CN1586198A - Cnidiadin and its built composition for preventing and controlling crop powdery mildew - Google Patents
Cnidiadin and its built composition for preventing and controlling crop powdery mildew Download PDFInfo
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- CN1586198A CN1586198A CN 200410069127 CN200410069127A CN1586198A CN 1586198 A CN1586198 A CN 1586198A CN 200410069127 CN200410069127 CN 200410069127 CN 200410069127 A CN200410069127 A CN 200410069127A CN 1586198 A CN1586198 A CN 1586198A
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- osthole
- powdery mildew
- propiconazole
- triazolone
- tebuconazole
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Abstract
The present invention provides the application of cnidiadin in preventing and controlling powdery mildew of crop and agricultural chemical composition for preventing and controlling powdery mildew of crop. The agricultural chemical composition consists of cnidiadin and one kind of germicide for inhibiting the synthesis of fungal ergosterol and selected from myclobutanil, triazolone, tebuconazole and propiconazol in the weight ratio of 1 to 1-20, preferably 1 to 1-10. It is especially suitable for preventing and controlling powdery mildew of cucumber, pumpkin, strawberry horse bean and grape.
Description
Technical field:
The present invention relates to agent of Osthole list and complex composition thereof, particularly itself and a kind of synthetic bactericide of fungi ergosterol that suppresses are used to prevent and treat crop powdery mildew as the rationally composite back of closing of nitrile bacterium azoles, triazolone, Tebuconazole or propiconazole as disinfectant use in agriculture.
Background technology
Coumarin kind compound Osthole (osthol) chemical name is 8-(3-methyl-2-butene base) methyl ether San shape ketone, it is Main Ingredients and Appearance in the umbellate form flower section plant frutus cnidii, also be root, the rhizome of Imperatoria ostruthium, circle Radix Angelicae Sinensis, Angelica pubescens, bolt wing celery, the active component that extracts in the acrial part of the anistree Chinese wampee root skin of rutaceae, single leaf rue etc.Medically have anticancer, antimutagenesis, killing pests and suppressing bacteria report has been arranged on agricultural, but bactericide compoundedly having a synergistic effect and compound proportion report as yet to its fungicidal spectrum with other.
For example, the inventor's the CN01127083.7 of patent formerly " application of Osthole on agricultural " discloses the application of Osthole at aspects such as pesticide-germicide, preserving fruit and vegetable utilizing and agricultural and animal products are anticorrosion, but aspect control crop germ, useful effect to eliminating vegetable botrytis bacterium, garlic tikka bacterium, the withered bacterium of asparagus stem, black rot of pear bacterium, the withered bacterium of apple leaf is only disclosed, then not open to it, particularly not open to control, the inhibitory action of various crop powdery mildew bacterium at the preventive and therapeutic effect aspect other crop germ.
Another formerly disclosed Chinese patent application CN1342412, denomination of invention relates to common cnidium fruit P.E (mainly comprising Osthole) for " application of common cnidium fruit P.E on agricultural insecticide is produced " and combine with other agricultural insecticide and is used for preventive and therapeutic effect disease worm, but these application only limit to desinsection, do not relate to the application of this composition aspect sterilization, more do not mention it in the application of crop powdery mildew bacterium aspect preventing and treating.
Powdery mildew is meant the worldwide plant disease that generally takes place that is caused by Ascomycotina gang pyrenomycetes Erysiphales Erysiphaceae (Erysiphales Erysiphaceae).Melon and fruit, wheat class, flowers, forest are lost in 58～6,500,000,000 dollars owing to what powdery mildew caused every year.In its body, form various haustoriums (a kind of form of mycelia) behind the mycelia invasion host plant and draw the nutrient of crop, make the crop morbidity, form a large amount of conidiums simultaneously crop is infected once more.The chemical control of Different Crop powdery mildew can be used identical bactericide, and for example, nitrile bacterium azoles is used to prevent and treat wheat powdery mildew, equally also is used to prevent and treat grape powdery mildew and powdery mildew of strawberry etc.
Summary of the invention
The present invention is on the basis of above-mentioned existing patented technology, by further agent of Osthole list and complex composition thereof being finished in the research, the particularly application study that prevents and treats at crop powdery mildew of agriculture field application.Its purpose be to provide be exclusively used in the control crop powdery mildew the agent of Osthole list and with other bactericide compounded composition.
Therefore, one aspect of the present invention provides the application of Osthole on the control crop powdery mildew.
The present invention provides the composition pesticide that is exclusively used in the control crop powdery mildew on the other hand, it comprises (A) Osthole and (B) a kind of synthetic bactericide of fungi ergosterol that suppresses, as be selected from the composition of one of nitrile bacterium azoles, triazolone, Tebuconazole or four kinds of compositions of propiconazole, and the weight ratio of two kinds of compositions is A/B=1: 1～1: 20, more preferably be to be 1: 1～1: 10.
The present invention is specially adapted to prevent and treat cucumber, pumpkin, strawberry, broad bean and grape powdery mildew.
Detailed description of the Invention
The agent of Osthole list has fungistatic effect preferably for various crop powdery mildews; be equivalent to the protective fungicide mancozeb such as its inhibition effect that exsomatizes; live body is because it is to the plant induced effect; therefore its preventive effect is higher than mancozeb, and its white powder bacteria-treating effective dose is at 40～100 μ g/mL.
In addition, the agent of Osthole list has better indoor activity to various crop white powder germ, makes preparation simultaneously, as reaching between 70～90% at field control effect behind microemulsion, water-based agent, the wetting powder.
Osthole and other suppress the synthetic a kind of bactericide of fungi ergosterol, a kind of as in nitrile bacterium azoles, triazolone, Tebuconazole or the propiconazole, and compounded combination has following clear superiority:
1, shows as synergy, this is because nitrile bacterium azoles, triazolone, Tebuconazole or propiconazole are to suppress the fungi ergosterol to synthesize, thereby be slow comparatively speaking, and Osthole shows as the quick-acting of directly killing to white powder germ mycelia and spore, go back inducing plant simultaneously and produce disease resistance, therefore, the two composite performance synergy;
2, composite back is compared with single agent and has been reduced the Osthole consumption, reduces the effective dependence to plant resources.And because toxicity (the rat acute per os toxicity LD of nitrile bacterium azoles
50♂=1600mg/kg, ♀=2290mg/kg; Rabbit acute dermal toxicity LD
50♂＞5000mg/kg, toxicity (mouse and the rat acute per os toxicity LD of ♀＞5000mg/kg), triazolone
50About 1000mg/kg; Rat acute is through skin LD
50＞5000mg/kg), toxicity (the rat acute per os toxicity LD of Tebuconazole
50=4000mg/kg, chmice acute per os toxicity ♂=2000mg/kg, ♀=2290mg/kg; Rat acute is through skin LD
50＞5000mg/kg), toxicity (the rat acute per os toxicity LD of propiconazole
50About 1517mg/kg, chmice acute per os toxicity LD
50♂=783mg/kg, ♀=509mg/kg; Rat acute is through skin LD
50＞4000mg/kg) all be higher than toxicity (the rat acute per os toxicity LD of Osthole
50♂＞5000mg/Kg, ♀＞5000mg/Kg; Rat acute percutaneous toxicity LD
50♂＞5000mg/Kg, ♀＞5000mg/Kg) has reduced the consumption of chemical bactericide after composite, thereby has reduced medicament toxicity.Therefore reduce behind its consumption safer to crop.
3, can administer the pesticide resistance of fungi ergosterol synthetic inhibitor such as triazolone the white powder germ after composite, consumption is reduced in the field simultaneously, thereby has reduced the selection pressure of white powder germ to this class medicament.It is reported that the powdery mildew of cucumber bacterium is to the highest having reached more than 60% of pesticide resistance of triazolone, and triazolone and nitrile bacterium azoles, Tebuconazole, propiconazole are positive cross resistance medicaments.
Osthole used in the present invention can be with known Osthole extraction process, and prior art as previously described obtains.And nitrile bacterium azoles, triazolone, Tebuconazole and the propiconazole composite with it all are commercially available Pesticidal products.Its acquisition approach is unimportant, as long as it meets ratio range of the present invention and application conditions.
To be elaborated to Osthole and with the proportion with synergistic effect of other agriculture chemical compounding composition and controlling object and composite object lesson below; these contents just are used for explanation; and do not mean that protection scope of the present invention limited that wherein said active ingredient is all in its active ingredient.
Osthole and otherSuppress the synthetic bactericide compounded indoor prescription screening of fungi ergosterol
For examination germ and host crop
With powdery mildew of cucumber bacterium (Phaerotheca fuliginea) serves as for the examination bacterium, and cucumber " No. 1, Su Chen " is for supplying the examination host crop.
Reagent agent
Reagent agent is that 94.7% Osthole (academy of agricultural sciences, Jiangsu Province provides) is dissolved in 95% ethanol, and it is standby to make 10 000 μ g/ml mother liquors; 95% nitrile bacterium azoles, 95% triazolone, 95% Tebuconazole, 93% propiconazole (Co., Ltd provides available from Jiangyin City, Jiangsu Province Fuda farmingization) are dissolved in acetone respectively, and it is standby to make 10000 μ g/ml mother liquors.
The complex composition proportion design
Virulence curve (giving birth to the survey method as described later) by preliminary experiment and single agent of being measured carries out proportion design, carries out compositely according to the compound proportion of table 1, and each composite dose all is dissolved in acetone, and it is standby to make 10000 μ g/ml mother liquors:
Table 1 Osthole and other bactericide compounded proportions and composite illustration
| Composite former medicine A: B | Compound proportion scope A: B | Composite illustration | ||
| Ratio 1 | Ratio 2 | Ratio 3 | ||
| 94.7% Osthole/95% nitrile bacterium azoles | ????1∶1～1∶20 | ????1/2 | ????1/1 | ????1/3 |
| 94.7% Osthole/95% triazolone | ????1/8 | ????1/6 | ????1/4 | |
| 94.7% Osthole/95% Tebuconazole | ????1/10 | ????1/7 | ????1/4 | |
| 94.7% Osthole/93% propiconazole | ????1/7 | ????1/5 | ????1/3 | |
Annotate: listed ratio is all calculated by medicament active ingredient in the table
It is standby then above-mentioned former medicine and composite dose to be diluted to the series concentration soup by table 2.
Each medicine series diluted concentration gradient of table 2
| Medicament | Diluted concentration gradient (ug/ml) | ||||
| 94.7% Osthole | ????200 | ????100 | ????50 | ????25 | ??12.5 |
| 95% nitrile bacterium azoles | ????50 | ????25 | ????12.5 | ????6.25 | ??3.125 |
| 95% triazolone | ????40 | ????20 | ????10 | ????5 | ??2.5 |
| 95% Tebuconazole | ????10 | ????5 | ????2.5 | ????1.25 | ??0.625 |
| 93% propiconazole | ????20 | ????10 | ????5 | ????2.5 | ??1.25 |
| 94.7% Osthole/95% nitrile bacterium azoles | ????50 | ????25 | ????12.5 | ????6.25 | ??3.125 |
| 94.7% Osthole/95% triazolone | ????20 | ????10 | ????5 | ????2.5 | ??1.25 |
| 94.7% Osthole/95% Tebuconazole | ????10 | ????5 | ????2.5 | ????1.25 | ??0.625 |
| 94.7% Osthole/93% propiconazole | ????20 | ????10 | ????5 | ????2.5 | ??1.25 |
Establishing clear water in addition is that blank is measured the susceptibility to the powdery mildew of cucumber bacterium.
Bacterium liquid is prepared:
The white powder germ that to gather from the field scrapes, and is made into conidial suspension with sterile water, and concentration is each visual field 10-20 conidium of microscopically at 10 * 10 times.
Condition determination and standard
The soup of even spraying different disposal concentration on fresh cucumber leaves, behind the 24h on every blade drop 3 points, every some 5ul spore liquid.Record morbidity progression calculates disease and refers to and preventive effect behind the 4d.
Disease refers to investigate grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Computational methods
Calculate preventive effect according to formula, utilize the percentage and the probability value table of comparisons to convert preventive effect to probit value, simultaneously dose concentration is converted into logarithm value, obtains virulence formula Y=a+bx, calculate concentration EC in correlation coefficient r and the inhibition with dosage logarithm value (x) and probit value (y)
50
The interaction of calculating mixed agent according to the method for Wadley, the effect that is mixed with the analysis of coefficient of synergism SR value:
Control efficiency (%)=[(the contrast disease refers to-handle that disease refers to)/contrast disease refers to] * 100%,
EC
50 (theoretical values)=(c+d)/[c/EC
50 (C)+ d/EC
50 (D)]
SR=EC
50 (theoretical values)/ EC
50 (real values)
C, D represent two kinds of components in the mixture respectively
C, d represent the ratio of two kinds of single agent in mixture respectively
EC
50 (real values)Practical measurement value for specific mixture)
Has synergistic effect after SR 〉=two kinds of single agent of 1.5 expressions are mixed
Has antagonism after SR≤two kinds of single agent of 0.5 expression are mixed
0.5 have summation action after≤SR≤two kinds of single agent of 1.5 expressions are mixed.
Result and analysis
Osthole and suppress the synthetic bactericide of fungi ergosterol and composite dose of variable concentrations sees Table 3,4 to the control efficiency of powdery mildew of cucumber bacterium.They are to the virulence regression equation y=a+bx of powdery mildew of cucumber bacterium, and correlation coefficient r reaches concentration EC in the effectively inhibition
50(ug/mL) and the coefficient of synergism SR of mixture see Table 5.
As can be seen from Table 5, suppress the synthetic bactericide of fungi ergosterol and under condition of living body, the specific activity Osthole of powdery mildew of cucumber bacterium is wanted high; Its composite back coefficient of synergism has synergistic effect preferably all greater than 1.5.In addition, though these triazole bactericidal agents is active higher, but the field is prone to problems such as poisoning and pesticide resistance, and Osthole also has activity preferably to the powdery mildew of cucumber bacterium under condition of living body, and this two classes medicament mechanism of action difference, there is not mutual pesticide resistance problem, can be used for alleviating the resistance problem of this type of systemic fungicide, prolong the service life of medicament so this two classes medicament is mixed.
The single agent of five kinds of sterilizations of table 3 is to the stripped control efficiency of powdery mildew of cucumber bacterium
| 95% nitrile bacterium azoles | Concentration of treatment (μ g/mL) | ??50 | ??25 | ??12.5 | ??6.25 | ??3.12 | Contrast (0) |
| Disease refers to * | ??6.3 | ??13.2 | ??16.2 | ??26.2 | ??29 | ??58.9 | |
| Preventive effect (%) | ??89 | ??78 | ??72 | ??56 | ??51 | ??/ | |
| 95% triazolone | Concentration of treatment (μ g/mL) | ??40 | ??20 | ??10 | ??5 | ??2.5 | Contrast (0) |
| Disease refers to * | ??58.9 | ??14.4 | ??17.9 | ??30.1 | ??34.4 | ??58.9 | |
| Preventive effect (%) | ??85.6 | ??75.6 | ??69.6 | ??48.9 | ??41.6 | ??/ | |
| 95% Tebuconazole | Concentration of treatment (μ g/mL) | ??10 | ??5 | ??2.5 | ??1.25 | ??0.625 | Contrast (0) |
| Disease refers to * | ??7 | ??12.9 | ??15.3 | ??25.1 | ??27.8 | ??58.9 | |
| Preventive effect (%) | ??8.1 | ??78.1 | ??74 | ??57.4 | ??52.7 | ??/ | |
| 93% propiconazole | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??5.1 | ??11.1 | ??14.9 | ??21.4 | ??26.9 | ??58.9 | |
| Preventive effect (%) | ??91.3 | ??81.2 | ??74.7 | ??63.7 | ??54.3 | ??/ | |
| Osthole | Concentration of treatment (μ g/mL) | ??50 | ??25 | ??12.5 | ??6.25 | ??3.12 | Contrast (0) |
| Disease refers to * | ??12.3 | ??15.3 | ??21 | ??26.3 | ??34 | ??58.9 | |
| Preventive effect (%) | ??79 | ??74 | ??64 | ??55 | ??42 | ??/ |
*Be the average of 4 repetitions
The different compound proportions of bactericide that table 4 Osthole is synthetic with suppressing the fungi ergosterol
To the stripped control efficiency of cucumber powdery mildew fungus
| 94.7% Osthole: 95% triazolone=1: 6 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??7 | ??11.2 | ??14.2 | ??22.2 | ??27 | ??58.9 | |
| Preventive effect *(％) | ??88.9 | ??81 | ??75.9 | ??62.3 | ??54.2 | ??/ | |
| 94.7% Osthole: 95% triazolone=1: 8 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??6 | ??9.2 | ??12.7 | ??20.2 | ??25.7 | ??58.9 | |
| Preventive effect *(％) | ??89.8 | ??84.4 | ??78.4 | ??65.7 | ??56.4 | ??/ | |
| 94.7% Osthole: 95% triazolone=1: 4 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??7.6 | ??12.7 | ??16.1 | ??24.3 | ??29.8 | ??58.9 | |
| Preventive effect *(％) | ??87.1 | ??78.4 | ??72.7 | ??58.7 | ??50.6 | ??/ | |
| 94.7% Osthole: 95% Tebuconazole=1: 10 | Concentration of treatment (μ g/mL) | ??10 | ??5 | ??2.5 | ??1.25 | ??0.625 | Contrast (0) |
| Disease refers to * | ??5.3 | ??9.1 | ??12.3 | ??18.9 | ??23.3 | ??58.9 | |
| Preventive effect *(％) | ??91 | ??84.6 | ??79.1 | ??67.9 | ??60.4 | ??/ | |
| 94.7% Osthole: 95% Tebuconazole=1: 7 | Concentration of treatment (μ g/mL) | ??10 | ??5 | ??2.5 | ??1.25 | ??0.625 | Contrast (0) |
| Disease refers to * | ??5.9 | ??10.2 | ??13.7 | ??20.3 | ??26.1 | ??58.9 | |
| Preventive effect *(％) | ??90 | ??82.7 | ??76.7 | ??65.5 | ??55.7 | ??/ | |
| 94.7% Osthole: 95% Tebuconazole=1: 4 | Concentration of treatment (μ g/mL) | ??10 | ??5 | ??2.5 | ??1.25 | ??0.625 | Contrast (0) |
| Disease refers to * | ??6.3 | ??11.6 | ??15.4 | ??20.3 | ??29.2 | ??58.9 | |
| Preventive effect *(％) | ??89.3 | ??80.3 | ??73.9 | ??65.5 | ??50.4 | ??/ | |
| 94.7% Osthole: 95% nitrile bacterium azoles=1: 1 | Concentration of treatment (μ g/mL) | ??50 | ??25 | ??12.5 | ??6.25 | ??3.125 | Contrast (0) |
| Disease refers to * | ??6.7 | ??10.3 | ??14.1 | ??20.1 | ??27.9 | ??58.9 | |
| Preventive effect *(％) | ??88.6 | ??82.5 | ??76.1 | ??65.9 | ??52.8 | ??/ | |
| 94.7% Osthole: 95% nitrile bacterium azoles=1: 2 | Concentration of treatment (μ g/mL) | ??50 | ??25 | ??12.5 | ??6.25 | ??3.125 | Contrast (0) |
| Disease refers to * | ??5.4 | ??9.5 | ??12.9 | ??19.3 | ??25.1 | ??58.9 | |
| Preventive effect *(％) | ??90.8 | ??83.9 | ??78.1 | ??67.2 | ??57.4 | ??/ |
| 94.7% Osthole: 95% nitrile bacterium azoles=1: 3 | Concentration of treatment (μ g/mL) | ??50 | ??25 | ??12.5 | ??6.25 | ??3.125 | Contrast (0) |
| Disease refers to * | ??4.9 | ??8.4 | ??11.7 | ??17.9 | ??23.1 | ??58.9 | |
| Preventive effect *(％) | ??91.7 | ??85.7 | ??80.1 | ??69.6 | ??60.8 | ??/ | |
| 94.7% Osthole: 93% propiconazole=1: 7 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??5.1 | ??9.1 | ??12.5 | ??18.8 | ??23.7 | ??58.9 | |
| Preventive effect *(％) | ??91.3 | ??84.6 | ??78.8 | ??68.1 | ??60.8 | ??/ | |
| 94.7% Osthole: 93% propiconazole=1: 5 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??5.9 | ??10.7 | ??13.6 | ??20.1 | ??25.2 | ??58.9 | |
| Preventive effect *(％) | ??90 | ??81.8 | ??76.9 | ??65.8 | ??57.2 | ??/ | |
| 94.7% Osthole: 93% propiconazole=1: 3 | Concentration of treatment (μ g/mL) | ??20 | ??10 | ??5 | ??2.5 | ??1.25 | Contrast (0) |
| Disease refers to * | ??6.7 | ??11.8 | ??14.7 | ??21.3 | ??27.7 | ??58.9 | |
| Preventive effect *(％) | ??88.6 | ??80 | ??70.6 | ??58.1 | ??50.9 | ??/ |
*Be the mean value of 4 repetitions
The composite indoor prescription The selection result of table 5 Osthole and triazole bactericidal agent
| Medicament | Virulence regression equation | Correlation coefficient r | Actual 50% effective concentration E C50(μg/ml) | Theoretical 50% effective concentration E C50??(μg/ml) | Coefficient of synergism SR |
| 94.7% Osthole | y＝18.6767+32x | ??0.97 | ??108.6 | ??/ | ??/ |
| 95% triazolone | y＝26.45+13.14x | ??0.988 | ??8.456 | ??/ | ??/ |
| 94.7% Osthole: 95% triazolone=1: 8 | y＝24.457+16.6x | ??0.968 | ??5.608 | ??9.42 | ??1.68 |
| 94.7% Osthole: 95% triazolone=1: 6 | y＝29.371+17.24x | ??0.996 | ??4.058 | ??9.739 | ??2.4 |
| 94.7% Osthole: 95% triazolone=1: 4 | y＝23.34+17.23x | ??0.975 | ??5.457 | ??10.368 | ??1.9 |
| 95% Tebuconazole | y＝19.35+23.24x | ??0.978 | ??7.96 | ??/ | ??/ |
| 94.7% Osthole: 95% Tebuconazole=1: 10 | y＝18.65+33.16x | ??0.97 | ??4.551 | ??8.692 | ??1.91 |
| 94.7% Osthole: 95% Tebuconazole=1: 7 | y＝20.9+11.5x | ??0.99 | ??3.864 | ??9 | ??2.33 |
| 94.7% Osthole: 95% Tebuconazole=1: 4 | y＝17.94+8.3x | ??0.959 | ??5.552 | ??9.771 | ??1.76 |
| 95% nitrile bacterium azoles | y＝31.26+11.33x | ??0.964 | ??11.36 | ??/ | ??/ |
| 94.7% Osthole: 95% nitrile bacterium azoles=1: 1 | y＝17.22+7.659x | ??0.972 | ??18.42 | ??28.18 | ??1.53 |
| 94.7% Osthole: 95% nitrile bacterium azoles=1: 2 | y＝15.49+20.13x | ??0.98 | ??14.06 | ??25.3 | ??1.8 |
| 94.7% Osthole: 95% nitrile bacterium azoles 1: 3 | y＝19.56+23.56x | ??0.966 | ??11.82 | ??20.57 | ??1.74 |
| 93% propiconazole | y＝16.584+27.4x | ??0.986 | ??7.132 | ??/ | ??/ |
| 94.7% Osthole: 93% propiconazole=1: 7 | y＝16.74+15.26x | ??0.979 | ??4.562 | ??8.075 | ??1.77 |
| 94.7% Osthole: 93% propiconazole=1: 5 | y＝16.371+7.25x | ??0.976 | ??3.505 | ??8.447 | ??2.41 |
| 94.7% Osthole: 93% propiconazole=1: 3 | y＝27.924+8.73x | ??0.985 | ??4.797 | ??9.306 | ??1.94 |
Osthole and other bactericide compounded preparation field control effectiveness test
" pesticide field efficacy medicine test criterion " by the chief editor of The Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC designs the field control effectiveness test of complex preparation to various crop powdery mildews.
Experimental scheme
Concrete experimental scheme sees Table 6:
Table 6 field control effectiveness test medicament
| For studying thing | Controlling object | Test medicine | The contrast medicament |
| Cucumber variety is excellent No. 4 of Tianjin | Powdery mildew of cucumber bacterium (E.cucurbitacearum) | 1% Osthole EW | 50% tmtd WP |
| 20% triazolone EC | |||
| 5% Osthole triazolone SE | |||
| 7% Osthole triazolone SE | |||
| 9% Osthole triazolone SE | |||
| Grape variety is huge rich | Grape powdery mildew bacterium (P.pyri) | 1% Osthole EW | The high WG of 10% generation |
| 6% Tebuconazole ME | |||
| 5% Osthole Tebuconazole SE | |||
| 8% Osthole Tebuconazole SE | |||
| 11% Osthole Tebuconazole SE | |||
| Broad bean kind Nantong rascal | Broad bean white powder germ (P.pisi) | 1% Osthole EW | 40% lucky star EC |
| 12.5% nitrile bacterium azoles WP | |||
| 12% Osthole nitrile bacterium azoles SE | |||
| 16% Osthole nitrile bacterium azoles SE | |||
| 10% Osthole nitrile bacterium azoles SE | |||
| Strawberry cultivars is Feng Xiang | Powdery mildew of strawberry bacterium (S.rosae) | 1% Osthole EW | The high WG of 10% generation |
| 25% propiconazole EC | |||
| 4% Osthole propiconazole SE | |||
| 6% Osthole propiconazole SE | |||
| 8% Osthole propiconazole SE | |||
| Pumpkin variety is that east rises | Squash marble dust germ (E.cucurbitacearum) | 1% Osthole EW | 15% triazolone W |
| 25% propiconazole EC | |||
| 4% Osthole propiconazole SE | |||
| 6% Osthole propiconazole SE | |||
| 8% Osthole propiconazole SE |
Test medicine:
1% Osthole EW250 *, the Jiangsu Province Agriculture Science Institute, valid density is 40ug/ml.
12.5% nitrile bacterium azoles WP1500 *, Zhangjagang City seven continent pesticide chemical Co., Ltds produce, and valid density is 83.3ug/ml.
2% Osthole nitrile bacterium azoles SE500 * (active ingredient: 1% Osthole+1% nitrile bacterium azoles), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+20ug/ml nitrile bacterium azoles.
3% Osthole nitrile bacterium azoles SE500 * (active ingredient: 1% Osthole+2% nitrile bacterium azoles), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+40ug/ml nitrile bacterium azoles.
4% Osthole nitrile bacterium azoles SE500 * (active ingredient: 1% Osthole+3% nitrile bacterium azoles), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+60ug/ml nitrile bacterium azoles.
25% propiconazole EC1200 *, Changzhou bumper harvest insecticide factory produces, and valid density is 208.3ug/ml.
4% Osthole propiconazole SE500 * (active ingredient: 1% Osthole+3% propiconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+60ug/ml propiconazole.
6% Osthole propiconazole SE500 * (active ingredient: 1% Osthole+5% propiconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+100ug/ml propiconazole.
8% Osthole propiconazole SE500 * (active ingredient: 1% Osthole+7% propiconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+140ug/ml propiconazole.
6% Tebuconazole ME250 *, Changzhou bumper harvest insecticide factory produces, and valid density is 240ug/ml.
5% Osthole Tebuconazole SE500 * (active ingredient: 1% Osthole+4% Tebuconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+80ug/ml Tebuconazole.
8% Osthole Tebuconazole SE500 * (active ingredient: 1% Osthole+7% Tebuconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+140ug/ml Tebuconazole.
11% Osthole Tebuconazole SE500 * (active ingredient: 1% Osthole+10% Tebuconazole), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+200ug/ml Tebuconazole.
20% triazolone EC1000 * (Changzhou bumper harvest insecticide factory produces), valid density is 200ug/ml
5% Osthole triazolone SE500 * and (active ingredient: 1% Osthole+4% triazolone) Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+80ug/ml triazolone.
7% Osthole triazolone SE500 * (active ingredient: 1% Osthole+6% triazolone), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+120ug/ml triazolone.
9% Osthole triazolone SE500 * (active ingredient: 1% Osthole+8% triazolone), the Jiangsu Province Agriculture Science Institute, valid density is 20ug/ml Osthole+160ug/ml triazolone.
The contrast medicament:
50% tmtd WP500 *, emerging orchard worker pharmaceutical factory, Tianjin produces, and valid density is 1000ug/ml.
The high WG1000 of 10% generation *, Shanghai just reaches earlier (China) Investment Co., Ltd and produces, and valid density is 100 μ g/ml.
15% triazolone WP800 *, east, Yangzhou precious pesticide chemical Co., Ltd produces, and valid density is 187.5 μ g/ml.
40% lucky star EC9000 *, Jiangsu Jiannong Agrochemical Co., Ltd. produces, and valid density is 44.4 μ g/ml blanks: clear water.
Wherein, SE represents the water suspension emulsion, and EW represents aqueous emulsion, and WP represents wetting powder, and WG represents water-soluble granule.
The sub-district is provided with
According to the result of preliminary experiment the series concentration gradient is set and carries out small plot experiment, 20 square metres of every sub-districts, every processing repeats for 4 times.In the disease phase dispenser of starting, test is with the artificial knapsack sprayer dispenser of workers and peasants-16 type, to squirt blade tow sides soup following droplet degree of being not just, 50 kilograms/mu of usefulness amount of liquid medicine.
Disease survey:
Investigation state of an illness radix before the 1st dispenser, preventive effect behind investigation medicine last time before each dispenser, investigation in 10 days behind the last medicine, i.e. 5 samplings in each sub-district, every some investigation 3 strains are during the investigation fruit, 3～5 of every strain investigation, during the investigation blade, get three leaf investigation in every strain upper, middle and lower, the percentage that accounts for the whole blade area with lesion area carries out classification.
Grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole blade area;
3 grades: lesion area accounts for 6%～15% of whole leaf area;
5 grades: lesion area accounts for 16%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
The drug effect computational methods:
Control efficiency (%)=(1-CK
0* Pt
1/ CK
1* Pt
0) * 100% (CK
0, CK
1Respectively before the representative contrast medicine, disease refers to behind the medicine, Pt
0, Pt
1Before representing the treatment region medicine respectively, disease refers to behind the medicine)
Other influence to crop
Whether have poisoning produce, have poisoning then to write down the poisoning degree if observing:
-: no poisoning;
+: slight poisoning, plant growth there is not influence;
++: obviously poisoning, can restore, can not cause crop failure;
The poisoning of +++: height influences the crop normal growth, and crop yield and quality are caused to a certain degree loss;
++ ++: serious poisoning, plant growth is obstructed, and output and mass loss are serious.
Result of the test
Each medicament is to the crop powdery mildew field control effect
Each medicament sees Table 7 to the crop powdery mildew field control effect, the every 667m of control broad bean powdery mildew
2With 1% Osthole EW250 *, 12.5% nitrile bacterium azoles WP1500 *, 2% Osthole nitrile bacterium azoles SE500 *, 3% Osthole nitrile bacterium azoles SE500 *, 4% Osthole nitrile bacterium azoles SE500 * wait the average preventive effect of medicament to be respectively 65.4%, 75.3%, 88.6%, 87.4%, 89.3%, contrast medicament 40% lucky star EC9000 * average preventive effect be 84.7%.The results of analysis of variance shows, handle medicament 2% Osthole nitrile bacterium azoles SE500 *, 3% Osthole nitrile bacterium azoles SE500 *, 4% Osthole nitrile bacterium azoles SE500 * with contrast medicament 50% tmtd WP500 * preventive effect between do not have significant difference, and and two single agent 1% Osthole EW250 *, 12.5% nitrile bacterium azoles WP1500 * preventive effect between difference reach utmost point significance level.
Prevent and treat the every 667m of powdery mildew of cucumber
2With 1% Osthole EW250 *, 20% triazolone EC1000 *, 5% Osthole triazolone SE500 *, 7% Osthole triazolone SE500 *, 9% Osthole triazolone SE500 * wait the average preventive effect of medicament to be respectively 67.7%, 71.8%, 84.5%, 81.9%, 88.8%, contrast medicament 50% tmtd WP500 * preventive effect be 85.3%.The results of analysis of variance shows, handle medicament 5% Osthole triazolone SE500 *, 7% Osthole triazolone SE500 *, 9% Osthole triazolone SE500 * with contrast medicament 50% tmtd WP500 * preventive effect between do not have significant difference, and and two single agent 1% Osthole EW250 *, 20% triazolone EC1000 * preventive effect between difference reach utmost point significance level.
The every 667m of control grape powdery mildew
2With 1% Osthole EW250 *, 6% Tebuconazole ME250 *, 5% Osthole Tebuconazole SE500 *, 8% Osthole Tebuconazole SE500 *, 11% Osthole Tebuconazole SE500 * wait the average preventive effect of medicament to be respectively 67.3%, 75.9%, 87.3%, 89.3%, 87.9%, contrast medicament 10% generation high WG1000 * preventive effect be 88.3%.The results of analysis of variance shows, 5% Osthole Tebuconazole SE500 *, 8% Osthole Tebuconazole SE500 *, 11% Osthole Tebuconazole SE500 * preventive effect and contrast medicament 10% generation high WG1000 * preventive effect between do not have significant difference, and two single agent 1% Osthole EW250 *, 6% Tebuconazole ME250 * preventive effect between difference reach utmost point significance level.
The every 667m of control powdery mildew of strawberry
2With 1% Osthole EW250 *, 25% propiconazole EC1200 *, 4% Osthole propiconazole SE500 *, 6% Osthole propiconazole SE500 *, 8% Osthole propiconazole SE500 * wait the average preventive effect of medicament to be respectively 70%, 71.25%, 78.2%, 80.2%, 84.7%, contrast medicament 10% generation high WG1000 * preventive effect be 76.7%.The results of analysis of variance shows, 4% Osthole propiconazole SE500 *, 6% Osthole propiconazole SE500 *, 8% Osthole propiconazole SE500 * preventive effect and single agent 1% Osthole EW250 * preventive effect and 25% propiconazole EC1200 * preventive effect between utmost point significant difference; 4% Osthole propiconazole SE500 * preventive effect and contrast medicament 10% generation high WG1000 * preventive effect between do not have significant difference; 6% Osthole propiconazole SE500 *, 8% Osthole propiconazole SE500 * preventive effect and contrast medicament 10% generation high WG1000 * preventive effect between significant difference.
The every 667m of control squash marble dust
2With 1% Osthole EW250 *, 25% propiconazole EC1200 *, 4% Osthole propiconazole SE500 *, 6% Osthole propiconazole SE500 *, 8% Osthole propiconazole SE500 * wait the average preventive effect of medicament to be respectively 65.7%, 80.3%, 85.7%, 84.1%, 85.2%, contrast medicament 15% triazolone WP800 * preventive effect be 72.3%.The results of analysis of variance shows, 4% Osthole propiconazole SE500 *, 6% Osthole propiconazole SE500 *, 8% Osthole propiconazole SE500 * preventive effect and contrast medicament 15% triazolone WP800 * preventive effect and single agent 1% Osthole EW250 * preventive effect between reach utmost point significant difference, and 25% propiconazole EC1200 * preventive effect between significant difference.
Evaluating drug
Each composite dose all has the better prevention effect to the crop powdery mildew, and preventive effect can reach about 80%, is higher than the preventive effect that respectively contrasts single agent, can effectively prevent and treat the harm of white powder germ to various crops.
Observe according to system behind the medicine, duration of test does not see that poisoning takes place, and each test medicine has no adverse effects to plant growth.
Table 7 Osthole and triazole bactericidal agent complex preparation are prevented and treated various crop powdery mildew field control effectiveness test results
| Handle medicament | Controlling object | Disease index before the medicine * | Behind the medicine 7 days for the third time | |||
| Disease refers to * | Disease refers to growth rate *(％) | Preventive effect *(％) | Poisoning | |||
| 1% Osthole EW250 * | The broad bean powdery mildew | ??9.7 | ??15.6 | ??60.8 | ??65.4Cc | ??- |
| 12.5% nitrile bacterium azoles WP1500 * | ??8.8 | ??10.1 | ??14.8 | ??75.3Bb | ??- | |
| 2% Osthole nitrile bacterium azoles SE500 * | ??9.1 | ??4.8 | ??-47.3 | ??88.6Aa | ??- | |
| 3% Osthole nitrile bacterium azoles SE500 * | ??9.5 | ??5.6 | ??-41 | ??87.4Aa | ??- | |
| 4% Osthole nitrile bacterium azoles SE500 * | ??8.7 | ??4.3 | ??-50.6 | ??89.3Aa | ??- | |
| 40% lucky star EC9000 * | ??9.3 | ??6.6 | ??-29 | ??84.7Aab | ??- | |
| The clear water contrast | ??9.4 | ??43.7 | ??364.9 | ??/ | ??- | |
| 1% Osthole EW250 * | Powdery mildew of cucumber | ??10.5 | ??17.96 | ??71 | ??67.7Cc | ??- |
| 20% triazolone EC1000 * | ??9.9 | ??14.8 | ??49.5 | ??71.8Bbc | ??- | |
| 5% Osthole triazolone SE500 * | ??10.3 | ??8.5 | ??-17.5 | ??84.5Aa | ??- | |
| 7% Osthole triazolone SE500 * | ??8.5 | ??8.1 | ??-4.7 | ??81.9Aab | ??- | |
| 9% Osthole triazolone SE500 * | ??7.3 | ??4.3 | ??41.1 | ??88.8Aa | ??- | |
| 50% tmtd WP500 * | ??8.1 | ??6.3 | ??-22.2 | ??85.3Aa | ??- | |
| The clear water contrast | ??9.5 | ??50.3 | ??429.5 | ??/ | ??- | |
| 1% Osthole EW250 * | Grape powdery mildew | ??7.9 | ??11.3 | ??43 | ??67.3Cc | ??- |
| 6% Tebuconazole ME250 * | ??7.9 | ??8.3 | ??5.1 | ??75.9Bb | ??- | |
| 5% Osthole Tebuconazole SE500 * | ??8.6 | ??17.4.89 | ??-44.2 | ??87.3Aa | ??- | |
| 8% Osthole Tebuconazole SE500 * | ??9.6 | ??18.84.5 | ??-53.1 | ??89.3Aa | ??- | |
| 11% Osthole Tebuconazole SE500 * | ??8.2 | ??14.23.5 | ??-57.3 | ??87.9Aa | ??- | |
| The high WG1000 of 10% generation * | ??9.1 | ??4.6 | ??-49.5 | ??88.3Aa | ??- | |
| The clear water contrast | ??7.4 | ??32.3 | ??336.5 | ??/ | ??- | |
| 1% Osthole EW250 * | Powdery mildew of strawberry | ??10.3 | ??15.6 | ??51.46 | ??70.Bbc | ??- |
| 25% propiconazole EC1200 * | ??9.7 | ??14.1 | ??45.36 | ??71.25Bbc | ??- | |
| 4% Osthole propiconazole SE500 * | ??9.6 | ??10.6 | ??10.42 | ??78.2Ab | ??- | |
| 6% Osthole propiconazole SE500 * | ??8.9 | ??8.9 | ??0 | ??80.2Aab | ??- | |
| 8% Osthole propiconazole SE500 * | ??10.1 | ??7.8 | ??-22.77 | ??84.7Aab | ??- | |
| The high WG1000 of 10% generation * | ??9.5 | ??11.2 | ??17.89 | ??76.7Ab | ??- | |
| The clear water contrast | ??10.6 | ??53.6 | ??405.66 | ??/ | ??- | |
| 1% Osthole EW250 * | Squash marble dust | ??6.3 | ??15.7 | ??149.2 | ??65.7Cc | ??- |
| 25% propiconazole EC1200 * | ??6.1 | ??8.7 | ??42.6 | ??80.3Ab | ??- | |
| 4% Osthole propiconazole SE500 * | ??6.5 | ??6.8 | ??4.6 | ??85.7Aa | ??- | |
| 6% Osthole propiconazole SE500 * | ??6.79 | ??7.9 | ??16.3 | ??84.1Aa | ??- | |
| 8% Osthole propiconazole SE500 * | ??6.5 | ??7 | ??7.7 | ??85.2Aa | ??- | |
| 15% triazolone WP800 * | ????5.9 | ????11.9 | ????101.7 | ????72.3Bb | - | |
| The clear water contrast | ????5.2 | ????37.8 | ????626.9 | ????/ | - |
*Be the mean value of 4 repetitions
Safety testing
In other bactericide of above-mentioned and fructus cnidii complex ratio, its consumption is below single agent conventional amount used half or half, therefore it is satisfactory to people and animals' toxicity with safety to crop, and in above-mentioned prescription, the content of Osthole is the highest 1%, through assert that the toxicity test result of mechanism shows that 1% osthole water-emulsion agent belongs to the micro-virus kind agricultural chemicals:
Rat acute per os toxicity LD50 ♂＞5000mg/Kg, ♀＞5000mg/Kg;
Rat acute percutaneous toxicity LD50 ♂＞5000mg/Kg, ♀＞5000mg/Kg;
It is 0 (1h) that lagophthalmos stimulates integration, is 0 (1: 100) dilution behind the 48h, to the eye nonirritant;
It is 0 (4h) that lagophthalmos stimulates integration, to the skin nonirritant.
Embodiment (Osthole and other bactericide compounded example):
Embodiment 1:2% Osthole nitrile bacterium azoles SE500 * (active ingredient: 1% Osthole+1% nitrile bacterium azoles, valid density is 20 μ g/ml Ostholes+20 μ g/ml nitrile bacterium azoles) medication at the initial stage of a disease of control grape powdery mildew, use 100ml for every mu, later stage, every mu of dosage reached 200～300ml along with blade increases
Embodiment 2:4% Osthole nitrile bacterium azoles SE500 * (active ingredient: 1% Osthole+3% nitrile bacterium azoles, valid density is 20 μ g/ml Ostholes+60 μ g/ml nitrile bacterium azoles) medication at the initial stage of a disease of control broad bean powdery mildew, use 100ml for every mu, later stage, every mu of dosage reached 200～300ml along with blade increases.
Embodiment 3:8% Osthole Tebuconazole SE500 * (active ingredient: 1% Osthole+7% Tebuconazole, valid density is that 20 μ g/ml Ostholes+140 μ g/ml Tebuconazoles control cucumber, pumpkin, custard squash, muskmelon, powdery mildew of strawberry are at the initial stage of a disease with 500 times of sprayings, use 100ml for every mu, medication was 1 time in per 5～7 days.Later stage, every mu of dosage reached 200～300ml along with blade increases.
Embodiment 4:7% Osthole triazolone SE500 * (active ingredient: 1% Osthole+6% nitrile bacterium azoles, valid density are 20 μ g/ml Ostholes+120 μ g/ml triazolones.The control grape powdery mildew begins spraying at the initial stage of a disease, uses 100ml for every mu, spray in per 5～7 days 1 time.Later stage, every mu of dosage reached 200～300ml along with blade increases.
Embodiment 5:8% Osthole propiconazole SE500 * (active ingredient: 1% Osthole+7% nitrile bacterium azoles, valid density are 20 μ g/ml Ostholes+140 μ g/ml triazolones.Control strawberry, squash marble dust begin spraying at the initial stage of a disease, use 100ml for every mu, spray in per 5～7 days 1 time.Later stage, every mu of dosage reached 200～300ml along with blade increases.
Claims (6)
1. the application of Osthole on the control crop powdery mildew.
2. application as claimed in claim 1, wherein said crop are cucumber, pumpkin, strawberry, broad bean and grape.
3. be exclusively used in the composition pesticide of control crop powdery mildew, it comprises that (A) Osthole suppresses the synthetic bactericide of fungi ergosterol with (B) a kind of, and the weight ratio of two kinds of compositions is A/B=1: 1～1: 20.
4. composition pesticide as claimed in claim 3, the synthetic bactericide of wherein said inhibition fungi ergosterol is the composition that is selected from one of nitrile bacterium azoles, triazolone, Tebuconazole or four kinds of compositions of propiconazole
5. composition pesticide as claimed in claim 3, wherein the weight ratio of A and B composition is for being 1: 1～1: 10.
6. as any one described composition pesticide among the claim 3-5, be used to prevent and treat cucumber, pumpkin, strawberry, broad bean and grape powdery mildew.
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