CN1429478A - Production technology of hybridized rice seed - Google Patents
Production technology of hybridized rice seed Download PDFInfo
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- CN1429478A CN1429478A CN 01138368 CN01138368A CN1429478A CN 1429478 A CN1429478 A CN 1429478A CN 01138368 CN01138368 CN 01138368 CN 01138368 A CN01138368 A CN 01138368A CN 1429478 A CN1429478 A CN 1429478A
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Abstract
A production technique for breeding the seeds of hybridied rice includes such steps as choosing seeds of the herbicide resistant sterile line A and the herbicide-resistant maintenance line B, crossing to obtain the seeds of male sterile line A, breeding herbicide-lethal maintenance line B' from B, hybridizing A with B' to obtain the seeds of male sterile line A', using herbicide to kill the plant of B' after pollination, and hybridization between the seeds of male sterile line A' and maintenance line. The maintenance line lethal-sensitive to bentazon can be used to implement the said production technique. Its advantages are simple process, high productivity and high purity of seed.
Description
The invention belongs to a kind of production technology of hybridized rice seed, especially produce the technology of Three-line Hybrid rice paddy seed by chemicals treatment.
Existing correlation technique about this invention is described below:
1. the Three-line Hybrid paddy rice utilizes the paddy rice cytoplasmic-nuclear male sterility to carry out seed production.The seed that is used for the maternal male sterile line of hybrid seeding is to obtain behind the outbreeding by male sterile line and maintenance line, and maintenance line then passes through self propagated.In existing production technology, maintenance line and male sterile line must branch be cultivated, the ripe back of pollination harvesting dividedly.This method is labor intensive not only, and because maintenance line is the same with the male sterile line form, is easy to cause the mechanical admixture of seed.Be used for hybrid seeding if will be mixed with the male-sterile seed of maintenance line seed, maintenance line plant loose powder in seed farm will be used in the seed that is mixed with male sterile line and maintenance line in the hybrid rice seeds that the land for growing field crops produces.This specific admixture is the main cause that hybrid rice seeds mixes, and causes production of hybrid seeds failure or production loss when serious.
2. Bentazon is a kind of rice field time face herbicide spraying commonly used.Common rice is anti-Bentazon under common consumption (0.5～1.5 kilogram of per hectare), but the Bentazon of same consumption can effectively kill broad-leaved class and sedge weed.Mori.K in 1984 has reported by the agricultural of radioinduction japonica rice variety and has obtained mutant (Jpa.J.Breed.1984 to the Bentazon sensitive lethality for No. 8,34 (suppl.): 421-422), Zhang Jiwen in 1997 etc. have also obtained mutant (rice in China science to the Bentazon sensitive lethality by radioinduction photo-thermo-sensitive genic male sterile rice line W6154S, 1999,13 (2); 65-68).Zhang Jiwen etc. studies confirm that, sudden change is controlled by a recessive single-gene paddy rice to the Bentazon sensitive lethality, derive from the equipotential sudden change that agricultural are same sites with two mutant that derive from W6154S No. 8, this mutator gene is named as bel., and is located near the microsatellite DNA mark RM55 on the trisome; Paddy rice to Bentazon sensitive lethality mutant simultaneously also to the sulfonylurea herbicide sensitivity, but other economical character to paddy rice does not have tangible negative effect, can change other rice varieties easily over to by method such as backcross, be an important genetic resources that can be used for the hybrid rice parent is carried out genetic marker; Use the method for backcross transformation or induced mutations, can directionally an anti-Bentazon rice varieties be cultivated paired Bentazon sensitive lethality and other economical characters and constant substantially new varieties (hybrid rice, 2000,15 (6): 5-8) of former kind maintenance.
3. use paddy rice Bentazon sensitive lethality mutant character and disclose two kinds of important technologies that are used for hybrid rice seeds production.A kind of be the intermingle method seeding technique discussed such as Maruyama K. with Bentazon sensitive lethality mutant character introductive crossing rice restorer (Japan Agricultural Research Quarterly.1991,24:4,243-252).Its technical scheme is to be seed mixed seeding by a certain percentage with male-sterile seed and recovery with Bentazon sensitive lethality mark, after recovering to be the plant blossom loose powder, spray Bentazon solution, killing recovery is plant, keep the male sterile line plant, so that the mechanized harvest of made hybrid seed.Thereafter, (hybrid rice, 1992 such as Wang Zhong, 6:40-41), Chen Zhongming etc. (hybrid rice, 1999,2:39-40) this method has been discussed also, Zhu Qisheng etc. (hybrid rice, 2000,15 (3) :) have also carried out the work that transformation has the recovery system of Bentazon sensitive lethality mutant character.This technical essential is with Bentazon sensitive lethality mutant character introductive crossing rice restorer.The main difficult point that realizes this technology is that the breeding time of requirement recovery system and male sterile line is very close, and the existing hybrid combination of promoting can not be satisfied this requirement, thereby this technology imagination does not obtain practical application.Chinese patent " a kind of method of cultivating hybrid rice seeds " (application number 88109218) has required intermingle law technology patent but has abandoned.
4. the another kind of important technology of disclosed application paddy rice Bentazon sensitive lethality mutant character is that the application Bentazon sensitive lethality mark by inventions such as Zhang Jiwen carries out the pure technology of hybrid rice removal of impurities guarantor.This technology discloses and has applied for multinomial patent.Publication number is that the patent application of CN1217870A is carried out the production of hybrid seeds with Bentazon sensitive lethality mutant character introductive crossing paddy rice female parent, after planting sprays Bentazon at hybrid seed, kills maternal self-crossing seedling.Application number is maintenance line and the male sterile line that 00114492.8 patent application imports Bentazon sensitive lethality mutant character the Three-line Hybrid paddy rice simultaneously, application is to the maintenance line and the same male sterile line to the Bentazon sensitive lethality of Bentazon sensitive lethality, outbreeding produces the male-sterile seed to the Bentazon sensitive lethality, with this male-sterile seed and recovery to the Bentazon sensitive lethality is that hybrid seed is produced in the production of hybrid seeds, after planting spray Bentazon at hybrid seed, kill maintenance line and the male sterile line seedling that to sneak into, reach removal of impurities and protect pure purpose.This method requires maintenance line and male sterile line to have simultaneously the recessive homozygous genotype of Bentazon sensitive lethality.
5. employing transgenic technology can have been cultivated the rice varieties of not anti-certain weed killer herbicide and become to resist this kind weed killer herbicide to keep the new varieties of former kind economical character simultaneously.Transgenic technology that rice in China adopts imports the capital with the Bar. gene and draws in 119 rice varieties such as grade, and (Science Bulletin 1,998 43 (1): 67-70), its resistance is the dominance monogenic inheritance to have obtained the transgenic paddy rice of antiweed grass fourth phosphine.
The present invention is the new technological invention based on above-mentioned public technology.
The objective of the invention is to protect pure a kind of more simple and effective technology that provides for seed production and the seed of hybrid rice.
The object of the present invention is achieved like this:
A kind of Three-line Hybrid rice paddy seed production method, this method comprises: the seed that 1. adopts the maintenance line B outbreeding male sterile line A of the male sterile line A of antiweed and antiweed: 2. maintenance line B is cultivated the homotype maintenance line B ' that paired herbicide sensitive causes death, adopt male sterile line A and maintenance line B ' outbreeding to produce the seed of male sterile line A ' then, after maintenance line B ' is bloomed pollination, use weed killer herbicide breeding Tanaka and kill its plant; 3. the seed of the male sterile line A ' that is gathered in the crops is used for and recovery is that hybrid rice seeds is produced in the production of hybrid seeds.
Described weed killer herbicide is a Bentazon.
Described weed killer herbicide is a sulfonylurea herbicide.
Described male sterile line is that cytoplasmic sterile line or short cytoplasmic sterile line or ridge type cytoplasmic sterile line or D type cytoplasmic sterile line or red lotus type cytoplasmic sterile line or seal water type cytoplasmic sterile line or Yunnan type cytoplasmic sterile line or the BT type cytoplasmic sterile line of losing are lost in the open country.
Described male sterile line is precious Xian 97A or V20A or golden 23A or II-32A or Long Tepu A or Gang No.46A or D62A or D297A or D Xian A or Xieqingzao A or K17A.
Below technique scheme and technical term are further explained:
The Three-line Hybrid paddy rice is to use the cytoplasmic-nuclear male sterility principle to carry out seed production.For a kind of cytoplasmic-nuclear male sterility A, corresponding maintenance line B with dyskaryosis must be arranged.To the classification of cytoplasmic nuclear male sterile line, can be divided into the open country according to its cytoplasmic difference and lose cytoplasmic sterile line, the short cytoplasmic sterile line that loses, ridge type cytoplasmic sterile line, D type cytoplasmic sterile line, red lotus type cytoplasmic sterile line, seal water type cytoplasmic sterile line, Yunnan type cytoplasmic sterile line, BT type cytoplasmic sterile line or the like (referring to rice in China kind and pedigree thereof, Shanghai science tech publishing house, in March, 1991 front page, the 190-196 page or leaf, the 209-210 page or leaf).In the technical scheme of the present invention, except the maintenance line B of the same dyskaryosis of the male sterility A that uses antiweed and corresponding antiweed thereof, also by backcross transformation or induced mutations or transgenic technology, with antiweed maintenance line B cultivate the new maintenance line B ' that paired this herbicide sensitive causes death.Maintenance line B ' recessiveness on the gene loci that this herbicide sensitive is caused death is isozygotied, it and former maintenance line B are except sample that this weed killer herbicide is had various reactions, all the same substantially on other economical characters, male sterile line A had identical confining force, to recovering cording the same coordinate force with former maintenance line B is arranged, we are called " homotype maintenance line " with maintenance line B ' and maintenance line B.For example, by backcross transformation or induced mutations, with the maintenance line B of anti-Bentazon
1Cultivate the new maintenance line B of paired Bentazon sensitive lethality
1', make maintenance line B
1' (the rice in China science, 1999,13 (2): 65-68) described Bentazon sensitive lethality proterties, recessiveness is isozygotied on the bel. gene loci to have document.It and former maintenance line B
1Except sample that Bentazon is had various reactions, all the same substantially on other economical characters, to male sterile line A
1Have identical confining force, have and former maintenance line B recovering cording
1The same coordinate force, we are with maintenance line B
1' and maintenance line B
1Be called " homotype maintenance line ".Again for example, incite somebody to action the not maintenance line B of antiweed " careless fourth phosphine " by transgenic technology
2' bred the maintenance line B of dominance antiweed " careless fourth phosphine "
2, maintenance line B
2' and maintenance line B
2Have identical confining force and coordinate force, it also is known as " homotype maintenance line ".
In the Three-line Hybrid paddy rice, male sterile line and maintenance line have very close breeding time and morphological characters, therefore can satisfy the specification requirement of the intermingle method production of hybrid seeds.The seed of male sterile line A and maintenance line B ' is mixed the back seeding and seedling raising by a certain percentage, mix and transplant, pollination back spraying herbicide kills maintenance line B ' plant, the male sterile line A ' seed that ripe back results are bred.Because the male sterile line A ' seed of being bred is the herbicide sensitive gene heterozygosis, and antiweed is a dominant inheritance, thereby male sterile line A ' is insensitive to weed killer herbicide, even be mixed with the seed of maintenance line B ', also can in seed farm, with medicament dispose, guarantee the hybrid rice seeds purity that use in the land for growing field crops.In the breeding of some male sterile line, require maintenance line and male sterile line that poor phase of broadcasting about 3 days is arranged, at this moment male sterile line and maintenance line seed can be separated vernalization, mix then or the gradation sowing, still can adopt the breeding of intermingle method.
Advantage of the present invention and good effect are as follows:
1. use this technology and can effectively eradicate maintenance line seed mechanical admixture in the sterile line propagation process, thereby improve the purity of hybrid rice seeds.
2. use this technology, adopt intermingle method breeding male sterile lines seed, simplified the planting patterns in breeding field, can carry out mechanized harvest, improve the labor productivity of sterile line propagation, improve the results quality of seed.
Embodiments of the invention are as follows:
II-32A is a good seal water type kytoplasm cytoplasmic nuclear male sterile line, and its maintenance is II-32B.The maternal male-sterile seed that is used for hybrid seeding is produced with II-32A * II-32B outbreeding.1998-2001 adopts disclosed mutant 8077 hybridization among II-32B and the CN1217870A, and is that recurrent parent is repeatedly backcrossed with II-32B, breeds the homotype maintenance line II-32B ' with Bentazon sensitive lethality mark.With II-32A * II-32B breeding II-32A seed.Use II-32A * II-32B ' outbreeding again, just can obtain to be used for the maternal male sterile line II-32A ' seed of preparing hybrid combination seed.With II-32A ' * recovery is 501, seed that just can production hybrid combination II excellent 501.In production process with II-32A * II-32B ' outbreeding II-32A ' seed, ratio according to 10: 1, seed mixed seeding with II-32A and II-32B ', mixing is grown seedlings, mix to transplant, after II-32B ' is bloomed, manually catch up with powder to finish after, every mu sprays about 100 liters of 0.1% Bentazon solution, can kill II-32B ' plant, and the plant of male sterile line II-32A is safe and harmless.After treating the hybrid seed maturation on the male sterile line II-32A, just can be manually or the pure seed of mechanized harvest male sterile line II-32A '.This II-32A ' seed has the same using value with the II-32A seed, but can guarantee wherein can not be mixed with the maintenance line seed, thereby guarantees the purity of the seed of the hybrid combination II excellent 501 that makes.
Claims (5)
1. Three-line Hybrid rice paddy seed production method is characterized in that this method comprises: the seed that 1. adopts the maintenance line B outbreeding male sterile line A of the male sterile line A of antiweed and antiweed; 2. maintenance line B is cultivated the homotype maintenance line B ' that paired herbicide sensitive causes death, adopt male sterile line A and maintenance line B ' outbreeding to produce the seed of male sterile line A ' then, after maintenance line B ' is bloomed pollination, use weed killer herbicide breeding Tanaka and kill its plant; 3. the seed of the male sterile line A ' that is gathered in the crops is used for and recovery is that hybrid rice seeds is produced in the production of hybrid seeds.
2. as claim 1 described method, it is characterized in that described weed killer herbicide is a Bentazon.
3. as claim 1 described method, it is characterized in that described weed killer herbicide is a sulfonylurea herbicide.
4. as claim 1 described method, it is characterized in that described male sterile line is that cytoplasmic sterile line or short cytoplasmic sterile line or ridge type cytoplasmic sterile line or D type cytoplasmic sterile line or red lotus type cytoplasmic sterile line or seal water type cytoplasmic sterile line or Yunnan type cytoplasmic sterile line or the BT type cytoplasmic sterile line of losing are lost in the open country.
5. as claim 1 described method, it is characterized in that described male sterile line is precious Xian 97A or V20A or golden 23A or II-32A or Long Tepu A or Gang No.46A or D62A or D297A or D Xian A or Xieqingzao A or K17A.
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|---|---|---|---|
| CN 01138368 CN1429478A (en) | 2001-12-29 | 2001-12-29 | Production technology of hybridized rice seed |
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| CN 01138368 CN1429478A (en) | 2001-12-29 | 2001-12-29 | Production technology of hybridized rice seed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342243A (en) * | 2010-07-28 | 2012-02-08 | 安徽省农业科学院水稻研究所 | Production method for mechanized production of rice seed |
| CN101766115B (en) * | 2009-12-28 | 2013-03-13 | 湖北大学 | Culturing rice near-isogenic restoring line by utilizing antiphyte-type sterile cytoplasm and maintainer line |
| CN105104166A (en) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | Method used for breeding imidazolinone herbicide-resistant japonica rice |
| CN107155873A (en) * | 2017-07-17 | 2017-09-15 | 安徽省农业科学院水稻研究所 | One kind makes the acarpous producing method for seed of hybrid paddy rice male parent |
-
2001
- 2001-12-29 CN CN 01138368 patent/CN1429478A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766115B (en) * | 2009-12-28 | 2013-03-13 | 湖北大学 | Culturing rice near-isogenic restoring line by utilizing antiphyte-type sterile cytoplasm and maintainer line |
| CN102342243A (en) * | 2010-07-28 | 2012-02-08 | 安徽省农业科学院水稻研究所 | Production method for mechanized production of rice seed |
| CN105104166A (en) * | 2015-07-23 | 2015-12-02 | 上海市农业科学院 | Method used for breeding imidazolinone herbicide-resistant japonica rice |
| CN105104166B (en) * | 2015-07-23 | 2021-05-07 | 上海市农业科学院 | Method for breeding imidazolinone herbicide-resistant japonica rice |
| CN107155873A (en) * | 2017-07-17 | 2017-09-15 | 安徽省农业科学院水稻研究所 | One kind makes the acarpous producing method for seed of hybrid paddy rice male parent |
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