CN102702376A - Process for extracting chestnut polysaccharide by pressurized solvent extraction method - Google Patents
Process for extracting chestnut polysaccharide by pressurized solvent extraction method Download PDFInfo
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- CN102702376A CN102702376A CN2012101637910A CN201210163791A CN102702376A CN 102702376 A CN102702376 A CN 102702376A CN 2012101637910 A CN2012101637910 A CN 2012101637910A CN 201210163791 A CN201210163791 A CN 201210163791A CN 102702376 A CN102702376 A CN 102702376A
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Abstract
The invention discloses a process for extracting chestnut polysaccharide by a pressurized solvent extraction method. The process comprises the following steps of: drying and crushing chestnuts and mixing evenly with diatomaceous earth, putting to an extraction pond, adding the diatomaceous earth for filling, connecting nitrogen, an extraction instrument and a collecting bottle device, and setting the conditions of a pressurized solvent extraction instrument: the extraction temperature is 50 DEG C to 70 DEG C, the heating time is 4min to 20min, the extraction pressure is 3MPa to 15MPa, the number of cycles is 1 to 5, the elution volume is 40% to 60%, and the nitrogen blowing time is 40-100s; filtering an extract, concentrating in vacuum to 1/4 of the volume, removing protein, and repeatedly operating until a white suspension does not exist in an interface; and adding 75% to 100% of ethanol, carrying out ethanol precipitation for 5 to 12h, and drying to obtain the chestnut polysaccharide. The process provided by the invention has the advantages of less amount of solvent, simple and fast operation, improvement on extraction efficiency, and obvious reduction in extraction cost of a single sample. Meanwhile, the biological activity of the polysaccharide substance is kept.
Description
Technical field
The invention belongs to natural product extracting technique, relate to a kind of technology of utilizing pressurized liquid extraction (APLE) method to extract the Chinese chestnut polysaccharide specifically.
Background technology
Starting material Chinese chestnut (Castanea mollissima Blume) is the Fagaceae Castanea.Chinese chestnut is multiple to be planted in hillside, extensively plants in Europe, Asia and America, is the famous-brand and high-quality dry fruit of China's tradition plantation, and the Tang Dynasty medical science famous expert Sun Simiao once said: " chestnut, the fruit of kidney also, ephrosis should be eaten it; Eat raw and control waist, pin is unsuccessful." polysaccharide is sugared naturally occurring important form, is one type of polysaccharose substance with bio-physiological activity and special nourishing function.It is active to have important biomolecules such as enhancing body immunologic function, anti-oxidant, anti-ageing, antitumor, antiviral, mutation, radioprotective and hemopoietic.
The pressurized liquid extraction technology is a kind of new method that is proposed by (1995) such as Richter.Be recommended as a kind of extracting process of standard by American National Environmental Protection Agency (EPA).The present invention adopts pressurized liquid extraction technology to compare with traditional extraction process to have solvent load few, simple to operate quick, and extraction efficiency significantly improves, and reduces the extraction expense of single product, has kept the outstanding advantages such as biological activity of polysaccharide material simultaneously.Although the pressurized liquid extraction technology is the new technology that just developed in recent years,, received the very big concern of analytical chemistry circle because it is with conspicuous characteristics.This technology is used widely in fields such as environment, medicine, food, agricultural and polymer industry.But the method for utilizing the pressurized liquid extraction technology that polysaccharide material in the Chinese chestnut is extracted is not seen the report of relevant this respect at present as yet.
Summary of the invention
The purpose of this invention is to provide a kind of technology of utilizing the pressurized liquid extraction method to extract the Chinese chestnut polysaccharide.Have few, simple to operate quick, the raising extraction efficiency of solvent load, significantly reduced the extraction expense of single product.Simultaneously, also has the outstanding advantage of biological activity that keeps polysaccharide material.
The technology of extraction Chinese chestnut polysaccharide of the present invention comprises:
1, Chinese chestnut is peeled off, get the kernel of Semen Castaneae Mollissimae oven dry, temperature is 25 ℃-50 ℃, grinds to form rice-chestnut powder with kibbler then, behind the sealing bag of packing into, puts into moisture eliminator, and is subsequent use;
2, get above-mentioned rice-chestnut powder and the zeyssatite thorough mixing is even, in the abstraction pool of packing into, and then add 1 ~ 20 Kg zeyssatite and fill up abstraction pool, connect nitrogen device, abstraction instrument and receiving flask;
3, open nitrogen and abstraction instrument switch respectively, the abstraction instrument self check is cleaned the abstraction instrument pipeline with cleaning solvent, sets preheating temperature and begins preheating;
4, pressurized liquid extraction appearance condition is set: 50 ℃-70 ℃ of extraction temperature are provided with min-20 min heat-up times 4, extraction pressure: 3 MPa-15 MPa; Cycle index is set: 1-5 time; Elution volume 40 %-60 %, nitrogen blows time 40-100 s, begins extraction;
5, after extraction was accomplished, extraction liquid got into receiving flask, and extraction liquid is filtered, and vacuum concentration to 1/4 volume is removed albumen, and repeatable operation till separation surface does not have white suspendible, adds 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, dry Chinese chestnut polysaccharide.
In the present invention, rice-chestnut powder in the said step (2) and zeyssatite are pressed 20 Kg: the mixed of 10 ~ 30 Kg.
Cleaning solvent, the extraction solvent of indication of the present invention are pure water.
Therefore, the present invention integrates through the extractive technique of above-mentioned Chinese chestnut treatment technology and polysaccharose substance, can reach polysaccharide leaching process fast and efficiently.It is few to have the solvent load of extraction, and the extraction whole process time spent is short, and extraction process is a static extracting, and the volatile matter that extracts is pure fully, reduces the ruined possibility of biological activity of polysaccharide material simultaneously.
Remarkable advantage of the present invention:
The present invention adopts full-automatic pressurized liquid extraction, and (Automated Pressurized Liquid Extraction, APLE) method is extracted the Chinese chestnut polysaccharide.The pressurized liquid extraction method is compared with traditional Suo Shi extraction, supersound extraction, microwave extracting method; Have solvent load few, simple to operate fast, improve extraction efficiency, continuity is good; Significantly reduced the extraction expense of single product; Also have health environment-friendly, level of automation height, kept the bioactive outstanding advantage of polysaccharide material simultaneously.
Embodiment
Embodiment 1
1, the kernel of Semen Castaneae Mollissimae oven dry is peeled off, got to Chinese chestnut, temperature is 25 ℃-50 ℃, grinds to form rice-chestnut powder with kibbler then, behind the sealing bag of packing into, puts into moisture eliminator, and is subsequent use;
2, it is even to get dry rice-chestnut powder 20 Kg and the zeyssatite 10 Kg thorough mixing of above-mentioned processing, in the abstraction pool of packing into, and then adds 10 Kg zeyssatite and fills up abstraction pool, connects nitrogen device, abstraction instrument and receiving flask;
3, open nitrogen and instrument switch respectively, wait for instrument self checking, clean the abstraction instrument pipeline with cleaning solvent after self check is accomplished, set preheating temperature and begin preheating;
4, after step 3 is accomplished, pressurized liquid extraction appearance condition is set: 50 ℃ of extraction temperature, min heat-up times 4 is set, extraction pressure: 3 MPa are provided with cycle index: 1, elution volume 60 %, nitrogen blows times 60 s, begins extraction;
5, after extraction was accomplished, extraction liquid got into receiving flask, took out receiving flask, and extraction liquid is filtered; Vacuum concentration to 1/4 volume is removed albumen, and repeatable operation is not till separation surface has white suspendible; Add 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, the dry Chinese chestnut polysaccharide that gets.
Embodiment 2
1, adopt the step 1 of embodiment 1 to handle subsequent use equally;
2, it is even to get dry rice-chestnut powder 20 Kg and the zeyssatite 20 Kg thorough mixing of above-mentioned processing, in the abstraction pool of packing into, and then adds 5 Kg zeyssatite and fills up abstraction pool, connects nitrogen device, abstraction instrument and receiving flask;
3, open nitrogen and abstraction instrument switch respectively, wait for the abstraction instrument self check, clean the abstraction instrument pipeline with cleaning solvent after self check is accomplished, set preheating temperature and begin preheating;
4, behind the completing steps 3, pressurized liquid extraction appearance condition is set: 60 ℃ of extraction temperature, min heat-up times 8 is set, extraction pressure: 6 MPa are provided with cycle index: 2, elution volume 60 %, nitrogen blows times 60 s, begins extraction;
5, after extraction was accomplished, extraction liquid got into receiving flask, took out receiving flask, and extraction liquid is filtered; Vacuum concentration to 1/4 volume is removed albumen, and repeatable operation is not till separation surface has white suspendible; Add 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, the dry Chinese chestnut polysaccharide that gets.
Embodiment 3
1, adopt the step 1 of embodiment 1 to handle subsequent use equally;
2, it is even to get dry rice-chestnut powder 30 Kg and the zeyssatite 15 Kg thorough mixing of above-mentioned processing, in the abstraction pool of packing into, and then adds 5 Kg zeyssatite and fills up abstraction pool.Connect nitrogen device and abstraction instrument, abstraction pool and receiving flask are placed on the abstraction instrument specified location;
3, open nitrogen and abstraction instrument switch respectively, wait for the abstraction instrument self check, clean the abstraction instrument pipeline with cleaning solvent after self check is accomplished, set preheating temperature and begin preheating;
4, behind the completing steps 3, pressurized liquid extraction appearance condition is set: 70 ℃ of extraction temperature, min heat-up times 12 is set, extraction pressure: 9 MPa are provided with cycle index: 3, elution volume 60 %, nitrogen blows times 60 s, begins extraction;
5, after extraction was accomplished, extraction liquid got into receiving flask, took out receiving flask, and extraction liquid is filtered; Vacuum concentration to 1/4 volume is removed albumen, and repeatable operation is not till separation surface has white suspendible; Add 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, the dry Chinese chestnut polysaccharide that gets.
Embodiment 4
1, adopt the step 1 of embodiment 1 to handle subsequent use equally;
2, it is even to get dry rice-chestnut powder 20 Kg and the zeyssatite 30 Kg thorough mixing of above-mentioned processing, in the abstraction pool of packing into, and then adds 5 Kg zeyssatite and fills up abstraction pool, connects nitrogen device and abstraction instrument, and abstraction pool and receiving flask are placed on the abstraction instrument specified location;
3, open nitrogen and abstraction instrument switch respectively, wait for the abstraction instrument self check, clean the abstraction instrument pipeline with cleaning solvent after self check is accomplished, set preheating temperature and begin preheating;
4, behind the completing steps 3, pressurized liquid extraction appearance condition is set: 70 ℃ of extraction temperature, min heat-up times 4 is set, extraction pressure: 6 MPa are provided with cycle index: 3, elution volume 60 %, nitrogen blows times 60 s, begins extraction;
5, after extraction was accomplished, extraction liquid got into receiving flask, took out receiving flask, and extraction liquid is filtered; Vacuum concentration to 1/4 volume is removed albumen, and repeatable operation is not till separation surface has white suspendible; Add 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, the dry Chinese chestnut polysaccharide that gets.
The foregoing description only is used for the reference as technical scheme of the present invention, not as the single restricted condition of technical scheme itself.
Claims (3)
1. a technology of utilizing the pressurized liquid extraction method to extract the Chinese chestnut polysaccharide is characterized in that, comprising:
(1) Chinese chestnut is peeled off, get the kernel of Semen Castaneae Mollissimae oven dry, temperature is 25 ℃-50 ℃, grinds to form rice-chestnut powder with kibbler then, behind the sealing bag of packing into, puts into moisture eliminator, and is subsequent use;
(2) it is even to get the dry rice-chestnut powder and the zeyssatite thorough mixing of above-mentioned processing, in the abstraction pool of packing into, and then adds 1 ~ 20 kg zeyssatite and fills up abstraction pool, connects nitrogen device, abstraction instrument and receiving flask;
(3) open nitrogen and abstraction instrument switch respectively, the abstraction instrument self check is cleaned the abstraction instrument pipeline with cleaning solvent, sets preheating temperature and begins preheating;
(4) after step 3 was accomplished, pressurized liquid extraction appearance condition is set: 50 ℃-70 ℃ of extraction temperature were provided with min-20 min heat-up times 4; Extraction pressure: 3 MPa-15 MPa are provided with cycle index: 1-5 time, and elution volume 40 %-60 %; Nitrogen blows time 40-100 s, begins extraction;
(5) after extraction was accomplished, extraction liquid got into receiving flask, and extraction liquid is filtered, and vacuum concentration to 1/4 volume is removed albumen, and repeatable operation till separation surface does not have white suspendible, adds 75 %-100 % ethanol, alcohol precipitation 5 ~ 12 h, dry Chinese chestnut polysaccharide.
2. the technology of extraction Chinese chestnut polysaccharide according to claim 1 is characterized in that rice-chestnut powder in the said step (2) and zeyssatite are pressed 20 Kg: the mixed of 10 ~ 30 Kg.
3. the technology of extraction Chinese chestnut polysaccharide according to claim 1 is characterized in that described cleaning solvent and extraction solvent are pure water.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142721A (en) * | 2013-03-15 | 2013-06-12 | 河北科技师范学院 | Method for extracting triterpenoid from anli pear |
| CN104592412A (en) * | 2015-02-28 | 2015-05-06 | 周可幸 | Method for extracting chestnut polysaccharide |
| CN104861085A (en) * | 2015-06-16 | 2015-08-26 | 河北大学 | Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs |
| CN105754001A (en) * | 2016-04-26 | 2016-07-13 | 吉林化工学院 | Method for extracting polysaccharides in polygonatum odoratum through solvent pressurizing method |
| CN107880147A (en) * | 2017-12-26 | 2018-04-06 | 河北科技师范学院 | Chinese chestnut active anticancer polysaccharide and its isolation and purification method |
| CN107982276A (en) * | 2017-12-26 | 2018-05-04 | 河北科技师范学院 | Purposes of the chestnut polysaccharide in the medicine for suppressing growth of cancer cells is prepared |
| CN109134690A (en) * | 2018-11-12 | 2019-01-04 | 运城学院 | A kind of ultrasonic wave auxiliary Polysaccharide in Pleurotus eryngii extraction process |
| CN114522926A (en) * | 2022-02-18 | 2022-05-24 | 茶陵晶辉电子实业有限公司 | Integral display screen cleaning equipment and cleaning method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103142721A (en) * | 2013-03-15 | 2013-06-12 | 河北科技师范学院 | Method for extracting triterpenoid from anli pear |
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| CN104861085A (en) * | 2015-06-16 | 2015-08-26 | 河北大学 | Chestnut seed alpha-1,6-glucan, preparation method for alpha-1,6-glucan, and application of alpha-1,6-glucan to anticancer drugs |
| CN105754001A (en) * | 2016-04-26 | 2016-07-13 | 吉林化工学院 | Method for extracting polysaccharides in polygonatum odoratum through solvent pressurizing method |
| CN107880147A (en) * | 2017-12-26 | 2018-04-06 | 河北科技师范学院 | Chinese chestnut active anticancer polysaccharide and its isolation and purification method |
| CN107982276A (en) * | 2017-12-26 | 2018-05-04 | 河北科技师范学院 | Purposes of the chestnut polysaccharide in the medicine for suppressing growth of cancer cells is prepared |
| CN109134690A (en) * | 2018-11-12 | 2019-01-04 | 运城学院 | A kind of ultrasonic wave auxiliary Polysaccharide in Pleurotus eryngii extraction process |
| CN114522926A (en) * | 2022-02-18 | 2022-05-24 | 茶陵晶辉电子实业有限公司 | Integral display screen cleaning equipment and cleaning method |
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Application publication date: 20121003 |