CN1610754B - 具有脱卤素酶活性的酶及其使用方法 - Google Patents
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
Description
Claims (36)
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US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
CA2360011A1 (en) * | 1999-01-05 | 2000-07-13 | Trustees Of Boston University | Ordered gene assembly |
US7435562B2 (en) * | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
KR20030051897A (ko) | 2000-12-01 | 2003-06-25 | 다이버사 코포레이션 | 데할로게나제 활성을 가진 효소 및 이의 사용 방법 |
EP1625223A4 (en) * | 2002-09-20 | 2009-11-11 | Verenium Corp | CHEMOENZYMATIC PROCEDURES FOR SYNTHESIS OF STATINES AND STATIN INTERCONNECTIONS |
US7238842B2 (en) | 2003-01-31 | 2007-07-03 | Promega Corporation | Covalent tethering of functional groups to proteins |
US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
JP2008519602A (ja) | 2004-11-11 | 2008-06-12 | モジュラー ジェネティクス, インコーポレイテッド | 多様性を生じるためのラダーアセンブリおよびシステム |
US7632666B2 (en) * | 2004-12-27 | 2009-12-15 | Masarykova Univerzita V Brne | Method of production of optically active halohydrocarbons and alcohols using hydrolytic dehalogenation catalysed by haloalkane dehalogenases |
EP2087107A2 (en) * | 2006-10-30 | 2009-08-12 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
WO2011137368A2 (en) | 2010-04-30 | 2011-11-03 | Life Technologies Corporation | Systems and methods for analyzing nucleic acid sequences |
US9268903B2 (en) | 2010-07-06 | 2016-02-23 | Life Technologies Corporation | Systems and methods for sequence data alignment quality assessment |
CN102296046B (zh) * | 2011-09-16 | 2013-08-14 | 广州市暨源生物科技有限公司 | 可用于预防慢性重金属中毒的重组食品级乳酸菌、其制备方法与应用 |
CN104694514B (zh) * | 2015-03-18 | 2017-11-28 | 中国科学院微生物研究所 | 一种脱卤酶DhmB及其编码基因与应用 |
EP3093337A3 (en) | 2015-05-13 | 2017-03-15 | Samsung Electronics Co., Ltd. | Microorganism including gene encoding protein having hydroxylase activity and method of reducing concentration of fluorinated methane in sample using the same |
US10358632B2 (en) | 2015-12-07 | 2019-07-23 | Samsung Electronics Co., Ltd. | Bacterial cytochrome P450 protein variant and method of reducing concentration of fluorinated methane in sample using the same |
CN109706139A (zh) * | 2019-03-01 | 2019-05-03 | 中国人民解放军92609部队 | 脱卤素酶基因LinB、脱卤素酶、脱卤素酶基因工程菌及其构建方法和应用方法 |
US11760793B2 (en) | 2020-12-18 | 2023-09-19 | University Of Tennessee Research Foundation | Antibody for skewing sex ratio and methods of use thereof |
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CN1252100A (zh) * | 1997-02-13 | 2000-05-03 | 唐化学原料公司 | 重组卤代脂族脱卤素酶 |
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US5108892A (en) * | 1989-08-03 | 1992-04-28 | Promega Corporation | Method of using a taq dna polymerase without 5'-3'-exonuclease activity |
US6037465A (en) * | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
US5723125A (en) * | 1995-12-28 | 1998-03-03 | Tanox Biosystems, Inc. | Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide |
US6183740B1 (en) * | 1997-08-13 | 2001-02-06 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
AU6504099A (en) * | 1998-09-29 | 2000-04-17 | Diversa Corporation | Nucleic acids and proteins from Cenarchaeum symbiosum |
EP1092041A4 (en) * | 1999-03-26 | 2001-09-19 | Diversa Corp | EXONUCLEASE-MEDIATED ASSEMBLY OF NUCLEIC ACIDS IN THE DIRECT EVOLUTION |
EP1319068A2 (en) | 2000-09-15 | 2003-06-18 | Diversa Corporation | Combinatorial screening of mixed populations of organisms |
KR20030051897A (ko) | 2000-12-01 | 2003-06-25 | 다이버사 코포레이션 | 데할로게나제 활성을 가진 효소 및 이의 사용 방법 |
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Patent Citations (1)
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CN1252100A (zh) * | 1997-02-13 | 2000-05-03 | 唐化学原料公司 | 重组卤代脂族脱卤素酶 |
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Gerrit J. Poelarends, et al.Degradation of 1,2-Dibromoethane by Mycobacterium sp.Strain GP1.Journal of Bacteriology181 7.1999,图5. * |
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JP2008142089A (ja) | 2008-06-26 |
US20060035216A1 (en) | 2006-02-16 |
US20060275804A1 (en) | 2006-12-07 |
CA2430192C (en) | 2014-06-03 |
KR20030051897A (ko) | 2003-06-25 |
US9453211B2 (en) | 2016-09-27 |
US7671189B2 (en) | 2010-03-02 |
JP2005501515A (ja) | 2005-01-20 |
EP1507870A2 (en) | 2005-02-23 |
CN102690799A (zh) | 2012-09-26 |
US20130115675A1 (en) | 2013-05-09 |
CN1610754A (zh) | 2005-04-27 |
JP2015107123A (ja) | 2015-06-11 |
WO2002068583A2 (en) | 2002-09-06 |
CA2430192A1 (en) | 2002-09-06 |
MXPA03004840A (es) | 2008-10-08 |
CA2846761A1 (en) | 2002-09-06 |
US8153411B2 (en) | 2012-04-10 |
BR0115875A (pt) | 2005-05-10 |
US7078504B2 (en) | 2006-07-18 |
JP5697365B2 (ja) | 2015-04-08 |
JP4589602B2 (ja) | 2010-12-01 |
WO2002068583A3 (en) | 2004-12-02 |
JP2010268796A (ja) | 2010-12-02 |
EP1507870A4 (en) | 2005-11-09 |
US20100112666A1 (en) | 2010-05-06 |
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