CN1488364A - Method for treating aloe gel, aloe leaf gel and aloe hide glue and aloe polyaccharide obtained thereof - Google Patents
Method for treating aloe gel, aloe leaf gel and aloe hide glue and aloe polyaccharide obtained thereof Download PDFInfo
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Abstract
The invention is a method to use enzymolysis to process aloe gel, aloe full-leaf gel and aloe peel gel, and its aloe amylase product. The method includes: make enzymolysis reaction, vacuum condensation, and solid-liquid separation to produce aloe condensed clear liquid, and then freeze-dry the condensed liquid to make aloe freeze-dried powder; acetylize the clear liquid, and then wash by alcohol and acetone to freeze-dry to make into primary aloe amylase product; make filter film separation and condensation, and solid-liquid separation on the condensed clear liquid, wash by alcohol and acetone, and then freeze-dry to make into second-level aloe amylase product; make column chromography purification on the condensed product obtained by filter-film separation, and then freeze-dry to make into first-level aloe amylase product. Solid-liquid separation obtains solid wet by-product, and the filter-film separation obtains liquid by-product.
Description
(1) technical field the present invention relates to a kind of method of handling Aloe gel, specifically be a kind ofly to destroy the viscosity of Aloe gel, relend and help the vacuum concentration technology to realize other Aloe bio-active products that from Aloe gel, aloe full leaf gel and Aloe hide glue, extract the Aloe polysaccharide product and prepare fast with this method by enzyme digestion reaction (enzymolysis).
(2) contain multiple active ingredient in the background technology aloe leaf, as tens kinds of Aloe bioactive substances such as high activity Aloe polysaccharide, Aloe anthraquinone derivative, Aloe cupreol, Aloe daucosterols, the effect that they have adjusting and stimulate the human immune system, thereby, it be widely used for human body diseases prevention, cure the disease, be mainly used in the healing of antiinflammatory, antiviral, antitumor, relieving constipation, promotion wound etc.It can promote the regeneration of human body skin and human body skin to preserve moisture.Therefore be widely used in the cosmetics.Aloe polysaccharide and other Aloe bioactive substance have extensive use in the world of medicine and cosmetics circle, are subject to people's attention always.Since the beginning of the eighties in last century, many in the world Aloe experts carry out fruitful research to Aloe polysaccharide and other Aloe bioactive substance, comprise the research of extracting Aloe polysaccharide and other Aloe bioactive substance from aloe leaf; Aloe polysaccharide and other Aloe bioactive substance are used for the research of medicine and cosmetics etc.Aspect the research of extracting the gelatinous polysaccharide of aloe method, the most fruitful is the card Islington laboratory (Carrington lab inc.) of California, USA.At the end of the eighties, this laboratory proposes to extract Aloe polysaccharide with alcohol precipitation.The Aloe polysaccharide that extracts with this method exists the cost height, shortcomings such as the rehydration difference of Aloe polysaccharide goods.At the end of the nineties, this laboratory company proposes again with micro-pore-film filtration, super membrane filtration, column chromatography, dialysis, high speed centrifugation (100,000 go to 150,000 commentaries on classics), and gamma ray radiation is extracted Aloe polysaccharide and other Aloe bioactive substance.Thereby the purity of the polysaccharide that extracts and the rehydration of polysaccharide have been improved.The principle that card Islington laboratory adopts the filter membrane isolation technics to extract gelatinous polysaccharide of aloe and other Aloe bioactive substance institute foundation is the molecular weight distribution of other Aloe bioactive substance in Aloe polysaccharide molecular weight distribution and the Aloe gel, select for use specific fenestra that gel is filtered, other Aloe bioactive substance of filtering.A small amount of other Aloe bioactive substance is trapped in the concentrated glue that contains polysaccharide, because the viscosity of Aloe gel is too big, must adopt the ultrahigh speed centrifuge could remove other Aloe bioactive substance in the aloe extract effectively.At last centrifugal clear liquid is carried out lyophilization and make the Aloe polysaccharide lyophilized powder.The inventor Qiu Zhihua of U.S. Westminster CO. proposes to add the cellulase of 0.5 gram to 2.5 grams in 216 liters Aloe kind is the Aloe gel of vera, in temperature is under 2～2.5 hours the condition of 25 degree reactions, carry out enzyme digestion reaction, decompose the cellulose in the Aloe gel, made things convenient for the extraction Aloe polysaccharide.But it is Aloe polysaccharide more than 50000 that this method only is used for extracting the Aloe gel molecular weight of U.S. Aloe vera product clock.(seeing CN patent1034313A, CN patent 86104468, U.S patent 5902796, U.S patent 6133440, U.S patent 6001572, U.S patent 6117847, U.S patent6271214, US patent 6436679, US patent 6423328).But, because the viscosity of Aloe gel is big, it is separated from one another or be difficult in containing the complicated Aloe gel of tens kinds of Aloe bioactive substances with these useful compositions to adopt the breadboard extraction process of card Islington to be difficult to, Aloe polysaccharide is carried out industrialized extraction, purification, extract Aloe polysaccharide and other bioactive substance even adopt this technology to carry out industrialization, its equipment investment is very expensive, production cost is very high, complicated operation, the response rate of Aloe polysaccharide is low, and is difficult to realize the big commercial production of Aloe polysaccharide.Owing in the aloe leaf that China produced, contain impurity polysaccharide such as a small amount of pectin and small quantity of dextran, therefore, the extraction Aloe polysaccharide method of Westminster CO. is not suitable for the Aloe that produces from China and comprises the processing technique of extracting Aloe polysaccharide the Aloe vera L.var. chinensis(Haw.)Berg., is not suitable for from aloe full leaf gel, Aloe hide glue yet and extracts Aloe polysaccharide.
In the method for handling Aloe gel, the existing two kinds of methods of destroying Aloe gel viscosity of China, the method that one, China Patent No. CN1173354A, CN1059810C provided, this method proposition addition in Aloe gel reaches the pectase of 0.1～3.0% (1000～30000 gram/ton), the enzyme digestion reaction condition is PH:3～5, normal-temperature reaction: 2～20 days, at 40～50 ℃, sterilized 30～50 minutes.Because most important bioactive ingredients is an Aloe polysaccharide in the Aloe gel, it is the polymeric polymerization polysaccharide of about ratio by 20 mannose and 1 galactose, and pectase is the catabolic enzyme of galactan, handle Aloe gel with so a large amount of pectases, after enzyme digestion reaction, its result must make high-molecular weightly to be had very the Aloe polysaccharide of strong biological activity and will be broken down into and not have bioactive micromolecule oligosaccharide (molecular weight does not have biological activity less than 8000 Aloe polysaccharide) and monosaccharide.Therefore, though this method can obviously reduce the viscosity of Aloe gel, nonsensical in process for extracting aloinose.Two, the method that provided of China Patent No. CN1216232A, the method for this processing Aloe gel is: under acid condition (PH:3.2～4.2), contain H in Aloe gel
2O
2Situation under, 70 ℃～90 ℃ of high temperature were handled Aloe gel about 3 hours, consequently Aloe gel is under high temperature like this, Aloe polysaccharide wherein can be oxidized to oligomeric alditol, makes Aloe polysaccharide lose biological activity.Second method has also lost the meaning of extracting Aloe polysaccharide from Aloe gel.Therefore, these two kinds of methods can not be used for the preparation technology of Aloe polysaccharide.
(3) summary of the invention the invention provides a kind of processing Aloe gel; the aloe full leaf gel; the method of Aloe hide glue and Aloe gel thereof from having handled; the aloe full leaf gel; extract the technology of Aloe polysaccharide in the Aloe hide glue; it is Aloe polysaccharide more than 5000 that this technology can be extracted molecular weight; the viscosity that this technology can be destroyed Aloe gel has made things convenient for the preparation of Aloe polysaccharide again; protect the structure of Aloe polysaccharide constant to greatest extent; be that the biological activity of Aloe polysaccharide is constant in the Aloe gel; and it is few to produce the required equipment investment of Aloe polysaccharide; constant product quality; purity improves, and realizes industrialization easily.Principle of the present invention is: adopt to add acid protease, how active 1,4 beta-glucanase Ultraflo L, compound pectinase viscozyrae L in Aloe gel, aloe full leaf gel, Aloe hide glue, and control enzyme digestion reaction, prevent from when vacuum concentration, to produce foam, influence the operation of vacuum concentration.Enzyme digestion reaction can make the viscosity of thick Aloe gel, thick aloe full leaf gel and thick Aloe hide glue drop to about 10mpa.s～2mpa.s from 500mpa.s～20mpa.s, and is close with the viscosity 1.45mpa.s of water.Simultaneously protein is decomposed into micromolecule polypeptide and aminoacid, beta glucan is decomposed into glucose, pectin is decomposed into galactose, glycoside is decomposed into the enzyme cleavage reaction product of heteropolysaccharide such as glucose and glycoside unit.Behind enzyme digestion reaction, reuse lifting film thin film vacuum concentration technology is to low-viscosity Aloe gel, the aloe full leaf gel, the Aloe hide glue carries out vacuum concentration, the high temperature of moment (a few second in second to tens) between diakinesis because when concentrating the tube wall film forming, can kill the activity of various enzymes, thereby reach the effect of enzyme denaturing, then concentrated solution is cooled off, carry out rotational flow separation again, remove most of solid by-product, the Aloe for preparing different cycles of concentration concentrates clear liquid, and aloe extract is behind activated carbon decolorizing, and the Aloe that sterilization packaging is made different cycles of concentration concentrates the clear liquid product, the Aloe of having decoloured is concentrated clear liquid carry out lyophilization, make the aloe frozen-dried powder product.The Aloe polysaccharide that the Aloe of having decoloured is concentrated in the clear liquid carries out the acetylation processing, replenishes the acetyl group in the Aloe polysaccharide molecule in good time, thereby has improved the biological activity of Aloe polysaccharide.Reuse ethanol, acetone solvent concentrate clear liquid to Aloe and wash, and flush away residues in other Aloe bioactive substance (comprising aminoacid, polypeptide, oligosaccharide, monosaccharide etc.) in the concentrated clear liquid of Aloe, obtains Aloe polysaccharide and concentrates clear liquid.At last, this Aloe is concentrated clear liquid carry out lyophilization, made elementary Aloe polysaccharide product.
The elementary Aloe polysaccharide product of above-mentioned preparation technology is united high secondary Aloe polysaccharide product and other Aloe bioactive substance of use preparation purity with the filter membrane isolation technics.At first, thick Aloe gel, thick aloe full leaf gel and thick Aloe hide glue are carried out enzyme digestion reaction, lifting film thin film vacuum concentration carries out rotational flow separation and centrifugalize again and removes solid wet feed by-product, obtains liquid elementary aloe extract.Dilute with deionized water then; further the Aloe gel clear liquid is carried out filtering and concentrating through the filter membrane piece-rate system that micro-filtration membrane, ultrafilter membrane are formed; solid content reaches about 4% in the concentrated clear liquid of Aloe; carry out solid-liquid separation again; obtain solid wet feed by-product and Aloe and concentrate the clear liquid product, the Aloe polysaccharide that Aloe is concentrated in the clear liquid carries out the acetylation processing again.Concentrate clear liquid with ethanol, washing with acetone Aloe then.At last, the Aloe that crosses through acetylation and ethanol, washing with acetone is concentrated clear liquid carry out lyophilisation, obtain high activity secondary Aloe polysaccharide product.
The elementary Aloe polysaccharide product of above-mentioned preparation technology with filter membrane isolation technics, column chromatography technology joint operation, is further improved the purity of Aloe polysaccharide, preparation one-level Aloe polysaccharide product.At first, thick Aloe gel, thick aloe full leaf gel and thick Aloe hide glue are carried out enzyme digestion reaction, vacuum concentration, rotational flow separation, filter membrane to be separated concentrated, again with the column chromatography technology joint operation, adopt macroporous resin column and gel chromatographic columns columns in series chromatography, it is concentrated that the eluent that column chromatography is obtained carries out ultrafilter membrane then, impurity such as flush away sodium acetate obtain Aloe and concentrate clear liquid.The Aloe polysaccharide that Aloe is concentrated in the clear liquid carries out acetylation again, at last acetylizad Aloe is concentrated clear liquid and carries out lyophilization, preparation one-level Aloe polysaccharide product.
The method of processing Aloe gel provided by the invention comprises that the how active 1,4 beta-glucanase Uitrafio L of adding, acid protease, compound pectinase Viscozyme L carry out enzymolysis processing in Aloe gel, aloe full leaf gel, Aloe hide glue.
The method of processing Aloe gel provided by the invention, aloe full leaf gel, Aloe hide glue, carry out enzyme digestion reaction under the following conditions:
Acid protease addition: 5～20 gram/tons;
Many active 1,4 beta-glucanase additions: 6～30 gram/tons;
Compound pectinase viscozyme L addition 3-15 gram/ton
Temperature: 20～60 ℃;
Time: 1～10 hour;
PH：3.0～5.5。
The method of processing Aloe gel provided by the invention, enzyme digestion reaction are preferably carried out under the following conditions:
Acid protease addition: 9～15 gram/tons;
Many active 1,4 beta-glucanase additions: 10～25 gram/tons;
Compound pectinase viscozyme L addition 3-10 gram/ton;
Temperature: 30～50 ℃;
Time: 2～5 hours;
PH：4.0～5.0。
Processing Aloe gel provided by the invention, reed can full leaf gels, the method for Aloe hide glue, preferably the Aloe gel through enzyme digestion reaction carried out solid-liquid separation, vacuum concentration and solid-liquid separation for the second time, obtain elementary Aloe product.
The method of processing Aloe gel provided by the invention, aloe full leaf gel, Aloe hide glue, preferably to carry out separating of the filter membrane piece-rate system be made up of three road films concentrated through for the second time separating the elementary liquid shape Aloe product that obtained, said three road films, one, two, three road films are respectively 110～300KDa, 10～100KDa, 2～8KDa, obtain secondary Aloe product.
The method of processing Aloe gel provided by the invention, preferably the secondary liquid shape Aloe product that the filter membrane piece-rate system of forming via three road films is handled carry out the column chromatography processing, said chromatographic column is macroporous resin column or agarose column or polyacrylamide post or Superrose post, chromatographic solution PH is acetic acid-sodium acetate solution of 0.05～0.5mol of 4.5～5.0, obtains one-level Aloe product.
Aloe gel behind enzyme digestion reaction, aloe full leaf gel and Aloe hide glue, because viscosity reduces greatly, be very easy to and from these Aloe gels, extract Aloe polysaccharide and other Aloe bioactive substance, also be suitable for and from aloe full leaf gel and Aloe hide glue, extract Aloe polysaccharide and other Aloe bioactive substance.At first the impurity content of aloe full leaf gel, Aloe hide glue is higher than Aloe gel, when these two kinds of gels being carried out the process operation of enzyme decomposition reaction extraction Aloe polysaccharide and other Aloe bioactive substance, must increase the consumption of enzyme, increase the number of times that concentrates clear liquid with ethanol and washing with acetone Aloe, the purity of prepared Aloe polysaccharide is low, primary product purity is about 40%, and secondary product purity is about 65%, first class product purity about 95%.Secondly, the polysaccharide molecular weight of Aloe gel distributes greater than the Aloe hide glue, about 1 order of magnitude.And the molecular weight distribution of gelatinous polysaccharide of aloe is stable, and the Aloe polysaccharide molecular weight distribution in the hide glue can be subjected to the variation of weather, environment and fluctuate.But the processing process of these three kinds of gel extraction Aloe polysaccharides is identical.The following Aloe gel of this paper is as the abbreviation of Aloe gel, aloe full leaf gel, these three kinds of gels of Aloe hide glue.
(4) description of drawings
Fig. 1: enzyme digestion reaction is handled Aloe gel and the elementary Aloe polysaccharide product process flow of a cycles prepare sketch map
Fig. 1 explanation: enzyme digestion reaction is handled Aloe gel and the elementary Aloe polysaccharide product process flow of a cycles prepare sketch map.
To Aloe gel, the aloe full leaf gel, the Aloe hide glue adds enzyme preparation and carries out enzyme digestion reaction, destroy the viscosity of Aloe gel, carry out solid-liquid separation again, vacuum concentration, make aloe extract, aloe extract is through lyophilization, make no bleaching aloe frozen-dried powder product, aloe extract is carried out solid-liquid separation, it is first rotational flow separation, again the underflow that rotational flow separation obtained is carried out centrifugalize, the Aloe that rotational flow separation and centrifugalize are obtained concentrates clear liquid and merges, and the Aloe that obtains different multiples concentrates the clear liquid product, to concentrating clear liquid reuse ethanol behind acetyl, washing with acetone is made elementary Aloe polysaccharide product through the freeze dryer lyophilization then.Solid-liquid separation also obtains solid wet feed by-product.
This technological process can obtain following product or by-product:
1. aloe frozen-dried powder product;
2. the aloe extract product of different cycles of concentration;
3. elementary Aloe polysaccharide product;
4. solid wet feed by-product.
Fig. 2: enzyme digestion reaction is handled Aloe gel and a cycles prepare secondary Aloe polysaccharide product process flow sketch map
Fig. 2 explanation: enzyme digestion reaction is handled Aloe gel and a cycles prepare secondary Aloe polysaccharide product process flow sketch map.
Aloe gel, aloe full leaf gel, Aloe hide glue are added enzyme preparation; carry out enzyme digestion reaction; carry out solid-liquid separation again; carry out vacuum concentration then; make aloe extract; aloe extract is carried out solid-liquid separation; again the Aloe gel that obtains is concentrated clear liquid and carry out microfiltration, ultrafiltration three road filter membrane filtering and concentrating; aloe extract after concentrating carries out solid-liquid separation; obtain Aloe and concentrate the clear liquid product, Aloe concentrates clear liquid after acetylation, reuse ethanol, washing with acetone; use the freeze dryer lyophilization then, make secondary Aloe polysaccharide product.Solid-liquid separation also obtains solid wet feed by-product, and the filter membrane separation also obtains the liquid by-product.
This technological process can obtain following product or by-product:
1. secondary Aloe polysaccharide product;
2. the Aloe of different cycles of concentration concentrates the clear liquid product;
3. solid wet feed by-product;
4. the isolating liquid by-product of filter membrane.
Fig. 3: enzyme digestion reaction is handled Aloe gel and a cycles prepare one-level Aloe polysaccharide product process flow sketch map
Fig. 3 explanation: enzyme digestion reaction is handled Aloe gel and a cycles prepare one-level Aloe polysaccharide product process flow sketch map.
Add enzyme preparation to Aloe gel, aloe full leaf gel, Aloe hide glue, carry out enzyme digestion reaction, solid-liquid separation, carry out vacuum concentration and solid-liquid separation again, again the Aloe gel that obtains is concentrated that clear liquid carries out microfiltration, ultrafiltration three road filter membranes concentrate; Aloe extract after concentrating carries out solid-liquid separation again; Aloe is concentrated clear liquid carry out column chromatography again, the column chromatography permeate concentrates through 2KDa filter membrane membrane filtration, after carrying out acetylation again and handling, concentrates clear liquid through the freeze dryer lyophilization, makes one-level Aloe polysaccharide lyophilized powder product.And solid-liquid separation also obtains solid wet feed by-product, and the filter membrane separation also obtains the liquid by-product.This technological process can obtain following product or by-product:
1. one-level Aloe polysaccharide product;
2. solid wet feed by-product;
3. the isolating liquid by-product of filter membrane
(5) specific embodiment
Specific embodiment of using the Aloe gel of handling through enzyme digestion reaction to do the different Aloe polysaccharide product of feedstock production purity grade below illustrates the specific embodiment of the inventive method and the beneficial effect of acquisition.
One, the preparation technology of elementary Aloe polysaccharide product
Embodiment one:
Enzyme digestion reaction, lifting film thin film vacuum concentration technology are extracted from Aloe gel and prepared Aloe polysaccharide and other Aloe bioactive substance, technological process comprises the following steps:
Step 1: low temperature prepares thick Aloe gel
Summer the fine day Aloe Lao Ye that gathers from the land for growing field crops, clean with clear water immediately, hydrogen peroxide sterilization, and send into immediately in-10 ℃～0 the freezer and deposited 6～12 hours, make the leaf temperature drop low, reach 0 ℃, scrape trimming thorn, top then, the peeling frictioning, it is broken that adhesive tape is put into the colloid mill hinge, cut with scissors the broken thick glue of Aloe after evenly and squeeze the juice, make thick Aloe gel through squeezer.Unpeeled blade is made thick aloe full leaf gel, goes the leaf leather behind the gel to become thick Aloe hide glue.
Step 2: thick Aloe gel is carried out enzyme digestion reaction
In thick Aloe gel, add how active 1,4 beta-glucanase Ultraflo L preparation 20 gram/tons, acid protease 15 gram/tons, compound pectinase viscozyme L 5 gram/tons, and Aloe gel heated to 40 ℃～50 ℃, insulation enzyme digestion reaction 3 hours, then this thick Aloe gel pump in the storage tank of rotational flow separation machine.
Step 3: thick Aloe gel is carried out solid-liquid separation with rotational flow separation machine and centrifugal separator
From the storage tank of rotational flow separation machine, thick Aloe gel is pumped into and carries out solid-liquid separation in the rotational flow separation machine, the effusive Aloe gel clear liquid of purified liquor outlet pumps into and concentrates in the clear liquid storage tank, the end opening of rotational flow separation machine is emitted the solids underflow, the reuse centrifugal separator carries out solid-liquid separation to the solids underflow, obtains centrifugal Aloe clear liquid and solid wet feed by-product.The Aloe clear liquid that centrifugal Aloe clear liquid pumps in the clear liquid storage tank with rotational flow separation merges.Obtain solid wet feed by-product simultaneously, and leave in the solid wet feed by-product storage tank of centrifugal separator.Above-mentioned Aloe clear liquid is the Aloe gel clear liquid.
Step 4: Aloe gel is carried out lifting film thin film vacuum concentration, and thickening temperature is 55 ℃～65 ℃, vacuum is-and 0.096Mpa～-0.098Mpa.The Aloe gel that obtains is concentrated clear liquid to be incorporated into to concentrate in the clear liquid storage tank and is cooled to room temperature.
Step 5: aloe gel concentrate is carried out solid-liquid separation, 3 operate set by step, obtain Aloe gel and concentrate clear liquid and solid wet feed by-product.
Step 6: the operation repeated multiple times of step 4, step 5, concentrate clear liquid up to the Aloe gel clear liquid that step 3 obtained being concentrated into 50 times of Aloes, the active carbon of adding 7% to 12% decolours in the Aloe gel clear liquid.Packing is sterilized then, and the decolored aloe gel of making different cycles of concentration concentrates the clear liquid product.The concentrated clear liquid of decolored aloe gel is carried out lyophilization make decolored aloe lyophilized powder product
Step 7:50 times of Aloe concentrates the acetylation processing of Aloe polysaccharide in the clear liquid.
Concentrate slowly to drip in the clear liquid to the Aloe of 50 times of decolourings and account for 1/40 the acetic anhydride that concentrates the clear liquid gross weight, pump stream stirred normal-temperature reaction 12 hours.
Step 8: 50 times of Aloe gels that are acetylation are concentrated clear liquid use the washing with alcohol secondary earlier, the reuse washing with acetone once.
Step 9: the Aloe after cleaning concentrates clear liquid and makes elementary Aloe polysaccharide lyophilized powder through the freezer dryer lyophilizing.
Step 10: the Quality Identification of elementary Aloe polysaccharide product:
Method by light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or QB/T2489-2000 is carried out quality testing to elementary gelatinous polysaccharide of aloe product.Its product quality indicator is as follows:
Moisture: 3.0%,
Polyoses content: 53%,
Aloin content: 205mg/kg,
Hydrargyrum, lead, arsenic etc. do not detect.
Identify do not have sensitizing factor and toxicity through the medicine assessment center.
*The Aloe of different multiples concentrates the quality index of clear liquid product and the quality index of aloe frozen-dried powder product meets the Chinese people fully
*Other prescription of the listed level of light industry standard QB/T2488-2000 of republic or QB/T2489-2000, this paper is unlisted.
*The Aloe polysaccharide quality index of aloe full leaf gel, Aloe hide glue is unlisted
Table 1: prepare elementary Aloe polysaccharide product embodiments
| Embodiment | The enzyme digestion reaction of Aloe gel | Lifting film thin film vacuum concentration | Product quality | ||||||||
| Many active 1,4 beta-glucanase Ultraflo L g/T | Acid protease g/T | Temperature ℃ | Acid-base value PH | Time hour | Vacuum Mpa | Temperature ℃ | Concentration time | Moisture content % | Polyoses content % | Aloin content mg/kg | |
| ????20 | ??12 | ?40～50 | ?4～5 | ?3 | -0.096～- 0.098 | ?55～65 | Hurry up | ??3 | ??55 | ??210 | |
| ????25 | ??9 | ?40～50 | ?4～5 | ?3 | -0.096～- 0.098 | ?55-65 | Hurry up | ??3 | ??54 | ??212 | |
| Four | ????20 | ??12 | ?40～50 | ?4～5 | ?3 | Rise the film vacuum concentration | Slowly | ??3.5 | ??50 | ??208 | |
| -0.096～- 0.098 | ?55～65 | ||||||||||
| Five | ????20 | ??12 | ?40～50 | ?4～5 | ?3 | The falling liquid film vacuum concentration | Slowly | ??3.5 | ??49 | ??215 | |
| -0.096～- 0.098 | ?55～65 | ||||||||||
| Six | ????20 | ??12 | ?40～50 | ?4～5 | ?3 | Spherical vacuum concentration | Very slow | ??3.5 | ??40 | ??250 | |
| -0.096～- 0.098 | ?55～65 | ||||||||||
*Preparation technology is identical with embodiment one.
*Compound pectinase viscozyme L addition in the foregoing description: 5 gram/tons.
*The Aloe of different multiples concentrates the quality index of clear liquid product and the quality index of aloe frozen-dried powder product meets the light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or QB/T2489 2000 listed other prescriptions of level fully, and this paper is unlisted.Because the solid wet feed by-product that obtains can be used as the raw material of producing other aloe products, so the quality index of the unlisted solid wet feed of this paper by-product.
Two, the preparation technology of secondary Aloe polysaccharide product
Embodiment seven:
Enzyme digestion reaction, lifting film thin film vacuum concentration and filter membrane isolation technics joint operation are extracted Aloe polysaccharide from Aloe gel, preparation secondary Aloe polysaccharide product, technological process comprises the following steps:
Step 1: step 1, step 2, step 3, step 4, step 5, the step 6 of pressing embodiment one prepare 50 times the concentrated clear liquid of Aloe, with deionized water 50 times of Aloes are concentrated clear liquid and dilute, and the Aloe that obtains 2 times concentrates clear liquid.
Step 2: the formation of filter membrane piece-rate system constitutes the first road film by the 200KDa micro-filtration membrane, and the 50KDa ultrafilter membrane constitutes the second, and the 8K ultrafilter membrane constitutes the 3rd road film.2 times of Aloes concentrate clear liquid through the first road membrane filtration, 2 times of Aloes are concentrated clear liquid be divided into two parts, and a part is dammed to circulate by the first road film and is concentrated into the concentrated solution of solid content 4%.Another part sees through the first road film and enters the second road film, and the second road membrane filtration concentrates the first road membrane filtration permeate, and the circulation of damming is concentrated into the concentrated solution of solid content 4%.
The 3rd road membrance concentration second road membrane filtration permeate, the circulation of damming is concentrated into the concentrated solution of solid content 4%, and the 3rd road membrane filtration permeate is the liquid by-product.
Step 3: the solid-liquid separation of filter membrane being separated the aloe extract that obtains.To carrying out rotational flow separation and centrifugalize respectively, obtain Aloe and concentrate clear liquid and solid wet feed by-product through the spissated aloe extract of three road filter membranes.
Step 4: the Aloe polysaccharide that filter membrane is separated in the concentrated clear liquid of back Aloe carries out the acetylation processing.The Aloe that obtains in the step 3 is concentrated clear liquid merge solid content about 4%.Concentrate the slow acetic anhydride that accounts for concentrated clear liquid gross weight 1/40 that drips in the clear liquid to Aloe, pump stream stirred normal-temperature reaction 12 hours.
Step 5: acetylizad Aloe gel concentrates clear liquid and uses the washing with alcohol secondary earlier, and the reuse washing with acetone once.
Step 6: the Aloe after cleaning concentrates clear liquid and makes secondary Aloe polysaccharide lyophilized powder through the freezer dryer lyophilizing.
Step 7: the Quality Identification of secondary gelatinous polysaccharide of aloe product.
Method by light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or QB/T2489-2000 is carried out quality testing to secondary gelatinous polysaccharide of aloe product.Its product quality indicator is as follows:
Outward appearance: the slight red flakey solid of white flakey solid or band,
Moisture: 2.5%,
Polyoses content: 70%,
Aloin: 65mg/kg,
Hydrargyrum, lead, arsenic are all undetected.
Through the medicine assessment, there are not toxicity and sensitizing factor.
*The Aloe of different multiples concentrates the quality index of clear liquid product and the quality index of aloe frozen-dried powder product meets the light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or other prescription of the listed level of QB/T2489-2000 fully, and this paper is unlisted.Because the liquid by-product, the solid wet feed by-product that obtain can be used as the raw material of producing other aloe products, so the quality index of the unlisted liquid by-product of this paper, solid wet feed by-product.
*The quality index of the product that aloe full leaf gel, Aloe hide glue are made is unlisted.
Table 2: preparation secondary Aloe polysaccharide product embodiments
| Embodiment | 50 times of Aloes concentrate clear liquid preparation technology | Membrane filtration concentrates preparation technology's film model | Product quality | |||
| Membrane filtration road number | Per pass film model KDa | Moisture content % | Polyoses content % | Aloin content mg/kg | ||
| Eight | Press embodiment one technological operation | Three | ??300??100??8 | ??2.5 | ????75 | ????60 |
| Nine | Press embodiment one technological operation | Three | ??200???????8 | ??2.5 | ????65 | ????70 |
| Ten | Press embodiment two technological operations | Three | ??200??50???8 | ??2.5 | ????74 | ????68 |
| 11 | Press embodiment two technological operations | Three | ??300??100??8 | ??2.5 | ????75 | ????62 |
| Embodiment | 50 times of Aloes concentrate clear liquid preparation technology | Membrane filtration concentrates preparation technology's film model | Product quality | |||
| Membrane filtration road number | Per pass film model KDa | Moisture content % | Polyoses content % | Aloin content mg/kg | ||
| 12 | Press embodiment two technological operations | Two | ??200??????8 | ??2.5 | ????70 | ????75 |
| 13 | Press embodiment three technological operations | Two | ??200??50??8 | ??2.5 | ????75 | ????58 |
| 14 | Press embodiment three technological operations | Two | ??300??100?8 | ??2.5 | ????73 | ????60 |
| 15 | Press embodiment three technological operations | Two | ??200??????8 | ??2.5 | ????72 | ????76 |
*Preparation technology is identical with embodiment seven.
*The Aloe of different multiples concentrates the quality index of clear liquid product and the quality index of aloe frozen-dried powder product meets the light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or other prescription of the listed level of QB/T2489-2000 fully, and this paper is unlisted.Because the liquid by-product, the solid wet feed by-product that obtain can be used as the raw material of producing other aloe products, so the quality index of the unlisted liquid by-product of this paper, solid wet feed by-product.
Three, the preparation technology of one-level Aloe polysaccharide product
Embodiment 16:
Enzyme digestion reaction, vacuum concentration, filter membrane isolation technics and column chromatography technology joint operation are extracted Aloe polysaccharide from Aloe gel, aloe full leaf gel and Aloe hide glue, preparation one-level Aloe polysaccharide product, technological process comprises the following steps:
Step 1: through enzyme digestion reaction, 50 times of Aloes of vacuum concentration concentrate the preparation of clear liquid and operate by step 1, step 2 step 3, step 4, step 5, the step 6 of embodiment one.
Step 2: the concentrated clear liquid of Aloe gel carries out the filter membrane separation again and concentrates, and the Aloe gel that obtains solid content 4% concentrates clear liquid, presses step 2, the step 3 of embodiment seven and operates.
Step 3: the filter membrane of solid content 4% is concentrated clear liquid dilute one times, the preparative column chromatographic solution with acetic acid-sodium acetate 0.1mol solution of PH=4.5.
Step 4: the macroporous resin chromatographic column is connected with Superrose chromatographic column (perhaps agarose chromatography post), carries out column chromatography.
Step 5: mobile phase is acetic acid-sodium acetate 0.1mol, PH=4.5.
Step 6: the concentrating of eluent.Use the 2KDa ultrafilter membrane to be concentrated into the concentrated clear liquid of solid content 4%.
Step 7: to the column chromatography eluent and after filtration in the clear liquid of membrance concentration Aloe polysaccharide carry out acetylation and handle.Press step 7 operation of embodiment one.
Step 8: the Aloe that has been acetylation is concentrated clear liquid carry out vacuum lyophilization, make one-level Aloe polysaccharide lyophilized powder product.
Step 9: the Quality Identification of one-level gelatinous polysaccharide of aloe product.
Method by light industry standard QB/T2488-2000 of the People's Republic of China (PRC) or QB/T2489-2000 is carried out quality testing to one-level gelatinous polysaccharide of aloe product.Its product quality indicator is as follows:
Outward appearance: white flakey solid,
Water content: 2%,
Polyoses content: 98%,
Aloin content: 1.2mg/kg.
Noxious substances such as no hydrargyrum, lead, arsenic, no sensitizing factor.
Table 3: preparation one-level Aloe polysaccharide product embodiments
| Embodiment | 50 times of Aloes concentrate covers preparation technology | The filter membrane separation concentrated solution prepares reference | The further purification technique chromatographic column of column chromatography model | Product quality | ||
| Moisture content % | Polyoses content % | Aloin content mg/kg | ||||
| 17 | Press embodiment one operation | Embodiment seven | The Superrose post | 2 | ????97 | ????0.7 |
| 18 | Press embodiment one operation | Embodiment seven | Macroporous resin column+agarose column | 2 | ????98 | ????0.6 |
| 19 | Press embodiment one operation | Embodiment seven | Agarose column | 2 | ????97 | ????0.8 |
| 20 | Press embodiment one operation | Embodiment seven | The polyacrylamide post | 2 | ????95 | ????1.0 |
| 21 | Press embodiment two operations | Embodiment seven | Macroporous resin column+Superrose post | 2 | ????97 | ????0.8 |
| 22 | Press embodiment two operations | Embodiment seven | The Superrose post | 2 | ????96 | ????0.7 |
| 23 | Press embodiment two operations | Embodiment seven | Macroporous resin column+agarose column | 2 | ????98 | ????0.5 |
| 24 | Press embodiment two operations | Embodiment seven | Agarose column | 2 | ????97 | ????0.6 |
| 25 | Press embodiment two operations | Embodiment seven | The polyacrylamide post | 2 | ????96 | ????1.0 |
| 26 | Press embodiment three operations | Embodiment seven | Macroporous resin column+Superrose post | 2 | ????98 | ????0.5 |
| 27 | Press embodiment three operations | Embodiment seven | The Superrose post | 2 | ????95 | ????0.8 |
| 28 | Press embodiment three operations | Embodiment seven | Macroporous resin column+agarose column | 2 | ????98 | ????0.4 |
| 29 | Press embodiment three operations | Embodiment seven | Agarose column | 2 | ????95 | ????1.0 |
| 30 | Press embodiment three operations | Embodiment seven | The polyacrylamide post | 2 | ????96 | ????1.0 |
| Hentriaconta- | Press embodiment one operation | Embodiment eight | Macroporous resin column+Superrose post | 2 | ????97 | ????0.6 |
| 32 | Press embodiment one operation | Embodiment eight | The Superrose post | 2 | ????96 | ????0.8 |
| 33 | Press embodiment one operation | Embodiment eight | Macroporous resin column+agarose column | 2 | ????97 | ????0.7 |
| Embodiment | 50 times of aloe extract preparation technologies | The filter membrane separation concentrated solution prepares reference | The further purification technique chromatographic column of column chromatography model | Product quality | ||
| Moisture content % | Polyoses content % | Aloin content mg/kg | ||||
| 34 | Press embodiment one operation | Embodiment eight | Agarose column | 2 | ????96 | ????0.7 |
| 35 | Press embodiment one operation | Embodiment eight | The polyacrylamide post | 2 | ????95 | ????0.8 |
| 36 | Press embodiment two operations | Embodiment eight | Macroporous resin column+Superrose post | 2 | ????98 | ????0.6 |
| 37 | Press embodiment two operations | Embodiment eight | The Superrose post | 2 | ????98 | ????0.8 |
| 38 | Press embodiment two operations | Embodiment eight | Macroporous resin column+agarose column | 2 | ????98 | ????0.8 |
| 39 | Press embodiment two operations | Embodiment eight | Agarose column | 2 | ????96 | ????0.9 |
| 40 | Press embodiment two operations | Embodiment eight | The polyacrylamide post | 2 | ????95 | ????1.0 |
| 41 | Press embodiment three operations | Embodiment eight | Macroporous resin column+Superrose post | 2 | ????98 | ????0.9 |
| 42 | Press embodiment three operations | Embodiment eight | The Superrose post | 2 | ????95 | ????1.0 |
| 43 | Press embodiment three operations | Embodiment eight | Macroporous resin column+agarose column | 2 | ????97 | ????0.6 |
| 44 | Press embodiment three operations | Embodiment eight | Agarose column | 2 | ????95 | ????1.2 |
| 45 | Press embodiment three operations | Embodiment eight | The polyacrylamide post | 2 | ????95 | ????0.9 |
*Preparation technology is identical with embodiment 16.
*The listed product quality detection method of last table 9 is carried out set by step.
Those of ordinary skills can carry out all changes after reading description, but these changes are all within the claim protection domain that application is awaited the reply.
Claims (12)
1. method of handling Aloe gel, aloe full leaf gel and Aloe hide glue, it is characterized in that this method comprises in Aloe gel, aloe full leaf gel, Aloe hide glue adds 1,4 beta-glucanase, acid protease and pectase, carries out the processing step of enzyme digestion reaction.
2. according to the method for claim 1, it is characterized in that the condition of enzyme digestion reaction is:
Acid protease addition: 5～20 gram/tons;
1,4 beta-glucanase addition: 6～30 gram/tons;
Pectase addition: 1～15 gram/ton;
Temperature peace: 20～60 ℃;
Time: 1～10 hour;
PH：3.0～5.5。
3 methods according to claim 1 is characterized in that preferred enzyme digestion reaction condition is:
Acid protease addition: 9～15 gram/tons;
1,4 beta-glucanase addition: 10～25 gram/tons;
Pectase addition: 2～10 gram/tons;
Temperature: 30～50 ℃;
Time: 2～4 hours;
PH：4.0～5.0。
4. according to the method for claim 2, it is characterized in that Aloe gel, aloe full leaf gel, Aloe hide glue through enzyme digestion reaction are carried out solid-liquid separation, vacuum concentration and solid-liquid separation for the second time, obtain elementary Aloe product.
5. according to the method for claim 3, it is characterized in that Aloe gel, aloe full leaf gel, Aloe hide glue through enzyme digestion reaction are carried out solid-liquid separation, vacuum concentration and the solid-liquid separation second time obtain elementary Aloe product.
6. according to the method for claim 4 or 5, it is characterized in that said vacuum concentration is a lifting film thin film vacuum concentration.
7. according to the method for claim 4 or 5, it is characterized in that said must solid-liquid separation be rotational flow separation or centrifugalize, preferably rotational flow separation, the perhaps solid-liquid separation system of the two combination.
8. according to the method for claim 4, it is characterized in that the membrane filtration that the elementary liquid shape Aloe product that obtained is carried out the filter membrane piece-rate system be made up of three road films concentrates, one, two, three films of said three road films are respectively 110～300KDa, 10～100KDa, 2～8KDa, obtain secondary Aloe product.
9. according to the method for claim 5, it is characterized in that the membrane filtration that the elementary liquid shape Aloe product that obtained is carried out the filter membrane piece-rate system be made up of three road films concentrates, one, two, three films of said three road films are respectively 110～300KDa, 10～100KDa, 2～8KD.Obtain secondary Aloe product.
10. method according to Claim 8 is characterized in that the elementary or secondary liquid shape Aloe product that obtained are carried out column chromatography, and said chromatographic column is macroporous resin column and agarose column; Or poly amic acid post; Or Superrose post, can adopt the single-column chromatography, perhaps macroporous resin column respectively with its excess-three kind post in a kind of chromatographic column of connecting of making, chromatographic solution PH is acetic acid-sodium acetate solution of 0.05～0.5mol of 4.5～5.0, obtain one-level Aloe product, the Aloe product are carried out freezing doing, obtain one-level Aloe product.
11. method according to claim 9, it is characterized in that the elementary or secondary liquid shape Aloe product that obtained are carried out column chromatography, said chromatographic column is macroporous resin column or agarose column or poly amic acid post, or Superrose post, adopt the single-column chromatography, perhaps macroporous resin column respectively with its excess-three kind post in a kind of chromatographic column of connecting of making, the PH of column chromatography liquid is acetic acid-sodium acetate solution of 0.05～0.5mol of 4.5～5.0, obtains one-level Aloe product.
12. the method in above-mentioned each claim makes aloe products.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021310998A CN1488364A (en) | 2002-10-09 | 2002-10-09 | Method for treating aloe gel, aloe leaf gel and aloe hide glue and aloe polyaccharide obtained thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA021310998A CN1488364A (en) | 2002-10-09 | 2002-10-09 | Method for treating aloe gel, aloe leaf gel and aloe hide glue and aloe polyaccharide obtained thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1301718C (en) * | 2004-06-11 | 2007-02-28 | 山东农业大学 | Extraction for radix cynanchi bungei polysaccharide and oral lozenge preparation |
| CN101258922B (en) * | 2008-04-23 | 2012-05-09 | 云南元江万绿生物（集团）有限公司 | Comprehensive utilization aloe skin processing method |
| CN102824287A (en) * | 2012-09-07 | 2012-12-19 | 云南万绿生物股份有限公司 | Method for removing calcium and magnesium in aloe gel juice |
| CN104402851A (en) * | 2014-09-30 | 2015-03-11 | 江苏奇力康皮肤药业有限公司 | Method for extracting barbaloin used for cosmetic |
| CN104693315A (en) * | 2015-03-16 | 2015-06-10 | 云南万绿生物股份有限公司 | Processing method for 1000: 1 aloe gel dry powder |
| CN105076891A (en) * | 2015-09-23 | 2015-11-25 | 山东新希望六和集团有限公司 | Meat duck feed additive low in excretion and preparation method thereof |
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| CN106360718A (en) * | 2016-08-26 | 2017-02-01 | 湖州佳美生物化学制品有限公司 | Extraction technology of aloe vera gels |
| CN109731062A (en) * | 2018-11-30 | 2019-05-10 | 云南万绿生物股份有限公司 | A kind of preparation method of Large molecule active aloe frozen-dried powder |
| CN114605566A (en) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | Method for extracting aloe gel macromolecule active ingredient and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1301718C (en) * | 2004-06-11 | 2007-02-28 | 山东农业大学 | Extraction for radix cynanchi bungei polysaccharide and oral lozenge preparation |
| CN101258922B (en) * | 2008-04-23 | 2012-05-09 | 云南元江万绿生物（集团）有限公司 | Comprehensive utilization aloe skin processing method |
| CN102824287A (en) * | 2012-09-07 | 2012-12-19 | 云南万绿生物股份有限公司 | Method for removing calcium and magnesium in aloe gel juice |
| CN102824287B (en) * | 2012-09-07 | 2013-09-11 | 云南万绿生物股份有限公司 | Method for removing calcium and magnesium in aloe gel juice |
| CN104402851A (en) * | 2014-09-30 | 2015-03-11 | 江苏奇力康皮肤药业有限公司 | Method for extracting barbaloin used for cosmetic |
| CN104402851B (en) * | 2014-09-30 | 2016-08-24 | 江苏奇力康皮肤药业有限公司 | A kind of extracting method of the barbaloin for cosmetics |
| CN104693315A (en) * | 2015-03-16 | 2015-06-10 | 云南万绿生物股份有限公司 | Processing method for 1000: 1 aloe gel dry powder |
| CN105076891A (en) * | 2015-09-23 | 2015-11-25 | 山东新希望六和集团有限公司 | Meat duck feed additive low in excretion and preparation method thereof |
| CN105166538A (en) * | 2015-09-23 | 2015-12-23 | 山东新希望六和集团有限公司 | Low-emission meat duck feed and preparing method thereof |
| CN106360718A (en) * | 2016-08-26 | 2017-02-01 | 湖州佳美生物化学制品有限公司 | Extraction technology of aloe vera gels |
| CN109731062A (en) * | 2018-11-30 | 2019-05-10 | 云南万绿生物股份有限公司 | A kind of preparation method of Large molecule active aloe frozen-dried powder |
| CN114605566A (en) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | Method for extracting aloe gel macromolecule active ingredient and application thereof |
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