CN1552889A - Use and preparation of collagen protein and collagen polypeptide - Google Patents
Use and preparation of collagen protein and collagen polypeptide Download PDFInfo
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- CN1552889A CN1552889A CNA2003101040474A CN200310104047A CN1552889A CN 1552889 A CN1552889 A CN 1552889A CN A2003101040474 A CNA2003101040474 A CN A2003101040474A CN 200310104047 A CN200310104047 A CN 200310104047A CN 1552889 A CN1552889 A CN 1552889A
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Abstract
This invention relates to preparations of collagen proteins and collagen peptides. It comprises: 1) mechanically pulverizing alkali leather or raw leather 100, washing at atmosphere and regulating pH 7- 10; 2) adding degreasing enzyme 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 - 45 deg.C, washing; 3) adding surfactant 0.1 - 1.2, stirring for 0.5 - 4 hours at 20 -45 deg.C, washing; 4) adding active biological enzyme 0.1 - 0.8 at 30 - 65 deg.C, degrading for 0.5 - 12 hours; 5) heating to 75 - 90 deg.C for 15 - 30 minutes to inactivating enzyme; 6) filtering and distilling at 40 - 65 deg.C with pressure reduction; and 7) spraying to dry and obtain solid collagen proteins and collagen peptides powders. Owing to alkali degreasing enzyme and surfactant, leather degreasing rate will be up to 95% to produce easily soluble products with low cost and high productivity. The products can be used in skin nourishment and healthy food.
Description
Technical field
The present invention relates to a kind of method for preparing collagen protein and collagen polypeptide and uses thereof.
Background technology
Collagen protein is rich in 19 seed amino acids, 7 seed amino acids and 2 kinds of children's semi-dispensable amino acids comprising needed by human, more than the contained amino acid of people lactoprotein two kinds, its protein content is higher 4 times than milk powder, sheep milk powder, and collagen protein now has been widely used in fields such as medicine, chemical industry, food.Polypeptide is compared with amino acid with protein has more advantages, and the specific absorption of polypeptide in enteron aisle is very high, and the specific absorption of some low peptides such as dipeptides, tripeptides is faster than the amino acid of same composition.Physiological functions such as polypeptide has low antigen, promotes lipid metabolism, reducing cholesterol, promotion mineral substance absorb, hypotensive, anti-oxidant.Some polypeptide have the immunity of organism of reinforcement, promote the metabolic effect of human body.Polypeptide has acid and thermostability, water-soluble preferably, and viscosity is blunt with change in concentration.
Animal skin is the byproduct of carnivorous industry, also is the raw material of tanning industry, mainly is made up of collagen protein.Animal raw skin utilization ratio in the process hides process only is 20%-30%, and tanning waste output is huge, and China is process hides big country, will produce more than 140 ten thousand tons of skin corner wastes every year, not only causes the significant wastage of resource, and brings serious environmental to pollute.
Utilize hide offal (alkali skin) to make collagen protein at present and mainly adopt enzymolysis process.Enzymolysis process reaction conditions gentleness need not specific installation, and amino acid is not destroyed, no racemization, and have the advantages that the cycle is short, energy consumption is low, yield is high.But because singly with defatting enzyme or grease-removing agent degreasing, degreasing effect is bad, and washing time is long, the product of producing forms milk with lipid in water, can't separate, and is unfavorable for the use of collagen protein and collagen polypeptide products at present.
Summary of the invention
The purpose of this invention is to provide a kind of method for preparing collagen protein and collagen polypeptide, the collagen product that this method is produced does not contain lipid, and high density dissolving back clear helps the use of collagen product.
Another object of the present invention provides a kind of purposes of the said products.
For achieving the above object, the technical solution adopted in the present invention is: a kind of method for preparing collagen protein and collagen polypeptide, it may further comprise the steps: 1) 100 parts of alkali skins after the mechanical fragmentation or rawhide normal temperature are washed to pH value be 7～10; 2) add 0.1～1.2 part of defatting enzyme, stir degreasing 0.5～4 hour at 20～45 ℃, washing; 3) add 0.1～1.2 part of tensio-active agent, stir degreasing after 0.5～4 hour, washing at 20～45 ℃; 4) in the time of 30～65 ℃, add 0.1～0.8 part of active biological enzyme, degraded 0.5～12 hour; 5) be warming up to 75～90 ℃, be incubated 15～30 minutes, make enzyme deactivation; 6) filter, filtrate is carried out underpressure distillation in 40～65 ℃; 7) spraying drying obtains solid state collagen albumen and collagen polypeptide powder.
The tensio-active agent that degreasing is adopted in the said process is one or more in complexed surfactant and the cats product.Complexed surfactant is soil temperature series, peregal series or polyether series.Above-mentioned skimming processes is to adopt the alkaline lipase degreasing earlier, just adds tensio-active agent then.Discover: only adopt the defatting enzyme degreasing, degreasing rate is 83.1%, and washing is removed but foreign protein is difficult easily, and the solubleness of the product of producing in water is lower.As only adopting the tensio-active agent degreasing, degreasing rate is 95%, but washing time is long, and the energy consumption height is unfavorable for following process.If skimming processes adopts the method for using tensio-active agent earlier with defatting enzyme again, on the basis that does not reduce degreasing effect, can raise the efficiency greatly, reduce cost.
In addition, insulation is 15～35 minutes when being better than most 75～85 ℃ after the degreasing washing, carries out thermal destruction.Can impel collegen filament structure dispersion and loose like this, for follow-up enzymolysis process creates conditions.The used active biological enzyme of enzymolysis process comprises neutral protease, Sumizyme MP and aspartic protease.During use, can adopt one or more enzyme to carry out enzymolysis.
The molecular weight size and the distribution of collagen protein and collagen polypeptide products can be controlled by changing enzymatic hydrolysis condition.The kind of enzyme and consumption, enzymolysis time and temperature, pH value etc. when enzymatic hydrolysis condition comprises enzymolysis, wherein the pH value is regulated with corresponding bronsted lowry acids and bases bronsted lowry according to the potential of hydrogen of selected active biological enzyme, such as HCL, NaOH.
100～600 parts of deionized waters are adopted in washing described in the present invention, and washing times is 2～3 times, and distillation pressure is 0.06～0.10Mpa.
Collagen protein that the present invention is prepared and collagen polypeptide products can be used for preparing the skin-nourishing protective foods.
The invention has the beneficial effects as follows: owing to utilize alkaline lipase and tensio-active agent that the alkali skin is carried out degreasing, degreasing rate can reach 95% like this, and the collagen product of producing is soluble in water, and can raise the efficiency greatly, reduces cost.The present invention also carries out thermal destruction to the raw material after the degreasing, impels collegen filament structure tendency dispersion and loose, for follow-up enzymolysis creates conditions.
The present invention is further illustrated below by the mode of embodiment, but therefore do not limit the present invention in the described scope of embodiments.
Embodiment
Embodiment one:
100g alkali skin after the mechanical fragmentation is put in the 500ml flask, adds the 400g deionized water, stirring at normal temperature washing 2 times to pH value is about 9, and liquid anhydrates.Add 400g deionized water, 0.6g alkaline lipase again, handled 30 minutes in 40 ℃ of stirred in water bath, liquid anhydrates.Add peregal 1 gram, soil temperature-80 1 gram, stearic alcohol ether 0.5 gram and the 400g deionized water of polyoxyethylene-21 again, similarity condition continues reaction after 1 hour down, and liquid anhydrates.Add the 100g deionized water, and be warming up to 80 ℃, constant temperature was handled 30 minutes, carried out thermal destruction.Be cooled to 40 ℃ again, add 0.3g trypsinase, degraded 3 hours, transferring PH with HCL again is about 6.5, add 0.6g microorganism 1398 proteolytic enzyme, degraded 8 hours, be warming up to 90 ℃ again, be incubated 20 minutes, make enzyme deactivation, more after filtration, filtrate is carried out underpressure distillation in about 50 ℃, spray-dried at last, promptly get solid state collagen albumen and collagen polypeptide powder.
The said products is as follows with the product data contrast of producing with additive method:
| Sebum fat content | The polypeptide products solvability | Product dissolving transparency | |
| Singly use lipase | ????10.1％ | Generally | Bad |
| Product of the present invention | ????5.6％ | ????＞40％ | Clear |
Above-mentioned fat test method is with reference to GB.
Embodiment two:
100g alkali skin after the mechanical fragmentation is put in the 500ml flask, adds the 400g deionized water, stirring at normal temperature washing 2 times, to pH value be about 9, liquid anhydrates.Add 400g deionized water, 0.2g defatting enzyme again, handled 30 minutes in 40 ℃ of stirred in water bath, liquid anhydrates.Add peregal-OP 1 gram again, soil temperature-60 2 gram, stearic alcohol ether 0.5 gram and the 400g deionized water of polyoxyethylene-21, similarity condition continues reaction after 1 hour down, and liquid anhydrates.Add the 100g deionized water, and be warming up to 80 ℃, constant temperature was handled 30 minutes, carried out thermal destruction.Be cooled to 40 ℃ again, transferring PH with HCL is about 2, adds the 0.6g stomach en-, degraded 6 hours, transferring PH with NaOH again is about 6.5, adds the 0.2g neutral protease, degraded 4 hours, be warming up to 90 ℃ again, be incubated 20 minutes, make enzyme deactivation, again after filtration, filtrate is carried out underpressure distillation in about 50 ℃, spray-dried at last, promptly gets solid state collagen albumen and collagen polypeptide powder.
The product contrast that product that the inventive method is produced and additive method are produced is as follows:
| Sebum fat content | The product solvability | Product dissolving transparency | |
| Singly use lipase | 10.2％ | ～30％ | Milk sap |
| Product of the present invention | 3.3％ | ＞40％ | Clear |
Above-mentioned fat test method is with reference to GB.
Embodiment three:
The collagen protein of embodiment one and embodiment two preparations and the property testing of collagen polypeptide powder
The chromatograph (AKTA Explorer 100) that adopts Pharmacia (Pharmacia) company to produce is analyzed, and the molecular-weight average of collagen protein and collagen polypeptide is respectively about 20,000 and about 2000, and the average peptide chain length is 100～130 and 10～13.
Embodiment four
The collagen protein and the collagen polypeptide powder of embodiment one and embodiment two preparations are used for the female skin protective foods, and the weight percent of its composition comprises: collagen protein and collagen polypeptide 65%; Soybean isoflavones 5%; Soybean polypeptide 15%; Chinese yam 10% and vitamins C 5%.
Its concrete production method is: at first said components is mixed in mixing tank, adds 95% medical alcohol mixing granulation, pour out the back in baking oven in 40 ℃ of dryings 2 hours, then by the filling machine capsule of packing into No. 1.Instructions of taking: take 4 every day, take two weeks and can see good result, especially fine to skin, internal secretion and climacteric women effect.
Claims (9)
1, a kind of method for preparing collagen protein and collagen polypeptide, it is characterized in that: it may further comprise the steps:
1) 100 weight part alkali skins after the mechanical fragmentation or rawhide normal temperature are washed to pH value be 7～10;
2) add 0.1～1.2 weight part defatting enzyme, stir degreasing 0.5～4 hour at 20～45 ℃, washing;
3) add 0.1～1.2 weight part tensio-active agent, stir degreasing after 0.5～4 hour, washing at 20～45 ℃;
4) in the time of 30～65 ℃, add 0.1～0.8 weight part active biological enzyme, degraded 0.5～12 hour;
5) be warming up to 75～90 ℃, be incubated 15～30 minutes, make enzyme deactivation;
6) filter, filtrate is carried out underpressure distillation in 40～65 ℃;
7) spraying drying obtains solid state collagen albumen and collagen polypeptide powder.
2, according to the described method for preparing collagen protein and collagen polypeptide of claim 1, it is characterized in that: described tensio-active agent is at least a in complexed surfactant and the cats product.
3, according to the described method for preparing collagen protein and collagen polypeptide of claim 2, it is characterized in that: described complexed surfactant is soil temperature series, peregal series or polyether series.
4, want the 1 described method for preparing collagen protein and collagen polypeptide according to right, it is characterized in that: described defatting enzyme is an alkaline lipase.
5, according to the described method for preparing collagen protein and collagen polypeptide of claim 1, it is characterized in that: in step 2) back thermal destruction step in addition.
6, according to the described method for preparing collagen protein and collagen polypeptide of claim 1, it is characterized in that: described distillation pressure is 0.06～0.10Mpa.
7, according to the described method for preparing collagen protein and collagen polypeptide of claim 1, it is characterized in that: 100～600 parts by weight of deionized water are adopted in described washing, and washing times is 2～3 times.
8, collagen protein and the collagen polypeptide with the described method preparation of claim 1 is used to prepare the skin-nourishing protective foods.
9, according to the described skin-nourishing protective foods of claim 8, it is characterized in that: the weight percent of its composition comprises: collagen protein and collagen polypeptide 65%; Soybean isoflavones 5%; Soybean polypeptide 15%; Chinese yam 10% and vitamins C 5%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101040474A CN1552889A (en) | 2003-12-18 | 2003-12-18 | Use and preparation of collagen protein and collagen polypeptide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101040474A CN1552889A (en) | 2003-12-18 | 2003-12-18 | Use and preparation of collagen protein and collagen polypeptide |
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| CN1552889A true CN1552889A (en) | 2004-12-08 |
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| CNA2003101040474A Pending CN1552889A (en) | 2003-12-18 | 2003-12-18 | Use and preparation of collagen protein and collagen polypeptide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948898A (en) * | 2010-09-15 | 2011-01-19 | 上海东锦饮品有限公司 | Method for preparing nano oligopeptide collagen |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN103842517A (en) * | 2011-08-03 | 2014-06-04 | 莲花组织修复公司 | Collagen 7 and related methods |
| CN106509549A (en) * | 2015-09-15 | 2017-03-22 | 宁辉 | Pure collagen milk and preparation method thereof |
| CN106701878A (en) * | 2017-02-20 | 2017-05-24 | 青海师范大学 | Method for extracting yak skin collagen by adopting enzyme process |
-
2003
- 2003-12-18 CN CNA2003101040474A patent/CN1552889A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948898A (en) * | 2010-09-15 | 2011-01-19 | 上海东锦饮品有限公司 | Method for preparing nano oligopeptide collagen |
| CN101948898B (en) * | 2010-09-15 | 2013-06-26 | 日加满饮品(上海)有限公司 | Method for preparing nano oligopeptide collagen |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102154420B (en) * | 2010-11-11 | 2013-05-22 | 湖北远成药业有限公司 | Method for extracting pigskin collagen peptides |
| CN103842517A (en) * | 2011-08-03 | 2014-06-04 | 莲花组织修复公司 | Collagen 7 and related methods |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN106509549A (en) * | 2015-09-15 | 2017-03-22 | 宁辉 | Pure collagen milk and preparation method thereof |
| CN106701878A (en) * | 2017-02-20 | 2017-05-24 | 青海师范大学 | Method for extracting yak skin collagen by adopting enzyme process |
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