CN1545997A - Preparation method of sun-screening and skin care product - Google Patents
Preparation method of sun-screening and skin care product Download PDFInfo
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- CN1545997A CN1545997A CNA2003101236085A CN200310123608A CN1545997A CN 1545997 A CN1545997 A CN 1545997A CN A2003101236085 A CNA2003101236085 A CN A2003101236085A CN 200310123608 A CN200310123608 A CN 200310123608A CN 1545997 A CN1545997 A CN 1545997A
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Abstract
A process for preparing novel sun-proof skin care product which comprises, employing biological extraction, chromatography, ultrasonic and freeze drying technologies, extracting oligonucleotide molecules from sardine, charging the dried powder of oligonucleotide into the base-material of the skin care product by themass ratio of 1:1000, agitating homogeneously, loading to obtain the sun-proof skin care product.
Description
Technical field
The invention belongs to skincare product, be specifically related to a kind of novel skin care item with sun-proof function.
Background technology
According to World Health Organization's interrelated data statistics, the whole world is annual to be increased 2,000,000 to 3,000,000 skin carcinoma patient, 2,000,000 cataract patients newly and surpasses 13 extremely evil property melanoma patients.Tanning by the sun in the sun is the major reason that causes these diseases.Exposure in sunshine also may reduce people's immunologic function, thereby causes other illness.The report of World Health Organization (WHO) is also pointed out UV-induced disease is focused on prevention, and reminds people is guarded against because of tanning by the sun of solar ultraviolet and suffered from skin carcinoma and cataract.
Ultraviolet wavelength (400-100nm) is the shortest in various sunrays, and its photon energy and frequency are the highest, and it can penetrate and injure skin, and the ultraviolet of different wave length can form sterilizing area, erythema zone, solarization black area to human body skin.Ultraviolet C district (UV-C): be called as sterilizing area, comprise the light wave of wavelength from 100 to 280nm, though it can destroy the human skin tissue cell, the ultraviolet in most of this district has been filtered by atmospheric ozone layer the time, does not still have the report that this district's ultraviolet damages human body skin at present.Ultraviolet B district (UV-B): wavelength is between 280-320nm, and it is also referred to as the area of illumination of burning, be cause skin burn, indirectly pigmentation, skin tanning, erythema occurs and cause the zone of skin carcinoma.Ultraviolet A district (UV-A): wavelength is between 320-400nm, B district relatively, and it causes the probability of skin erythema less, but it is to cause skin tanning with direct pigmentation, causes skin premature aging, wrinkle and causes the zone that the skin carcinoma probability is bigger.
Currently used chemical sunscreen, composition all be organic compound (as amino benzoic Acid, Salicylate, Avobenz Benzophenone-3), can cause skin sensitivity, SPF (sun protection factor) is high more, causes the skin sensitivity chance high more.Also having and adopt reflection class sunscreen, as zinc oxide etc., is to replace organic compound with mineralogical composition in fact, still might cause the composition of skin sensitivity in the product.Though these sunlight screening skin-protecting product centering long wave ultraviolets have barrier effect, but erythema prevented, reduce melanic secretion, but these sunscreen productses can not effectively stop the intermediate waves ultraviolet to the human body gene damage; And its sun-proof result causes the excretory minimizing of melanin, can cause human body to defend the ability of short wave ultraviolet to reduce normally like this.Sun-proof market in urgent need is a kind of can effectively to absorb the ultraviolet green sunlight screening skin-protecting product that in the solar irradiation human body gene is damaged.
Summary of the invention
The technical problem that the present invention mainly solves is that the ultraviolet oligonucleotide molecules that can effectively absorb that will extract in marine organism is added on the skin care item, prepares a kind of brand-new ultraviolet sunlight screening skin-protecting product that can effectively absorb in the solar irradiation the human body gene damage.
Process for preparing novel sun-proof skin care of the present invention is as follows:
A, employing biotechnology are extracted oligonucleotide molecules in the sardine body: sand is cleaned in fish, be ground into pasty state, add the distilled water of 5 times of volumes, carry out centrifugal treating behind the stirring and evenly mixing, get the upper strata water after the centrifugal treating, boiled 8-12 minute, carry out the centrifugal treating second time after the cooling again, collect the upper strata water then, every 1000ml adds non-specific Cobra venom endonuclease 1-4mg, act on 6-9 hour down at 37 ℃, promptly get oligonucleotide chromatography sample.
Oligonucleotide chromatography sample is crossed the medium pressure chromatography post, is the gradient NaCl solution stepwise elution of 0.1-1.0mol/L with concentration, and collecting with 0.5-0.8 mol/L NaCl, pH7.4 is the eluent of eluting phase time; Eluent carries out supersound process behind the dialysis desalination, lyophilization then obtains oligonucleotide dry powder.
The compound method of B, sunlight screening skin-protecting product: pass through orthogonal experiment, determine the oligonucleotide content of adding and the proportion compatibility of skin-care product base, the mass ratio of oligonucleotide dry powder with 1: 1000 added in the base material of skin care item, the common process flow process that adopts skin care item to produce, stir, fill, promptly make the finished product of sunlight screening skin-protecting product.By experiment, prove that this compatibility relationship can reach effect effectively sun-proof and the prevention relevant disease.
The present invention has carried out the sun-proof result experiment to sunlight screening skin-protecting product:
Adopt rabbit to do the skin allergy experiment, identify the sun-proof result of this sunlight screening skin-protecting product.Get healthy rabbits respectively, establish experimental group and matched group, experimental group is smeared sunlight screening skin-protecting product shaving the hair-fields, and matched group is smeared common emollient cream, discontinuous irradiation under uviol lamp, and concurrent irradiation 10 times each 10 minutes, is observed after twice, 5 day every day.The result shows, the blister and the phenomenon of casting off a skin appear in the baring skin generation anaphylaxis of matched group, and it is normal to smear the rabbit skin of sunlight screening skin-protecting product.Biologic activity assay certificate, these goods can play good sun-proof effect, illustrate that be desirable with nucleic acid as sunscreen.
According to the golden shadow sunscreen cream that preparation method of the present invention is produced, entrust toxicity teaching and research room of HSPH of Mountain Western Medicine S University that this product has been carried out rat acute per os toxicity test and percutaneous toxicity test.
Acute oral toxicity (LD
50): behind the oral administration, animal subject shows no obvious abnormalities reaction.Gold shadow sunscreen cream rat oral median lethal dose(LD 50) (LD50) male and female belong to actual nontoxic level all greater than 5000mg/kg.
Acute dermal toxicity (LD50): behind the percutaneous dosing, animal subject shows no obvious abnormalities reaction.Gold shadow sunscreen cream rat percutaneous median lethal dose(LD 50) (LD50) male and female belong to actual nontoxic level all greater than 2000mg/kg.
According to the golden shadow sunscreen cream that preparation method of the present invention is produced, entrust toxicity teaching and research room of HSPH of Mountain Western Medicine S University that this product has been carried out rabbit skin irritant test.
Duration of test, intact skin group and damaged skin group rabbit control sides skin all do not have erythema and edema, and golden shadow sunscreen cream skin group does not also all have erythema and edema.According to the skin irritation standards of grading, marking to divide equally is 0 (0-0.49 is a nonirritant), so golden shadow sunscreen cream is to rabbit skin nonirritant.
According to the golden shadow sunscreen cream that preparation method of the present invention is produced, entrust toxicity teaching and research room of HSPH of Mountain Western Medicine S University that this product has been carried out the guinea pig skin hypersensitive test.
Gold shadow sunscreen cream treated animal skin does not all have redness, and its sensitization rate is 0, and positive controls sensitization rate is 100%, so golden shadow sunscreen cream does not have sensitization.
Above test report is referring to adnexa.
Sunlight screening skin-protecting product of the present invention has adopted specific band (UV-A, UV-B:200-400nm) sun-proof marine organisms material---oligonucleotide molecules; Can avoid the skin sense of discomfort of using chemical sunscreen to cause, not only anti-sunburn but also anti-tanned.The present invention makes full use of the character of oligonucleotide molecules to the special absorption of ultraviolet, with its add to can effectively stop in the skin care item cause human body skin damnification in, short wave ultraviolet; The ultraviolet of allowing some physiological neeies simultaneously passes through, the biological function of bringing into normal play.On biology is sun-proof, added opaque sun composition again---nanometer titanium folding powder, formed one deck effective isolation protective layer at skin surface, under the duplicate protection layer, prevent that effectively skin from being tanned severely.
The present invention makes an addition to the bioactive substance oligonucleotide molecules of extracting in the marine organism in the common skin care item, sun-proof result is remarkable, compare with chemical sunscreen with existing physics, can not cause stimulation to human body skin, therefore having efficient, green characteristics, is a kind of brand-new biological sunlight screening skin-protecting product.
The specific embodiment
Adopt biotechnology in the sardine body, to extract oligonucleotide molecules: sardine is cleaned, be ground into pasty state, add the distilled water of 5 times of volumes, carry out centrifugal treating behind the stirring and evenly mixing, get the upper strata water after the centrifugal treating, boiled 8-12 minute, carry out the centrifugal treating second time after the cooling again, collect the upper strata water then, every 1000ml adds non-specific Cobra venom endonuclease 2-3mg, act on 8 hours down at 37 ℃, promptly get oligonucleotide chromatography sample.The centrifugal force that two times centrifugal is handled is 3500-4500g, and the processing time is 20-30 minute.
Oligonucleotide chromatography sample is crossed the medium pressure chromatography post, adopting the DEAE-cellulose is filler, chromatographic column: 1 * 20cm, flow velocity: 10ml/min, with concentration is the gradient NaCl solution stepwise elution of 0.1-1.0mol/L, the 260nm ultraviolet detection, collecting with 0.6mol/L NaCl, pH7.4 is the eluent of eluting phase time; Eluent carries out supersound process behind the dialysis desalination, ultrasonic power is 200-500w, and the time is 1-3 hour, carries out lyophilisation at-50 ℃ then, obtains the oligonucleotide dry powder that purity reaches 87%-90%, dry powder yield 0.1-0.2%.
Above-mentioned eluent dialysis desalination, the employing molecular cut off is 1000 dialyzer, adopts distilled water to make dialysis solution.
The compound method of sunlight screening skin-protecting product of the present invention: pass through orthogonal experiment, determine the oligonucleotide content of adding and the proportion compatibility of skin-care product base, the mass ratio of oligonucleotide dry powder with 1: 1000 added in the base material of skin care item, and skin-care product base adopts the prescription of emollient cream.The common process flow process of producing according to skin care item during preparation, the finished product of sunlight screening skin-protecting product is promptly made in the back fill that stirs.This sunlight screening skin-protecting product can be made cream cream or water preparation, is used to smear or sprays the solarization skin surface.Experimental results show that this compatibility relationship can reach effect effectively sun-proof and the prevention relevant disease.
Above-mentioned emollient cream generally is to adopt Cera Flava, spermaceti, Brazil wax, cocoa butter, Oleum Camelliae, turtle oil, lanoline, cholesterol, hexadecanol, octadecanol, stearic acid and essence and water etc., under the effect of emulsifying agent, forms through stirring.
Mix mass ratio in the above-mentioned sunlight screening skin-protecting product and be 1: 2000 nanometer titanium folding powder,, further strengthen sun-proof result so that skin surface has formed one deck isolated protective layer.
Claims (5)
1, a kind of process for preparing novel sun-proof skin care is characterized in that:
A, employing biotechnology are extracted oligonucleotide molecules in the sardine body: sardine is cleaned, be ground into pasty state, add the distilled water of 5 times of volumes, carry out centrifugal treating behind the stirring and evenly mixing, get the upper strata water after the centrifugal treating, boiled 8-12 minute, carry out the centrifugal treating second time after the cooling again, collect the upper strata water then, every 1000ml adds non-specific Cobra venom endonuclease 1-4mg, act on 6-9 hour down at 37 ℃, promptly get oligonucleotide chromatography sample;
Oligonucleotide chromatography sample is crossed the medium pressure chromatography post, is the gradient NaCl solution stepwise elution of 0.1-1.0mol/L with concentration, and collecting with 0.5-0.8mol/L NaCl, pH7.4 is the eluent of eluting phase time; Eluent carries out supersound process behind the dialysis desalination, lyophilization then obtains oligonucleotide dry powder;
The compound method of B, sunlight screening skin-protecting product: oligonucleotide dry powder is added with 1: 1000 mass ratio in the base material of skin care item, and the common process flow process that adopts skin care item to produce stirs, fill, promptly makes the finished product of sunlight screening skin-protecting product.
2, process for preparing novel sun-proof skin care according to claim 1 is characterized in that the centrifugal force that above-mentioned two times centrifugal is handled is 3500-4500g, and the processing time is 20-30 minute.
3, process for preparing novel sun-proof skin care according to claim 1, the power that it is characterized in that above-mentioned supersound process is 200-500w, the time is 1-3 hour.
4, process for preparing novel sun-proof skin care according to claim 1 is characterized in that above-mentioned eluent dialysis desalination, and the employing molecular cut off is 1000 dialyzer, adopts distilled water to make dialysis solution.
5, process for preparing novel sun-proof skin care according to claim 1 is characterized in that mixing in the above-mentioned sunlight screening skin-protecting product mass ratio and is 1: 2000 nanometer titanium folding powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310123608 CN1226992C (en) | 2003-12-09 | 2003-12-09 | Preparation method of sun-screening and skin care product |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200310123608 CN1226992C (en) | 2003-12-09 | 2003-12-09 | Preparation method of sun-screening and skin care product |
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| CN1545997A true CN1545997A (en) | 2004-11-17 |
| CN1226992C CN1226992C (en) | 2005-11-16 |
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| CN 200310123608 Expired - Fee Related CN1226992C (en) | 2003-12-09 | 2003-12-09 | Preparation method of sun-screening and skin care product |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265116A (en) * | 2015-05-26 | 2017-01-04 | 徐加英 | A kind of preparation method of novel sun-proof skin care |
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2003
- 2003-12-09 CN CN 200310123608 patent/CN1226992C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265116A (en) * | 2015-05-26 | 2017-01-04 | 徐加英 | A kind of preparation method of novel sun-proof skin care |
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| CN1226992C (en) | 2005-11-16 |
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