CN107254457B - Special complex enzyme preparation for improving conversion rate of xylopentaose and application thereof - Google Patents
Special complex enzyme preparation for improving conversion rate of xylopentaose and application thereof Download PDFInfo
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Abstract
The invention discloses a special compound enzyme preparation for improving the conversion rate of xylopentaose, which comprises xylanase, phytase, mannase and sodium carbonate. The complex enzyme preparation can prepare the xylo-oligosaccharide solution taking xylose as a main component by taking the squeezed liquid produced by viscose as a raw material, greatly improves the conversion rate of the xylo-oligosaccharide through the synergistic effect of sodium carbonate and xylanase, phytase and mannase in the complex enzyme preparation, and obtains good effect in the culture of animal bifidobacterium.
Description
Technical Field
The invention belongs to the technical field of enzyme preparation application, and particularly relates to a special complex enzyme preparation for improving the conversion rate of xylopentaose and application thereof.
Background
Xylo-oligosaccharide is a functional oligosaccharide, is a mixture formed by connecting 2-7D-xyloses by β -1, 4-xyloside bonds, and partially contains arabinouronic acid and glucuronic acid side chains, the xylo-oligosaccharide is active sugar which can promote the growth of beneficial bacteria in vivo in the existing functional oligosaccharide, and has physiological functions of preventing dental caries, promoting calcium absorption, reducing blood pressure and serum cholesterol, inhibiting intestinal pathogenic bacteria, preventing constipation and diarrhea and the like.
Currently, the raw material sources for preparing xylo-oligosaccharide are corncob, bagasse, reed and wood, and most of the patents relate to corncob. The patent reports of the complex enzyme preparation for preparing xylo-oligosaccharide from wood are less. The invention patent CN1266633A discloses a production process for preparing functional xylo-oligosaccharide by an enzyme method, which takes corncobs, corn stalks, straws and the like as raw materials, and prepares the xylo-oligosaccharide by screening, drying, crushing, soaking with water, dehydrating, leaching with alkali, neutralizing with acid, and finally adding xylanase for hydrolysis.
In recent years, with the continuous development and deepening of micro-ecological theory research, a means for adjusting the micro-ecological balance in a human body as a disease prevention, treatment and health care effect by applying a micro-ecological preparation forms a hot tide worldwide. At present, hundreds of micro-ecological preparations are developed and applied at home and abroad. The active bifidobacterium preparation is used as a majority, the bifidobacterium is an important beneficial microorganism in human intestinal flora, has the biological barrier effect, has the effects of controlling intracellular toxemia, reducing autoxidation and regulating the intestine, can restore the normal level of the intestinal flora, and has strong antagonistic effect on pathogenic bacteria.
The study of bifidobacteria is largely divided into two areas: on one hand, the number of bifidobacteria in a human body is increased by directly ingesting live bifidobacteria, but the bacteria belong to obligate anaerobes, are very sensitive to oxygen, have poor resistance to low pH, are difficult to maintain activity, and most products on the market have low live bacteria amount and are completely dead after being stored for less than one month. On the other hand, the proliferation of bifidobacteria in vivo is promoted by the intake of carbon source. Although glucose has a good effect on the proliferation of beneficial bacterial flora in the human body such as bifidobacterium, the bifidobacterium flora is mainly localized in the large intestine of the human body, and the glucose converted from starch food is difficult to reach the large intestine after the starch food is digested and absorbed by digestive organs such as the oral cavity, the stomach and the small intestine of the human body. In contrast, xylo-oligosaccharide is hardly digested and absorbed by human body and hardly utilized by harmful bacteria in human body, so that xylo-oligosaccharide can be used as bifidus factor to directly proliferate bifidobacterium in human body. A Zhang Xiaoping paper shows that the xylooligosaccharide with the polymerization degree being larger than or equal to 4 can reduce the accumulation of xylose and xylobiose, improve the utilization of bifidobacterium adolescentis on the saccharides, particularly the utilization of xylotetraose and xylopentaose, and simultaneously obtain higher thallus concentration, thereby showing that the effect of proliferating the bifidobacterium adolescentis by the xylooligosaccharide with higher polymerization degree is better than that of the xylooligosaccharide with lower polymerization degree.
The invention patent CN 106754828A of the applicant of the present application, which is applied before, discloses a compound enzyme for preparing xylo-oligosaccharide from eucalyptus, which comprises xylanase and cellulase; the complex enzyme also comprises mannase. The complex enzyme can be widely applied to the production of xylo-oligosaccharide, the efficiency of converting eucalyptus hemicellulose into xylo-oligosaccharide can be effectively improved, the degradation effect of xylanase on hemicellulose can be obviously promoted by cellulase and mannase in the complex enzyme, the total conversion rate of xylobiose, xylotriose and xylotetraose can be improved by 12.5% under the synergistic action of the three enzymes, the conversion rate of xylotetraose is also improved by 50%, but the conversion rate of the xylopentaose prepared by using the complex enzyme preparation is extremely low.
Therefore, most of the reports in the prior literature are methods and enzyme preparations for producing xylooligosaccharide mainly containing xylobiose and xylotriose, and the production of xylopentaose is only focused on, so the invention aims to develop xylooligosaccharide with the polymerization degree being not less than 4, particularly to increase the content of the xylopentaose in the xylooligosaccharide for improving the yield of bifidobacterium.
Disclosure of Invention
The invention provides a special complex enzyme preparation for improving the conversion rate of xylopentaose and application thereof to solve the technical problems in the prior art, and the special complex enzyme preparation is used for improving xylooligosaccharide with the polymerization degree being larger than or equal to 4, particularly increasing the content of xylopentaose in the xylooligosaccharide and improving the yield of bifidobacterium.
A special complex enzyme preparation for improving the conversion rate of the xylopentaose comprises xylanase, phytase and mannase.
Further, the compound enzyme preparation also comprises sodium carbonate.
Preferably, the weight ratio of xylanase, phytase, mannase and sodium carbonate in the complex enzyme preparation is 2: 0.2-0.5: 0.5-0.2: 0.5-4.
Preferably, the weight ratio of xylanase, phytase, mannase and sodium carbonate in the complex enzyme preparation is 2: 0.2-0.5: 0.5-0.2: 1.5-3.5.
Preferably, the weight ratio of xylanase, phytase, mannase and sodium carbonate in the complex enzyme preparation is 2: 0.2-0.5: 0.5-0.2: 2-3.
Preferably, the weight ratio of xylanase, phytase, mannase and sodium carbonate in the complex enzyme preparation is 2: 0.2-0.5: 0.5-0.2: 2.5.
further, the complex enzyme preparation disclosed by the invention is applied to improving the conversion rate of the xylopentaose.
Further, the invention also provides a method for improving the conversion rate of the xylopentaose by using the complex enzyme preparation, which comprises the following steps:
1) performing membrane dealkalization and concentration on squeezed liquid produced by viscose fibers to obtain a hemicellulose solution, mixing dilute sulfuric acid with the concentration of 12% and the hemicellulose solution in equal volume, treating at the high temperature of 100 ℃ for 90min, and adjusting the pH value to be neutral by using calcium carbonate;
2) adjusting the pH value of the hemicellulose solution to 5.5 by using concentrated sulfuric acid, adding the complex enzyme preparation according to the proportion of 10-12g/Kg of xylan, and acting for 6-12h at 50-60 ℃;
3) after the enzymolysis reaction is finished, heating to more than 80 ℃, preserving the temperature for 15min, and inactivating the complex enzyme;
4) and (3) carrying out solid-liquid separation on the solution after enzymolysis through a plate frame to obtain a clear solution, namely the xylo-oligosaccharide solution.
Further, the invention also provides a method for improving the conversion rate of the xylopentaose by using the complex enzyme preparation, which comprises the following steps:
1) performing membrane dealkalization and concentration on squeezed liquid produced by viscose fibers to obtain a hemicellulose solution, mixing dilute sulfuric acid with the concentration of 12% and the hemicellulose solution in equal volume, treating at the high temperature of 100 ℃ for 90min, and adjusting the pH value to be neutral by using calcium carbonate;
2) adjusting the pH value of the hemicellulose solution to 5.5 by using concentrated sulfuric acid, adding the complex enzyme preparation according to the proportion of 10.8g/Kg of xylan, and acting for 6-12h at 50-60 ℃;
3) after the enzymolysis reaction is finished, heating to more than 80 ℃, preserving the temperature for 15min, and inactivating the complex enzyme;
4) and (3) carrying out solid-liquid separation on the solution after enzymolysis through a plate frame to obtain a clear solution, namely the xylo-oligosaccharide solution.
Preferably, in the complex enzyme preparation, the enzyme activities of xylanase, phytase and mannase are 10000U/g, 5000U/g and 2000U/g respectively.
Further, the application of the xylo-oligosaccharide solution prepared by the method of the invention in bifidobacterium culture.
Preferably, the Bifidobacterium is Bifidobacterium animalis (Bifidobacterium animalis).
Compared with the prior art, the method has the following advantages:
(1) in the xylo-oligosaccharide solution prepared by treating the viscose squeezed liquid with the complex enzyme preparation, the content of the xylo-oligosaccharide is remarkably improved, and the conversion rate of the xylo-oligosaccharide is up to 42%.
(2) In the complex enzyme preparation, the influence of the addition amount of sodium carbonate on the conversion rate of the xylopentaose is very obvious. With the increase of the addition amount of the sodium carbonate, the conversion rate of the xylo-oligosaccharide in the xylo-oligosaccharide solution is slowly improved, but when the addition amount of the sodium carbonate in the complex enzyme preparation reaches 2.0kg, the conversion rate of the xylo-oligosaccharide is greatly improved and exceeds 40%, and when the addition amount of the sodium carbonate in the complex enzyme preparation exceeds 3.0kg, the conversion rate of the xylo-oligosaccharide begins to rapidly decrease. Therefore, the sodium carbonate can generate a synergistic effect with the xylanase, the phytase and the mannase in the compound enzyme to promote the conversion of the xylopentaose, and particularly when the addition amount of the sodium carbonate in the compound enzyme preparation is 2.0-3.0kg, the synergistic effect is strongest, the conversion rate of the xylopentaose can be greatly improved, and unexpected technical effects are achieved.
(3) The xylo-oligosaccharide solution prepared by the invention is used as a carbon source to culture the animal bifidobacterium, so that the thallus concentration of the animal bifidobacterium can be obviously increased, and the thallus concentration is respectively increased by 52 percent and 50.5 percent at 24 hours and 48 hours, thereby showing that the increase of the content of xylo-oligosaccharide in the xylo-oligosaccharide can effectively promote the proliferation of the animal bifidobacterium, shorten the thallus culture period, reduce the production cost, increase the economic benefit and have obvious effect.
In conclusion, the complex enzyme preparation can prepare the xylo-oligosaccharide solution taking xylose as the main component by taking the squeezed liquid produced by viscose as the raw material, greatly improves the conversion rate of the xylo-oligosaccharide through the synergistic effect of the sodium carbonate and the xylanase, the phytase and the mannase in the complex enzyme preparation, and obtains good effect in the culture of the animal bifidobacterium.
Detailed Description
In the viscose fiber production process, raw material paper pulp is mainly dissolved by an alkaline method, specifically, hemicellulose in the paper pulp is dissolved by using a sodium hydroxide solution. After the alkali is dissolved, the raw material is squeezed to obtain a solution, namely the viscose squeezing solution in the embodiment of the invention, and the main components of the solution are hemicellulose and sodium hydroxide.
In the embodiment of the invention, the ion chromatography is adopted to measure the contents of glucose, xylose, xylobiose, xylotriose, xylotetraose and xylopentaose, and the measuring method specifically comprises the following steps:
principle 1
When a sample enters a stationary phase (chromatographic column) along with a mobile phase, components dissolved in the mobile phase pass through the stationary phase, due to different sizes and strengths of actions (adsorption, distribution, ion attraction, exclusion and affinity) with the stationary phase, the components are retained in the stationary phase for different time periods, so that the components flow out of the stationary phase in sequence, the components retained in the stationary phase for different retention time periods enter an electrochemical detector, an oxidation-reduction reaction is carried out, signals with different strengths are generated, a chromatogram is formed by the signals, and the signal value and the concentration are in a linear relationship within a certain range.
The electrochemical detection working principle is as follows: the saccharide belongs to weak acid, and can be partially or completely formed into anion in strong alkaline solution, and can be separated by utilizing different retentivity on an anion chromatographic column, under the condition of strong alkaline, the electrochemical active groups of hydroxyl group and the like in the saccharide structure can be oxidized on the surface of a gold electrode under a proper potential to cause the change of current, and the magnitude of the current generates different chromatographic peaks.
2 measurement procedure
2.1 Standard Curve
Taking glucose, xylose, xylobiose, xylotriose, xylotetraose and xylopentaose standard solutions, diluting by 10 times, and then diluting according to the following table to obtain standard solutions for experiments:
| mixing Standard solution (ml) | Deionized water (ml) | Total volume (ml) |
| 0.1 | 0.9 | 1 |
| 0.3 | 0.7 | 1 |
| 0.5 | 0.5 | 1 |
| 0.7 | 0.3 | 1 |
| 1.0 | 0 | 1 |
2.2 Experimental conditions
Ion chromatography: IC-5000
A detector: electrochemical detector
Flow rate: 0.7ml/min
Column temperature: 30 deg.C
A chromatographic column: thermo Fisher PA10
Protection of the column: thermo Fisher GA10
pH electrode: agcl
Potential: standard quad
Sample introduction amount: 25ul
Mobile phase: 0.25mol/L sodium hydroxide: water 40:60
Operating time: 45min
And (3) peak appearance sequence: glucose, xylose, xylobiose, xylotriose, xylotetraose and xylopentaose
3 results and calculations of the experiments
3.1 preparation of Standard Curve
Taking the concentration of glucose, xylose, xylobiose, xylotriose, xylotetraose and xylopentaose as the abscissa and the response value of the chromatographic peak area as the ordinate, a linear regression equation is listed: y ═ ac + b;
wherein Y is the peak area of the sugar, a and b are standard equation coefficients, and c is the concentration of the corresponding sugar.
3.2 sample content calculation
The formula: p ═ C × n;
wherein P is the content of the certain sugar in the sample, C is the concentration of the corresponding sugar calculated by the standard curve, and n is the dilution factor.
The xylanase, phytase and mannase described in this example can be purchased from Weifang Kangdian Biotechnology Limited, and the enzyme activities are respectively xylanase 10000U/g, phytase 5000U/g and mannase 2000U/g; the sodium carbonate is available from the national pharmaceutical group.
Example 1
The special composite enzyme for producing the xylopentaose comprises 2Kg of xylanase, 0.2Kg of phytase, 0.2Kg of mannase and 2.0Kg of sodium carbonate.
The special complex enzyme is obtained by weighing the components according to the proportion and uniformly mixing the components.
Example 2
The special composite enzyme for producing the xylopentaose comprises 2Kg of xylanase, 0.2Kg of phytase, 0.5Kg of mannase and 2.3Kg of sodium carbonate.
The preparation method is the same as example 1.
Example 3
The special composite enzyme for producing the xylopentaose comprises 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 3.0Kg of sodium carbonate.
The preparation method is the same as example 1.
Example 4 application of Complex enzyme in production of Wood Pentagon from viscose fiber pressed liquor
1. Carrying out membrane dealkalization and concentration on a squeezed liquid produced by viscose fibers to obtain a low-alkali high-concentration hemicellulose solution;
mixing 20ml of hemicellulose solution with 20ml of 12% dilute sulfuric acid, treating at a high temperature of 100 ℃ for 90min, adjusting the pH to be neutral by using calcium carbonate, and measuring the total content of xylan in the hemicellulose solution by using ion liquid chromatography, wherein the result shows that the xylan content in the obtained hemicellulose solution is 50 g/L;
2. adjusting the pH value of the hemicellulose solution to 5.5 by using concentrated sulfuric acid;
3. adding the complex enzyme according to the proportion of 10-12g/Kg of xylan, and reacting at 50-60 ℃ for 6-12 h;
4. after the enzymolysis reaction is finished, heating to more than 80 ℃, preserving the temperature for 15min, and inactivating the complex enzyme;
5. and (3) carrying out solid-liquid separation on the solution after enzymolysis through a plate frame to obtain a clear solution, namely the xylo-oligosaccharide solution.
The content of the xylo-oligosaccharide in the xylo-oligosaccharide solution obtained in the above way is measured by using ion liquid chromatography, and the conversion rate of the xylo-oligosaccharide is calculated, and the specific results are shown in table 1.
The conversion of xylopentaose was × 100% xylopentaose content/total xylan content.
TABLE 1 Effect of Complex enzymes of the invention on conversion of xylopentaose
| Complex enzyme | Adding amount of | Conditions of enzymolysis | Content of xylopentasaccharide | Conversion rate of xylopentaose |
| Example 1 | 12g/Kg xylan | Enzymolysis at 50 deg.C for 12h | 20.1g/l | 40.2％ |
| Example 2 | 10.8g/Kg xylan | Enzymolysis at 60 deg.C for 6h | 20.6g/l | 41.2％ |
| Example 3 | 10g/Kg xylan | Enzymolysis at 55 deg.C for 8h | 21.0g/l | 42.0％ |
The results in table 1 show that the xylo-oligosaccharide solution prepared by treating the viscose squeezed liquid with the complex enzyme provided by the invention has the advantages that the content of xylo-oligosaccharide is remarkably improved, the conversion rate of the xylo-oligosaccharide is up to 42%, and unexpected effects are achieved.
Example 5 Effect of sodium carbonate addition on the conversion of xylopentaose
1. Design of experiments
Taking a hemicellulose solution with the xylan content of 50g/L prepared in the step 1 of the embodiment 4; adjusting the pH value of the hemicellulose solution to 5.5, respectively adding the following complex enzyme samples according to the proportion of 10g/Kg of xylan, and acting for 8 hours at 55 ℃; (ii) a After the enzymolysis reaction is finished, heating to 80 ℃, preserving the temperature for 15min, and inactivating the complex enzyme; and (3) carrying out solid-liquid separation on the solution after enzymolysis through a plate frame to obtain a clear solution, namely the xylo-oligosaccharide solution.
The compound enzyme sample formula comprises:
sample 1: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 0Kg of sodium carbonate.
Sample 2: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 0.5Kg of sodium carbonate.
Sample 3: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 1.0Kg of sodium carbonate.
Sample 4: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 1.5Kg of sodium carbonate.
Sample 5: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 2.0Kg of sodium carbonate.
Sample 6: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 2.5Kg of sodium carbonate.
Sample 7: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 3.0Kg of sodium carbonate.
Sample 8: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 3.5Kg of sodium carbonate.
Sample 9: 2Kg of xylanase, 0.5Kg of phytase, 0.2Kg of mannase and 4.0Kg of sodium carbonate.
2. Analysis of results
And respectively measuring the contents (g/l) of xylobiose, xylotriose, xylotetraose and xylopentaose in the xylooligosaccharide solution prepared by using the complex enzyme sample by using ion liquid chromatography, and calculating the conversion rate of the xylopentaose. The specific results are shown in Table 2.
TABLE 2 influence of the amount of sodium carbonate added on the conversion of xylopentaose
| Complex enzyme | Sodium carbonate | Xylobiose | Xylotriose | Wood tetrasaccharide | Wood sugar | Conversion rate of xylopentaose |
| Sample 1 | 0 | 5.5 | 4.0 | 5.4 | 12.5 | 25％ |
| Sample 2 | 0.5Kg | 5.5 | 4.0 | 5.5 | 13.0 | 26％ |
| Sample 3 | 1.0Kg | 5.5 | 4.0 | 5.5 | 13.0 | 26％ |
| Sample No. 4 | 1.5Kg | 5.8 | 3.8 | 5.3 | 15.8 | 31.6％ |
| Sample No. 5 | 2.0Kg | 6.0 | 4.4 | 5.0 | 20.2 | 40.2％ |
| Sample No. 6 | 2.5Kg | 6.2 | 4.8 | 4.8 | 21.8 | 43.6％ |
| Sample 7 | 3.0Kg | 5.8 | 4.0 | 5.0 | 21.0 | 42.0％ |
| Sample 8 | 3.5Kg | 6.0 | 3.8 | 5.3 | 16.2 | 32.4％ |
| Sample 9 | 4.0Kg | 5.8 | 4.2 | 5.0 | 15.2 | 30.4％ |
As can be seen from the results in table 2, sodium carbonate has the most significant effect on the conversion of xylopentaose compared to xylobiose, xylotriose and xylotetraose. With the increase of the addition amount of sodium carbonate in the complex enzyme preparation, the conversion rate of the xylo-oligosaccharide solution prepared by the complex enzyme preparation is slowly improved, but when the addition amount of sodium carbonate in the complex enzyme reaches 2.0Kg, the conversion rate of the xylo-oligosaccharide is greatly improved and exceeds 40%, and when the addition amount of sodium carbonate in the complex enzyme exceeds 3.0Kg, the conversion rate of the xylo-oligosaccharide begins to rapidly decrease. Therefore, when the addition amount of the sodium carbonate in the complex enzyme is 2.0-3.0Kg, the sodium carbonate can be synergistically acted with xylanase, phytase and mannase in the complex enzyme to promote the conversion of the xylopentaose, greatly improve the conversion rate of the xylopentaose, and obtain unexpected technical effects.
Example 6 application of xylooligosaccharide solution prepared by the invention in Bifidobacterium animalis culture
Research shows that the animal bifidobacterium can rapidly consume xylotetraose and xylopentaose in a 6-24 hour period in the process of metabolizing xylooligosaccharide, and xylobiose and xylotriose can be accumulated to a certain extent. The increase of the concentration of the sugar with the polymerization degree being equal to or larger than 4, especially the concentration of the xylopentaose can effectively improve the growth speed of the thalli and shorten the fermentation period.
The applicant cultures Bifidobacterium animalis using the xylo-oligosaccharide solution prepared in example 4 with a xylo-oligosaccharide content higher than 20g/l and a commercially available xylo-oligosaccharide product (with a xylo-oligosaccharide content lower than 5 g/l) as carbon source.
(1) Cultivation methodAnd (3) nutrient medium: basal medium (w/v): 0.5% of peptone; tryptone 0.5%; beef extract 0.5%; 1.0% of yeast powder; CaC120.0008％；MgSO40.0019％；KH2PO40.0004％；NHaCO30.04％；NaCl 0.008％。
(2) The culture conditions are as follows: culturing in 20ml threaded tube, standing at 37 deg.C for 0-48 hr, and measuring the cell density OD 600 of Bifidobacterium every 24 hr. The specific results are shown in Table 3,
table 3 effect of xylo-oligosaccharide on proliferating cell concentration of bifidobacterium animalis.
From the experimental results in table 3, it can be seen that, compared with the commercially available xylo-oligosaccharide products, when the xylo-oligosaccharide solution with the xylo-oligosaccharide higher than 20g/l provided by the invention is used as a carbon source to culture bifidobacterium animalis, the bacterial concentration of the bifidobacterium animalis can be significantly increased, and the bacterial concentration is respectively increased by 52% and 50.5% at 24 hours and 48 hours, so that the increase of the content of the xylo-oligosaccharide in the xylo-oligosaccharide can effectively promote the proliferation of the bifidobacterium animalis, shorten the bacterial culture period, reduce the production cost, increase the economic benefit and achieve significant effects.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (2)
1. The application of the special compound enzyme preparation in improving the conversion rate of the xylopentaose is characterized in that the special compound enzyme preparation comprises xylanase, phytase, mannase and sodium carbonate, wherein the weight ratio of the xylanase, the phytase, the mannase and the sodium carbonate is 2: 0.2-0.5: 0.5-0.2: 2-3;
the compound enzyme preparation is used for improving the conversion rate of the xylopentaose, and comprises the following specific steps:
1) performing membrane dealkalization and concentration on squeezed liquid produced by viscose fibers to obtain a hemicellulose solution, mixing dilute sulfuric acid with the concentration of 12% and the hemicellulose solution in equal volume, treating at the high temperature of 100 ℃ for 90min, and adjusting the pH value to be neutral by using calcium carbonate;
2) adjusting the pH value of the hemicellulose solution to 5.5 by using concentrated sulfuric acid, adding the complex enzyme preparation according to the proportion of 10-12g/Kg of xylan, and acting for 6-12h at 50-60 ℃;
3) after the enzymolysis reaction is finished, heating to more than 80 ℃, preserving the temperature for 15min, and inactivating the complex enzyme;
4) and (3) carrying out solid-liquid separation on the solution after enzymolysis through a plate frame to obtain a xylo-oligosaccharide solution.
2. The use according to claim 1, wherein the weight ratio of xylanase, phytase, mannanase and sodium carbonate is 2: 0.2-0.5: 0.5-0.2: 2.5.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438319A (en) * | 2003-03-25 | 2003-08-27 | 李全宏 | Production method of oligose and beverage containing same |
| CN1840674A (en) * | 2006-01-19 | 2006-10-04 | 江南大学 | Process for preparing xylo-oligosaccharide by enzymolysis of wheat bran |
| CN104894189A (en) * | 2015-07-06 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Method for extracting xylooligosaccharide from wheat bran |
| CN106755193A (en) * | 2016-12-01 | 2017-05-31 | 青岛澳蓝明东生物科技有限公司 | A kind of method for producing xylo-oligosaccharide as raw material with viscose rayon pressed liquor |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1438319A (en) * | 2003-03-25 | 2003-08-27 | 李全宏 | Production method of oligose and beverage containing same |
| CN1840674A (en) * | 2006-01-19 | 2006-10-04 | 江南大学 | Process for preparing xylo-oligosaccharide by enzymolysis of wheat bran |
| CN104894189A (en) * | 2015-07-06 | 2015-09-09 | 青岛嘉瑞生物技术有限公司 | Method for extracting xylooligosaccharide from wheat bran |
| CN106755193A (en) * | 2016-12-01 | 2017-05-31 | 青岛澳蓝明东生物科技有限公司 | A kind of method for producing xylo-oligosaccharide as raw material with viscose rayon pressed liquor |
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