CN1607205A - Method for extracting sasanguasaponin by biofermentation - Google Patents
Method for extracting sasanguasaponin by biofermentation Download PDFInfo
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- CN1607205A CN1607205A CN 200310107891 CN200310107891A CN1607205A CN 1607205 A CN1607205 A CN 1607205A CN 200310107891 CN200310107891 CN 200310107891 CN 200310107891 A CN200310107891 A CN 200310107891A CN 1607205 A CN1607205 A CN 1607205A
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Abstract
A method for extracting oiltea saponin is characteristic of making fermentation medium from oiltea cake crude aqueous extract added with other batching, put aspergillus niger and yeast HT-6 in fermentation tank, keeping temp at 26-32 degree centigrade, tank pressure 15-60 kpa. agitating speed at 90-140 r/pm, ventililatory capacity 1:0.1-0.4, fermentation for 24-48 hours, then unloading, separating hypha and fermentation solution by plate-and-frame filter press, through decolouring fermentation solution, concentration, spraying drying to obtain said products. The cost can be reduced by 40-50 %.
Description
Technical field:
The present invention relates to a kind of extracting method of sasanguasaponin, particularly relate to a kind of method of extracting sasanguasaponin from cake of camellia oleifera seeds with microbial fermentation.
Background technology:
Oil tea is the distinctive woody oleiferous plants crop of China, because tea oil is a kind of ideal edible oil, therefore, China greatly develops this oil crops.The comprehensive utilization value of oil tea is very high, through just containing natural radioactivity composition-sasanguasaponin of about 10%～15% in the cake of camellia oleifera seeds behind the system oil.Sasanguasaponin is that it has certain physiologically active and surfactivity, and has surfactivitys such as foaming, decontamination, emulsification, dispersion, solubilising, infiltration by the complex compound of several triterpenoid saponin units and sugar chain form.Therefore, sasanguasaponin has been widely used in industrial circles such as daily use chemicals, food, textile materials.At present, development research and the comprehensive utilization for sasanguasaponin caused the great attention of China and many countries.The extracting method of sasanguasaponin mainly contains following several method at present:
1. aqueous fusion method: be to be extraction agent with water, this technology is the twentieth century fifties of method the earliest, and its main drawback is that technical process is longer, and energy consumption is bigger, and productive rate is low, and product colour is dark, and purity is not high, filters difficulty especially during actual production.
2. organic solvent method: mainly be to be extraction agent with the organic solvent, the more water-soluble method height of this handicraft product yield, quality is also better, but its cost high technology complexity.Because with an organic solvent higher to fire prevention, the requirement of explosion proof of production environment, the aftertreatment environmental pollution of organic solvent is bigger, organic solvent residue will directly influence the quality of product.
3. water extraction and alcohol precipitation method: this processing requirement is an extraction agent with water mainly, the extracting solution ethanol sedimentation after the filtration, and this technology makes quality product better, lighter color, but yield is low, and cost is higher, and the aftertreatment environmental pollution of organic solvent is bigger.
4. aqueous ethanol extraction: this technology is extraction agent with about 70% aqueous ethanolic solution mainly, and it is more that this technology leaches impurity, the aftertreatment difficulty.
Summary of the invention:
The objective of the invention is in order to overcome the problem that above-mentioned various extracting method exists, and then a kind of product yield and quality of improving is provided, reduce production costs, the microbial fermentation that reduces environmental pollution in the production process extracts the method for sasanguasaponin.
Technical solution of the present invention is as follows: 1. microbial fermentation extracts the method for sasanguasaponin, it is characterized in that may further comprise the steps: (1) is pulverized the back water logging with cake of camellia oleifera seeds and is obtained extracting solution after filtration; (2) in the 2L triangular flask, put into substratum, again aspergillus niger and yeast HT-6 are inserted respectively in the bottle, under 24～28 ℃, cultivated on 160～220 rev/mins the shaking table 20～28 hours, obtain shaking bottle and plant nutrient solution; (3) pour the extracting solution of step (1) into fermentor tank, and add the substratum material in following ratio: sal epsom 0.02～0.06%, potassium primary phosphate 0.08～0.12%, ammonium nitrate 0.5～1.0% is at 1.05kg/cm
2Down sterilization 15～30 minutes of vapor pressure, and the bottle that shakes of step (2) planted a nutrient solution and insert a fermentor tank, (4) fermentation: keep 26～32 ℃ of jar temperature, tank pressure 15～60kpa, 90～140 rev/mins of stirring velocitys, air flow 1: 0.1～0.4, fermented incubation time 24～48 hours, put jar then, separate mycelia and fermented liquid through plate-and-frame filter press.(5) with after the fermented liquid decolouring processing, through concentrating, spray, be drying to obtain the sasanguasaponin product.
Said extracting solution is that cake of camellia oleifera seeds is crushed to 40～60 orders with pulverizer, adds powder amount triple water in container, stirs and is warming up to 90 ℃, keeps 1～2 hour, obtains extracting solution through the plate-and-frame filter press coarse filtration.The said substratum proportioning of putting in the 2L triangular flask is as follows: glucose 1.3～1.6%; Sal epsom 0.02～0.06%; Potassium primary phosphate 0.08～0.12%; Yeast extract paste 0.2～0.24%; Peptone 0.42～0.55%; VB120ppm; Water 1L.It is to add 5～6% hydrogen peroxide and 1～1.2% decoloring assistant MMT-71 in the fermented liquid after separation that said decolouring is handled, and heats to 60～80 ℃, leaves standstill 2～6 hours.
The method of extraction sasanguasaponin of the present invention, the sasanguasaponin product extraction rate can reach 14～15%, and sasanguasaponin content is 35～36%; Production cost descends 40% than water extraction production cost, descends about 50% than organic solvent method production cost.In addition, the present invention does not pollute environment in process of production and fire protection requirement is low.Therefore extract sasanguasaponin with microbial fermentation pure natural property and the assurance product activity of protecting sasanguasaponin all is better than additive method.
The invention will be further described by the following examples
Embodiment 1, aspergillus niger and yeast HT-6 inserted in 6 2L triangular flasks (in the shake-flask culture base is housed) respectively and under 25 ℃, cultivates 22 hours on 200 rev/mins the shaking table; Take by weighing cake of camellia oleifera seeds 50kg, cake of camellia oleifera seeds is crushed to 60 orders with pulverizer, drop in the extractor, add 150L water and stir, and be warming up to 90 ℃, extracted 1 hour,, keep extracting solution with the plate-and-frame filter press coarse filtration.Extracting solution is put into the 300L fermentor tank, and add sal epsom 0.04%; Potassium primary phosphate 0.08%; Ammonium nitrate 0.6% is at 1.05kg/cm
2Down sterilization 30 minutes of vapor pressure; 6 2L are shaken bottle to plant in the access fermentor tank, transfer pH value to 6, keep 28 ℃ of jar temperature, tank pressure 30kpa, 120 rev/mins of stirring velocitys, air flow 1: 0.3, fermented incubation time 32 hours, put jar then, separate mycelia and fermented liquid, the hydrogen peroxide of adding 5% and 1.2% decoloring assistant MMT-71 in fermented liquid through plate-and-frame filter press, heat to 80 ℃, after leaving standstill 4 hours, concentrate the back spraying drying through the triple effect thickner, get final product 7.15kg sasanguasaponin product. must sasanguasaponin content be 35.23% after testing.
Embodiment 2: aspergillus niger and yeast HT-6 are inserted in 10 2L triangular flasks (in the shake-flask culture base is housed) respectively, under 25 ℃, cultivated 24 hours on 200 rev/mins the shaking table; Take by weighing cake of camellia oleifera seeds 50kg, cake of camellia oleifera seeds is crushed to 60 orders with pulverizer, drop in the extractor, adding 150L water stirs and is warming up to 90 ℃, extracts 2 hours, with the plate-and-frame filter press coarse filtration, keeps extracting solution.Extracting solution is put into the 300L fermentor tank and added sal epsom 0.05%; Potassium primary phosphate 0.1%; Ammonium nitrate 0.8% is at 1.05%kg/cm
2Down sterilization 30 minutes of vapor pressure; Again 10 2L are shaken bottle and plant in the access fermentor tank, transfer pH value to 6.5, keep 28 ℃ of jar temperature, tank pressure 30kpa, stirring velocity is 120 rev/mins, air flow 1: 0.3; Fermented incubation time 28 hours, put jar then, separate mycelia and fermented liquid through plate-and-frame filter press, the hydrogen peroxide of adding 6% and 1% decoloring assistant MMT-71 in fermented liquid, heat to 80 ℃, after leaving standstill 3 hours, concentrate the back spraying drying through the triple effect thickner, get final product 8.25kg sasanguasaponin product. must sasanguasaponin content be 36.14% after testing.
Claims (4)
1, microbial fermentation extracts the method for sasanguasaponin, it is characterized in that may further comprise the steps:
(1) cake of camellia oleifera seeds is pulverized the back water logging, obtain extracting solution through filtering; (2) in the 2L triangular flask, put into substratum, again aspergillus niger and yeast HT-6 are inserted respectively in the bottle, under 24～28 ℃, cultivated on 160～220 rev/mins the shaking table 20～28 hours, obtain shaking bottle and plant nutrient solution; (3) pour the extracting solution of step (1) into fermentor tank, and add the substratum material in following ratio: sal epsom 0.02～0.06%, potassium primary phosphate 0.08～0.12%, ammonium nitrate 0.5～1.0% is at 1.05kg/cm
2Down sterilization 15～30 minutes of vapor pressure, and the bottle that shakes of step (2) planted a nutrient solution and inserts a fermentor tank; (4) fermentation: keep 26～32 ℃ of jar temperature, tank pressure 15～60kpa, 90～140 rev/mins of stirring velocitys, air flow 1:0.1～0.4, fermented incubation time 24～48 hours is put jar then, separates mycelia and fermented liquid through plate-and-frame filter press; (5) the fermented liquid decolouring is handled after concentrate, spray, be drying to obtain the sasanguasaponin product.
2, according to the method for claim 1, it is characterized in that: cake of camellia oleifera seeds is crushed to 40～60 orders with pulverizer, adds powder amount triple water in container, stirs and is warming up to 90 ℃, keeps 1～2 hour, obtains extracting solution through the plate-and-frame filter press coarse filtration.
3, according to the method for claim 1, it is characterized in that: the substratum proportioning of putting in the 2L triangular flask is as follows: glucose 1.3～1.6%; Sal epsom 0.02～0.06%; Potassium primary phosphate 0.08～0.12%; Yeast extract paste 0.2～0.24%; Peptone 0.42～0.55%; VB
120ppm; Water 1L.
4, according to the method for claim 1, it is characterized in that: it is to add 5～6% hydrogen peroxide and 1～1.2% decoloring assistant MMT-71 in the fermented liquid after separation that said decolouring is handled, and heats to 60～80 ℃, leaves standstill 2～6 hours.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101880307A (en) * | 2010-06-17 | 2010-11-10 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN101906296A (en) * | 2010-05-25 | 2010-12-08 | 青田中野天然植物科技有限公司 | Camellia saponin type oilfield foaming agent and preparation method and application thereof |
| CN102154427A (en) * | 2010-11-23 | 2011-08-17 | 安徽大别山科技开发有限公司 | Method for extracting tea saponin by microbial fermentation |
| CN103276040A (en) * | 2013-05-23 | 2013-09-04 | 安徽省华银茶油有限公司 | Method for extracting tea saponin through fermentation |
| CN104357527A (en) * | 2014-10-10 | 2015-02-18 | 上海海洋大学 | Method for extracting tea saponin from tea seed meal with microbial fermentation method |
| CN104800110A (en) * | 2015-02-23 | 2015-07-29 | 福建师范大学泉港石化研究院 | Method for preparing total sapindus saponins through microbial fermentation |
| CN110664709A (en) * | 2019-11-13 | 2020-01-10 | 宜兴高等职业技术学校 | Natural shampoo |
| CN111500371A (en) * | 2020-05-12 | 2020-08-07 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
| CN112205424A (en) * | 2020-10-10 | 2021-01-12 | 江西应用技术职业学院 | Camellia oleifera cake cypress microbial metabolite and preparation method thereof |
-
2003
- 2003-10-14 CN CN 200310107891 patent/CN1607205A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101906296A (en) * | 2010-05-25 | 2010-12-08 | 青田中野天然植物科技有限公司 | Camellia saponin type oilfield foaming agent and preparation method and application thereof |
| CN101906296B (en) * | 2010-05-25 | 2013-04-24 | 青田中野天然植物科技有限公司 | Camellia saponin type oilfield foaming agent and preparation method and application thereof |
| CN101880307A (en) * | 2010-06-17 | 2010-11-10 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN101880307B (en) * | 2010-06-17 | 2013-04-03 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN102154427A (en) * | 2010-11-23 | 2011-08-17 | 安徽大别山科技开发有限公司 | Method for extracting tea saponin by microbial fermentation |
| CN102154427B (en) * | 2010-11-23 | 2013-03-27 | 安徽大别山科技开发有限公司 | Method for extracting tea saponin by microbial fermentation |
| CN103276040A (en) * | 2013-05-23 | 2013-09-04 | 安徽省华银茶油有限公司 | Method for extracting tea saponin through fermentation |
| CN104357527A (en) * | 2014-10-10 | 2015-02-18 | 上海海洋大学 | Method for extracting tea saponin from tea seed meal with microbial fermentation method |
| CN104357527B (en) * | 2014-10-10 | 2018-03-30 | 上海海洋大学 | A kind of method that microbe fermentation method extracts Tea Saponin from leached tea oil slag |
| CN104800110A (en) * | 2015-02-23 | 2015-07-29 | 福建师范大学泉港石化研究院 | Method for preparing total sapindus saponins through microbial fermentation |
| CN104800110B (en) * | 2015-02-23 | 2017-12-26 | 福建师范大学泉港石化研究院 | A kind of method that microbial fermentation prepares total saponin of sapindusmukerossi |
| CN110664709A (en) * | 2019-11-13 | 2020-01-10 | 宜兴高等职业技术学校 | Natural shampoo |
| CN111500371A (en) * | 2020-05-12 | 2020-08-07 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
| CN111500371B (en) * | 2020-05-12 | 2022-05-24 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
| CN112205424A (en) * | 2020-10-10 | 2021-01-12 | 江西应用技术职业学院 | Camellia oleifera cake cypress microbial metabolite and preparation method thereof |
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