CN1641039A - Method for extracting effective component of bamboo leaf and petiole - Google Patents
Method for extracting effective component of bamboo leaf and petiole Download PDFInfo
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Abstract
The present invention relates to the extraction of effective components in plant material, and is especially the extraction process of effective components in bamboo leaf and bamboo leaf stem. Bamboo leaf and bamboo leaf stem after pre-treatment are extracted with water in 8-70 times and filtered to obtain filtrate and filter cake, and the filtrate and the filter cake are further extracted separately to obtain the effective components. The present invention features that during water extraction, cellulase as enzymolysing agent is added to reduce water consumption and power consumption greatly. The present invention has also the advantages of high extraction efficiency, high product yield, high product purity and no environmental pollution.
Description
Technical field
The present invention relates to a kind of extracting method of effective ingredients in plant, extraction of effective components in especially a kind of leaf of bamboo, the bamboo leafstalk.
Background technology
Bamboo is Gramineae (Poaceae) Bambusoideae (Bambusoideae) perennial evergreen plant, and the whole world has 60 to belong to kind more than 1200 approximately, mainly is distributed in inferior state, and China have " bamboo kingdom ' title, kind, area and bamboo wood output all account for the world about 1/3.Have seven approximately in the economic bamboo kind of China cultivation, 80 kind, wherein mao bamboon is the good bamboo kind of China, economic worth is the highest.China's bamboo grove area has reached more than 440 ten thousand hectares at present, is used widely in industries such as building, light industry, food.
According to China's document record, light, little puckery, cold, the sweet-bitter flavor of the leaf of bamboo has clearing away heat and promoting diuresis, makes eye bright and detoxify and the hematostatic function.Considerable research has been done to the Ancient Times in China Chinese herbology by Japan, shows that the leaf of bamboo has calmness, analgesic, antibechic and hemostasis effectiveness.The relevant research data of Japan shows that Folium Bambosae extract is evident in efficacy to esophagus, bladder, kidney, lung, liver and uterus carcinoma; In addition, Folium Bambosae extract also works to stomatitis.There are some researches show that the leaf of bamboo has good corrosion prevention performance power, can add in the food as natural food preservative, sanitas, its good stability, toxicity is little, can be widely used in foodstuffs industry.In China, in the relevant comprehensive utilization of bamboo, the development and use of the leaf of bamboo, bamboo leafstalk relatively lag behind, and still are in the starting stage, and most leaf-falls become fertilizer after rotting.The leaf of bamboo, bamboo leafstalk research in recent years trends towards its nutrition and health care function gradually.
Flavonoid compound is as good living radical scavenging agent, has multiple medical treatment and health-care effects such as the immunity of adjusting and internal secretion, anticancer, anti-inflammatory, bringing into play important effect as the bioactive functions factor in emerging functional food.Some results of study show, contain abundant Flavonoid substances in the leaf of bamboo, and have shown the ability of very strong removing active oxygen radical and to the restraining effect of nitrosation reaction.Active polysaccharide is the protective foods factor of generally acknowledging, has multiple biological actions such as anti-infective, hypoglycemic, reducing blood-fat, and leaf of bamboo STUDY ON POLYSACHAROSE causes people's great attention.At present, domestic research to the leaf of bamboo, bamboo leafstalk health factor is rather active, and the leaf of bamboo, bamboo leafstalk health-care products have begun to show up prominently, and the leaf of bamboo, bamboo leafstalk may become the important source of natural antioxidants and anti-ageing healthcare functional factor fully.
Extraction of effective components in the existing leaf of bamboo bamboo leafstalk, seldom adopt zymolysis technique, for guaranteeing higher extraction yield, need add a large amount of water could propose effective constituents such as polysaccharide, flavones, protein, and needing to consume a large amount of energy could remove a large amount of water.
Summary of the invention
The object of the present invention is to provide extraction of effective components in a kind of leaf of bamboo, the bamboo leafstalk, this extracting method can obtain effective constituents such as the polysaccharide, flavones of the leaf of bamboo, bamboo leafstalk.This method extraction efficiency height, water consumption is little, can save energy.
Technical scheme of the present invention is:
Extraction of effective components in a kind of leaf of bamboo bamboo leafstalk, leaf of bamboo bamboo handle is through the pre-treatment of routine, and Jia Shuishui carries, and filters, and obtains filtrate a and filter residue b; Filtrate a and filter residue b further extract each effective constituent, and the solid-to-liquid ratio between pretreated leaf of bamboo bamboo leafstalk and the amount of water is: 1: 8-70; It is that the cellulase of 0.01-3% carries out enzymolysis that the process of carrying water adopts addition, and fiber enzymatic hydrolysis condition: PH is 3.0-6.0, temperature 30-60 degree centigrade, and time 0.5-3 hour.
The technical scheme of more excellent invention has following:
Adopt cellulase, can make the fully stripping of flavonoid compound in the leaf of bamboo.Carry with conventional water and to compare, can reduce the water consumption that water is carried the dissolving polysaccharide, and save and concentrate the required energy, also improve the flavones yield simultaneously.
Extraction of effective components in the leaf of bamboo bamboo leafstalk, water are put forward process also increases the proteolysis step, the proteolysis condition: the proteolytic enzyme amount is 0.1-2.5%, and PH is 2-5.5, temperature 25-50 degree centigrade, and time 0.3-2 hour.
Extraction of effective components in the leaf of bamboo bamboo leafstalk, filtrate a is step after filtration, be specially first ultrafiltration, it is the ultra-filtration membrane of 8000-12000 that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, it is the nanofiltration membrane of 400-8000 that molecular weight cut-off is adopted in nanofiltration, and the liquid a2 that dams can further reclaim useful matter, see through liquid through reverse osmosis concentration through what nanofiltration obtained, reverse osmosis pressure is 0.2-6mpa, and the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.
Extraction of effective components in the leaf of bamboo bamboo leafstalk, pre-treatment also comprise pulverization process, and pulverization process is pulverized leaf of bamboo bamboo leafstalk and is 4-100 purpose fine powder.
Extraction of effective components in the leaf of bamboo bamboo leafstalk, filter residue b by solid-to-liquid ratio is: 1: the consumption of 2-20 adds the ethanol that concentration is 50%-95%, under temperature 25-85 degrees celsius alcohol extracting 0.3-2 hour, refilter, the filtrate d that obtains, elder generation's ultrafiltration, it is the ultra-filtration membrane of 4000-7000 that molecular weight cut-off is adopted in ultrafiltration, the liquid b1 that dams can further reclaim useful matter, ultrafiltration obtains sees through liquid, through nanofiltration, it is the nanofiltration membrane of 400-1100 that molecular weight cut-off is adopted in nanofiltration, the further refining chromocor compound that obtains of the liquid b2 that dams, see through liquid through reverse osmosis concentration through what nanofiltration obtained, reverse osmosis pressure is 0.2-6mpa, and the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.
Extraction of effective components in the leaf of bamboo bamboo leafstalk, it is the nanofiltration membrane of 400-1100 that molecular weight cut-off is adopted in nanofiltration, the liquid a2 that dams contains chromocor compound.
Gained effective constituent of the present invention adopts following method validation effect:
The alcohol extraction process research of mao bamboon leaf flavonoid compound:
Polysaccharide extract and alcohol precipitation after, must contain the Crude polysaccharides of more impurity in 60 ℃ of following vacuum-dryings, it is dissolved in certain water.Dropwise adding Pei Lin reagent to nothing in supernatant liquor precipitates till the generation.Centrifugal collecting precipitation, it is colourless to be washed with water to water.Soaked 1 minute with 0 ℃ of dehydrated alcohol low temperature that contains 5% hydrochloric acid then, centrifugal collecting precipitation does not detect and (passes through AgNO to there being Cl-with absolute ethanol washing
3Method detects), use washing with acetone at last.The gained throw out is used water dissolution again, and flowing water dialysis 24 hours concentrates, and the dehydrated alcohol precipitation is successively with acetone, ether washing, last vacuum-drying.
With DEAE-cellulose column (3 * 50cm) on each the polysaccharide sample that makes.The DEAE-cellulose column is earlier with the phosphate buffered saline buffer balance of pH6.0, and sample is dissolved in upper prop after this damping fluid.Elution program is as follows: the phosphate buffered saline buffer wash-out of at first using 400mLpH6.0; Carrying out gradient elution again, is the phosphate buffered saline buffer of 400mL pH6.0 in the mixing pit of gradient mixer, contains the phosphate buffered saline buffer of 1M NaCl in the storage pool for equal-volume; Carry out wash-out with 1M NaOH solution at last.Elution flow rate 2mL/min, every 10mL (5min) collect a pipe.Pipe is measured polysaccharide content wherein.
(A) is ordinate zou with absorbancy, obtains the elution curve of each polysaccharide.
With Al (NO)
3-NaNO
2Colour developing colorimetry determination of total flavonoids:
The drafting of typical curve: accurately take by weighing 11.82mg and dried rutin to constant weight, place the 100ml volumetric flask, it is an amount of to add distilled water, places the water-bath heating for dissolving, places cooling, be diluted to scale then, shake up, accurately take by weighing 0.0,0.5,1.0,2.0,3.0,4.0,5.0ml rutin solution places 10ml tool plug scale test tube respectively, respectively adds 30% ethanol, make into 5.0ml, add 5%NaNO earlier
2Solution 0.3ml shakes up the back and placed 6 minutes, adds 4%NaOH solution 4.0ml, adds 30% ethanolic soln again to 10.0ml, shakes up, and places 15～20 minutes, measures optical density(OD) at wavelength 510nm place, and wherein the first pipe work is blank, draws typical curve.
Embodiment
Embodiment 1
Select 1500 kilograms of leaf of bamboo bamboo leafstalks for use, leaf of bamboo bamboo leafstalk cleans in impurity elimination, debranching, stalk, the air-dry stamp mill and fully smashs leaf, handle to pieces through the pre-treatment of routine, crosses 10 mesh sieves, adds water water after pre-treatment finishes and carries, and filters, and obtains filtrate a and filter residue b; Filtrate a and filter residue b further extract each effective constituent, it is characterized in that: the solid-to-liquid ratio between pretreated leaf of bamboo bamboo leafstalk and the amount of water is: 1: 25; It is that 0.5% cellulase carries out enzymolysis that the process of carrying water adopts addition, and fiber enzymatic hydrolysis condition: PH is 4.7,40 degrees centigrade of temperature, 2 hours time.
Filtrate a is step after filtration, is specially first ultrafiltration, and ultrafiltration employing molecular weight cut-off is 10000 ultra-filtration membrane, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1100 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 1mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.
Filter residue b by solid-to-liquid ratio is: it is 55% ethanol that 1: 8 consumption adds concentration, alcohol extracting is 1 hour under temperature 45 degrees celsius, refilter, the filtrate d that obtains, elder generation's ultrafiltration, ultrafiltration employing molecular weight cut-off is 5000 ultra-filtration membrane, the liquid b1 that dams can further reclaim useful matter, and ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1100 nanofiltration membrane, the further refining chromocor compound that obtains of the liquid b2 that dams sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 0.8mpa, the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.
After testing, the extraction rate reached 4.2% of polysaccharide.The extraction rate reached 5.8% of flavones.Isolating polysaccharide, flavones sample detect through Jiangxi Province's Food Hygiene Surveillance check, and the quality of product and sanitary index meet national related food quality hygienic standard.
Embodiment 2
Water is put forward process also increases the proteolysis step, the proteolysis condition: the proteolytic enzyme amount is 2%, and PH is 4.5,45 degrees centigrade of temperature, 1 hour time.All the other are with embodiment 1.
After testing, the extraction rate reached 5.3% of polysaccharide.The extraction rate reached 6.2% of flavones.Increase proteolysis step flavonoid yield, the extraction yield of polysaccharide has raising.
Embodiment 3
Pre-treatment is not added any enzyme after finishing, and directly leaf of bamboo bamboo leafstalk is added water water and carries.60 ℃ of the extraction temperatures that water is carried, pretreated leaf of bamboo bamboo leafstalk and add lixiviate solid-to-liquid ratio 1: 100 between the water water yield, lixiviate is after 5.5 hours, and pH value is controlled at 6.5.Filtrate a after filtration, heating concentrates 10 hours most water is removed, and enters other steps again, all the other are with embodiment 1.
For obtaining 3.481% flavonoid yield, just must could dissolve the enough flavones of leaching with a large amount of water, the lixiviate solid-to-liquid ratio was up to 1: 100.And, needing long heating evaporation in order to remove a large amount of water, energy expenditure is big.
Pre-treatment is not added any enzyme after finishing, and directly leaf of bamboo bamboo leafstalk is added water water and carries.60 ℃ of the extraction temperatures that water is carried, pretreated leaf of bamboo bamboo leafstalk and add lixiviate solid-to-liquid ratio 1: 25 between the water water yield, lixiviate is after 12 hours, and pH value is controlled at 6.5.Filtrate a after filtration, heating concentrates 10 hours most water is removed, and enters other steps again, all the other are with embodiment 1.Flavones yield 0.664%.
Embodiment 4
Filtrate a is step after filtration, is specially first ultrafiltration, sees through liquid through reverse osmosis, enters enrichment step again, and wherein ultrafiltration employing molecular weight cut-off is 10000 ultra-filtration membrane.All the other are with embodiment 1.
Adopt ultra-filtration membrane the polysaccharide of macromolecule can be separated preferably with the little flavonoid of relative molecular weight, ultra-filtration membrane and reverse osmosis logotype can be simplified concentrated program, and can reduce the lixiviate solid-to-liquid ratio, for example only need just can guarantee 6.2% flavonoid yield at 1: 25, and can concentrate at normal temperatures, do not need heating, this is with a large amount of save energy.
Embodiment 5
The embodiment of one group of solid-to-liquid ratio aspect:
(1) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 8.
(2) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 16.
(3) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 24.
(4) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 35.
(5) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 50.
(6) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 60.
(7) solid-to-liquid ratio is: all the other were with embodiment 1 in 1: 70.
Embodiment 6
The embodiment of one group of cellulase hydrolysis aspect:
(1) to carry addition in the process be 0.01% cellulase to water, and PH is 3.0,30 degrees centigrade of temperature, and 0.5 hour time, all the other are with embodiment 1.
(2) to carry addition in the process be 0.1% cellulase to water, and PH is 3.0,33 degrees centigrade of temperature, and 2 hours time, all the other are with embodiment 1.
(3) to carry addition in the process be 0.4% cellulase to water, and PH is 3.2,40 degrees centigrade of temperature, and 1 hour time, all the other are with embodiment 1.
(4) to carry addition in the process be 0.8% cellulase to water, and PH is 3.5,45 degrees centigrade of temperature, and all the other are with embodiment 1.
(5) to carry addition in the process be 1.0% cellulase to water, and PH is 3.9,55 degrees centigrade of temperature, and all the other are with embodiment 1.
(6) to carry addition in the process be 1.3% cellulase to water, and PH is 4.4,58 degrees centigrade of temperature, and all the other are with embodiment 1.
(7) to carry addition in the process be 0.4% cellulase to water, and PH is 5.2,60 degrees centigrade of temperature, and all the other are with embodiment 1.
(8) to carry addition in the process be 2.5% cellulase to water, and PH is 6.0,31 degrees centigrade of temperature, and all the other are with embodiment 1.
(9) to carry addition in the process be 3.0% cellulase to water, and PH is 6.6,30 degrees centigrade of temperature, and all the other are with embodiment 1.
Embodiment 7
The embodiment of one histone enzymolysis aspect:
(1) the proteolytic enzyme amount is 2%, and PH is 3.5,28 degrees centigrade of temperature, and 2 hours time, all the other are together
Embodiment 2.
(2) the proteolytic enzyme amount is 0.1%, and PH is 2.5,25 degrees centigrade of temperature, and 0.5 hour time, all the other are with embodiment 2.
(3) the proteolytic enzyme amount is 0.2%, and PH is 3.5,30 degrees centigrade of temperature, and 0.8 hour time, all the other are with embodiment 2.
(4) the proteolytic enzyme amount is 0.3%, and PH is 4.5,35 degrees centigrade of temperature, and 1.0 hours time, all the other are with embodiment 2.
(5) the proteolytic enzyme amount is 0.5%, and PH is 5.5,40 degrees centigrade of temperature, and 1.5 hours time, all the other are with embodiment 2.
(6) the proteolytic enzyme amount is 0.8%, and PH is 2.5,45 degrees centigrade of temperature, and 2.0 hours time, all the other are with embodiment 2.
(7) the proteolytic enzyme amount is 1.2%, and PH is 2.5,50 degrees centigrade of temperature, and 2.0 hours time, all the other are with embodiment 2.
Embodiment 8
The embodiment of one group of ultrafiltration aspect:
(1) filter residue b by solid-to-liquid ratio is: it is 85% ethanol that 1: 5 consumption adds concentration, alcohol extracting is 1 hour under temperature 60 degrees celsius, refilter, the filtrate d that obtains, elder generation's ultrafiltration, ultrafiltration employing molecular weight cut-off is 6000 ultra-filtration membrane, the liquid b1 that dams can further reclaim useful matter, and ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1050 nanofiltration membrane, the further refining chromocor compound that obtains of the liquid b2 that dams sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 0.2-6mpa, the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.All the other are with embodiment 1.
(2) filter residue b by solid-to-liquid ratio is: it is 85% ethanol that 1: 4 consumption adds concentration, alcohol extracting is 1 hour under temperature 25 degrees celsius, refilter, the filtrate d that obtains, elder generation's ultrafiltration, ultrafiltration employing molecular weight cut-off is 4000 ultra-filtration membrane, the liquid b1 that dams can further reclaim useful matter, and ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1000 nanofiltration membrane, the further refining chromocor compound that obtains of the liquid b2 that dams sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 0.2mpa, the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.All the other are with embodiment 1.
(3) filter residue b by solid-to-liquid ratio is: it is 85% ethanol that 1: 20 consumption adds concentration, alcohol extracting is 2 hours under temperature 85 degrees celsius, refilter, the filtrate d that obtains, elder generation's ultrafiltration, ultrafiltration employing molecular weight cut-off is 7000 ultra-filtration membrane, the liquid b1 that dams can further reclaim useful matter, and ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 900 nanofiltration membrane, the further refining chromocor compound that obtains of the liquid b2 that dams sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 6mpa, the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.All the other are with embodiment 1.
(4) filter residue b by solid-to-liquid ratio is: it is 85% ethanol that 1: 15 consumption adds concentration, alcohol extracting is 1 hour under temperature 50 degrees celsius, refilter, the filtrate d that obtains, elder generation's ultrafiltration, ultrafiltration employing molecular weight cut-off is 5000 ultra-filtration membrane, the liquid b1 that dams can further reclaim useful matter, and ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1050 nanofiltration membrane, the further refining chromocor compound that obtains of the liquid b2 that dams sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 0.2-6mpa, the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.All the other are with embodiment 1.
Embodiment 9
The embodiment of one group of ultrafiltration aspect:
(1) filtrate a step after filtration is specially first ultrafiltration, and it is 8000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 1100 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 0.2mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(2) filtrate a step after filtration is specially first ultrafiltration, and it is 9000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 400 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 6mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(3) filtrate a step after filtration is specially first ultrafiltration, and it is 12000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 8000 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(4) filtrate a step after filtration is specially first ultrafiltration, and it is 10500 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 7000 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 1mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(5) filtrate a step after filtration is specially first ultrafiltration, and it is 10000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 4000 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(6) filtrate a step after filtration is specially first ultrafiltration, and it is 12000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 3000 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(7) filtrate a step after filtration is specially first ultrafiltration, and it is 10000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 2000 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 4mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(8) filtrate a step after filtration is specially first ultrafiltration, and it is 12000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 500 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(9) filtrate a step after filtration is specially first ultrafiltration, and it is 12000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 700 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
(10) filtrate a step after filtration is specially first ultrafiltration, and it is 12000 ultra-filtration membrane that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, nanofiltration employing molecular weight cut-off is 800 nanofiltration membrane, the liquid a2 that dams can further reclaim useful matter, sees through liquid through reverse osmosis concentration through what nanofiltration obtained, and reverse osmosis pressure is 3mpa, the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.All the other are with embodiment 1.
Embodiment 10
It is 2 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Embodiment 11
It is 10 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Embodiment 12
It is 20 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Embodiment 13
It is 40 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Embodiment 14
It is 60 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Embodiment 15
It is 100 purpose fine powders that pulverization process is pulverized leaf of bamboo bamboo leafstalk.All the other are with embodiment 1.
Claims (6)
1, extraction of effective components in a kind of leaf of bamboo bamboo leafstalk, leaf of bamboo bamboo handle is through the pre-treatment of routine, and Jia Shuishui carries, and filters, and obtains filtrate a and filter residue b; Filtrate a and filter residue b further extract each effective constituent, it is characterized in that: the solid-to-liquid ratio between pretreated leaf of bamboo bamboo leafstalk and the amount of water is: 1: 8-70; It is that the cellulase of 0.01-3% carries out enzymolysis that the process of carrying water adopts addition, and fiber enzymatic hydrolysis condition: PH is 3.0-6.0, temperature 30-60 degree centigrade, and time 0.5-3 hour.
2, extraction of effective components in the leaf of bamboo bamboo leafstalk according to claim 1, it is characterized in that: water is put forward process also increases the proteolysis step, the proteolysis condition: the proteolytic enzyme amount is 0.1-2.5%, and PH is 2-5.5, temperature 25-50 degree centigrade, time 0.3-2 hour.
3, extraction of effective components in the leaf of bamboo bamboo leafstalk according to claim 1, it is characterized in that: filtrate a is step after filtration, be specially first ultrafiltration, it is the ultra-filtration membrane of 8000-12000 that molecular weight cut-off is adopted in ultrafiltration, dam liquid a1 vacuum or spraying drying, obtain polysaccharide, ultrafiltration obtains sees through liquid, through nanofiltration, it is the nanofiltration membrane of 400-8000 that molecular weight cut-off is adopted in nanofiltration, and the liquid a2 that dams can further reclaim useful matter, see through liquid through reverse osmosis concentration through what nanofiltration obtained, reverse osmosis pressure is 0.2-6mpa, and the liquid a3 that dams that obtains contains amino acid, and the liquid water that sees through of reverse osmosis can recycling use.
4, extraction of effective components in the leaf of bamboo bamboo leafstalk according to claim 1, it is characterized in that: pre-treatment also comprises pulverization process, pulverization process is pulverized leaf of bamboo bamboo leafstalk and is 2-100 purpose fine powder.
5, extraction of effective components in the leaf of bamboo bamboo leafstalk according to claim 1, it is characterized in that: filter residue b by solid-to-liquid ratio is: 1: the consumption of 2-20 adds the ethanol that concentration is 50%-95%, under temperature 25-85 degrees celsius alcohol extracting 0.3-2 hour, refilter, the filtrate d that obtains, elder generation's ultrafiltration, it is the ultra-filtration membrane of 4000-7000 that molecular weight cut-off is adopted in ultrafiltration, the liquid b1 that dams can further reclaim useful matter, ultrafiltration obtains sees through liquid, through nanofiltration, it is the nanofiltration membrane of 400-1100 that molecular weight cut-off is adopted in nanofiltration, the further refining chromocor compound that obtains of the liquid b2 that dams, see through liquid through reverse osmosis concentration through what nanofiltration obtained, reverse osmosis pressure is 0.2-6mpa, and the liquid b3 that dams that obtains can further reclaim useful matter, reverse osmosis see through liquid contain a large amount of ethanol can recycling use.
6, extraction of effective components in the leaf of bamboo bamboo leafstalk according to claim 3 is characterized in that: it is the nanofiltration membrane of 400-1100 that molecular weight cut-off is adopted in nanofiltration, and the liquid a2 that dams contains chromocor compound.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102532333A (en) * | 2011-09-30 | 2012-07-04 | 福建惠泽生物科技有限公司 | Method for comprehensively extracting active substances from asparagus scraps based on multi-stage membrane separation |
| CN104013065A (en) * | 2014-06-27 | 2014-09-03 | 广西健美乐食品有限公司 | Preparation method for red bamboo liquid |
| CN104041905A (en) * | 2014-06-27 | 2014-09-17 | 广西健美乐食品有限公司 | Method for preparing bambusa chungii liquid |
| CN108403978A (en) * | 2018-05-29 | 2018-08-17 | 浙江竹孝宝生物科技有限公司 | The preparation method of bamboo extractive |
| CN114052152A (en) * | 2021-09-28 | 2022-02-18 | 海南黎草纪新生物科技有限公司 | Extraction method of plant micromolecular water |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1066055C (en) * | 1998-03-17 | 2001-05-23 | 浙江农业大学 | Production method for extracting flavonoid compound extract or powder from bamboo leaf |
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- 2004-01-08 CN CNB2004100158083A patent/CN100449002C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532333A (en) * | 2011-09-30 | 2012-07-04 | 福建惠泽生物科技有限公司 | Method for comprehensively extracting active substances from asparagus scraps based on multi-stage membrane separation |
| CN104013065A (en) * | 2014-06-27 | 2014-09-03 | 广西健美乐食品有限公司 | Preparation method for red bamboo liquid |
| CN104041905A (en) * | 2014-06-27 | 2014-09-17 | 广西健美乐食品有限公司 | Method for preparing bambusa chungii liquid |
| CN104013065B (en) * | 2014-06-27 | 2015-10-14 | 广西健美乐食品有限公司 | A kind of preparation method of red bamboo liquid |
| CN104041905B (en) * | 2014-06-27 | 2016-03-09 | 广西健美乐食品有限公司 | A kind of preparation method of red bamboo liquid |
| CN108403978A (en) * | 2018-05-29 | 2018-08-17 | 浙江竹孝宝生物科技有限公司 | The preparation method of bamboo extractive |
| CN114052152A (en) * | 2021-09-28 | 2022-02-18 | 海南黎草纪新生物科技有限公司 | Extraction method of plant micromolecular water |
| CN114052152B (en) * | 2021-09-28 | 2023-05-09 | 海南黎草纪新生物科技有限公司 | Extraction method of plant small molecular water |
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