CN111620965A - Preparation method of flammulina velutipes chitin - Google Patents
Preparation method of flammulina velutipes chitin Download PDFInfo
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- CN111620965A CN111620965A CN202010514885.2A CN202010514885A CN111620965A CN 111620965 A CN111620965 A CN 111620965A CN 202010514885 A CN202010514885 A CN 202010514885A CN 111620965 A CN111620965 A CN 111620965A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention belongs to the technical field of plant extraction, and particularly relates to a preparation method of flammulina velutipes chitin. The preparation method of the flammulina velutipes chitin provided by the invention adopts flammulina velutipes as a preparation raw material, and the flammulina velutipes chitin is prepared by the process steps of crushing, enzymolysis, protein removal, enzyme inactivation, purification, concentration under reduced pressure, freeze drying and the like. The extraction rate of the flammulina velutipes chitin extracted by the preparation method of the flammulina velutipes chitin provided by the invention reaches 12%, and the purity of the extracted flammulina velutipes chitin reaches 73.8%. In addition, the preparation method of the flammulina velutipes chitin provided by the invention is simple and easy to operate, has controllable conditions, and is easy to realize industrial production.
Description
Technical Field
The invention belongs to the technical field of plant extraction, and particularly relates to a preparation method of flammulina velutipes chitin.
Background
The famous flammulina velutipes is called as flammulina velutipes, also called as flammulina velutipes, because the stipe is slender and is similar to that of flammulina velutipes. The needle mushroom contains complete essential amino acid components, particularly abundant lysine and arginine, and high zinc content, and has good effect in enhancing intelligence, especially for children height and intelligence development. The needle mushroom also contains a substance called as needle mushroom essence, which has the effects of enhancing the resistance of the organism to cancer cells, reducing cholesterol, preventing liver diseases and gastrointestinal ulcer, enhancing the body vital qi, preventing diseases and building body after being eaten for a long time. The needle mushroom can effectively enhance the biological activity of organisms, promote metabolism in vivo, is beneficial to the absorption and utilization of various nutrient substances in things, and is also beneficial to growth and development. In addition, the golden mushroom can also inhibit blood fat from rising, reduce cholesterol, and prevent and treat cardiovascular and cerebrovascular diseases, the edible golden mushroom has the effects of resisting fatigue, resisting bacteria and diminishing inflammation, removing heavy metal salt substances and resisting tumors, and the golden mushroom can prevent and treat liver diseases and gastric and intestinal ulcers and is also suitable for being eaten by hypertension patients, obese people and middle-aged and old people.
The flammulina velutipes polysaccharide is a main active ingredient of flammulina velutipes, is a polymer formed by connecting more than 10 monosaccharides through glycosidic bonds, has various functions of good immunoregulation, tumor resistance, liver protection, memory enhancement and the like, and becomes a hotspot in the research fields of food science, natural products, biochemistry and life science. In addition, the flammulina velutipes polysaccharide also has excellent anti-fatigue effect and moisturizing function, and researches show that the flammulina velutipes polysaccharide has better capability of preventing water loss in the horny layer of the skin than glycerin.
The flammulina velutipes chitin is one of flammulina velutipes polysaccharides, exists in cell walls of flammulina velutipes, is a nitrogenous polysaccharide biopolymer, has the effects of bidirectional immune regulation and liver function strengthening, is nutritional and health-care, safe and free of toxic and side effects, is named as the sixth life element after five life elements of sugar, protein, lipid, vitamin and mineral substances are removed by the international medical nutrition food society, and is widely concerned. However, the extraction process of the flammulina velutipes chitin is difficult and heavy because the flammulina velutipes chitin is insoluble in water, dilute acid, alkali solution and organic solvent.
Patent application No. ZL200910045923.8 discloses a preparation method of chitosan in fungal cell walls, which comprises the steps of taking actinocor taiwanensis fungi as raw materials, obtaining bacterial liquid through scratching, shaking culture and fermentation culture, obtaining wet thalli through centrifugal separation, and obtaining the chitosan through separation, drying, crushing, alkali treatment, acid treatment and drying. The preparation method has low energy consumption, easy culture and extraction and simple process, but has low extraction efficiency, and the purity of the extracted chitosan can only reach 50 percent, and the comprehensive utilization effect is poor, thus being not beneficial to industrial production.
In conclusion, the prior art generally has the technical problems of difficult extraction, low extraction efficiency, low product purity, poor comprehensive utilization effect, difficult industrial production and the like.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a preparation method of flammulina velutipes chitin. The preparation method of the flammulina velutipes chitin provided by the invention has the advantages of simple and easy operation process, low extraction efficiency, high product purity, high comprehensive utilization rate, unnecessary waste avoidance, cost saving and easy realization of industrial production.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a preparation method of flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1-2cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 5-8 times of deionized water into the needle mushroom powder prepared in the step S1, soaking for 4-6 hours, heating to 40-60 ℃, adding complex enzyme, and performing enzymolysis for 1-2 hours to obtain an enzymolysis liquid;
s3, adding trichloroacetic acid into the enzymolysis liquid prepared in the step S2, stirring for 2-4 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 90-95 ℃, preserving heat for 0.5-1h, naturally cooling to 25 ℃, performing centrifugal separation, and performing reduced pressure concentration on supernatant to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction for 3-5 hours to prepare a mixed solution;
s6, concentrating the mixed solution prepared in the step S5 under reduced pressure, and freeze-drying to obtain the compound.
Further, the complex enzyme in the step S2 is composed of cellulase, pectinase and decarboxylase according to the mass ratio of 2-5:1-3: 3-5.
Further, the adding amount of the trichloroacetic acid in the step S3 is 5-10% of the mass of the enzymolysis liquid.
Further, the volume fraction of the ethanol solution in the step S5 is 85%, and the mass ratio of the dry extract to the ethanol solution is 1: 25-35.
Further, the vacuum degree of the vacuum concentration in the step S4 and the step S6 is-0.1 to-0.15 Mpa, and the temperature is 80 to 90 ℃.
The preparation method of the flammulina velutipes chitin provided by the invention adopts flammulina velutipes as a preparation raw material, and the flammulina velutipes chitin is prepared by the process steps of crushing, enzymolysis, protein removal, enzyme inactivation, purification, concentration under reduced pressure, freeze drying and the like. The cellulase and the pectinase in the complex enzyme can effectively break the cell wall of the flammulina velutipes, so that chitin in the cell wall of the flammulina velutipes is released, and a solid foundation is laid for extracting the flammulina velutipes chitin; the decarboxylase can remove amino acid contained in the flammulina velutipes, and is favorable for improving the purity of the extracted flammulina velutipes chitin. Trichloroacetic acid can form an insoluble salt with protein under acidic conditions, and the trichloroacetic acid can be used as a protein denaturant to change the conformation of the protein, expose more hydrophobic groups and enable the hydrophobic groups to aggregate and precipitate. Heating the supernatant without protein to 90-95 ℃ and preserving heat for 0.5-1h can effectively inactivate enzyme, thereby being beneficial to improving the purity of the flammulina velutipes chitin. And finally, carrying out ethanol reflux extraction on the solution, and removing monosaccharide, oligosaccharide and other small molecular substances in the solution through ethanol reflux in the process so as to further improve the purity of the flammulina velutipes chitin.
Compared with the prior art, the invention has the following advantages:
(1) the extraction rate of the flammulina velutipes chitin extracted by the preparation method of the flammulina velutipes chitin provided by the invention reaches 12%, and the purity of the extracted flammulina velutipes chitin reaches 73.8%;
(2) according to the preparation method of the flammulina velutipes chitin provided by the invention, the cell wall of the flammulina velutipes is effectively cracked by adopting cellulase and pectinase, and then amino acid and protein contained in the flammulina velutipes are removed by decarboxylase and trichloroacetic acid, so that the purity of the flammulina velutipes chitin is effectively improved;
(3) the preparation method of the flammulina velutipes chitin provided by the invention is simple and easy to operate, controllable in condition, stable in process and easy to realize industrial production.
Detailed Description
The present invention will be further described below by way of specific embodiments, but the present invention is not limited to only the following examples. Various modifications can be made by those skilled in the art based on the basic idea of the invention, but it is within the scope of the invention as long as it does not depart from the basic idea of the invention.
Example 1A Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 5 times of deionized water into the needle mushroom powder prepared in the step S1, soaking for 4 hours, heating to 40 ℃, adding a complex enzyme, performing enzymolysis, wherein the complex enzyme consists of cellulase, pectinase and decarboxylase according to a mass ratio of 2:1:3, and performing enzymolysis for 1 hour to prepare an enzymolysis liquid;
s3, adding trichloroacetic acid which accounts for 5% of the mass of the enzymolysis liquid into the enzymolysis liquid prepared in the step S2, stirring for 2 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 90 ℃, preserving heat for 0.5h, naturally cooling to 25 ℃, performing centrifugal separation, taking supernatant, and performing reduced pressure concentration, wherein the vacuum degree of the reduced pressure concentration is-0.1 Mpa, and the temperature is 80 ℃, so as to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction, wherein the volume fraction of the ethanol solution is 85%, the mass ratio of the dry extract to the ethanol solution is 1:25, and the reflux extraction time is 3 hours to prepare a mixed solution;
s6, carrying out reduced pressure concentration on the mixed solution prepared in the step S5, and freeze-drying, wherein the vacuum degree of the reduced pressure concentration is-0.1 Mpa, and the temperature is 80 ℃, thus obtaining the product.
Example 2A Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1.2cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 6 times of deionized water into the flammulina velutipes powder prepared in the step S1, soaking for 4.5 hours, heating to 45 ℃, adding a complex enzyme, performing enzymolysis on the complex enzyme consisting of cellulase, pectinase and decarboxylase according to a mass ratio of 3:2:5, and performing enzymolysis for 1.3 hours to obtain an enzymolysis liquid;
s3, adding trichloroacetic acid accounting for 6% of the mass of the enzymolysis liquid into the enzymolysis liquid prepared in the step S2, stirring for 2.5 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 91 ℃, preserving heat for 0.6h, naturally cooling to 25 ℃, performing centrifugal separation, taking supernatant, and performing reduced pressure concentration, wherein the vacuum degree of the reduced pressure concentration is-0.11 Mpa, and the temperature is 82 ℃, so as to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction, wherein the volume fraction of the ethanol solution is 85%, the mass ratio of the dry extract to the ethanol solution is 1:28, and the reflux extraction time is 3.5 hours to prepare a mixed solution;
s6, carrying out reduced pressure concentration on the mixed solution prepared in the step S5, and freeze-drying, wherein the vacuum degree of the reduced pressure concentration is-0.11 Mpa, and the temperature is 82 ℃, so that the preparation method is obtained.
Example 3A Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1.5cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 7.3 times of deionized water into the flammulina velutipes powder prepared in the step S1, soaking for 5 hours, heating to 51 ℃, adding a complex enzyme, performing enzymolysis on the complex enzyme consisting of cellulase, pectinase and decarboxylase according to the mass ratio of 4:3:5, and performing enzymolysis for 1.5 hours to obtain an enzymolysis liquid;
s3, adding trichloroacetic acid which accounts for 7% of the mass of the enzymolysis liquid into the enzymolysis liquid prepared in the step S2, stirring for 3 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 93 ℃, preserving heat for 0.7h, naturally cooling to 25 ℃, performing centrifugal separation, taking supernatant, and performing reduced pressure concentration, wherein the vacuum degree of the reduced pressure concentration is-0.13 Mpa, and the temperature is 85 ℃, so as to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction, wherein the volume fraction of the ethanol solution is 85%, the mass ratio of the dry extract to the ethanol solution is 1:30, and the reflux extraction time is 4 hours to prepare a mixed solution;
s6, carrying out reduced pressure concentration on the mixed solution prepared in the step S5, and freeze-drying, wherein the vacuum degree of the reduced pressure concentration is-0.13 Mpa, and the temperature is 85 ℃, so as to obtain the product.
Example 4A Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1.7cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 7 times of deionized water into the needle mushroom powder prepared in the step S1, soaking for 5.5 hours, heating to 55 ℃, adding a complex enzyme, performing enzymolysis on the complex enzyme consisting of cellulase, pectinase and decarboxylase according to a mass ratio of 5:3:4, wherein the enzymolysis time is 1.8 hours, and thus obtaining an enzymolysis liquid;
s3, adding trichloroacetic acid which accounts for 9% of the mass of the enzymolysis liquid into the enzymolysis liquid prepared in the step S2, stirring for 3.5 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 94 ℃, preserving heat for 0.8h, naturally cooling to 25 ℃, performing centrifugal separation, taking supernatant, and performing reduced pressure concentration, wherein the vacuum degree of the reduced pressure concentration is-0.14 Mpa, and the temperature is 87 ℃, so as to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction, wherein the volume fraction of the ethanol solution is 85%, the mass ratio of the dry extract to the ethanol solution is 1:32, and the reflux extraction time is 4.5 hours to prepare a mixed solution;
s6, carrying out reduced pressure concentration on the mixed solution prepared in the step S5, and freeze-drying, wherein the vacuum degree of the reduced pressure concentration is-0.14 Mpa, and the temperature is 87 ℃, so as to obtain the product.
Example 5A Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin specifically comprises the following steps:
s1, cutting the cleaned needle mushrooms to the length of 2cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 8 times of deionized water into the flammulina velutipes powder prepared in the step S1, soaking for 6 hours, heating to 60 ℃, adding a complex enzyme, performing enzymolysis, wherein the complex enzyme consists of cellulase, pectinase and decarboxylase according to a mass ratio of 3:1:4, and performing enzymolysis for 2 hours to obtain an enzymolysis liquid;
s3, adding trichloroacetic acid accounting for 10% of the mass of the enzymolysis liquid into the enzymolysis liquid prepared in the step S2, stirring for 4 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 95 ℃, preserving heat for 1 hour, naturally cooling to 25 ℃, centrifugally separating, taking supernatant fluid, and concentrating under reduced pressure, wherein the vacuum degree of the concentration under reduced pressure is-0.15 Mpa, and the temperature is 90 ℃, so as to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction, wherein the volume fraction of the ethanol solution is 85%, the mass ratio of the dry extract to the ethanol solution is 1:35, and the reflux extraction time is 5 hours to prepare a mixed solution;
s6, carrying out reduced pressure concentration on the mixed solution prepared in the step S5, and freeze-drying, wherein the vacuum degree of the reduced pressure concentration is-0.15 Mpa, and the temperature is 90 ℃, so as to obtain the product.
Comparative example 1 Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin is similar to that of example 3.
The difference between this comparative example and example 3 is: the complex enzyme in the step S2 in the comparative example consists of cellulase, pectinase and decarboxylase according to the mass ratio of 1:1: 1.
Comparative example 2 Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin is similar to that of example 3.
The difference between this comparative example and example 3 is: the amount of trichloroacetic acid added in step S3 described in this comparative example was 15% by mass of the enzymatic hydrolysate.
Comparative example 3 Flammulina velutipes chitin
The preparation method of the flammulina velutipes chitin is similar to that of example 3.
The difference between this comparative example and example 3 is: the mass ratio of the dry extract to the ethanol solution in the step S5 in the comparative example is 1: 15.
Test example, detection of content of Flammulina velutipes chitin
Test samples: flammulina velutipes chitin obtained in examples 1 to 5 and comparative examples 1 to 3;
the test method comprises the following steps: preparing a test sample into a solution with the molar concentration of 0.05mol/L, fixing the volume, and measuring the extraction rate and the content of the chitin by adopting a sulfuric acid-phenol method;
and (3) test results: the test results are shown in Table 1.
TABLE 1 detection results of the content of chitin in Flammulina velutipes
| Group of | Percent extraction of flammulina velutipes polysaccharide | Content of Flammulina velutipes polysaccharide% |
| Example 1 | 12.7 | 74.1 |
| Example 2 | 12.3 | 73.8 |
| Example 3 | 13.4 | 75.3 |
| Example 4 | 12.2 | 73.9 |
| Example 5 | 12.5 | 74.6 |
| Comparative example 1 | 7.4 | 58.4 |
| Comparative example 2 | 7.1 | 59.1 |
| Comparative example 3 | 6.9 | 58.7 |
As can be seen from Table 1, the preparation method of the flammulina velutipes chitin provided by the invention can realize the extraction of the flammulina velutipes chitin with the content of more than 12%, and the purity of the flammulina velutipes chitin with the purity of more than 73.8%, and has great progress compared with the prior art. The best embodiment of the invention is that the extraction rate of the flammulina velutipes chitin prepared in the embodiment 3 is highest, and the purity is the best.
Compared with the embodiment 3, the comparative example 1 changes the addition proportion of the complex enzyme, but the prepared flammulina velutipes chitin has lower purity and lower extraction rate, which shows that the proportion of the complex enzyme in the preparation method of the flammulina velutipes chitin provided by the invention reaches the optimal proportion; the comparative example 2 increases the consumption of trichloroacetic acid, but the extraction rate of the flammulina velutipes chitin and the purity of the flammulina velutipes chitin are obviously lower than those in the example 3, which is caused by excessive consumption of trichloroacetic acid, and excessive trichloroacetic acid not only can reduce the combination rate of the flammulina velutipes chitin and protein, but also can cause the extraction rate of the flammulina velutipes chitin to be reduced and the purity of the flammulina velutipes chitin to be reduced because trichloroacetic acid impurities are contained in the flammulina velutipes chitin; comparative example 3 reduces the mass ratio of the dry extract to the ethanol solution, but the extraction rate of the flammulina velutipes chitin and the purity of the flammulina velutipes chitin are obviously lower than those in example 3, because the dosage of the ethanol solution is too low, small molecular substances are incompletely extracted in the ethanol reflux extraction process, and the extraction rate of the flammulina velutipes chitin and the purity of the flammulina velutipes chitin are reduced.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Those skilled in the art will recognize that changes may be made to the embodiments described above without departing from the spirit and scope of the invention. Therefore, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the technical spirit of the present invention are covered by the claims of the present invention.
Claims (5)
1. A preparation method of flammulina velutipes chitin is characterized by comprising the following steps:
s1, cutting the cleaned needle mushrooms to the length of 1-2cm, freeze-drying and grinding to 10 microns to obtain needle mushroom powder;
s2, adding 5-8 times of deionized water into the needle mushroom powder prepared in the step S1, soaking for 4-6 hours, heating to 40-60 ℃, adding complex enzyme, and performing enzymolysis for 1-2 hours to obtain an enzymolysis liquid;
s3, adding trichloroacetic acid into the enzymolysis liquid prepared in the step S2, stirring for 2-4 hours under the condition of 400 revolutions per minute until no precipitate is generated, and filtering to prepare filtrate;
s4, heating the filtrate obtained in the step S3 to 90-95 ℃, preserving heat for 0.5-1h, naturally cooling to 25 ℃, performing centrifugal separation, and performing reduced pressure concentration on supernatant to obtain dry extract;
s5, adding an ethanol solution into the dry extract prepared in the step S4 for reflux extraction for 3-5 hours to prepare a mixed solution;
s6, concentrating the mixed solution prepared in the step S5 under reduced pressure, and freeze-drying to obtain the compound.
2. The method for preparing flammulina velutipes chitin according to claim 1, wherein the complex enzyme in step S2 is composed of cellulase, pectinase and decarboxylase in a mass ratio of 2-5:1-3: 3-5.
3. The method for preparing flammulina velutipes chitin according to claim 1, wherein the amount of trichloroacetic acid added in step S3 is 5-10% of the mass of the enzymatic hydrolysate.
4. The preparation method of flammulina velutipes chitin according to claim 1, wherein the volume fraction of the ethanol solution in step S5 is 85%, and the mass ratio of the dry extract to the ethanol solution is 1: 25-35.
5. The method of preparing Flammulina velutipes chitin according to claim 1, wherein the vacuum degree of the vacuum concentration in the steps S4 and S6 is-0.1 to-0.15 MPa, and the temperature is 80 to 90 ℃.
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| CN115381087A (en) * | 2022-06-22 | 2022-11-25 | 深圳市维龄可伴生物科技有限公司 | Efficient extraction process and application of needle mushroom powder |
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| CN106478838A (en) * | 2016-12-02 | 2017-03-08 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of extracting method of high-purity edible fungi polysaccharide |
| CN108659145A (en) * | 2018-06-19 | 2018-10-16 | 苏州汉德瑞生物工程有限公司 | The method of extraction fungi chitosan and application in a kind of mushroom |
Cited By (1)
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| CN115381087A (en) * | 2022-06-22 | 2022-11-25 | 深圳市维龄可伴生物科技有限公司 | Efficient extraction process and application of needle mushroom powder |
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