WO2003038090A1 - Procede d'induction de differentiation de cellules souches mesodermiques immortalisees dans les cellules du systeme nerveux - Google Patents

Procede d'induction de differentiation de cellules souches mesodermiques immortalisees dans les cellules du systeme nerveux Download PDF

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Publication number
WO2003038090A1
WO2003038090A1 PCT/JP2002/003344 JP0203344W WO03038090A1 WO 2003038090 A1 WO2003038090 A1 WO 2003038090A1 JP 0203344 W JP0203344 W JP 0203344W WO 03038090 A1 WO03038090 A1 WO 03038090A1
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Prior art keywords
cells
nervous system
stem cells
mesodermal stem
diffrentiation
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Application number
PCT/JP2002/003344
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English (en)
French (fr)
Inventor
Osamu Honmou
Hirofumi Hamada
Original Assignee
Renomedix Institute Inc.
Teikoku Hormone Mfg. Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Renomedix Institute Inc., Teikoku Hormone Mfg. Co., Ltd. filed Critical Renomedix Institute Inc.
Priority to US10/493,964 priority Critical patent/US20040259254A1/en
Priority to CNA028265998A priority patent/CN1610738A/zh
Priority to PCT/JP2002/011294 priority patent/WO2003038075A1/ja
Priority to EP02775435A priority patent/EP1452586B1/en
Priority to KR1020047006637A priority patent/KR101022349B1/ko
Priority to CA2465653A priority patent/CA2465653C/en
Priority to JP2003540340A priority patent/JP4118236B2/ja
Publication of WO2003038090A1 publication Critical patent/WO2003038090A1/ja
Priority to JP2007319328A priority patent/JP2008119003A/ja
Priority to US12/437,639 priority patent/US20090280564A1/en
Priority to JP2009278621A priority patent/JP2010051326A/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/08—Antiepileptics; Anticonvulsants
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1353—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from bone marrow mesenchymal stem cells (BM-MSC)
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    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

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  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Psychiatry (AREA)
  • Pain & Pain Management (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Rheumatology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PCT/JP2002/003344 2001-10-30 2002-04-03 Procede d'induction de differentiation de cellules souches mesodermiques immortalisees dans les cellules du systeme nerveux WO2003038090A1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
US10/493,964 US20040259254A1 (en) 2001-10-30 2002-10-30 Method for inducing differentiation mesodermal stem cells, es cells or immortalized cells into nervous system cells
CNA028265998A CN1610738A (zh) 2001-10-30 2002-10-30 诱导中胚层干细胞,es细胞,或无限增殖化的中胚层干细胞分化成神经细胞的方法
PCT/JP2002/011294 WO2003038075A1 (fr) 2001-10-30 2002-10-30 Procede permettant d'induire une differenciation de cellules souches embroyonnaires mesodermiques, de cellules es ou de cellules immortalisees dans des cellules du systeme nerveux
EP02775435A EP1452586B1 (en) 2001-10-30 2002-10-30 Method of inducing differentiation of mesodermal stem cells into nervous system cells
KR1020047006637A KR101022349B1 (ko) 2001-10-30 2002-10-30 중배엽간세포 혹은 ｅｓ세포, 또는 불사화한중배엽간세포로부터 신경계세포로의 분화유도 방법
CA2465653A CA2465653C (en) 2001-10-30 2002-10-30 Method for inducing differentiation of mesodermal stem cells, es cells, or immortalized mesodermal stem cells into neural cells
JP2003540340A JP4118236B2 (ja) 2001-10-30 2002-10-30 中胚葉幹細胞もしくはｅｓ細胞、または不死化した中胚葉幹細胞から神経系細胞への分化誘導方法
JP2007319328A JP2008119003A (ja) 2001-10-30 2007-12-11 中胚葉幹細胞もしくはｅｓ細胞、または不死化した中胚葉幹細胞から神経系細胞への分化誘導方法
US12/437,639 US20090280564A1 (en) 2001-10-30 2009-05-08 Method for inducing differentiation of mesodermal stem cells, es cells, or immortalized mesodermal stem cells into neural cells
JP2009278621A JP2010051326A (ja) 2001-10-30 2009-12-08 中胚葉幹細胞を神経系細胞に分化誘導する方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/JP2001/009510 WO2003038074A1 (fr) 2001-10-30 2001-10-30 Procede d'induction de differenciation de cellules souches de mesoblastes ou de cellules es dans des cellules du systeme nerveux
JPPCT/JP01/09510 2001-10-30

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WO2003038090A1 true WO2003038090A1 (fr) 2003-05-08

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PCT/JP2001/009510 WO2003038074A1 (fr) 2001-10-30 2001-10-30 Procede d'induction de differenciation de cellules souches de mesoblastes ou de cellules es dans des cellules du systeme nerveux
PCT/JP2002/003344 WO2003038090A1 (fr) 2001-10-30 2002-04-03 Procede d'induction de differentiation de cellules souches mesodermiques immortalisees dans les cellules du systeme nerveux

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Application Number Title Priority Date Filing Date
PCT/JP2001/009510 WO2003038074A1 (fr) 2001-10-30 2001-10-30 Procede d'induction de differenciation de cellules souches de mesoblastes ou de cellules es dans des cellules du systeme nerveux

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JP (1) JP4118236B2 (ja)
KR (1) KR101022349B1 (ja)
CN (1) CN1610738A (ja)
WO (2) WO2003038074A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7098027B2 (en) 2000-06-26 2006-08-29 Renomedix Institute, Inc. Method for remyelinating a demyelinized lesion due to injury in the brain or spinal cord
US10071144B2 (en) 2013-02-06 2018-09-11 Nc Medical Research Inc. Cell therapy for the treatment of neurodegeneration

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101294146B (zh) * 2007-04-28 2011-05-18 中国科学院上海生命科学研究院 诱导神经干细胞分化的系统及诱导方法
US10072247B2 (en) 2012-10-05 2018-09-11 Samsung Life Public Welfare Foundation Composition comprising ischemic serum for promoting activation of stem cell and method for promoting activation of stem cell
CN112746057A (zh) * 2020-12-25 2021-05-04 中国人民解放军海军军医大学 于体外将人多能干细胞诱导为神经中胚层祖细胞并维持自我更新的培养体系、方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048151A1 (fr) * 1999-12-28 2001-07-05 Kyowa Hakko Kogyo Co., Ltd. Cellules pouvant induire une differenciation dans des cellules du muscle cardiaque

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001048151A1 (fr) * 1999-12-28 2001-07-05 Kyowa Hakko Kogyo Co., Ltd. Cellules pouvant induire une differenciation dans des cellules du muscle cardiaque

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
KOBAYASHI N. ET AL.: "Retroviral transduction of human bone marrow mesenchymal stem cells with hTERT for tissue engineering", CELL TRANSPLANTATION, vol. 10, no. 6, 14 October 2001 (2001-10-14) - 17 October 2001 (2001-10-17), pages 507, XP002955076 *
KOPEN G.C. ET AL.: "Marrow stromal cells migrate throughout forebrain and cerebellum and they differentiate into astrocytes after injection into neonatal mouse brains", PROC. NATL. ACAD. SCI. USA, vol. 96, no. 19, 14 September 1999 (1999-09-14), pages 10711 - 10716, XP002162100 *
ROSADA C. ET AL.: "Establishment and characterization of an immortalized pluripotent telomerase-positive human bone marrow stromal cell line", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 15, no. SUPPL. 1, September 2000 (2000-09-01), pages S509, XP002955077 *
SANCHEZ-RAMOS J. ET AL.: "Adult bone marrow stromal cells differentiate into neural cells in vitro", EXP. NEUROLOGY, vol. 164, 1999, pages 247 - 256, XP002199139 *
TAN M.A. ET AL.: "Extended life span of human bone marrow endothelial cells transfected with human telomerase reverse transcriptase", BLOOD, vol. 92, no. 10, SUPPL. 1, PT. 1, 1998, pages 704A, XP002955078 *
WOODBURY D. ET AL.: "Adult rat and human bone marrow stromal cells differentiate into neurons", J. NEUROSCI. RES., vol. 61, no. 4, 15 August 2000 (2000-08-15), pages 364 - 370, XP002940452 *
WOODBURY D. ET AL.: "Adult rat and human bone marrow stromal stem cells differentiate into neurons", BLOOD CELLS, MOLECULES AND DISEASES, vol. 27, no. 3, May 2001 (2001-05-01) - June 2001 (2001-06-01), pages 632 - 636, XP002955075 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7098027B2 (en) 2000-06-26 2006-08-29 Renomedix Institute, Inc. Method for remyelinating a demyelinized lesion due to injury in the brain or spinal cord
US9115344B2 (en) 2000-06-26 2015-08-25 Nc Medical Research Inc. Mesenchymal stem cell and a method of use thereof
US10640749B2 (en) 2000-06-26 2020-05-05 Nc Medical Research Inc. Mesenchymal stem cell and the method of use thereof
US10071144B2 (en) 2013-02-06 2018-09-11 Nc Medical Research Inc. Cell therapy for the treatment of neurodegeneration
US10149894B2 (en) 2013-02-06 2018-12-11 Nc Medical Research Inc. Cell therapy for the treatment of neurodegeneration
US10874723B2 (en) 2013-02-06 2020-12-29 Nc Medical Research Inc. Cell therapy for the treatment of neurodegeneration

Also Published As

Publication number Publication date
WO2003038074A1 (fr) 2003-05-08
JP4118236B2 (ja) 2008-07-16
JPWO2003038075A1 (ja) 2005-02-24
CN1610738A (zh) 2005-04-27
KR101022349B1 (ko) 2011-03-22
KR20040062610A (ko) 2004-07-07

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