CN1380100A - Biological immunomodulator for animal for curing and preventing foot and mouth disease and its production method - Google Patents
Biological immunomodulator for animal for curing and preventing foot and mouth disease and its production method Download PDFInfo
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- CN1380100A CN1380100A CN 01110635 CN01110635A CN1380100A CN 1380100 A CN1380100 A CN 1380100A CN 01110635 CN01110635 CN 01110635 CN 01110635 A CN01110635 A CN 01110635A CN 1380100 A CN1380100 A CN 1380100A
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Abstract
The present invention relates to a biological immune regulator for preventing and curing foot and mouth disease of animals. It adopts the Micro-Alternating-field Biotechnology, called MAB for short, and uses yeast to make preparation, it can activate some corresponding cellular immune cryptomorphic functional gene in yeast, and makes the recessive gene of its structure gene portion be activated under the condition of no changing DNA sequence to implement high-effective expression of immunological function so as to obtain the injection and oral preparation of said biological immune regulator for preventing and curing foot and mouth disease.
Description
The present invention relates to biologic product, particularly adopt micro-alternating bio-electric field (Micro-Alternating-field Biotechnology is called for short MAB) modulation radio ripple to produce the livestock biologic product that is used for prevention and cures aftosa.
At present, in Europe and some other place, the world and aftosa has appearred, these have been suffered from the livestock of aftosa, carried out mostly that killing disappears ruins, in case the stretching of seam fever aphthous, but can prevent or cure aftosa without any medicine or immunomodulator at present, the livestock that got aftosa can be infected, thereby the livestock of prevention and treatment aftosa etc. extremely needs with biological immunomodulator.
Purpose of the present invention is to provide a kind of livestock manufacture method of biological immunomodulator of preventing and/or curing aftosa, and this kind of producing with the method biological immunomodulator is provided.
The object of the present invention is achieved like this, adopt a kind of like this production method of producing biological immunomodulator in micro-alternating bio-electric field (MAB), this biological immunomodulator is applicable to various livestocks, be used for prevention and device and/or cure aftosa, it is characterized in that, adopt barms, and adopt following key step:
Adopt a kind of like this manufacture method that saccharomycete is made into the livestock usefulness immunomodulator with specific immunologic function, for giving anti-and curing various aftosas, it is characterized in that, the characteristic radio magnetic wave of employing micro-alternating bio-electric field activates the step of the latent function gene of every Yeasts, includes:
1. gene activation device (2) is set,
2. according to one-tenth assignment system culture medium first (3) P of subordinate list one, and sterilization treatment,
3. select the saccharomyces cerevisiae of IFFI1021 yeast specie, according to IFFI1021 wine brewing cell/culture medium 〉=1 * 10 of living8Individual ratio, inject the culture medium first, to cultivate again the gene activation case (23) that first (3) is put into gene activation device (2), as mentioned above, why is biotechnology research in this unfavorable situation? it is considered herein that key is a method problem. What conventional biotechnology research adopted is the methods such as biochemistry, biomolecular science, and these methods all are to be come by the chemical method development of routine. Chemical method is to study the variations such as the chemical combination of non-living matter, decomposition, redox basically. Biology then is lived material, and the per minute per second was all ceaselessly changing work when these living matters were per, and older generation's demutation of new generation of dying is alternately constant. Only be the metabolism of material in the least unit-cell that forms human body, orderly the showing of conversion of material, never can stop. Current biotechnology research all is actually very incomplete to this tangible material of the human body as life. Research of the present invention is thought, the most basic should being formed by the two large divisions of lived organism, a part be visible, touch the human body to get work, i.e. tangible material; Another part is that conventional theory is thought invisible material-" electric field " that cannot see, touch not work. Here " electric field " of indication is " the life electric wave " that is present on each organism. On the life entity of a work, this life electric wave is ceaselessly launched, and after this invisible " life electric wave " lost, life had also just stopped, and the human body also just becomes dead material-dead body (human body). All researchs of the world today all are only to study dead material-human body, and never people's research " life electric wave ". The human body is a kind of dead material in a sense, and " life electric wave " is only material alive, and the human body is the host of " life electric wave " just, i.e. emission source. Only have when the human body and " life electric wave " organically combine and just can be referred to as the material-biology of life. In the research of life science, the research of " life electric wave " is more even more important than the research of the human body in sum! Certainly the research of " life electric wave " must just can be a complete biological study in conjunction with the human body.
Why the present invention can successfully obtain the immunomodulator biological products for the various diseases control, and key is to adopt and the diverse method of conventional biotechnology research. Research method involved in the present invention is the life true essence that combines organism, has both studied the human body characteristic of life entity, studies again life entity active material " life electric wave ". The present invention's research " life electric wave " can live with sensual necessary condition, and research " life electric wave " is to the regulation and control of the composition of host substance simultaneously. By the successful acquisition of this research the biological immunomodulator initiated of the world. Experiment shows, this immunomodulator has safely, has no side effect, effect is remarkable. So, can be used for prevention and treat the livestock of aftosa. The cause of disease of aftosa can be from following factor in advance to consider: recently be the epoch of chemical technology high development over 200 years, particularly chemical over 70 years after 20th century, biochemistry, bioengineering etc. have had the development of advancing by leaps and bounds. Thereby created several hundred million ten thousand chemical products, instrument, the clothes of wear, edible food, ornamental cosmetics and the construction material etc. that use from the mankind all are chemical products; Chemicals makes traditional agricultural become chemical farming, no matter is the fertilizer that uses, or diseases prevention, desinsection, weeding all are chemical substances; To use chemical substance to stimulate the crop Fast Growth also be the chemical substance of using in order to obtain high yield. Medically, particularly occupy 100% market in western countries, also captured nearly 82% market in China. The various chemicalses such as a large amount of antibiotic, hormone, interferon are ubiquitous. No matter the world of today is in the family that controls oneself or bustling each corner, the world, is the tinkling of jades chemicals that meets the eye on every side everywhere. Many scientists think in recent years, and chemical industry is the human development that brings height, but has also brought healthy harm to the mankind simultaneously. This is because a large amount of chemicals has caused the pollution of environment, no matter is the edible food such as the mankind or livestock, and the water of still drinking all is subject to the pollution of chemicals to some extent; A large amount of chemicalses, particularly antibiotics, interferons, hormone medicine endanger larger; Even never use antibiotic, even the people who never takes medicine and livestock birds and beasts also can't escape the chemical substance brought from agricultural product, meat, egg, milk and their goods to the harm of health. People and livestock etc. have absorbed a large amount of chemical substances, have caused queer difficult and complicated illness to cure. This is because various chemical substances have caused immune damage, reduction even the shortages such as human body and livestock. A large amount of various chemical substances are to the pollution of environment in addition, various pathogenic microorganisms have been brought out, from in recent years in the world various reports as can be known, current pathogenic microorganism or all is greatly improved from pathogenecity no matter on kind. Therefore caused human diseases more and more, more and more stranger, more and more refractory is treated. Very general such as various tumours, hepatitis, diabetes, uremia etc., effectively cure way but go back even to this day neither one. Making a general survey of the various research institutions in the world is found everywhere, a large amount of scientists is engaged in the research of medical science, national governments have dropped into the research of countless financial support medical science, various chemicalses are introduced to the market, yet not only traditional disease is not effectively controlled, and has newly produced on the contrary the assorted disease of various strange difficulties and has ceaselessly challenged to medical circle! In succession put into effect such as AIDS, aftosa etc., medical circle is felt simply helpless!
Have data to show, the antibiotic that uses in the world today is kind more than 100 nearly, and hormone also has tens kinds, and various chemical substances are countless especially. These antibiotic, hormone not only use at human body, and the use on the industries such as livestock cultivation, poultry farming, aquaculture is more extensive, and become requisite composition in the feed, be that kind or quantity are all considerably beyond the use amount on the person. Therefore a large amount of antibiotic, hormone and the number of chemical medicines of residual work in meat, egg, milk and the goods that eat, indirectly enter the human body of torturing the work mankind and livestock in the people, animal body, with showing this antibiotic use, find that not only this antibiotic is no longer so efficacious, has brought out new disease, new pathogenic microorganism on the contrary. Cause various new difficult and complicated cases, such as: aftosa, H5N1 avian influenza virus etc. Various diseases are formidable enemies of livestock and human health, also are to cause livestock and human dead main factor. A large amount of studies show that, be which type of disease is all relevant with the immunity of human body. Various diseases can not occur when the immunity of human body is stronger. But cause the damage of body immunity cell with work various chemical substances, various antibiotic, hormone etc., the immunocyte metabolic disorder, immunogene can not be expressed normally, causes the decline of immunologic function. The human body of immunity decline is easy to be subject to the invasion and attack of various pathogen, also can be subject to the harm of various noxious material, thereby causes various diseases to occur. The decline of body immunity is to be difficult to find according to the detection method of routine. Because detecting human body, conventional method can find that B, T, the immunocyte quantity such as K, NK in the human body are normal. Research of the present invention thinks whether the quantity of immunocyte can only be one of body immune function index normally, but does not represent that the immunogene expression is normal, can not indicate that more immunoenzymatic character, activity also are normal. What of B, T, K, NK cell quantity are the immunologic function of immunocyte should not be in other words, will recognize also whether the expressed immunoenzymatic character of immunogene in these immunocytes is normal, and whether immunoenzymatic catalytic activity is enough. It is considered herein that, not only quantity is sufficient for the expressed immuno-enzymatic of immunogene, and enough catalytic effects must be arranged, that is to say, as human body B, T, K, when the NK cell quantity is normal, expressed immuno-enzymatic quantity might not capacity, when immuno-enzymatic quantity is enough, also good catalytic activity might not be arranged. Immunogene is the same with other genes, is divided into two large divisions's (seeing accompanying drawing one) according to its effect, and left part is the promoter part of immunogene, comprising promotor gene. Promotor gene is undertaken the functions such as time that work starts the immunoenzymatic quantity of right side functional structure gene expression, expression. The right side is referred to as the functional structure Gene Partial, wherein mainly be structural gene, structural gene has determined the structure of the expressed enzyme of this gene, it is enzymatic property, that is to say as long as the coiled structure of structural gene Xian basic sequence and gene strand is constant, its expressed enzymatic structure and character just can not change, but its expressed quantity and expression time, when show the enzyme amount few, when express the enzyme amount more, when express enzyme and arrive top etc., the control of promotor gene on the left of being subjected to fully. When the promoter gene of the immunogene of B, T, K, NK immunocyte partly is subject to the impact of various injurious factors, when causing abnormal expression, directly have influence on the expressed immuno-enzymatic quantity of functional structure gene, expression time curve. When being subject to extrinsic factor, the functional structure Gene Partial of immunogene disturbs, when causing its Xian base or curly form to change, can affect the expressed immunoenzymatic character of functional structure gene or activity, the immunity of human body has been suffered heavy damage, but conventional detection and be not easy to find. In addition when the expressed immuno-enzymatic quantity of immunogene, when character is constant, immunogene affects injurious factor, whether make time of expression response consistent also is very important, Here it is said immunogene responsibility, responsibility decline when immunogene, or response time in advance or the decline that all can show immunity when lagging behind, and causes human body ill. Does why the immunogene of B, the T of human body, K, NK cell have the problems referred to above? many endotoxin materials that produce with anion or cationic " free radical ", various pathogenic microorganism are found in research of the present invention, and multiple neurological disorder, various radiation sources etc. all may cause B, T, K, NK immunocyte genetic mutation or " ossifing ", to this rigid gene, be referred to as in the present invention " recessive gene ", this recessive gene can not be in time, express accurately, pathogenic microorganism and multiple virulence factor can not be resisted timely to external world, cause human body ill. The present invention finds through years of researches, various immunogenes are in vital movement process separately, one species specific " life electric wave " launched in the capital, " life electric wave " difference that different immunogenes is launched, immunogene is made immune response by these " life electric waves " transmission immunologic information to the injurious factor of infringement human body. Therefore when immunogene changed, " the life electric wave " launched will change, and that pipe is that a kind of small variation all can cause the variation of " life electric wave ". Key of the present invention has been to find that gene " life electric wave " can cause change because of injurious factor, but also can under with the regulation and control of useful electric wave material, recover normal, caused " recessive gene " of " ossifing " by injurious factor, can use useful factor activator.
The present invention is according to above principle, adopt the artificial electric wave of simulation human body immunogene " life electric wave ", create a kind of " the useful factor ", and this useful factor material made biologic product, regulate and control the immunogene of variation with this biologic product, thereby reach the effect of regulating immunologic function. By the regulation and control immunologic function, make human body strengthen the ability of resisting disease, make the fast quick-recovery of human body of suffering from multiple sufferer.
The present invention thinks by a large amount of research, obtain a kind of active material that can regulate and control immunogene by manual simulation's " life electric wave ", is a very difficult job. The present invention was through the exploration discovery in more than 20 years, and the microorganism human use's that occurring in nature is ubiquitous only is " one mao of nine livestock ", this be because people to the microbial gene function understand few. Even to this day, the human microorganism of understanding the full gene constitute and function only has 26 kinds, and these 26 kinds of microorganisms also only are kinds simple in structure, does not also understand for the eukaryotic microorganisms mankind of complexity. Particularly in the human yeast microorganism that often uses, contain the gene that is not utilized in a large number, these genes have become " gene ossifys " owing to can not get for a long time using, and the present invention claims that these " gene ossifys " are " latent function gene ". What is more important exists in these " latent function genes " that work is required for the present invention to be wanted, and can be used in the useful protein matter of the body immune function of the human and livestock of regulation and control. The present invention utilize the method for manual simulation's life electric wave activate in the yeast can expression regulation body immune function protein substance " latent function gene " realize.
The present invention adopts simulation life electric wave method to activate yeast " latent function gene ", has cultivated 4 kinds of specific yeasts that activate respectively B, T, K, 4 kinds of immunocytes of NK, has made the biologic product of regulation and control body immune functions. The present invention is in order to make this 4 species specific yeast, and is edible rear smoothly by stomach, and guarantees can not killed by a large amount of acidic materials under one's belt, and the present invention has done this 4 species specificity yeast again the condition of anti-PH≤2.5 and cultivated. Yeast cells after the cultivation will arrive small intestine by the stomach organ smoothly. The specific yeast cell is cleaved under the effect of small intestine plurality of enzymes, discharges to be packaged in intracellular specific immune function regulatory enzyme (albumen). The expression of immunogene in the activation of these specific immune function regulatory enzymes " selectivity ", the corresponding immunocyte of regulation and control human body. 4 kinds of human body immunological regulation specific yeasts involved in the present invention because they all are for a long time upper microorganisms of using of edible or production of human livestock, can not produce any toxic and side effect. The organized enzyme that discharges in these specific yeast cells, have the moment activation, regulate the effect that the interior immunogene of human body is correct, efficiently express, then will lose very soon the nutriment that activity is transformed into human body with the metabolism of work in the human body and be absorbed, residual in can not causing in human body.
The mechanism of action brief description of related regulation and control body immune function biologic product of the present invention is as follows: the nucleus in the regulation and control body immune function biologic product is 4 kinds and has the albumen that activates respectively human body B, T, K, NK " latent immunity gene " that these 4 kinds of albumen contain respectively again in 4 species specificity yeast. This 4 primary yeast is exactly the microorganism that the present invention adopts manual simulation's " life electric wave " to activate, and claims that in the present invention these the 4 kinds yeast that simulated " life electric wave " activation " latent function gene " are specific yeast. Contain respectively the immunoloregulation function enzyme that is activated in this 4 species specificity yeast cells, immune B cell, immune t-cell, immune K cell and immune NK cellular immunity gene recover, activate in the activation human body of these immunoloregulation function enzyme specificities, and these immunogenes are correctly efficiently expressed. These specific yeast are cultivated out by separately specific simulation " life electric wave " condition. Activation human body B cellular immune function specific yeast used in the present invention, employed manual simulation's wave frequency and received-signal strength are to be determined by the immunogene specificity " life electric wave " of immune B cell; Activate human body T cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune t-cell determines; Activate human body K cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune K cell determines; Activate human body NK cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life electric wave " by immune NK cell determines. The specific yeast of these 4 kinds of difference in functionalitys activates, simulates " life electric wave " condition through recessive gene and cultivates, and makes and has produced the organized enzyme (albumen) that has activation, regulates and control above-mentioned 4 kinds of immunogenes in the cell. When using this 4 species specificity yeast preparation, specific yeast enters in the small intestine of human body, lysis under the effect of small intestine plurality of enzymes, and the cell after the cracking discharges immunogene and activates the regulation and control enzyme. These immunogenes activate regulation and control enzymes and are entered blood by intestinal absorption immediately, are transported to respectively in 4 kinds of immunocytes by blood, thereby reach the purpose of activations, 4 kinds of immunogene corrections raisings of regulation and control immunity.
Immunologic function biological regulator of the present invention is that the step by following two aspects realizes. The step of first aspect is to adopt specific simulation " life electric wave " to activate common yeast, the specific yeast that these yeast is become have different adjustment B, T, K, NK cellular immune function, and these yeast are made anti-low PH condition cultivate; The step of second aspect is under the condition of special simulation " life electric wave ", enlarges to cultivate these specific yeasts, then these specific yeasts is made the biological products that the regulation and control immunologic function is used.
It is bacterial classification gene structure key diagram that this specification includes following accompanying drawing: Fig. 1. Fig. 2 is latent function gene activation device key diagram. Fig. 3 is that the wireless mode of latent function gene is laid key diagram. Fig. 4 is the step key diagram of the method that is activated of latent function gene. Fig. 5 is strain domestication device key diagram. Fig. 6 is the step key diagram of the bacterial classification method of being tamed. Fig. 7 will pass through the square frame key diagram of the bacterial classification of domestication to the step of the biological immunomodulator of making the oral agents form. Fig. 8 is that special yeast enlarges the culture process schematic diagram after activating. Fig. 9 is specific yeast liquid hybrid technique schematic diagram. Figure 10 is the concentrated key diagram of specific yeast liquid. Figure 11 is cooling metering and finished product packing schematic diagram.
Below in conjunction with accompanying drawing, each feature of method of the present invention is described in further detail. Consult Fig. 1, Fig. 1 is bacterial classification gene structure key diagram.
As previously mentioned, shown in the figure is gene structure display, and one section of the left side is promotor gene, and one section on the right is structural gene, and promotor gene has the functional structure gene expression immuno-enzymatic quantity that starts the right side and the function of catalytic activity. And structural gene has the immunoenzymatic effect of expression under the promotor gene in illustrated left side starts, as long as its structure is constant, the character of expressed enzyme can not change, but the time of its expression and expression is subject to the control of left side promotor gene.
Consult Fig. 2, Fig. 2 is an embodiment key diagram of latent function gene activation device (2), shown in the figure, the device of present embodiment mainly includes frequency generator (21), amplifier (22), gene activation case (23), wherein, frequency generator (21) is connected with amplifier (22) phase telecommunication, normally the wired mode by transmission line connects, be provided with radial shield (231) in the gene activation case (23), amplifier (22) is linked on the radial shield (231) in the gene activation case (23) by output line, the bacterial classification that preparation is activated is placed in the gene activation case (23), open frequency generator (21) is to desired frequency F, amplifier (22) is that the life electric wave of F is amplified with the frequency of input, export by the power that gives provisioning request, be transferred on the radial shield (231), radial shield (231) activates the latent function gene of the bacterial classification in gene activation case (23) namely by frequency F radiation electric wave.
Frequency generator (21) and amplifier (22) are electronic products commonly used, can directly buy or self manufacture in market, and radial shield (231) is the radio wave transmission antenna, can adopt the structures such as tabular, shaft-like, netted. Consult Fig. 3, Fig. 3 is the another embodiment of latent function gene excitation apparatus (2), the embodiment that adopts wireless laying mode, on Fig. 2 basis, changed, described device includes frequency generator (21), amplifier (22), gene activation case (23), particularly, can also include transmitter unit (24), receiving element (25), wherein, transmitter unit (24) is connected with frequency generator (21), the electric wave that frequency generator (21) occurs is exported away through transmitter unit (24), export to receiving element (25), the way of output can be wireless, also can be wired, receiving element (25) is connected with amplifier (22), receiving element (25) receives the electric wave signal that transmitter unit (24) is launched, when transmitter unit (24) is exported with the radio wave form, be connected without transmission line between receiving element (25) and the transmitter unit (25), as frequency generator (21) and the transmitter unit (24) of radiating portion, can separate with amplifier (22) and gene activation case (23) with the receiving element (25) as receiving unit. This set can bring convenience in some cases. As long as receiving element (23) can be received the signal that obtains emission part branch emission smoothly, be separated by far away also be fine, therefore, the life electric wave that sometimes can adopt the mode of wireless remote control to carry out bacterial classification excites.
The most suitable employing wireless mode of the laying of the embodiment of this figure excites bacterial classification.
Frequency generator among Fig. 2 and Fig. 3 (21) also usually is called electric wave transmitter (21), and transmitter unit (24) is installed in the electric wave transmitter (21) sometimes, and this specification also has employing. Consult Fig. 4, Fig. 4 adopts barms to carry out the method step key diagram of gene regulation. As everyone knows, common yeast is the zymophyte of the many kinds of substances such as class fermentation starch, carbohydrate, protide, multiplex bacterial classification in brewing alcoholic beverages, make bread, make various food, make medicine and multiple product thereof, although saccharomycetic various in style, Various Functions, but never the people utilizes the expressed specific proteins of yeast, activate respectively, regulate B, T, K, NK immunogene function, to carry out recessive gene in the method for not using patent of the present invention be not possess above-mentioned functions before activating to these yeast cells certainly.
The below is used for regulation and control B cellular immune function gene yeasts " latent function gene " as example to activate, and the present invention's method step in this regard is described:
A large amount of gene technology studies have shown that a complete B cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of B cellular immune function gene, and another part is the structural gene of B cellular immune function gene. The structural gene of B cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control B cellular immune function gene of B cellular immune function gene. Therefore, keep the character of the expressed enzyme of B cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of B cellular immune function gene constant; The structural gene that reaches B cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of B cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation B cellular immune function gene protein.
The present invention adopts manual simulation's " life electric wave " to activate the method for yeast " latent function gene ", it is characterized in that, comprises the steps:
1. bacterial classification active device (2) is set,
2. prepare culture medium first (3) according to the composition of subordinate list one, and sterilization treatment:
Subordinate list one: activate regulation and control (B, T, K, NK) cell used yeast " recessiveness
Functional gene " the medium component table
Medium component | Quantity |
Sweet mellow wine | 16g |
K 2HPO 4 | 0.25g |
MgSO 4·7H 2O | 0.2g |
NaCL | 0.22g |
CaSO 4·H 2O | 0.5g |
CaCO 3 | 6.0g |
Urea | 0.2--0.5g |
Serum | 100--300ml |
Aquae destillata | 700--900mL |
3. select suitable yeast specie, selectable yeast sees attached list three, and subordinate list three is listed in this specification last part, optional wherein any one or more, the Candida arborea of genus class 14 or IFFI class saccharomyces cerevisiae etc. under for example selecting are according to active yeast cell/culture medium 〉=1 * 108The ratio of Ge/1000ml is injected among the training Raising Ji Jia (3), and And cultivates the gene activation case (23) that the culture medium first (3) of injecting barms is put into bacterial classification active device (2),
4. the temperature in the maintainer gene maintainer gene activation case (23) was cultivated H1 hour in the T1 scope, and wherein, T1 can be between 37 ± 5 ℃, and H1 can be between 24-56 hour,
5. open the frequency generator (21) of bacterial classification active device (2), to regulation and control B cell, output frequency is adjusted to the F1 scope. The F1 scope can be 6000M to 18000MHz,
6. when bacterial classification active device (2) adopts wireless transmission method shown in Figure 3, the electromagnetic field intensity of the output of transmitter unit (24) is adjusted to the E1 scope, E1 can be 230 ± 15mv/cm,
7. the receive frequency of receiving element (25) is transferred on the frequency consistent with transmitter unit (24) tranmitting frequency, regulate simultaneously the distance L 1 between transmitter unit (24) and the receiving element (25), L1 can be 100 ± 20cm, according to L1 and go out the output voltage V 1 of transmitter unit (24) according to the numerical computations of E1, when L1 is 100cm, output voltage V 1=(230 ± 15mv/cm) * 100cm=21.5V to 24.5V, when bacterial classification active device (2) adopts wired direct way of output shown in Figure 2, output voltage and field intensity are determined with reference to above-mentioned steps, make the field intensity in gene activation case (23) identical
8. under these conditions, and the temperature conditions of maintenance T1, activating H2 hour, H2 can be 42-72 hour,
9. the yeast cells that activates through above condition adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation.
The below illustrates respectively the example that activates regulating cell immunologic function gene, among following each embodiment, all adopt gene activation device (2) to Figure 3 shows that example: example one: to activate the method that is used for regulation and control B cellular immune function gene yeasts " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment,
2. select the IFFI1021 yeast specie, according to the ratio of the IFFI1021 cell/culture medium of living, inject the interior culture medium first of gene activation case (23) of gene activation device (2) shown in the accompanying drawing 3,
3. the temperature T 1 in the maintainer gene activation case (23) is between 37 ± 5 ℃, and cultivating H1 is 24-56 hour,
4. open the electric wave transmitter (21) in the gene activation device (2) shown in the accompanying drawing 3, the output wave frequency be adjusted in the 6000M-18000MHz scope of F1,
5. electric wave transmitter (21) is adjusted to through transmitter unit (24) output electromagnetic field intensity: in VF21.5-24.5V (take distance as 100com as the example) scope,
6. the gene activation case (23) of yeast IFFI1021 nutrient solution will be housed, be installed to the output of receiving unit amplifier (22) according to the pattern shown in the accompanying drawing 3, receive frequency and the tranmitting frequency of transmitter are adjusted in the identical F1 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm
7. under these conditions, and the temperature conditions of maintenance T1, activating H2 is 42-72 hour,
8. IFFI1021 yeast cells after above condition activates adopts the side of vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for modulating T cell immunologic function gene yeast " latent function gene ":
A large amount of gene technology studies have shown that a complete T cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of T cellular immune function gene, and another part is the structural gene of T cellular immune function gene. The structural gene of T cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and the immuno-enzymatic of the promotor gene control T cellular immune function gene of T cellular immune function gene are answered ability, i.e. immunoenzymatic tiring. Therefore, keep the character of the expressed enzyme of T cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of T cellular immune function gene constant; The structural gene that reaches T cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of T cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation T cellular immune function gene protein. Example two: activate the method that is used for modulating T cell immunologic function gene yeast " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment,
2. select the IFFI1212 yeast specie, according to IFFI1212 cell/culture medium 〉=1 * 10 of living8The ratio of Ge/1000ml, the culture medium first of gene activation case (23) shown in the injection accompanying drawing 3,
3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour,
4. open the electric wave transmitter (21) shown in the accompanying drawing 3, will exporting wave frequency, to be adjusted to F2 be in the 7000M-19000MHz scope,
5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in V1=21.5-24.5V (take distance as 100cm as the example) scope,
6. the gene activation case (23) of the blake bottle of yeast IFFI1212 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F2 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm
7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour,
8. IFFI1212 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for regulation and control K cellular immune function gene yeasts " latent function gene ":
A large amount of gene technology studies have shown that a complete K cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of K cellular immune function gene, and another part is the structural gene of K cellular immune function gene. The structural gene of K cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control K cellular immune function gene of K cellular immune function gene. Therefore, keep the character of the expressed enzyme of K cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of K cellular immune function gene constant; The structural gene that reaches K cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of K cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation K cellular immune function gene protein. Example three: activate the method that is used for regulation and control K cellular immune function gene yeasts " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment,
2. select the IFFI1301 yeast specie, according to IFFI1301 cell/culture medium 〉=1 * 10 of living8The ratio of Ge/1000ml, the culture medium first of gene activation case (23) shown in the injection accompanying drawing 3,
3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour,
4. open the electric wave transmitter (21) shown in the accompanying drawing 3, will exporting wave frequency, to be adjusted to F3 be in the 8000M-17000MHz scope,
5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in F1=21.5-24.5V (take distance as 100cm as the example) scope,
6. the gene activation case (23) of the blake bottle of yeast IFFI1301 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F3 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm
7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour,
8. IFFI1301 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation. About activating the method step that is used for regulation and control NK cellular immune function gene yeasts " latent function gene ":
A large amount of gene technology studies have shown that a complete NK cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of NK cellular immune function gene, and another part is the structural gene of NK cellular immune function gene. The structural gene of NK cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control NK cellular immune function gene of NK cellular immune function gene. Therefore, keep the character of the expressed enzyme of NK cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of NK cellular immune function gene constant; The structural gene that reaches NK cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of NK cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life electric wave " method, obtains to have the specific yeast of expression regulation NK cellular immune function gene protein. Example four: activate the method that is used for regulation and control NK cellular immune function gene yeasts " latent function gene " and be exemplified below:
1. according to the one-tenth assignment system culture medium first 1000-2000ml of subordinate list one, and sterilization treatment,
2. select the IFFI1048 yeast specie, according to IFFI1048 cell/culture medium 〉=1 * 10 of living8The ratio of Ge/1000ml, the culture medium first of gene activation case (23) shown in the injection accompanying drawing 3,
3. maintainer gene activates temperature in the case (23) between T1=37 ± 5 ℃, cultivated H1=24-56 hour,
4. open the electric wave transmitter (21) shown in the accompanying drawing 3, will exporting wave frequency, to be adjusted to F4 be in the 8000-16000MHz scope,
5. electric wave transmitter (21) output electromagnetic field intensity is adjusted to: in V1=21.5-24.5V (take distance as 100cm as the example) scope,
6. the gene activation case (23) of the blake bottle of yeast IFFI1048 nutrient solution will be housed, be installed to reception part amplifier (22) output according to the pattern shown in the accompanying drawing 3, receive frequency and tranmitting frequency are adjusted in the identical F4 scope, the distance of regulating simultaneously between transmitter unit (24) and the receiving element (25) is 100cm
7. under these conditions, and the temperature conditions of maintenance T1, activate H2=42-72 hour,
8. IFFI1048 yeast cells after above condition activates adopts the method for vacuum freeze drying to make ampoule or make the pulvis preservation.
But the hundreds of saccharomycete that provided 54 genus in this specification subordinate list three are choice for use all, shows listed microorganism but microbe species involved in the present invention is not limited to this.
The activation of this moment the saccharomycete of the various cellular immune function genes of regulation and control of latent function gene be exactly the product of method of the present invention, namely biological immunomodulator can make the injection injection and use.
The below provides the biological immunomodulator injection effectiveness test example of making as stated above. Experiment for example experiment gives an example one:
A. get 60 of wates rats, be divided into totally 3 groups of A, B, C, every group of 20 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt 180 ascites tumors, big white mouse is respectively organized in inoculation respectively.
C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid8Ge/ml), dosage is 0.6ml/kg; B associative ring phosphamide, dosage are 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day.
D. dissect to detect afterwards the size of ascites tumor in seven days, such as following table:Experiment gives an example two:
A. get 90 of wates rats, be divided into totally 3 groups of A, B, C, every group of 30 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group.
B. adopt the U-14 solid tumor, big white mouse is respectively organized in inoculation respectively.
C. from postvaccinal second day, A organizes that (4 species specificity yeast cells all 〉=1 * 10 to this biologic product liquid8Ge/ml), dosage is 0.6ml/kg; B associative ring phosphamide, dosage are 20ug/kg; C organizes to physiological saline 0.6ml/kg. Once a day.
D. take and dissected afterwards the size that ascites tumor is washed in inspection in 14 days, such as following table:
If adopt orally to gavage or add when feeding in the feed, the processing of the domestication that bacterial classification also must acidproof through specific yeast (anti-low PH).
Illustrated that more than the present invention adopts the method for manual simulation's " life electric wave ", activate respectively yeast difference " latent function gene ", obtain 4 kinds of specific yeasts that are respectively applied to activate, regulate and control B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene function. But this 4 species specificity yeast can't be directly used in the oral various immunogenes of regulation and control after the stomach effect that gavage, and this is because they can't adapt to the environment of human body. As everyone knows, be a very complex environment in the human body, and various envirment factor is all changing work all the time. When this 4 species specific yeast enters the approach of small intestine by oral cavity, stomach, be difficult to ensure its ferment born of the same parents' integrality, more therefore the activity of purpose in the Customers ' Legal Right yeast cells ensures that the yeast cells activity is very crucial. The present invention has adopted the envirment factor domestication of 4 species specificity yeast has been cultivated, and the domestication and culture method step is following to be described.
The environmental suitability domestication of 4 kinds of functional microorganisms of the present invention is by realizing such as the device of Fig. 5 and method shown in Figure 6.
Consult Fig. 5, an embodiment key diagram of the strain domestication device (6) that Fig. 5 adopts when being the specific yeast domestication of acid-resistance. Shown in the figure, described domesticating device comprises frequency generator (21), strain domestication tank (26), wherein, electric wave radial shield (261) is installed in the strain domestication tank (26), and the output of frequency generator (21) is connected on the electric wave radial shield (261) by transmission line. When domestication, the bacterial classification after putting into corresponding culture medium and be activated in strain domestication tank (26) is transferred to the frequency that will tame with frequency generator (21), can carry out the electric wave domestication.
Consult Fig. 6, Fig. 6 processes the specific yeast bacterium that has produced certain cellular immune function gene through activating, the key diagram of the step of taming, bacterial classification after being activated will be tamed, could adapt to the livestock vivo environments such as oral cavity and stomach, the domestication step is (to adopt the 1000ml culture medium as example):
81. domesticating device (6) is set,
82. preparation domestication culture medium second (7),
83. get the barms of preserving after activating among the result of some aforementioned method steps, pour in the strain domestication tank (26), the quantity that adds is decided with set domesticating device scale (2) according to demand, during as an example of the 1000ml culture medium example, add species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of such cell8Ge/ml),
84. the culture medium second (7) of respective amount is poured in the bacterial classification A domestication tank (26), for example inject 1000ml,
85. open frequency generator (21), the output electric wave is adjusted on the specific yeast selectivity frequency F of such cellular immunity gene of regulation and control frequently, namely, when regulating and control the specific yeast of B cellular immunity gene, get frequency F1, when the specific yeast of modulating T cell immunogene, get frequency F2, when regulating and control the specific yeast of K cellular immunity gene, get frequency F3, when regulating and control the specific yeast of NK cellular immunity gene, get frequency F4, F1 wherein, F2, F3, F4 is aforesaid each corresponding frequencies, electric wave is exported electric field regulate by 5-10mv/ml, and the employed received-signal strength of 1000ml culture medium second is the 5-10 volt, by the electric wave radial shield (261) in the strain domestication tank (26) bacterial classification in strain domestication tank (26) is carried out acclimation
86. acclimation temperature is T2, the domestication time is H2 hour, and T2 can get 37 ± 5 ℃, and H2 can get 48 to 96 hours,
87. after the domestication strain separating is kept under 0-4 ℃ the condition for subsequent use. Culture medium second (7) composition such as subordinate list two subordinate lists two of special use in the above-mentioned steps, culture medium second component list (take 1000ml as example)
Medium component | Quantity | Explanation |
Wild Jujube Juice | 300ml | With the clear liquid that dry acid jujube/water=the 1g/5ml ratio is made |
Malus baccata juice | 500ml | With the clear liquid that dried Malus baccata/water=the 1g/5ml ratio is made |
(NH 4) 2SO 4 | 0.25g | |
K 2HPO 4 | 0.2g | |
MgSO 4·7H 2O | 0.22g | |
NaCL | 0.5g | |
CaSO 4·2H 2O | 0.3g | |
CaCO 3 | 3.0g | |
Specific yeast nutrient solution after the activation | Each 20ml | Contain active yeast cell 〉=1 * 108Individual/ml |
The bacterial classification of this moment is cultivated through enlarging, and can be made into oral agents, takes to livestock. The method step of the present invention's 4 species specificity yeast environmental suitabilities domestication is described as follows respectively, and equipment therefor is take Fig. 5 as example: (one). the specific yeast step of domestication regulation and control B cellular immunity gene
1. the method according to subordinate list two configures culture medium second, and sterilization treatment,
2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,
3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control B cell8Ge/ml), inject the strain domestication tank (26) shown in the accompanying drawing 5,
4. open wave generator (21) as shown in Figure 5, and be adjusted on the specific yeast selectivity frequency F1 of regulation and control B cellular immunity gene,
5. the electric wave output electric field of regulating as shown in Figure 5 is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and electric-field intensity constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (2). the specific yeast step of domestication modulating T cell immunogene
1. the method according to subordinate list two configures cultivation second, and sterilization treatment,
2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,
3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of modulating T cell8Ge/ml), inject the strain domestication tank (26) shown in the accompanying drawing 5,
4. open wave generator (21) as shown in Figure 5, and be adjusted on the specific yeast selectivity frequency F2 of modulating T cell immunogene,
5. the electric wave output electric-field intensity of regulating as shown in Figure 5 is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (3). the specific yeast step of domestication regulation and control K cellular immunity gene
1. the method according to subordinate list two configures culture medium second, and sterilization treatment,
2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,
3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control K cell8Ge/ml), inject the strain domestication tank (26) shown in the accompanying drawing 5,
4. open wave generator (21) as shown in Figure 5, and be adjusted on the specific yeast selectivity frequency F3 of regulation and control B cellular immunity gene,
5. the electric wave output electric-field intensity of regulating as shown in Figure 5 is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (4). the specific yeast step of domestication regulation and control NK cellular immunity gene
1. the method according to subordinate list two configures culture medium second, and sterilization treatment.
2. get in the strain domestication tank (26) that culture medium second 1000ml is injected into accompanying drawing 5,
3. get species specificity yeast juice 10ml (yeast juice living cells content 〉=1 * 10 of regulation and control NK cell8Ge/ml), inject the strain domestication tank (26) shown in the accompanying drawing 5,
4. open wave generator (21) as shown in Figure 5, and be adjusted on the specific yeast selectivity frequency F4 of regulation and control B cellular immunity gene,
5. the electric wave output electric-field intensity of regulating as shown in Figure 5 is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v),
6. keep above-mentioned wave frequency and received-signal strength constant, at 37 ± 5 ℃ temperature conditions
Cultivate after 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. Consult Fig. 7, Fig. 7 is with through the bacterial classification of the domestication square frame key diagram to the step of making the oral biological immunomodulator that gavages the agent form.
Specific yeast involved in the present invention is cultivated totally 4 kinds. This 4 species specificity yeast only is to have obtained seed (71) after obtaining through said method, make a large amount of biologic products of the present invention, and the specific yeast of capacity must be arranged, and therefore needs to enlarge and cultivates. The 4 species specificity yeast juices (72) that the present invention obtains through above specific yeast culture process respectively, this yeast juice is alone also can, but mix with then effect is better, therefore, as required, select several for example two kinds, three kinds, four kinds to mix, for example send into the hybrid technique step (73) of 4 primary yeast liquid. 4 species specificity yeast juice mixtures enter concentration step (74), then carry out canned (75), become finished product.
Consult Fig. 8, Fig. 8 is that special yeast enlarges the cultivation schematic diagram after activating, the standby yeast of the spy who adopts enlarges culture process device (8) and includes frequency generator (21) and three culture tank (A, B, C), be provided with electric wave expelling plate (81) in each culture tank, frequency generator (21) is connected with each electric wave expelling plate (81) by output line, after frequency generator (21) is opened, the electric wave of its output through electric wave expelling plate (81) at corresponding culture tank (A, B, C) emission enlarges cultivation to special yeast in. The frequency generator here (21) can be identical with aforesaid frequency generator.
The below describes respectively the cultivation of the specific yeast of the immunogene function of B, T, K, NK cell.
About the specific yeast culture process journey of the specific yeast culture process adjusting involved in the present invention B cellular immune function of regulating B cellular immunity gene function as shown in Figure 8.
Consult Figure of description 8 of the present invention, the specific yeast culture process step of adjusting B cellular immune function involved in the present invention is as follows:
81.1 according to the one-tenth assignment system culture medium the third of subordinate list four, and after sterilization treatment, be injected into respectively in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8,
81.2 will activate through manual simulation's life electric wave method, and the specific yeast of the adjusting B cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then 8A tank seed liquor is injected into enlarge in the culture medium of 8B tank according to certain ratio and cultivates, the ratio of injecting is: 8A seed liquor/8B nutrient solution=5ml/1000ml
81.3 regulate wave generator (21), making it export biological electric wave is F1, and calculates according to the requirement of 0.5-1.0v/L simultaneously, and (quantity such as nutrient solution in the hypothesis 8B tank is 50L to set received-signal strength, single intensity of wave should be: 0.5-1.0v/L * 50L=25v-50v)
81.4 keep in the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ of conditions,
81.5 when the specific yeast living cells of regulating B cellular immunity gene in the B tank reaches 2,000,000,000/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to lower road hybrid technique.
The present invention relates to regulate the specific yeast culture process engineering of T cellular immune function shown in this accompanying drawing 8 about the specific yeast culture process of regulating T cellular immunity gene function. Consult this accompanying drawing 8, the specific yeast culture process step of adjusting T cellular immune function involved in the present invention is as follows:
82.1 according to the one-tenth assignment system culture medium the third of subordinate list four, well is injected into respectively in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8 after sterilization place li,
82.2 will activate through manual simulation's life electric wave method, and the specific yeast of the adjusting T cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then 8A tank seed liquor is injected into enlarge in the cultivation of 8B tank according to certain ratio and cultivates, the ratio of injecting is: 8A seed liquor/8B nutrient solution=5ml/1000ml
82.3 regulate wave generator (21), making it export biological electric wave is F2, and calculates with the requirement according to 0.5-1.0v/L, and (quantity such as nutrient solution in the hypothesis 8B tank is 50L to set received-signal strength, single intensity of wave should be: 0.5-1.0v/L * 50L=25v-50v)
82.4 keep in the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ of conditions,
82.5 when the specific yeast living cells of regulating T cellular immunity gene in the B tank reaches 2,000,000,000/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to lower road hybrid technique.
About the specific yeast culture process engineering of the specific yeast culture process adjusting involved in the present invention K cellular immune function of regulating K cellular immunity gene function shown in this accompanying drawing 8. Consult this accompanying drawing 8, the specific yeast culture process step of adjusting K cellular immune function involved in the present invention is as follows:
83.1 according to the one-tenth assignment system culture medium the third of subordinate list four, well is injected into respectively in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8 after sterilization treatment,
83.2 will activate through manual simulation's life electric wave method, and the specific yeast of the adjusting K cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then be injected into to enlarge in the culture medium of 8B tank according to certain ratio 8A tank seed liquor and cultivate, the ratio of injecting is: 8A seed liquor/B nutrient solution=5ml/1000ml
83.3 regulate wave generator (21), making it export biological electric wave is F3, and calculates according to the requirement of 0.5-1.0v/L simultaneously, and (quantity such as nutrient solution in the hypothesis 8B tank is 50L to set received-signal strength, single intensity of wave should be: 0.5-1.0v/L * 50L-25v-50v)
83.4 keep in the constant situation of above-mentioned wave frequency and intensity of wave, cultivated 56-72 hour under 37 ± 5 ℃ of conditions,
83.5 when the specific yeast living cells of regulating K cellular immunity gene in the 8B tank reaches 2,000,000,000/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to lower road hybrid technique.
About regulating the specific yeast culture process of NK cellular immunity gene function
The specific yeast culture process engineering of adjusting NK cellular immune function involved in the present invention is shown in this accompanying drawing 8. Consult this accompanying drawing 8, the specific yeast culture process step of adjusting NK cellular immune function involved in the present invention is as follows:
84.1 according to the composition of subordinate list four preparation culture medium the third, and after sterilization treatment, be injected into respectively in culture tank 8A, 8B, the 8C tank of this accompanying drawing 8,
84.2 will activate through manual simulation's life electric wave method, and the specific yeast of the adjusting NK cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank 8A shown in this accompanying drawing 8, as seed liquor, then 8A tank seed liquor is injected into enlarge in the culture medium of 8B tank according to certain ratio and cultivates, the ratio of injecting is: 8A seed liquor/B nutrient solution=5ml/1000ml
84.3 regulate wave generator (21), making it export biological electric wave is F4, and calculates according to the requirement of 0.5-1.0v/L simultaneously, and (quantity such as nutrient solution in the hypothesis 8B tank is 50L to set received-signal strength, single intensity of wave should be: 0.5-1.0v/L * 50L=25v-50v)
84.4 keep in the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ of conditions,
84.5 when the specific yeast living cells of regulating NK cellular immunity gene in the 8B tank reaches 2,000,000,000/ml, in 8B tank yeast juice input 8C tank, prepare to be transported to lower road hybrid technique.
Consult Fig. 9, Fig. 9 is specific yeast liquid hybrid technique schematic diagram, the mixing arrangement (9) that adopts includes 4 storage tanks (9A, 9B, 9C, 9D) and a blending tank (M), B, T, K, NK Cell regulate yeast juice have been stored up respectively in 4 storage tanks, usually these all are large tanks, have used literal as decorating and explanation on the tank surface.
4 related species specificity yeast juices of the present invention mix and can according to different targets, adopt different proportion to mix. In this example, be to mix according to the ratio of following table. Specific yeast liquid mixed proportion list (in the 4000L mixed liquor)
The mixing of specific yeast liquid of the present invention is to carry out according to the mode of this accompanying drawing 9. Consult this accompanying drawing 9, this technique realizes by following steps:
Specific yeast liquid kind | Quantity | Ratio | Requirement | |
Regulation and control B cellular immunity gene function | 1000L | 25% | In nutrient solution |
Modulating T cell immunogene function | 1000L | 25% | In nutrient solution | |
Regulation and control K cellular immunity gene function | 1000L | 25% | In nutrient solution | |
Regulation and control NK cellular immunity gene function | 1000L | 25% | In nutrient solution |
91. 4 species specificity yeast juices are input to respectively in storage tank 9A, 9B, 9C, the 9D tank,
92. 4 species specificity yeast juices in storage tank 9A, 9B, 9C, the 9D tank are input among the blending tank M according to the ratio of equivalent mix,
93. mixed yeast juice is injected in the concentration technology shown in the accompanying drawing 10, prepares concentrated.
Consult Figure 10, Figure 10 is specific yeast liquid concentration technique key diagram, and shown in the figure, device therefor is thickener 10A and 10B, has used literal as decoration and explanation on the thickener surface, and has used arrow explanation mixed liquor to flow to.
Concentration technology is with the mixed liquid concentration of 4 species specificity yeast juices in the above-mentioned hybrid technique, and concentrated purpose is that the requirement when reaching the embedding finished product is set. Concentration technology is divided into two-stage, and being transported to thickener 10B after the first order concentrates with thickener 10A, to carry out the second level concentrated again, and finally reaching enrichment is about 64%. Concentrating of different in nature yeast juice involved in the present invention, realize according to following steps:
10.1 will be transported among this concentration technology first order thickener 10A shown in Figure 10 through the mixed mixed liquor of above-mentioned hybrid technique,
10.2 in the tank of first order thickener 10A, the specific yeast mixed liquor is concentrated into 80% (measurement basis calculation), then be transported among the thickener 10B of the second level concentrated, also can adopt the concentrated or method such as concentrated of heating of cold air Vacuum Concentration, normal temperature partial vacuum, but all must be with the active benchmark of retention performance yeast cells without which kind of concentrated mode
10.3 again be concentrated into 80% in the tank of second level thickener 10B, the condition when still concentrating with the first order about concentrated requirement is identical, is transported to immediately dosing technology after concentrating and prepares potting.
Figure 11 is cooling metering and finished product packing schematic diagram, be called for short the dosing technology step, device therefor shown in the figure includes cooler (11A), dosing machine (11B), potting machine (11C), with finished product bottle (11D), used corresponding text decoration on each machine, with arrow flow sequence is described among the figure.
Dosing technology involved in the present invention is cooling, metering and three processes of embedding. The purpose of cooling is that the temperature rise that causes in the concentration process will be got off, in order to avoid produce gas after the embedding; Metering is to concentrate the control requirement that whether reaches total amount in order to check, and prepares for the used bottle quantity of embedding; Embedding is the final step of specific yeast liquid being made the finished product main technique. This technique is to realize according to the step shown in this accompanying drawing 11:
11.1 the special sexupara mixed liquor after will concentrating is transported to the cooling shown in this accompanying drawing 11 but in the tank of machine (11A), is cooled to 12-15 ℃,
11.2 will be through the mixed liquor of cooler tank cooling, be transported in the tank of dosing machine (11B) and measure, the mixed liquor after the metering is transported to filling and sealing machine (11C) carries out embedding, make finished product (11D).
Become oral or gavage the biological immunomodulator of liquid form, be used for giving anti-and cure aftosa.
The microbe species that table 3. this patent is related
(showing listed microorganism but be not limited only to this)
The affiliated class-01 that belongs to | Saccharomyces cerevisiae Hansen saccharomyces cerevisiae | |||
Former purposes-01 | Wine brewing, edible and food manufacturing | |||
ACCC2034 | ACCC2035 | ACCC2036 | ACCC2037 | ACCC2038 |
ACCC2039 | ACCC2040 | ACCC2041 | ACCC2042 | AS2.1 |
AS2.4 | AS2.11 | AS2.14 | AS2.16 | AS2.56 |
AS2.69 | AS2.70 | AS2.93 | AS2.98 | AS2.101 |
AS2.109 | AS2.110 | AS2.112 | AS2.139 | AS2.173 |
AS2.174 | AS2.182 | AS2.196 | AS2.242 | AS2.336 |
AS2.346 | AS2.369 | AS2.374 | AS2.375 | AS2.379 |
AS2.380 | AS2.382 | AS2.390 | AS2.393 | AS2.395 |
AS2.396 | AS2.397 | AS2.398 | AS2.399 | AS2.400 |
AS2.406 | AS2.408 | AS2.409 | AS2.413 | AS2.414 |
AS2.415 | AS2.416 | AS2.422 | AS2.423 | AS2.430 |
AS2.431 | AS2.432 | AS2.451 | AS2.452 | AS2.453 |
AS2.458 | AS2.460 | AS2.463 | AS2.467 | AS2.486 |
AS2.501 | AS2.502 | AS2.503 | AS2.504 | AS2.516 |
AS2.535 | AS2.536 | AS2.558 | AS2.560 | AS2.561 |
AS2.562 | AS2.576 | AS2.593 | AS2.594 | AS2.614 |
AS2.620 | AS2.628 | AS2.631 | AS2.666 | AS2.982 |
AS2.1190 | AS2.1364 | AS2.1396 | IFFI1001 | IFFI1002 |
IFFI1005 | IFFI1006 | IFFI1008 | IFFI1009 | IFFI1010 |
IFFI1012 | IFFI1021 | IFFI1027 | IFFI1037 | IFFI1042 |
IFFI1043 | IFFI1045 | IFFI1048 | IFFI1049 | IFFI1050 |
IFFI1052 | IFFI1059 | IFFI1060 | IFFI1063 | IFFI1202 |
IFFI1203 | IFFI1206 | IFFI1209 | IFFI1210 | IFFI1211 |
IFFI1212 | IFFI1213 | IFFI1214 | IFFI1215 | IFFI1220 |
IFFI1221 | IFFI1224 | IFFI1247 | IFFI1248 | IFFI1251 |
IFFI1270 | IFFI1277 | IFFI1287 | IFFI1289 | IFFI1290 |
IFFI1291 | IFFI1292 | IFFI1293 | IFFI1297 | IFFI12300 |
IFFI1301 | IFFI1302 | IFFI1307 | IFFI1308 | IFFI1309 |
IFFI1310 | IFFI1311 | IFFI1335 | IFFI1336 | IFFI1337 |
IFFI1338 | IFFI1339 | IFFI1340 | IFFI1345 | IFFI1348 |
IFFI1396 | IFFI1397 | IFFI1399 | IFFI1411 | IFFI1413 |
The affiliated class-02 that belongs to | Saccharomyces cerevisiae Hansen Var.ellipsoideus (Hansen) Dekker ellipse brewing yeast | |||
Former purposes-02 | Wine brewing, edible and food manufacturing | |||
ACCC2043 | AS2.2 | AS2.3 | AS2.8 | AS2.53 |
AS2.163 | AS2.168 | AS2.483 | AS2.541 | AS2.559 |
AS2.606 | AS2.607 | AS2.611 | AS2.612 | |
The affiliated class-03 that belongs to | Saccharomyces chevalieri Xue Guilliermond watt yeast | |||
Former purposes-03 | Wine brewing, edible and food manufacturing | |||
AS2.131 | AS2.213 | |||
The affiliated class-04 that belongs to | Saccharomyces delbrueckii Dare cloth yeast | |||
Former purposes-04 | Food fermentation | |||
AS2.285 | ||||
The affiliated class-05 that belongs to | Saccharomyces delbrueckii Lindner ver.mongolicus (Saito) Lodder et van Rij Mongolia Dare cloth yeast | |||
Former purposes-05 | Food fermentation | |||
AS2.209 | AS2.1157 |
The affiliated class-06 that belongs to | Saccharomyces exiguous Hansen saccharomyces exiguus | |||
Former purposes-06 | Food fermentation | |||
AS2.349 | AS2.1158 | |||
The affiliated class-07 that belongs to | Saccharomyces fermentati (Saito) Lodder et van Rij saccharomyces fermentati | |||
Former purposes-07 | Food fermentation | |||
AS2.286 | AS2.343 | |||
The affiliated class-08 that belongs to | Saccharomyces Logos van laer et Denamur ex Jorgensen Lip river lattice yeast | |||
Former purposes-08 | The functions such as class, food fermentation make grape wine | |||
AS2.156 | AS2.327 | AS2.335 | ||
The affiliated class-08 that belongs to | Saccharomyces mellis (Fabian et Quinet) Lodder et kreger van Rij saccharomyces mellis | |||
Former purposes-08 | With carbohydrate, starch based fermentation | |||
AS2.195 | ||||
The affiliated class-09 that belongs to | The little saccharomyces ellipsoideus of Saccharomyces mellis Microellipsoides Osterwalder | |||
Former purposes-09 | With carbohydrate, starch based fermentation | |||
AS2.699 | ||||
The affiliated class-10 that belongs to | Saccharomyces oviformis Osteralder ellipsoideus yeast | |||
Former purposes-10 | With carbohydrate, starch based fermentation | |||
AS2.100 | ||||
The affiliated class-11 that belongs to | Saccharomyces rosei (Guilliermond) Lodder et Kreger van Rij Luo Si yeast |
Former purposes-11 | With carbohydrate, starch based fermentation | |||
AS2.287 | ||||
The affiliated class-12 that belongs to | Saccharomyces rouxii Boutroux Lu Shi yeast | |||
Former purposes-12 | With carbohydrate, starch based fermentation, make edible paste, soy sauce etc. | |||
AS2.178 | AS2.180 | AS2.370 | AS2.371 | |
The affiliated class-13 that belongs to | Saccharomyces Sake Yabe saccharomyces sake | |||
Former purposes-13 | With carbohydrate, starch based fermentation | |||
ACCC2045 | ||||
The affiliated class-14 that belongs to | Candida arborea Candida arborea | |||
Former purposes-14 | Be used for feed, amino acids manufacturing, cellulose, starch, carbohydrate, protein fermentation etc. | |||
AS2.566 | ||||
The affiliated class-15 that belongs to | Candida lambica (Lindner et Genoud) van.Uden et Buckley; Bright Bick Candida | |||
Former purposes-15 | Be used for producing ester under the high temperature, make flavoring essence etc. | |||
AS2.1182 | ||||
The affiliated class-16 that belongs to | Candida Krusei (Castellani) Berkhout Cruise yeast | |||
Former purposes-16 | Be used for wine brewing, make feed, amino acids, albumen etc. | |||
AS2.1045 | ||||
The affiliated class-17 that belongs to | Candida lipolytica (Harrison) Diddens et Lodder Candida lipolytica |
Former purposes-17 | Be used for oil dewaxing, make organic acid substance | |||
AS2.1207 | AS2.1216 | AS2.1220 | AS2.1379 | AS2.1398 |
AS2.1399 | AS2.1400 | |||
The affiliated class-17 that belongs to | The medium-sized level and smooth Candida of Candida parapsilosis (Ashford) Langeron et Talice Var. intermedia Van Rij et Verona | |||
Former purposes-17 | Utilize carbohydrate, starch based fermentation to make feed | |||
AS2.491 | ||||
The affiliated class-18 that belongs to | Candida parapsilosis (Ashford) Langeron et Talice Candida parapsilosis | |||
Former purposes-18 | Utilize pentose class hydrolyzate to make feed | |||
AS2.590 | ||||
The affiliated class-19 that belongs to | Candida pulcherriman (Lindner) Windisch iron oxide red yeast | |||
Former purposes-19 | Stimulating growth | |||
AS2.492 | ||||
The affiliated class-20 that belongs to | Candida rugosa (Anderson) Diddens et Lodder fold candida | |||
Former purposes-20 | Oil dewaxing, production organic acid etc. | |||
AS2.511 | AS2.1367 | AS2.1369 | AS2.1372 | AS2.1373 |
AS2.1377 | AS2.1378 | AS2.1384 | ||
The affiliated class-21 that belongs to | Candida tropicalis (Castellani) Berkhout candida tropicalis | |||
Former purposes-21 | Carbohydrate fermentation; Cellulose, hemicellulose fermentation; Paper pulp already ferments; Sulfurous acid tobacco fermentation; Feed is made; Yeast extract, ergosterol manufacturing etc. | |||
ACCC2004 | ACCC2005 | ACCC2006 | AS2.164 | AS2.402 |
AS2.564 | AS2.565 | AS2.567 | AS2.568 | AS2.617 |
AS2.637 | AS2.1387 | AS2.1397 |
The affiliated class-22 that belongs to | Candida utilis Henneberg Lodder et Kreger Van Rij candida utili | |||
Former purposes-22 | Eat and the feed manufacturing | |||
AS2.120 | AS2.281 | AS2.1180 | ||
The affiliated class-23 that belongs to | The false capsule yeast of Crebrothecium ashbyii (Guilliermond) Routein=Eremothecium ashbyii Guilliermond A Shu | |||
Former purposes-23 | Be used for riboflavin manufacturing etc. | |||
AS2.481 | AS2.482 | AS2.1197 | ||
The affiliated class-24 that belongs to | Geotrichum candidum Link geotrichum candidum | |||
Former purposes-24 | Feed is made; | |||
ACCC2016 | AS2.361 | AS2.498 | AS2.616 | AS2.1035 |
AS2.1062 | AS2.1080 | AS2.1132 | AS2.1175 | AS2.1183 |
The affiliated class-25 that belongs to | Hansenula anomala (Hansen) H et P sydow Hansenula anomala | |||
Former purposes-25 | Spices is made; Improve drinks fragrance, food flavor etc.; | |||
ACCC201 | AS2.294 | AS2.295 | AS2.296 | AS2.297 |
AS2.298 | AS2.299 | AS2.300 | AS2.302 | AS2.338 |
AS2.339 | AS2.340 | AS2.341 | AS2.470 | AS2.592 |
AS2.641 | AS2.642 | AS2.782 | AS2.635 | AS2.794 |
The affiliated class-26 that belongs to | Hansenula arabitolenes Fang arabite Hansenula yeast | |||
Former purposes-26 | Produce the pure and mild glycerine of arabinose; | |||
AS2.887 | ||||
The affiliated class-27 that belongs to | The outstanding fourth Hansenula yeast of Hansenula jadinii (A.et R Sartory Weill et Meyer) Wickerham |
Former purposes-27 | Do not find the report of application | |||
ACCC2019 | ||||
The affiliated class-28 that belongs to | Hansenula saturnus (Klocker) H et P sydow Saturn Hansenula yeast | |||
Former purposes-28 | Do not find the report of application | |||
ACCC2020 | ||||
The affiliated class-29 that belongs to | Hansenula schneggii (Weber) Dekker Amur Hansenula yeast | |||
Former purposes-29 | Do not find the report of application | |||
AS2.304 | ||||
The affiliated class-30 that belongs to | The inferior film Hansenula yeast of Hansenula subpelliculosa Bedford | |||
Former purposes-30 | From multiple liquor raw material processed or poor slag, obtain, but do not find the report of application | |||
AS2.740 | AS2.760 | AS2.761 | AS2.770 | AS2.783 |
AS2.790 | AS2.798 | AS2.866 | ||
The affiliated class-31 that belongs to | Kloeckera apiculata (Reess emend.Klocker) Janke lemon shape Ke Leke yeast | |||
Former purposes-31 | Do not find the report of application | |||
ACCC2022 | ACCC2023 | AS2.197 | AS2.496 | AS2.714 |
ACCC2021 | AS2.711 | |||
The affiliated class-32 that belongs to | Lipomycess starkeyi Lodder et van Rij saccharomyces oleaginosus | |||
Former purposes-32 | Do not find the report of application | |||
AS2.1390 | ACCC2024 | |||
The affiliated class-33 that belongs to | Pichia farinose (Lindner) Hansen pichia farinose | |||
Former purposes-33 | Do not find the report of application | |||
ACCC2025 | ACCC2026 | AS2.86 | AS2.87 | AS2.705 |
AS2.803 | ||||
The affiliated class-34 that belongs to | Pichia membranaefaciens Hansen Pichia membranaefaciens | |||
Former purposes-34 | Do not find the report of application | |||
ACCC2027 | AS2.89 | AS2.661 | AS2.1039 | |
The affiliated class-35 that belongs to | Rhodosporidium toruloides Banno spore yeast of red winter | |||
Former purposes-35 | Do not find the report of application | |||
ACCC2028 | ||||
The affiliated class-35 that belongs to | Rhodotorula glutinis (Fresenius) Harrison rhodotorula | |||
Former purposes-35 | Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc. | |||
AS2.2029 | AS2.280 | ACCC2030 | AS2.102 | AS2.107 |
AS2.278 | AS2.499 | AS2.694 | AS2.703 | AS2.704 |
AS2.1146 | ||||
The affiliated class-36 that belongs to | The little rhodotorula of Rhodotorula minuta (Saito) Harrison | |||
Former purposes-36 | Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc. | |||
AS2.277 | ||||
The affiliated class-37 that belongs to | Rhodotorula rubar (Demme) Lodder rhodothece rubra | |||
Former purposes-37 | Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment, feed etc. | |||
AS2.21 | AS2.22 | AS2.103 | AS2.105 | AS2.108 |
AS2.140 | AS2.166 | AS2.167 | AS2.272 | AS2.279 |
AS2.282 | ACCC2031 |
The affiliated class-38 that belongs to | Saccharomyces carlsbergensis Hansen Ka Ersi yeast | |||
Former purposes-38 | Make food, brew alcoholic beverages, feed etc. | |||
AS2.113 | ACCC2032 | ACCC2033 | AS2.312 | AS2.116 |
AS2.118 | AS2.121 | AS2.132 | AS2.162 | AS2.189 |
AS2.200 | AS2.216 | AS2.265 | AS2.377 | AS2.417 |
AS2.420 | AS2.440 | AS2.441 | AS2.443 | AS2.444 |
AS2.459 | AS2.595 | AS2.605 | AS2.638 | AS2.742 |
AS2.745 | AS2.748 | AS2.1042 | ||
The affiliated class-39 that belongs to | Saccharomyces uvarum Beijer saccharomyces uvarum | |||
Former purposes-39 | Make food, brew alcoholic beverages, feed etc. | |||
IFFI1023 | IFFI1032 | IFFI1036 | IFFI1044 | IFFI1072 |
IFFI1205 | IFFI1207 | |||
The affiliated class-40 that belongs to | Saccharomyces willianus Saccardo Weir yeast | |||
Former purposes-40 | Make food, brew alcoholic beverages, feed etc. | |||
AS2.5 | AS2.7 | AS2.119 | AS2.152 | AS2.293 |
AS2.381 | AS2.392 | AS2.434 | AS2.614 | AS2.1189 |
The affiliated class-41 that belongs to | Saccharomyces sp. saccharomycete | |||
Former purposes-41 | Make brandy etc. | |||
AS2.311 | ||||
The affiliated class-42 that belongs to | Saccharomycodes ludwigii Hansen rood class yeast | |||
Former purposes-42 | Do not see the report of application | |||
ACCC2044 | AS2.243 | AS2.508 |
The affiliated class-43 that belongs to | Saccharomycodes sinenses Yue China class yeast | |||
Former purposes-43 | Do not see the report of application | |||
AS2.1395 | ||||
The affiliated class-44 that belongs to | Schizosaccharomyces octosporus Beijerinck eight spore fission yeasts | |||
Former purposes-44 | Do not see the report of application | |||
ACCC2046 | AS2.1148 | |||
The affiliated class-45 that belongs to | Schizosaccharomyces pombe Lindner chestnut wine fission yeast | |||
Former purposes-45 | Lactose fermenters, brew alcoholic beverages, feed etc. | |||
ACCC2047 | ACCC2048 | AS2.214 | AS2.248 | AS2.249 |
AS2.255 | AS2.257 | AS2.259 | AS2.260 | AS2.274 |
AS2.994 | AS2.1043 | AS2.1149 | AS2.1178 | IFFI1056 |
The affiliated class-46 that belongs to | Sporobolomyces roseus Kluyver et van Niel shadow yeast | |||
Former purposes-46 | Lactose fermenters, brew alcoholic beverages, feed, antibiotic etc. | |||
ACCC2049 | ACCC2050 | AS2.19 | AS2.962 | AS2.1036 |
ACCC2051 | AS2.261 | AS2.262 | ||
The affiliated class-47 that belongs to | Torulopsis Candida (Saito) Lodder Torulopsis candida | |||
Former purposes-47 | ||||
AS2.270 | ACCC2052 | |||
The affiliated class-48 that belongs to | The unknown torulopsis of Torulopsis famta (Harrisn) Lodder et van Rij | |||
Former purposes-48 | ||||
ACCC2053 | AS2.685 |
The affiliated class-49 that belongs to | Torulopsis globosa (Olson et Hammer) Lodder et van Rij torulopsis | |||
Former purposes-49 | ||||
ACCC2054 | AS2.202 | |||
The affiliated class-50 that belongs to | The usual torulopsis of Torulopsis inconspicua Lodder et Kreger van Rij | |||
Former purposes-50 | ||||
AS2.75 | ||||
The affiliated class-51 that belongs to | Trichosporon behrendii Lodder et Kreger van Rij shellfish thunder trichosporon cutaneum | |||
Former purposes-51 | ||||
ACCC2056 | AS2.1193 | |||
The affiliated class-52 that belongs to | Trichosporon capitatum Diddens et Lodder head trichosporon cutaneum | |||
Former purposes-52 | ||||
ACCC2056 | AS2.1385 | |||
The affiliated class-53 that belongs to | Trichosporon cutaneum (de Beurm et al.) Ota trichosporon cutaneum | |||
Former purposes-53 | ||||
ACCC2057 | AS2.25 | AS2.570 | AS2.571 | AS2.1374 |
The affiliated class-54 that belongs to | Wickerhamia fluorescens (Soneda) Soneda Brunswick yeast | |||
Former purposes-54 | ||||
ACCC2058 | AS2.1388 | |||
Table 4. specific yeast enlarges medium component table (in the 1000L nutrient solution)
Annotate: 1. in the table various liquid all be according to: the ratio of material/water=1/10 is processed into.
Medium component | Quantity |
Haw liquid | 200L |
Fruit of Chinese magnoliavine liquid | 200L |
Date liquid | 200L |
Soybean juice | 200L |
Apple liquid | 200L |
2. upper table nutrient solution will be adjusted in pH2.5 ± 0.2 scope.
Claims (12)
1. one kind is made into livestock with specific immunologic function with the manufacture method of immunomodulator with saccharomycete, for giving anti-and curing various aftosas, it is characterized in that, adopt device and the production method of micro-alternating bio-electric field, adopt the characteristic radio ripple to activate the step of the latent function gene of every Yeasts, include:
1. gene activation device (2) is set,
2. according to one-tenth assignment system culture medium first (3) P of subordinate list one, and sterilization treatment,
3. select the saccharomyces cerevisiae of IFFI1021 yeast specie, according to IFFI1021 wine brewing cell/culture medium 〉=1 * 10 of living8Individual ratio is injected the culture medium first, will cultivate the gene activation case (23) that first (3) is put into gene activation device (2) again,
4. the temperature in the maintenance container was cultivated H1 hour in the T1 scope,
5. open electromagnetic wave transmitter (21), the output wave frequency be adjusted to give in the fixed F scope,
6. electric wave transmitter output electromagnetic field intensity is adjusted in the V1 scope,
7. the gene activation case (23) of the blake bottle of yeast IFFI1021 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the gene activation device (2), receive frequency is adjusted in the identical F scope with the tranmitting frequency of transmitter (24). The distance of regulating simultaneously between transmitter (24) and the receiving instrument (25) is L1,
8. under these conditions, and the temperature conditions of maintenance T1, activate H2 hour,
9. IFFI1021 yeast cells after above condition activates adopts vacuum refrigeration to make ampoule or make the pulvis preservation in dry method.
2. immunomodulator that livestock is used is used for giving anti-and cures various aftosas, it is characterized in that, is the injection agent that utilizes saccharomycete to adopt method described above to make, and orally gavages agent.
3. livestock as claimed in claim 1 is characterized in that with the manufacture method of aftosa immunomodulator, adopts 4 kinds of different characteristic radio magnetic wave frequency F1, F2, F3, F4 can form and activate respectively human body B, T, K, 4 species specificity yeast of the albumen of the latent immunity gene of NK.
4. domestic animal as claimed in claim 1 is held the manufacture method with the aftosa immunomodulator, it is characterized in that, when characteristic frequency F1 is the 6000M-18000MHz scope, activate and be used for regulation and control B cellular immune function because of yeast, when characteristic frequency F2 is the 7000-19000MHz scope, activate and be used for the modulating T cell immunologic function because of yeast, when characteristic frequency F3 is the 8000M-17000MHz scope, activate and be used for regulation and control K cellular immune function because of yeast, when characteristic frequency F4 is the 8000M-16000MHz scope, activate for regulation and control NK cellular immune function because of yeast.
5. livestock as claimed in claim 1 is with the manufacture method of aftosa immunomodulator, it is characterized in that, it is 37 ± 5 ℃ that container when adopting the characteristic radio ripple to activate saccharomycetic latent function gene keeps temperature T 1 scope, incubation time H1 scope when adopting the characteristic radio ripple to activate saccharomycetic latent function gene is 24-56 hour, electric wave transmitter output electromagnetic field unit strength scope is 230 ± 15Mv/cm, distance L 1 between electric wave transmitter and the receiving instrument is 100 ± 10cm, electric wave transmitter output electromagnetic field intensity V1 scope is 21.5 to 24.5 volts, saccharomycetic activationary time H2 is 42 to 72 hours during radiation when adopting the characteristic radio ripple to activate saccharomycetic latent function gene, after adopting the characteristic radio ripple to activate saccharomycetic latent function gene, adopt the mode of vacuum freeze drying to make ampulla or make pulvis and preserve, the quantity P of the culture medium first (3) of employing can be 1000ml or more than.
6. a livestock is with the oral agent manufacture method that gavages of aftosa immunomodulator, it is characterized in that, adopt following steps, first step, adopt the method for claim 1 to produce and activate the barms that is used for regulation and control immunologic function gene, second step, the saccharomycete that has activated the latent function gene is carried out the envirment factor domestication to be cultivated, third step, to cultivate through enlarging through the saccharomycete of envirment factor domestication, decide kind and mix concentratedly by giving, desired saccharomycete human immunity conditioning agent is namely made in modulation.
7. livestock as claimed in claim 6 is characterized in that with the oral manufacture method that gavages agent of aftosa immunomodulator, and the saccharomycete that has activated the latent function gene is carried out the step that the envirment factor domestication is cultivated, and includes:
1. domesticating device (6) is set,
2. according to the good culture medium second of recipe configuration (7) of subordinate list two, and sterilization treatment,
3. get the bacterial classification that culture medium second 1000ml is injected in the domesticating device (6) and tame in the tank (26),
4. get a kind of specific yeast liquid 10ml (yeast juice living cells content 〉=1 * 10 of certain cell of regulation and control at every turn8It is individual/as ml), to inject the bacterial classification of domesticating device (6) and tame tank (26),
5. open the wave generator (21) of domesticating device (6), and be adjusted on the specific yeast selectivity frequency F of this kind of regulation and control cellular immunity gene,
6. the electric wave output voltage of regulating domesticating device (6) is that the employed received-signal strength of every 100ml culture medium is 5-10v,
7. keep above-mentioned wave frequency and intensity of wave constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use.
8. livestock as claimed in claim 6 is characterized in that with the oral manufacture method that gavages agent of aftosa immunomodulator, will comprise following content through enlarging to cultivate to the step of making finished product through the saccharomycete of envirment factor domestication:
1. specific yeast is set enlarges culture apparatus (8),
2. prepare culture medium the third, and after sterilization treatment, be injected into respectively in culture tank 8A, 8B, the 8C tank,
3. will activate through manual simulation's life electric wave method, and the adjusting B that obtains of the cultivation through tolerating low PH (less than PH2.5), T, K, the specific yeast of NK cellular immune function, at every turn a kind of, be input among the culture tank A as seeding tank of specific yeast expansion culture apparatus (8), as seed liquor, then cultivate according to enlarging in the culture medium that gives certainty ratio culture tank A tank seed liquor is injected into culture tank B tank
4. regulate wave generator (21), making it export biological electric wave is F, and the requirement calculating that rises according to 0.5-1.0v/ simultaneously, sets received-signal strength,
5. keep in the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ the temperature conditions,
6. when the specific yeast living cells of regulating the cellular immunity gene in the culture tank B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique.
And selected needed saccharomycetic kind, and various saccharomycetic mixing ratio are mixed as follows, and be concentrated, makes finished product, encapsulation,
1. set up mixing arrangement (9), 4 species specificity yeast juices are input to respectively each storage tank 9A, 9B, 9C, among the 9D,
2. this species specificity yeast juice in each tank is input among the blending tank M according to the ratio of equivalent and mixes,
3. mixed yeast juice is injected in the concentrator (10), prepares to concentrate,
In concentrated (10A) tank of the first order of concentrator (10) with the specific yeast mixed liquor, be concentrated into 80% (measurement basis calculations), then be transported in the third level thickener (10B) and concentrate. Concentrated can adopt that freezing vacuum is concentrated, the normal temperature partial vacuum is concentrated or the method such as concentrated of heating, but which kind of concentrated mode all must be take the work that keeps special yeast cells as benchmark,
5. in second level thickener tank (10B), again be concentrated into 80%,
6. the specific yeast mixed liquor after will concentrating is transported in the cooling tank of cooling packaging system, is cooled to 12-15 ℃,
7. will be transported in the measuring tank and measure through the mixed liquor of cooling tank cooling, the mixed liquor after the metering is transported to embedding in the potting machine, make the oral agent finished product that gavages.
9. such as claim 1 or 6 described livestock immunomodulator manufacture methods, it is characterized in that the barms that is suitable for is any bacterial classification in the table 3.
10. a livestock gavages agent with immunomodulator oral, is used for giving anti-and cures aftosa, it is characterized in that it is manufactured by step claimed in claim 6.
11. the oral agent that gavages as claimed in claim 10 is characterized in that, the saccharomycete of its utilization can be the arbitrarily saccharomycete in the table 3.
12. such as claim 1 or 10 described livestock aftosa immunomodulators, it is characterized in that, it can contain in the following specific yeast bacterium any one, any two kinds, any three kinds, any four kinds, namely regulate the specific yeast bacterium of B cellular immunity gene, namely regulate the specific yeast bacterium of T cellular immunity gene, namely regulate the specific yeast bacterium of K cellular immunity gene, namely regulate the specific yeast bacterium of NK cellular immunity gene.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102068470A (en) * | 2010-12-30 | 2011-05-25 | 香港生命信息康复院有限公司 | Anticancer oral liquid preparation and main component preparation method thereof |
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2001
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102068470A (en) * | 2010-12-30 | 2011-05-25 | 香港生命信息康复院有限公司 | Anticancer oral liquid preparation and main component preparation method thereof |
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