CN1519031A - Biological preparation modified in plasma environment for treating diabetes and preparation method - Google Patents
Biological preparation modified in plasma environment for treating diabetes and preparation method Download PDFInfo
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Abstract
A biomedicine modified by plasma for preventing and treating diabetes is disclosed. The plasma environment is used to generate the simulative vital electric waves for activating the recessive genes of ordinary yeast, so becoming specific yeast whose expressed characteristic protein can activate, regulate and correct the immunity of B, T, K and NK cells. Its advantages are high curative effect and no toxic by-effect.
Description
The present invention relates generally to method and corresponding medicine, particularly biological preparation medicine and the manufacture method thereof of utilizing the plasma environment technology to make medicine.This biological preparation medicine is used for prevention and cures diabetes.
Biotechnology is the heat subject of world today's scientific and technical research.But the biological medicine that can be applied in so far in the human difficult and complicated illness treatment is very rare.Though a gene has been recognized its sequence, the mechanism that gene is expressed, the mechanism of catalytic substrate, and the activity of substrate for enzymatic activity still is the difficult problem of current biotechnology research, limiting development of biology greatly.
As mentioned above, why is biotechnology research in this unfavorable situation? it is considered herein that key is the problem of a research method.What conventional biotechnology research adopted is methods such as biochemistry, biomolecular science, and these methods all are to be come by the chemical method development of routine.Chemical method is to study variations such as the chemical combination of non-living matter, decomposition, oxidoreduction basically.Biology then is lived material, and the per minute per second was all ceaselessly changing when these living matters were per, older generation's demutation of new generation of dying, alternate.The conversion of the metabolism of material, material orderly carrying out in forming least unit-----cell of body never can stop.Current biotechnology research is whole as life this tangible material of the human body, is actually very incomplete.Research of the present invention thinks, lived biology the most basic should be made up of the two large divisions, a part is visible, the tangible human body, promptly tangible material; Another part be conventional theory think cannot see, impalpable invisible material----" soul ".Here " soul " of indication is not the sort of feudalistic superstition said " ghost ", but is present in " life electric wave " on each organism.On the life entity of a work, the emission that this life electric wave does not stop, after this invisible " life electric wave " lost, life had also just stopped, and the human body also just becomes dead material-----dead body (human body).All researchs of the world today all are only to study dead the material----human body, and never people's research " life electric wave ".The human body is a kind of dead material in a sense, and " life electric wave " is only material alive, and the human body is the host of " life electric wave " just, i.e. emission source.Have only when the human body and " life electric wave " organically combine and just can be referred to as lived material----biology.In the research of life sciences, the research of " life electric wave " is more even more important than the research of the human body in sum! Certainly the research of " life electric wave " must just can be a complete biological study in conjunction with the human body.
Nearly 200 years is the epoch of chemical technology high development, particularly after 20th century since 70 years chemistry, biochemistry, biological engineering etc. the development of advancing by leaps and bounds has been arranged, thereby created ten hundreds of chemical productss.Instrument, the clothes of dress, edible food, ornamental cosmetics and the construction material etc. that use from the mankind all are chemical productss; Chemicals makes traditional agricultural become chemical agricultural, the fertilizer that no matter is to use, or diseases prevention, parasite killing, weeding all are chemical substances; For obtain high yield, what stimulate crop to grow fast to use also is chemical substance.Medically, particularly occupy 100% market at western countries' chemicals, also captured nearly 82% market in China.Various chemicalses such as a large amount of antibiotic, hormone, interferon are ubiquitous.The world of today no matter is in the family of oneself or bustling each corner, the world is that the tinkling of jades chemistry that meets the eye on every side is brilliant everywhere.Many in recent years scientists think that chemical industry is the human development that brings height, but has also brought healthy harm to the mankind simultaneously.This is because a large amount of chemicals has caused the pollution of environment, no matter is human edible food, and the water of still drinking all is subjected to the brilliant pollution of chemistry to some extent; A large amount of chemicalses, particularly antibiotics, interferons, hormone medicine endanger bigger; Even never use antibiotic, even the people who never takes medicine also can't avoid the harm of the entrained chemical substance of agricultural product, meat, egg, milk and their goods to health.The mankind have absorbed a large amount of chemical substances, have caused queer difficult and complicated illness to cure.This is because various chemical substances have caused human immune system's damage, reduction even disappearance.A large amount of in addition various chemical substances have been brought out various pathogenic microorganisms to the pollution of environment.From various in the world reports in recent years as can be known, no matter current pathogenic microorganism on kind, still all is greatly improved from pathogenecity.Therefore caused human diseases more and more, more and more stranger, more and more refractory is treated.Particularly diabetes are gone back neither one so far and are effectively cured way.Make a general survey of research institution in the world, a large amount of scientists is engaged in the research of medical science, national governments have dropped into the research of countless financial support medical science, many chemicalses are introduced to the market, yet not only traditional disease is not effectively controlled, newly produced various strange difficult miscellaneous diseases on the contrary, that does not stop challenges to medical circle! Produce in succession as acquired immune deficiency syndrome (AIDS), bovine spongiform encephalopathy etc., and medical circle felt simply helpless!
In sum, develop down according to present in the world medical research direction and method, not only diabetes can not get effectively prevention and treatment, also can bring out on the contrary more, upgrade, more refractory disease.How finding the way of diseases such as effective, safe control and treatment diabetes is the too impatient to wait major issues in the world today.
Purpose of the present invention provides a kind of specific plasma environmental modified processing yeast cells of usefulness to have the method for the biological preparation medicine of prevention and treatment diabetes with manufacturing for addressing the above problem.
Another object of the present invention provides the biological preparation medicine that obtains with said method.
The environmental modified technology of using plasma of the present invention activates yeast " latent function gene ", makes these yeast become the specific yeast with human activin immune cell function.Cultivate through the domestication under the plasma environmental modified condition of the present invention again, make the biological preparation medicine of prevention and treatment diabetes then.
Why the present invention can successfully obtain to be used for the biological preparation medicine of diabetes control, is that the present invention has adopted and the diverse method of conventional biotechnology.
Show that by a large amount of experiments biological preparation medicine of the present invention has following characteristic:
1. the environmental modified method of using plasma is different fully with the traditional life scientific research;
2. biological preparation medicine of the present invention is not a Chinese medicine, neither Western medicine, and more or not antibiotics, interferons, hormones etc., but a kind of biological preparation medicine of safe without toxic side effect.
Fig. 1 is the sketch map of gene structure.
Fig. 2 is that artificial plasma environment activates yeast " latent function gene " method sketch map.
Fig. 3 is the sketch map that activates back specific yeast amplification culture technology.
Fig. 4 is the sketch map of specific yeast liquid concentration technique.
Below in conjunction with accompanying drawing, feature of the present invention is described in further detail.
Consult Fig. 1, Fig. 1 illustrates the mechanism of this biological preparation medicine.Diabetes are formidable enemies of human health, and a large amount of studies show that, are that what disease is all relevant with the immunity of body.Various diseases can not take place when the immunity of body is strong.Now, various chemical substances, various antibiotic, hormone etc. cause the immunity of organisms cells injury, the immunocyte metabolism disorder, and immunogene can not be expressed normally, causes the immunologic function decline.The body of immunity recession is easy to be subjected to the invasion and attack of various pathogen, also can be subjected to the harm of various noxious substance, thereby causes the generation of diabetes.The decline of immunity of organisms is to be difficult to find according to the detection method of routine.Because it is normal that the conventional method machines is known from experience immunocyte quantity such as the intravital B of discovery machine, T, K, NK.Research of the present invention thinks whether the quantity of immunocyte can only be one of body's immunity index normally, but does not represent that the immunogene expression is normal, can not indicate that more immunoenzymatic character, activity also are normal.What of B, T, K, NK cell quantity are the immunologic function of immunocyte should not be in other words, will recognize also whether the expressed immunoenzymatic character of immunogene in these immunocytes is normal, and whether immunoenzymatic catalytic activity is enough.It is considered herein that, not only quantity is sufficient for the expressed immunoenzyme of immunogene, and enough catalytic effects must be arranged, that is to say, when body B, T, K, NK cell quantity just often, expressed immunoenzyme quantity might not capacity, when immunoenzyme quantity is enough, also good catalytic activity might not be arranged.Immunogene is the same with other genes, is divided into the two large divisions according to its effect, and Fig. 1 illustrates, and the left part of figure is the promoter part of immunogene, comprising promotor gene.Promotor gene is being undertaken the functions such as time that start the immunoenzymatic quantity of right side functional structure gene expression, expression.The right side of figure is called the functional structure Gene Partial, wherein mainly is structural gene.Structural gene has determined the structure of the expressed enzyme of this gene, it is enzymatic property, that is to say as long as the coiled structure of structural gene base sequence and gene strand is constant, its expressed enzymatic structure and character just can not change, but quantity that it is expressed and time, when express the enzyme amount few, when express the enzyme amount more, when express enzyme to summit etc., be subjected to the control of promotor gene fully.When the promoter of the immunogene of B, T, K, NK immunocyte partly is subjected to the influence of various injurious factors, when causing abnormal expression, directly have influence on the expressed immunoenzyme quantity of functional structure gene, expression time curve.When being subjected to extrinsic factor, the functional structure Gene Partial of immunogene disturbs, when causing its base or curly form to change, can influence expressed immunoenzymatic character of functional structure gene or activity, the immunity of body is seriously damaged, but conventional detection and be not easy discovery.In addition when the expressed immunoenzyme quantity of immunogene, when character is constant, immunogene influences injurious factor, whether also be crucial, Here it is said immunogene responsibility if making time of expression response consistent.When the responsibility of immunogene descends, or response time in advance or the decline that all can show immunity when lagging behind, and causes body ill.Why the B of body, T, K, does the immunogene of NK cell have the problems referred to above? of the present inventionly discover many anion or cationic " free radicals " of having, the endotoxin material that various pathogenic microorganism produced, and multiple neurological disorder, various radiation sources etc. all may cause B, T, K, NK immunocyte genovariation or " ossifing ", to this rigid gene, be referred to as " recessive gene " in the present invention, this recessive gene can not be timely, express accurately, pathogenic microorganism and multiple virulence factor can not be resisted timely to external world, cause body ill, comprise and suffered from diabetes.
Years of researches of the present invention are found, various immunogenes are in vital movement process separately, one species specific " life electric wave " launched in the capital, " life electric wave " difference that different immunogenes is launched, immunogene is made immunoreation by these " life electric waves " transmission immunologic information to the injurious factor of infringement body.Therefore when immunogene changed, " the life electric wave " launched will change, and that I guess a kind of small variation all can cause the variation of " life electric wave ".Key of the present invention has been to find that gene " life electric wave " can cause change because of injurious factor, but also can under the regulation and control that have useful electric wave material, recover normal, caused " recessive gene " of " ossifing " by injurious factor, can use useful factor activator.The frequency range that can be used for the electric wave of plasma environment of the present invention is 1800MHz-56000MHz, the 7000-11500MHz of preferred 3500-36000MH～more preferably.The intensity of electric wave is 115-445mv/cm.
The present invention adopts the artificial electric wave of the plasma environment of simulation body immunogene " life electric wave " according to above principle, create a kind of " the useful factor ", and this useful factor material made biological preparation medicine, use the immunogene of this biological preparation medicine regulation and control variation, thereby reach prevention and the effect of treatment diabetes.By the regulation and control immunologic function, make body strengthen the ability of resisting disease, make the fast quick-recovery of body of suffering from multiple sufferer.
The present invention thinks by a large amount of research, obtain a kind of active substance that can regulate and control immunogene by manual simulation " life electric wave ", is a very difficult job.The present invention only is " a drop in the ocean " through human use's in the ubiquitous microorganism of the exploration discovery nature in more than 20 years, this be because people to the microbial gene function solve few.Even to this day, the microorganism that full gene composition and function are understood by human institute only has 26 kinds, and these 26 kinds of microorganisms also only are kinds simple in structure, does not also understand for the eukaryotic microorganisms mankind of complexity.In the particularly human yeast microorganism that often uses, contain the gene that is not utilized in a large number, these genes have become " gene ossifys " owing to can not get for a long time using.The present invention claims that these " gene ossifys " are " latent function gene ".What is more important exists required for the present invention wanting in these " latent function genes ", can be used in the useful protein matter of regulation and control human body immunologic function.The present invention utilize the method for the manual simulation life electric wave that plasma environment produces activate in the yeast can expression regulation body's immunity protein substance " latent function gene " realize.
Using plasma environmental simulation life electric wave method of the present invention activates yeast " latent function gene ", cultivated 4 kinds of specific yeasts that activate B, T, K, 4 kinds of immunocytes of NK respectively, thus the biological preparation medicine of having made the prevention of regulation and control body's immunity and having cured diabetes.The present invention passes through stomach in order to make this 4 species specific yeast smoothly in edible back, and guarantees can not killed by a large amount of acidic materials under one's belt, and the present invention has done this 4 species specificity yeast the condition of anti-pH≤2.5 again and cultivated.Yeast cells after the cultivation will arrive small intestinal by the stomach organ smoothly.Under the effect of small intestinal plurality of enzymes, the specific yeast cell is cleaved, discharges to be packaged in intracellular specific immune function regulatory enzyme (albumen).The expression of immunogene in the activation of these specific immune function regulatory enzymes " specificity ", the corresponding immunocyte of regulation and control body.4 kinds of immunity of organism adjusting specific yeasts involved in the present invention owing to they all are the long-term edible or upward microorganisms of use of production of the mankind, can not produce any toxic and side effects.The organized enzyme that discharges in these specific yeast cells, have the moment activation, regulate the effect that the interior immunogene of body is correct, efficiently express, will be absorbed along with the intravital metabolism of machine loses the nutrient substance that activity is transformed into body very soon then, residual in can not causing in body.
The biological preparation mechanism of action brief description of regulation and control body's immunity involved in the present invention and prevention and treatment diabetes is as follows: the nucleus of regulation and control body's immunity biological preparation is 4 kinds and has the albumen that activates body B, T, K, NK " latent immunity gene " respectively that these 4 kinds of albumen contain respectively again in 4 species specificity yeast.This 4 primary yeast is exactly that the present invention's employing produces the activated microorganism of manual simulation " life electric wave " with plasma environment, claims that in the present invention these the 4 kinds yeast that simulated " life electric wave " activation " latent function gene " are specific yeast.Contain the immunoloregulation function enzyme that is activated respectively in this 4 species specificity yeast cells, these immunoloregulation function enzyme specificity ground activate immune B cell in the body, immune t-cell, immune t-cell and the recovery of immune NK cellular immunization gene, activation, and these immunogenes are correctly efficiently expressed.These specific yeast are cultivated out by specific simulation separately " life electric wave " condition.Activation body B cellular immune function specific yeast used in the present invention, employed manual simulation wave frequency and received-signal strength are by the decision of the immunogene specificity " life electric wave " of immune B cell; Activate body T cellular immune function specific yeast, employed manual simulation wave frequency and received-signal strength, be by the decision of the immunogene specificity " life electric wave " of immune t-cell: activate body K cellular immune function specific yeast, employed manual simulation wave frequency and received-signal strength are by the decision of the immunogene specificity " life electric wave " of immune K cell; Activate body NK cellular immune function specific yeast, employed manual simulation wave frequency and received-signal strength are by immunogene specificity " life electric wave " decision of immune NK cell.The specific yeast of these 4 kinds of difference in functionalitys activates, simulates " life electric wave " condition through recessive gene and cultivates, and makes and has produced the organized enzyme (albumen) that has activation, regulates and control above-mentioned 4 kinds of immunogenes in the cell.When using this 4 species specificity yeast preparation, specific yeast enters in the small intestinal of body, lysis under the effect of small intestinal plurality of enzymes, and the cell after the cracking discharges immunogene and activates the regulation and control enzyme.These immunogenes activate regulation and control enzymes and are entered blood by little intestinal absorption immediately, are transported to respectively in 4 kinds of immunocytes by blood, thereby reach activations, 4 kinds of immunogenes of regulation and control are correctly expressed raising immunity, thereby prevent and cured diabetes.
Immunologic function biological preparation medicine of the present invention is that the step by following two aspects realizes.First step is that the using plasma environment produces specific simulation " life electric wave " and activates common yeast, these yeast is become have the specific yeast of regulating B, T, K, NK cellular immune function, and these yeast are made anti-low pH condition cultivate; Second step is to produce under the condition of special simulation life electric wave at the using plasma environment, enlarges to cultivate these specific yeasts, thus the biologics of then these specific yeasts being made the prevention of regulation and control immunologic function and being cured diabetes.The implementation process of these two aspects is to realize by following step.
One. activate yeast function " recessive gene "
In above narration, illustrated that using plasma environment of the present invention produces simulation " life electric wave " method and activates common yeast, make latent function gene resurrection in the yeast, give expression to the albumen that has activation, regulates and control B cellular immunization gene, T cellular immunization gene, K cellular immunization gene and NK cellular immunization gene function respectively.As everyone knows, common yeast is the zymocyte of multiple materials such as class fermentation starch, saccharide, protide, be used to brew alcoholic beverages, make bread more, make various food, make the strain of medicine and multiple product thereof, though it is saccharomycetic various in style, function is different, but never the people utilizes the expressed specific proteins of yeast, activate, regulate B, T, K, NK immunogene function respectively, to carry out recessive gene in the method for not using patent of the present invention be not possess above-mentioned functions before activating to these yeast cells certainly.
(1) activate the method step that is used to regulate and control B cellular immune function gene yeast " latent function gene ":
A large amount of gene technology studies have shown that a complete B cellular immune function gene is formed (see figure 1) by the two large divisions, and a part is the promotor gene of B cellular immune function gene, and another part is the structural gene of B cellular immune function gene.The structural gene of B cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and the immunne response ability of the promotor gene control B cellular immune function gene of B cellular immune function gene, promptly immunoenzymatic tiring.Therefore, keep the character of the expressed enzyme of B cellular immune function gene constant, must manage to keep the structural gene DNA sequence of B cellular immune function gene constant; The structural gene that reaches B cellular immune function gene efficiently expresses, and keeps best immunocompetence of expressed immunoenzyme and immunne response ability timely, must manage to make the promotor gene of B cellular immune function gene efficiently to start.The present invention activates yeast " latent function gene " by manual simulation " life electric wave " method, obtains to have the specific yeast of expression regulation B cellular immune function gene protein.
Consult Fig. 2, the method step of using plasma environment generation manual simulation involved in the present invention " life electric wave " activation yeast " latent function gene " is as follows:
1. according to the one-tenth assignment system culture medium of table 1, and sterilization treatment.
2. select suitable yeast specie (can select yeast to see Table 3), according to active yeast cell/culture medium more than or equal the ratio of 100,000,000/1000ml, inject the culture medium in the container culture dish shown in Figure 2 (34).
3. keep container culture dish (34) temperature between 37 ± 5 ℃, cultivated 24-56 hour earlier.
4. open plasma generator shown in Figure 2, electrion pin (31) output wave frequency is adjusted in the 7200-10700MHz scope.
5. coil bag (35) output electromagnetic field intensity is adjusted to: in the 115-445mv/cm scope.
6. the culture dish (34) of Yeast Cultivation liquid will be housed, install in the plasma generator according to pattern shown in Figure 2.
7. under these conditions, and keep 37 ± 5 ℃ temperature conditions, activate 42-72 hour.
8. activate the back yeast cells through above condition, adopt vacuum freezing drying method to make ampoule then or make the powder preservation.
T, the method and the step of K.NK cellular immune function gene regulation, except that the frequency of utilization difference, its method step is identical.
Consult Fig. 2, artificial plasma device (3) activates zymic being described in detail as follows: the strain of yeast shown in Fig. 2 (1) is disposed modification and is activated the key diagram of yeast " latent function gene " in the plasma environment that a generation plasma device (3) is produced.Plasma state is that a large amount of molecular atoms lose that electronics becomes cation and trapped electron becomes the formed state of anion, can be formed by electrion under coarse vacuum by gas usually, and its state can utilize the Mike's Si dimension equation in the electromagnetism roughly to determine.Shown in the figure, plasma (2) is in the inner shell (33) that is limited in plasma device (3), the fan door is set to put into and to take out yeast strain (1) and corresponding yeast auxotype culture medium on the inner shell (33), the fan door can make inner shell (33) sealing when closed, place culture dish (34) in the inner shell (33), place yeast strain (1) and corresponding yeast auxotype culture medium in the culture dish (34)
Side at inner shell (33) inwall is provided with one to several electrion pins (31), and electrion pin (31) can be coupled with the high voltage of thousands of volts to tens thousand of volts, thereby makes electrion pin (31) produce point discharge, makes gas ionization, produces plasma.The electrion pin (31) of inner shell (33) inwall other be provided with one to several air inlet pipe (32) to inner shell (33) in, to inflate, can fill respectively with noble gas helium neon and so on for example, or nitrogen, and easily ionizable gas such as hydrogen etc.That adopts is ionized gas multiple choices can be arranged, can select as required, also can utilize the gas of Organic substance and petrochemicals to be charged into to form required plasma environment, opposite side at inner shell (33) inwall is provided with one to several plate shape electrodes (36), match with electrion pin (31), produce required electric field.Appropriate location is provided with vacuum-pumping tube (37) on inner shell (33) inwall, and it links to each other with the vacuum pump of outside evacuation, so that required vacuum is pumped in the space in the inner shell (33).In inner shell (33) inboard or arranged outside produce the coil bag (35) in magnetic field, make the plasma environment in the inner shell (33) be subjected to the action of a magnetic field of coil bag (35), for example, coil bag (35) produces magnetic field of thousands of Gausses and so on, like this, plasma environment in the inner shell (33) can be by the independent or synergy of applied field and magnetic field institute, plasma generation is moved and rotate, by preset program yeast strain (1) is carried out modification and handle and activate yeast " latent function gene ", processing time can be determined as required, can be a few hours, tens of hours, so that hundreds of hours.
Two. the domestication of specific yeast acidproof (anti-low pH)
Illustrated that more than using plasma environment of the present invention produces the method for manual simulation " life electric wave ", activate yeast difference " latent function gene " respectively, obtain 4 kinds of specific yeasts that are respectively applied for activation, regulation and control B cellular immunization gene, T cellular immunization gene, K cellular immunization gene and NK cellular immunization gene function.But this 4 species specificity yeast can't be directly used in the various immunogenes of regulation and control, and this is because they can't the intravital environment of adaline.As everyone knows, be a very complex environment in the body, and various envirment factor is all changing all the time.When this 4 species specific yeast enters the approach of small intestinal by oral cavity, stomach, be difficult to ensure its cell integrity, therefore the activity of purpose enzyme in the more difficult guarantee yeast cells ensures that the yeast cells activity is very crucial.The present invention has adopted the zymic envirment factor domestication of 4 species specificity cultivation, and the domestication and culture method step is as follows:
The domestication of the environmental suitability of 4 kinds of functional microorganisms of the present invention is that the method by as shown in Figure 2 realizes:
Consult Fig. 2, the method step of the present invention's 4 species specificity yeast environmental suitabilities domestication is as follows respectively:
(1) the specific yeast step of domestication regulation and control B cellular immunization gene
1. the method according to table 2 configures culture medium, and sterilization treatment.
2. get the container culture dish (34) of the culture medium 1000ml injection Fig. 2 in the step 1.
3. get regulation and control B cell specific yeast liquid 10ml (yeast mixture living cells content more than or equal 100,000,000/ml), inject container culture dish (34) shown in Figure 2,
4. open plasma generator as shown in Figure 2, and be adjusted on the specific yeast specificity frequency 7200-10700MHz of regulation and control B cellular immunization gene,
5. the electric wave output voltage of regulating as shown in Figure 2 is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ standby.
Three, the method for making of biological preparation medicine of the present invention
Specific yeast involved in the present invention only is to have obtained seed after obtaining through said method, make a large amount of biological preparation medicines of the present invention, and the specific yeast of capacity must be arranged, and therefore needs amplification culture.It is that step realizes by the following method that specific yeast is cultivated:
The specific yeast culture process of A, adjusting B cellular immunization gene function
The specific yeast culture process engineering of adjusting B cellular immune function involved in the present invention as shown in Figure 3.
Consult description Fig. 3 of the present invention, the specific yeast culture process step of adjusting B cellular immune function involved in the present invention is as follows:
1. according to the one-tenth assignment system culture medium of table 4, and after sterilization treatment, be injected into respectively in A, B, the C jar of Fig. 3.
2. will activate through artificial plasma environment simulation life electric wave method, and the specific yeast of the adjusting B cellular immune function that cultivation obtained through tolerating low pH (less than pH2.5), be input among the seed tank A shown in Figure 3, as seed liquor.A jar seed liquor is injected into amplification culture in the culture medium of B jar according to certain ratio then, the ratio of injection is: A seed liquor/B culture fluid=5ml/1000ml,
3. adjusting wave generator, making it export biological electric wave is 7200-10700MHz, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity as culture fluid in the hypothesis B jar is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).
4. keep under the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ of conditions.
5. when the specific yeast living cells of regulating B cellular immunization gene in the B jar reaches 2,000,000,000/ml, in B jar yeast mixture input C jar, prepare to be transported to down road technology.
T, the acclimation method step of K.NK cell is identical with above-mentioned steps, the frequency difference that only is to use.
Below will specifically describe embodiment of the present invention by specific embodiment.Though only relate to a kind of concrete yeast in each embodiment, the inventor finds also can obtain identical result with other yeast bacterial strain by experiment.
Embodiment 1: activate the method that is used to regulate and control B cellular immune function gene yeast " latent function gene ":
1. according to the one-tenth assignment system culture medium 1000-2000ml of table 1, and sterilization treatment.
2. select IFFI 1021 yeast species, according to live IFFI 1021 cells/culture medium more than or equal the ratio of 100,000,000/1000ml, inject the culture medium in the container culture dish shown in Figure 2 (34).
3. keep container culture dish (34) temperature between 37 ± 5 ℃, cultivated 24-56 hour earlier.
4. open plasma generator shown in Figure 2 (3), electrion pin (31) output wave frequency is adjusted in the 7200-10700MHz scope.
5. line bag circle (35) output electromagnetic field intensity is adjusted to: in the 11.5-44.5V scope.
6. the culture dish (34) of yeast IFFl1021 culture fluid will be housed, install in the plasma generator according to pattern shown in Figure 2.
7. under these conditions, and keep 37 ± 5 ℃ temperature conditions, activate 42-72 hour.
8. activate back IFFl1021 yeast cells through above condition, adopt vacuum freezing drying method to make ampoule or make the powder preservation.
Embodiment 2. immunomodulators are to the inhibition effect of 180 ascites tumors
A. get 60 of wates rats, be divided into totally 3 groups of A, B, C, every group of 20 rats.The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt 180 ascites tumors, rat is respectively organized in inoculation respectively.
C. from inoculating back second day, A organize to this biological preparation liquid (the specific yeast cell more than or equal 100,000,000/ml), dosage 0.6ml/kg; B organizes to cyclophosphamide, and dosage is that 20ug/kg:C organizes to normal saline 0.6ml/kg.Once a day.
D. dissect to detect the size of ascites tumor after seven days, as following table:
| The data group | Tumor is heavy | Ratio % | Explanation |
| The C group | 22g ± 4.2/ | 100% (as 100%) | Meansigma methods |
| The B group | 16g ± 3.7/ | ??-27.2％ | Meansigma methods |
| The A group | 3g ± 0.3/ | ??-86.4％ | Meansigma methods |
Embodiment 3. immunomodulators are to the inhibition effect of U-14 solid tumor
A. get 90 of wates rats, be divided into totally 3 groups of A, B, C, every group of 30 rats.The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt the U-14 solid tumor, rat is respectively organized in inoculation respectively.
C. from postvaccinal second day, A organize to this biological preparation liquid (the specific yeast cell more than or equal 100,000,000/ml), dosage is that 0.6ml/kg:B organizes to cyclophosphamide, dosage is 20ug/kg; C organizes to normal saline 0.6ml/kg.Once a day.
D. take after 14 days and to dissect the size that detects ascites tumor, as following table:
| The data group | Tumor is heavy | Ratio % | Explanation |
| The C group | 19g ± 2.2/ | 100% (as 100%) | Meansigma methods |
| The B group | 17g ± 1.6/ | ??-10.5％ | Meansigma methods |
| The A group | 2g ± 0.2/ | ??-89.5％ | Meansigma methods |
This microorganism formulation is by the oncotherapy of mouse has been obtained good curative effect, and is same, also quite effective to human body prevention and treatment diabetes.
Consult Fig. 4, shown is that the yeast liquid of the adjustable various immunogenes after the domestication is a kind of by prevention and the most effective formulation selection of treatment diabetes, two kinds, three kinds, or the yeast liquid of four kinds above-mentioned immunogene, by after clinical selected certain proportion cooperates, mixing in advance, carry out spissated key diagram more again, can carry out twice and concentrate, to reach the suitable concentration of being convenient to encapsulate.
The biological preparation medicine of prevention after the described step of Fig. 4 and treatment diabetes is concentrated cooling, and weighing, potting go out finished product, have just made the biological preparation medicine that is used to prevent and cure diabetes.It is the microbial medicine of producing through the plasma ambient modification technology.
Table 1. activates regulation and control (B, T, K, NK) cell used yeast " latent function gene " medium component table
| Medium component | Quantity |
| Mannitol | ??16g |
| ??K 2HPO 4 | ??0.25g |
| ??MgSO 4.7H 2O | ??0.2g |
| ??NaCl | ??0.22g |
| ??CaSO 4·H 2O | ??0.5g |
| ??CaSO 4·H 2O | ??0.5g |
| ??CaCO 3 | ??6.0g |
| ??Urea | ??0.2-0.5g |
| Serum | ??100--300ml |
| Distilled water | ??700--900mL |
Table 2. environmental condition adaptability medium component table (is example with 1000ml)
| Medium component | Quantity | Explanation |
| Sucus Ziziphi Spinosae | ????300ml | With the clear liquid that dry acid Fructus Jujubae/water=the 1g/5ml ratio is made |
| Malus baccata juice | ????500ml | With the clear liquid that dried Malus baccata/water=the 1g/5ml ratio is made |
| ??(NH 4)SO 4 | ????0.25g | |
| ??K 2HPO 4 | ????0.2g | |
| ??MgSO 4·7H 2O | ????0.22g | |
| ??NaCl | ????0.5g | |
| ??CaSO 4·2H 2O | ????0.3g | |
| ??CaCO 3 | ????3.0g | |
| Specific yeast culture fluid after the activation | Each 20ml | Contain active yeast cell more than or equal 100,000,000/ml |
The microbe species (showing listed microorganism) that table 3. this patent is related but be not limited only to this
Saccharomyces cerevisiae, ellipse brewing yeast, Xue's watt yeast, Dare cloth yeast, Mongolia's Dare cloth yeast, saccharomyces exiguus, saccharomyces fermentati, Lip river lattice yeast, saccharomyces mellis, little saccharomyces ellipsoideus, ellipsoideus yeast, the Luo Si yeast, the Lu Shi yeast, saccharomyces sake, Candida arborea, bright Bick candida mycoderma, this yeast of Crewe, Candida lipolytica, medium-sized level and smooth candida mycoderma, Candida parapsilosis, the iron oxide red yeast, fold candida, candida tropicalis, Candida utilis, the false capsule yeast of A Shu, geotrichum candidum, unusual Hansenula yeast, the 1,2,3,4,5-pentanepentol Hansenula yeast, outstanding fourth Hansenula yeast, Saturn Hansenula yeast, the Amur Hansenula yeast, inferior film Hansenula yeast, Fructus Citri Limoniae shape Kloeckera japonica, saccharomyces oleaginosus, pichia farinose, Pichia membranefaciens Hansen, red winter spore yeast, Rhodothece glutinis, little Rhodothece glutinis, rhodothece rubra, the Ka Ersi yeast, Saccharomyces uvarum, the Weir yeast, rood class yeast, China's class yeast, eight spore fission yeasts, chestnut wine fission yeast, shadow yeast, Torulopsis candida, unknown torulopsis, torulopsis, usual torulopsis, shellfish thunder trichosporon, the head trichosporon, skin shape trichosporon, the Brunswick yeast.
Table 4. specific yeast amplification culture based component table (in the 1000L culture fluid)
| Medium component | Quantity |
| Haw liquid | ??200L |
| Fructus Schisandrae Chinensis liquid | ??200L |
| Fructus Jujubae liquid | ??200L |
| Soybean juice | ??200L |
| Fructus Mali pumilae liquid | ??200L |
Annotate: 1. in the table various liquid all be according to: the ratio of material/water=1/10 is processed into.
2. going up the table culture fluid will adjust in pH2.5 ± 0.2 scope.
Claims (10)
1. make the method for biological preparation, it is characterized in that, use the radio wave of the characteristic frequency of plasma environment to handle yeast cells with prevention and treatment diabetes.
2. the method for claim 1 is characterized in that, handles saccharomycetic plasma environment and can be subjected to electric field and the action of a magnetic field.
3. the method for claim 1 is characterized in that, yeast processed time in plasma environment can be a few hours, tens of hours, so that hundreds of hours.
4. method as claimed in claim 1, wherein the frequency of electric wave is 7200-10700MHz.
5. method as claimed in claim 1, wherein the intensity of electric wave is 115-445mv/cm.
6. method as claimed in claim 1, yeast cells wherein is selected from: saccharomyces cerevisiae, ellipse brewing yeast, Xue's watt yeast, Dare cloth yeast, Mongolia's Dare cloth yeast, saccharomyces exiguus, saccharomyces fermentati, Lip river lattice yeast, saccharomyces mellis, little saccharomyces ellipsoideus, ellipsoideus yeast, the Luo Si yeast, the Lu Shi yeast, saccharomyces sake, Candida arborea, bright Bick candida mycoderma, this yeast of Crewe, Candida lipolytica, medium-sized level and smooth candida mycoderma, Candida parapsilosis, the iron oxide red yeast, fold candida, candida tropicalis, Candida utilis, the false capsule yeast of A Shu, geotrichum candidum, unusual Hansenula yeast, the 1,2,3,4,5-pentanepentol Hansenula yeast, outstanding fourth Hansenula yeast, Saturn Hansenula yeast, the Amur Hansenula yeast, inferior film Hansenula yeast, Fructus Citri Limoniae shape Kloeckera japonica, saccharomyces oleaginosus, pichia farinose, Pichia membranefaciens Hansen, red winter spore yeast, Rhodothece glutinis, little Rhodothece glutinis, rhodothece rubra, the Ka Ersi yeast, Saccharomyces uvarum, the Weir yeast, rood class yeast, China's class yeast, eight spore fission yeasts, chestnut wine fission yeast, shadow yeast, Torulopsis candida, unknown torulopsis, torulopsis, usual torulopsis, shellfish thunder trichosporon, the head trichosporon, skin shape trichosporon, the Brunswick yeast.
7. method as claimed in claim 6, yeast cells wherein are saccharomyces cerevisiae IFFI1021.
8. the yeast cells that makes with the method for arbitrary claim among the claim 1-7.
9. a biological preparation medicine that is used to prevent and cure diabetes is characterized in that, the yeast cells that contains claim 8 is as active component.
10. yeast cells as claimed in claim 8 is used to prevent and cure the purposes of diabetes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031136176A CN1519031A (en) | 2003-01-21 | 2003-01-21 | Biological preparation modified in plasma environment for treating diabetes and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031136176A CN1519031A (en) | 2003-01-21 | 2003-01-21 | Biological preparation modified in plasma environment for treating diabetes and preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1519031A true CN1519031A (en) | 2004-08-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031136176A Pending CN1519031A (en) | 2003-01-21 | 2003-01-21 | Biological preparation modified in plasma environment for treating diabetes and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1519031A (en) |
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2003
- 2003-01-21 CN CNA031136176A patent/CN1519031A/en active Pending
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