, preserving number 8090; Be to open: method cultivation among the flat 8-165493 and purifying by being disclosed in the Japan Patent spy.

K-enzyme: produce by bacillus K, by the preservation of fermentation research institute, preserving number 8091; Be to open: method cultivation among the flat 8-165493 and purifying by being disclosed in the Japan Patent spy.

X-enzyme: produce by bacillus X, by the preservation of fermentation research institute, preserving number 8092; Be to open: method cultivation among the flat 8-165493 and purifying by being disclosed in the Japan Patent spy.

Y-enzyme: produce by bacillus Y, by the preservation of fermentation research institute, preserving number 8088; Be to open: method cultivation among the flat 8-165494 and purifying by being disclosed in the Japan Patent spy.

Thermolysin: available from Sigma company.

＜enzyme (other enzymes) 〉

The mixture of 10: 1 (weight) of amylase: Duramyl 60T (available from Novozymes company) and amylopectase (available from Sigma company).

Cellulase: cellulase 0.7T (available from Novozymes company).

Lipase: Lipex50T (available from Novozymes company).

＜chelating agent 〉

ASDA: aspartic acid sodium diacelate (available from Nippon Shokubai).

HIDS: oxyimino sodium succinate (available from Nippon Shokubai).

EDTA-Na: sodium ethylene diamine tetracetate.

Phenanthroline: 1,10-phenanthroline (available from Nippon Kayaku).

Sodium phosphate trimer: available from Nippon Chemical

Citric acid and sodium salt thereof (available from Fuso Chemical).

Zeolite: zeolite A (Silton B is available from Mizusawa Kagaku).

AA/MA: copolymer salt: the sodium salt of acrylic acid-maleic acid (Sokalan CP7 is available from BASF).

Sodium Polyacrylate: Sodium Polyacrylate (Aronbis S is available from Nihon Junyaku).

Malic acid: available from Fuso Chemical.

＜other compositions 〉

Sodium carbonate: sodium carbonate (particle form is available from Asahi Glass).

Potash: potash (available from Asahi Glass).

Sodium silicate: sodium silicate (JIS-1) (available from Nippon Chemical).

Sodium sulphate: neutral anhydrous Glauber ' s salt (available from Nippon Chemical).

Sodium chloride: sodium chloride (preparation).

Sodium sulphite: sodium sulphite acid anhydrides (available from Mitsui Chemical).

Ethanol: purity 99.5% (available from Japanese Ethanol).

Diethylene glycol one butyl ester (available from Nippon Nyukazai).

Fluorescent brightener CBS: Cinopearl CBS-X (available from Ciba-SpecialtyChemicals).

Fluorescent whitening agent AMS:Cinopearl AMS-GX (available from Ciba-SpecialtyChemicals).

SODIUM PERCARBONATE: the SODIUM PERCARBONATE of dressing (SPC-D is available from Mitsubishi GasChemical).

Bleach activator OBC: the particle of Huo Deing by the following method, average particulate diameter is 700 μ m.With 4-caprinoyl p-methoxybenzoic acid (as bleach activator), the C of polyethylene glycol (PEG) #6000 (available from Lion company) and powder type ₁₄Alpha-olefin sodium sulfonate (Lipolan PB-80 is available from Lion company) is by mixing the mixture of preparation 70/25/5 (wt) in Extrud-O-mix (Model EM-6 is available from HosokawaMicron).Then described mixture is squeezed into the bar that diameter is 0.8mm.By using Fitzmill, Model DKA-3 (available from Hosokawa Micron) pulverizes described the zeolite A (powder type) with 5wt%.

Flavor compositions

Spices among table 3-5 and the 7-11: be disclosed in the Japan Patent spy and open: the composition in the table 11-18 among the 2002-146399.

Fatty acid amide CAB: available from Lion company.

Amidopropyl acetate betain: available from Lion company.

Alkyl phenoxy phenyl sodium disulfonate: available from Nikko Chemicals.

Stearyl trimethyl ammonium chloride: available from Lion Akzo.

Two caprinoyl ammonio methacrylates: available from Lion Akzo.

Synthetic polymer: methacrylic acid copolymer: available from Ipposha Oil industrial group.

P-methyl benzenesulfonic acid: available from Kyowa Hakko Kogyo.

Triethanolamine: available from Nippon Shokubai.

Silica: the silicone graft polymer is available from Ipposha Oil industrial group.

0.1N sodium hydroxide: preparation.

NRE2: available from Lion company.

NRE5: available from Lion company.

NRE7: available from Lion company.

NRE15: available from Lion company.

Polyethylene glycol: available from NOF company.

Diethanol amine: available from Nippon Shokubai.

Triethanolamine: available from Nippon Shokubai.

From table 1 and 2 as can be seen, if the concentration of the lysozyme that the described aqueous solution contains (anti-chelating agent) makes the bacteriolyze activity be not less than 2.5U/mL, and calcium ion concentration (referring to CaO) is no more than 10ppm, and the pH value is higher than 8, but be no more than 12, the described aqueous solution just has outstanding bacteriolyze activity.It can also be seen that, if the concentration of the lysozyme that the described aqueous solution contains (anti-chelating agent) makes the bacteriolyze activity be not less than 15U/mL, and calcium ion concentration (referring to CaO) is no more than 10ppm, and the pH value is higher than 12, and the described aqueous solution just has outstanding bacteriolyze activity.

As can be seen, the cleaning compositions among the embodiment has outstanding bacteriolyze effect from table 3-7.Described effect confirms by the shown cleaning compositions of table 3, and is after said composition is used to use 6 months, smelly and feel the sticky sponge of washing the dishes.After (the being used for family of four) week that described sponge is used for wash the dishes with described cleaning compositions, stink and sticky sense disappear.If the shown aqueous solution of table 2 is sprayed at kitchen basin, refuse container and draining wire netting, and rinsing after 10 minutes can weaken sticky sense greatly.

The result of table 8-11 shows that the cleaning compositions among the embodiment can produce the effect of good inhibition stink.

Table 1

The aqueous solution is formed	Embodiment
											??1	??2	??3	??4	??5	??6	??7	??8	??9	??10
	Surfactant (ppm)
Non-ionic surface active agent (BRE15)												??250	??250	??250	??250	??250	??250	??250	??250	??250	??250
	Month silica-based sodium sulphate
Alkaline components (ppm)
											Sodium carbonate	??250	??250	??250	??250	??20	??30			??250	??250
Potash								??250
											Sodium silicate							??250
Chelating agent (ppm)
											HIDS
ASDA
											EDTA-4Na	??250	??250			??250	??250	??250	??250	??250	??250
Sodium phosphate trimer
											Zeolite
Citramalase			??130	??130
											Lysozyme (U/mL)
Savinase?12T	??3	??13.3	??20	??30
											Esperase?4T					??10.5
Everlase?8T						??8.3
											Kannase?24TK							??6.6
Alcalase?2T								??5.3
											The K-enzyme									??15
The P-enzyme										??15
											The X-enzyme
The Y-enzyme
											Calcium ion concentration (CaO ppm)	??0.01	??0.01	??12	??12	??0.01	??0.01	??0.01	??0.01	??0.01	??0.01
The pH of the aqueous solution	??10.5	??10.5	??10.5	??10.5	??8.5	??9.0	??11.0	??10.5	??10.5	??10.5
											Bacteriolyze rate (%)	??40	??60	??40	??50	??40	??50	??45	??45	??65	??65

Table 2

The aqueous solution is formed	Embodiment							Comparing embodiment
													??11	??12	??13	??14	??15	??16	??17	??1	??2	??3	??4	??5
	Surfactant (ppm)
Non-ionic surface active agent (BRE15)														??250	??250		??250	??250	??250	??250	??250			??250	??250
	Month silica-based sodium sulphate			??250
Alkaline components (ppm)
													Sodium carbonate	??250	??250	??250	??250	??250			??250	??15	??15	??250	??250
Potash						??250
													Sodium silicate							??250
Chelating agent (ppm)
													HIDS	??250
ASDA		??250
													EDTA-4Na			??250					??250				??250
Sodium phosphate trimer				??250
													Zeolite					??250
Sodium citrate						??250
													Phenanthroline							??250				??250
Lysozyme (U/mL)
													Savinase?12T			??13.3	??13.3	??13.3	??13.3	??20			??13.3
Esperase?4T
													Everlase?8T
Kannase?24TK
													Alcalase?2T
The K-enzyme
													The P-enzyme
The X-enzyme	??15
													The Y-enzyme		??15
Thermolysin												??*
													Calcium ion concentration (CaO ppm)	??0.05	??0.08	??0.01	??0.03	??6	??5	??30	??0.01	??30	??30	??30	??0.01
The pH of the aqueous solution	??10.5	??10.5	??10.5	??10.4	??10.4	??10.4	??10.5	??10.5	??8.0	??8.0	??10.5	??10.5
													Bacteriolyze rate (%)	??65	??65	??60	??60	??55	??55	??60	??3	??1	??7	??1	??1

Table 3

Prescription (wt%)	Embodiment
				????18	????19	????20
	??AES-Na	????10	????2				????15
??LAS-Na				????5
	??AOS-Na	????5					????10
??Soap					????2
	??AX	????5	????1				????5
Sulfobetaines
	Non-ionic surface active agent	????10	????1
??APG					????30
	??LDE	????10					????2
Cumene sulfonic acid
	??PTS	????5	????5				????5
Perfume composition				????A	????B	????A
	The perfume amount	????0.1	????0.1				????0.1
Chelating agent
				??HIDS	????0.5
??ASDA	????0.5
				Citric acid	????3	????0.3	????0.3
??EDTA-4Na
				Sodium hydroxide	PH regulator	PH regulator	PH regulator
Purified water	Balance	Balance	Balance
				Lysozyme (U/g)
??Savinase?12T	????300	????150
				The Y-enzyme		????150	????300
Amount to (%)	????100	????100	????100
				Working concentration (ppm)	????50000	????50000	????50000
Calcium ion concentration (CaO ppm)	????0.02	????8.9	????8.9
				The pH of the aqueous solution	????8.1	????8.1	????8.1
Lysozyme activity (U/mL)	????15	????15	????15
				Bacteriolyze rate (%)	????50	????40	????40

Table 4

Prescription (wt%)	Embodiment
						????21	????22	????23	????24	????25
	Non-ionic surface active agent (BRE15)	????5	????5	????5	????5						????20
Dicyandiamide						????20	????20	????20	????20
	Basic formulations
SODIUM PERCARBONATE							????50	????50	????50	????50
	Sodium carbonate	????10	????10	????10	????10
Sodium bicarbonate							????10	????10	????10	????10
	Perfume composition	????A	????B	????C	????D	????A
The perfume amount							????0.1	????0.1	????0.1	????0.1	????0.1
	Chelating agent
Malic acid											????20
	Sodium citrate	????5	????5	????5	????5	????5
0.1N sodium hydroxide											PH regulator
	Water	Balance	Balance	Balance	Balance	Balance
Lysozyme (U/g)
						Savinase?12T	????2000
The K-enzyme		????2000
						The P-enzyme			????2000
The X-enzyme				????2000
						The Y-enzyme					????2000
Amount to (%)	????100	????100	????100	????100	????100
						Working concentration (ppm)	????5000	????5000	????5000	????5000	????5000
Calcium ion concentration (CaO ppm)	????3.9	????3.9	????3.9	????3.9	????3.9
						The pH of the aqueous solution	????10.7	????10.7	????10.7	????10.7	????8.5
Lysozyme activity (U/mL)	????10	????10	????10	????10	????10
						Bacteriolyze rate (%)	????60	????60	????60	????60	????50

Table 5

Prescription (wt%)	Embodiment
			????26	????27
	??LAS-Na	????0.5			????0.5
??AOS-Na			????0.5	????0.5
	??AES-Na	????0.5			????0.5
??Soap			????0.5	????0.5
	Non-ionic surface active agent (BRE15)	????0.5			????0.5
??N-laryldimethylacetate?betaine			????0.5
	??Alkylamidopropyle?acetate?bataine	????0.5
??CDE			????0.5	????0.5
	??Benzalconium?chloride	????0.5
Diethanol one butyl ester			????0.5	????0.5
	Ethanol	????0.5			????0.5
Perfume composition			????A	????D
	The perfume amount	????0.1			????0.1
＜chelating agent 〉
			??EDTA-4Na	????1	????1
Sodium citrate	????1	????1
			Purified water	Balance	Balance
0.1N potassium hydroxide	PH regulator	PH regulator
			＜lysozyme〉(U/g)
??Savinase?12T	????2000
			??Y-enzyme		????2000
Amount to (%)	????100	????100
			Working concentration (ppm)	????50000	????50000
Calcium ion concentration n (caO ppm)	????0.01	????0.01
			Aqueous solution pH	????8.5	????8.5
Bacteriolyze activity (U/mL)	????10	????10
			Bacteriolyze rate (%)	????55	????55

Table 6

Prescription (wt%)	Embodiment
						????28	????29	????30	????31	????32
	Polyoxyethylene oleoyl ether (average 50 moles of EO)	????1	????1	????1	????1						????1
AOS-Na						????1	????1	????1	????1	????1
	Sulfuric acid ketone acid acid anhydride	????0.1	????0.1	????0.1	????0.1						????0.1
Sodium Benzoate						????1	????1	????1	????1	????1
	Inclined to one side aluminosilicate magnesium	????1	????1	????1	????1						????1
Blue?No.404						????0.002	????0.002	????0.002	????0.002	????0.002
	＜alkaline components 〉
Sodium orthosilicate							????75	????75	????75	????75	????75
	SODIUM PERCARBONATE	????10	????10	????10	????10	????10
＜chelating agent 〉
						Sodium citrate	????1	????1	????1	????1	????1
EDTA-4Na	????1	????1	????1	????1	????1
						Water r	Balance	Balance	Balance	Balance	Balance
＜lysozyme〉(U/g)
						Savinase?12T	????2000
The K-enzyme		????2000
						The P-enzyme			????2000
The X-enzyme				????2000
						The Y-enzyme					????2000
Amount to (%)	????100	????100	????100	????100	????100
						Working concentration (ppm)	????50000	????50000	????50000	????50000	????50000
Calcium ion concentration (CaO ppm)	????0.01	????0.01	????0.01	????0.01	????0.01
						The pH of the aqueous solution	????11.5	????11.5	????11.5	????11.5	????11.5
Bacteriolyze activity (U/mL)	????10	????10	????10	????10	????10
						Bacteriolyze rate (%)	????65	????65	????65	????65	????65

Table 7

Prescription (wt%)	Embodiment
				????33	????34	????35
	AOS-Na						????1
The fatty acid amide betain						????1
	Amido propyl acetate betain	????0.5
AES-Na				????2
	Alkyl phenoxy phenyl sodium disulfonate	????0.5
APG					????1.5
	Soap	????0.1	????0.1				????0.1
Stearyl trimethyl ammonium chloride						????0.1
	DDAC						????0.1
Ethanol				????4	????2	????6
	Synthetic polymer		????0.5				????0.1
P-methyl benzenesulfonic acid					????0.05
	Triethanol ammonium		????0.5
Silica					????10
	Perfume composition	????A	????B				????D
The perfume amount				????0.1	????0.1	????0.1
	＜chelating agent 〉
Citric acid					????1	????1	????0.3
	EDTA-4Na	????1	????1	????0.3
0.1N sodium hydroxide					PH regulator	PH regulator	PH regulator
	Purified water	Balance	Balance	Balance
＜lysozyme〉(U/g)
				Savinase?12T
The K-enzyme	????2000
				The P-enzyme		????2000
The X-enzyme			????2000
				The Y-enzyme
Amount to (%)	????100	????100	????100
				Working concentration (ppm)	????50000	????50000	????50000
Calcium ion concentration (CaO ppm)	????0.01	????0.01	????0.01
				The pH of the aqueous solution	????9.0	????9.0	????9.0
Bacteriolyze activity (U/mL)	????10	????10	????10
				Bacteriolyze rate (%)	????55	????55	????55

Table 8

Prescription (wt%)	Embodiment				Comparing embodiment
						????36	????37	????38	????39	????6
	??NRE2
??NRE5							????15	????20	????20	????20
	??NRE7	????35	????15
??BRE15									????15	????15
	??AES-Na			????15
Soap						????0.5	????0.5	????0.5	????0.5	????0.5
	Ethanol	????5	????5	????5	????5						????5
Polyethylene glycol						????5	????5	????5	????5	????5
	Perfume composition	????A	????B	????C	????D						????A
The perfume amount						????0.2	????0.2	????0.2	????0.2	????0.2
	Dyestuff	????0.0001	????0.0001	????0.0001	????0.0001						????0.0001
＜alkaline components 〉
						Diethanol amine	????1		????1	????1	????1
Triethanolamine		????1
						＜chelating agent 〉
??HIDS				????1
						??ASDA				????1
Sodium citrate	????1			????1
						The AA/MA copolymer salt	????2	????3	????4		????4
Purified water	Balance	Balance	Balance	Balance	Balance
						＜lysozyme〉(U/g)
??Savinase?16L	????4500
						The K-enzyme		????4500
The P-enzyme			????2250
						The X-enzyme			????2250
The Y-enzyme				????4500
						Amount to (%)	????100	????100	????100	????100	????100
Working concentration (ppm)	????4000	????4000	????4000	????4000	????4000
						Calcium ion concentration (CaO ppm)	????6	????2	????0.02	????3	????0.02
The pH of the aqueous solution	????8.5	????8.5	????8.5	????8.5	????8.5
						Bacteriolyze activity (U/mL)	????18	????18	????18	????18	????0
Bacteriolyze rate (%)	????55	????55	????55	????55	????2
						The football shirt peculiar smell	????2	????2	????1	????2	????5
The towel peculiar smell	????1	????2	????2	????1	????5

Table 9

Prescription (wt%)	Embodiment				Comparing embodiment
						????40	????41	????42	????43	????7
	α-SF-Na	????12	????12	????10	????10						????10
LAS-K								????8	????8	????8
	Soap	????8	????8	????8	????8						????8
AOS-Na								????1	????1	????1
	AEt1	????6		????2	????5						????5
AEt2
	AEs		????6	????2
AEP								????2
	Sodium sulphite	????1	????1		????1						????1
Fluorescent whitening agent, CBS						????0.1	????0.1	????0.1	????0.2	????0.2
	Fluorescent whitening agent, AMS	????0.1	????0.1	????0.1	????0.2						????0.2
Amylase						????0.5	????0.5	????0.5	????0.5	????0.5
	Cellulase	????0.5	????0.5	????0.5	????0.5						????0.5
Lipase						????0.5	????0.5	????0.5	????0.5	????0.5
	SODIUM PERCARBONATE			????3
Bleach activator, OBC								????1
	Perfume composition	????C	????D	????B	????A						????A
The perfume amount						????0.2	????0.1	????0.1	????0.2	????0.2
	＜alkaline components 〉
Sodium carbonate							Balance	Balance	Balance	Balance	Balance
	Potash	????8	????8	????8	????8	????8
Sodium silicate
	＜chelating agent 〉
HIDS								????1
	ASDA		????1
Zeolite							????35	????35	????35	????22	????22
	The AA/MA copolymer salt	????6		????5	????3	????3
Sodium Polyacrylate								????4
	Water	????7	????7	????7	????7	????7
＜lysozyme〉(U/g)
						Savinase?12T	????20000			????40000
Kannase?24TK		????20000
						The Y-enzyme			????15000
Amount to (%)	????100	????100	????100	????100	????100
						Working concentration (ppm)	????500	????500	????667	????500	????500
Calcium ion concentration (CaO ppm)	????9.0	????9.0	????8.5	????14.0	????14.0
						The pH of the aqueous solution	????10.5	????10.5	????10.5	????10.5	????10.5
Bacteriolyze activity (U/mL)	????10	????10	????10	????20	????0
						Bacteriolyze rate (%)	????55	????55	????55	????60	????7
The football shirt peculiar smell	????2	????2	????1	????2	????4
						The towel peculiar smell	????1	????1	????2	????1	????4

Table 10

Prescription (wt%)	Embodiment
					????44	????45	????46	????47
	α-SF-Na	????12	????12	????10					????10
LAS-K							????8	????8
	Soap	????8	????8	????8					????8
AOS-Na							????1	????1
	AEt1	????6		????2					????5
AEt2
	AEs		????6	????2
AEP							????2
	Sodium sulphite	????1	????1						????1
Fluorescent whitening agent, CBS					????0.1	????0.1	????0.1	????0.2
	Fluorescent whitening agent, AMS	????0.1	????0.1	????0.1					????0.2
Amylase					????0.5	????0.5	????0.5	????0.5
	Cellulase	????0.5	????0.5	????0.5					????0.5
Lipase					????0.5	????0.5	????0.5	????0.5
	SODIUM PERCARBONATE			????3
Bleach activator, OBC							????1
	Perfume composition	????C	????D	????B					????A
The perfume amount					????0.2	????0.1	????0.1	????0.2
	＜alkaline components 〉
Sodium carbonate						Balance	Balance	Balance	Balance
	Potash	????8	????8	????8	????8
Sodium silicate
	＜chelating agent 〉
HIDS							????1
	ASDA		????1
Zeolite						????35	????35	????35	????22
	The AA/MA copolymer salt	????6		????5	????3
Sodium Polyacrylate							????4
	Water	????7	????7	????7	????7
＜lysozyme〉(U/g)
					Deozyme	????20000	????20000	????20000	????20000
Amount to (%)	????100	????100	????100	????100
					Working concentration (ppm)	????500	????500	????667	????500
Calcium ion concentration (CaO ppm)	????9.0	????9.0	????8.5	????14.0
					The pH of the aqueous solution	????10.5	????10.5	????10.5	????10.5
Bacteriolyze activity (U/mL)	????10	????10	????20	????20
					Bacteriolyze rate (%)	????60	????60	????60	????60
The football shirt peculiar smell	????2	????2	????1	????1
					The towel peculiar smell	????1	????1	????1	????2

Table 11

Prescription (wt%)	Embodiment				Comparing embodiment
						????48	????49	????50	????51	????8
	α-SF-Na	????5	????4	????5							????5
LAS-K							????5	????2	????5	????1
	Soap	????3	????1	????2	????3						????2
AOS-Na								????1	????1	????1
	AEt1	????2		????1	????1						????1
AEt2
	AEs		????1	????1
AEP								????1
	Sodium sulphite	????1	????1		????1						????1
Fluorescent whitening agent, CBS						????0.1	????0.1	????0.1	????0.2	????0.2
	Fluorescent whitening agent, AMS	????0.1	????0.1	????0.1	????0.2						????0.2
Amylase						????0.5	????0.5	????0.5	????0.5	????0.5
	Cellulase	????0.5	????0.5	????0.5	????0.5						????0.5
Lipase						????0.5	????0.5	????0.5	????0.5	????0.5
	SODIUM PERCARBONATE	????10	????10	????10	????10						????10
Bleach activator, TAED						????5	????5	????5	????5	????5
	Perfume composition	????C	????D	????B	????A						????A
The perfume amount						????0.2	????0.1	????0.1	????0.2	????0.2
	＜alkaline components 〉
Sodium carbonate							Balance	Balance	Balance	Balance	Balance
	Potash	????1	????1	????1	????1	????1
Sodium silicate							????5	????10	????5	????5	????5
	＜chelating agent 〉
HIDS							????2	????1	????4	????5	????5
	ASDA	????2	????1	????1	????2	????2
Zeolite							????30	????30	????30	????20	????20
	The AA/MA copolymer salt	????6	????6	????7	????5	????5
Sodium Polyacrylate							????2	????4
	Water	????7	????7	????7	????7	????7
＜lysozyme〉(U/g)
						Deozyme	????2000	????2000	????26000	????26000
Amount to (%)	????100	????100	????100	????100	????100
						Working concentration (ppm)	????5000	????5000	????5000	????5000	????5000
Calcium ion concentration (CaO ppm)	????5	????8	????5	????5	????5
						The pH of the aqueous solution	????10.5	????10.5	????10.5	????10.5	????10.5
Bacteriolyze activity (U/mL)	????10	????10	????130	????130	????0
						Bacteriolyze rate (%)	????65	????65	????75	????75	????8
The towel peculiar smell	????2	????2	????1	????1	????4

Claims (10)

1. a sterilization method comprises

Handle the material that is subjected to germ contamination with a kind of aqueous solution, this solution contains the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contain the calcium ion that concentration is no more than the CaO form of 10ppm, and the pH value is higher than 8, but is no more than 12.

2. a sterilization method comprises

Handle the material that is subjected to germ contamination with a kind of aqueous solution, this solution contains the lysozyme that can tolerate chelating agent, and the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12.

3. not phosphatic disinfecting cleaning composition, under its common form or its aqueous solution form, contain the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contain the calcium ion that concentration is no more than the CaO form of 10ppm, and the pH value is higher than 8, but is no more than 12.

4. not phosphatic disinfecting cleaning composition, under its common form or its aqueous solution form, contain the lysozyme that can tolerate chelating agent, the concentration of described enzyme is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12.

5. as the disinfecting cleaning composition of claim 3 or 4, also comprise surfactant.

6. as disinfecting cleaning composition any among the claim 3-5, also comprise lipase, its enzymic activity is such: the amount of the fatty acid that discharges from triolein after the test substrate decomposes is not less than 2 times of amount (with molal quantity calculating) of the fatty acid that discharges from n-cetyl palmitate after the test substrate decomposes.

7. as disinfecting cleaning composition any among the claim 3-6, also comprise coming from the wild type amylase that carried out the bacillus of modification by sudden change, or energy cracking α-1,6 connects the amylolytic enzyme of key.

8. as disinfecting cleaning composition any among the claim 3-7, it is used to decompose and eliminate dead bacterial cell.

9. as disinfecting cleaning composition any among the claim 3-7, be used for sterilization and washing during textile in common form or its dilute aqueous solution form, avoid washed textile to give off a peculiar smell at the storage period during the indoor seasoning or after drying with it.

10. one kind with the not phosphatic method that contains the disinfecting cleaning composition washing textile of the lysozyme that is subjected to chelating agent of having the capability of doing sth, described method comprises the solution washing textile that is used to sterilize with a kind of, described solution prepares with described disinfecting cleaning composition, so that

(a) it contains the lysozyme that can tolerate chelating agent, and described enzyme concentration is enough to make its bacteriolyze activity level to be not less than 2.5U/mL, and contains the calcium ion that concentration is no more than the CaO form of 10ppm, and the pH value is higher than 8, but is no more than 12, or:

(b) it contains the lysozyme that can tolerate chelating agent, and described enzyme concentration is enough to make its bacteriolyze activity level to be not less than 15U/mL, and the pH value is higher than 8, but is no more than 12,

Thereby avoid during indoor seasoning or after drying storage period, textile gives off a peculiar smell.