CN1610754B - 具有脱卤素酶活性的酶及其使用方法 - Google Patents

具有脱卤素酶活性的酶及其使用方法 Download PDF

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Publication number
CN1610754B
CN1610754B CN018222587A CN01822258A CN1610754B CN 1610754 B CN1610754 B CN 1610754B CN 018222587 A CN018222587 A CN 018222587A CN 01822258 A CN01822258 A CN 01822258A CN 1610754 B CN1610754 B CN 1610754B
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China
Prior art keywords
sequences
polypeptide
nucleic acid
sequence
dehalogenase
Prior art date
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Expired - Fee Related
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CN018222587A
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English (en)
Chinese (zh)
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CN1610754A (zh
Inventor
J·M·肖特
T·理查森
D·罗伯逊
K·格雷
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BASF Enzymes LLC
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Verenium Corp
Diversa Corp
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Publication of CN1610754A publication Critical patent/CN1610754A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S422/00—Chemical apparatus and process disinfecting, deodorizing, preserving, or sterilizing
    • Y10S422/90—Decreasing pollution or environmental impact

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN018222587A 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法 Expired - Fee Related CN1610754B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25089700P 2000-12-01 2000-12-01
US60/250,897 2000-12-01
PCT/US2001/045337 WO2002068583A2 (en) 2000-12-01 2001-11-30 Enzymes having dehalogenase activity and methods of use thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN2012101536411A Division CN102690799A (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Publications (2)

Publication Number Publication Date
CN1610754A CN1610754A (zh) 2005-04-27
CN1610754B true CN1610754B (zh) 2012-07-04

Family

ID=22949600

Family Applications (2)

Application Number Title Priority Date Filing Date
CN018222587A Expired - Fee Related CN1610754B (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法
CN2012101536411A Pending CN102690799A (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Family Applications After (1)

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CN2012101536411A Pending CN102690799A (zh) 2000-12-01 2001-11-30 具有脱卤素酶活性的酶及其使用方法

Country Status (9)

Country Link
US (4) US7078504B2 (https=)
EP (1) EP1507870A4 (https=)
JP (4) JP4589602B2 (https=)
KR (1) KR20030051897A (https=)
CN (2) CN1610754B (https=)
BR (1) BR0115875A (https=)
CA (2) CA2430192C (https=)
MX (1) MXPA03004840A (https=)
WO (1) WO2002068583A2 (https=)

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Publication number Priority date Publication date Assignee Title
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
WO2000040715A2 (en) * 1999-01-05 2000-07-13 Trustees Of Boston University Improved nucleic acid cloning
US7435562B2 (en) * 2000-07-21 2008-10-14 Modular Genetics, Inc. Modular vector systems
MXPA03004840A (es) 2000-12-01 2008-10-08 Verenium Corp Enzimas que tienen actividad de deshalogenasa y metodos.
AU2003263031A1 (en) 2002-09-20 2004-04-08 Diversa Corporation Chemoenzymatic methods for the synthesis of statins and stain intermediates
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
EP1817413B1 (en) 2004-11-11 2012-01-04 Modular Genetics, Inc. Oligonucleotide ladder assembly and system for generating molecular diversity
US7632666B2 (en) * 2004-12-27 2009-12-15 Masarykova Univerzita V Brne Method of production of optically active halohydrocarbons and alcohols using hydrolytic dehalogenation catalysed by haloalkane dehalogenases
EP2374875B1 (en) 2006-10-30 2015-09-02 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and funcitional expression
WO2011137368A2 (en) 2010-04-30 2011-11-03 Life Technologies Corporation Systems and methods for analyzing nucleic acid sequences
US9268903B2 (en) 2010-07-06 2016-02-23 Life Technologies Corporation Systems and methods for sequence data alignment quality assessment
CN102296046B (zh) * 2011-09-16 2013-08-14 广州市暨源生物科技有限公司 可用于预防慢性重金属中毒的重组食品级乳酸菌、其制备方法与应用
PL3107554T3 (pl) * 2014-02-19 2020-12-28 University Of Tennessee Research Foundation Przeciwciało dla odchylenia proporcji płci i sposoby jego zastosowania
CN104694514B (zh) * 2015-03-18 2017-11-28 中国科学院微生物研究所 一种脱卤酶DhmB及其编码基因与应用
EP3093337A3 (en) 2015-05-13 2017-03-15 Samsung Electronics Co., Ltd. Microorganism including gene encoding protein having hydroxylase activity and method of reducing concentration of fluorinated methane in sample using the same
US10358632B2 (en) 2015-12-07 2019-07-23 Samsung Electronics Co., Ltd. Bacterial cytochrome P450 protein variant and method of reducing concentration of fluorinated methane in sample using the same
CN109706139A (zh) * 2019-03-01 2019-05-03 中国人民解放军92609部队 脱卤素酶基因LinB、脱卤素酶、脱卤素酶基因工程菌及其构建方法和应用方法
CN114574360B (zh) * 2020-12-01 2025-02-11 中国科学院大连化学物理研究所 基于石英内衬管阵列的细胞操作装置及方法
US11760793B2 (en) 2020-12-18 2023-09-19 University Of Tennessee Research Foundation Antibody for skewing sex ratio and methods of use thereof
CN120924518B (zh) * 2025-08-06 2026-04-21 湖南师范大学 一种卤代烷烃脱卤酶hld-1405及其编码基因与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252100A (zh) * 1997-02-13 2000-05-03 唐化学原料公司 重组卤代脂族脱卤素酶

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US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US6037465A (en) * 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
EP1117780A2 (en) * 1998-09-29 2001-07-25 Diversa Corporation Nucleic acids and proteins from cenarchaeum symbiosum
JP2004500019A (ja) * 1999-03-26 2004-01-08 ディベルサ　コーポレーション 定方向進化におけるエキソヌクレアーゼ仲介核酸再集合
WO2002022810A2 (en) * 2000-09-15 2002-03-21 Diversa Corporation Combinatorial screening of mixed populations of organisms
MXPA03004840A (es) 2000-12-01 2008-10-08 Verenium Corp Enzimas que tienen actividad de deshalogenasa y metodos.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1252100A (zh) * 1997-02-13 2000-05-03 唐化学原料公司 重组卤代脂族脱卤素酶

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Gerrit J. Poelarends, et al.Degradation of 1,2-Dibromoethane by Mycobacterium sp.Strain GP1.Journal of Bacteriology181 7.1999,图5.
Gerrit J. Poelarends, et al.Degradation of 1,2-Dibromoethane by Mycobacterium sp.Strain GP1.Journal of Bacteriology181 7.1999,图5. *
Newman J et al.Haloalkane Dehalogenases"structure of A Rhodococcusenzyme.Biochemistry,American Chemical Society38.1999,第16106页右栏. *

Also Published As

Publication number Publication date
JP2005501515A (ja) 2005-01-20
JP2015107123A (ja) 2015-06-11
JP4589602B2 (ja) 2010-12-01
EP1507870A2 (en) 2005-02-23
JP5697365B2 (ja) 2015-04-08
BR0115875A (pt) 2005-05-10
US20130115675A1 (en) 2013-05-09
CN1610754A (zh) 2005-04-27
US7671189B2 (en) 2010-03-02
CA2430192A1 (en) 2002-09-06
US8153411B2 (en) 2012-04-10
US20060035216A1 (en) 2006-02-16
US20060275804A1 (en) 2006-12-07
CN102690799A (zh) 2012-09-26
WO2002068583A2 (en) 2002-09-06
CA2430192C (en) 2014-06-03
US20100112666A1 (en) 2010-05-06
WO2002068583A3 (en) 2004-12-02
US7078504B2 (en) 2006-07-18
CA2846761A1 (en) 2002-09-06
EP1507870A4 (en) 2005-11-09
KR20030051897A (ko) 2003-06-25
US9453211B2 (en) 2016-09-27
JP2010268796A (ja) 2010-12-02
MXPA03004840A (es) 2008-10-08
JP2008142089A (ja) 2008-06-26

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Owner name: WIRAINIM CO.,LTD.

Free format text: FORMER OWNER: DIVERSA CORP.

Effective date: 20091113

C41 Transfer of patent application or patent right or utility model
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Address after: American California

Applicant after: Diversa Corp.

Address before: American California

Applicant before: Diversa Corp.

C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee
CP01 Change in the name or title of a patent holder

Address after: American California

Patentee after: BASF Enzymes LLC

Address before: American California

Patentee before: Diversa Corp.

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120704

Termination date: 20161130