CN1563346A - Method for cultivating micro alga and photo-biologic reactor system utilized - Google Patents
Method for cultivating micro alga and photo-biologic reactor system utilized Download PDFInfo
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- CN1563346A CN1563346A CN 200410017249 CN200410017249A CN1563346A CN 1563346 A CN1563346 A CN 1563346A CN 200410017249 CN200410017249 CN 200410017249 CN 200410017249 A CN200410017249 A CN 200410017249A CN 1563346 A CN1563346 A CN 1563346A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
Abstract
A multistage closed type of photobiological reactor system is integrated by multipiece photobiological reactor in closed type. the method for cultivating microalgae includes adding culture medium in the first stage closed photobiological reactor (CPR), cultivating culture solution at each stage CPR, transfering culture solution from last stage CPR to next stage CPR and collecting product at tail stage CPR.
Description
Technical field
The present invention relates to a kind of bioreactor and cultivate the method for little algae and the photo-bioreactor system of use.
Background technology
Existing little algae is cultivated and mainly carries out in bioreactor, has succeeded in developing bioreactor of a great variety, that structure is different so far, and broad varietys such as open pond formula, horizontal tubular, vertical tubular, flexible bag-like are arranged.Representational have two classes: runway pond formula and closed photo bioreactor.The former be suitable for can open culture little algae, production cost is lower, and can keep certain output in big area is produced, and is a large amount of generally culture systems of employing of little algae institute of cultivating of present commercial size.And closed photo bioreactor can be used for the cultivation of most of algae, is easy to control culture condition and growth parameter(s); CO
2The utilization ratio height.Level circle round cast and vertical tubular are wherein more typically arranged, be applicable to unsuitable open culture or the higher algae of economic worth, can prevent and remedy pollution, keep single plant cultivation, the strict control of operation process energy, the higher efficiency of light energy utilization be arranged, thereby can obtain higher biological density and output.The culturing process of the present little algae of closed photo bioreactor mainly may further comprise the steps: 1. clean bioreactor, sterilization, inoculation; 2. in closed photo bioreactor, add substratum; 3. in bioreactor, inflate, carry out the inflation of nutrient solution and cultivate; 4. collect little algae product; 5. add new substratum.Above-mentioned culturing process, each circulation all will once clean, sterilizes and inoculate and just can carry out new next time cultivation circulation, the quantity of cultivating is less relatively, the efficiency ratio of cultivating is lower, generally relatively be suitable for the laboratory and use, be used for the cost that industrialization is produced has then correspondingly increased cultivation; This best cultivation, the interpolation of substratum is finished by manual, wastes time and energy, and uses water pump to add the temperature variation that substratum then causes the pollution of substratum easily and causes because of the running of water pump; And if the employing part is collected the mode that cultured products is also partly added substratum simultaneously, because monomeric bioreactor is cultivated in relatively independent mode, on the time that is collected in of the interpolation of substratum and cultured products and the space is consecutive, some is the substratum that just adds to cause the cultured products of collecting easily, can influence the effect of cultivating, cause the inefficiency of cultivation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method of little algae and photo-bioreactor system of use cultivated at above-mentioned prior art present situation, be used for a large amount of, quick, economic, the cultivation efficiently of little algae, at short notice can cultured continuously so that a large amount of highdensity little algaes to be provided, and can continue quite long period, be applicable to the needs of little algae cultivation industrialization.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of method of cultivating little algae, in a multistage photo-bioreactor system, to carry out, and it may further comprise the steps: 1. add substratum in first step closed photo bioreactor; 2. carrying out the inflation of nutrient solution in each grade closed photo bioreactor cultivates, 3. the nutrient solution in the upper level closed photo bioreactor is transported in the next stage closed photo bioreactor, 4. from last one-level closed photo bioreactor, collects little algae product.
1. described step is to finish in the mode of gravity flow by a high-order water container and a filling thrust-augmenting nozzle that connects described high-order water container and described first step closed photo bioreactor that accommodates substratum.
4. described step is to finish in the mode of gravity flow by the monomeric closed photo bioreactor of a low level water container and a described last one-level of connection and the collection thrust-augmenting nozzle of described low level water container.
3. described step is to finish by the connection thrust-augmenting nozzle that connects adjacent closed photo bioreactor.
A kind of photo-bioreactor system that is used to cultivate little algae, the multistage closed photo bioreactor system that forms by the closed photo bioreactor more than two, interconnect by connecting thrust-augmenting nozzle between the described closed photo bioreactor, a high-order water container links to each other with first step closed photo bioreactor by the filling thrust-augmenting nozzle, described high-order water container is carried substratum by described filling thrust-augmenting nozzle to described first step closed photo bioreactor, a low level water container links to each other with described final stage closed photo bioreactor by collecting thrust-augmenting nozzle, and described low level water container is collected little algae product by collecting thrust-augmenting nozzle from described final stage closed photo bioreactor.
Described a kind of photo-bioreactor system, the Pyatyi closed photo bioreactor of forming by five closed photo bioreactors preferably, interconnect by connecting thrust-augmenting nozzle between the described closed photo bioreactor, described high-order water container links to each other with first step closed photo bioreactor by the filling thrust-augmenting nozzle, described high-order water container is carried substratum by described filling thrust-augmenting nozzle to described first step closed photo bioreactor, described low level water container links to each other with described level V closed photo bioreactor by collecting thrust-augmenting nozzle, and described low level water container is collected little algae product by collecting thrust-augmenting nozzle from described level V closed photo bioreactor.
Described connection thrust-augmenting nozzle is provided with the glass threeway.
The bottom of the urceolus of described closed photo bioreactor is conical structure.
The opening of described connection thrust-augmenting nozzle is arranged between the urceolus and inner core of described closed photo bioreactor.
Compared with prior art, the culturing process that the invention has the advantages that little algae is to carry out in a multistage photo-bioreactor system of being made up of high-order water container, low level water container and a plurality of closed photo bioreactor, the collection of the interpolation of substratum, the cultivation of nutrient solution and cultured products is in a continuum of states, thereby can carry out a large amount of cultivations of little algae continuously, rapidly, efficiently, and keep longer cultivation period; The interpolation of substratum and cultured products be collected in relative isolation on the time and space, keep culture condition in the bioreactor to be more suitable for the growth of culture, collecting has higher effect, makes culture systems can bring into play function for a long time, effectively; A high-order water container is set, by the filling thrust-augmenting nozzle substratum is wherein flowed automatically in the bioreactor by syphonic effect, the bioreactor cultured products flows automatically to a low level water container by collecting thrust-augmenting nozzle, can save the man power and material, avoid the pollution of substratum simultaneously and because of the energy dissipation that running caused of water pump and the caused temperature variation of running of water pump; Connect with a glass threeway in the middle of connecting thrust-augmenting nozzle, gas in the purger easily, regulate the liquid level height of adjacent closed photo bioreactor, help the interpolation and the derivation of nutrient solution, and can add nutritive salt or other nutrient solution at any time and regulate material, reach the purpose of fast culture; The opening of connection thrust-augmenting nozzle that connects adjacent closed photo bioreactor is between the inner/outer tube of bioreactor, consistent with the nutrient solution round-robin direction in the closed photo bioreactor, can not expose liquid level, in whole culturing process, can both stably be positioned on the position of setting.
Cultural method of the present invention, the growth of cultivating little algae can keep long exponential phase of growth, and gather in the crops continuously, lag phase, the stage of stable development have been reduced than traditional method, it is rapider to grow, and the conventional culture process of culturing process ratio has reduced cleaning, sterilization, the inoculation of bioreactor each time, and the lag phase of micro algae growth, exponential phase of growth, the stage of stable development and senescence phase, have higher economic benefit, and be suitable for large-scale industrialization production.
As the Pyatyi photo-bioreactor system that adopts five bioreactors to connect into, can carry out a large amount of fast culture of all common little algaes, add fresh culture with 3～6 liters/hour speed, collecting cultured products with same speed, is 8～30 times of the density of first bioreactor in the density of a last bioreactor.The speed of growth of culture and density are common 2～5 times, and the cycle of cultivation is common 1/2～1/5.
Description of drawings
Fig. 1 is a Pyatyi closed photo bioreactor system schematic of the present invention;
The Pyatyi closed photo bioreactor system schematic that Fig. 2 overlooks for the present invention;
Fig. 3 is the structural representation (omitting high and low position water container) of the embodiment of the invention four;
Fig. 4 is the synoptic diagram of one of closed photo bioreactor structure of the present invention;
Fig. 5 is two synoptic diagram of closed photo bioreactor structure of the present invention;
Fig. 6 is one of test-results of the embodiment of the invention one and the cultivation results synopsis of prior art;
Fig. 7 be the embodiment of the invention one test-results two with the cultivation results synopsis of prior art.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
Embodiment one: a kind of method of cultivating little algae, in a Pyatyi photo-bioreactor system 1, carry out, may further comprise the steps: 1. in first step closed photo bioreactor 11, add substratum in the mode of gravity flow by a high-order water container 4 and a filling thrust-augmenting nozzle 5 that connects high-order water container 4 and first step closed photo bioreactor 11 that accommodates substratum; 2. in each grade closed photo bioreactor, charge into CO by gas-filled valve 9
2Gas also carries out the circulation cultivation of nutrient solution, 3. the nutrient solution in the closed photo bioreactor of upper level is transported in the closed photo bioreactor of next stage by the connection thrust-augmenting nozzle 3 that connects adjacent closed photo bioreactor, 4. from level V closed photo bioreactor 15, collects little algae product in the mode of gravity flow by a low level water container 6 and a collection thrust-augmenting nozzle 7 that connects level V closed photo bioreactor 15 and low level water container 6.
In a Pyatyi photo-bioreactor system, carried out following culture experiment:
Test with Asia heart-shaped flat algae Platymonas subcordiformis high-density culture is an example, adopts the XB.K25 blood counting chamber, shows in OLYMPUS-CH30 microscopically count results: cultivate 10d, frustule is by initial inoculum density 6.4 * 10
4Individual/ml; Rise to 25.2 * 10 behind the 2d
4Individual/ml; Rise to maximum 111.5 * 10 behind the 4d
4Individual/ml; Rise to 390.0 * 10 behind the 6d
4Individual/ml; Rise to 758.3 * 10 behind the 8d
4Individual/ml; Rise to 1200 * 10 behind the 10d
4Individual/ml; Put the algae liquid of 1/3rd volumes, the add-back fresh culture continues to cultivate, and cultivates 2d, algae cell density 800 * 10
4Individual/ml is elevated to 1350 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 900 * 10
4Individual/ml is elevated to 2170 * 10
4Individual/ml, put the algae liquid of 1/2nd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 1035 * 10
4Individual/ml is elevated to 1500 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 750 * 10
4Individual/ml is elevated to 1300 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 780 * 10
4Individual/ml is elevated to 1300 * 10
4Individual/ml.As shown in Figure 6, and compare, the high-density and the speed of growth that can reach all are to adopt the present invention that bigger advantage is arranged.
The high-density culture of carrying out Nitzschia closterium minutissima Nitzschia closterium with interpolation silicon is an example, and the cell density of inoculation is 60.6 * 10 when cultivating beginning
4Individual/ml, rise to 408.3 * 10 behind the 2d
4Individual/ml; Rise to 1336.7 * 10 behind the 4d
4Individual/ml; Rise to 2378.3 * 10 behind the 6d
4Individual/ml; Rise to 4483.3 * 10 behind the 8d
4Individual/ml; Put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 2990 * 10
4Individual/ml is elevated to 4800 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 3200 * 10
4Individual/ml is elevated to 5230 * 10
4Individual/ml, put the algae liquid of 1/2nd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 2620 * 10
4Individual/ml is elevated to 3800 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 2530 * 10
4Individual/ml is elevated to 4300 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 2870 * 10
4Individual/ml is elevated to 4000 * 10
4Individual/ml, put the algae liquid of 1/3rd volumes, the add-back fresh medium continues to cultivate, and cultivates 2d, algae cell density 2670 * 10
4Individual/ml is elevated to 4000 * 10
4Individual/ml, this for quick, the high-density culture food organism are significant.As shown in Figure 7, with the comparison of contrast, the high-density that the high-density culture Nitzschia closterium minutissima can reach and the speed of growth all are to adopt the bigger advantage that has of the present invention.
Embodiment two: a kind of method of cultivating little algae, a secondary light bioreactor system 1 ' in carry out, may further comprise the steps: 1. by a high-order water container 4 that accommodates substratum ' and one connect high-order water container 4 ' and first step closed photo bioreactor 11 ' filling thrust-augmenting nozzle 5 ' in the mode of gravity flow to first step closed photo bioreactor 11 ' middle interpolation substratum; 2. in each grade closed photo bioreactor by gas-filled valve 9 ' charge into CO
2Gas also carries out the circulation cultivation of nutrient solution, 3. by connect thrust-augmenting nozzle 3 ' with first step closed photo bioreactor 11 ' in nutrient solution be transported to second stage closed photo bioreactor 12 ' in, 4. by a low level water container 6 ' and one connect second stage closed photo bioreactor 12 ' and low level water container 6 ' collection thrust-augmenting nozzle 7 ' with the mode of gravity flow from second stage closed photo bioreactor 12 ' the little algae product of collection.
Embodiment three: a kind of photo-bioreactor system, the Pyatyi closed photo bioreactor system 1 that forms by five closed photo bioreactors, five closed photo bioreactors are arranged on the support 2, interconnect by connecting thrust-augmenting nozzle 3 between each closed photo bioreactor, a high-order water container 4 links to each other with first step closed photo bioreactor 11 by filling thrust-augmenting nozzle 5, high-order water container 4 is carried substratum by filling thrust-augmenting nozzle 5 to first step closed photo bioreactor 11, a low level water container 6 links to each other with level V closed photo bioreactor 15 by collecting thrust-augmenting nozzle 7, and low level water container 6 is collected cultured products by collecting thrust-augmenting nozzle 7 from level V closed photo bioreactor 15; Connect thrust-augmenting nozzle 5 and be provided with glass threeway 8, the bottom of the urceolus 101 of closed photo bioreactor preferably is conical structure or cylinder-like structure, the opening 31 that connects thrust-augmenting nozzle 3 is arranged between the urceolus 101 and inner core 102 of closed photo bioreactor, filling thrust-augmenting nozzle 5 and collection thrust-augmenting nozzle 7 can be provided with the regulated valve (not shown), bottom at the urceolus 101 of closed photo bioreactor is provided with inlet mouth 103, be connected to the inflation mechanism (not shown) by a gas-filled valve 9, the top of the inner core 102 of closed photo bioreactor is provided with air leakage hole 104, connect thrust-augmenting nozzle 3 and can connect adjacent closed photo bioreactor, can also connect adjacent closed photo bioreactor from the position of inlet mouth 103 from the position of air leakage hole 104.Inner core 102 leaves overflow weir 105 near the upper end of urceolus 101, inner core 102 leaves return port 106 near urceolus 101 lower ends, utilize the buoyancy of bubble to drive nutrient solution in the inner core 102, the upwelling that is formed on inner core 102 with the liquid circulation of the orientation of the katabatic drainage of urceolus 101, support 2 is provided with the regulatable illumination apparatus 10 that is looped around around the Pyatyi closed photo bioreactor system 1.
Embodiment four: a kind of photo-bioreactor system, by two closed photo bioreactors form two-stage closed photo bioreactor system 1 ', between each closed photo bioreactor by connecting thrust-augmenting nozzle 3 ' interconnect, high-order water container 4 ' by filling thrust-augmenting nozzle 5 ' with first step closed photo bioreactor 11 ' link to each other, the high-order water container 4 ' thrust-augmenting nozzle 5 that passes through to annotate ' to first step closed photo bioreactor 11 ' conveying substratum, low level water container 6 ' by collect thrust-augmenting nozzle 7 ' with second stage closed photo bioreactor 12 ' link to each other, low level water container 6 ' by collection thrust-augmenting nozzle 7 ' from second stage closed photo bioreactor 12 ' the collection cultured products; Connect thrust-augmenting nozzle 5 ' be provided with glass threeway 8 ', first step closed photo bioreactor 11 ' the bottom of urceolus 101 be conical structure, second stage closed photo bioreactor 12 ' the bottom of urceolus 101 be cylinder-like structure, connect thrust-augmenting nozzle 3 ' opening 31 ' be arranged between the urceolus 101 and inner core 102 of closed photo bioreactor, filling thrust-augmenting nozzle 5 ' and collection thrust-augmenting nozzle 7 ' can be provided with the regulated valve (not shown), bottom at the urceolus 101 of closed photo bioreactor is provided with inlet mouth 103, by a gas-filled valve 9 ' be connected to inflation mechanism (not shown), the top of the inner core 102 of closed photo bioreactor is provided with air leakage hole 104, connect thrust-augmenting nozzle 3 and can connect adjacent closed photo bioreactor, can also connect adjacent closed photo bioreactor from the position of inlet mouth 103 from the position of air leakage hole 104.Inner core 102 leaves overflow weir 105 near the upper end of urceolus 101, inner core 102 leaves return port 106 near urceolus 101 lower ends, utilize the buoyancy of bubble to drive nutrient solution in the inner core 102, the upwelling that is formed on inner core 102 with the liquid circulation of the orientation of the katabatic drainage of urceolus 101.
Claims (8)
1, a kind of method of cultivating little algae is characterized in that it may further comprise the steps: 1. add substratum in first step closed photo bioreactor; 2. carrying out the inflation of nutrient solution in each grade closed photo bioreactor cultivates, 3. the nutrient solution in the upper level closed photo bioreactor is transported in the next stage closed photo bioreactor, 4. from last one-level closed photo bioreactor, collects little algae product.
2, a kind of method of cultivating little algae as claimed in claim 1 is characterized in that 1. described step is to finish in the mode of gravity flow by a high-order water container and a filling thrust-augmenting nozzle that connects described high-order water container and described first step closed photo bioreactor that accommodates substratum.
3, a kind of method of cultivating little algae as claimed in claim 1 is characterized in that 4. described step is to finish in the mode of gravity flow by a low level water container and a collection thrust-augmenting nozzle that connects described last one-level closed photo bioreactor and described low level water container.
4, a kind of method of cultivating little algae as claimed in claim 1 is characterized in that 3. described step is to finish by the connection thrust-augmenting nozzle that connects adjacent closed photo bioreactor.
5, a kind of photo-bioreactor system that is used to cultivate little algae, it is characterized in that the multistage closed photo bioreactor system that it is made up of the closed photo bioreactor more than two, interconnect by connecting thrust-augmenting nozzle between the described closed photo bioreactor, a high-order water container links to each other with first step closed photo bioreactor by the filling thrust-augmenting nozzle, described high-order water container is carried substratum by described filling thrust-augmenting nozzle to described first step closed photo bioreactor, a low level water container links to each other with described final stage closed photo bioreactor by collecting thrust-augmenting nozzle, and described low level water container is collected little algae product by collecting thrust-augmenting nozzle from described final stage closed photo bioreactor.
6, a kind of photo-bioreactor system as claimed in claim 6, it is characterized in that the Pyatyi closed photo bioreactor system that it is made up of five closed photo bioreactors, interconnect by connecting thrust-augmenting nozzle between the described closed photo bioreactor, described high-order water container links to each other with first step closed photo bioreactor by the filling thrust-augmenting nozzle, described high-order water container is carried substratum by described filling thrust-augmenting nozzle to described first step closed photo bioreactor, described low level water container links to each other with described level V closed photo bioreactor by collecting thrust-augmenting nozzle, and described low level water container is collected little algae product by collecting thrust-augmenting nozzle from described level V closed photo bioreactor.
7,, it is characterized in that described connection thrust-augmenting nozzle is provided with the glass threeway as claim 6 or 7 described a kind of photo-bioreactor systems.
8, a kind of photo-bioreactor system as claimed in claim 7, the bottom that it is characterized in that the urceolus of described closed photo bioreactor is conical structure, and the opening of described connection thrust-augmenting nozzle is arranged between the urceolus and inner core of described closed photo bioreactor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410017249 CN1272429C (en) | 2004-03-26 | 2004-03-26 | Method for cultivating micro alga and photo-biologic reactor system utilized |
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| CN 200410017249 CN1272429C (en) | 2004-03-26 | 2004-03-26 | Method for cultivating micro alga and photo-biologic reactor system utilized |
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| CN1563346A true CN1563346A (en) | 2005-01-12 |
| CN1272429C CN1272429C (en) | 2006-08-30 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101914431A (en) * | 2010-07-23 | 2010-12-15 | 江苏省海洋水产研究所 | Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system |
| WO2011050578A1 (en) * | 2009-10-29 | 2011-05-05 | 新奥科技发展有限公司 | Method for culture of microalgae and photobioreactor system thereof |
| CN101781620B (en) * | 2009-01-20 | 2012-10-17 | 云南爱尔发生物技术有限公司 | Auxiliary pipeline system supporting operation of bioreactor group and operation process thereof |
| CN110468025A (en) * | 2019-09-06 | 2019-11-19 | 王习羽 | It is a kind of suitable for heterotrophism and the micro algae culturing device and cultural method of mixotrophic cultivation |
| CN111500462A (en) * | 2020-04-13 | 2020-08-07 | 青岛旭能生物工程有限责任公司 | Method for industrial culture of nannochloropsis |
| CN112359075A (en) * | 2020-10-15 | 2021-02-12 | 云南维他源生物科技有限公司 | Large-scale production method of fucoxanthin |
-
2004
- 2004-03-26 CN CN 200410017249 patent/CN1272429C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101781620B (en) * | 2009-01-20 | 2012-10-17 | 云南爱尔发生物技术有限公司 | Auxiliary pipeline system supporting operation of bioreactor group and operation process thereof |
| WO2011050578A1 (en) * | 2009-10-29 | 2011-05-05 | 新奥科技发展有限公司 | Method for culture of microalgae and photobioreactor system thereof |
| CN101914431A (en) * | 2010-07-23 | 2010-12-15 | 江苏省海洋水产研究所 | Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system |
| CN101914431B (en) * | 2010-07-23 | 2013-05-08 | 江苏省海洋水产研究所 | Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system |
| CN110468025A (en) * | 2019-09-06 | 2019-11-19 | 王习羽 | It is a kind of suitable for heterotrophism and the micro algae culturing device and cultural method of mixotrophic cultivation |
| CN111500462A (en) * | 2020-04-13 | 2020-08-07 | 青岛旭能生物工程有限责任公司 | Method for industrial culture of nannochloropsis |
| CN112359075A (en) * | 2020-10-15 | 2021-02-12 | 云南维他源生物科技有限公司 | Large-scale production method of fucoxanthin |
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| Publication number | Publication date |
|---|---|
| CN1272429C (en) | 2006-08-30 |
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