CN102106383B - Litsea coreana yoghourt and preparation method and application thereof - Google Patents
Litsea coreana yoghourt and preparation method and application thereof Download PDFInfo
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- CN102106383B CN102106383B CN2011100098487A CN201110009848A CN102106383B CN 102106383 B CN102106383 B CN 102106383B CN 2011100098487 A CN2011100098487 A CN 2011100098487A CN 201110009848 A CN201110009848 A CN 201110009848A CN 102106383 B CN102106383 B CN 102106383B
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Abstract
The invention discloses Litsea coreana yoghourt, a preparation method and application thereof. The Litsea coreana yoghourt is prepared from the following raw materials: fresh milk or reconstituted milk, isomaltose hypgather, fructooligosaccharide, soybean oligosaccharide, sodium caseinate, Litsea coreana, Lactobacillus delbrueckii and Streptococcus thermophilus. The preparation method for the Litsea coreana yoghourt comprises the following steps of: adding the Litsea coreana serving as a functional factor into the fresh milk or the reconstituted milk, and inoculating lactic acid bacteria for fermentation. The Litsea coreana yoghourt can reduce blood sugar and improve immunity, improves the healthcare function value of yoghourt, and enriches the variety of the yoghourt.
Description
One, technical field
The present invention relates to a kind of functional form dairy products, specifically a kind of eagle boheic acid milk.
Two, background technology
Sour milk is a primary raw material with fresh milk or reconstituted milk normally, through homogeneous, pasteurize or sterilization, and a kind of fermented dairy product that is rich in probio of prepared such as lactobacillus-fermented.Streptococcus thermophilus in Yoghourt Production (ys14) and lactobacillus delbruockii subspecies bulgaricus (1.1480) are the bacterial strains of using always, and they have good synergy.Sour milk not only contains nutriments such as abundant calcium, protein, riboflavin and vitamin, but also contains a large amount of probios useful to health.Confirmed that at present sour milk has the balance gut flora, improves immunity of organisms, reduces cholesterol and effect such as delay senility human body, Along with people's improves constantly the requirement of dairy products in addition, and the function dairy products cause scholars' extensive concern.The function dairy products of having reported have anti-constipation dairy products, reducing blood lipid, anti-ageing year dementia dairy products, antitumor dairy products, the dairy products of treating osteoporotic dairy products, improvement sleep and anti-diabetic dairy products or the like.In sour milk, add compositions such as trivalent chromium, summer cypress and sealwort and prepare hypoglycemic sour milk.Yet with eagle tea is that feedstock production functional form sour milk does not appear in the newspapers.
Eagle tea, promptly hair leopard camphor tree (Lietsea coreana L.) belongs to the Lauraceae Litsea, is medicinal and edible plant.It is a kind of plant alternative tea of southern china long-term drinking among the people; Also be the traditional Chinese medicine of putting down in writing in the Compendium of Material Medica, have removing toxicity for detumescence, the benefit that makes eye bright is thought, anti-oxidant, hypoglycemic, reducing blood lipid and improve function such as liver lipid-metabolism imbalance and have no side effect.The researcher studies part mineral composition, chemical composition, amino acid, flavone compound and the polyphenolic substance etc. of eagle tea; And find that the eagle tea set has the hypoglycemic functions such as blood fat and the imbalance of liver lipid-metabolism of improving, and relates to but use eagle tea not have as development of raw materials functional form dairy products.
Three, summary of the invention
The present invention is the weak point that exists for fear of above-mentioned prior art, and a kind of eagle boheic acid milk is provided.The technical problem that solves is to be function factor with eagle tea, make an addition in fresh milk or the reconstituted milk, and the inoculating lactic acid bacterium fermenting process of preparing has the eagle boheic acid milk of blood sugar decreasing effect, is worth and has enriched the kind of sour milk with the health care that improves sour milk.
Technical solution problem of the present invention adopts following technical scheme:
The characteristics of eagle boheic acid milk of the present invention are to be suckled through the eagle boheic acid that fermentation makes by the microorganism of each raw material of following mass percent and percentage inoculation by volume:
Component A: fresh milk or reconstituted milk 85-90%, oligoisomaltose 3-4%, FOS 1.5-2%, soyabean oligosaccharides 0.5-1%, casein sodium 0.6-1.2%;
Component B: eagle tea 4-8%;
Component C: lactobacillus yogurt leavening, inoculum concentration are 1.0g/L.
The preparation method's of eagle boheic acid milk of the present invention characteristics are to operate according to the following steps:
A, with each raw material blending among the component A, under 50-60 ℃, 15-20MPa homogeneous 10-15 minute, add component B eagle tea, sterilization, be cooled to after filtering 42 ℃ mixed emulsion;
B, in said mixed emulsion inoculation component C lactobacillus yogurt leavening, 40-42 ℃ ferment to pH be 4.5-4.8, again in 4 ℃ of refrigerations 12-24 hour, promptly get the eagle boheic acid and suckle.
The preparation method's of eagle boheic acid milk of the present invention characteristics also are: said sterilization is 80-85 ℃ of insulation 35 minutes or 95-100 ℃ of insulation 10 minutes.
The preparation method's of eagle boheic acid milk of the present invention characteristics also are: said filtration was aseptic degreasing cotton.
The characteristics of the purposes of eagle boheic acid milk of the present invention are: the application of said eagle boheic acid milk aspect reduction blood sugar and raising immunity.
The relevant regulations of eagle boheic acid that this method makes milk main technical requirements National standard GB 2746-1999, protein content>=2.5% wherein, lactic acid bacteria number＞1 * 10
7Cfu/mL.
The eagle boheic acid hypoglycemic animal experiment of suckling:
1, material
The eagle boheic acid milk of the present invention's preparation;
The small white mouse feed, Medical University Of Anhui's animal center provides;
Male Kunming small white mouse 20 ± 2g, Medical University Of Anhui's animal center provides;
Alloxan, Beijing Suo Laibao Science and Technology Ltd.;
Electronic balance, Pu, Shanghai spring metering Co., Ltd;
Luo Shi blood glucose meter and Luo Shi blood sugar test paper are bought common people's edge pharmacy in Hefei;
Syringe, Shanghai Jinta Medical Equipment Co., Ltd..
2, method
A, set up diabetes small white mouse model
After small white mouse adaptability fed a week, begin to set up diabetes model.With behind the strict fasting 24h of small white mouse (water is can't help in fasting, changes bedding and padding), the alloxan physiological saline of lumbar injection 2% (dosage 200mg/kg body weight), the physiological saline of blank control group small white mouse injection corresponding dosage.Recover normal diet after the injection immediately, behind 72h, again small white mouse is carried out fasting in 12 hours (can't help water, note change bedding and padding), whole small white mouse fasting blood sugars are measured in the docking blood sampling, select blood glucose value more than 15mmol/L as the diabetes model small white mouse.Small white mouse is divided into groups again, and feed 30 days continuously again.
Grouping of b, small white mouse and nursing
Blank group (C), healthy mice are fed and are raised normal diet.
Hyperglycemia model control group (M), diabetes small white mouse are fed and are raised normal diet.
Test 1 group (TYI), the diabetes small white mouse is irritated the eagle boheic acid milk (containing eagle tea 4%) that is fed with 1mL/20g dosage.
Test 2 groups (TYH), the diabetes small white mouse is irritated the eagle boheic acid milk (containing eagle tea 8%) that is fed with 1mL/20g dosage.
C, detection blood glucose value
Begin from setting up hyperglycemia model and formally feeding small white mouse, at the 10th day, 20 days, 30 days blood glucose values, all want strict fasting 12h before the detection but the assurance drinking water supply respectively with docking blood taking method detection small white mouse with DIFFERENT FEED.
D, thymus gland and index and spleen index
Thymus index=(thymus gland/body weight) * 100%; Index and spleen index=(spleen/body weight) * 100%.
3, result
Can know hyperglycemia model control group small white mouse blood glucose value greater than 15mmol/L from table 1, compare significantly (P＜0.01) increase explanation modeling success of blood glucose value with contrast.The model small white mouse is divided into 3 groups every group 6 at random.Be increase trend at 0-30d model group blood glucose value.Experimental group 1 and 2 small white mouses feed eagle boheic acid milk 20 with 30d blood sugar than model group blood sugar all significantly (P＜0.05) descend; Blood glucose value also has decline in various degree before irritating hello eagle boheic acid milk more on the same group in addition; Best result has not descended 5.8% and 7.9%, and this shows that eagle boheic acid milk has hypoglycemic function.
Table 1 eagle boheic acid milk is to the influence
of glycosuria and small white mouse blood sugar
Annotate: m, M represent that respectively the model group of respective column and control group have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference;
A, A represent that respectively the experimental group 1 and the model group of respective column have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference;
B, B represent that respectively the experimental group 2 and the model group of respective column have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference;
Blood sugar had conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference before g, G represented on the same group not on the same day blood sugar respectively and give sour milk.
Can know that by table 2 thymus index and the index and spleen index of model group small white mouse all significantly descend than control group, show that the immunocompetence of diabetes small white mouse descends.And thymus index and the index and spleen index of experimental group 1 and experimental group 2 small white mouses have increase in various degree than model group, and experimental group 1 and 2 small white mouse thymus gland and index and spleen index contrast the group no significant difference in addition, show that eagle boheic acid milk can improve small white mouse immunity.
Table 2 eagle boheic acid milk is to the influence of small white mouse immune organ index
Annotate: m, M representation model group and control group respectively have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference;
A, A represent that respectively experimental group 1 and model group have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference;
B, B represent that respectively experimental group 2 and model group have conspicuousness (P＜0.05) and highly significant property (P＜0.01) difference.
Four, the specific embodiment
Non-limiting embodiment is narrated as follows:
Embodiment 1:
1, gets the raw materials ready
Component A: fresh milk 85%, oligoisomaltose 3%, FOS 2%, soyabean oligosaccharides 1%, casein sodium 1.2%;
Component B: eagle tea 7.6%;
Component C: lactobacillus yogurt leavening, inoculum concentration are 1.0g/L.
2, preparation
A, with each raw material blending among the component A, homogeneous is 15 minutes under 50 ℃, 15MPa, adds eagle tea, in 80-85 ℃ of insulation sterilization in 35 minutes, being cooled to 42 ℃ after filtering with aseptic cotton balls must mixed emulsion;
B, in said mixed emulsion the inoculating lactic acid bacterium ferment agent for sour milk, 40 ℃ ferment to pH be 4.5, in 4 ℃ of refrigerations 24 hours, promptly get the eagle boheic acid and suckle again.
The eagle boheic acid milk of present embodiment preparation has the effect that reduces blood sugar and raising immunity.
Embodiment 2:
1, gets the raw materials ready
Component A: reconstituted milk 90%, oligoisomaltose 3.4%, FOS 1.5%, soyabean oligosaccharides 0.5%, casein sodium 0.6%;
Component B: eagle tea 4%;
Component C: lactobacillus yogurt leavening, inoculum concentration are 1.0g/L.
2, preparation
A, with each raw material blending among the component A, homogeneous is 10 minutes under 60 ℃, 20MPa, adds eagle tea, in 95-100 ℃ of insulation sterilization in 10 minutes, being cooled to 42 ℃ after filtering with aseptic cotton balls must mixed emulsion;
B, in said mixed emulsion the inoculating lactic acid bacterium ferment agent for sour milk, 40-42 ℃ ferment to pH be 4.8, in 4 ℃ of refrigerations 12 hours, promptly get the eagle boheic acid and suckle again.
The eagle boheic acid milk of present embodiment preparation has the effect that reduces blood sugar and raising immunity.
Embodiment 3:
1, gets the raw materials ready
Component A: fresh milk 86%, oligoisomaltose 4%, FOS 1.8%, soyabean oligosaccharides 0.7%, casein sodium 1%;
Component B: eagle tea 6.5%;
Component C: lactobacillus yogurt leavening, inoculum concentration are 1.0g/L.
2, preparation
A, with each raw material blending among the component A, homogeneous is 12 minutes under 55 ℃, 18MPa, adds eagle tea, in 80-85 ℃ of insulation sterilization in 35 minutes, being cooled to 42 ℃ after filtering with aseptic cotton balls must mixed emulsion;
B, in said mixed emulsion the inoculating lactic acid bacterium ferment agent for sour milk, 40-42 ℃ ferment to pH be 4.6, in 4 ℃ of refrigerations 18 hours, promptly get the eagle boheic acid and suckle again.
The eagle boheic acid milk of present embodiment preparation has the effect that reduces blood sugar and raising immunity.
The lactobacillus yogurt leavening that embodiment 1-3 uses is bought the show Science and Technology Ltd. in river, Beijing.
Claims (5)
1. an eagle boheic acid is suckled, and it is characterized in that being suckled through the eagle boheic acid that fermentation makes by the microorganism of each raw material of following mass percent and percentage inoculation by volume:
Component A: fresh milk or reconstituted milk 85-90%, oligoisomaltose 3-4%, FOS 1.5-2%, soyabean oligosaccharides 0.5-1%, casein sodium 0.6-1.2%;
Component B: eagle tea 4-8%;
Component C: lactobacillus yogurt leavening, inoculum concentration are 1.0g/L.
2. the preparation method of eagle boheic acid milk as claimed in claim 1 is characterized in that operating according to the following steps:
A, with each raw material blending among the component A, under 50-60 ℃, 15-20MPa homogeneous 10-15 minute, add component B eagle tea, sterilization, be cooled to after filtering 42 ℃ mixed emulsion;
B, in said mixed emulsion inoculation component C lactobacillus yogurt leavening, 40-42 ℃ ferment to pH be 4.5-4.8, again in 4 ℃ of refrigerations 12-24 hour, promptly get the eagle boheic acid and suckle.
3. preparation method according to claim 2 is characterized in that: said sterilization is 80-85 ℃ of insulation 35 minutes or 95-100 ℃ of insulation 10 minutes.
4. preparation method according to claim 2 is characterized in that: said filtration was aseptic degreasing cotton.
5. the purposes of eagle boheic acid milk according to claim 1 is characterized in that: said eagle boheic acid milk reduces blood sugar and the application that improves in the immunity medicine in preparation.
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| CN2011100098487A CN102106383B (en) | 2011-01-18 | 2011-01-18 | Litsea coreana yoghourt and preparation method and application thereof |
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| CN2011100098487A CN102106383B (en) | 2011-01-18 | 2011-01-18 | Litsea coreana yoghourt and preparation method and application thereof |
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| CN102106383B true CN102106383B (en) | 2012-06-13 |
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| CN102422882B (en) * | 2011-10-21 | 2012-11-21 | 合肥工业大学 | Soybean-honey acidophilus milk and preparation method thereof |
| CN102823648B (en) * | 2012-07-31 | 2013-09-25 | 安徽省天旭茶业有限公司 | Jasmine tea yoghourt and preparation method thereof |
| CN102823649B (en) * | 2012-07-31 | 2013-09-25 | 安徽省天旭茶业有限公司 | Broadleaf holly leaf yoghourt and preparation method thereof |
| CN103875804B (en) * | 2012-12-21 | 2016-02-17 | 内蒙古伊利实业集团股份有限公司 | A kind of tea flavour Yoghourt and preparation method thereof |
| CN103190487A (en) * | 2013-03-07 | 2013-07-10 | 安徽詹氏食品有限公司 | Function-reinforced hickory nut milk and preparation method thereof |
| CN103190486A (en) * | 2013-03-07 | 2013-07-10 | 安徽詹氏食品有限公司 | Sugar-free hickory nut milk and preparation method thereof |
| CN103548997B (en) * | 2013-10-25 | 2015-11-18 | 刘冬 | A kind of Fuzhuan tea yoghourt and preparation method thereof |
| CN105309615B (en) * | 2015-11-30 | 2020-06-05 | 光明乳业股份有限公司 | Tea yoghurt and preparation method thereof |
| CN105494634A (en) * | 2015-12-17 | 2016-04-20 | 安庆职业技术学院 | Stirring type litsea coreana flavored fermented milk and making method thereof |
| CN106798063A (en) * | 2016-12-19 | 2017-06-06 | 蚌埠鲲鹏食品饮料有限公司 | A kind of fermented coffee beverage |
| CN106819117A (en) * | 2017-01-11 | 2017-06-13 | 安徽科技学院 | A kind of common rabdosia leaf Yoghourt and its production method |
| CN107410486A (en) * | 2017-06-27 | 2017-12-01 | 合肥工业大学 | A kind of preparation method of hypoglycemic roselle acidified milk |
| CN107197943A (en) * | 2017-06-27 | 2017-09-26 | 合肥工业大学 | A kind of preparation method of anti-oxidant cape jasmine fruit acidified milk |
| CN107467282A (en) * | 2017-08-30 | 2017-12-15 | 南京卫岗乳业有限公司 | One plant tea flavor lactacidase fermenting beverage and preparation method thereof |
| CN114470037A (en) * | 2022-03-04 | 2022-05-13 | 重庆医科大学 | Application of Litsea coreana extract in preventing and treating obesity and intestinal flora disorder |
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