CN1522753A - Use of glede tea aqueous extract in preparation of diabetes preventing and curing drug - Google Patents
Use of glede tea aqueous extract in preparation of diabetes preventing and curing drug Download PDFInfo
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- CN1522753A CN1522753A CNA031582311A CN03158231A CN1522753A CN 1522753 A CN1522753 A CN 1522753A CN A031582311 A CNA031582311 A CN A031582311A CN 03158231 A CN03158231 A CN 03158231A CN 1522753 A CN1522753 A CN 1522753A
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- actinodaphnes cupularis
- radix actinodaphnes
- water extract
- diabetes
- blood sugar
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Abstract
The present invention relates to a new application of aqueous extract of the Chinese medicinal materials laoyingcha in pharmaceutical field. Said laoyingcha aqueous extract can obviously reduce blood sugar value, and can obviously resist the blood sugar elevation due to adrenalin, so that it is applicable to all the types of diabetes, and its therapeutic effect is reliable, and its toxic side effect is less, and it adopts oral administration, its administration is convenient.
Description
Technical field
The present invention relates to the purposes of Radix Actinodaphnes Cupularis water extract, exactly is the purposes in pharmaceutical field.
Background technology
Radix Actinodaphnes Cupularis is a kind of conventional beverage in rural area, China south of the River, is subjected to liking and welcoming of drinking person over the past thousands of years deeply, and reason is abundant raw material, and processing is simple, is convenient to keeping, drinks conveniently, and is a kind of nontoxic, free of contamination pure natural beverage.In recent years, because environment is subjected to pollution to a certain degree, the consciousness of people's back to nature constantly increases, and this pure natural beverage more and more is subjected to people's favor.In order to be familiar with and to understand this wild natural resources, better develop and use, existing that the research and development review of Present Situation of Radix Actinodaphnes Cupularis is as follows.
The botany classification of Radix Actinodaphnes Cupularis: belong to Lauraceae, Litsea, hair Litsea coreana, be commonly called as " Ramulus et Folium Mussaendae Pubescentis ", also be Radix Actinodaphnes Cupularis.Formal name used at school Litsa coreana Leve.var. is a kind of evergreen dungarunga or arbor.Often be grown in the low mountain evergreen broadleaf forest of about 1000 meters of height above sea level, be distributed in ground such as Sichuan, Guizhou, Anhui, Yunnan, Chongqing, Hunan and Jiangxi.
Annual shoot puce or green have curling pubescence, hole skin yellowish-brown or green, and branch was bald clean in 1 year.Bud is avette, outer green of scale or yellowish-brown thin,tough silk hair.
Leaf keratin, alternate, ellipse, narrow ellipse or the shape of falling ovum ellipse, tip be point or prominent point gradually, the base portion wedge shape, and long 5-15cm, wide 1.5-5.cm, back side canescence has curling pubescence, and is bald sometimes clean, lateral vein 6-10 bar, the long 0.5-1.5cm of petiole, hairiness.Under natural environment, a year sprouting sprouting 2 is taken turns, and the 4-5 month is the spring bud, and the 8-9 month is the autumn bud, is height with spring bud output.
Flower, wax yellow, inflorescence do not have stalk, and several cluster at axil, and involucre has undercoat outward, and every preface has colored 4-8, flower diameter 4-5mm.
Really, closely spherical (oblong is arranged and falls oval), the about 6mm of diameter, carpopodium is sturdy, long 0.5-0.8mm, hairiness, hypocarp is flat expands, the perianth sliver that has the place to deposit.The florescence 8-9 month, fruit 5-8 month next year phase.
Seed, by the thin peel parcel of one deck, seed is hard, and waxiness is arranged.The about 6mm of seed diameter.
The chemical constituent of Radix Actinodaphnes Cupularis:
Contain rich in amino acid in the Radix Actinodaphnes Cupularis, total content is that 0.82%, 8 kind of essential amino acid is complete, and content is the highest with leucine, and lysine takes second place, and methionine is minimum, and this is similar with green tea, and wherein methionine content is also a little more than green tea.In non essential amino acid, the highest with glutamic acid content, secondly be aspartic acid and arginine, compare no significant difference with green tea.From total amino acid content, the aminoacid of green tea is higher than Radix Actinodaphnes Cupularis, its main cause is the content height of peculiar theanine in the green tea, theanine reflects the sensory difference of green tea and Radix Actinodaphnes Cupularis to a certain extent, illustrates also that on the other hand former plant of green tea and Radix Actinodaphnes Cupularis plant have different amino acid metabolism types.
Radix Actinodaphnes Cupularis is rich in the multi mineral prime element, wherein potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), sodium (Na), zinc (Zn), ferrum (Fe), selenium (Se) all are the nutrients of needed by human, especially zinc (Zn), ferrum (Fe), selenium (Se) constituent content are higher than green tea, and hemopoietic, brain-strengthening are had better effect.The content that it will be further appreciated that harmful element lead is starkly lower than green tea.
The water soluble vitamins that Radix Actinodaphnes Cupularis contains mainly is Vc, V
B1And V
B2, its content is lower slightly than green tea, may be relevant with factors such as the course of processing and metabolic pathways.
Total flavones (TF in the Radix Actinodaphnes Cupularis, total flavonoid) content is far above green tea, green tea has only about 1.22%, and Radix Actinodaphnes Cupularis can be up to 3.45%, that has determined has a nimbecetin (kaempferol), nimbecetin-3-O-β-D-glucoside (kaempferol-3-O-β-D-glucopyranoside), Quercetin-3-O-β-D-glucoside (quercetin-3-O-β-D-glucopyranoside).
Radix Actinodaphnes Cupularis obtains light yellow oily liquid, aromatic flavor, oil yield about 0.1% through the volatile ingredient of atmospheric steam distillation extraction Radix Actinodaphnes Cupularis.Through the gas chromatography-mass spectrography analysis, identify 32 kinds of compositions (wherein terpenoid and derivant thereof are 20 kinds), account for 95% of volatile oil composition, main component is that n-capric aldehyde (decanal) content is 71.53%; Next is 10-alkene-hendecanal (10-undecenal), and content is 5.02%; Aldehyde C-9 (n-nonaldehyde), content 3.58%; Positive dodecoic acid vinyl acetate (dodecanoic acid, ethenyl ester), content 2.63%.The main component of fragrance is a terpenoid, wherein mainly is that molecular weight is 204 terpenoid.
Also contain polysaccharide, Saponin, polyphenol and natural pigment etc. in the Radix Actinodaphnes Cupularis.
In addition, what deserves to be mentioned is especially that compare with green tea, the chemical constituent of Radix Actinodaphnes Cupularis does not contain the caffeine material, no excitation does not influence sleep, a kind of especially good selection of people that this takes in needs control caffeine.
Radix Actinodaphnes Cupularis (Litsea coreana Leve.var.) is drunk long-standing in vast rural area, China south of the River, be subjected to liking of numerous working people deeply, but as just a kind of beverage.In recent years, ground such as Sichuan, Guizhou have carried out some developments and utilizations, but also only limit to roughing, also are not fully recognized that its pharmacological function, and its potential pharmacological mechanism does not more have forefathers and inquires into.According to bibliographical information, long-term drink have anti-sour, anticorrosion, relieve summer heat quench one's thirst, promoting digestion and removing stagnation, detoxifcation, swelling diuretic, make eye bright, be good for the stomach, the effect of benefit think of.
The object of the present invention is to provide the new purposes of Radix Actinodaphnes Cupularis water extract, i.e. new application in pharmacy.
In fact, the invention provides the application of Radix Actinodaphnes Cupularis water extract in the medicine of preparation treatment or prevent diabetes.
Along with the prolongation of human longevity, the improving constantly of living standard, the sickness rate of diabetes (diabetes) is rising year by year, and has very high disability rate and fatality rate, and human beings'health in serious threat.According to conservative estimation, the patient of whole world diabetes is nearly 400,000,000, and China nearly just has 50,000,000.World Health Organization (WHO) will prevent and treat this class disease and classify one of major issue of global human health as.
In recent years, the drug development of treatment diabetes is very fast.The medicine of treatment diabetes has:
A, insulin are mainly used in type i diabetes, the II diabetes are under the emergency rating through diet and the not controlled person of other medicines, diabetic complication and body.But because it is the polypeptide class, necessary drug administration by injection brings many inconvenience to the patient, also has potential danger: (1) hyperinsulinemia; (2) weight increase; (3) hypoglycemia.
B, biguanides, be mainly used in the fat type ii diabetes patient that fails to control through diet and exercise therapy or with the other medicines use in conjunction.Its modal untoward reaction is a gastrointestinal reaction, and serious adverse effects is lactic acidosis and nephrotoxicity.
C, sulfonylurea, the tight diet exercise therapy of the NIDDM of morbidity above middle age fail Satisfactory Control person or with the other drug coupling.Its untoward reaction mainly contains: skin allergy, gastrointestinal reaction and hypoglycemia etc.
D, alpha-glucosidase inhibitor, being mainly used in post-prandial glycemia can not Satisfactory Control person, but occurs abdominal distention, stomachache and constipation etc. easily.
E, euglycemic agent, clinical practice is in the II diabetics.Though untoward reaction is few, the price comparison costliness.
Radix Actinodaphnes Cupularis water extract 900mg/kg can significantly reduce the blood glucose value of normal mouse, (comparing p＜0.05 with matched group).The Radix Actinodaphnes Cupularis water extract all can significantly resist the mouse blood sugar that is caused by epinephrine and raise (comparing p＜0.05 with model group), and wherein the blood sugar reducing function with 100mg/kg significantly (compares p＜0.01 with model group).Radix Actinodaphnes Cupularis water extract 300mg/kg, 900mg/kg can significantly reduce the blood glucose value (comparing p＜0.05 with model group) of alloxan diabetes mice.
In order to understand essence of the present invention better, will its new purposes in pharmaceutical field be described with the pharmacological evaluation and the result of Radix Actinodaphnes Cupularis water extract below.
The hypoglycemic activity test of Radix Actinodaphnes Cupularis water extract is as follows:
(1) material
Animal
Kunming mouse, male and female half and half, body weight 18-22g is provided by this school Experimental Animal Center.Fasting 2h before all referring to test when unreceipted in the experiment.
Medicine and reagent
The Radix Actinodaphnes Cupularis water extract, self-control;
Glibenclamide (glyburide, glibenclamide): Tianjin Pacific Pharmaceutical Co., Ltd., lot number 020907.
Phenformin (phenformin, insoral, phenfarmin): Nantong pharmacy head factory, lot number 011017.
Alloxan (Alloxan): Sigma company product, face with preceding and be mixed with 1% solution with normal saline.
Adrenalin hydrochloride injection: Shanghai Hefeng Pharmaceutical Co., Ltd., lot number 021202-7.
Glucose (Glu) is measured test kit: Bioisystech Co., Ltd's product is promoted in glory in Shanghai.
(2) method
The preparation of Radix Actinodaphnes Cupularis water extract
Take by weighing Radix Actinodaphnes Cupularis 100g, add 2000 milliliters of distilled water, ultrasonic 30 minutes, isolated by filtration, keep supernatant, filtering residue adds 2000 milliliters of distilled water again, repeats above step, merges 2 times supernatant, 80 ℃ of water-baths oven dry of spending the night behind the concentrating under reduced pressure, 50 ℃ of vacuum dryings are weighed.
The Radix Actinodaphnes Cupularis water extract is to the influence of normal mouse blood sugar
50 of normal mouses divide 5 groups at random, gastric infusion (Radix Actinodaphnes Cupularis water extract 100mg/kg, 300mg/kg, 900mg/kg, glyburide 50mg/kg) or equal-volume normal saline (20ml/kg), and every day 1 time, continuous 5d, 3h gets blood and surveys blood sugar content after the last administration.
The Radix Actinodaphnes Cupularis water extract is to the influence of adrenal gland's disposition hyperglycemia mouse blood sugar
60 of normal mouses divide 6 groups at random, gastric infusion (Radix Actinodaphnes Cupularis water extract 100mg/kg, 300mg/kg, 900mg/kg, glyburide 50mg/kg) or equal-volume normal saline (normal control, model), every day 1 time, continuous 5d, 1h after the last administration, normal control group ip normal saline, all the other 5 groups equal ip epinephrine 300ug/kg got blood in back 30 minutes in injection and survey blood sugar content.
The Radix Actinodaphnes Cupularis water extract is to the influence of alloxan diabetes mouse blood sugar
60 of normal mouses, except that 10 normal control groups, all the other equal tail vein injection 1% alloxan normal saline 100mg/kg cause diabetes model, (fasting 12h) divides 5 groups, gastric infusion (Radix Actinodaphnes Cupularis water extract 100mg/kg, 300mg/kg, 900mg/kg, insoral 100mg/kg) or equal-volume normal saline after getting blood prediction blood glucose behind the 72h, every day 1 time, continuous 5d, fasting 16h before the last administration, 1h gets blood and surveys blood sugar content after the last administration.
(3) result
The Radix Actinodaphnes Cupularis water extract is to the influence of normal mouse blood sugar
By table 1 as seen, the Radix Actinodaphnes Cupularis water extract all can reduce the blood glucose value of normal mouse, wherein with 900mg/kg blood sugar reducing function significantly (p＜0.01).
Table 1 Radix Actinodaphnes Cupularis water extract to the influence of normal mouse blood sugar (x ± s, n=10)
Group dosage (mg/kg) blood glucose (mmol/l)
Contrast-7.20 ± 0.60
Radix Actinodaphnes Cupularis water extract 100 6.92 ± 1.60
300 6.48±1.53
900 5.78±1.32**
Glyburide 50 6.08 ± 0.87*
* compare with matched group, p＜0.05 * * and matched group compare, p＜0.01
The Radix Actinodaphnes Cupularis water extract is to the influence of adrenal gland's disposition hyperglycemia mouse blood sugar
The Radix Actinodaphnes Cupularis water extract all can significantly resist the mouse blood sugar that caused by epinephrine and raise, and wherein with the blood sugar reducing function of 100mg/kg significantly (p＜0.01), the results are shown in Table 2.
Table 2 Radix Actinodaphnes Cupularis water extract to the influence of adrenal gland's disposition hyperglycemia mouse blood sugar (x ± s, n=10)
Group dosage (mg/kg) blood glucose (mmol/l)
Contrast-6.42 ± 1.66
Model-10.43 ± 2.51
Δ
Radix Actinodaphnes Cupularis water extract 100 6.66 ± 1.53**
300 7.77±1.42*
900 7.11±2.53*
Glyburide 50 5.86 ± 2.05**
ΔCompare with matched group, p＜0.01 * * and model group compare, p＜0.01
* compare p＜0.05 with model group
The Radix Actinodaphnes Cupularis water extract is to the influence of alloxan diabetes mouse blood sugar
The Radix Actinodaphnes Cupularis water extract all can reduce the blood glucose value of alloxan diabetes mice, wherein with the blood sugar reducing function of 300mg/kg, 900mg/kg significantly (p＜0.05), the results are shown in Table 3.
Table 3 Radix Actinodaphnes Cupularis water extract to the influence of alloxan diabetes mouse blood sugar (x ± s, n=10)
Group dosage (mg/kg) blood glucose (mmol/l)
Contrast-7.35 ± 1.54
Model-26.71 ± 3.96
Δ
Radix Actinodaphnes Cupularis water extract 100 23.98 ± 5.14
300 21.68±4.96*
900 21.52±1.82*
Insoral 100 19.34 ± 6.22*
ΔCompare with matched group, p＜0.01 * * and model group compare, p＜0.01
* compare p＜0.05 with model group
Can draw the advantage that the present invention has several respects from above-mentioned result of the test:
1, the present invention has excavated new prospect in medicine to the Radix Actinodaphnes Cupularis water extract, has opened up a new application.
2, Radix Actinodaphnes Cupularis water extract of the present invention adopts oral administration, taking convenience, and toxic and side effects is little, takes medicine for a long time and does not have harm.
3, Radix Actinodaphnes Cupularis water extract of the present invention is applicable to various diabetes, acts on a plurality of links, determined curative effect.
4, the raw material resources of Radix Actinodaphnes Cupularis water extract of the present invention are abundant, have good society and economic benefit.
5, can the present invention carry out further new drug research exploitation for the basis.
The specific embodiment
Below in conjunction with embodiment the present invention is done to describe further.
Embodiment:
Take by weighing Radix Actinodaphnes Cupularis 100g, add 2000 milliliters of distilled water, ultrasonic 30 minutes, isolated by filtration, keep supernatant, filtering residue adds 2000 milliliters of distilled water again, repeats above step, merge 2 times supernatant, 80 ℃ of water-baths oven dry of spending the night behind the concentrating under reduced pressure, 50 ℃ of vacuum dryings promptly get yellowish-brown graininess Radix Actinodaphnes Cupularis water extract, has fragranced, easily deliquescence.
Eagle tea is got thing and is recommended clinical dosage: 600-5400mg/ day, and oral, divide and take for three times.
Claims (1)
1, the application of Radix Actinodaphnes Cupularis water extract in pharmacy is characterized in that: the application of Radix Actinodaphnes Cupularis water extract in preparation control diabetes medicament.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03158231 CN1233386C (en) | 2003-09-11 | 2003-09-11 | Use of glede tea aqueous extract in preparation of diabetes preventing and curing drug |
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| CN 03158231 CN1233386C (en) | 2003-09-11 | 2003-09-11 | Use of glede tea aqueous extract in preparation of diabetes preventing and curing drug |
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| CN1522753A true CN1522753A (en) | 2004-08-25 |
| CN1233386C CN1233386C (en) | 2005-12-28 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106383A (en) * | 2011-01-18 | 2011-06-29 | 合肥工业大学 | Litsea coreana yoghourt and preparation method and application thereof |
| CN103504374A (en) * | 2013-09-23 | 2014-01-15 | 安徽凯利粮油食品有限公司 | Processing method for healthcare snakegourd fruit seed |
| CN104336775A (en) * | 2013-07-26 | 2015-02-11 | 江西中烟工业有限责任公司 | Hawk tea extract, preparation method thereof and application of extract in cigarette |
| CN105055522A (en) * | 2015-07-27 | 2015-11-18 | 合肥一诺生物科技有限公司 | Application of hawk tea water extract in preparing medicine for invigorating stomach and helping help digestion |
| CN115894735A (en) * | 2022-12-07 | 2023-04-04 | 遵义医科大学 | Litsea coreana acidic polysaccharide, and extraction and purification method and anticancer application thereof |
| CN115894735B (en) * | 2022-12-07 | 2024-05-14 | 遵义医科大学 | Eagle tea acidic polysaccharide, extraction and purification method and anticancer application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028070A (en) * | 2010-12-20 | 2011-04-27 | 胡肇眉 | Health tea with hair blackening function |
-
2003
- 2003-09-11 CN CN 03158231 patent/CN1233386C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106383A (en) * | 2011-01-18 | 2011-06-29 | 合肥工业大学 | Litsea coreana yoghourt and preparation method and application thereof |
| CN102106383B (en) * | 2011-01-18 | 2012-06-13 | 合肥工业大学 | Litsea coreana yoghourt and preparation method and application thereof |
| CN104336775A (en) * | 2013-07-26 | 2015-02-11 | 江西中烟工业有限责任公司 | Hawk tea extract, preparation method thereof and application of extract in cigarette |
| CN103504374A (en) * | 2013-09-23 | 2014-01-15 | 安徽凯利粮油食品有限公司 | Processing method for healthcare snakegourd fruit seed |
| CN105055522A (en) * | 2015-07-27 | 2015-11-18 | 合肥一诺生物科技有限公司 | Application of hawk tea water extract in preparing medicine for invigorating stomach and helping help digestion |
| CN115894735A (en) * | 2022-12-07 | 2023-04-04 | 遵义医科大学 | Litsea coreana acidic polysaccharide, and extraction and purification method and anticancer application thereof |
| CN115894735B (en) * | 2022-12-07 | 2024-05-14 | 遵义医科大学 | Eagle tea acidic polysaccharide, extraction and purification method and anticancer application thereof |
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| Publication number | Publication date |
|---|---|
| CN1233386C (en) | 2005-12-28 |
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