CN1804590B - Method for monitoring crop growth regulation and control - Google Patents
Method for monitoring crop growth regulation and control Download PDFInfo
- Publication number
- CN1804590B CN1804590B CN 200510120908 CN200510120908A CN1804590B CN 1804590 B CN1804590 B CN 1804590B CN 200510120908 CN200510120908 CN 200510120908 CN 200510120908 A CN200510120908 A CN 200510120908A CN 1804590 B CN1804590 B CN 1804590B
- Authority
- CN
- China
- Prior art keywords
- plant
- control
- delayed fluorescence
- regulation
- visible light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a method for monitoring the plant grow adjusting and controlling, which mainly tests the plant green tissue chloroplast light-induction delayed fluorescence intensity on adjusting and controlling condition and compares it with the plant light-induction delayed fluorescence intensity to obtain the plant grow adjusting and controlling effect and degree and the adjusting and controlling effect of the adjusting and controlling factors. The apparatus comprises a split sample darkroom, a temperature control component, a visible light excitation component, a weak light probing component, an A/D converter, a data processing component and a computer.
Description
Technical field
The present invention relates to the detection technique of crop growth environment and situation, particularly a kind of monitoring method of crop growth regulation and control.
Background technology
Along with the development of advent of Knowledge and information industry technology, and accompany and the rapid increase of next inevitable agricultural production pressure, the meticulous agricultural practice thought of development cost-saving synergistic has caused that people pay much attention to.For this technological thought is tried out, various detection technique and detection meanss at crop growth environment and situation have obtained combination rapidly and popularization, and especially information and electronic engineering technology have obtained great integrated and comprehensive.Difference correction global satellite positioning system (DGPS), Geographic Information System (GIS) and remote sensing system (RS) in the meticulous agricultural practice ideological system, have mainly been used at present.Wherein the RS technology is the core that this technical system is obtained agricultural land information.Though it also can obtain and analyze the upgrowth situation of crop, it mainly obtains and analyzes is the in esse room and time otherness information of the farm environment factor (as soil texture, landform, plant nutrient, water cut, disease pest and weed etc.) that influences plant growth.And the time that measure to continue is long, and is engraved in change during extraneous ambient condition, and therefore, the data that record are a series of random numbers, can not reflect that tested crop is at that time the regulation and control situation of growing.The monitoring of actual state to the regulation and control of growing of crop self at any time is easy to be interfered.It is just limited bigger in some practical applications like this, and sensitivity is not very high.This system price is relatively more expensive in addition, is unfavorable for popularizing and promoting in agricultural production.
Summary of the invention
The objective of the invention is to shortcoming and defect at the prior art existence, a kind of manual dexterity, quick is provided, can monitor the grow regulating and controlling effect that grows of regulatory factor of various plants, simultaneously the monitoring method of the crop growth regulation and control that also can monitor the regulating and controlling effect of different plants of identical regulation of plant growth and development factor pair and identical plant different growth periods.
Another object of the present invention is to provide a kind of device of realizing the monitoring method of above-mentioned crop growth regulation and control.
Purpose of the present invention is achieved through the following technical solutions: a kind of monitoring method of crop growth regulation and control, it is characterized in that: detect by growing plants chlorenchyma chloroplast delayed fluorescence intensity under the control condition, compare with the delayed fluorescence intensity of adjoining tree then, obtain grow effect that quilt is regulated and control under this control condition and the degree of being regulated and control of this plant, and the regulating effect of the regulation of plant growth and development factor.
The described regulation of plant growth and development factor refers to all plant endogenous materials that can cause that the plant metabolism process changes and allogenic material (for example: plant hormone, plant growth regulator, nutriment, chemical molecular, atmospheric humidity, gas concentration lwevel etc.).
The monitoring method of this crop growth regulation and control more specifically comprises the steps:
(1) will be placed sample darkroom dark adatpation a period of time by the blade of the live body plant that is growing under the control condition, this dark adatpation time promptly is blade surface temperature Time Created, and described blade surface temperature is adjustable.
(2) use visible light as the even irradiation blade of excitation source, induce the plant leaf chloroplast to produce delayed fluorescence.
(3) utilize the weak light detection assembly to receive, and convert thereof into electric signal from the delayed fluorescence signal behind the disconnected light of measurement plant leaf.
(4) electric signal is converted to numerical information and imports computing machine by analog to digital converter.
(5) utilize data processing software that digital signal is carried out data processing, obtain measuring the delayed fluorescence decay in time of plant leaf.
(6) utilize data processing software to carry out data processing, obtain measuring the integrated intensity of the delayed fluorescence of plant leaf in the certain hour scope.
(7) will be compared with the chloroplast delayed fluorescence intensity of the chlorenchyma of adjoining tree of the same race by the chloroplast delayed fluorescence intensity of the chlorenchyma of the plant under the control condition, obtain grow effect that quilt is regulated and control under this control condition and the degree of being regulated and control of this plant, and the regulating effect of the regulation of plant growth and development factor.
The dark adatpation time is 0～3h in the described step (1); The blade surface temperature is adjustable in 0～45 ℃ of scope.
The intensity of illumination of visible light is 0～3000 μ molm in the described step (2)
-2s
-1, wavelength is 390～770nm; Wherein preferable range is 540～700nm, and the visible light of this preferable range can induce the plant leaf chloroplast to produce delayed fluorescence preferably, and obtains easily, implements fairly simple, convenient.
Visible light is 0～60min as the even irradiation blade time of excitation source in the described step (2).
In the described step (2) visible light as the even irradiation blade of excitation source after, close excitation source, time-delay 0.02～40s, the delayed fluorescence of the chloroplast of opening entry visible light-inducing then.
Time range in the described step (6) is 0～30min.
A kind of device of realizing above-mentioned crop growth regulation and control monitoring method comprises scalable sample darkroom, temperature control assembly, excited by visible light assembly, weak light detection assembly, analog to digital converter, data handling component, computing machine; Excited by visible light assembly and weak light detection assembly are connected with scalable sample darkroom, and the weak light detection assembly is connected with data handling component by analog to digital converter, and described data handling component is connected with computing machine.
Described excited by visible light assembly is a LED illumination part; Described weak light detection assembly comprises optical fiber, photomultiplier transit tube module, optical fiber one end stretches into the darkroom, the other end is connected with the photomultiplier transit tube module, described data handling component is a scm managing system, described scm managing system is connected with described photomultiplier transit tube module by analog to digital converter on the one hand, making the photomultiplier transit tube module be delivered to scm managing system after obtaining signal handles, also be connected with the photomultiplier transit tube module on the other hand, with starting or stoping of control photomultiplier transit tube module by control interface (as analog switch, relay).
Action principle of the present invention is: the present inventor finds after deliberation---for the kindred plant blade of identical health status and isometric growth growth cycle, if it is the same by control condition, its chlorenchyma delayed fluorescence intensity has consistance (delayed fluorescence intensity is measured) under uniform temp; The delayed fluorescence intensity of kindred plant blade with identical photosynthetic rate under equal control condition in the error allowed band much at one; Grow with it regulating effect and regulation and control degree of the photosynthetic rate of plant has very direct relation, is the well-characterized person of regulation and control situation of growing.Generally speaking: it is timing that plant is worked as its effect of being regulated and control of growing, the photosynthetic rate of chlorenchyma is just than the photosynthetic rate height of non-regulation and control, the delayed fluorescence intensity of respective green tissue is just big, the delayed fluorescence intensity height of the chlorenchyma that the delayed fluorescence intensity of the chlorenchyma that regulation and control degree in this case, is big is littler than regulation and control degree; And plant grows the effect regulated and control when negative when it, the photosynthetic rate of the chlorenchyma just photosynthetic rate than non-regulation and control is low, the delayed fluorescence intensity of respective green tissue is just little, the delayed fluorescence intensity of the chlorenchyma that the delayed fluorescence intensity of the chlorenchyma that regulation and control degree in this case, is big is littler than regulation and control degree is low.
Delayed fluorescence strength formula equation is
I refers to the area integral under the delayed fluorescence decay kinetic curve.It is decided by the type of plant, the cycle of growing, health status, by regulating effect with by regulation and control degree and the condition of measuring delayed fluorescence intensity: the temperature when the dark adatpation time of blade, excitation wavelength, firing time, time delay, sample measurement.In unified (as: the dark adatpation time of measuring condition, firing time, time delay is all identical, 24 ℃ of temperature) under the situation, select the crop leaf of the same race of isometric growth health growth cycle, measure its delayed fluorescence intensity, it can be truly and sensitive effect that crop regulated and control and the degree of being regulated and control of reflecting.
Through discovering, green plant organizes the area integral intensity level under the chloroplast delayed fluorescence decay kinetic curve to have specificity, measure in view of the above by green plant under the control condition and organize chloroplast delayed fluorescence intensity, can compare accurate and effect that reaction crop growth sensitivity is regulated and control and the degree of being regulated and control, and the regulating effect of the regulation of plant growth and development factor.
The relative prior art of the present invention has following advantage and effect: because photosynthesis is the basis of growth and development of plants, be the most important the most responsive process in the various metabolic processes of plant, the growth and development of plant regulation and control at first can show as the regulation and control of plant leaf blade photosynthesis metabolism process.The regulating and controlling effect of regulation of plant growth and development factor pair crop is not only with the difference of crop growth phase and difference, but also with the difference of the amount of regulation of plant growth and development factor use and difference.Regulating effect and regulation and control degree have very direct relation because the photosynthetic rate of plant is grown with it, be the well-characterized person of regulation and control situation of growing, the effect of the regulation of plant growth and development factor can cause the change of photosynthetic rate, thereby cause the variation of chloroplast delayed fluorescence, even the very trickle effect of the micro-regulation of plant growth and development factor; Next is only physics and chemistry and detects institute's detected regulating effect of energy and regulation and control degree; The adjusting effect that is only at last that people's naked eyes can be felt to obtain or can observable growth and development of plants be regulated and control.So organize the chloroplast delayed fluorescence to monitor the crop growth regulation and control to have sensitive and characteristics efficiently by measuring green plant.Because kindred plant to the sensing capability degree difference of the different regulation of plant growth and development factors, is therefore organized the chloroplast delayed fluorescence by measuring green plant, can monitor the multiple control methods of growing of crop; Can monitor grow grow regulating effect and the regulation and control degree of regulatory factor of various plants; Can detect not the regulating effect of different plants and identical plant different growth periods and regulation and control degree the kindred plant regulatory factor that grows; Can detect not the regulating effect and the regulation and control degree in different identical plant isometric growth cycles of regulation of plant growth and development factor pair.
Description of drawings
Fig. 1 is the structural representation of apparatus of the present invention.
Fig. 2 utilizes the soybean leaves of device realization shown in Figure 1 at variable concentrations sodium bisulfite (NaHSO
3) variation of regulation and control lower blade chloroplast delayed fluorescence intensity.
Fig. 3 utilizes the variation of the maize leaf of device realization shown in Figure 1 in variable concentrations auxin (IAA) regulation and control lower blade chloroplast delayed fluorescence intensity.
Embodiment
The present invention is described in further detail below in conjunction with embodiment and accompanying drawing, but embodiments of the present invention are not limited thereto.
Embodiment 1
Fig. 1 shows the concrete structure of apparatus of the present invention, as seen from Figure 1, this crop growth regulation and control monitoring device comprise that scalable sample darkroom 1, darkroom switch 2, darkroom handle 3, sample rise as high as the banks 4, temperature control assembly 5, filter plate 6, excited by visible light assembly, weak light detection assembly, analog to digital converter 9, data handling component, input keyboard 12, display 13, usb 14, computing machine 15; Excited by visible light assembly and weak light detection assembly are connected with scalable sample darkroom 1, the weak light detection assembly is connected with data handling component by analog to digital converter 9 (selecting A/D converter for use at this), and described data handling component is connected with computing machine 15 by usb 14. and described scalability darkroom 1 is convenient to live body and is stretched into detection; Described temperature control assembly 5 is made of semiconductor cooler and thermistor, and the temperature control scope is 0～45 ℃, and temperature-controlled precision is ± 0.1 ℃; Described excited by visible light assembly is a LED illumination part 7, and intensity of illumination is at 0～3000 μ molm
-2s
-1Adjustable in the scope; Described weak light detection assembly comprises optical fiber 8, photomultiplier transit tube module 11, and optical fiber 8 one ends stretch into darkroom 1, and the other end is connected with photomultiplier transit tube module 11; Described data handling component is a scm managing system 10, described scm managing system 10 is connected with described photomultiplier transit tube module 11 by analog to digital converter 9 on the one hand, making photomultiplier transit tube module 11 be delivered to scm managing system 10 after obtaining signal handles, also be connected with photomultiplier transit tube module 11 on the other hand, with starting or stoping of control photomultiplier transit tube module by control interface (as analog switch, relay).Computing machine 15 is selected the Pentium IV type microcomputer of Intel Company for use at this; Data processing software is selected VB, EXCEL or ORIGIN software for use at this.
During measurement, will spray variable concentrations NaHSO
3Soybean leaves (fruiting period sprays after 12 hours) placed the sample chamber dark adatpation 5 minutes (blade surface adjustment to 24 ℃), (light intensity is selected 350 μ molm for use to excite 0.6s with the LED lamp
-2s
-1), postpone 0.25s, write down the interior delayed fluorescence deamplification of 5s subsequently.With VB software statistics data, in ORIGIN software, handle and obtain delayed fluorescence intensity, as shown in Figure 2.Further data processing is known: 0.5mM NaHSO
3Regulating effect is remarkable positive-effect (*), and the regulation and control degree is 114.2% of contrast; 1mM NaHSO
3Regulating effect is extremely remarkable positive-effect (* *), and the regulation and control degree is 125.9% of contrast; 8mM NaHSO
3Regulating effect is remarkable negative effect (*), and the regulation and control degree is 78.5% of contrast; 16mM NaHSO
3Regulating effect is extremely remarkable negative effect (* *), and the regulation and control degree is 49.1% of contrast.In addition we know NaHSO
3Being the regulating and controlling effect of positive-effect in 0.25～2mM low strength range, is the regulating and controlling effect of negative effect in 4～16mM high concentration range.
In view of the above as can be known: in the soybean fruiting period, foliage spray 1mM NaHSO
3Can significantly promote growing of soybean plant strain, the degree of being regulated and control of growing is not spray NaHSO
3125.9% of plant; The NaHSO of 4～16mM high concentration
3The inhibiting effect of having grown to the soybean plant strain of fruiting period.
The device that present embodiment adopts is with embodiment 1.Said apparatus and method are applied to maize leaf (the seedling phase, regulate and control (root use 3 hours after) with variable concentrations IAA) placed the sample chamber dark adatpation 30 minutes (blade surface adjustment to 24 ℃), (light intensity is selected 1500 μ molm for use to excite 1s with the LED lamp
-2s
-1), postpone 0.25s, record delayed fluorescence deamplification, as shown in Figure 3, further data processing is known: 10
-5μ M IAA regulating effect is remarkable positive-effect (*), and the regulation and control degree is 114.5% of contrast; 10
-4μ M IAA regulating effect is extremely remarkable positive-effect (* *), and the regulation and control degree is 130.1% of contrast; 10
-1Reaching 1 μ M IAA regulating effect is extremely remarkable negative effect (* *), and the regulation and control degree is respectively 56.6% and 46.4% of contrast.In addition we know IAA is 10
-6～10
-3In the μ M low strength range is the regulating and controlling effect of positive-effect, 10
-2In～1 μ M high concentration range is the regulating and controlling effect of negative effect.
In view of the above as can be known: in the corn seedling phase, root uses 10
-4μ M IAA can significantly promote growing of corn seedling plant, and the degree of being regulated and control of growing is not use 130.1% of IAA plant; 10
-2The IAA of～1 μ M high concentration is to the inhibiting effect of having grown of the milpa of seedling phase.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (6)
1. the monitoring method of crop growth regulation and control is characterized in that comprising the steps:
(1) will be placed sample darkroom dark adatpation a period of time by the blade of the live body plant that is growing under the control condition, this dark adatpation time promptly is blade surface temperature Time Created, and described blade surface temperature is adjustable;
(2) use visible light as the even irradiation blade of excitation source, induce the plant leaf chloroplast to produce delayed fluorescence; Described visible light intensity and wavelength are all adjustable;
(3) utilize the weak light detection assembly to receive, and convert thereof into electric signal from the delayed fluorescence signal behind the disconnected light of measurement plant leaf;
(4) electric signal is converted to numerical information and imports computing machine by analog to digital converter;
(5) utilize data processing software that digital signal is carried out data processing, obtain measuring the delayed fluorescence decay in time of plant leaf;
(6) utilize data processing software to carry out data processing, obtain measuring the integrated intensity of the delayed fluorescence of plant leaf in the certain hour scope;
(7) will be compared with the chloroplast delayed fluorescence intensity of the chlorenchyma of adjoining tree of the same race by the chloroplast delayed fluorescence intensity of the chlorenchyma of the plant under the control condition, obtain grow effect that quilt is regulated and control under this control condition and the degree of being regulated and control of this plant, and the regulating effect of the regulation of plant growth and development factor;
The described regulation of plant growth and development factor is meant that all can cause plant endogenous material and allogenic material that the plant metabolism process changes.
2. the monitoring method of crop growth regulation and control according to claim 1 is characterized in that: the dark adatpation time is 5 minutes or 30 minutes in the described step (1); The blade surface temperature is adjustable in 0～45 ℃ of scope.
3. the monitoring method of crop growth regulation and control according to claim 1 is characterized in that: the intensity of illumination of visible light is 350 μ molm in the described step (2)
-2s
-1Or 1500 μ molm
-2s
-1
4. the monitoring method of crop growth regulation and control according to claim 1, it is characterized in that: described wavelength of visible light is 390～770nm.
5. the monitoring method of crop growth regulation and control according to claim 1 is characterized in that: visible light is 0.6s or 1s as the even irradiation blade time of excitation source in the described step (2); In the described step (2) visible light as the even irradiation blade of excitation source after, close excitation source, time-delay 0.25s, the delayed fluorescence of the chloroplast of opening entry visible light-inducing then.
6. the monitoring method of crop growth regulation and control according to claim 1, it is characterized in that: the time range in the described step (6) is 0～30min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510120908 CN1804590B (en) | 2005-12-28 | 2005-12-28 | Method for monitoring crop growth regulation and control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510120908 CN1804590B (en) | 2005-12-28 | 2005-12-28 | Method for monitoring crop growth regulation and control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1804590A CN1804590A (en) | 2006-07-19 |
| CN1804590B true CN1804590B (en) | 2010-05-12 |
Family
ID=36866682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510120908 Expired - Fee Related CN1804590B (en) | 2005-12-28 | 2005-12-28 | Method for monitoring crop growth regulation and control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1804590B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1885013B (en) * | 2006-07-10 | 2010-10-06 | 华南师范大学 | Method for monitoring plant physiological senescence and screening anti- senescent plant |
| CN103091296B (en) * | 2013-01-23 | 2015-06-17 | 浙江大学 | Plant physical condition detection method based on spectral imaging technology, and device for same |
| CN103675247A (en) * | 2013-12-16 | 2014-03-26 | 贵州省亚热带作物研究所 | Whole tissue culture seedling photosynthetic rate rapid determining device and method |
| CN107079556B (en) * | 2014-09-16 | 2019-03-19 | 生物照明有限责任公司 | For controlling the lighting system and associated method of the circadian rhythm of agricultural product |
| CN106482830A (en) * | 2016-09-29 | 2017-03-08 | 清华大学 | A kind of calibration system of the photon counter based on standard light source and method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1387034A (en) * | 2002-06-18 | 2002-12-25 | 华南师范大学 | Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing |
| JP2004301638A (en) * | 2003-03-31 | 2004-10-28 | Shizuoka Prefecture | Device and method for judging keeping period of flowering plant |
-
2005
- 2005-12-28 CN CN 200510120908 patent/CN1804590B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1387034A (en) * | 2002-06-18 | 2002-12-25 | 华南师范大学 | Method and device for quickly measuring photosynthesizing speed of delay light generated by visiblelight inducing |
| JP2004301638A (en) * | 2003-03-31 | 2004-10-28 | Shizuoka Prefecture | Device and method for judging keeping period of flowering plant |
Non-Patent Citations (4)
| Title |
|---|
| 8－22行 |
| Chenglong Wang et al..A novel method for measuring photosynthesis usingdelayedfluorescence of choroplast.Biosnesors and Bioelectronics20.2004,20454-459. * |
| 图1. |
| 第5页第1行－第6页第7行 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1804590A (en) | 2006-07-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4524473B2 (en) | Method and apparatus for measuring water stress on plants | |
| Wang et al. | Effects of drought stress on photosynthesis and chlorophyll fluorescence images of soybean (Glycine max) seedlings | |
| Ehret et al. | Automated monitoring of greenhouse crops | |
| CN106718363B (en) | Irrigation test method and test platform for fine agriculture | |
| Hui et al. | Canopy radiation‐and water‐use efficiencies as affected by elevated [CO2] | |
| CN1804590B (en) | Method for monitoring crop growth regulation and control | |
| CN102150582A (en) | Intelligent cultivation system of sunlight greenhouse | |
| CN1804589B (en) | Method and apparatus for monitoring plant stress physiology and screening high-resistance plant | |
| CN107535213A (en) | A kind of method for quick and precisely identifying tomato seedling heat resistance | |
| CN2864669Y (en) | Plant growth information acquiring device based on near infrared spectra | |
| Simbeye et al. | Plant water stress monitoring and control system | |
| Rahmat et al. | Red onion growth monitoring system in hydroponics environment | |
| Chen et al. | Using the diurnal variation characteristics of effective quantum yield of PSII photochemistry for drought stress detection in maize | |
| Rahmat et al. | Implementation of real-time monitoring on agricultural land of rice plants using smart sensor | |
| McCauley et al. | Reviewing mini-lysimeter controlled irrigation in container crop systems | |
| URAIRI et al. | Optimization of light-dark cycles of Lactuca sativa L. in plant factory | |
| CN104237191A (en) | Method for quickly determining light intensity and temperature adaptation range of plants and device thereof | |
| CN1885013B (en) | Method for monitoring plant physiological senescence and screening anti- senescent plant | |
| Hadi et al. | Aquaponic Plant Control and Monitoring System Using Iot-Based Decision Tree Logic | |
| Nurminen et al. | Green Thumb Engineering: Artificial intelligence for managing IoT enabled houseplants | |
| Alexandrov | The tendency of the photosynthetic activity of the grapevine genotypes of intraspecific and interspecific origin. | |
| CN102141516A (en) | Method for identifying crop heat resistance with using of delay fluorescence emission spectrum | |
| Cheng et al. | A photosynthesis continuous monitoring system for CAM plants | |
| Rosati et al. | Physiological basis for light use efficiency models | |
| Giacomelli | Monitoring plant water requirements within integrated crop production systems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100512 Termination date: 20121228 |