CN104592412A - Method for extracting chestnut polysaccharide - Google Patents
Method for extracting chestnut polysaccharide Download PDFInfo
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- CN104592412A CN104592412A CN201510091131.XA CN201510091131A CN104592412A CN 104592412 A CN104592412 A CN 104592412A CN 201510091131 A CN201510091131 A CN 201510091131A CN 104592412 A CN104592412 A CN 104592412A
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Abstract
The invention provides a method for extracting chestnut polysaccharide. The method comprises the following steps: (1), selecting fresh Chinese chestnuts, cleaning and peeling the chestnuts, crushing and drying the chestnuts, putting in a crusher to be crushed to powder, and after sieving by a 40-mesh sieve to degrease; (2), adding distilled water into the degreased chestnut powder, putting the powder in an ultrasonic cleaner to be treated for 20-28 minutes, putting the powder in a constant temperature water bath kettle at a temperature of 75-85 DEG C for extraction for 2.5 hours; (3), after the extraction is accomplished, putting an extraction liquid in a centrifugal machine to be centrifugalized and taking supernate, and adding 95% ethanol which is 4 times in volume into the supernate for alcohol precipitation overnight; (4), putting the solution after alcohol precipitation in the centrifugal machine to be centrifugalized, recovering ethanol in the upper layer, centrifugalizing, precipitating and dialyzing to freeze and dry to obtain the chestnut polysaccharide. The extraction method is low in cost, short in time consumed, high in extraction rate, high in purity, simple and easy to operate and particularly suitable for industrial production.
Description
Technical field
The present invention relates to a kind of method extracting polysaccharide, be specifically related to a kind of method extracting chestnut polysaccharide.
Background technology
Chinese chestnut, has another name called chestnut, and be the seed of tall this plant chestnut of Fagaceae, have the laudatory title of " kings of thousand fruits ", is one of world-renowned dry fruit.Fruit is rich in the Multiple components such as carbohydrate, proteins,vitamins,minerals and unsaturated fatty acids, not only has higher nutritive value, and there is strengthening spleen and nourishing stomach, the nourishing function such as vital energy benefiting and the kidney invigorating, strong muscle are invigorated blood circulation.Containing a large amount of chestnut polysaccharide in Chinese chestnut, it has pharmacological effects such as strengthening immune, antitumor, anti-oxidant, antithrombotic, antiviral, anti-mutation, hypotensive, reducing blood-fat, have good preventive and therapeutic action to hypertension, coronary heart disease, arteriosclerosis and insufficiency of the spleen, the disease such as to suffer from a deficiency of the kidney, therefore the research of chestnut polysaccharide is subject to people's attention day by day in recent years.
At present, traditional method and the shortcoming of extracting natural polysaccharide are as follows: one, water extracting alcohol method, the method is extracted polysaccharide and is not needed specific installation and cost lower security, but because the polarity of water is large, easily other water soluble component impurity lixiviates out, for follow-up separation brings difficulty, and this extraction is consuming time and extraction yield is not high yet; Its two, acid-base method, may cause the fracture of glycosidic link in polysaccharide, and cause corrosion to container in Polyose extraction, or containing other impurity in extracting solution, makes the excessive filtration difficulty of viscosity, therefore impracticable; They are three years old, supercritical fluid extraction, there is the advantages such as the activity of composition of remaining valid and no solvent residue, but due to carbohydrate compound molecular weight compared with large, hydroxyl is many, polarity is large, lower with pure carbon dioxide extraction efficiency, equipment is complicated, running cost is high, be not suitable for suitability for industrialized production; In addition, ultrasonic-assisted extraction, mainly utilize hyperacoustic cavitation and oscillating action, plant cell wall is broken, and whole rupture process completes instantaneously, not only accelerating the stripping of effective components in plants, and be conducive to the reservation of activeconstituents, but not yet reach optimum about the processing condition that chestnut polysaccharide is extracted, extraction yield does not reach desirable effect, and at present both at home and abroad about the research of chestnut polysaccharide extraction process is reported very few.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide a kind of method extracting chestnut polysaccharide, the method consuming time short, cost is low, extraction yield is high, purity is high.
In order to achieve the above object, the technical scheme that the present invention takes is:
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:20 ~ 1:30g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is after the ultrasonic cleaner process 20 ~ 28min of 150 ~ 250w, puts into the thermostat water bath lixiviate 2.5h that temperature is 75 ~ 85 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
As optimal technical scheme, in order to make extraction temperature reach optimum value, be beneficial to the extraction yield and the purity that improve polysaccharide, the middle thermostat water bath of described step (2) preferably temperature is 80 DEG C.
As optimal technical scheme, in order to make ultrasonic treatment time reach optimum value, be beneficial to the extraction yield and the purity that improve polysaccharide, in described step (2), the ultrasonic cleaner treatment time is preferably 25min.
As optimal technical scheme, in order to make ultrasonic power reach optimum value, be beneficial to the extraction yield and the purity that improve polysaccharide, in described step (2), the power of ultrasonic cleaner is preferably 200w.
As optimal technical scheme, in order to make the solid-liquid ratio of Chinese chestnut powder distilled water reach optimum value, be beneficial to the extraction yield and the purity that improve polysaccharide, in described step (2), Chinese chestnut powder and the better solid-liquid ratio of distilled water are 1:25g/ml.
Beneficial effect of the present invention: utilize ultrasonic-assisted extraction chestnut polysaccharide, cost is low, consuming time short; Take optimum extracting factor, extraction yield is high, first degreasing alcohol precipitation again, and purity of polysaccharide is high; And extracting method of the present invention is simple, easy handling, is applicable to suitability for industrialized production.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:20g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is, after the ultrasonic cleaner of 150w processes 20min, put into the thermostat water bath lixiviate 2.5h that temperature is 75 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
Embodiment 2
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:22g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is, after the ultrasonic cleaner of 180w processes 22min, put into the thermostat water bath lixiviate 2.5h that temperature is 77 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
Embodiment 3
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:25g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is, after the ultrasonic cleaner of 200w processes 24min, put into the thermostat water bath lixiviate 2.5h that temperature is 80 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
Embodiment 4
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:28g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is, after the ultrasonic cleaner of 220w processes 26min, put into the thermostat water bath lixiviate 2.5h that temperature is 82 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
Embodiment 5
Extract a method for chestnut polysaccharide, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:30g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is, after the ultrasonic cleaner of 250w processes 28min, put into the thermostat water bath lixiviate 2.5h that temperature is 85 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
It should be pointed out that above case is only the representational example of the present invention.Obviously, technical scheme of the present invention is not limited to above-described embodiment, can also have many sex change.
Claims (5)
1. extract a method for chestnut polysaccharide, it is characterized in that, comprise the following steps:
(1) select fresh Chinese chestnut cleaning peeling, broken post-drying, is placed in pulverizer and is ground into powder, carries out degreasing and obtain Chinese chestnut powder after crossing 40 mesh sieves, for subsequent use;
(2) be that 1:20 ~ 1:30g/ml adds distilled water to the Chinese chestnut powder after degreasing with solid-liquid ratio, being placed in power is after the ultrasonic cleaner process 20 ~ 28min of 150 ~ 250w, puts into the thermostat water bath lixiviate 2.5h that temperature is 75 ~ 85 DEG C;
(3) to be leached complete after, extracting solution being placed in rotating speed is get supernatant liquor after the centrifuge 15min of 4000r/min, and 95% ethanol adding 4 times of volumes in supernatant liquor carries out alcohol precipitation and spends the night;
(4) solution after being spent the night by alcohol precipitation is placed in the centrifuge 10min that rotating speed is 5000r/min, reclaims upper strata ethanol, carries out lyophilize, can obtain chestnut polysaccharide after centrifugation dialysis.
2. a kind of method extracting chestnut polysaccharide according to claim 1, is characterized in that: in described step (2), the ultrasonic cleaner treatment time is 25min.
3. a kind of method extracting chestnut polysaccharide according to claim 1, is characterized in that: in described step (2), the power of ultrasonic cleaner is 200w.
4. a kind of method extracting chestnut polysaccharide according to claim 1, is characterized in that: in described step (2), Chinese chestnut powder and distilled water solid-liquid ratio are 1:25g/ml.
5. a kind of method extracting chestnut polysaccharide according to Claims 1 to 4 any one, is characterized in that: in described step (2), the temperature of thermostat water bath is 80 DEG C.
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| CN201510091131.XA CN104592412A (en) | 2015-02-28 | 2015-02-28 | Method for extracting chestnut polysaccharide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104861085A (en) * | 2015-06-16 | 2015-08-26 | 河北大学 | Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs |
| CN106632713A (en) * | 2016-10-24 | 2017-05-10 | 澳宝化妆品（惠州）有限公司 | Method for extracting natural chestnut polysaccharides and skin cream containing natural chestnut polysaccharides |
| CN107880147A (en) * | 2017-12-26 | 2018-04-06 | 河北科技师范学院 | Chinese chestnut active anticancer polysaccharide and its isolation and purification method |
| CN109287871A (en) * | 2018-09-18 | 2019-02-01 | 青岛农业大学 | A method of animal semen quality is improved using chestnut polysaccharide |
| CN115368419A (en) * | 2022-09-26 | 2022-11-22 | 河北科技师范学院 | Chinese chestnut kernel oligosaccharide extract and extraction and purification method thereof |
| CN116425900A (en) * | 2023-05-26 | 2023-07-14 | 黄冈师范学院 | Extraction method of chestnut mushroom polysaccharide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102702376A (en) * | 2012-05-24 | 2012-10-03 | 河北科技师范学院 | Process for extracting chestnut polysaccharide by pressurized solvent extraction method |
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2015
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102702376A (en) * | 2012-05-24 | 2012-10-03 | 河北科技师范学院 | Process for extracting chestnut polysaccharide by pressurized solvent extraction method |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104861085A (en) * | 2015-06-16 | 2015-08-26 | 河北大学 | Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs |
| CN106632713A (en) * | 2016-10-24 | 2017-05-10 | 澳宝化妆品（惠州）有限公司 | Method for extracting natural chestnut polysaccharides and skin cream containing natural chestnut polysaccharides |
| CN106632713B (en) * | 2016-10-24 | 2019-06-04 | 澳宝化妆品（惠州）有限公司 | The naturally extracting method of chestnut polysaccharide and the face cream comprising the natural chestnut polysaccharide |
| CN107880147A (en) * | 2017-12-26 | 2018-04-06 | 河北科技师范学院 | Chinese chestnut active anticancer polysaccharide and its isolation and purification method |
| CN109287871A (en) * | 2018-09-18 | 2019-02-01 | 青岛农业大学 | A method of animal semen quality is improved using chestnut polysaccharide |
| CN109287871B (en) * | 2018-09-18 | 2021-12-28 | 青岛农业大学 | Method for improving animal semen quality by using Chinese chestnut polysaccharide |
| CN115368419A (en) * | 2022-09-26 | 2022-11-22 | 河北科技师范学院 | Chinese chestnut kernel oligosaccharide extract and extraction and purification method thereof |
| CN115368419B (en) * | 2022-09-26 | 2024-02-23 | 河北科技师范学院 | Chestnut kernel oligosaccharide extract and extraction and purification method thereof |
| CN116425900A (en) * | 2023-05-26 | 2023-07-14 | 黄冈师范学院 | Extraction method of chestnut mushroom polysaccharide |
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