CN1454465A - Chinese yew sprout tissue culture medium and its sprout breeding method - Google Patents
Chinese yew sprout tissue culture medium and its sprout breeding method Download PDFInfo
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- CN1454465A CN1454465A CN 03117639 CN03117639A CN1454465A CN 1454465 A CN1454465 A CN 1454465A CN 03117639 CN03117639 CN 03117639 CN 03117639 A CN03117639 A CN 03117639A CN 1454465 A CN1454465 A CN 1454465A
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- chinese yew
- culture medium
- tissue culture
- tcm
- yew seedling
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Abstract
The invention discloses a Chinese yew-seedling tissue culture medium and the breeding method, made up of nutritious agent, growth promoter, stabilizing agent and microelement. It mechanically crushes tender spike branches or tender branches (buds) and leaves of the parent-body tree, then adds their crushed histiocytic plasm into the culture medium for cultivating in groups. It has simple preparing process, low cost, little loss, short cultivating time, etc. The cultivating time of the spike branches 7-15 days, and that of the histiocytic plasm 10-25 days.
Description
Technical field:
The present invention relates to a kind of medium and mating system that is used for plant.
Technical background:
Taxus world rare tree species, natural resources are few, after finding wherein to contain efficiency natural cancer therapy drug-taxol the eighties in last century, caused people's extensive concern.But because the reproductive characteristic of Chinese yew tissue culture, general conventional method is difficult to prove effective, admittedly yet there are no the document record that artificial organ is cultivated and the highly efficient regeneration plant breeds.As adopt traditional seed law or cuttage, and because of being subjected to the restriction of seedling resource, germination rate and survival rate are low in addition, and vegetative period is slow, can't form industrialization production.
Summary of the invention:
The objective of the invention is to: provide that a kind of component is simple, cost is low, enforcement is easy, expend Chinese yew parent resource few and tissue culture medium (TCM) and efficient cultivating method that breeding amount is big, to address the above problem.
The present invention is achieved in that medium is by nutritional agents, growth promoter, stabilizing agent, micro-formulated, percentage meter by measure, each component accounting example is: nutritional agents: 77.5～96.95%, growth promoter: 1～10%, stabilizing agent: 2～12%, trace element: 0.05～0.5%.
Described nutritional agents is MS or N8 standard medium.
Described growth promoter is the ethanolic solution that contains 2% gibberellin.
Described stabilizing agent is the body of paste that contains 20% agar or gelatin.
Described trace element is papain and griseofulvin.
Its mating system: with life in 1 year with give birth to then semi-lignified or not lignification Chinese yew parent-body tree length add in the above-mentioned medium at the tender pruned twig of 0.2～2cm, illumination 10～14 hours keeps 24 ± 4 ℃ of temperature, humidity 75～90% is organized training.
Its another mating system: with not lignified spray (bud) leaf, the histocyte slurry after mechanical crushing joins in the above-mentioned medium, and illumination 10～14 hours keeps 24 ± 4 ℃ of temperature, and humidity 75～90% is organized training.
The present invention compared with prior art, it is simple and practical to have a component, preparing process is easy, cost is low, consume is few, incubation time is short, can realize the advantages such as batch production production of seedling.Its skewer branch incubation time is 7～15 days, and cellular tissure slurry incubation time is 10～25 days.
Embodiment:
Embodiments of the invention 1: get nutritional agents: " MS " medium 90%, growth promoter: gibberellin+ethanol 1.9%, stabilizing agent: gelatin 8%, trace element: papain and griseofulvin 0.1%, make medium after the mixing; Add the skewer body that the Chinese yew elite stand spray (with 1～2 tender shoots) of giving birth to semi-lignified then is about 0.6cm again, 24 ± 4 ℃ of environmental temperature temperature, humidity 80～85%, intensity of illumination 1850LX, illumination every day 11 hours, can produce root system about 10 days, form regeneration plant, its batch survival rate is 83%.
Embodiment 2: get nutritional agents: " N8 " medium 88.5%, produce promoter: and gibberellin+ethanol 2%, stabilizing agent: gelatin+agar 9%, trace element: papain and griseofulvin 0.5%, make medium after the mixing; Add life in 1 year or the slurry of the histocyte after the pulp of lignification Chinese yew spray (bud) leaf mechanical crushing not then, illumination every day 14 hours (after 5 days 10 hours), intensity of illumination 1900LX (2000XL after 5 days), 24 ± 4 ℃ of environmental temperatures, humidity 84～88%, can form single young seedling in 15～25 days, can be implanted in the culture tube (2 times grow seedlings) after 25 days, survival rate reaches 30%～40% in batches.
Claims (7)
1, a kind of Chinese yew seedling tissue culture medium (TCM), it is by nutritional agents, growth promoter, stabilizing agent, micro-formulated, it is characterized in that: percentage meter by measure, each component accounting example is: nutritional agents: 77.5～96.95%, growth promoter: 1～10%, stabilizing agent: 2～12%, trace element: 0.05～0.5%.
2, Chinese yew seedling tissue culture medium (TCM) according to claim 1 is characterized in that: described nutritional agents is MS or N8 standard medium.
3, Chinese yew seedling tissue culture medium (TCM) according to claim 1, it is characterized in that: described growth promoter is the ethanolic solution that contains 2% gibberellin.
4, Chinese yew seedling tissue culture medium (TCM) according to claim 1, it is characterized in that: described stabilizing agent is the body of paste that contains 20% agar or gelatin.
5, Chinese yew seedling tissue culture medium (TCM) according to claim 1, it is characterized in that: described trace element is papain and griseofulvin.
6, a kind of mating system of Chinese yew seedling, it is characterized in that: with life in 1 year with give birth to then semi-lignified or not lignification Chinese yew parent-body tree length add in the above-mentioned medium at the tender pruned twig of 0.2～2cm, illumination 10～14 hours keeps 24 ± 4 ℃ of temperature, humidity 75～90% is organized training.
7, a kind of mating system of Chinese yew seedling is characterized in that: also available not lignified spray (bud) leaf, and the histocyte slurry after mechanical crushing joins in the above-mentioned medium, illumination 10～14 hours, keep 24 ± 4 ℃ of temperature, humidity 75～90% is organized training.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031176399A CN100453639C (en) | 2003-04-04 | 2003-04-04 | Chinese yew sprout tissue culture medium and its sprout breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031176399A CN100453639C (en) | 2003-04-04 | 2003-04-04 | Chinese yew sprout tissue culture medium and its sprout breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1454465A true CN1454465A (en) | 2003-11-12 |
| CN100453639C CN100453639C (en) | 2009-01-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031176399A Expired - Fee Related CN100453639C (en) | 2003-04-04 | 2003-04-04 | Chinese yew sprout tissue culture medium and its sprout breeding method |
Country Status (1)
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| CN (1) | CN100453639C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630566A (en) * | 2012-04-20 | 2012-08-15 | 吉首大学 | Method for cultivation and production of paclitaxel from induction of taxus chinensis var. mairei axillary bud in vitro |
| CN102640710A (en) * | 2012-05-21 | 2012-08-22 | 上海应用技术学院 | Method for induction and subculture of taxus chinensis leaf callus and culture medium used by method |
| CN102657091A (en) * | 2012-05-21 | 2012-09-12 | 上海应用技术学院 | Taxus chinensis var. maire embryo in-vitro seedling forming technique and culture medium employed by same |
| CN103039365A (en) * | 2013-01-18 | 2013-04-17 | 通化师范学院 | Test tube one-step seedling method of taxus cuspidata |
| CN106818387A (en) * | 2017-03-24 | 2017-06-13 | 江苏红豆杉生物科技股份有限公司 | A kind of method that utilization Water moss ball plants Taxus x media |
| CN109287482A (en) * | 2018-10-30 | 2019-02-01 | 江西智汇中药材种植有限公司 | A kind of Yellow Fructus Gardeniae tissue-culturing rapid propagation special culture media |
-
2003
- 2003-04-04 CN CNB031176399A patent/CN100453639C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630566A (en) * | 2012-04-20 | 2012-08-15 | 吉首大学 | Method for cultivation and production of paclitaxel from induction of taxus chinensis var. mairei axillary bud in vitro |
| CN102640710A (en) * | 2012-05-21 | 2012-08-22 | 上海应用技术学院 | Method for induction and subculture of taxus chinensis leaf callus and culture medium used by method |
| CN102657091A (en) * | 2012-05-21 | 2012-09-12 | 上海应用技术学院 | Taxus chinensis var. maire embryo in-vitro seedling forming technique and culture medium employed by same |
| CN103039365A (en) * | 2013-01-18 | 2013-04-17 | 通化师范学院 | Test tube one-step seedling method of taxus cuspidata |
| CN106818387A (en) * | 2017-03-24 | 2017-06-13 | 江苏红豆杉生物科技股份有限公司 | A kind of method that utilization Water moss ball plants Taxus x media |
| CN109287482A (en) * | 2018-10-30 | 2019-02-01 | 江西智汇中药材种植有限公司 | A kind of Yellow Fructus Gardeniae tissue-culturing rapid propagation special culture media |
| CN109287482B (en) * | 2018-10-30 | 2022-03-01 | 江西智汇中药材种植有限公司 | Special culture medium for tissue culture and rapid propagation of gardenia jasminoides |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100453639C (en) | 2009-01-21 |
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Granted publication date: 20090121 Termination date: 20110404 |