CN1610738A - 诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法 - Google Patents

诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法 Download PDF

Info

Publication number
CN1610738A
CN1610738A CNA028265998A CN02826599A CN1610738A CN 1610738 A CN1610738 A CN 1610738A CN A028265998 A CNA028265998 A CN A028265998A CN 02826599 A CN02826599 A CN 02826599A CN 1610738 A CN1610738 A CN 1610738A
Authority
CN
China
Prior art keywords
cell
stem cell
cells
mesodermal stem
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA028265998A
Other languages
English (en)
Chinese (zh)
Inventor
本望修
滨田洋文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RENOMEDIX Inst Inc
Original Assignee
RENOMEDIX Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RENOMEDIX Inst Inc filed Critical RENOMEDIX Inst Inc
Publication of CN1610738A publication Critical patent/CN1610738A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/02Drugs for disorders of the nervous system for peripheral neuropathies
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/08Antiepileptics; Anticonvulsants
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/18Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/28Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/44Thiols, e.g. mercaptoethanol
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1353Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/04Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Psychiatry (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Cardiology (AREA)
  • Hospice & Palliative Care (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CNA028265998A 2001-10-30 2002-10-30 诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法 Pending CN1610738A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
PCT/JP2001/009510 WO2003038074A1 (fr) 2001-10-30 2001-10-30 Procede d'induction de differenciation de cellules souches de mesoblastes ou de cellules es dans des cellules du systeme nerveux
JPPCT/JP01/09510 2001-10-30
JPPCT/JP02/03344 2002-04-03
PCT/JP2002/003344 WO2003038090A1 (fr) 2001-10-30 2002-04-03 Procede d'induction de differentiation de cellules souches mesodermiques immortalisees dans les cellules du systeme nerveux

Publications (1)

Publication Number Publication Date
CN1610738A true CN1610738A (zh) 2005-04-27

Family

ID=11737884

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA028265998A Pending CN1610738A (zh) 2001-10-30 2002-10-30 诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法

Country Status (4)

Country Link
JP (1) JP4118236B2 (ko)
KR (1) KR101022349B1 (ko)
CN (1) CN1610738A (ko)
WO (2) WO2003038074A1 (ko)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101294146B (zh) * 2007-04-28 2011-05-18 中国科学院上海生命科学研究院 诱导神经干细胞分化的系统及诱导方法
CN112746057A (zh) * 2020-12-25 2021-05-04 中国人民解放军海军军医大学 于体外将人多能干细胞诱导为神经中胚层祖细胞并维持自我更新的培养体系、方法及应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2413910A1 (en) 2000-06-26 2002-12-27 Renomedix Institute Inc. Cell fractions containing cells capable of differentating into neural cells
KR101540698B1 (ko) 2012-10-05 2015-07-30 사회복지법인 삼성생명공익재단 허혈혈청을 포함하는 줄기세포 활성화 촉진용 조성물 및 줄기세포의 활성화 촉진 방법
US10071144B2 (en) 2013-02-06 2018-09-11 Nc Medical Research Inc. Cell therapy for the treatment of neurodegeneration

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1254952A4 (en) * 1999-12-28 2004-09-22 Kyowa Hakko Kogyo Kk CELLS WHICH CAN INDUCE DIFFERENTIATION IN CARDIAC MUSCLE CELLS

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101294146B (zh) * 2007-04-28 2011-05-18 中国科学院上海生命科学研究院 诱导神经干细胞分化的系统及诱导方法
CN112746057A (zh) * 2020-12-25 2021-05-04 中国人民解放军海军军医大学 于体外将人多能干细胞诱导为神经中胚层祖细胞并维持自我更新的培养体系、方法及应用

Also Published As

Publication number Publication date
JP4118236B2 (ja) 2008-07-16
WO2003038090A1 (fr) 2003-05-08
JPWO2003038075A1 (ja) 2005-02-24
KR20040062610A (ko) 2004-07-07
WO2003038074A1 (fr) 2003-05-08
KR101022349B1 (ko) 2011-03-22

Similar Documents

Publication Publication Date Title
CA2465653C (en) Method for inducing differentiation of mesodermal stem cells, es cells, or immortalized mesodermal stem cells into neural cells
US7807461B2 (en) Multipotent stem cells derived from human adipose tissue and cellular therapeutic agents comprising the same
US8093047B2 (en) Induction of myocardial cell from mammalian bone marrow cell or cord blood-derived cell and fat tissue
CN1636054A (zh) 来自人胚胎干细胞的间充质细胞和成骨细胞
JP7055638B2 (ja) 幹細胞からの筋肉系列細胞の生成
CN1860222A (zh) 用于临床和商业用途的干细胞
CN103930542A (zh) 棕色脂肪细胞组合物及方法
JP2004057198A (ja) 多能性体細胞
WO2010069204A1 (zh) 亚全能干细胞、其制备方法及其用途
RU2433172C2 (ru) Способ получения гомогенной популяции стволовых клеток и ее применение
US20090162326A1 (en) Method for isolating stem cells and stem cells derived from a pad-like tissue of teeth
JP2004511266A (ja) 間葉間質細胞に対する治療的利用法
CN1961068A (zh) 骨髓基质细胞培养神经干细胞的方法和组合物
CN109628388B (zh) 用消化酶组合物从胎盘血管分离间充质干细胞
CN1778905A (zh) 一种脂肪间充质干细胞的分离培养方法及其应用
JPWO2006028049A1 (ja) アストロサイト様細胞馴化培地の製造方法
CN1610738A (zh) 诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法
US20040208858A1 (en) Therapeutic uses for mesenchymal stromal cells
CN114214272A (zh) 一种诱导脐带间充质干细胞分化心肌细胞的方法、培养基及其应用
KR101738715B1 (ko) 식물 줄기세포 또는 식물 역분화 줄기 세포의 추출물을 이용한 맞춤형 만능줄기세포의 유도 방법 및 상기 방법에 의해 제조된 만능줄기세포
WO2013123607A1 (zh) 用于成体干细胞的体外无血清培养的方法和培养基
JP2022540579A (ja) cP1Pまたはその薬学的に許容可能な塩を有効成分として含む幹細胞増殖促進用組成物
JP2021518118A (ja) 神経幹細胞の高効率分離培養方法
Guan et al. Ischemia, immunosuppression, and SSEA‐1‐negative cells all contribute to tumors resulting from mouse embryonic stem cell‐derived neural progenitor transplantation
WO2022244502A1 (ja) 皮膚付属器誘導能を有する細胞、及びその製造方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication