CN1181208C - Production method of oligose and beverage containing same - Google Patents
Production method of oligose and beverage containing same Download PDFInfo
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- CN1181208C CN1181208C CNB031208916A CN03120891A CN1181208C CN 1181208 C CN1181208 C CN 1181208C CN B031208916 A CNB031208916 A CN B031208916A CN 03120891 A CN03120891 A CN 03120891A CN 1181208 C CN1181208 C CN 1181208C
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- wheat bran
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Abstract
The present invention relates to a method using wheat bran for producing oligosaccharides and a beverage containing the oligosaccharides generated by the method. The method comprises the steps of drying and pulverizing the wheat bran, adding deionized water into the wheat barn for immersion, homogenizing the wheat bran, adding the complex phosphoesterasum formulation composed of phytase, cellulose microcrystalline, proteinase and oligosaccharides enzyme for the enzymolysis of the wheat bran, adjusting the pH value, decomposing the enzyme of the wheat bran, obtaining the clear liquid containing oligosaccharides after centrifugation and filtration, and concentrating the clear liquid in vacuum in order to obtain a concentrated mixture containing soluble oligosaccharides solid contents. In the complex phosphoesterasum formulation, the phytase dosage is 10 to 30 U/g of wheat bran, the cellulose microcrystalline dosage is 10 to 20 U/g of wheat bran, the proteinase dosage is 30 to 40 U/g of wheat bran and the oligosaccharides enzyme dosage is 20 to 30 U/g of wheat bran. The complex phosphoesterasum formulation can also comprise amylase of which the dosage is 10 to 20 U/g of wheat bran. Ethanol, sodium hydroxide and trichloroacetic acid can also be used for removing soluble protein and peptide in order to purify the oligosaccharides. The method has the advantages of short used time, high efficiency and low cost and is suitable for industrial application.
Description
Technical field
The present invention relates to the production method of oligose and contain the beverage of the oligose of method production thus, particularly relate to by Testa Tritici and produce the method for oligose and contain the beverage of the oligose of method production thus.
Background technology
The annual wheat processing byproduct of China wheat bran amount is up to more than 2,000 ten thousand tons at present, and the utilization of wheat bran mainly is directly as animal and fowl fodder, fermention medium etc.Therefore, cheap.That seeks wheat bran effectively utilizes approach, improves the wheat bran utility value and has crucial economic benefit and social benefit.The main component of wheat bran is a carbohydrate, accounts for about 60%, and the development functionality oligose is one of effective way of wheat bran increment and comprehensive utilization from the wheat bran carbohydrate.
The peculiar nutrition of Testa Tritici oligose can be widely used in medicine industry and foodstuffs industry.Since the quickening of modern society people rhythm of life, the variation of dietary structure, and new problem has appearred in people's health.Clinical investigation finds that changing into serves as that main new fatal disease is composed with obesity, hyperlipidemia, diabetes, cancer etc. now in China recent years resident's the deadly spectrum of disease communicable disease by the 50-60 age.These change and the variation of resident's diet formula has significant effects, and wherein important aspect is exactly the serious decline that food fibre is taken in.Replenish the Testa Tritici oligose to improving edibility food fibre intake, can avoid pulvis to be difficult to dissolving with the form of beverage, the problem that is difficult to absorb is rationally allocated according to the human body requirement, has both obtained the enjoyment on the satisfied taste of getting back of nutrition.
The Testa Tritici oligose has following biological function:
1 oligose main chain is polymerized by 4 hexoses, in the aqueous solution, can be split into helicoidal structure under the certain condition, so its water-holding power is stronger.In protein drinks, add this oligose and can effectively improve protein drinks stability, play thickening effectiveness.
2 have good bifidus bacillus cultivation effect, and it is not utilized by many microorganisms such as harmful bacterium in animal intestinal, and are only utilized by some probioticss of genus bifidobacterium, can be used as the bifidobacterium growth factor and are applied to food;
3 have the low heat value performance, belong to indigestibility sugar, do not utilized by the product acids in the oral cavity and other microorganism, thereby the resolvent of heavy collection reduces on the tooth, show anti-dental caries function, because its low heat value performance and to the hormesis of diabetes body insulin secretion can be used it for and produce the oligose product as patient's ideal food such as diabetes, obesity, hyperlipidemia;
4 oligose also have surfactivity, and toxic substance and pathogenic bacteria in the adsorbable enteron aisle can be improved body resistance against diseases, activating immune system.
At present, the technology of preparing of utilizing Testa Tritici to prepare oligose is mainly carried out enzymolysis by adding amylase and proteolytic enzyme in the Testa Tritici of sizing mixing, and then adds oligosaccharidase decolouring after filtration again, concentrates after the ion-exchange, makes finished product after the drying.
Above-mentioned technical matters laboratory study is feasible, but the long processing time of actual production, industrialized operation sequence is loaded down with trivial details.Although can increase the yield of oligose behind the enzymolysis, remaining phytic acid, starch, monose, disaccharide, phenolic acid, protein, flavonoid, lignan are difficult to effectively oligose and they be separated through filtration and decoloration process; The greatest weakness of ion-exchange is that efficient is low, and the time is long, so the industrialization technology of wheat bran oligose still is in the laboratory study stage so far.Hindered the industrial applications of wheat bran oligose thus.
Summary of the invention
For overcoming the defective of above-mentioned prior art, the purpose of this invention is to provide a kind of method of producing oligose by Testa Tritici, this method is suitable for industrial applications, the efficient height, cost is low, and the required time is short.
And then another object of the present invention provides a kind of beverage that contains the oligose of being produced by the inventive method.Because the These characteristics of the inventive method, make this beverage can simplify the operation operation, suitability for industrialized production low-costly and in high volume, thereby a kind of low heat energy beverage that is suitable for obesity, diabetes, hyperlipemic patients is provided with oligose advantage.
For achieving the above object, the method by Testa Tritici production oligose of the present invention may further comprise the steps:
With the Testa Tritici crushed after being dried;
Adding deionized water soaks;
Carry out homogenization treatment;
Adjust pH value, add the compound enzymic preparation that includes phytase, cellulase, proteolytic enzyme and oligosaccharidase and carry out enzymolysis;
Killing enzyme handles;
Centrifugal and filter after obtain containing the clear liquid of oligose;
Above-mentioned clear liquid is carried out vacuum concentration, to obtain containing the enriched mixture of soluble oligosaccharide solid substance.
According to an aspect of the present invention, the compound enzymic preparation that uses in the described enzymolysis also can comprise amylase, wherein the phytase consumption is the 10-30U/g wheat bran, the cellulase consumption is the 10-20U/g wheat bran, the proteolytic enzyme consumption is the 30-40U/g wheat bran, the oligosaccharidase consumption is the 20-30U/g wheat bran, and the amylase consumption is the 10-20U/g wheat bran;
According to a further aspect of the invention, further comprising the steps of:
In described enriched mixture, add ethanol, go out the oligose throw out through centrifugation;
In the oligose throw out, add pure water and utilize sodium hydroxide to adjust the pH value of extracting solution, and add trichoroacetic acid(TCA), to remove the protein in the oligose;
Centrifugally dispel soluble protein and peptide and isolate the clear liquid that is dissolved with oligose, realize the purifying of oligose thus.
According to a further aspect of the invention, also comprise and dispel soluble protein and peptide and isolate the clear liquid that is dissolved with oligose and carry out spissated step centrifugal.
Characteristics of the present invention are, are that the hydrolysis of phytase, amylase, proteolytic enzyme, cellulase and oligosaccharidase is handled in same operation in the enzymolysis processing behind the homogeneous, have simplified manufacturing procedure, improve the yield and the extraction efficiency of oligose; Supernatant liquor after the filtration utilizes ethanol sedimentation to dispel rapidly with impurity such as the miscible solubility monose of oligose, disaccharide, soluble peptide, amino acid after concentrating; Add the ethanol sedimentation oligose after the supernatant concentration, ethanol in the concentrating under reduced pressure process through industrialized recovery rectifying reused, reduce production costs greatly, solved oligose is extracted in industrialization from wheat bran technical matters; After utilizing sodium hydroxide to adjust extracting solution PH6.2, add trichoroacetic acid(TCA), can dispel soluble protein and peptide, do not have the residue problem of nuisance.Extracting solution is concentrated, need be configured as functional drinks, avoided dry back oligose indissoluble problem, drink conveniently by human body.
Embodiment
According to a preferred embodiment of the present invention, the technological process of production of Testa Tritici soluble oligosaccharide is as follows: wheat bran → pulverizing → homogeneous → size mixing → compound enzyme one-step enzymolysis → separating and filtering → ethanol sedimentation → add water redissolution → trichoroacetic acid(TCA) remove albumen → centrifugal → stillness of night → concentrate standbyly, finally obtain wheat bran oligose concentrated solution.
State in realization in the specific embodiment of production technique, may further comprise the steps:
With the Testa Tritici drying, pulverize after through 60 mesh sieve powder;
Add 2-3 times of deionized water in the Testa Tritici after pulverizing;
Heating is 20-30 minute under 50 ℃ of conditions;
Homogeneous under 25-35Mpa pressure;
Under 45-55 ℃ of condition, add the compound enzymic preparation that includes phytase, cellulase, proteolytic enzyme, amylase and oligosaccharidase, wherein the phytase consumption is the 10-30U/g wheat bran, the cellulase consumption is the 10-20U/g wheat bran, the proteolytic enzyme consumption is 30-40U/g, and the amylase consumption is the 10-20U/g wheat bran, and the oligosaccharidase consumption is the 20-30U/g wheat bran, and utilize 1% sodium hydroxide solution to adjust to PH5.2, to wheat bran enzymolysis 90 minutes;
Heat 10-15 minute enzyme extremely down at 90 ℃;
Cooling is gone into vacuum concentration pot through the filtering supernatant liquor of 40 keevil frames after 5000 rev/mins after centrifugal 10 minutes, is concentrated into soluble solid 〉=10%;
Add 95% ethanol to 75-80%, be settled out oligose in centrifugal 10 minutes through 5000 rev/mins;
Adding 3-4 times of pure water and utilize 1% sodium hydroxide to adjust extracting solution PH in the oligose throw out is 6.2, and adds 30-40% trichoroacetic acid(TCA) at 20: 1 by the volume ratio with mother liquor, reacts 10 minutes, to remove the protein in the oligose;
Dispeled soluble protein and peptide and isolated the supernatant liquor that is dissolved with oligose in centrifugal 10 minutes for 5000 rev/mins; With this supernatant concentration to soluble solid 〉=10%.
The technological process of production according to the peanut beverage that contains the oligose of being produced by the inventive method of the present invention is as follows: peanut toasts → goes scarlet → defibrination → enzymolysis → filtration → adding oligose allotment → homogeneous → degassing → canned → seal → sterilization.Main technologic parameters wherein is: peanut is stirred baking 20 minutes under 90 ℃ of conditions, make the Semen arachidis hypogaeae friction through sander, scarlet is come off, after wind, scarlet is removed, the pure water of Semen arachidis hypogaeae and 2 times is added paste mill grinding, under 55 ℃ of conditions, added the 16U/g protease hydrolyzed 50 minutes, after 40 keevil frame filters filter, filtrate is added 3% oligosaccharide syrup, pure water, adding concentration is 2/0,000 xanthan gum and carboxymethyl cellulose (wherein xanthan gum accounts for 1/2), finally making soluble solid by the adjustment amount of water is 5-10%, the balanced protein sugar that adds, sorbyl alcohol, Xylitol each 0.1/ ten thousand, utilize 10% yellow soda ash to adjust pH value and be 6.8-7.0, pass through homogeneous under the pressure of 30MPA then, under the vacuum condition of normal temperature 75mm mercury column, took off vapour 20 minutes, after canned, sterilization is 30 minutes under 121 ℃ of conditions, and after the spray cooling, warehouse-in is treated pin.Other processing parameters in this production technique and technique means all are known, its explanation of Therefore, omited.。
The index of the drink product quality that goes out by above-mentioned explained hereafter is: oligosaccharide contg 〉=1%; Total protein content 〉=0.8%; Total dissoluble solid content≤10%; Protein sugar, sorbyl alcohol, Xylitol are an amount of.All other indexs of health reach the GB requirement.
Except that other indicated, the concentration of mentioning among the application was weight concentration.
The characteristics of this beverage of the present invention are: this product utilizes zymolysis technique to improve content of soluble protein in the Semen arachidis hypogaeae protein milky-drinks first, proteinic digestibility and nutritive value have been improved, improve utilization ratio of raw materials and quality product, reduced the precipitation of product; Composite in the technology have the bifidus bacillus cultivation effect and improve the wheat oligose of immunization; Utilize adding protein sugar, sorbyl alcohol, Xylitol and substitute the conventional sucrose that uses, so the low heat energy of product, is hyperlipidemia, hyperglycemia population and the desirable drink that is used to lose weight.Especially oligose is extracted in the Testa Tritici industrialization that will not have mass-producing to utilize at present, and the oligose product is used in the low heat energy of exploitation increases the drink product of bifid effect, has novelty and industrial applications and is worth.
Though done clear and definite and specific description at realization the preferred embodiments of the present invention above, but those of ordinary skill in the art can make other scheme of the present invention of implementing by the various processing parameters that proposed above changing fully within design of the present invention and scope.These change the protection domain that all belongs to the application.
Claims (6)
1. method of producing oligose by Testa Tritici, it may further comprise the steps:
With the Testa Tritici crushed after being dried;
Adding deionized water soaks;
Carry out homogenization treatment;
Adjust pH value;
Add the compound enzymic preparation that includes phytase, cellulase, proteolytic enzyme and oligosaccharidase and carry out enzymolysis;
Killing enzyme handles;
Centrifugal and filter after obtain containing the clear liquid of oligose;
Above-mentioned clear liquid is carried out vacuum concentration, to obtain containing the enriched mixture of soluble oligosaccharide solid substance.
2. method according to claim 1, it is characterized in that: the compound enzymic preparation that uses in the described enzymolysis also comprises amylase, wherein the phytase consumption is the 10-30U/g wheat bran, the cellulase consumption is the 10-20U/g wheat bran, the proteolytic enzyme consumption is the 30-40U/g wheat bran, the oligosaccharidase consumption is the 20-30U/g wheat bran, and the amylase consumption is the 10-20U/g wheat bran.
3. method according to claim 2 is characterized in that further comprising the steps of:
In described enriched mixture, add ethanol, go out the oligose throw out through centrifugation;
In the oligose throw out, add pure water and utilize sodium hydroxide to adjust the pH value of extracting solution, and add trichoroacetic acid(TCA), to remove the protein in the oligose;
Centrifugally dispel soluble protein and peptide and isolate the clear liquid that is dissolved with oligose, realize the purifying of oligose thus.
4. method according to claim 3 is characterized in that also comprising and dispels soluble protein and peptide and isolate the clear liquid that is dissolved with oligose and carry out spissated step centrifugal.
5. method according to claim 4 is characterized in that: the alcoholic acid add-on is that 95% ethanol is to 75-80%; The add-on of pure water is the sedimentary 3-4 of oligose times; Concentration sodium hydroxide is 1%, and pH value is adjusted into 6.2; The concentration of trichoroacetic acid(TCA) is 30-40%, and add-on is 20: 1 for the volume ratio with mother liquor, and reacts 10 minutes; To centrifugal dispel soluble protein and peptide and isolate the clear liquid that is dissolved with oligose be concentrated into soluble solid 〉=10%.
6. according to each described method among the claim 1-5, it is characterized in that the processing parameter of described each step is as follows: in the step with the Testa Tritici crushed after being dried, the wheat bran of pulverizing is through 60 mesh sieve powder; In soaking step, the add-on of deionized water is 2-3 a times of Testa Tritici, and heats 20-30 minute under 50 ℃ of conditions; The pressure of homogenization treatment is 25-35MPa; The temperature that enzymolysis carries out is 45-55 ℃, and utilizes 1% sodium hydroxide solution to adjust to PH5.2, to time of wheat bran enzymolysis be 90 minutes; The temperature of killing the enzyme processing is 90 ℃, and the time is 10-15 minute; In centrifugal and filtration step, filter with 40 keevil frames; Vacuum concentration proceeds to soluble solid 〉=10%; The centrifugal centrifugal speed that is in the described step is 5000 rev/mins, and the time is 10 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031208916A CN1181208C (en) | 2003-03-25 | 2003-03-25 | Production method of oligose and beverage containing same |
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| CNB031208916A CN1181208C (en) | 2003-03-25 | 2003-03-25 | Production method of oligose and beverage containing same |
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| CN1181208C true CN1181208C (en) | 2004-12-22 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100383239C (en) * | 2004-07-21 | 2008-04-23 | 华南农业大学 | Process for extracting oxalate oxidase from bran of wheat |
| CN101455397B (en) * | 2009-01-07 | 2012-01-11 | 吉林农业大学 | Animals and plants polysaccharide oligomerization method |
| CN102174601A (en) * | 2011-01-05 | 2011-09-07 | 天津工业大学 | Method for producing raw material for fermentation, edible oil and protein by using highland barley bran |
| CN102334628B (en) * | 2011-06-29 | 2012-11-07 | 江南大学 | Preparation method for prebiotic preparation |
| CN102613364B (en) * | 2012-04-24 | 2013-06-05 | 四川农业大学 | Preparation method of wheat bran fragrant tea |
| CN104856174A (en) * | 2015-04-28 | 2015-08-26 | 天津科技大学 | Oat bran polysaccharide composite weight-reducing beverage and applications thereof |
| CN107254457B (en) * | 2017-08-15 | 2020-06-23 | 青岛澳蓝明东生物科技有限公司 | Special complex enzyme preparation for improving conversion rate of xylopentaose and application thereof |
| CN108719867A (en) * | 2018-04-23 | 2018-11-02 | 西北农林科技大学 | A kind of big jujube products and preparation method thereof rich in oligosaccharide |
| CN109170461A (en) * | 2018-09-03 | 2019-01-11 | 溧阳市迪贝乐生物科技有限公司 | A kind of oligofructose solid beverage and preparation method thereof |
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