CN1380099A - Human body immunomodulator modulated by micro-alternating biological electric field and its preparation method - Google Patents
Human body immunomodulator modulated by micro-alternating biological electric field and its preparation method Download PDFInfo
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Abstract
The present invention adopts a Micro-Alternating-field Biotechnology, called MAB for short to simulate vital electric wave to make the specific proteins expressed by yeasts respectively possess the functions of activating, regulating, controlling and correcting the immune genes of B, T, K and NK cells which are of low immunological competence. The biological agent made up by using these specific yeasts can be used for regulating and controlling immune gene of body so as to attain the action of restoring immunological functions of body. Said biological agent can be used for preventing and curing various diseases of several tumors, uremia, hepatitis, senile dementia, gastrointestinal diseases, diabetes and nervous disturbance, and has no toxic side effect.
Description
The present invention relates generally to a kind of human immunity conditioning agent that utilizes micro-alternating bio-electric field (Micro-Alternating-field Biotechnology is called for short MAB) technology production and preparation method thereof. Described immunomodulator is used for ill body, the adjusting of weak body's immunity, and the immunologic function of destruction is restored, thus the purpose that reaches disease-resistant, cures the disease.
Biotechnology is the heat subject of world today's scientific and technical research. Make a general survey of the research of world's biotechnology, major subjects is to concentrate on how to prevent and treat human difficult and complicated illness aspect. But the product that can be applied in so far in the human difficult and complicated illness treatment is very rare. Although a gene has been recognized its sequence, the mechanism of the expressed enzyme mechanism of gene, substrate for enzymatic activity, and the activity of substrate for enzymatic activity still is the difficult problem of current biotechnology research, limiting greatly the development of biotechnology.
As mentioned above, why is biotechnology research in this unfavorable situation? it is considered herein that key is the problem of a research method. What conventional biotechnology research adopted is the methods such as biochemistry, biomolecular science, and these methods all are to be come by the chemical method development of routine. Chemical method is to study the variations such as the chemical combination of non-living matter, decomposition, redox basically. Biology then is lived material, and the per minute per second was all ceaselessly changing when these living matters were per, and older generation's demutation of new generation of dying is alternately constant. Only be the conversion of metabolism, material of material in forming the least unit-cell of body orderly carrying out, never can stop. Current biotechnology research all is actually very incomplete to this tangible material of the human body as life. Research of the present invention thinks that a lived organism should be comprised of the two large divisions the most basically, and a part is visible, the tangible human body, i.e. tangible material; Another part be conventional theory think cannot see, impalpable invisible material one " soul ". Here " soul " of indication is not the sort of feudalistic superstition said " ghost ", but is present in " life electric wave " on each organism. On the life entity of a work, this life electric wave is ceaselessly launched, and after this invisible " life electric wave " lost, life had also just stopped, and the human body also just becomes dead material-dead body (human body). All researchs of the world today all are only to study dead material-human body, and never people's research " life electric wave ". The human body is a kind of dead material in a sense, and " life electric wave " is only material alive, and the human body is the host of " life electric wave " just, i.e. emission source. Only have when the human body and " life electric wave " organically combine and just can be referred to as the material-biology of life. In the research of life science, the research of " life electric wave " is more even more important than the research of the human body in sum! Certainly the research of " life electric wave " must just can be a complete biological study in conjunction with the human body.
Be the epoch of chemical technology high development over nearly 200 years, particularly chemical over 70 years after 20th century, biochemistry, bioengineering etc. have had the development of advancing by leaps and bounds, thereby have created ten hundreds of chemical products. Instrument, the clothes of dress, edible food, ornamental cosmetics and the construction material etc. that use from the mankind all are chemical products; Chemicals makes traditional agricultural become chemical farming, no matter is the fertilizer that uses, or diseases prevention, desinsection, weeding all are chemical substances; To use chemical substance to stimulate the crop Fast Growth also be the chemical substance of using in order to obtain high yield. Medically, particularly occupy 100% market in western countries, also captured nearly 82% market in China. The various chemicalses such as a large amount of antibiotic, hormone, interferon are ubiquitous. No matter the world of today is in the family of oneself or each corner, the world of prosperity, is the tinkling of jades chemicals that meets the eye on every side everywhere. Many scientists think in recent years, and chemical industry is the human development that brings height, but has also brought healthy harm to the mankind simultaneously. This is because a large amount of chemicals has caused the pollution of environment, no matter is human edible food, and the water of still drinking all is subject to the pollution of chemicals to some extent; A large amount of chemicalses, particularly antibiotics, interferons, hormone medicine endanger larger; Even never use antibiotic, even the people who never takes medicine also can't escape the chemical substance brought from agricultural product, meat, egg, milk and their goods to the harm of health. The mankind have absorbed a large amount of chemical substances, have caused queer difficult and complicated illness to cure. This is because various chemical substances have caused human immune system's damage, reduction even disappearance. A large amount of various chemical substances are to the pollution of environment in addition, various pathogenic microorganisms have been brought out, from in recent years in the world various reports as can be known, current pathogenic microorganism or all is greatly improved from pathogenecity no matter on kind. Therefore caused human diseases more and more, more and more stranger, more and more refractory is treated. Very general such as various tumours, hepatitis, diabetes, uremia etc., effectively cure way but go back even to this day neither one. Make a general survey of the various research institutions in the world, a large amount of scientists is engaged in the research of medical science, national governments have dropped into the research of countless financial support medical science, yet various chemicalses are introduced not only traditional disease to the market and are not effectively controlled, and have newly produced on the contrary the assorted disease of various strange difficulties and have ceaselessly challenged to medical circle! In succession put into effect such as AIDS, rabid ox disease etc., medical circle is felt simply helpless!
In sum, medical research direction and the method present according to the world develop down, and not only traditional disease can not get effectively preventing and treating, also can bring out on the contrary more, upgrade, more refractory disease. The way that how to find effective, a safe control disease is the too impatient to wait major issue in the world today.
Purpose of the present invention provides the specific radio wave processing of a kind of usefulness yeast cells to have the method for the yeast cells of immunoregulatory activity with preparation for addressing the above problem.
Another object of the present invention provides the yeast cells that obtains with said method.
Further aim of the present invention provides and contains above-mentioned yeast cells as the immunomodulator of active component.
The present invention further purpose provides above-mentioned yeast cells for the preparation of the purposes in the medicine of the medicine of adjusting, rectification or immune cell activated and treatment tumour.
The present invention adopts " the life electric wave " of micro-alternating bio-electric field (MAB) modulation to activate yeast " latent function gene ", makes these yeast become the specific yeast with human activin immune cell function. The domestication of passing through again under " life electric wave " condition of the little alternating electric field of the present invention is cultivated, then make the biologic product of efficient balance the body immunologic function, and these biologic products are applied to human immunity regulate, make the fast quick-recovery of body of immunologic function decline, damage or disappearance, thereby reach the purpose of preventing and treating diseases.
Why the present invention can successfully obtain the immunomodulator biological products for the various diseases control, and key is to adopt and the diverse method of conventional biotechnology research. Method involved in the present invention, research " life electric wave " can live with sensual necessary condition, utilizes simultaneously " life electric wave " to the regulation and control of the composition of host substance. Successfully obtained the pioneering biological immunomodulator in the world by this utilization. Experiment shows, this immunomodulator has safely, has no side effect, effect is remarkable. Owing to suffer from the diseases such as kinds of tumors, renal failure, uremia, hepatitis B, the immunologic function that causes weakens, damages or lacks, and has the function of fast quick-recovery, thereby makes ill body obtain rehabilitation to those. The characteristics of biologic product of the present invention are:
Show that by a large amount of experiments biologic product of the present invention has following characteristic:
1. adopt little alternation electric wave (MAB) method of simulation immunogene specificity " life electric wave ", fully different from the traditional life scientific research;
2. create the life active compound of overall regulating immunity of organism power;
3. this life active compound has the effect of quick adjustment body's immunity;
4. by the quick adjustment of immunologic function, reach suffer from kinds cancer, the usefulness of the fast quick-recovery body health of patient such as uremia, hepatitis, diabetes;
5. biologic product of the present invention is not Chinese medicine, neither Western medicine, and more or not antibiotics, interferons, steroids etc., but a kind of biologic product of safe without toxic side effect;
6. have extremely strong selectivity, must use different biological immunomodulators for immunity degradation, damage or disappearance that different illness cause.
This specification comprises that following accompanying drawing Fig. 1 is the schematic diagram of gene structure. Fig. 2 is that manual simulation's " life electric wave " activates yeast " latent function gene " method schematic diagram. Fig. 3 is the schematic diagram that the specific yeast environmental suitability is cultivated. Fig. 4 is the schematic diagram that specific yeast enlarges culture process after activating. Fig. 5 is the schematic diagram of specific yeast liquid hybrid technique. Fig. 6 is the schematic diagram of the concentration technology of specific yeast liquid. Fig. 7 is finished product cooling packing process schematic representation. Fig. 8 is the another embodiment key diagram of latent function gene activation device (2).
Below in conjunction with accompanying drawing, each feature of the present invention is described in further detail, consult Fig. 1, Fig. 1 is gene structure display. At this, mechanism of the present invention is described first.
Various diseases are formidable enemies of human health, also are to cause human dead main factor. A large amount of studies show that, be which type of disease is all relevant with the immunity of body. Various diseases can not occur when the immunity of body is stronger. But along with the increase at age and wearing out of body, particularly various chemical substances, various antibiotic, hormone etc. cause the damage of immunity of organisms cell, the immunocyte metabolic disorder, immunogene can not be expressed normally, causes the immunologic function decline. The body of immunity decline is easy to be subject to the invasion and attack of various pathogen, also can be subject to the harm of various noxious material, thereby causes various diseases to occur. The decline of immunity of organisms is to be difficult to find according to the detection method of routine. Because detecting body, conventional method can find that B, T, the immunocyte quantity such as K, NK in the body are normal. Research of the present invention thinks whether the quantity of immunocyte can only be one of body's immunity index normally, but does not represent that the immunogene expression is normal, can not indicate that more immunoenzymatic character, activity also are normal. What of B, T, K, NK cell quantity are the immunologic function of immunocyte should not be in other words, will recognize also whether the expressed immunoenzymatic character of immunogene in these immunocytes is normal, and whether immunoenzymatic catalytic activity is enough. It is considered herein that, not only quantity is sufficient for the expressed immuno-enzymatic of immunogene, and enough catalytic effects must be arranged, that is to say, as body B, T, K, when the NK cell quantity is normal, expressed immuno-enzymatic quantity might not capacity, when immuno-enzymatic quantity is enough, also good catalytic activity might not be arranged. Immunogene is the same with other genes, is divided into the two large divisions according to its effect, sees shown in Fig. 1, and the left part of figure is the promoter part of immunogene, comprising promotor gene. Promotor gene is being undertaken the functions such as time that start the immunoenzymatic quantity of right side functional structure gene expression, expression. The right side of figure is called the functional structure Gene Partial, wherein mainly be structural gene, structural gene has determined the structure of the expressed enzyme of this gene, it is enzymatic property, that is to say as long as the coiled structure of structural gene base sequence and gene strand is constant, its expressed enzymatic structure and character just can not change, but its expressed quantity and expression time, when express the enzyme amount few, when express the enzyme amount more, when express enzyme and arrive top etc., be subjected to the control of promotor gene fully. When the promoter of the immunogene of B, T, K, NK immunocyte partly is subject to the impact of various injurious factors, when causing abnormal expression, directly have influence on the expressed immuno-enzymatic quantity of functional structure gene, expression time curve. When being subject to extrinsic factor, the functional structure Gene Partial of immunogene disturbs, when causing its base or curly form to change, can affect the expressed immunoenzymatic character of functional structure gene or activity, the immunity of body has been suffered heavy damage, but conventional detection and be not easy to find. In addition when the expressed immuno-enzymatic quantity of immunogene, when character is constant, immunogene affects injurious factor, whether make time of expression response consistent also is very important, Here it is said immunogene responsibility, responsibility decline when immunogene, or response time in advance or the decline that all can show immunity when lagging behind, and causes body ill. Does why the immunogene of B, the T of body, K, NK cell have the problems referred to above? many endotoxin materials that produce with anion or cationic " free radical ", various pathogenic microorganism are found in research of the present invention, and multiple neurological disorder, various radiation sources etc. all may cause B, T, K, NK immunocyte genetic mutation or " ossifing ", to this rigid gene, be referred to as in the present invention " recessive gene ", this recessive gene can not be in time, express accurately, pathogenic microorganism and multiple virulence factor can not be resisted timely to external world, cause body ill.
Years of researches of the present invention are found, various immunogenes are in vital movement process separately, one species specific " life electric wave " launched in the capital, " life electric wave " difference that different immunogenes is launched, immunogene is made immune response by these " life electric waves " transmission immunologic information to the injurious factor of infringement body. Therefore when immunogene changed, " the life electric wave " launched will change, and that pipe is that a kind of small variation all can cause the variation of " life electric wave ". Key of the present invention has been to find that gene " life electric wave " can cause change because of injurious factor, but also can under with the regulation and control of useful electric wave material, recover normal, caused " recessive gene " of " ossifing " by injurious factor, can use useful factor activator. The frequency range that can be used for electric wave of the present invention (MAB) is 1800MHz-56000MHz, preferred 3500-36000MHz, more preferably 7000-11500MHz. The intensity of electric wave is 115-445mv/cm.
The present invention is according to above principle, adopt the artificial electric wave of simulation body immunogene " life electric wave ", create a kind of " the useful factor ", and this useful factor material made biologic product, regulate and control the immunogene of variation with this biologic product, thereby reach the effect of regulating immunologic function. By the regulation and control immunologic function, make body strengthen the ability of resisting disease, make the fast quick-recovery of body of suffering from multiple sufferer.
The present invention thinks by a large amount of research, obtain a kind of active material that can regulate and control immunogene by manual simulation (MAB) " life electric wave ", is a very difficult job. The present invention is through the exploration discovery in more than 20 years, and the human use's only is " a drop in the ocean " in the ubiquitous microorganism of nature, this be because people to the microbial gene function solve few. Even to this day, the human microorganism of understanding the full gene constitute and function only has 26 kinds, and these 26 kinds of microorganisms also only are kinds simple in structure, does not also understand for the eukaryotic microorganisms mankind of complexity. Particularly in the human yeast microorganism that often uses, contain the gene that is not utilized in a large number, these genes have become " gene ossifys " owing to can not get for a long time using, and the present invention claims that these " gene ossifys " are " latent function gene ". What is more important exists required for the present invention wanting in these " latent function genes ", can be used in the useful protein matter of regulation and control human body immunologic function. The present invention utilize the method for manual simulation's life electric wave activate in the yeast can expression regulation body's immunity protein substance " latent function gene " realize.
The present invention adopts (MAB) simulation life electric wave method to activate yeast " latent function gene ", has cultivated 4 kinds of specific yeasts that activate respectively B, T, K, NK4 kind immunocyte, has made the biologic product of regulation and control body's immunities. The present invention for make this 4 species specific yeast after edible smoothly by stomach, and guarantee can not killed by a large amount of acidic materials under one's belt, the present invention has done this 4 species specificity yeast again the condition of anti-pH≤2.5 and has cultivated. Yeast cells after the cultivation will arrive small intestine by the stomach organ smoothly. The specific yeast cell is cleaved under the effect of small intestine plurality of enzymes, discharges to be packaged in intracellular specific immune function regulatory enzyme (albumen). The expression of immunogene in the activation of these specific immune function regulatory enzymes " selectivity ", the corresponding immunocyte of regulation and control body. 4 kinds of Organism immunoregulation specific yeasts involved in the present invention because they all are the human long-term edible or upper microorganisms of using of production, can not produce any toxic and side effect. The organized enzyme that discharges in these specific yeast cells, have the moment activation, regulate the effect that the interior immunogene of body is correct, efficiently express, then will be absorbed along with the metabolism in the body loses the nutriment that activity is transformed into body very soon, residual in can not causing in body.
The mechanism of action brief description of (MAB) regulation and control body's immunity biologic product involved in the present invention is as follows: the nucleus in the regulation and control body's immunity biologic product is 4 kinds and has the albumen that activates respectively body B, T, K, NK " latent immunity gene " that these 4 kinds of albumen contain respectively again in 4 species specificity yeast. This 4 primary yeast is exactly the microorganism that the present invention adopts manual simulation's " life electric wave " to activate, and claims that in the present invention these the 4 kinds yeast that simulated " life electric wave " activation " latent function gene " are specific yeast. Contain respectively the immunoloregulation function enzyme that is activated in this 4 species specificity yeast cells, these immunoloregulation function enzyme specificity ground activate immune B cell in the body, immune t-cell, immune t-cell and the recovery of immune NK cellular immunity gene, activation, and these immunogenes are correctly efficiently expressed. These specific yeast are cultivated out by separately specific simulation " life electric wave " condition. Activation body B cellular immune function specific yeast used in the present invention, employed (MAB) manual simulation's wave frequency and received-signal strength are to be determined by the immunogene specificity " life electric wave " of immune B cell; Activate body T cellular immune function specific yeast, employed (MAB) manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune t-cell determines; Activate body K cellular immune function specific yeast, employed (MAB) manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune K cell determines; Activate body NK cellular immune function specific yeast, employed (MAB) manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune NK cell determines. The specific yeast of these 4 kinds of difference in functionalitys is cultivated through recessive gene activation, (MAB) simulation " life electric wave " condition, makes and has produced the organized enzyme (albumen) that has activation, regulates and control above-mentioned 4 kinds of immunogenes in the cell. When using this 4 species specificity yeast preparation, specific yeast enters in the small intestine of body, lysis under the effect of small intestine plurality of enzymes, and the cell after the cracking discharges immunogene and activates the regulation and control enzyme. These immunogenes activate regulation and control enzymes and are entered blood by intestinal absorption immediately, are transported to respectively in 4 kinds of immunocytes by blood, thereby reach the purpose of activations, 4 kinds of immunogene corrections raisings of regulation and control immunity.
Immunologic function biological regulator of the present invention is that the step by following two aspects realizes. First step is to adopt specific (MAB) simulation " life electric wave " to activate common yeast, these yeast is become have the specific yeast of regulating B, T, K, NK cellular immune function, and these yeast are made anti-low pH condition cultivate; Second step is under the condition of special (MAB) simulation life electric wave, enlarges and cultivates these specific yeasts, then these specific yeasts is made the biological products that the regulation and control immunologic function is used. The implementation process of these two aspects is to realize by following step. One. activate yeast function " recessive gene "
In above narration, illustrated that the present invention adopts micro-alternating bio-electric field (MAB) simulation " life electric wave " method to activate common yeast, make latent function gene resurrection in the yeast, give expression to respectively the albumen that has activation, regulates and control B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene function. As everyone knows, common yeast is the zymophyte of the many kinds of substances such as class fermentation starch, carbohydrate, protide, multiplex bacterial classification in brewing alcoholic beverages, make bread, make various food, make medicine and multiple product thereof, although saccharomycetic various in style, Various Functions, but never the people utilizes the expressed specific proteins of yeast, activate respectively, regulate B, T, K, NK immunogene function, to carry out recessive gene in the method for not using patent of the present invention be not possess above-mentioned functions before activating to these yeast cells certainly.
(1) activate the method step that is used for regulation and control B cellular immune function gene yeasts " latent function gene ":
A large amount of gene technology studies have shown that a complete B cellular immune function gene forms (seeing Fig. 1) by the two large divisions, and a part is the promotor gene of B cellular immune function gene, and another part is the structural gene of B cellular immune function gene. The structural gene of B cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control B cellular immune function gene of B cellular immune function gene. Therefore, keep the character of the expressed enzyme of B cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of B cellular immune function gene constant; The structural gene that reaches B cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of B cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation B cellular immune function gene protein.
Consult Fig. 2, employing manual simulation involved in the present invention " life electric wave " activates regulation and control B cellular immune function gene yeasts " latent function gene " and adopts this figure or micro-alternating bio-electric field (MAB) device shown in Figure 8, and concrete method step is as follows:
1. according to the one-tenth assignment system culture medium of table 1, and sterilization treatment,
2. select suitable yeast specie (can select yeast to see Table 3), according to active yeast cell/culture medium 〉=1 * 108The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container is T1, cultivated H1 hour,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted to F1,
5. electric wave transmitter output electromagnetic field intensity is adjusted to V1,
6. the blake bottle of Yeast Cultivation liquid will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency will be adjusted on the frequency consistent with transmitter institute tranmitting frequency. The distance of regulating simultaneously between generator and the receiving instrument is L1, and { be 100cm such as distance according to determined distance according to the output received-signal strength that 115-445mv/cm requires to calculate emitter, the output received-signal strength of its emitter is: (115-445mv/cm) * and 100=11.5-44.5v}
7. under these conditions, and keep the T2 temperature conditions, activate H2 hour,
8. then yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation.
(2) activation is similar with regulation and control B cellular immune function gene yeast principle and step for the method step of modulating T cell immunologic function gene yeast " latent function gene ", that is:
Consult Fig. 2,
1. according to the one-tenth assignment system culture medium of table 1, and sterilization treatment,
2. select suitable yeast specie (can select yeast to see Table 3), according to active yeast cell/culture medium 〉=1 * 108The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container is T1, cultivated H1 hour,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted to F2,
5. electric wave transmitter output electromagnetic field intensity is adjusted to V1,
6. the blake bottle of Yeast Cultivation liquid will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency will be adjusted on the frequency consistent with transmitter institute tranmitting frequency. The distance of regulating simultaneously between generator and the receiving instrument is L1, and { be 100cm such as distance according to determined distance according to the output received-signal strength that 115-445mv/cm requires to calculate emitter, the output received-signal strength of its emitter is: (115-445mv/cm) * and 100=11.5-44.5v}
7. under these conditions, and keep the T2 temperature conditions, activate H2 hour,
8. then yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation, and Here it is has the T of expression cellular immune function gene yeast.
(3) (MAB) Tone is controlled B cellular immune function gene yeast principle Yu Bu Sudden Class seemingly, that is: together to activate the method step that is used for regulation and control K cellular immune function gene yeasts " latent function gene "
Consult Fig. 2,
1. according to the one-tenth assignment system culture medium of table 1, and sterilization treatment,
2. select suitable yeast specie (can select yeast to see Table 3), according to active yeast cell/culture medium 〉=1 * 108The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container is T1, cultivated H1 hour,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted to F3,
5. electric wave transmitter output electromagnetic field intensity is adjusted to V1,
6. the blake bottle of Yeast Cultivation liquid will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency will be adjusted on the frequency consistent with transmitter institute tranmitting frequency. The distance of regulating simultaneously between generator and the receiving instrument is L1, and { be 100cm such as distance according to determined distance according to the output received-signal strength that 115-445mv/cm requires to calculate emitter, the output received-signal strength of its emitter is: (115-445mv/cm) * and 100=11.5-44.5v}
7. under these conditions, and keep the T2 temperature conditions, activate H2 hour,
8. then yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make pulvis and preserve, and Here it is desiredly has an expression regulation K cellular immune function gene yeast.
(4) activation is similar with (MAB) regulation and control B cellular immune function gene yeast principle and step for the method step of regulation and control NK cellular immune function gene yeasts " latent function gene ", that is:
Consult Fig. 2,
1. according to the one-tenth assignment system culture medium of table 1, and sterilization treatment,
2. select suitable yeast specie (can select yeast to see Table 3), according to active yeast cell/culture medium 〉=1 * 108The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container is T1, cultivated H1 hour,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted to F4,
5. electric wave transmitter output electromagnetic field intensity is adjusted to V1,
6. the blake bottle of Yeast Cultivation liquid will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency will be adjusted on the frequency consistent with transmitter institute tranmitting frequency. The distance of regulating simultaneously between generator and the receiving instrument is L1, and { be 100cm such as distance according to determined distance according to the output received-signal strength that 115-445mv/cm requires to calculate emitter, the output received-signal strength of its emitter is: (115-445mv/cm) * and 100=11.5-44.5v}
7. under these conditions, and keep the T2 temperature conditions, activate H2 hour,
8. then yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make pulvis and preserve, the specific yeast of Here it is desired expression regulation NK immunologic function gene protein. Two. the domestication of specific yeast acidproof (anti-low pH)
Illustrated that more than the present invention adopts the method for manual simulation's " life electric wave ", activate respectively yeast difference " latent function gene ", obtain 4 kinds of specific yeasts that are respectively applied to activate, regulate and control B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene function. But this 4 species specificity yeast can't be directly used in the various immunogenes of regulation and control, and this is because they can't adapt to the interior environment of body. As everyone knows, be a very complex environment in the body, and various envirment factor is all changing all the time. When this 4 species specific yeast enters the approach of small intestine by oral cavity, stomach, be difficult to ensure the integrality of its cell, more therefore the activity of purpose enzyme in the Customers ' Legal Right yeast cells ensures that the yeast cells activity is very crucial. The present invention has adopted the envirment factor of 4 species specificity yeast (MAB) domestication cultivation, and the little alternating electric field device (MAB) that adopts Fig. 3 is cultivated in domestication, and the concrete grammar step is as follows:
The domestication of the environmental suitability of 4 kinds of functional microorganisms of the present invention is that the method by as shown in Figure 3 realizes:
Consult Fig. 3, the method step of the present invention's 4 species specificity yeast environmental suitabilities domestication is respectively described below: the specific yeast step of (one) domestication regulation and control B cellular immunity gene
1. the method according to table 2 configures culture medium, and sterilization treatment,
2. the culture medium 1000ml that gets in the step 1 is injected in the container of Fig. 3,
3. get specific yeast liquid 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control B cell8It is individual/as ml), to inject container shown in Figure 3,
4. open wave generator as shown in Figure 3, and be adjusted on the specific yeast selectivity frequency F1 of regulation and control B cellular immunity gene,
5. the electric wave output voltage of regulating as shown in Figure 3 is V2=5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 2-90 ℃ temperature conditions is cultivated H3=48-96 hour, separate under the condition that is kept at T3=-2-15 ℃ for subsequent use. (2) the specific yeast step of domestication modulating T cell immunogene
1. the method according to table 2 configures culture medium, and sterilization treatment,
2. the culture medium 1000ml that gets in the step 1 is injected in the container of Fig. 3,
3. get specific yeast liquid 10ml (yeast juice living cells content 〉=1 * 10 of modulating T cell8It is individual/as ml), to inject container shown in Figure 3,
4. open wave generator as shown in Figure 3, and be adjusted on the specific yeast selectivity frequency F2 of modulating T cell immunogene,
5. the electric wave output voltage of regulating as shown in Figure 3 is V2=5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 2-90 ℃ temperature conditions is cultivated H3=48-96 hour, separate under the condition that is kept at T3=-2-15 ℃ for subsequent use. (3) the specific yeast step of domestication regulation and control K cellular immunity gene
1. the method according to table 2 configures culture medium, and sterilization treatment,
2. the culture medium 1000ml that gets in the step 1 is injected in the container of Fig. 3,
3. get specific yeast liquid 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control K cell8It is individual/as ml), to inject container shown in Figure 3,
4. open wave generator as shown in Figure 3, and be adjusted on the specific yeast selectivity frequency F3 of regulation and control K cellular immunity gene,
5. the electric wave output voltage of regulating as shown in Figure 3 is V2=5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 2-90 ℃ temperature conditions is cultivated H3=48-96 hour, separate under the condition that is kept at T3=-2-15 ℃ for subsequent use. (4), the specific yeast step of domestication regulation and control NK cellular immunity gene
1. the method according to table 2 configures culture medium, and sterilization treatment,
2. the culture medium 1000ml that gets in the step 1 is injected in the container of Fig. 3,
3. get specific yeast liquid 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control NK cell8It is individual/as ml), to inject container shown in Figure 3,
4. open wave generator as shown in Figure 3, and be adjusted on specific yeast selectivity (MAB) the frequency F4 of regulation and control NK cellular immunity gene,
5. the electric wave output voltage of regulating as shown in Figure 3 is V2=5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 2-90 ℃ temperature conditions is cultivated H3=48-96 hour, separate under the condition that is kept at T3=-2-15 ℃ for subsequent use. Three. the method for making of immune function controlling agents of the present invention
The method for making of biologic product of the present invention mainly is divided into the cultivation, mixing of specific yeast, the master operation such as concentrated, canned, schematically as follows: the culture process of (one) 4 species specificity yeast
Specific yeast involved in the present invention is cultivated totally 4 kinds. This 4 species specificity yeast only is to have obtained seed after obtaining through said method, make a large amount of biologic products of the present invention, and the specific yeast of capacity must be arranged, and therefore needs to enlarge and cultivates. The present invention sends into the hybrid technique of 4 primary yeast liquid respectively through 4 species specificity yeast juices of above specific yeast culture process acquisition. 4 species specificity Yeast Cultivation are to distinguish by the following method step realization:
The specific yeast culture process of A, adjusting B cellular immunity gene function
The specific yeast culture process engineering of adjusting B cellular immune function involved in the present invention as shown in Figure 4.
Consult specification Fig. 4 of the present invention, the specific yeast culture process step of adjusting B cellular immune function involved in the present invention is as follows:
1. according to the one-tenth assignment system culture medium of table 4, and after sterilization treatment, be injected into respectively in A, B, the C tank of Fig. 4.
2. will activate through manual simulation (MAB) life electric wave method, and the specific yeast of the adjusting B cellular immune function that obtains of the cultivation through tolerating low pH (less than pH2.5), be input among the seeding tank A shown in Fig. 4, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.
3. adjusting wave generator, making the biological electric wave of its output (MAB) is F1, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).
4. keep in above-mentioned (MAB) wave frequency and the constant situation of received-signal strength, cultivated H4=12-96 hour under the T4=2-80 ℃ of condition.
5. when the specific yeast living cells of regulating B cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique.
The specific yeast culture process of B, adjusting T cellular immunity gene function
The specific yeast culture process engineering of adjusting T cellular immune function involved in the present invention as shown in Figure 4.
Consult specification Fig. 4 of the present invention, the specific yeast culture process step of adjusting T cellular immune function involved in the present invention is as follows:
1. according to the one-tenth assignment system culture medium of table 4, and after sterilization treatment, be injected into respectively in A, B, the C tank of Fig. 4.
2. will activate through manual simulation (MAB) life electric wave method, and the specific yeast of the adjusting T cellular immune function that obtains of the cultivation through tolerating low pH (less than pH2.5), be input among the seeding tank A shown in Fig. 4, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.
3. adjusting wave generator, making the biological electric wave of its output (MAB) is F2, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).
4. keep in above-mentioned (MAB) wave frequency and the constant situation of received-signal strength, cultivated H4=12-96 hour under the T4=2-80 ℃ of condition.
5. when the specific yeast living cells of regulating T cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique.
The specific yeast culture process of C, adjusting K cellular immunity gene function
The specific yeast culture process engineering of adjusting K cellular immune function involved in the present invention as shown in Figure 4.
Consult specification Fig. 4 of the present invention, the specific yeast culture process step of adjusting K cellular immune function involved in the present invention is as follows:
1. according to the one-tenth assignment system culture medium of table 4, and after sterilization treatment, be injected into respectively in A, B, the C tank of Fig. 4.
2. will activate through manual simulation (MAB) life electric wave method, and the specific yeast of the adjusting K cellular immune function that obtains of the cultivation through tolerating low pH (less than pH2.5), be input among the seeding tank A shown in Fig. 4, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.
3. adjusting wave generator, making the biological electric wave of its output (MAB) is F3, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).
4. keep in above-mentioned (MAB) wave frequency and the constant situation of received-signal strength, cultivated H4=12-96 hour under the T4=2-80 ℃ of condition.
5. when the specific yeast living cells of regulating K cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique.
The specific yeast culture process of D, adjusting NK cellular immunity gene function
The specific yeast culture process engineering of adjusting NK cellular immune function involved in the present invention as shown in Figure 4.
Consult specification Fig. 4 of the present invention, the specific yeast culture process step of adjusting NK cellular immune function involved in the present invention is as follows:
1. according to the one-tenth assignment system culture medium of table 4, and after sterilization treatment, be injected into respectively in A, B, the C tank of Fig. 4.
2. will activate through manual simulation (MAB) life electric wave method, and the specific yeast of the adjusting NK cellular immune function that obtains of the cultivation through tolerating low pH (less than pH2.5), be input among the seeding tank A shown in Fig. 4, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.
3. adjusting wave generator, making the biological electric wave of its output (MAB) is F4, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).
4. keep in above-mentioned (MAB) wave frequency and the constant situation of received-signal strength, cultivated H4=12-96 hour under the T4=2-80 ℃ of condition.
5. when the specific yeast living cells of regulating NK cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique. The hybrid technique of (two) 4 species specificity yeast
4 species specificity yeast juice hybrid techniques involved in the present invention are to carry out according to the ratio of following table. Specific yeast liquid mixed proportion list (in the 4000L mixed liquor)
The mixing of specific yeast liquid of the present invention is to carry out according to the mode of Fig. 5. Consult Fig. 5, this technique realizes by following steps:
| Specific yeast liquid kind | Quantity | Ratio | Requirement |
| Regulation and control B cellular immunity gene function yeast juice | 1000L | 25％ | In nutrient solution |
| Modulating T cell genetic immunization function yeast juice | 1000L | 25％ | In nutrient solution |
| Regulation and control K cellular immunity gene function yeast juice | 1000L | 25％ | In nutrient solution |
| Regulation and control NK cytogene immunologic function yeast juice | 1000L | 25％ | In nutrient solution |
1. 4 species specificity yeast juices are input to respectively in A, B, C, the D tank.
2. 4 species specificity yeast juices in A, B, C, the D tank are input among the blending tank M according to the ratio of equivalent and mix.
3. mixed yeast juice is injected in the concentration technology shown in Figure 6, prepares concentrated. (3) specific yeast concentration technology with Fig. 6 explanation, is consulted Fig. 6, and concentration technology is with the mixed liquid concentration of 4 species specificity yeast juices in the above-mentioned hybrid technique, and concentrated purpose is that the requirement when reaching the embedding finished product is set. Concentration technology is divided into two-stage, and it is concentrated to be transported to the second level after the first order is concentrated again, and finally reaching enrichment is about 64%.
Concentrating of specific yeast liquid involved in the present invention, realize according to following steps:
1. will through the mixed mixed liquor of above-mentioned hybrid technique, be transported to concentration technology first order thickener equipment shown in Figure 6.
In first order concentration tank with the specific yeast mixed liquor, be concentrated into 80% (measurement basis calculation), then be transported in the thickener of the second level concentrated. Concentrated can adopt that freezing vacuum is concentrated, the normal temperature partial vacuum is concentrated or the method such as concentrated of heating, but which kind of concentrated mode all must be take the activity that keeps the specific yeast cell as benchmark.
3. again be concentrated into 80% in the concentration tank of the second level, relevant concentrated requirement is still with the condition in the step 2. Be transported to immediately dosing technology after concentrated and prepare potting. (4) dosing technology with Fig. 7 explanation, is consulted Fig. 7, and dosing technology involved in the present invention is divided into cooling, metering and three processes of embedding. The purpose of cooling is that the temperature rise that causes in the concentration process will be got off, in order to avoid produce gas after the embedding; Metering is to concentrate the control requirement that whether reaches total amount in order to check, and prepares for the used bottle quantity of embedding; Embedding is the final step of specific yeast liquid being made the finished product main technique. This technique is to realize according to step shown in Figure 7:
1. the specific yeast mixed liquor after will concentrating is transported in the cooling tank shown in Figure 7, is cooled to 15 ℃ of 12-.
2. will through the mixed liquor of cooling tank cooling, be transported in the measuring tank and measure. Mixed liquor after the metering is transported to the embedding of embedding machine, makes finished product.
Below will specifically describe embodiment of the present invention by specific embodiment. Although only relate to a kind of concrete saccharomycete in each embodiment, the inventor finds also can obtain identical result with other saccharomycete bacterial strain by experiment.
The description embodiment 1 of embodiment: activate the method that is used for regulation and control B cellular immune function gene yeasts " latent function gene ":
1. according to the one-tenth assignment system culture medium 1000-2000ml of table 1, and sterilization treatment,
2. select the IFFI1021 yeast specie, according to IFFI1021 cell/culture medium 〉=1 * 10 of living8The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container was cultivated H1=12-96 hour between T1=2-80 ℃,
4. open (MAB) electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted in F1=7200-10700MHz scope,
5. electric wave transmitter output electromagnetic field intensity is adjusted to: V1=115-445mv/cm,
6. the blake bottle of yeast IFFI1021 nutrient solution will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency and the tranmitting frequency of transmitter will be adjusted in the identical F1 scope. Regulate simultaneously the distance L 1=1-10 rice between generator and the receiving instrument,
7. under these conditions, and keep T2=2-80 ℃ temperature conditions, activate H2=12-96 hour,
8. IFFI1021 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. Embodiment 2: activate the method that is used for modulating T cell immunologic function gene yeast " latent function gene ":
1. according to the one-tenth assignment system culture medium 1000-2000ml of table 1, and sterilization treatment,
2. select the IFFI1212 yeast specie, according to IFFI1212 cell/culture medium 〉=1 * 10 of living8The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container was cultivated H1=12-96 hour between T1=2-80 ℃,
4. open (MAB) electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted in F2=7500-10500MHz scope,
5. (MAB) electric wave transmitter output electromagnetic field intensity is adjusted to V1=115-445mv/cm,
6. the blake bottle of yeast IFFI1212 nutrient solution will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency and the tranmitting frequency of transmitter will be adjusted in the identical F2 scope. Regulate simultaneously the distance L 1=1-10 rice between generator and the receiving instrument,
7. under these conditions, and keep T2=2-80 ℃ temperature conditions, activate H2=12-96 hour,
8. IFFI1212 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. Embodiment 3: activate the method that is used for regulation and control K cellular immune function gene yeasts " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium 1000-2000ml of table 1, and sterilization treatment,
2. select the IFFI1301 yeast specie, according to IFFI1301 cell/culture medium 〉=1 * 10 of living8The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container was cultivated H1=12-96 hour at T1=2-80 ℃,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted in the F3=8000-11500MHz scope,
5. (MAB) electric wave transmitter output electromagnetic field intensity is adjusted to V1=115-445mv/cm,
6. the blake bottle of yeast IFFI1301 nutrient solution will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency and the tranmitting frequency of transmitter will be adjusted in the identical F3 scope. Regulate simultaneously the distance L 1=1-10 rice between generator and the receiving instrument,
7. under these conditions, and keep T2=2-80 ℃ temperature conditions, activate H2=12-96 hour,
8. IFFI1301 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. Embodiment 4: activate the method that is used for regulation and control NK cellular immune function gene yeasts " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium 1000-2000ml of table 1, and sterilization treatment,
2. select the IFFI1048 yeast specie, according to IFFI1048 cell/culture medium 〉=1 * 10 of living8The ratio of individual/1000ml is injected the culture medium in the container shown in Figure 2,
3. the temperature in the maintenance container was cultivated H1=12-96 hour between T1=2-80 ℃,
4. open electric wave transmitter shown in Figure 2, will export wave frequency and be adjusted in the F4=7300-10800MHz scope,
5. (MAB) electric wave transmitter output electromagnetic field intensity is adjusted to V1=115-445mv/cm,
6. the blake bottle of yeast IFFI1048 nutrient solution will be housed, be installed to the receiver amplifier output according to pattern shown in Figure 2, receive frequency and the tranmitting frequency of transmitter will be adjusted in the identical F4 scope. The distance of regulating simultaneously between generator and the receiving instrument is L=1-10 rice,
7. under these conditions, and keep T2=2-8 ℃ temperature conditions, activate H2=12-96 hour,
8. IFFI1048 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. Embodiment 5. immunomodulators are to the inhibition of 180 ascites tumors
A. get 60 of wates rats, be divided into totally 3 groups of A, B, C, every group of 20 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt 180 ascites tumors, big white mouse is respectively organized in inoculation respectively.
C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid8Individual/ml), dosage is 0.6ml/kg; B organizes to endoxan, and dosage is 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day.
D. dissect to detect afterwards the size of ascites tumor in seven days, such as following table:
Embodiment 6. immunomodulators are to the inhibition of U-14 solid tumor
| Data | Knurl is heavy | Ratio % | Explanation |
| Group | |||
| The C group | 22g ± 4.2/ | 100% (as 100%) | Mean value |
| The B group | 16g ± 3.7/ | -27.2％ | Mean value |
| The A group | 3g ± 0.3/ | -86.4％ | Mean value |
A. get 90 of wates rats, be divided into totally 3 groups of A, B, C, every group of 30 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt the U-14 solid tumor, big white mouse is respectively organized in inoculation respectively.
C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid8Individual/ml), dosage is 0.6ml/kg; B organizes to endoxan, and dosage is 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day.
D. take the size that dissect to detect afterwards ascites tumor in 14 days, such as following table:
Embodiment seven, and this product is called for short this preparation through the taking of 15 kinds of cancer canceration patients of 197 examples of 4 hospitals such as tumour hospital of medical courses in general institute of BeiJing, China, and situation is as follows: entirely organize the patient male sex's 111 examples (56.34%), women's 86 examples (43.66%). Complete 54.92 years old mean age of group (scope 16-87 year). Accept disease kind such as the following table (5) of all patients for medical treatment: the sick table (5) of planting of experimental observation
| The data group | Knurl is heavy | Ratio % | Explanation |
| The C group | 19g ± 2.2/ | 100% (as 100%) | Mean value |
| The B group | 17g ± 1.6/ | -10.5％ | Mean value |
| The A group | 2g ± 0.2/ | -89.5％ | Mean value |
The sick routine number of the sick kind of routine number of planting
Lung cancer 45 malignant lymphomas 12
The cancer of the esophagus 24 brain tumors 9
Breast cancer 47 soft tissue sarcomas 4
Nasopharyngeal carcinoma 12 neck tumours 18
Cancer of the stomach 3 cutaneum carcinomas 3
Prostate cancer 2 carcinomas of uterine body 4
Colorectal cancer 7 Patients with Urinary System Tumors 3
Other are 4 years old
Amount to 197 methods: oral preparation, every day three times, each 30ml, serveing on 30 days is a course for the treatment of. All patients was all taken more than the course for the treatment of. The patient is radiotherapy 170 examples (86.29%) simultaneously, and Radiotherapy dosimetry is 79 examples (48.2%) more than 60Gy; Above 145 examples (88.4%) of 50Gy; Above 160 examples (97.6%) of 40Gy; Only have 4 examples (2.4%) below the 40Gy; 6 routine dosage are not clear. While chemotherapy 27 examples (14.1%). Chemotherapy regimen is the Combination chemotherapy that DDP, BLM, ADM etc. form for basic medicine. Generally all use 2 of chemotherapy regimens more than the cycle. The curative effect of oral preparation of as a result clinical observation is as follows: red blood cell count(RBC), white blood cell count(WBC), platelet count all compare with the result who reaches after treating before treating. Red blood cell increases more than 100,000/ml, and leucocyte increases more than the 100/ml, and blood platelet increases and is more than 10,000/ml effectively. Be invalid when numerical value descends respectively above-mentioned respective value after the treatment. Be constant in the scope of numerical value change between rise and fall before and after the treatment. The indices of clinical observation, the case change before and after auxiliary Radiotherapy chemotherapy medication sees Table 6. Tumor size CR+PR+SD is that effectively ND is invalid. The KPS value rises, decline 10 is divided into " increase " or " minimizing ", and for stable, body weight increases and minimizing 1Kg is recorded as " increase " or " minimizing " within 10 minutes in fluctuation, and fluctuation is constant within 1Kg. Objective observation index such as appetite, sleep, muscle power, stool and urine etc. are all filled in curative effect by the subordinate list regulation. The change of observation index behind oral preparation of table 6
Observation item example number improves constant decline
Red blood cell 196 103 18 75
Leucocyte 196 102 4 90
Blood platelet 195 81 30 84
Tumor size 164 71 93 0
Body weight 195 76 74 45
KPS 193 43 144 6
Appetite 197 45 128 24
Sleep 197 34 143 20
Muscle power 197 45 120 32
196 8 184 4 preparation oral liquid of stool and urine are biological products, on antitumaous effect, should belong to the steady class medicine of cell, therefore take this preparation as the Radiotherapy chemotherapy adjuvant therapy medicaments, should all classify as effectively effectively reaching " constant " (steadily), it is invalid that " reduction " classified as. Effective and the invalid ratio example table 7 of clinical observation project. The efficient observation item example effective routine number of number of oral preparation clinical observation of table 7 project and efficient (95% credibility interval) invalid routine number and inefficiency (95% credibility interval) red blood cell 196 124,63.3% (56.5%, 70.0%) 72,36.7% (30.0%, 43.5%) leucocyte 196 108,55.1% (48.1%, 62.1%) 88,44.9% (37.9%, 51.9%) blood platelet 195 112,57.4% (50.5%, 64.4%) 83,42.6% (35.6%, 49.5%) tumor size 164 164,100% 0,0% body weight 195 152,78.0% (72.1%, 83.8%) 43,22.0% (16.2%, 27.9%) KPS 193 188,97.4% (95.2%, 99.7%) 5,2.6% (0.3%, 4.0%) appetite 197 173,87.8% (83.2%, 92.4%) 24,12.2% (7.6%, 16.8%) sleep 197 177,89.9% (85.6%, 94.1%) 20,10.2% (5.9%, 14.4%) muscle power 197 165,83.8% (78.7%, 88.9%) 32,16.2% (11.1%, 21.3%) stool and urine 196 192,98.0% (96.0%, 99.9%) 4,197 examples of oral the preparation in 2.0% (0.1%, 4.0%), generation all has no side effect. Embodiment 8, and this preparation carries out in the You An of Pekinese hospital to the control of AIDS, and the AIDS VICTIMS is had good therapeutic action. Being responsible for the doctor is Min Jia and Wu Hao doctor. Embodiment 9, and this preparation is just treated 30 B-type hepatitis patients in the hospital of somewhere. Be all effectively. Consult Fig. 8, Fig. 8 is another embodiment key diagram of latent function gene activation device (8), described device also is called micro-alternating bio-electric field (MAB) device, compare with Fig. 2, this figure is depicted as wired active device, and Fig. 2 is wirelessly-enabled devices, act on identical, shown in Fig. 8, the device of present embodiment mainly includes wave generator (81), amplifier (82), gene activation case (83), wherein, wave generator (81) is connected with amplifier (82) phase telecommunication, normally the wired mode by transmission line connects, be provided with radial shield (831) in the gene activation case (83), amplifier (82) is linked on the radial shield (831) in the gene activation case (83) by output line, the bacterial classification that preparation is activated is placed in the gene activation case (83), open wave generator (81) to desired frequency F, amplifier (82) is that the life electric wave of F is amplified with the frequency of input, export by the power that gives provisioning request, be transferred on the radial shield (831), radial shield (831) namely produces the micro-alternating bio-electric field, by frequency F radiation electric wave, activate the latent function gene of the bacterial classification in gene activation case (83). Frequency generator (81) and amplifier (82) are electronic products commonly used, can directly buy or self manufacture in market, and radial shield (831) is the radio wave transmission antenna, can adopt the structures such as tabular, shaft-like, netted. Table 1. activates regulation and control (B, T, K, NK) cell used yeast " latent function gene "
The medium component table
Table 2 Environmental conditions adaptability medium composition tables (for example to 1000ml)
| Medium component | Quantity |
| Sweet mellow wine | 16g |
| K 2HPO 4 | 0.25g |
| MgSO 4·7H 2O | 0.2g |
| NaCl | 0.22g |
| CaSO 4·H 2O | 0.5g |
| CaCO 3 | 6.0g |
| Urea | 0.2-0.5g |
| Serum | 100--300ml |
| Distilled water | 700--900mL |
| Medium components | Quantity | Explanation |
| Jujube juice | 300ml | Dry jujube / water = 1g/5ml made in proportion The supernatant |
| Baccata juice | 500ml | Baccata dry / water = 1g/5ml proportional system Into the supernatant |
| (NH 4) 2SO 4 | 0.25g | |
| K 2HPO 4 | 0.2g | |
| MgSO 4·7H 2O | 0.22g | |
| NaCl | 0.5g | |
| CaSO 4·2H 2O | 0.3g | |
| CaCO 3 | 3.0g | |
| The specificity of activation Yeast culture fluid | The 20ml | Containing live yeast cells ≥ 1 × 108Cells / ml |
Table 3 of this patent microbial species involved
(But not limited to microorganisms listed in this table)
Table 4. Specific yeast expanding medium composition tables (1000L medium to count)
Note: 1. Above table were based on a variety of liquids: Material / water = 1/10 ratio of processed steel.
| View genus -01 | Saccharomyces cerevisiae Hansen Saccharomyces cerevisiae | |||
| Original purpose -01 | Wine, food and food manufacturing | |||
| ACCC2034 | ACCC2035 | ACCC2036 | ACCC2037 | ACCC2038 |
| ACCC2039 | ACCC2040 | ACCC2041 | ACCC2042 | AS2.1 |
| AS2.4 | AS2.11 | AS2.14 | AS2.16 | AS2.56 |
| AS2.69 | AS2.70 | AS2.93 | AS2.98 | AS2.101 |
| AS2.109 | AS2.110 | AS2.112 | AS2.139 | AS2.173 |
| AS2.174 | AS2.182 | AS2.196 | AS2.242 | AS2.336 |
| AS2.346 | AS2.369 | AS2.374 | AS2.375 | AS2.379 |
| AS2.380 | AS2.382 | AS2.390 | AS2.393 | AS2.395 |
| AS2.396 | AS2.397 | AS2.398 | AS2.399 | AS2.400 |
| AS2.406 | AS2.408 | AS2.409 | AS2.413 | AS2.414 |
| AS2.415 | AS2.416 | AS2.422 | AS2.423 | AS2.430 |
| AS2.431 | AS2.432 | AS2.451 | AS2.452 | AS2.453 |
| AS2.458 | AS2.460 | AS2.463 | AS2.467 | AS2.486 |
| AS2.501 | AS2.502 | AS2.503 | AS2.504 | AS2.516 |
| AS2.535 | AS2.536 | AS2.558 | AS2.560 | AS2.561 |
| AS2.562 | AS2.576 | AS2.593 | AS2.594 | AS2.614 |
| AS2.620 | AS2.628 | AS2.631 | AS2.666 | AS2.982 |
| AS2.1190 | AS2.1364 | AS2.1396 | IFFI1001 | IFFI1002 |
| IFFI1005 | IFFI1006 | IFFI1008 | IFFI1009 | IFFI1010 |
| IFFI1012 | IFFI1021 | IFFI1027 | IFFI1037 | IFFI1042 |
| IFFI1043 | IFFI1045 | IFFI1048 | IFFI1049 | IFFI1050 |
| IFFI1052 | IFFI1059 | IFFI1060 | IFFI1063 | IFFI1202 |
| IFFI1203 | IFFI1206 | IFFI1209 | IFFI1210 | IFFI1211 |
| IFFI1212 | IFFI1213 | IFFI1214 | IFFI1215 | IFFI1220 |
| IFFI1221 | IFFI1224 | IFFI1247 | IFFI1248 | IFFI1251 |
| IFFI1270 | IFFI1277 | IFFI1287 | IFFI1289 | IFFI1290 |
| IFFI1291 | IFFI1292 | IFFI1293 | IFFI1297 | IFFI12300 |
| IFFI1301 | IFFI1302 | IFFI1307 | IFFI1308 | IFFI1309 |
| IFFI1310 | IFFI1311 | IFFI1335 | IFFI1336 | IFFI1337 |
| IFFI1338 | IFFI1339 | IFFI1340 | IFFI1345 | IFFI1348 |
| IFFI1396 | IFFI1397 | IFFI1399 | IFFI1411 | IFFI1413 |
| View genus -02 | Saccharomyces cerevisiae Hansen Var.ellipsoideus (Hansen) Dekker oval Saccharomyces cerevisiae | |||
| Original purpose -02 | Wine, food and food manufacturing | |||
| ACCC2043 | AS2.2 | AS2.3 | AS2.8 | AS2.53 |
| AS2.163 | AS2.168 | AS2.483 | AS2.541 | AS2.559 |
| AS2.606 | AS2.607 | AS2.611 | AS2.612 | |
| View genus -03 | Yeast Saccharomyces chevalieri Guilliermond Xue watts | |||
| Original purpose -03 | Wine, food and food manufacturing | |||
| AS2.131 | AS2.213 | |||
| View genus -04 | Saccharomyces delbrueckii Deer Bu yeast | |||
| Original purpose -04 | Food and Fermentation | |||
| AS2.285 | ||||
| View genus -05 | Saccharomyces delbrueckii Lindner ver.mongolicus (Saito) Lodder et van Rij 蒙古德尔布 yeast | |||
| Original purpose -05 | Food and Fermentation | |||
| AS2.209 | AS2.1157 | |||
| -06 Genus belongs | Saccharomyces exiguous Hansen Less yeast spores | |||
| Original purpose -06 | Food and Fermentation | |||
| AS2.349 | AS2.1158 | |||
| View genus -07 | Saccharomyces fermentati (Saito) Lodder et van Rij Yeast fermentation | |||
| Original purpose -07 | Food and Fermentation | |||
| AS2.286 | AS2.343 | |||
| View genus -08 | Saccharomyces Logos van laer et Denamur ex Jorgensen Logue yeast | |||
| Original purpose -08 | Winemaking, food fermentation, etc. | |||
| AS2.156 | AS2.327 | AS2.335 | ||
| View genus -08 | Saccharomyces mellis (Fabian et Quinet) Lodder et kreger van Rij honey yeast | |||
| Original purpose -08 | With sugars, starches fermentation | |||
| AS2.195 | ||||
| View genus -09 | Saccharomyces mellis Microellipsoides Osterwalder Small oval yeast | |||
| Original purpose -09 | With sugars, starches fermentation | |||
| AS2.699 | ||||
| -10 Genus belongs | Saccharomyces oviformis Osteralder Ovoid yeast | |||
| Original purpose -10 | With sugars, starches fermentation | |||
| AS2.100 | ||||
| View genus -11 | Saccharomyces rosei (Guilliermond) Lodder et Kreger van Rij Ross yeast | |||
| Original purpose -11 | With sugars, starches fermentation | |||
| AS2.287 | ||||
| -12 Genus belongs | Saccharomyces rouxii Boutroux Lu's yeast | |||
| Original purpose -12 | With sugars, starch fermentation, manufacture of edible sauce, soy sauce, etc. | |||
| AS2.178 | AS2.180 | AS2.370 | AS2.371 | |
| -13 Genus belongs | Saccharomyces Sake Yabe Sake yeast | |||
| Original purpose -13 | With sugars, starches fermentation | |||
| ACCC2045 | ||||
| View genus -14 | Candida arborea Candida tree | |||
| Original purpose -14 | For feed, aminoacids manufacture, cellulose, starch, sugars, protein Fermentation. | |||
| AS2.566 | ||||
| -15 Genus belongs | Candida lambica (Lindner et Genoud) van.Uden et Buckley; Lang Bike Candida | |||
| Original purpose -15 | Esters for high temperature production, manufacturing food flavor | |||
| AS2.1182 | ||||
| -16 Genus belongs | Candida Krusei (Castellani) Berkhout Cruise yeast | |||
| Original purpose -16 | For wine, manufacture of feed, amino acids, proteins and so on. | |||
| AS2.1045 | ||||
| -17 Genus belongs | Candida lipolytica (Harrison) Diddens et Lodder Candida lipolytica | |||
| Original purpose -17 | For the oil dewaxing, manufacture organic acids | |||
| AS2.1207 | AS2.1216 | AS2.1220 | AS2.1379 | AS2.1398 |
| AS2.1399 | AS2.1400 | |||
| -17 Genus belongs | Candida parapsilosis (Ashford) Langeron et Talice Var. intermedia Van Rij et Verona Medium Smooth Candida | |||
| Original purpose -17 | The use of sugars, starches fermented feed manufacturing | |||
| AS2.491 | ||||
| -18 Genus belongs | Candida parapsilosis (Ashford) Langeron et Talice Candida parapsilosis | |||
| Original purpose -18 | Making use of amyl sugar hydrolyzate feed | |||
| AS2.590 | ||||
| -19 Genus belongs | Candida pulcherriman (Lindner) Windisch Iron red yeast | |||
| Original purpose -19 | Stimulate growth | |||
| AS2.492 | ||||
| -20 Genus belongs | Candida rugosa (Anderson) Diddens et Lodder Candida folds | |||
| Original purpose -20 | Oil dewaxing, the production of organic acids, etc. | |||
| AS2.511 | AS2.1367 | AS2.1369 | AS2.1372 | AS2.1373 |
| AS2.1377 | AS2.1378 | AS2.1384 | ||
| -21 Genus belongs | Candida tropicalis (Castellani) Berkhout Candida tropicalis | |||
| Original purpose -21 | Carbohydrate fermentation; cellulose, hemicellulose fermentation; Pulp fermentation; thionyl Tobacco acid fermentation; feed manufacturers; yeast extract, ergosterol manufacturing. | |||
| ACCC2004 | ACCC2005 | ACCC2006 | AS2.164 | AS2.402 |
| AS2.564 | AS2.565 | AS2.567 | AS2.568 | AS2.617 |
| AS2.637 | AS2.1387 | AS2.1397 | ||
| -22 Genus belongs | Candida utilis Henneberg Lodder et Kreger Van Rij Utilis yeast | |||
| -22 Original purpose | Food and feed manufacturing | |||
| AS2.120 | AS2.281 | AS2.1180 | ||
| -23 Genus belongs | Crebrothecium ashbyii (Guilliermond) Routein = Eremothecium ashbyii Guilliermond Ashdod false capsule yeast | |||
| -23 Original purpose | Manufacturing for riboflavin | |||
| AS2.481 | AS2.482 | AS2.1197 | ||
| -24 Genus belongs | Geotrichum candidum Link Geotrichum | |||
| -24 Original purpose | Feed manufacturers; | |||
| ACCC2016 | AS2.361 | AS2.498 | AS2.616 | AS2.1035 |
| AS2.1062 | AS2.1080 | AS2.1132 | AS2.1175 | AS2.1183 |
| -25 Genus belongs | Hansenula anomala (Hansen) H et P sydow Hansenula anomala | |||
| Original purpose -25 | Perfumery; improve wine flavor, food flavor, etc.; | |||
| ACCC2018 | AS2.294 | AS2.295 | AS2.296 | AS2.297 |
| AS2.298 | AS2.299 | AS2.300 | AS2.302 | AS2.338 |
| AS2.339 | AS2.340 | AS2.341 | AS2.470 | AS2.592 |
| AS2.641 | AS2.642 | AS2.782 | AS2.635 | AS2.794 |
| -26 Genus belongs | Hansenula arabitolenes Fang Arabinitol Hansenula | |||
| -26 Original purpose | Production of arabitol and glycerol; | |||
| AS2.887 | ||||
| -27 Genus belongs | Hansenula jadinii (A.et R Sartory Weill et Meyer) Wickerham Jieding Hansenula | |||
| -27 Original purpose | Application not found reports | |||
| ACCC2019 | ||||
| -28 Genus belongs | Hansenula saturnus (Klocker) H et P sydow Saturn Hansenula | |||
| Original purpose -28 | Application not found reports | |||
| ACCC2020 | ||||
| -29 Genus belongs | Hansenula schneggii (Weber) Dekker Amur Hansenula | |||
| Original purpose -29 | Application not found reports | |||
| AS2.304 | ||||
| -30 Genus belongs | Hansenula subpelliculosa Bedford Asian film Hansenula | |||
| Original purpose -30 | Liquor from a variety of raw materials or bad residue obtained, but not found in the application report | |||
| AS2.740 | AS2.760 | AS2.761 | AS2.770 | AS2.783 |
| AS2.790 | AS2.798 | AS2.866 | ||
| -31 Genus belongs | Kloeckera apiculata (Reess emend.Klocker) Janke Lemon-shaped Klerk yeast | |||
| -31 Original purpose | Application not found reports | |||
| ACCC2022 | ACCC2023 | AS2.197 | AS2.496 | AS2.714 |
| ACCC2021 | AS2.711 | |||
| -32 Genus belongs | Lipomycess starkeyi Lodder et van Rij Grease yeast | |||
| Original purpose -32 | Application not found reports | |||
| AS2.1390 | ACCC2024 | |||
| -33 Genus belongs | Pichia farinose (Lindner) Hansen Powdered Pichia | |||
| -33 Original purpose | Application not found reports | |||
| ACCC2025 | ACCC2026 | AS2.86 | AS2.87 | AS2.705 |
| AS2.803 | ||||
| -34 Genus belongs | Pichia membranaefaciens Hansen Film Bu Pichia | |||
| -34 Original purpose | Application not found reports | |||
| ACCC2027 | AS2.89 | AS2.661 | AS2.1039 | |
| -35 Genus belongs | Rhodosporidium toruloides Banno Rhodosporidium yeast | |||
| Original purpose -35 | Application not found reports | |||
| ACCC2028 | ||||
| -35 Genus belongs | Rhodotorula glutinis (Fresenius) Harrison Red yeast | |||
| Original purpose -35 | Carbohydrate fermentation, fermented protein, manufacture of food, spices, etc. | |||
| AS2.2029 | AS2.280 | ACCC2030 | AS2.102 | AS2.107 |
| AS2.278 | AS2.499 | AS2.694 | AS2.703 | AS2.704 |
| AS2.1146 | ||||
| -36 Genus belongs | Rhodotorula minuta (Saito) Harrison Red yeast | |||
| Original purpose -36 | Carbohydrate fermentation, fermented protein, manufacture of food, spices, etc. | |||
| AS2.277 | ||||
| -37 Genus belongs | Rhodotorula rubar (Demme) Lodder Crimson yeast | |||
| -37 Original purpose | Carbohydrate fermentation, fermented proteins, making food, spices, feed, etc. | |||
| AS2.21 | AS2.22 | AS2.103 | AS2.105 | AS2.108 |
| AS2.140 | AS2.166 | AS2.167 | AS2.272 | AS2.279 |
| AS2.282 | ACCC2031 | |||
| -38 Genus belongs | Saccharomyces carlsbergensis Hansen Kars yeast | |||
| -38 Original purpose | Food manufacturing, brewing, feed, etc. | |||
| AS2.113 | ACCC2032 | ACCC2033 | AS2.312 | AS2.116 |
| AS2.118 | AS2.121 | AS2.132 | AS2.162 | AS2.189 |
| AS2.200 | AS2.216 | AS2.265 | AS2.377 | AS2.417 |
| AS2.420 | AS2.440 | AS2.441 | AS2.443 | AS2.444 |
| AS2.459 | AS2.595 | AS2.605 | AS2.638 | AS2.742 |
| AS2.745 | AS2.748 | AS2.1042 | ||
| -39 Genus belongs | Saccharomyces uvarum Beijer Uvarum | |||
| -39 Original purpose | Food manufacturing, brewing, feed, etc. | |||
| IFFI1023 | IFFI1032 | IFFI1036 | IFFI1044 | IFFI1072 |
| IFFI1205 | IFFI1207 | |||
| -40 Genus belongs | Saccharomyces willianus Saccardo Will yeast | |||
| Original purpose -40 | Food manufacturing, brewing, feed, etc. | |||
| AS2.5 | AS2.7 | AS2.119 | AS2.152 | AS2.293 |
| AS2.381 | AS2.392 | AS2.434 | AS2.614 | AS2.1189 |
| -41 Genus belongs | Saccharomyces sp. Yeast | |||
| Original purpose -41 | Manufacture of brandy, etc. | |||
| AS2.311 | ||||
| -42 Genus belongs | Saccharomycodes ludwigii Hansen Luther Yeast | |||
| Original purpose -42 | Seen reports of applications | |||
| ACCC2044 | AS2.243 | AS2.508 | ||
| -43 Genus belongs | Saccharomycodes sinenses Yue China Yeast | |||
| -43 Original purpose | Seen reports of applications | |||
| AS2.1395 | ||||
| -44 Genus belongs | Schizosaccharomyces octosporus Beijerinck Eight fission yeast spores | |||
| -44 Original purpose | Seen reports of applications | |||
| ACCC2046 | AS2.1148 | |||
| -45 Genus belongs | Schizosaccharomyces pombe Lindner S. pombe | |||
| Original purpose -45 | Lactose fermentation, brewing, feed, etc. | |||
| ACCC2047 | ACCC2048 | AS2.214 | AS2.248 | AS2.249 |
| AS2.255 | AS2.257 | AS2.259 | AS2.260 | AS2.274 |
| AS2.994 | AS2.1043 | AS2.1149 | AS2.1178 | IFFI1056 |
| -46 Genus belongs | Sporobolomyces roseus Kluyver et van Niel Throw yeast spores | |||
| -46 Original purpose | Lactose fermentation, brewing, feed, antibiotics and other | |||
| ACCC2049 | ACCC2050 | AS2.19 | AS2.962 | AS2.1036 |
| ACCC2051 | AS2.261 | AS2.262 | ||
| -47 Genus belongs | Torulopsis Candida (Saito) Lodder White Torulopsis | |||
| -47 Original purpose | ||||
| AS2.270 | ACCC2052 | |||
| -48 Genus belongs | Torulopsis famta (Harrisn) Lodder et van Rij Unnamed Torulopsis | |||
| -48 Original purpose | ||||
| ACCC2053 | AS2.685 | |||
| -49 Genus belongs | Torulopsis globosa (Olson et Hammer) Lodder et van Rij Torulopsis | |||
| -49 Original purpose | ||||
| ACCC2054 | AS2.202 | |||
| -50 Genus belongs | Torulopsis inconspicua Lodder et Kreger van Rij Usually Torulopsis | |||
| Original purpose -50 | ||||
| AS2.75 | ||||
| -51 Genus belongs | Trichosporon behrendii Lodder et Kreger van Rij Bei Leisi yeast spores | |||
| -51 Original purpose | ||||
| ACCC2056 | AS2.1193 | |||
| -52 Genus belongs | Trichosporon capitatum Diddens et Lodder Head-like yeast Trichosporon | |||
| -52 Original purpose | ||||
| ACCC2056 | AS2.1385 | |||
| -53 Genus belongs | Trichosporon cutaneum (de Beurm et al.) Ota Leather-like yeast Trichosporon | |||
| -53 Original purpose | ||||
| ACCC2057 | AS2.25 | AS2.570 | AS2.571 | AS2.1374 |
| -54 Genus belongs | Wickerhamia fluorescens (Soneda) Soneda Wick yeast | |||
| Original purpose -54 | ||||
| ACCC2058 | AS2.1388 | |||
| Medium components | Quantity |
| Hawthorn liquid | 200L |
| Schisandra liquid | 200L |
| Jujube liquid | 200L |
| Big Bean | 200L |
| Apple's solution | 200L |
(2) the culture medium to adjust the table to pH2.5 ± 0.2 range.
Claims (10)
- A method for the yeast into a specific immune function of immune regulation yeast Agent, characterized in that, the following steps, the first step of the alternating micro-organisms Field (MAB), the use of radio waves for each characteristic of yeast, activating its latent function Gene, the second step will activate the function of genes in yeast recessive environmental factors (MAB) Acclimation, the third step will go through domesticated yeast by environmental factors (MAB) to expand Cultured mixture was concentrated at a predetermined type of modulation, that is made of the required human yeast free Immunomodulatory agent.
- (2) as claimed in claim 1, wherein the yeast for producing human immune modulators method of Characterized in that the micro-organisms alternating electric field (MAB) of yeast used in each of the characteristics of wireless Radio activate its recessive genes steps include:(1) preparation of media 500-5000ml, and the sterilization process,(2) Select the type of yeast, in accordance with viable cells / culture ≥ 1 × 108A / 1000ml than Example, into the culture medium within the container,(3) maintaining the temperature in the range of T1, H1 hours cultivation,(4) the use of Figure 2 or Figure 8 of the micro organisms alternating electric apparatus, radio transmitting device to open, The output frequency is adjusted to the respective F-wave range,(5) The electromagnetic wave emitting device output intensity adjustment to: V1 (with a distance of 100cm For example) range,(6) contains the yeast culture solution into the receiver flask amplifier output, Transmitting the reception frequency and the transmit frequency meter adjusted to the same F range, Meter and occurs while adjusting the distance between the receivers L1,(7) Under the above conditions, and to maintain a temperature of T2, activate H2 hours.(8) activated by the above conditions are yeast cells, vacuum freeze-drying method as Ampoules or made into powder saved.
- 3 as claimed in claim 1, wherein the yeast for producing human immune modulators method of Characterized by recessive genes will activate the yeast environmental factors (MAB) domestication Cultured steps include:(1) Configure the medium and sterilized(2) take 1 1000ml injected into the medium in the container three drawings,(3) to take control of a yeast cell a specific liquid 10ml (yeast mixture containing living cells Volume ≥ LT), into the container Figure 3 shows,(4) open as shown in Figure three (MAB) radio wave generator, and adjusted to regulate the kind of Gene-specific cellular immunity specific frequency F on the yeast,(5) Figure 3 shows the wave regulator output voltage V2 (100ml medium that makes The signal strength of a 5-10v),(6) to maintain the (MAB) wave frequency and wave intensity constant, at a temperature of 2-90 ℃ H3 hours culture conditions, the separation of conditions stored in the backup T3.
- 4 as claimed in claim 1, wherein the yeast for producing human immune modulators method of Characterized in that will go through environmental factors (MAB) Enlarged domesticated step of culturing yeast, Comprising the steps of:(1) preparation of the medium, and after sterilization treatment, were injected into the culture tank,(2) will be through artificial (MAB) life wave method to activate, and by low tolerance to pH (small At pH2.5) obtained by adjusting the cultivation of the yeast-specific cellular immune function Mother, entered into the seed tank, as the seed liquid, and then follow a certain proportion Was injected into the seed culture medium to expand,(3) regulation (MAB) radio wave generator to output bio-wave is F, the signal strength As 115-445mv/cm,(4) holding the (MAB) radio frequency and signal strength constant, the T4 Article Piece H4 hour incubation,(5) develop specific live yeast cells reached 2000000000 / ml; And, selecting the desired type of yeast, and various yeast mix Case, proceed as follows mixing, concentration, finished goods, packaging:(1) the specific yeast solutions were input to each tank,(2) to each tank in accordance with the species-specific yeast was mixed with the same amount of proportional input to Mixing together in a tank,(3) the mixed solution was concentrated yeast,(4) the specificity of the concentrated mixture was transferred to the cooling tank yeast, cooled to 2-20℃，(5) The mixture was cooled through the cooling tank, transported to the metering tank measurement, the meter After the mixture was transferred to the amount of potting potting machine to produce the finished product.
- 5 as claimed in claim 1 or 2, wherein a yeast for producing human immune modulators method Wherein, using four different (MAB) characteristic radio frequency F1, F2, F3, F4, respectively, can be formed activated B, T, K, NK cells recessive four kinds of special immune genes Heterosexual yeast.
- As claimed in claim 1 or 2, wherein a yeast for producing human immune modulators method Wherein, when the characteristic frequency range 7200-10700MHz F1 is activated when used Regulation of B cell immune function due to yeast.
- As claimed in claim 1 or 2, wherein a yeast for producing human immune modulators method Wherein use (MAB) characteristic of radio waves recessive genes active yeast The container holding temperature T1 range is 2-80 ℃.
- As claimed in claim 1, wherein the yeast for producing human immune modulators method of Characterized in that the method is applicable to all the yeasts.
- 9 A yeast immune modulators, characterized in that it is produced by the method described in 1 out.
- A process as claimed in claim 9, wherein the yeast immune modulators, characterized in that, It contains the following specific yeast in any one, any two, any three types Any of the four: regulation of B cell-specific immune genes of yeast, the regulation of T cell immunity Specificity of yeast genes, regulating specific genes K yeast cell-mediated immunity, regulate NK cell immune genes specific yeasts.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01110634 CN1380099A (en) | 2001-04-13 | 2001-04-13 | Human body immunomodulator modulated by micro-alternating biological electric field and its preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01110634 CN1380099A (en) | 2001-04-13 | 2001-04-13 | Human body immunomodulator modulated by micro-alternating biological electric field and its preparation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068470A (en) * | 2010-12-30 | 2011-05-25 | 香港生命信息康复院有限公司 | Anticancer oral liquid preparation and main component preparation method thereof |
| CN102085256A (en) * | 2010-12-30 | 2011-06-08 | 香港生命信息康复院有限公司 | Immune regulation oral liquid preparation with anti-cancer efficacy and preparation method of a main component thereof |
| CN101548987B (en) * | 2009-04-10 | 2012-08-22 | 清华大学 | Cell culturing extract for degrading amyloid beta, preparation method and application thereof |
-
2001
- 2001-04-13 CN CN 01110634 patent/CN1380099A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101548987B (en) * | 2009-04-10 | 2012-08-22 | 清华大学 | Cell culturing extract for degrading amyloid beta, preparation method and application thereof |
| CN102068470A (en) * | 2010-12-30 | 2011-05-25 | 香港生命信息康复院有限公司 | Anticancer oral liquid preparation and main component preparation method thereof |
| CN102085256A (en) * | 2010-12-30 | 2011-06-08 | 香港生命信息康复院有限公司 | Immune regulation oral liquid preparation with anti-cancer efficacy and preparation method of a main component thereof |
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