JP2700465B2 - Toiletries containing lysozyme chloride - Google Patents

Description

The present invention relates to a toiletry product containing lysozyme chloride.

(B) Conventional technology and its problems Conventionally, lysozyme chloride has been widely distributed in living organisms and is an enzyme that catalyzes the hydrolysis of polysaccharides that are components of bacterial cell membranes. As specific actions, a lytic action, an anti-inflammatory action, a hemostatic action, a tissue repair action, and the like are known, and as its application field, it is widely used in fields such as pharmaceuticals and foods.

However, as for the dosage form, powder, granules and tablets occupy most of the market. This is because in the formulation of toiletries such as cream, emulsion, jelly, and liquid, the activity of lysozyme chloride is inhibited by water and surfactant,
This is because long-term stabilization of lysozyme chloride is difficult.

Therefore, in the past, a long-term stabilization method for lysozyme chloride in such creamy, milky, jelly-like, and liquid toiletry products and the development of toiletry products based on the technology have been promoted. ,
It has been reported that lysozyme solutions to which ethanol, sugars, etc. are added are stable.

However, in toiletry products containing a large amount of surfactant and water, a satisfactory long-term stabilizing effect cannot be obtained, and in some products, there is a problem in use feeling.

Furthermore, unlike pharmaceuticals and foods, the storage conditions at the time of market distribution are not constant, and are different from those of pharmaceuticals and foods.
The mere addition of saccharides or the like is not sufficient for the activity stability of lysozyme chloride and is not practical.

(C) Means for solving the problems The present inventor has found that when lysozyme chloride is blended in each toiletry product, the activity of lysozyme chloride is prolonged in the presence of moisture, a surfactant, and unstable storage conditions. As a result of continuing research and development for the purpose of stabilization, by formulating the type and content of surfactant, water content, pH, type and amount of stabilizer for each product according to the purpose,
A long-term stabilization method for lysozyme chloride in toiletry products was found.

That is, the present invention provides a) lysozyme chloride, b) at least one selected from the group consisting of a nonionic surfactant, a cationic surfactant and an amphoteric surfactant, and c).
The present invention relates to a lysozyme chloride-containing toiletry product containing at least two kinds selected from the group consisting of ethanol, saccharides, amino acids and chelating agents and having a pH of 5 ± 3.

Here, the toiletry products refer to toiletries such as bath detergent, facial cleanser, body cleaner, shampoo, rinse, pack, cosmetic liquid, lotion, cream, toothpaste, and clothing detergent.

In addition, the base of a toiletry product having a dosage form such as cream, jelly, emulsion, or liquid generally differs depending on the product. For example, the base is as follows.

That is, in the case of a bath agent, isopropyl myristate
A mixture of isopropyl lanolin fatty acid, liquid paraffin, diethanolamide laurate, dipropylene glycol, preservatives, pigments, fragrances, water, and the like can be considered.

In the case of facial cleanser and body detergent, glyceryl monostearate, polyoxyethylene monolaurate, propylene glycol, preservatives, dyes, fragrances,
A mixture of water and the like is conceivable.

Further, in the case of shampoo, it is conceivable to mix sodium alkyl ether sulfate, coconut fatty acid diethanolamide, preservative, pigment, fragrance, water and the like.

In the case of rinsing, a mixture of stearyldimethylbenzylammonium chloride, stearyl alcohol, glyceryl monostearate, sodium chloride, preservative, coloring matter, fragrance, water and the like can be considered.

In the case of a pack, a mixture of sodium carboxymethylcellulose, propylene glycol, polyvinyl alcohol, a preservative, a fragrance, water and the like can be considered.

In the case of a cosmetic liquid or a lotion, a mixture of dipropylene glycol, oleyl alcohol, polyoxyethylene lauryl ether, a preservative, a fragrance, water and the like can be considered.

In the case of cream, dipropylene glycol
It is possible to mix squalane, cetanol, ethanol, carboxyvinyl polymer, olive oil, preservative, fragrance, water and the like.

For toothpaste, use calcium carbonate, sodium carboxymethylcellulose, sodium lauryl sulfate,
A combination of saccharin, preservatives, fragrances, water, etc. is possible.

In the case of a laundry detergent, a mixture of sodium alkyl ether sulfate, polyoxyethylene alkyl ether, propylene glycol, fragrance, water and the like can be considered.

In addition, the component composition of the base is exemplary,
The components are not limited to the above-mentioned components at all, and the components can be changed or increased or decreased as needed.

Lysozyme chloride is, as described above, an enzyme that catalyzes the hydrolysis of polysaccharides, which are components of bacterial cell membranes. Specific actions include lytic action, anti-inflammatory action, hemostatic action, and tissue repair action. Etc.

And the content is 0.05 unit / g to 100 units / g, preferably 0.1 unit / g to 80 units / g. This differs depending on the use site or method of use of various products, and further, the amount used, and changes the concentration of lysozyme chloride according to the purpose.

Further, the toiletry product according to the present invention contains at least one or more of ethanol, sugars, amino acids, chelating agents, and sodium chloride at 70% or less, preferably
It can contain up to 50%. This can be changed as needed for each product according to the feeling of use of various products or the dosage form.

In addition, as a saccharide here, for example, reduced maltose
Xylit, sucrose, sorbitol, lactose, glucose, maltose, D-mannitol and the like can be used.
Further, as the amino acid, for example, glycine / alanine and the like can be used. As the chelating agent, for example, disodium edetate / citric acid / sodium polyphosphate / sodium metaphosphate / sodium gluconate can be used.

Further, any of nonionic, cationic and amphoteric surfactants can be used, but the total content is 60% or less, preferably 30% or less.

The nonionic surfactants include polyol fatty acid esters, lipophilic glyceryl monostearate, lipophilic glyceryl monooleate, glyceryl trimyristate, ethylene glycol monostearate, propylene glycol monostearate, and sorbitan fatty acid. Ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene fatty acid ester, polyoxyethylene alkyl ether, polyoxyethylene alkyl phenol ether, polyoxyethylene sorbitol fatty acid ester, sucrose fatty acid ester, fatty acid alkylolamide, polyoxyethylene castor oil Polyoxyethylene hydrogenated castor oil and the like are effective for long-term stabilization of lysozyme chloride.

As the cationic surfactant, benzalkonium chloride, benzethonium chloride, alkyltrimethylammonium chloride, distearyldimethylammonium chloride / stearyldimethylbenzylammonium chloride, and the like are effective for long-term stabilization of lysozyme chloride.

Examples of the amphoteric surfactant include alkyl diaminoethyl glycine chloride, lauryl dimethyl amino acetate betaine, coconut oil fatty acid amide propyl dimethylamino acetate betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine 2- Alkyl-N-sodium carboxymethyl-N-carboxymethyloxyethyl imidazolinium betaine and the like are effective for long-term stabilization of lysozyme chloride.

Further, the total water content in the final composition of the toiletry product is preferably from 0 to 90%, more preferably from 0 to 50%, but can be adjusted according to the purpose of the product.

 Further, the pH needs to be adjusted within 5 ± 3.

(D) Function and Effect With the configuration described above, the present invention has the following effects.

In the base, at least a nonionic surfactant and / or
Alternatively, the composition contains a cationic surfactant and / or an amphoteric surfactant, and has a pH within 5 ± 3, so that long-term stabilization of the action of the contained lysozyme chloride can be ensured. , Long-term storage of toiletry products.

By stabilizing the lysozyme chloride, skin damage due to various causes during use of the toiletry product can be suppressed, and the skin after use can be kept normal.

Contains two or more lysozyme chlorides, a nonionic surfactant and / or a cationic surfactant and / or an amphoteric surfactant selected from the group consisting of ethanol, saccharides, amino acids and chelating agents. Thereby, it can be applied to all toiletry products, and long-term stabilization of lysozyme chloride can be achieved.

(E) Example [Example 1] The rinsing agent according to the present invention having the composition shown in Table 1 and the activity stability of lysozyme chloride in Controls AB were compared. Furthermore, the feeling of use of Control B and the rinsing agent according to the present invention were compared.

FIG. 1 and FIG. 2 show the results of the activity stability test of lysozyme chloride, and Table 2 shows the results of the test of feeling during use.

In addition, the activity stability, M. Iysodeikticus ATCC 4698 shake culture cells, dried using acetone to form a powder suspension, lysozyme chloride was allowed to act on the suspension,
From the decrease in the degree of suspension, a method for determining the lytic enzyme activity was used, in which the titer of the sample with respect to the standard lysozyme standard was calculated each time to eliminate the deviation due to the substrate.

Therefore, in the case of a diluted sample, the amount of the sample containing lysozyme corresponding to the sampling amount of the standard lysozyme sample is sampled.

The standard lysozyme preparation preserves particularly well-purified lysozyme chloride.

 The titer of 1 mg is defined as 1,000.

As is evident from Table 2, the lysozyme chloride-containing rinse according to the present invention has properties superior to Control B with respect to the condition of the hair after rinsing which determines the quality of the rinsing agent.

As is clear from FIGS. 1 and 2, the activity stability of lysozyme chloride was 0% at room temperature and 40 ° C. for 100 days after control A and B. Only the rinsing agent can maintain the activity of lysozyme chloride.

[Second Example] The activity stability of lysozyme chloride in Controls A and B was compared with that of the pack according to the present invention having the composition shown in Table 3. Further, the feeling of use of Control B and the pack according to the present invention was compared.

3 and 4 show the results of an activity stability test of lysozyme chloride, and Table 4 shows the results of a feeling of use.

The activity stability test of lysozyme chloride was evaluated by the same method as in the first example.

As is evident from Table 4, the pack according to the present invention has superior properties to the control B with respect to the condition of the skin after packing, which determines the quality of the pack.

As is clear from FIGS. 3 and 4, the activity stability of lysozyme chloride was 0% at room temperature and 40 ° C. for 100 days after the control A and B, whereas in the present invention, Only such a pack agent can maintain the activity of lysozyme chloride.

[Third Example] The activity stability of lysozyme chloride in the lotion according to the present invention having the component constitutions shown in Table 5 and in Controls A and B was compared. Further, the feeling of use of Control B and the lotion according to the present invention was compared.

FIGS. 5 and 6 show the results of an activity stability test of lysozyme chloride, and Table 6 shows the results of a feeling of use.

The activity stability test of lysozyme chloride was evaluated by the same method as in the first example.

As is clear from Table 6, the lotion according to the present invention has excellent properties in terms of overall ease of use and feeling of use which are considered to be important items for evaluating the lotion.

Regarding the activity stability of lysozyme chloride,
As is clear from FIG. 6 and FIG.
In contrast, both room temperature and 40 ° C. are 0% after 100 days, whereas only the lotion according to the present invention can maintain the activity of lysozyme chloride.

[Fourth Example] The activity stability of lysozyme chloride in the nutritional cream according to the present invention having the composition shown in Table 7 and the control AB was compared. Furthermore, the feeling of use between the control B and the cream according to the present invention was compared.

7 and 8 show the results of an activity stability test of lysozyme chloride, and Table 8 shows the results of a test of feeling during use.

The activity stability test of lysozyme chloride was evaluated by the same method as in the first example.

As is clear from Table 8, the nutritional cream according to the present invention has excellent properties in terms of overall ease of use and feeling of use, which is considered to be the most important item for evaluation of the cream.

As is clear from FIGS. 7 and 8, the activity stability of lysozyme chloride was 0% after 100 days at room temperature and 40 ° C. in Controls A and B. Only the cream can maintain the activity of lysozyme chloride.

[Fifth Example] The activity stability of lysozyme chloride in the dentifrices according to the present invention having the component constitutions shown in Table 9 and in Controls A and B was compared. Furthermore, the feeling of use of Control B and the dentifrice according to the present invention were compared.

9 and 10 show the results of an activity stability test of lysozyme chloride, and Table 10 shows the results of a test of feeling during use.

The activity stability test of lysozyme chloride was evaluated by the same method as in the first example.

As is clear from Table 10, the dentifrice according to the present invention has superior properties to the control B in the mouth and the cleanliness of the teeth, which determine the quality of the dentifrice.

As is clear from FIGS. 9 and 10, the activity stability of lysozyme chloride was 0% at room temperature and 40 ° C. for 100 days after the control A and B according to the present invention. Only a dentifrice can maintain the activity of lysozyme chloride.

In addition, although similar results can be obtained in other toiletry products, preferred components of those toiletry products will be described below by way of example.

Bath agent (%) Lysozyme chloride 3.0 Glycerin 40.2 Polyoxyethylene oleyl ether 4.6 Sucrose fatty acid ester 3.6 Ethanol 13.2 Polyoxyethylene castor oil 3.2 Sorbit solution 15.5 Sodium chloride 3.1 Benzalkonium chloride 2.8 Blue No. 1 Trace amount of water Perfume 3.0 Total 100.0 (%) Face wash (%) Lysozyme chloride 0.8 Coconut oil fatty acid amidopropyl dimethylamino acetate betaine 12.4 Lauryl dimethylamino acetate betaine 20.7 Glycerin 18.2 Polyoxyethylene fatty acid ester 4.3 Polyoxyethylene hydrogenated castor oil 9.8 Sorbit liquid 25.4 Glycine 4.8 citrate Acid 2.1 Water Remaining fragrance 0.4 Total 100.0 (%) Body wash (%) Lysozyme chloride 0.8 Lauryl dimethylaminoacetic acid betaine 34.6 Glycerin 12.3 Sorbitan fatty acid ester 2.4 Polyoxyethylene sorby Fatty acid ester 6.5 Polyoxyethylene alkylphenol ether 14.3 Ethanol 10.0 Ethylene glycol monostearate 3.2 Glycine 3.4 Stearyl dimethylbenzylammonium chloride 2.1 Water Remaining Yellow No. 4 Trace amount of fragrance 0.4 Total 100.0 (%) Shampoo (%) Lysozyme chloride 0.8 2- Alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine 43.6 Ethylene glycol monostearate 4.4 Glycerin 7.7 Lauric acid diethanolamide polyoxyethylene sorbitol fatty acid ester 2.6 Sorbit solution 18.4 Alkyl diaminoethyl glycine chloride 0.8 Citric acid 3.3 Water Remaining Yellow No.4 Trace amount Perfume 0.4 Total 100.0 (%) Clothes detergent (%) Lysozyme chloride 2.1 Distearyl dimethyl ammonium chloride 23.4 Benzethonium chloride 14.3 Benzalkonium chloride 1.4 Polyoxyethylene oleyl ether 20.2 Glycine 2.3 Citric acid 1.6 Sodium chloride 4.6 Water Remaining fragrance 0.3 Total 100.0 (%) Rinse (%) Lysozyme chloride 0.5 Stearyl alcohol 3.0 Lipophilic glyceryl monostearate 3.0 Cetyl chloride Trimethylammonium 3.0 Propylene glycol 2.0 Polyoxyethylene cetyl ether 4.8 Lauryl dimethylaminoacetate betaine 1.0 Water Remaining colorant Trace amount Flavor 0.4 Total 100.0 (%) Pack (%) Lysozyme chloride 0.5 Glycerin 2.0 Hydroxyethylcellulose 3.0 Squalane 3.0 Olive oil 2.0 Polyoxy Ethylene oleyl ether 1.0 Alkyl diaminoethyl glycine chloride 0.5 Water Remaining fragrance 0.4 Total 100.0 (%) Lotion (%) Lysozyme chloride 0.5 Propylene glycol 2.0 Polyoxyethylene Rencetyl ether 0.5 l-menthol 0.1 paraben 0.2 water residual amount perfume 0.1 total 100.0 (%) cream (%) lysozyme chloride 0.5 1,3-butylene glycol 10.0 stearyl alcohol 2.0 polyoxyethylene sorbitan monolaurate 1.8 cetyl octanoate 1.0 paraben 0.3 Water Remaining amount Perfume 0.2 Lauryl dimethylamino acetate betaine 0.3 Total 100.0 (%) Dentifrice (%) Lysozyme chloride 0.5 Calcium hydrogen phosphate 20.1 Silicic anhydride 0.9 1,3-butylene glycol 11.4 Lauryl dimethylamino acetate betaine 10.0 Lauryl dimethylamine oxide 3.0 Polyoxyethylene hydrogenated castor oil 0.5 Water Remaining fragrance 0.4 Total 100.0 (%) Oral freshener (%) Lysozyme chloride 0.5 Concentrated glycerin 4.0 Benzalkonium chloride 0.5 Polyoxyethylene sorbitan monolaurate 0.5 Water Remaining fragrance 0.3 Total 100.0 (%) Emulsion (%) Lysozyme chloride 0.5 Hydroxyethyl cellulose 0.2 Propylene glycol 2.0 Polyethylene glycol monostearate 1.0 Lipophilic glyceryl monostearate 2.0 Olive oil 5.0 Oleic acid 2.0 Water Remaining fragrance 0.3 Total 100.0 (%) Body lotion (%) Lysozyme chloride 0.5 Carboxyvinyl polymer 0.2 Hydroxyethyl cellulose 0.2 Polyoxyethylene hydrogenated castor oil 2.0 Lipophilic glyceryl monostearate 1.0 Cetyl octanoate 3.0 Cetanol 0.5 Propylene glycol 2.0 Water Remaining fragrance 0.3 Total 100.0 (%) Hair lotion ( %) Lysozyme chloride 0.5 1,3-butylene glycol 2.0 Mint oil 0.1 Polyoxyethylene sorbitan monolaurate 1.0 Benzethonium chloride 0.5 Water Remaining colorant Trace amount Perfume 0.3 Total 100.0 (%) Surge Cream (%) Lysozyme Chloride 0.5 liquid paraffin 8.0 lanolin 2.0 polyoxyethylene castor oil 3.0 coconut oil fatty acid amide propyl dimethylamino acetic acid betaine 0.2 Glycerin 20.0 Water Balance Perfume 0.1 Total 100.0 (%)

[Brief description of the drawings]

FIGS. 1 and 2 are graphs showing the activity stability of lysozyme chloride in the toiletry product according to the first embodiment of the present invention at room temperature and at 40 ° C. in comparison with Controls A and B. FIGS. FIG. 4 is a graph showing the activity stability of lysozyme chloride in the toiletry product according to the second embodiment of the present invention at room temperature and 40 ° C. in comparison with Controls A and B, and FIGS. 7 and 8 are graphs showing the activity stability of lysozyme chloride in the toiletry product according to the third embodiment of the present invention at 40 ° C. and at 40 ° C., and FIGS. FIGS. 9 and 10 are graphs showing the activity stability of lysozyme chloride in the toiletry product according to the fourth embodiment of the present invention in comparison with the controls A and B. FIGS. 9 and 10 show the fifth embodiment of the present invention at room temperature and 40 ° C. Example The activity stability of lysozyme chloride of toiletry products compared to control A · B is a graph showing.

Claims (1)

(57) [Claims] 1. a) lysozyme chloride; and b) at least one selected from the group consisting of nonionic surfactants, cationic surfactants and amphoteric surfactants.
A lysozyme chloride-containing toiletry product comprising: a seed; and c) two or more kinds selected from the group consisting of ethanol, saccharides, amino acids, and a chelating agent, and having a pH of 5 ± 3.