CN1387034A - Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing - Google Patents
Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing Download PDFInfo
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- CN1387034A CN1387034A CN 02115395 CN02115395A CN1387034A CN 1387034 A CN1387034 A CN 1387034A CN 02115395 CN02115395 CN 02115395 CN 02115395 A CN02115395 A CN 02115395A CN 1387034 A CN1387034 A CN 1387034A
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Abstract
A fast measuring method and a device for the photosynthetic speed of delayed light generated by plant leaf under induction of visual light are disclosed. Said method incldues such steps as visual light induction of plant leaf for generating delayed light, converting the light to electric signal, A/D conversion, data processing to obtain light intensity, and searching a match in database to obtain its photosynthetic speed. Said device is composed of light exciting module, light receiving module, A/D converter, computer and data processing software.
Description
(1) technical field
The present invention relates to a kind of photosynthesis rate measuring technique, method and the device thereof that photosynthetic rate detects carried out in particularly a kind of delayed luminescence that utilizes visible light-inducing.
(2) background technology
In photosynthesis rate was measured, generally the instrument of Shi Yonging was a LI-62XX series photosynthetic rate analyzer at present.Its principle is according to blade CO on unit interval, unit area
2Reduction determine the photosynthetic rate of blade.There are many problems in this instrument, mainly shows the following aspects: (1) this instrument in measuring process is subjected to the restriction of external environment factor more serious.As light intensity, humidity, CO
2The change of concentration moment all can bring very big difference to measurement result.Therefore, measure one group of active data and often will spend long time.(2) because when measuring, the lasting time is longer, and be engraved in change during extraneous ambient condition, therefore, the data that record are a series of random numbers, can not reflect the photosynthetic rate of tested plant under physiological condition of living at that time.(3) design limit of leaf chamber to the measurement of blade.Because the leaf chamber size is fixing, the blade blade face of measurement is excessive or too smallly all can make troubles to measurement, even brings error to measurement result.(4) since in measuring process the breathing and the transpiration of plant leaf blade, make the environmental baseline (CO that leaf is indoor
2Concentration, humidity etc.) and extraneous condition inconsistent, and instrument itself can not well compensate this difference.(5) this instrument cost is relatively more expensive, is unfavorable for the universal use in agricultural production.
(3) summary of the invention
The object of the present invention is to provide a kind of delayed luminescence to carry out the photosynthetic rate method for quick by visible light-inducing.Mainly be to use the harmless visible light of blade is carried out exciting of short time to blade, induce chloroplast to produce the delayed luminescence of long period, and utilize this delayed luminescence to characterize the photosynthetic rate of blade.
Another object of the present invention is to provide a kind of delayed luminescence photosynthetic rate device for fast detecting of realizing the visible light-inducing of said method.
Purpose of the present invention is achieved through the following technical solutions, and the delayed luminescence photosynthetic rate detection method of this visible light-inducing comprises the steps:
(1) blade is placed the darkroom, dark adatpation 10～20s;
(2) be that 2750～5500lux, wavelength coverage are the even irradiation blade of excited by visible light light source 0.2～20s of 400～760nm with light intensity, the inducer blade chloroplast produces the delayed luminescence of long period;
(3) utilize optical fiber receive module to receive this delayed luminescence signal, and convert thereof into electric signal from blade;
(4) electric signal is converted to digital signal by analog to digital converter;
(5) utilize software that digital signal is carried out data processing, obtain blade integral or local delayed luminescence intensity.
(6) in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain the photosynthetic rate of tested blade.
The wavelength of selected exciting light sources can be chosen in the wavelength of visible light scope, and the exciting light light intensity is lower than the blade injury threshold value.
Close light source after inducing chloroplast to produce the delayed luminescence of long period with the even irradiation blade of excitation source, darkening 2～10s can avoid the interference from the fluorescence of chlorophyll a like this; Because of delayed luminescence decay fast in time, shorten the darkening time, can improve the delayed luminescence signal intensity.
Above-mentioned excitation source is a light intensity when being the excitation source of 2750～5500lux, and evenly irradiation blade 0.2～20s can obtain long-term delayed luminescence signal.
The delayed luminescence photosynthetic rate pick-up unit of visible light-inducing of the present invention comprises that electrical connection between electrical connection between excited by visible light assembly, optical fiber receive module, digital to analog converter, computing machine, light excitation component and the computing machine, optical fiber receive module and the digital to analog converter, analog to digital converter are connected with signal between the computing machine.
Described optical fiber receive module is the device that is used to survey light, and is comparatively faint comparatively speaking because of photoinduction delayed luminescence, can adopt the weak light detection system, for example various photomultipliers, CCD or enhancement mode CCD imaging system (ICCD) etc.
Described analog to digital converter can adopt general analog to digital converter, or has other devices of analog-digital conversion function.The CCD controller that for example image series capture card of National Instruments company production, or Princeton Instruments company is produced.
General data-signal process software is housed in the described computing machine,, is used for blade delayed luminescence Signal Processing as the LabVIEW software of NationalInstruments company exploitation or the WINVIEW software of Princeton Instruments company exploitation.
The present invention compared with prior art has following advantage and effect:
(1) the delayed luminescence photosynthetic rate method for quick of this visible light-inducing combines the short time irradiation delayed luminescence of visible light and the advantage of optical imagery (when receiving unit is CCD or ICCD), the photosynthetic power of blade can be described from morphologic angle, the average photosynthesis rate that not only can reflect whole blade, and can also reflect the power of blade different parts photosynthetic rate, compare with traditional detection method and have more directly perceived, clear comprehensive advantage.
(2) the delayed luminescence photosynthetic rate fast detecting of this visible light-inducing is to carry out in dark situation (leaf chamber).Utilize the excitation source that carries when exciting, can fixedly excite light intensity to every group of measurement, avoided in the traditional technique in measuring process changing the error of the measurement data of bringing owing to the moment of light intensity, therefore, stable, accurate, timesaving advantage that this method has.
(3) the delayed luminescence photosynthetic rate detection method operating process of this visible light-inducing is simple, realizes easily; And the apparatus structure of realizing this method is simple, and the production assembling is more or less freely, easy for operation, and the package unit cost is relatively low, is beneficial to popularize and promote.
(4) description of drawings
Fig. 1 is a kind of device synoptic diagram of the delayed luminescence photosynthetic rate device for fast detecting of visible light-inducing of the present invention;
Fig. 2 is that peanut leaf dark adatpation 20s excites darkening 2s in the 4s darkroom to open the take pictures shutter image of camera lens of light harvesting by computer control then in concrete the enforcement;
Fig. 3 is applied to big leaf of Flos Carthami sheet photosynthetic rate detected image with above-mentioned steps.
(5) embodiment
Below in conjunction with accompanying drawing the present invention is done further concrete description, but embodiments of the present invention are not limited thereto.
Execute example 1
Fig. 1 has provided a kind of concrete structure synoptic diagram of the delayed luminescence photosynthetic rate device for fast detecting of visible light-inducing of the present invention.As seen from Figure 1, this device comprises excitation component, optical fiber receive module, analog to digital converter, computing machine, printer.Wherein optical fiber receive module is connected and composed by take pictures camera lens and the detector ICCD that collect light; Light excitation component and computing machine are electrically connected, and reception group, analog to digital converter and computing machine are electrically connected successively; Three-dimensional adjustable specimen holder is placed blade to be measured on the frame, place the darkroom.Select for use each member to connect to form this device, wherein, it is visible excitation source that excitation source is selected wavelength coverage for use, and this selects the 50W tungsten lamp for use.The camera lens of taking pictures is selected the big numerical aperture camera lens (50mm, f/1.2 type) of the Nikon company manufacturing that is applicable to that the low light level is taken for use; The ICCD-576-S/1 (enhancement mode CCD) that detector selects for use U.S. Princeton Instruments company to make; The ST-130 type controller that analog to digital converter selects for use U.S. PrincetonInstruments company to make; Computing machine is selected the Pentium III type microcomputer of Intel Company for use; Data processing software is selected WINVIEW for use.
Said apparatus is applied to the detection of the photosynthetic rate of peanut: peanut leaf is placed on the sample stage in darkroom, make its place, focal plane that is in the light harvesting camera lens, close the darkroom, dark adatpation 20s.By the shutter that is in the light of computer control excitation source, make exciting light evenly excite the blade face.Excite the be in the light shutter and the power supply of closing excitation source behind the 4s by computer control, fluorescence for fear of chlorophyll a, close being in the light soon behind the door of excitation source, allow blade darkening 2s in the darkroom, open the take pictures shutter of camera lens of light harvesting by computer control then, the delayed luminescence of record blade is carried out image weight by analog to digital converter and computing machine, obtain the image of delayed luminescence, as shown in Figure 2.The delayed luminescence of record blade is carried out image by analog to digital converter and computing machine and is weighed the delayed luminescence image that obtains.With WINVIEW software processes image, obtain blade integral or local delayed luminescence intensity, in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain corresponding to the photosynthetic rate of this luminous intensity.The average photosynthetic rate of its correspondence is 5.4ppm/scm
2With WINVIEW software processes image, obtain blade integral delayed luminescence intensity, in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain corresponding to the photosynthetic rate of this luminous intensity.The average photosynthetic rate of its correspondence is 5.4ppm/scm
2
Embodiment two
According to the aforesaid operations step, measure the photosynthetic rate of plant bright red flower.The image of rebuilding as shown in Figure 3, its corresponding average photosynthetic rate is 3.6ppm/scm
2
Claims (10)
1, a kind of delayed luminescence photosynthetic rate method for quick of visible light-inducing is characterized in that comprising the steps;
(1) plant leaf blade is placed on dark adatpation 10～20s in the leaf chamber;
(2) with excited by visible light light source activation plant leaf blade 1～4s, induce it to produce delayed luminescence;
(3) utilize light to accept assembly and accept the delayed luminescence that this comes from chloroplast, and be electric signal this delayed luminescence conversion of signals;
(4) utilize analog to digital converter that electrical signal conversion is digital signal;
(5) processing digital signal obtains the delayed luminescence intensity of blade;
(6) in delayed luminescence intensity of having set up and the corresponding database of photosynthetic rate, contrast this luminous intensity, obtain the photosynthetic rate of tested blade.
2, leaf according to claim 1 chamber is the darkroom that metal or other non-light transmissive material constitute.
3, the delayed luminescence photosynthetic rate method for quick of visible light-inducing according to claim 1 is characterized in that excitation component is that light intensity is the light source of the limit of visible spectrum of 2750～5500lux.
4, excitation light intensity 2750～5500lux according to claim 3 has satisfied the saturation point of the required exciting light of delayed luminescence.
5, the photosynthetic method for quick of the delayed luminescence of visible light-inducing according to claim 1, when it is characterized in that utilizing the excitation light source excites plant leaf blade, uniform irradiation 0.2～20s.
6, the delayed luminescence photosynthetic rate method for quick of visible light-inducing according to claim 3 is characterized in that excitation light intensity can not damage plant tissue.
7, the delayed luminescence photosynthetic rate method for quick of visible light-inducing according to claim 1, after it is characterized in that the even irradiation plant leaf blade of described excitation source, close excitation source, darkening 2～5s, the delayed luminescence of the chloroplast of opening entry visible light-inducing then.
8, the spectral range of excitation source according to claim 3 is 400～760nm, the optimum range 680～710nm of the delayed luminescence action spectrum that has comprised.
9, the delayed luminescence photosynthetic rate device for fast detecting of the visible light-inducing of the described method use of claim 1, it is characterized in that comprising that excited by visible light assembly, light are accepted assembly, analog to digital converter is electrically connected with computing machine with computing machine, light excitation component, light is accepted assembly, analog to digital converter is connected with computing machine signal successively.
10, the delayed luminescence photosynthetic rate device for fast detecting of visible light-inducing according to claim 9 is characterized in that light accepts assembly and can adopt all kinds of photomultipliers, CCD, ICCDD detection system.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB021153957A CN1168973C (en) | 2002-06-18 | 2002-06-18 | Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing |
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| CNB021153957A CN1168973C (en) | 2002-06-18 | 2002-06-18 | Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397069C (en) * | 2004-04-28 | 2008-06-25 | 华南师范大学 | Monitoring method and equipment for ecological environment and environmental pollution |
| CN100447568C (en) * | 2005-04-19 | 2008-12-31 | 华南师范大学 | Quick detector for photosynthetic capacity of irradiation from light emitting diode |
| CN1804590B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | Method for monitoring crop growth regulation and control |
| CN1804589B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | Method and apparatus for monitoring plant stress physiology and screening high-resistance plant |
| CN103234916A (en) * | 2013-04-06 | 2013-08-07 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103925992A (en) * | 2013-01-16 | 2014-07-16 | 光宝电子(广州)有限公司 | Brightness measurement method and system with backlight device |
| CN103954562A (en) * | 2014-04-09 | 2014-07-30 | 福建农林大学 | Photosynthesis machine based on optical fiber sensor |
| CN104713861A (en) * | 2015-03-17 | 2015-06-17 | 深圳市海王英特龙生物技术股份有限公司 | Dark cavity and in-vitro detection and analysis device using dark cavity |
| CN105891162A (en) * | 2016-03-30 | 2016-08-24 | 山东省花生研究所 | Method for measuring peanut photosynthetic lagged extent by constructing mathematical model |
| CN106525821A (en) * | 2016-10-31 | 2017-03-22 | 山东省医药生物技术研究中心 | Method and device for detecting medicinal property of traditional Chinese medicine |
| CN110398485A (en) * | 2018-04-24 | 2019-11-01 | 南京简智仪器设备有限公司 | A kind of detection device and detection method based on difference Raman technology |
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2002
- 2002-06-18 CN CNB021153957A patent/CN1168973C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397069C (en) * | 2004-04-28 | 2008-06-25 | 华南师范大学 | Monitoring method and equipment for ecological environment and environmental pollution |
| CN100447568C (en) * | 2005-04-19 | 2008-12-31 | 华南师范大学 | Quick detector for photosynthetic capacity of irradiation from light emitting diode |
| CN1804590B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | Method for monitoring crop growth regulation and control |
| CN1804589B (en) * | 2005-12-28 | 2010-05-12 | 华南师范大学 | Method and apparatus for monitoring plant stress physiology and screening high-resistance plant |
| CN103925992B (en) * | 2013-01-16 | 2016-03-16 | 光宝电子(广州)有限公司 | There are the brightness measurement method and system of the device of backlight |
| CN103925992A (en) * | 2013-01-16 | 2014-07-16 | 光宝电子(广州)有限公司 | Brightness measurement method and system with backlight device |
| CN103234916B (en) * | 2013-04-06 | 2015-03-18 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103234916A (en) * | 2013-04-06 | 2013-08-07 | 吉林大学 | Prediction method for net photosynthetic rate of population |
| CN103954562A (en) * | 2014-04-09 | 2014-07-30 | 福建农林大学 | Photosynthesis machine based on optical fiber sensor |
| CN104713861A (en) * | 2015-03-17 | 2015-06-17 | 深圳市海王英特龙生物技术股份有限公司 | Dark cavity and in-vitro detection and analysis device using dark cavity |
| CN105891162A (en) * | 2016-03-30 | 2016-08-24 | 山东省花生研究所 | Method for measuring peanut photosynthetic lagged extent by constructing mathematical model |
| CN105891162B (en) * | 2016-03-30 | 2018-07-03 | 山东省花生研究所 | A kind of method for measuring the photosynthetic delay degree of peanut by building mathematical model |
| CN106525821A (en) * | 2016-10-31 | 2017-03-22 | 山东省医药生物技术研究中心 | Method and device for detecting medicinal property of traditional Chinese medicine |
| CN106525821B (en) * | 2016-10-31 | 2019-03-22 | 山东省医药生物技术研究中心 | A kind of method and device detecting herbal nature |
| CN110398485A (en) * | 2018-04-24 | 2019-11-01 | 南京简智仪器设备有限公司 | A kind of detection device and detection method based on difference Raman technology |
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| CN1168973C (en) | 2004-09-29 |
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