CN1498899A - Vegetable protein as well as preparation method and application - Google Patents

Abstract

A plant protein used for feed, food, cosmetics, health-care products, etc. is prepared from the kernel of Ou plum through baking, peeling, pulverizing, proportionally mixing with petroleum ether, removing oil by refluxing in water bath at 40-60 deg.C while stirring, vacuum filter, dispering it in water, regulating pH value to 9.0-10, extracting for 15-45 min, centrifugal separation to obtain supernatant, vegulating pH value to 3.5-4.5 depositing to obtain protein, centrifugal deposition, dispersing the deposit in distilled water, regulating pH value to 6.5-7.5 and spray drying.

Description

A kind of vegetable-protein and production method thereof and application

Technical field

The present invention relates to a kind of vegetable-protein and production method thereof and application, particularly a kind of albumen and production method and application from xylophyta.

Background technology

Protein has irreplaceable vital role in edible, feeding, medical and other many light industry field, and the protein of taking in q.s is the prerequisite that animal depends on for existence.

Desertification is one of the serious environmental in the current whole world and social concern.The present desertification in China soil is very serious, and the desertification area of northern area is constantly expanded over nearly 50 years, has reached nearly 350,000 square kilometres, and is continuing expansion with annual 2460 square kilometres speed, and its rate of propagation has the trend that is quickening.Psammophyte has important effect in defending and controlling sand, excavate other economic worth of psammophyte, and ecological construction and industry development are combined, and with sand control cure sand, has important reality, society and economic implications.

Europe Lee (Cerasus humilis.BGE) is the psammophyte of rose department cherry genus.Former plant is a machaka, and root system is very flourishing, and root mass is huge, the about 0.5-1.5 rice of plant height, and the drought-enduring salt tolerant alkali that can resist cold, tool is good checks winds and fixes drifting sand, the soil conservation performance.Its fruit is contained nutritious, especially Vc, VB ₂, V _EAnd the content of potassium, calcium, phosphorus, iron, zinc, selenium and Methionin all is higher than existing common fruit.Europe Lee's kernel and Gen Pi are as traditional Chinese medicine, and its nature,taste and action all has clearly record in Compendium of Material Medica and " legendary god of farming's book on Chinese herbal medicine ".

Contain a large amount of greases among the Ou Liren, Europe Lee oil is formed best in quality, have well edible and nourishing function, at application number is in 02146618.1, denomination of invention is that the contriver describes the content of relevant Europe Lee's oil in detail, meanwhile in the patent application of " a kind of Vegetable oil lipoprotein Europe Lee's oil and extracting method and application ", the utilization of the by product oil meal that Europe Lee oil is produced will increase a series of product innovations for Europe Lee's sand industry.The total protein of measuring in Lee's oil meal of Europe with Kjeldahl determination reaches 34%, and is higher 6 percentage points than the total protein in the almond, has considerable utility value.

Summary of the invention

The purpose of this invention is to provide a kind of new vegetable-protein.

Vegetable-protein provided by the present invention extracts from Ou Liren.

Amino acid in the described albumen is formed and ratio of weight and number is:

?Asp	?3.5-3.9	?Ile	?1.1-1.7
				?Thr	?0.6-1.0	?Leu	?2.1-2.7
?Ser	?0.9-1.4	?Tye	?0.6-1.1
				?Glu	?10.0-11	?Phe	?1.4-2.0
?Gly	?1.8-2.2	?Lys	?0.6-1.2
				?Ala	?1.2-1.6	?His	?0.4-0.9

?Cys	?0.2-0.5	?Trp	?0.06-0.12
				?Val	?1.3-1.9	?Arg	?3.2-3.8
?Met	?0.1-0.5	?Pro	?1.0-1.6

Wherein more general ratio of weight and number is:

?Asp	?3.73	?Ile	?1.34
				?Thr	?0.87	?Leu	?2.44
?Ser	?1.13	?Tye	?0.88
				?Glu	?10.43	?Phe	?1.72
?Gly	?2.00	?Lys	?0.93
				?Ala	?1.47	?His	?0.71
?Cys	?0.38	?Trp	?0.09
				?Val	?1.59	?Arg	?3.58
?Met	?0.29	?Pro	?1.31

Protein of the present invention is made up of 18 seed amino acids, temperature of protein denaturation about 120 ℃, water-soluble protein content about 45%, the proteic solvability of Europe Li Ren higher (as shown in Figure 1) when pH4-6.Ou Liren albumen mainly is made of 14 kinds of subunits, and molecular weight subunit is (its SDS-PAGE electrophoretogram as shown in Figure 2) between 1-6 ten thousand dalton.The activity that records its SOD according to the test specification of SOD test kit (Nanjing build up biological reagent company sell) is 604.04NU (nitrite unit).

It is a lot of to produce the proteic method of Ou Liren, and method of separating protein all can use in the prior art, as adopting milling process, pressing lixiviation process, leaching method, two-phase extraction process, supercritical extraction etc. in advance.Wherein more preferably method may further comprise the steps:

1) with Ou Liren oven dry, stripping clothing, pulverizing, with sherwood oil with 1: (W: V) mixes, reflux in 40 ℃ of-60 ℃ of water-baths and stir extractions and fall grease, filtration under diminished pressure obtains degreasing Europe Li Ren to 3-5;

2) with 4-6 times of water (W: V) disperse degreasing Europe Li Ren, at room temperature with NaOH solution pH transferred to 9.0-10 while stirring, extracted 15-45 minute, centrifugal, the reservation supernatant liquor;

3) adjusting pH with HCl solution is 3.5-4.5, is settled out albumen;

4) centrifugal, precipitation is disperseed and regulated pH with distilled water is 6.5-7.5;

5) spraying drying obtains the Ou Liren protein powder.

Describedly with NaOH solution pH is transferred to 9.0-10 and should in 15 minutes, finish.Transfer the NaOH of pH and the solution that HCl is 1N.

The proteic source of Europe Li Ren of the present invention can be wild Europe Lee's a kernel, also can be Europe Lee's kernel of domestication, cultivation.Because Europe Lee's planting cost is low, and is bigger as ecological forest grass planting area, make Europe Lee's oil prodution industry production and application possess the raw material basis, thereby as the byproduct of Europe Lee's oil prodution industry, the industrialized raw material of Ou Liren albumen also is guaranteed thereupon.Utilization to Europe Lee's kernel, desertification land is administered with the economic worth of exploitation psammophyte obtained perfect the combination, the economic worth for excavating psammophyte combines ecological construction and industry development, with sand control cure sand, have important practical significance and economic implications.Europe of the present invention Li Ren albumen and active ingredient extract thereof will be used widely in fields such as feed, food, beauty and skin care goods, household chemicals, products and health products additive agent, drink additives.

The invention will be further described below in conjunction with specific embodiment.

Description of drawings

Fig. 1 is the influence curve figure of pH value to the Ou Liren protein solubility.

Fig. 2 is a Ou Liren protein SDS-PAGE electrophoretogram.

Embodiment

Embodiment 1, preparation Ou Liren albumen

1, Ou Liren oven dry, stripping clothing, pulverizing, with sherwood oil with 1: 4 (W: V) mix;

2, stir the extraction grease in 50 ℃ of water-baths backflows, filtration under diminished pressure gets degreasing Europe Li Ren;

The main composition (%) of table 1, degreasing Europe Li Ren

Moisture ash content protein content crude fat other

3.9?????????3.0??????????88.0?????????1.6??????3.5

3, degreasing Europe Li Ren is with 5 times of water-dispersions, at room temperature in 15 minutes pH transferred to 9.5 with the NaOH solution of 1N while stirring;

4, stir extraction 30 minutes, pH remains at 9.5 in whole leaching process, with 4000R/ minute centrifugal 20 minutes, removes insolubles then;

5, get supernatant liquor, adjusting pH with the HCl solution of 1N is 4, is settled out albumen;

6, centrifugal 20 minutes under the room temperature with 4000R/ minute;

7, precipitation is disperseed with distilled water and adjusting pH=7;

8, carry out spraying drying with spray-dryer, obtain Ou Liren albumen, store down for 5 ℃.

Record amino acid whose consisting of in the Li Ren albumen of resulting Europe with the amino acid tester: (unit: g/100g)

?Asp	?3.73	?Ile	?1.34
				?Thr	?0.87	?Leu	?2.44
?Ser	?1.13	?Tye	?0.88
				?Glu	?10.43	?Phe	?1.72
?Gly	?2.00	?Lys	?0.93
				?Ala	?1.47	?His	?0.71
?Cys	?0.38	?Trp	?0.09
				?Val	?1.59	?Arg	?3.58
?Met	?0.29	?Pro	?1.31

Proteic emulsifying property of embodiment 2, Ou Liren and emulsifying stability

Measuring method: with a certain amount of protein dissolution in 50ml distilled water, be mixed with certain density liquid, transfer pH to certain value, add the 50ml soybean salad oil, in mulser, spare matter 2Min with 10000R/Min, move the 10ml emulsification in centrifuge tube, centrifugal 10Min under the rotating speed of 1500R/Min, according to emulsion layer high computational emulsifying property:

Emulsion layer height (cm)/total height (cm) * 100%

The 10ml emulsification is packed in the centrifuge tube, through 80 ℃ of heating in water bath 30Min, again with the centrifugal 10Min of 1500R/Min, measure emulsion layer and separating layer height after centrifugal, calculate emulsifying stability, calculation formula is the same.

Experimental result is shown in table 2, table 3:

Table 2, Ou Liren protein concentration influence emulsifying property

Concentration	Emulsifying property	Emulsifying stability
			?0.1％	38	?36
?0.4％	43	?40
			?0.9％	58	?53
?2％	58	?52
			?3％	60	?56

Data from table 2 as can be seen, proteic emulsifying property of Ou Liren and emulsifying stability strengthen with the increase of concentration.

Table 3, pH value influence Ou Liren albumin milk voltinism

PH value	Emulsifying property	Emulsifying stability
			?2.5	?45	?43
?3.5	?41	?40
			?4.5	?46	?45
?5.5	?50	?47
			?8.5	?44	?42
?9.5	?43	?42
			?10.5	?45	?43
?11.5	?46	?44

From the data of table 3 as can be seen, proteic emulsifying property of Europe Li Ren and emulsifying stability are the highest when pH4-6.

Proteic whipability of embodiment 3, Ou Liren and froth stability

Method: a certain amount of protein is dissolved in the 100ml distilled water, regulates the pH value, in the tissue mashing machine of 10000R/Min, spare matter 2Min then to certain value, the lather volume when the even matter of record stops, the calculation formula of whipability is as follows:

Lather volume when homogeneous stops (ml)/100 (ml) * 100%

The even matter of record stops the lather volume behind 10min, 30min, 60min, the 90min, measures froth stability.Experimental result is shown in table 4, table 5:

Table 4, protein concentration influence Ou Liren albumen whipability

Concentration	????0min	??10min	??30min	??60min	??90min
						????1％	????34	????23	????21	????19	????17
????3％	????57	????45	????42	????40	????37

Data from table 4 as can be seen, when concentration increased, whipability strengthened.

Table 5, pH influence Ou Liren albumen whipability

??pH	????0min	????10min	????30min	????60min	????90min
						????9	????34	????29	????26	????25	????21
????5	????42	????15	????4	???----	????----

Bigger when from the data of table 5 as can be seen, acid to the influence of Ou Liren albumen whipability.

Claims (10)

1, a kind of xylophyta albumen extracts from Ou Liren.

2, vegetable-protein according to claim 1 is characterized in that: the amino acid in the described albumen is formed and ratio of weight and number is: ?Asp ?3.5-3.9 ?Ile ?1.1-1.7 ?Thr ?0.6-1.0 ?Leu ?2.1-2.7 ?Ser ?0.9-1.4 ?Tye ?0.6-1.1 ?Glu ?10.0-11 ?Phe ?1.4-2.0 ?Gly ?1.8-2.2 ?Lys ?0.6-1.2 ?Ala ?1.2-1.6 ?His ?0.4-0.9 ?Cys ?0.2-0.5 ?Trp ?0.06-0.12 ?Val ?1.3-1.9 ?Arg ?3.2-3.8 ?Met ?0.1-0.5 ?Pro ?1.0-1.6

3, vegetable-protein according to claim 2 is characterized in that: the amino acid in the described albumen is formed and ratio of weight and number is: ?Asp ?3.73 ?Ile ?1.34 ?Thr ?0.87 ?Leu ?2.44 ?Ser ?1.13 ?Tye ?0.88 ?Glu ?10.43 ?Phe ?1.72 ?Gly ?2.00 ?Lys ?0.93 ?Ala ?1.47 ?His ?0.71 ?Cys ?0.38 ?Trp ?0.09 ?Val ?1.59 ?Arg ?3.58 ?Met ?0.29 ?Pro ?1.31

4, the method for the described vegetable-protein of a kind of production claim 1 may further comprise the steps:

1) with Ou Liren oven dry, stripping clothing, pulverizing, with sherwood oil with 1: (W: V) mixes, reflux in 40 ℃ of-60 ℃ of water-baths and stir extractions and fall grease, filtration under diminished pressure obtains degreasing Europe Li Ren to 3-5;

2) with 4-6 times of water (W: V) disperse degreasing Europe Li Ren, at room temperature with NaOH solution pH transferred to 9.0-10 while stirring, extracted 15-45 minute, centrifugal, the reservation supernatant liquor;

3) adjusting pH with HCl solution is 3.5-4.5, is settled out albumen;

4) centrifugal, precipitation is disperseed and regulated pH with distilled water is 6.5-7.5;

5) spraying drying obtains the Ou Liren protein powder.

5, method according to claim 4 is characterized in that: describedly with NaOH solution pH is transferred to 9.0-10 and finished in 15 minutes.

6, transfer the NaOH of pH and the solution that HCl is 1N.

7, the described vegetable-protein of claim 1-3 is as the application of feed.

8, the described vegetable-protein of claim 1-3 is as the application of food.

9, the application of the described vegetable-protein of claim 1-3 in producing the beauty and skin care product.

10, the described vegetable-protein of claim 1-3 is as the application of healthcare products and medicated premix.

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