CN1366038A

CN1366038A - Bioregulator for reconfiguring human immune function and its preparing process

Info

Publication number: CN1366038A
Application number: CN 01100584
Authority: CN
Inventors: 张令玉
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-01-16
Filing date: 2001-01-16
Publication date: 2002-08-28

Abstract

A biotic regulator for rebuilding human immunological function features that an electronic information frequency simulating life wave method is used to activate the recessive gene of ordinary yeast to make the said ordinary yeast become specific one, and the characteristic protein (enzyme) expressed by the said specific yeast can activate, regulate and correct the immune gene of B cell, T cell and K cell and the mutation gene of NK cell respectively. The said specific yeast can be used to prepare biological preparations to regulate immune gene for rebuilding immunological function to prevent and cure several diseases such as tumor, diabetes, etc. with high curative effect and no toxic by-effect.

Description

A kind of biological regulator and method for making thereof of rebuilding immune function of human body

One, the technical field of invention

The field that the present invention relates to mainly is a kind of biotechnology research method of novel concept, and the biological regulator with reconstruction immune function of human body that adopts this method to work out. This immunomodulator is used for the adjusting of ill human body, weak body immune function to be rebuild, and the immunologic function of destruction is restored, thus the purpose that reaches disease-resistant, cures the disease. Biotechnology is the heat subject of world today's scientific and technical research. Many scientists, economist and politician think that 21 century is the century of the leading development of biotechnology, are the revolutionary technology of industrialization. Therefore many countries particularly developed country has all dropped into a large amount of manpowers, financial resources are captured biotechnology, attempt breaks through the biotechnology research high point as early as possible, opens the secret of life, thereby creates effective treatment and defend the biologic product of human difficult and complicated illness. Make a general survey of the research of world's biotechnology, major subjects is to concentrate on how to prevent and treat human difficult and complicated illness aspect. From the portion report of American National Science committee in 1998, can recognize that U.S.'s biotechnology research funds wherein have 92% to spend in the medical research. Analyze the history of world's biotechnology research as can be known, numerous research concentrates on the structure of the sequencing of human gene, albumen etc., attempts to find the pathogenesis of human various diseases. This research has spent a large amount of time, has expended countless funds, but the result who obtains is unsatisfactory, does not also find so far accurately pathogenesis of human multiple difficult and complicated cases and cancer. Various gene orders have obtained mensuration, comprise the engineering that human genome is huge like this, also finish in declaration order-checking in 2000, but the product that can be applied in so far in the human difficult and complicated illness treatment are very rare. Although a gene has been recognized its sequence, the mechanism of gene expression enzyme mechanism, substrate for enzymatic activity, and enzymatic activity still is the difficult problem in current biotechnology research field, limiting greatly the development of biotechnology.

As mentioned above, why is biotechnology research in this unfavorable situation? it is considered herein that key is the problem of a research method. What conventional biotechnology research adopted is the methods such as biochemistry, biomolecular science, and these methods all are to be come by the chemical method development of routine. Chemical method is to study the variations such as the chemical combination of non-living matter, decomposition, redox basically. Biology then is lived material, and the per minute per second was all ceaselessly changing when these living matters were per, and older generation's demutation of new generation of dying is alternately constant. Form in organism least unit-cell at one, the carrying out that the metabolism of various materials, the conversion of material etc. are orderly, never can stop. Current biotechnology research is opened this tangible material of the human body as life and all is actually very incomplete. Research of the present invention is thought, the most basic should being formed by the two large divisions of lived organism, and a part is visible, the tangible human body, i.e. tangible material; Another part be conventional theory think cannot see, impalpable invisible material-" soul ". Here " soul " of indication is not the sort of feudalistic superstition said " ghost ", but is present in " life ripple " on each organism. This life ripple is ceaselessly being launched according to fixing frequency and field intensity as same radio transmission ripple, the essential place of Here it is life. On the life entity of a work, this life ripple is ceaselessly launched the existence that could mark life, and after this life ripple lost, life had also just stopped immediately, and what retain is tangible human body material. Do not have the variation of material constituent and weight at this dead human body material of observing in theory of conservation of matter and the human body material that lives, but do not had life. It is considered herein that extremely large essential change has occured the human body with dead these two kinds of forms alive, this metallic substance of life " life ripple " has lost. Making a general survey of all biotechnology research of the world today all is the composition of only studying the human body, the composition of cell, the composition of gene, the composition of albumen etc., and this research seems rather advanced, careful etc., and these researchs are mostly carried out at molecular level, the molecular biology level research that namely Most is fashionable, but lost the research of basic material of life ripple. The human body is a kind of dead material in a sense, and " life ripple " is only material alive, and the human body is the host of " life ripple " just, i.e. emission source. Only have when the human body and " life ripple " organically combine and just can be referred to as the material-biology of life. In the research of life science, the research of " life ripple " is more even more important than the research of the human body in sum! Certainly the research of " life ripple " must just can be a complete biological study in conjunction with the human body.

Why the present invention can successfully obtain the immunomodulator biological products for the various diseases control, and key is to adopt and the diverse research method of conventional biotechnology research. The life rule of living matter-biology has been followed in research involved in the present invention, has both studied the human body characteristic of life entity, studies again life entity active material " life ripple ". The present invention's research " life ripple " can live with sensual necessary condition, and research " life ripple " is to commander and the regulation and control of composition host's tissue, cell, gene, albumen and various active material metabolism simultaneously. By the successful acquisition of this research the biological immunomodulator initiated of the world. Experiment shows, this immunomodulator has safely, has no side effect, effect is remarkable. To those assorted diseases of strange difficulty of causing owing to trouble kinds of tumors, renal failure, uremia, hepatitis B, diabetes, dysfunction, various pathogenic microorganism, virus etc., can be by the regulation and control of this biologic product to immunogene, body immune function is rebuild, reached the purpose of preventing and treating diseases.

The characteristics of biologic product of the present invention are mainly manifested in following on some:

Show that by a large amount of experiments biologic product of the present invention has following characteristic:

1. simulation " life ripple " method, research has the undesired expression immunogene of regulation and control and activates the weak immunogene of ability to express, and proofreaies and correct the characteristic such as immunogene responsibility, is key of the present invention;

2. the biologic product that works out according to the life wave property has significantly specific aim and remarkable result to immunogene activation, regulation and control and the correction of immune B, T, K and NK cell;

3. because this life active compound has emission life wave energy, the function that therefore can regulate and control fast, activate and proofread and correct the human body immunogene;

4. biologic product of the present invention makes body immunity obtain fast rebuilding, the disease that causes to reach control kinds cancer, uremia, hepatitis, diabetes, multiple pathogen and virus by quick regulation and control, activation and correction to immunogene.

5. biologic product of the present invention is not Chinese medicine, neither Western medicine, and more or not antibiotics, interferons, steroids etc., have very high security. Two, the technical background of invention

It is the epoch of chemical technology high development over nearly 200 years, particularly after 20th century 70 years, chemistry, biochemistry, bioengineering and molecular biology etc. have had the development of advancing by leaps and bounds, thereby createing several hundred million ten thousand chemical products, instrument, the clothes of dress, edible food, the house of inhabitation and the cosmetics of use etc. that use from the mankind all are chemical products; Chemicals makes traditional agricultural become chemical farming, makes traditional industry become chemical industry, and traditional grocery trade has become chemical food etc., can say that chemistry is ubiquitous just as microorganism. On agricultural, no matter be the fertilizer that uses, or the agricultural chemicals of diseases prevention, desinsection, the herbicide of weeding all is chemical substance, and in order to obtain the high yield of agricultural product, uses chemical hormone etc. to stimulate the crop Fast Growth. Medically, chemicals has occupied absolute market, particularly occupies 100% market in western countries, has also captured nearly 82% market in China. The various goods such as a large amount of antibiotic, hormone, interferon are ubiquitous. The world of today is the tinkling of jades chemicals that meets the eye on every side everywhere. Many scientists think in recent years, and chemical industry is the human development that brings height, but has also brought healthy harm to the mankind simultaneously. This is because a large amount of chemicals has caused the pollution of environment, no matter is human edible food, and the water of still drinking all is subject to the pollution of chemicals to some extent; A large amount of chemicalses, particularly antibiotics, interferons, hormone medicine endanger larger; Even never use antibiotic, even the people who never takes medicine also can't escape from agricultural product, meat, egg, milk and their goods, and the chemical substance of bringing is to the harm of health. The mankind have absorbed a large amount of chemical substances, have caused queer difficult and complicated illness to cure. This is because various chemical substances have caused human immune system's damage, reduction even disappearance. A large amount of various chemical substances are to the pollution of environment in addition, various pathogenic microorganisms have been brought out, from in recent years in the world various reports as can be known, current pathogenic microorganism or all is greatly improved from pathogenecity no matter on kind. Therefore caused human diseases more and more, more and more stranger, more and more refractory is treated. Very general such as various tumours, hepatitis, diabetes, uremia etc., effectively cure way but go back even to this day neither one. Making a general survey of the various research institutions in the world is found everywhere, a large amount of scientists are engaged in medical research, national governments have dropped into the research of countless financial support medical science, various chemicalses are introduced to the market, yet not only traditional disease is not effectively controlled, and has newly produced on the contrary the assorted disease of various strange difficulties and has ceaselessly challenged to medical circle! In succession put into effect such as AIDS, rabid ox disease etc., medical circle is felt simply helpless!

In sum, medical research direction and the method present according to the world develop down, not only traditional disease can not get effectively preventing and treating, also can bring out on the contrary more, upgrade, more refractory disease, therefore how finding the way of effective, a safe control disease is the too impatient to wait major issue in the world today.

Have data to show, the antibiotic that uses in the world today is kind more than 100 nearly, and hormone also has tens kinds, and various chemical substances are countless especially. These antibiotic, hormone not only use at human body, and the use on the industries such as livestock cultivation, poultry farming, aquaculture is more extensive, and become requisite composition in the feed, be that kind or quantity are all considerably beyond the use amount on the person. Therefore residual a large amount of antibiotic, hormone and number of chemical medicine in human edible meat, egg, milk and the goods are being tortured human human body indirectly, have not only caused the decline of body immunity, and have strengthened the resistance to the action of a drug of human body. As everyone knows, the fifties even the seventies, the mankind suffer from a disease, and only inject 1-2 antibiotic and will cure, and can use the antibiotic of full goods shelves rather to prove effective to today, even serious all the more. It is very effective to look back the epoch that antibiotic just has been born, and a kind of antibiotic just is born, and extensively is applied in medical circle very soon. Along with the mankind to this antibiotic use, find that not only this antibiotic is no longer so efficacious, new disease, the pathogenic microorganism of the heart have been brought out on the contrary, so the mankind have just begun again new antibiotic research and application, when this new antibiotic was so inefficacious again, the antibiotic research and development of renewal had begun. Antibiotic research and development are exactly to have formed so far huge antibiotic series according to this rule development, and human various difficult and complicated cases have also just obtained the opportunity that spreads thereupon simultaneously. This pernicious circulation law has caused the concern of many countries, the country that has becomes very prudent to antibiotic use, it is to be difficult to avoid that yet food and water bring human antibiotic, and the way of cause many people to take to drink mineral water, eating this passiveness of organic food is removed the harm of chemical substance.

Above-mentioned this situation proves absolutely that the human immunity function has had largely injury, and puzzlement how to remove fast and effectively this situation is the task of top priority. Three, the purpose of invention

The objective of the invention is in order to address the above problem. The present invention adopts " life ripple " to activate yeast " latent function gene ", these yeast is become have the specific yeast that produces the human activin immunogene, regulates and control the factors such as immunogene and correction immunogene responsibility. The domestication of passing through again under the present invention's " life ripple " condition is cultivated, then make the biologic product of efficient balance the body immunologic function, and these biologic products are applied to human immunity regulate, reach and regulate the human body Fast Reconstruction that immunologic function descends, damages or lack, thereby reach the purpose of preventing and treating diseases. Four, summary of the invention

Various diseases are the earth of human health, also are to cause human dead main factor. A large amount of studies show that, is which type of disease all is that the body immunity deficiency causes, and various diseases can not occur when the immunity of human body is stronger. But along with the increase at age and wearing out of human body, particularly various chemical substances, various antibiotic, hormone etc. cause the damage of body immunity cell, the immunocyte metabolic disorder, immunogene can not be expressed normally, causes the decline of immunologic function. The human body of immunity decline is easy to be subject to the invasion and attack of various pathogen, also can be subject to the harm of various noxious material, thereby causes various diseases to occur. The decline of body immunity is to be difficult to find according to the detection method of routine. Because detecting human body, conventional method can find that B, T, the immunocyte quantity such as K, NK in the human body are normal. Research of the present invention thinks whether the quantity of immunocyte can only be one of body immune function index normally, but and represent that immunogene expresses normally, can not indicate that more immunoenzymatic character, activity also are normal. What of B, T, K, NK cell quantity are the immunologic function of immunocyte should not be in other words, to recognize also whether the expressed immunoenzymatic character of immunogene in these immunocytes is normal, whether immunoenzymatic catalytic activity is enough, and whether the immunogene responsibility is timely etc. It is considered herein that the expressed immuno-enzymatic of immunogene not only quantity is wanted foot, and enough catalytic effects must be arranged, that is to say that as human body B, T, K, when the NK cell quantity is normal, expressed immuno-enzymatic quantity might not capacity; When immuno-enzymatic quantity is enough, also good catalytic activity might not be arranged; When immuno-enzymatic quantity and activity are normal, can not indicate that immunogene is out of question to the injurious factor responsibility. Immunogene is the same with other genes, is divided into two large divisions's (seeing accompanying drawing one) according to its effect, and left part is the promoter part of immunogene. The promoter of immunogene partly comprises promotor gene, suppressor and regulatory gene. Promotor gene is being undertaken the functions such as time that start the immunoenzymatic quantity of right side functional structure gene expression, expression. The right side is the functional structure Gene Partial of immunogene, in this functional structure Gene Partial, mainly is structural gene. Structural gene has determined the structure of the expressed enzyme of this gene, it is enzymatic property, that is to say that its expressed enzymatic structure and character just can not change as long as the coiled structure of the alkali basic sequence of structural gene and gene strand is constant, but its expressed quantity and expression time might not be correct. Whether timely etc. such as the responsibility of gene pairs substrate, response time. Also need in addition the variation of the time graph of the expressed enzyme of gene and substrate corresponding, that is to say when substrate occurs, when gene makes the control that the expression of enzyme etc. is subjected to the left side promotor gene fully. When the promoter gene of the immunogene of B, T, K, NK immunocyte partly is subject to the impact of various injurious factors, when causing abnormal expression, directly have influence on the expressed immuno-enzymatic quantity of functional structure gene, expression time curve etc. Disturb when the functional structure Gene Partial of immunogene is subject to extrinsic factor, when causing its alkali base or gene strand curly form to change, can affect the expressed immunoenzymatic character of functional structure gene or activity and responsibility etc. In case injurious factor has affected the responsibility of immunogene expressed enzymatic activity, gene expression etc., the immunocompetence of body has substantially been suffered destruction, in the detection of routine and be not easy to find. In addition when the expressed immuno-enzymatic quantity of immunogene, when character is constant, immunogene affects injurious factor, whether make time of expression response consistent also is very important, Here it is said immunogene responsibility, responsibility decline when immunogene, or response time in advance or the decline that all can show immunity when lagging behind, and causes human body ill. Does why the immunogene of B, the T of human body, K, NK cell have the problems referred to above? many endotoxin materials that produce with anion or cationic " free radical ", various pathogenic microorganism, virus etc. are found in research of the present invention, and multiple neurological disorder, various radiation sources etc. all may cause B, T, K, NK immunocyte genetic mutation or " ossifing ", gene to this " ossifing ", be referred to as in the present invention " recessive gene ", this recessive gene can not be in time, express accurately, pathogenic microorganism and multiple virulence factor can not be resisted timely to external world, and human body can be ill.

Years of researches of the present invention are found, various immunogenes are in vital movement process separately, one species specific " life ripple " launched in the capital, " life ripple " difference that different immunogenes is launched, immunogene is made immune response by these " life ripples " transmission immunologic information to the injurious factor of infringement human body. Therefore when immunogene changed, " the life ripple " launched will change, and that pipe is that a kind of small variation all can cause the change of " life ripple ". Key of the present invention has been to find that gene " life ripple " can cause change because of injurious factor, but also can under with the regulation and control of useful life ripple material, recover normal, the life ripple that therefore can use the useful factor to form activates and is caused " recessive gene " of " ossifing " by injurious factor, makes it recover normal.

The present invention is according to above principle, adopt the artificial electric wave of simulation human body immunogene " life ripple ", create a kind of " the useful factor ", and this useful factor material made biologic product, regulate and control the immunogene of variation with this biologic product, thereby reach the effect of regulating immunologic function. By the regulation and control immunologic function, make human body strengthen the ability of resisting disease, make the fast quick-recovery of human body of suffering from multiple sufferer.

The present invention thinks by a large amount of research, obtain a kind of active material that can regulate and control immunogene by manual simulation's " life ripple ", is a very difficult job. The present invention was through the exploration discovery in more than 20 years, and the microorganism human use's that occurring in nature is ubiquitous only is " a drop in the ocean ", this be because people to the microbial gene function understand few. Even to this day, the human microorganism of understanding the full gene constitute and function only has 26 kinds, and these 26 kinds of microorganisms also only are kinds simple in structure, does not also understand for the eukaryotic microorganisms mankind of complexity. Particularly in the human yeast microorganism that often uses, contain the gene that is not utilized in a large number, these genes have become " gene ossifys " owing to can not get for a long time using, and the present invention claims that these " gene ossifys " are " latent function gene ". What is more important exists required for the present invention wanting in these " latent function genes ", can be used in the useful protein matter of the human body immune function of regulation and control. The present invention utilize the method for manual simulation's life ripple activate in the yeast can expression regulation body immune function protein substance " latent function gene " realize.

The present invention adopts simulation life wave method to activate yeast " latent function gene ", has cultivated 4 kinds of specific yeasts that activate respectively B, T, K, NK4 kind immunocyte, has made the biologic product of regulation and control body immune functions. The present invention is in order to make this 4 species specific yeast, and is edible rear smoothly by stomach, and guarantees can not killed by a large amount of acidic materials under one's belt, and the present invention has done this 4 species specificity yeast again the condition of anti-PH≤2.5 and cultivated. Yeast cells after the cultivation will arrive small intestine by the stomach organ smoothly. The specific yeast cell is cleaved under the effect of small intestine plurality of enzymes, discharges to be packaged in intracellular specific immune function regulatory enzyme (albumen). The expression of immunogene in the activation of these specific immune function regulatory enzymes " selectivity ", the corresponding immunocyte of regulation and control human body. 4 kinds of human body immunological regulation specific yeasts involved in the present invention because they all are the human long-term edible or upper microorganisms of using of production, can not produce any toxic and side effect. The organized enzyme that discharges in these specific yeast cells, have the moment activation, regulate the effect that the interior immunogene of human body is correct, efficiently express, then will be absorbed along with the metabolism in the human body loses the nutriment that activity is transformed into human body very soon, residual in can not causing in human body.

The mechanism of action of regulation and control body immune function biologic product involved in the present invention brief description is as follows: the nucleus in the regulation and control body immune function biologic product be 4 kinds with the albumen of different frequency characteristic and voltage strength " life ripple ", these the 4 kinds albumen with different frequency characteristic and voltage strength " life ripple ", have respectively the function that activates, regulates and control and proofread and correct human body B, T, K, NK immunogene, these 4 kinds of albumen contain respectively again in 4 species specificity yeast. This 4 primary yeast is exactly the microorganism that the present invention adopts manual simulation's " life ripple " to activate, and claims that in the present invention these the 4 kinds yeast that simulated " life ripple " activation " latent function gene " are specific yeast. Contain respectively the immunoloregulation function enzyme that is activated in this 4 species specificity yeast cells, these immunoloregulation function enzymes, the function of B immunocyte, T immunocyte, K immunocyte and NK immunocyte immunogene in the narrow spectrum activation human body, make abnormal immunogene recover, activate and proofread and correct, and efficient correction. These specific yeast are cultivated out by separately specific simulation " life ripple " condition. Activation human body B cellular immune function specific yeast used in the present invention, employed manual simulation's wave frequency and received-signal strength are to be determined by the immunogene specificity " life ripple " of immune B cell; Activate human body T cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life ripple " by immune t-cell determines; Activate human body K cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life ripple " by immune K cell determines; Activate human body NK cellular immune function specific yeast, employed manual simulation's wave frequency and received-signal strength are that the immunogene specificity " life ripple " by immune NK cell determines. The specific yeast of these 4 kinds of difference in functionalitys activates, simulates " life ripple " condition through recessive gene and cultivates, and makes and has produced the organized enzyme (albumen) that has activation, regulates and control above-mentioned 4 kinds of immunogenes in the cell. When using this 4 species specificity yeast preparation, specific yeast enters in the small intestine of human body, lysis under the effect of small intestine plurality of enzymes, and the cell after the cracking discharges immunogene and activates the regulation and control enzyme. These immunogenes activate regulation and control enzymes and are entered blood by intestinal absorption immediately, are transported to respectively in 4 kinds of immunocytes by blood, thereby reach the purpose of activations, 4 kinds of immunogene corrections raisings of regulation and control immunity.

Immunologic function biological regulator of the present invention is that the step by following two aspects realizes. First step is to adopt specific simulation " life ripple " to activate common yeast, the specific yeast that these yeast is become have different adjustment B, T, K, NK cellular immune function, and these yeast are cultivated through anti-low PH condition; Second step is under the condition of special simulation life ripple, enlarges to cultivate these specific yeasts, then these specific yeasts is made the biological products that the regulation and control immunologic function is used. The implementation process of these two aspects is to realize by following step. Five, simulation " life ripple "---activate yeast function " recessive gene " method program

In above narration, illustrated that the present invention adopts common yeast commonly used in the life of simulation " life ripple " method activation of human, make in these yeast cells, " the latent function gene " that can not express under normal condition brings back to life, and the gene after the resurrection (did not become) active gene that efficiently expresses under given conditions by originally not expressing. The active gene that these become, give expression to respectively have that selectivity activates, regulation and control and proofread and correct the immunogene of immunogene, the improper expression of K cell of immunogene, the improper expression of T cell of the improper expression of B cell and the albumen of the immunogene function of the improper expression of NK cell. As everyone knows, common yeast is the zymophyte of the many kinds of substances such as class fermentation starch, carbohydrate, protide, multiplex bacterial classification in brewing alcoholic beverages, make bread, make various food, make medicine and multiple product thereof, although saccharomycetic various in style, Various Functions, but never the people utilizes the expressed specific proteins of yeast, activate respectively, regulate and proofread and correct the immunogene function that B, T, K, NK can not normal expressions, to carry out recessive gene in the method for not using patent of the present invention be not possess above-mentioned functions before activating to these yeast cells certainly.

Five-1, activate the method step that is used for regulation and control B cellular immune function gene yeasts " latent function gene ":

A large amount of gene technology studies have shown that a complete B cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of B cellular immune function gene, and another part is the structural gene of B cellular immune function gene. The structural gene of B cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control B cellular immune function gene of B cellular immune function gene. Therefore, keep the character of the expressed enzyme of B cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of B cellular immune function gene constant, namely can not change the base sequence of gene; The structural gene that reaches B cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of B cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life ripple " method, obtains to have the specific yeast of expression regulation B cellular immune function gene protein.

Consult accompanying drawing two, the method step of employing manual simulation involved in the present invention " life ripple " activation yeast " latent function gene " is as follows:

1. according to the one-tenth assignment system culture medium of subordinate list one, and sterilization treatment.

2. select suitable yeast specie (can select yeast to see attached list three), according to active yeast cell/culture medium 〉=1 * 10⁸The ratio of individual/1000ml is injected the culture medium in the container shown in the accompanying drawing two.

3. the temperature in the maintenance container was cultivated 24-56 hour between 37 ± 5 ℃.

4. open the electric wave transmitter of the life of simulation shown in the accompanying drawing two ripple, will export wave frequency and be adjusted in 9142 ± 0.6MHz scope.

5. electric wave transmitter output radio wave field strength degree is adjusted to: in 230 ± 15mv/cm scope.

6. the blake bottle of Yeast Cultivation liquid will be housed, be installed to the receiver amplifier output according to the pattern shown in the accompanying drawing two, receive frequency will be adjusted on the frequency consistent with transmitter institute tranmitting frequency. The distance of regulating simultaneously between generator and the receiving instrument is 100 ± 20cm, and { be 100cm such as distance, the output received-signal strength of its emitter is: (230 ± 15mv/cm) * 100=21.5-24.5v} to require to calculate the output received-signal strength of emitter according to determined distance according to 230 ± 15mv/cm.

7. under these conditions, and keep 37 ± 5 ℃ temperature conditions, activate 42-72 hour.

8. yeast cells after above condition activates adopts the methods such as vacuum freeze drying or low temperature drying to make the preservations such as ampoule, pulvis.

Example one: activate the method that is used for regulation and control B cellular immune function gene yeasts " latent function gene " and be exemplified below:

1. according to the one-tenth assignment system culture medium 1000-2000ml of subordinate list one, and sterilization treatment.

2. select the IFFI1021 yeast specie, according to IFFI1021 cell/culture medium 〉=1 * 10 of living⁸The ratio of individual/1000ml is injected the culture medium in the container shown in the accompanying drawing two.

4. open the electric wave transmitter shown in the accompanying drawing two, will export wave frequency and be adjusted in 9142 ± 0.6MHz scope.

5. electric wave transmitter output received-signal strength is adjusted to: in 21.5-24.5V (take distance as 100cm as the example) scope.

6. the blake bottle of yeast IFFI1021 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the accompanying drawing two, receive frequency and the tranmitting frequency of transmitter will be adjusted in identical 9142 ± 0.6MHz scope. The distance of regulating simultaneously between generator and the receiving instrument is 100cm.

8. IFFI1021 yeast cells after above condition activates adopts the methods such as vacuum freeze drying or low temperature drying to make the preservations such as ampoule, pulvis.

Five-2, activate the method step that is used for modulating T cell immunologic function gene yeast " latent function gene ":

A large amount of gene technology studies have shown that a complete T cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of T cellular immune function gene, and another part is the structural gene of T cellular immune function gene. The structural gene of T cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control T cellular immune function gene of T cellular immune function gene. Therefore, keep the character of the expressed enzyme of T cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of T cellular immune function gene constant, namely can not change the base sequence of gene; The structural gene that reaches T cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of T cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life ripple " method, obtains to have the specific yeast of expression regulation T cellular immune function gene protein.

4. open the electric wave transmitter shown in the accompanying drawing two, will export wave frequency and be adjusted in 8998 ± 0.5MHz scope.

5. electric wave transmitter output received-signal strength is adjusted to: in 230 ± 15mv/cm scope.

Example two: activate the method that is used for modulating T cell immunologic function gene yeast " latent function gene " and be exemplified below:

2. select the IFFI1212 yeast specie, according to IFFI1212 cell/culture medium 〉=1 * 10 of living⁸The ratio of individual/1000ml is injected the culture medium in the container shown in the accompanying drawing two.

6. the blake bottle of yeast IFFI1212 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the accompanying drawing two, receive frequency and the tranmitting frequency of transmitter will be adjusted in identical 8998 ± 0.5MHz scope. The distance of regulating simultaneously between generator and the receiving instrument is 100cm.

8. IFFI1212 yeast cells after above condition activates adopts the methods such as vacuum freeze drying or low temperature drying to make the preservations such as ampoule, pulvis. Five-3, activate the method step that is used for regulation and control K cellular immune function gene yeasts " latent function gene ":

A large amount of gene technology studies have shown that a complete K cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of K cellular immune function gene, and another part is the structural gene of K cellular immune function gene. The structural gene of K cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control K cellular immune function gene of K cellular immune function gene. Therefore, keep the character of the expressed enzyme of K cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of K cellular immune function gene constant, namely can not change the base sequence of gene; The structural gene that reaches K cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of K cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life ripple " method, obtains to have the specific yeast of expression regulation K cellular immune function gene protein.

4. open the electric wave transmitter shown in the accompanying drawing two, will export wave frequency and be adjusted in 9563 ± 0.5MHz scope.

Example three: activate the method that is used for regulation and control K cellular immune function gene yeasts " latent function gene " and be exemplified below:

2. select the IFFI1301 yeast specie, according to IFFI1301 cell/culture medium 〉=1 * 10 of living⁸The ratio of individual/1000ml is injected the culture medium in the container shown in the accompanying drawing two.

6. the blake bottle of yeast IFFI1301 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the accompanying drawing two, receive frequency and the tranmitting frequency of transmitter will be adjusted in identical 9563 ± 0.5MHz scope. The distance of regulating simultaneously between generator and the receiving instrument is 100cm.

7. under these conditions, and keep 37 ± 5 ℃ temperature conditions, activate 42-72 hour. 8. IFFI1301 yeast cells after above condition activates adopts the methods such as vacuum freeze drying or low temperature drying to make the preservations such as ampoule, pulvis. Five-4. activate the method step that is used for regulation and control NK cellular immune function gene yeasts " latent function gene ":

A large amount of gene technology studies have shown that a complete NK cellular immune function gene forms (seeing accompanying drawing one) by the two large divisions, and a part is the promotor gene of NK cellular immune function gene, and another part is the structural gene of NK cellular immune function gene. The structural gene of NK cellular immune function gene has determined its expressed immunoenzymatic character; The expressed immunoenzymatic quantity of structural gene, immunocompetence and immune response ability, i.e. immunoenzymatic the tiring of the promotor gene control NK cellular immune function gene of NK cellular immune function gene. Therefore, keep the character of the expressed enzyme of NK cellular immune function gene constant, must manage to keep the structural gene dna sequence dna of NK cellular immune function gene constant, namely can not change the base sequence of gene; The structural gene that reaches NK cellular immune function gene efficiently expresses, and keeps the best immunocompetence of expressed immuno-enzymatic and immune response ability timely, must manage to make the promotor gene of NK cellular immune function gene efficiently to start. The present invention activates yeast " latent function gene " by manual simulation's " life ripple " method, obtains to have the specific yeast of expression regulation NK cellular immune function gene protein.

4. open the electric wave transmitter shown in the accompanying drawing two, will export wave frequency and be adjusted in 9122 ± 0.5MHz scope.

Example four: the method that activate to be used for regulation and control NK cellular immune function gene yeasts " latent function gene " is exemplified below: 1. according to the one-tenth assignment system culture medium 1000-2000ml of subordinate list one, and sterilization treatment.

2. select the IFFI1048 yeast specie, according to IFFI1048 cell/culture medium 〉=1 * 10 of living⁸The ratio of individual/1000ml is injected the culture medium in the container shown in the accompanying drawing two.

6. the blake bottle of yeast IFFI1048 nutrient solution will be housed, be installed to the receiver amplifier output according to the pattern shown in the accompanying drawing two, receive frequency and the tranmitting frequency of transmitter will be adjusted in identical 9122 ± 0.5MHz scope. The distance of regulating simultaneously between generator and the receiving instrument is 100cm.

7. under these conditions, and keep 37 ± 5 ℃ temperature conditions, activate 42-72 hour. 8. IFFI1048 yeast cells after above condition activates adopts the methods such as vacuum freeze drying or low temperature drying to make the preservations such as ampoule, pulvis. Experiment for example experiment gives an example one: a. gets 60 of wates rats, is divided into totally 3 groups of A, B, C, every group of 20 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group. B. adopt 180 ascites tumors, big white mouse is respectively organized in inoculation respectively. C. from postvaccinal second day, A organizes that (it is identical to contain 4 species specificity number of yeast cells, is 〉=1 * 10 to this biologic product liquid⁸Individual/ml, i.e. 4 species specificity yeast cells total amount 〉=4 * 10⁸Individual/ml), dosage is 0.3-0.6ml/kg; B organizes to endoxan, and dosage is 10-20ug/kg; C organizes to physiological saline 0.3-0.6ml/kg. Once a day. D. dissect to detect afterwards the size of ascites tumor in seven days, such as following table:

Annotate: on show listed-27.2% ,-86.4%, respectively expression (B) control group and (A) percentage amounts that reduces than blank group (CK) knurl body average weight of test group knurl body average weight. Experiment gives an example two: a. gets 90 of wates rats, is divided into totally 3 groups of A, B, C, every group of 30 rats. The A group is experimental group, and the B group is for using the cyclophosphamide-a control group, and C is the blank group. B. adopt the U-14 solid tumor, big white mouse is respectively organized in inoculation respectively. C. from postvaccinal second day, A organizes that (it is identical to contain 4 species specificity number of yeast cells, is 〉=1 * 10 to this biologic product liquid⁸Individual/ml, i.e. 4 species specificity yeast cells total amount 〉=4 * 10⁸Individual/ml), dosage is 0.3-0.6ml/kg; B organizes to endoxan, and dosage is 10-20ug/kg; C organizes to physiological saline 0.3-0.6ml/kg. Once a day. D. take the size that dissect to detect afterwards ascites tumor in 14 days, such as following table:

Annotate: on show listed-10.5% ,-89.5%, respectively expression (B) control group and (A) percentage amounts that reduces than blank group (CK) knurl body average weight of test group knurl body average weight. Six, the domestication of specific yeast acidproof (anti-low PH)

Illustrated that more than the present invention adopts the method for manual simulation's " life ripple ", activate respectively yeast difference " latent function gene ", obtain 4 kinds of specific yeasts that are respectively applied to activate, regulate and control B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene function. But this 4 species specificity yeast can't be directly used in the various immunogenes of regulation and control, and this is because they can't adapt to the interior environment of human body. As everyone knows, be a very complex environment in the human body, and various envirment factor is all changing all the time. When this 4 species specific yeast enters the approach of small intestine by oral cavity, stomach, be difficult to ensure the integrality of its cell, more therefore the activity of purpose enzyme in the Customers ' Legal Right yeast cells ensures that the yeast cells activity is very crucial. The present invention has adopted the envirment factor domestication of 4 species specificity yeast has been cultivated, and the domestication and culture method step is as follows:

The yeast environmental suitability domestication of the present invention's 4 species specificity functions, realize by method shown in figure three: consult accompanying drawing three, the method step of the present invention's 4 species specificity yeast environmental suitabilities domestication is respectively described below: the specific yeast step of (), domestication regulation and control B cellular immunity gene

1. the method according to subordinate list two configures culture medium, and sterilization treatment.

2. get the culture medium 1000ml that configures in 1., be injected in the container of accompanying drawing three.

3. the species specificity yeast juice 10ml that gets regulation and control B cellular immunity gene (contains active dry yeasr cell 〉=1 * 10⁸Individual/as ml), to inject the container shown in the accompanying drawing three.

4. open the wave generator shown in figure three, and be adjusted on the species specificity yeast selectivity frequency 9142 ± 0.6MHz that is adapted to B cellular immunity gene.

5. the electric wave output voltage of regulating shown in figure three is 5-10mv/ml (the employed received-signal strength of 1000ml culture medium is 5-10v).

6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (2), the specific yeast step of domestication modulating T cell immunogene

3. the species specificity yeast juice 10ml that gets the modulating T cell immunogene (contains active dry yeasr cell 〉=1 * 10⁸Individual/as ml), to inject the container shown in the accompanying drawing three.

4. open the wave generator shown in figure three, and be adjusted on the species specificity yeast selectivity frequency 8998 ± 0.5MHz that is adapted to the modulating T cell immunogene.

6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (3), the specific yeast step of domestication regulation and control K cellular immunity gene

3. the species specificity yeast juice 10ml that gets regulation and control K cellular immunity gene (contains active dry yeasr cell 〉=1 * 10⁸Individual/as ml), to inject the container shown in the accompanying drawing three.

4. open the wave generator shown in figure three, and be adjusted on the species specificity yeast selectivity frequency 9563 ± 0.6MHz that is adapted to K cellular immunity gene.

6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. (4), the specific yeast step of domestication regulation and control NK cellular immunity gene

3. the species specificity yeast juice 10ml that gets regulation and control NK cellular immunity gene (contains active dry yeasr cell 〉=1 * 10⁸Individual/as ml), to inject the container shown in the accompanying drawing three.

4. open the wave generator shown in figure three, and be adjusted on the species specificity yeast selectivity frequency 9122 ± 0.6MHz that is adapted to NK cellular immunity gene.

6. keep above-mentioned wave frequency and received-signal strength constant, after 37 ± 5 ℃ temperature conditions are cultivated 48-96 hour, separate under the condition that is kept at 0-4 ℃ for subsequent use. Seven, the culture process of method for making of the present invention and 4 species specificity yeast

The method for making of biologic product of the present invention mainly is divided into the cultivation, mixing of specific yeast, the master operation such as concentrated, canned, sees also Figure of description four.

Specific yeast involved in the present invention is cultivated totally 4 kinds. This 4 species specificity yeast only is to have obtained seed after obtaining through said method, make a large amount of biologic products of the present invention, and the specific yeast of capacity must be arranged, and therefore needs to enlarge and cultivates. The present invention sends into the hybrid technique of 4 primary yeast liquid respectively through 4 species specificity yeast juices of above specific yeast culture process acquisition. 4 species specificity Yeast Cultivation are to distinguish by the following method step realization: the specific yeast culture process of seven-1, regulating B cellular immunity gene function

The specific yeast culture process engineering of adjusting B cellular immune function involved in the present invention is as shown in the accompanying drawing five.

Consult Figure of description five of the present invention, the specific yeast culture process step of adjusting B cellular immune function involved in the present invention is as follows:

1. according to the one-tenth assignment system culture medium of subordinate list four, and after sterilization treatment, be injected into respectively in accompanying drawing five A, B, the C tank.

2. will activate through manual simulation's life wave method, and the specific yeast of the adjusting B cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank A shown in the accompanying drawing five, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.

3. adjusting wave generator, making it export biological electric wave is 9142 ± 0.6MHz, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).

4. keep in the constant situation of above-mentioned wave frequency and received-signal strength, cultivated 56-72 hour under 37 ± 5 ℃ of conditions.

5. when the specific yeast living cells of regulating B cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique. Seven-2, regulate the specific yeast culture process of T cellular immunity gene function

The specific yeast culture process engineering of adjusting T cellular immune function involved in the present invention is as shown in the accompanying drawing five.

Consult Figure of description five of the present invention, the specific yeast culture process step of adjusting T cellular immune function involved in the present invention is as follows:

2. will activate through manual simulation's life wave method, and the specific yeast of the adjusting T cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank A shown in the accompanying drawing five, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.

3. adjusting wave generator, making it export biological electric wave is 8998 ± 0.5MHz, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).

5. when the specific yeast living cells of regulating T cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique. Seven-3, regulate the specific yeast culture process of K cellular immunity gene function

The specific yeast culture process engineering of adjusting K cellular immune function involved in the present invention is as shown in the accompanying drawing five.

Consult Figure of description five of the present invention, the specific yeast culture process step of adjusting K cellular immune function involved in the present invention is as follows:

2. will activate through manual simulation's life wave method, and the specific yeast of the adjusting K cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank A shown in the accompanying drawing five, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.

3. adjusting wave generator, making it export biological electric wave is 9563 ± 0.5MHz, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).

5. when the specific yeast living cells of regulating K cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique. Seven-4, regulate the specific yeast culture process of NK cellular immunity gene function

The specific yeast culture process engineering of adjusting NK cellular immune function involved in the present invention is as shown in the accompanying drawing five.

Consult Figure of description five of the present invention, the specific yeast culture process step of adjusting NK cellular immune function involved in the present invention is as follows:

2. will activate through manual simulation's life wave method, and the specific yeast of the adjusting NK cellular immune function that obtains of the cultivation through tolerating low PH (less than PH2.5), be input among the seeding tank A shown in the accompanying drawing five, as seed liquor. Then A tank seed liquor is injected into enlarge in the culture medium of B tank according to certain ratio and cultivates, the ratio of injection is: A seed liquor/B nutrient solution=5ml/1000ml.

3. adjusting wave generator, making it export biological electric wave is 9122 ± 0.5MHz, and calculate according to the requirement of 0.5-1.0v/L simultaneously, the setting received-signal strength (quantity such as nutrient solution in the hypothesis B tank is 50L, and single magnetic wave intensity should be: 0.5-1.0v/L * 50L=25v-50v).

5. when the specific yeast living cells of regulating NK cellular immunity gene in the B tank reaches 2,000,000,000/ml, in B tank yeast juice input C tank, prepare to be transported to lower road hybrid technique. Eight, the hybrid technique of 4 species specificity yeast

4 species specificity yeast juice hybrid techniques involved in the present invention are to carry out according to the ratio of following table.

Specific yeast liquid mixed proportion list (in the 4000L mixed liquor)

Specific yeast liquid kind	Quantity	Ratio	Requirement
Specific yeast liquid kind	Quantity	Ratio	Requirement	Regulation and control B cellular immunity gene function yeast juice	1000L	25％	In nutrient solution
Modulating T cell genetic immunization function yeast juice	1000L	25％	In nutrient solution		1000L	25％	In nutrient solution
Modulating T cell genetic immunization function yeast juice	1000L	25％	In nutrient solution	Regulation and control K cellular immunity gene function yeast juice	1000L	25％	In nutrient solution
Regulation and control NK cytogene immunologic function yeast juice	1000L	25％	In nutrient solution		1000L	25％	In nutrient solution

The mixing of specific yeast liquid of the present invention is to carry out according to the mode of accompanying drawing six. Consult accompanying drawing six, this technique realizes by following steps:

1. 4 species specificity yeast juices are input to respectively in A, B, C, the D tank.

2. 4 species specificity yeast juices in A, B, C, the D tank are input among the blending tank M according to the ratio of equivalent and mix.

3. mixed yeast juice is injected in the concentration technology shown in the accompanying drawing seven, prepares concentrated. Nine, specific yeast concentration technology

Concentration technology is with above-mentioned eight. In the mixed liquid concentration of 4 species specificity yeast juices, concentrated purpose is that the requirement when reaching the embedding finished product is set. Concentration technology is divided into two-stage, and it is concentrated to be transported to the second level after the first order is concentrated again, and finally reaching enrichment is about 64%.

Concentrating of specific yeast liquid involved in the present invention, realize according to following steps:

1. will through the mixed mixed liquor of above-mentioned hybrid technique, be transported to the concentration technology first order thickener equipment shown in the accompanying drawing seven.

In first order concentration tank with the specific yeast mixed liquor, be concentrated into 80% (measurement basis calculation), then be transported in the thickener of the second level concentrated. Concentrated can adopt that freezing vacuum is concentrated, the normal temperature partial vacuum is concentrated or the method such as concentrated of heating, but which kind of concentrated mode all must be take the activity that keeps the specific yeast cell as benchmark.

3. again be concentrated into 80% in the concentration tank of the second level, relevant concentrated requirement is still with the condition in 2.. Be transported to immediately dosing technology after concentrated and prepare potting. Ten, dosing technology

Dosing technology involved in the present invention is divided into cooling, metering and three processes of embedding. The purpose of cooling is that the temperature rise that causes in the concentration process will be got off, in order to avoid produce gas after the embedding; Metering is to concentrate the control requirement that whether reaches total amount in order to check, and prepares for the used bottle quantity of embedding; Embedding is the final step of specific yeast liquid being made the finished product main technique. This technique is to realize according to the step shown in the accompanying drawing eight:

1. the specific yeast mixed liquor after will concentrating is transported in the cooling tank shown in the accompanying drawing eight, is cooled to 12-15 ℃.

2. will through the mixed liquor of cooling tank cooling, be transported in the measuring tank and measure. Mixed liquor after the metering is transported to the embedding of embedding machine, makes finished product. Subordinate list one

Activate regulation and control (B, T, K, NK) cell used yeast " latent function gene " medium component table

Medium component	Quantity
Medium component	Quantity	Sweet mellow wine	16g
K ₂HPO ₄	0.25g	Sweet mellow wine	16g
K ₂HPO ₄	0.25g	MgSO ₄·7H ₂O	0.2g
NaCL	0.22g	MgSO ₄·7H ₂O	0.2g
NaCL	0.22g	CaSO ₄·H ₂O	0.5g
CaCO ₃	6.0g	CaSO ₄·H ₂O	0.5g
CaCO ₃	6.0g	Urea	0.2-0.5g
Serum	100-300ml	Urea	0.2-0.5g
Serum	100-300ml	Distilled water	700-900mL

Subordinate list two environmental condition adaptability medium component tables (take 1000ml as example)

Medium component	Quantity	Explanation
Medium component	Quantity	Explanation	Wild Jujube Juice	300ml	With the clear liquid that dry acid jujube/water=the 1g/5ml ratio is made
The sub-juice of mountain fourth	500ml	With the dried mountain clear liquid that fourth/water=the 1g/5ml ratio is made	Wild Jujube Juice	300ml
The sub-juice of mountain fourth	500ml		(NH ₄) ₂SO ₄	0.25g
K ₂HPO ₄	0.2g		(NH ₄) ₂SO ₄	0.25g
K ₂HPO ₄	0.2g		MgSO ₄·7H ₂O	0.22g
NaCL	0.5g		MgSO ₄·7H ₂O	0.22g
NaCL	0.5g		CaSO ₄·2H ₂O	0.3g
CaCO ₃	3.0g		CaSO ₄·2H ₂O	0.3g
CaCO ₃	3.0g		Specific yeast nutrient solution after the activation	Each 20ml	Contain active yeast cell 〉=1 * 10⁸Individual/ml

Subordinate list three

The microbe species that this patent is related

(showing listed microorganism but be not limited only to this)

The affiliated class-01 that belongs to	Saccharomyces cerevisiae Hansen saccharomyces cerevisiae
The affiliated class-01 that belongs to	Saccharomyces cerevisiae Hansen saccharomyces cerevisiae				Former purposes-01	Wine brewing, edible and food manufacturing
ACCC2034	ACCC2035	ACCC2036	ACCC2037	ACCC2038	Former purposes-01	Wine brewing, edible and food manufacturing
ACCC2034	ACCC2035	ACCC2036	ACCC2037	ACCC2038	ACCC2039	ACCC2040	ACCC2041	ACCC2042	AS2.1
AS2.4	AS2.11	AS2.14	AS2.16	AS2.56	ACCC2039	ACCC2040	ACCC2041	ACCC2042	AS2.1
AS2.4	AS2.11	AS2.14	AS2.16	AS2.56	AS2.69	AS2.70	AS2.93	AS2.98	AS2.101
AS2.109	AS2.110	AS2.112	AS2.139	AS2.173	AS2.69	AS2.70	AS2.93	AS2.98	AS2.101
AS2.109	AS2.110	AS2.112	AS2.139	AS2.173	AS2.174	AS2.182	AS2.196	AS2.242	AS2.336
AS2.346	AS2.369	AS2.374	AS2.375	AS2.379	AS2.174	AS2.182	AS2.196	AS2.242	AS2.336
AS2.346	AS2.369	AS2.374	AS2.375	AS2.379	AS2.380	AS2.382	AS2.390	AS2.393	AS2.395
AS2.396	AS2.397	AS2.398	AS2.399	AS2.400	AS2.380	AS2.382	AS2.390	AS2.393	AS2.395
AS2.396	AS2.397	AS2.398	AS2.399	AS2.400	AS2.406	AS2.408	AS2.409	AS2.413	AS2.414
AS2.415	AS2.416	AS2.422	AS2.423	AS2.430	AS2.406	AS2.408	AS2.409	AS2.413	AS2.414
AS2.415	AS2.416	AS2.422	AS2.423	AS2.430	AS2.431	AS2.432	AS2.451	AS2.452	AS2.453
AS2.458	AS2.460	AS2.463	AS2.467	AS2.486	AS2.431	AS2.432	AS2.451	AS2.452	AS2.453
AS2.458	AS2.460	AS2.463	AS2.467	AS2.486	AS2.501	AS2.502	AS2.503	AS2.504	AS2.516
AS2.535	AS2.536	AS2.558	AS2.560	AS2.561	AS2.501	AS2.502	AS2.503	AS2.504	AS2.516
AS2.535	AS2.536	AS2.558	AS2.560	AS2.561	AS2.562	AS2.576	AS2.593	AS2.594	AS2.614
AS2.620	AS2.628	AS2.631	AS2.666	AS2.982	AS2.562	AS2.576	AS2.593	AS2.594	AS2.614
AS2.620	AS2.628	AS2.631	AS2.666	AS2.982	AS2.1190	AS2.1364	AS2.1396	IFFI1001	IFFI1002
IFFI1005	IFFI1006	IFFI1008	IFFI1009	IFFI1010	AS2.1190	AS2.1364	AS2.1396	IFFI1001	IFFI1002
IFFI1005	IFFI1006	IFFI1008	IFFI1009	IFFI1010	IFFI1012	IFFI1021	IFFI1027	IFFI1037	IFFI1042
IFFI1043	IFFI1045	IFFI1048	IFFI1049	IFFI1050	IFFI1012	IFFI1021	IFFI1027	IFFI1037	IFFI1042
IFFI1043	IFFI1045	IFFI1048	IFFI1049	IFFI1050	IFFI1052	IFFI1059	IFFI1060	IFFI1063	IFFI1202
IFFI1203	IFFI1206	IFFI1209	IFFI1210	IFFI1211	IFFI1052	IFFI1059	IFFI1060	IFFI1063	IFFI1202
IFFI1203	IFFI1206	IFFI1209	IFFI1210	IFFI1211	IFFI1212	IFFI1213	IFFI1214	IFFI1215	IFFI1220
IFFI1221	IFFI1224	IFFI1247	IFFI1248	IFFI1251	IFFI1212	IFFI1213	IFFI1214	IFFI1215	IFFI1220
IFFI1221	IFFI1224	IFFI1247	IFFI1248	IFFI1251	IFFI1270	IFFI1277	IFFI1287	IFFI1289	IFFI1290
IFFI1291	IFFI1292	IFFI1293	IFFI1297	IFFI12300	IFFI1270	IFFI1277	IFFI1287	IFFI1289	IFFI1290
IFFI1291	IFFI1292	IFFI1293	IFFI1297	IFFI12300	IFFI1301	IFFI1302	IFFI1307	IFFI1308	IFFI1309
IFFI1310	IFFI1311	IFFI1335	IFFI1336	IFFI1337	IFFI1301	IFFI1302	IFFI1307	IFFI1308	IFFI1309
IFFI1310	IFFI1311	IFFI1335	IFFI1336	IFFI1337	IFFI1338	IFFI1339	IFFI1340	IFFI1345	IFFI1348
IFFI1396	IFFI1397	IFFI1399	IFFI1411	IFFI1413	IFFI1338	IFFI1339	IFFI1340	IFFI1345	IFFI1348
IFFI1396	IFFI1397	IFFI1399	IFFI1411	IFFI1413	The affiliated class-02 that belongs to	Saccharomyces cerevisiae Hansen Var. ellipsoideus (Hansen) Dekker ellipse brewing yeast
Former purposes-02	Wine brewing, edible and food manufacturing				The affiliated class-02 that belongs to
Former purposes-02	Wine brewing, edible and food manufacturing				ACCC2043	AS2.2	AS2.3	AS2.8	AS2.53
AS2.163	AS2.168	AS2.483	AS2.541	AS2.559	ACCC2043	AS2.2	AS2.3	AS2.8	AS2.53
AS2.163	AS2.168	AS2.483	AS2.541	AS2.559	AS2.606	AS2.607	AS2.611	AS2.612
					AS2.606	AS2.607	AS2.611	AS2.612
					The affiliated class-03 that belongs to	Saccharomyces chevalieri Xue Guilliermond watt yeast
Former purposes-03	Wine brewing, edible and food manufacturing				The affiliated class-03 that belongs to	Saccharomyces chevalieri Xue Guilliermond watt yeast

AS2.131	AS2.213
AS2.131	AS2.213			The affiliated class-04 that belongs to	Saccharomyces delbmeckii Dare cloth yeast
Former purposes-04	Food fermentation			The affiliated class-04 that belongs to	Saccharomyces delbmeckii Dare cloth yeast
Former purposes-04	Food fermentation			AS2.285
The affiliated class-05 that belongs to	Saccharomyces delbrueckii Lindner ver.mongolicus (Saito) Lodder et van Rij Mongolia Dare cloth yeast			AS2.285
The affiliated class-05 that belongs to				Former purposes-05	Food fermentation
AS2.209	AS2.1157			Former purposes-05	Food fermentation
AS2.209	AS2.1157			The affiliated class-06 that belongs to	Saccharomyces exiguous Hansen saccharomyces exiguus
Former purposes-06	Food fermentation			The affiliated class-06 that belongs to	Saccharomyces exiguous Hansen saccharomyces exiguus
Former purposes-06	Food fermentation			AS2.349	AS2.1158
The affiliated class-07 that belongs to	Saccharomyces fermentati (Saito) Lodder et van Rij saccharomyces fermentati			AS2.349	AS2.1158
The affiliated class-07 that belongs to				Former purposes-07	Food fermentation
AS2.286	AS2.343			Former purposes-07	Food fermentation
AS2.286	AS2.343

				The affiliated class-08 that belongs to	Saccharomyces Logos van laer et Denamur ex Jorgensen Lip river lattice yeast
Former purposes-08	The functions such as class, food fermentation make grape wine			The affiliated class-08 that belongs to
Former purposes-08				AS2.156	AS2.327	AS2.335
The affiliated class-08 that belongs to	Saccharomyces mellis (Fabian et Quinet) Lodder et kreger van Rij saccharomyces mellis			AS2.156	AS2.327	AS2.335
The affiliated class-08 that belongs to				Former purposes-08	With carbohydrate, starch based fermentation
AS2.195				Former purposes-08	With carbohydrate, starch based fermentation
AS2.195				The affiliated class-09 that belongs to	The little saccharomyces ellipsoideus of Saccharomyces mellis Microellipsoides Osterwalder
Former purposes-09	With carbohydrate, starch based fermentation			The affiliated class-09 that belongs to
Former purposes-09	With carbohydrate, starch based fermentation			AS2.699
The affiliated class-10 that belongs to	Saccharomyces oviformis Osteralder ellipsoideus yeast			AS2.699
The affiliated class-10 that belongs to	Saccharomyces oviformis Osteralder ellipsoideus yeast			Former purposes-10	With carbohydrate, starch based fermentation
AS2.100				Former purposes-10	With carbohydrate, starch based fermentation
AS2.100				The affiliated class-11 that belongs to	Saccharomyces rosei (Guilliermond) Lodder et Kreger van Rij Luo Si yeast
Former purposes-11	With carbohydrate, starch based fermentation			The affiliated class-11 that belongs to
Former purposes-11	With carbohydrate, starch based fermentation			AS2.287
				AS2.287

The affiliated class-12 that belongs to	Saccharomyces rouxii Boutroux Lu Shi yeast
The affiliated class-12 that belongs to	Saccharomyces rouxii Boutroux Lu Shi yeast			Former purposes-12	With carbohydrate, starch based fermentation, make edible paste, soy sauce etc.
AS2.178	AS2.180	AS2.370	AS2.371	Former purposes-12

The affiliated class-13 that belongs to	Saccharomyces Sake Yabe saccharomyces sake
The affiliated class-13 that belongs to	Saccharomyces Sake Yabe saccharomyces sake				Former purposes-13	With carbohydrate, starch based fermentation
ACCC2045					Former purposes-13	With carbohydrate, starch based fermentation
ACCC2045					The affiliated class-14 that belongs to	Candida arborea Candida arborea
Former purposes-14	Be used for feed, amino acids manufacturing, cellulose, starch, carbohydrate, protein fermentation etc.				The affiliated class-14 that belongs to	Candida arborea Candida arborea
Former purposes-14					AS2.566
The affiliated class-15 that belongs to	Candida lambica (Lindner et Genoud) van.Uden et Buckley; Bright Bick Candida				AS2.566
The affiliated class-15 that belongs to					Former purposes-15	Be used for producing ester under the high temperature, make flavoring essence etc.
AS2.1182					Former purposes-15
AS2.1182					The affiliated class-16 that belongs to	Candida Krusei (Castellani) Berkhout Cruise yeast
Former purposes-16	Be used for wine brewing, make feed, amino acids, albumen etc.				The affiliated class-16 that belongs to	Candida Krusei (Castellani) Berkhout Cruise yeast
Former purposes-16					AS2.1045
					AS2.1045
					The affiliated class-17 that belongs to	Candida lipolytica (Harrison) Diddens et Lodder Candida lipolytica
Former purposes-17	Be used for oil dewaxing, make organic acid substance				The affiliated class-17 that belongs to
Former purposes-17	Be used for oil dewaxing, make organic acid substance				AS2.1207	AS2.1216	AS2.1220	AS2.1379	AS2.1398
AS2.1399	AS2.1400				AS2.1207	AS2.1216	AS2.1220	AS2.1379	AS2.1398
AS2.1399	AS2.1400				The affiliated class-17 that belongs to	The medium-sized level and smooth Candida of Candida parapsilosis (Ashford) Langeron et Talice Var.intermedia Van Rij et Verona
Former purposes-17	Utilize carbohydrate, starch based fermentation to make feed				The affiliated class-17 that belongs to
Former purposes-17					AS2.491
The affiliated class-18 that belongs to	Candida parapsilosis (Ashford) Langeron et Talice Candida parapsilosis				AS2.491
The affiliated class-18 that belongs to					Former purposes-18	Utilize pentose class hydrolyzate to make feed
AS2.590					Former purposes-18	Utilize pentose class hydrolyzate to make feed
AS2.590					The affiliated class-19 that belongs to	Candida pulcherriman (Lindner) Windisch iron oxide red yeast
Former purposes-19	Stimulating growth				The affiliated class-19 that belongs to
Former purposes-19	Stimulating growth				AS2.492
The affiliated class-20 that belongs to	Candida rugosa (Anderson) Diddens et Lodder fold candida				AS2.492
The affiliated class-20 that belongs to	Candida rugosa (Anderson) Diddens et Lodder fold candida				Former purposes-20	Oil dewaxing, production organic acid etc.
AS2.511	AS2.1367	AS2.1369	AS2.1372	AS2.1373	Former purposes-20	Oil dewaxing, production organic acid etc.
AS2.511	AS2.1367	AS2.1369	AS2.1372	AS2.1373	AS2.1377	AS2.1378	AS2.1384
The affiliated class-21 that belongs to	Candida tropicalis (Castellani) Berkhout candida tropicalis				AS2.1377	AS2.1378	AS2.1384
The affiliated class-21 that belongs to	Candida tropicalis (Castellani) Berkhout candida tropicalis				Former purposes-21	Carbohydrate fermentation; Cellulose, hemicellulose fermentation; Paper pulp already ferments; Sulfurous acid tobacco fermentation; Feed is made; Yeast extract, ergosterol manufacturing etc.
ACCC2004	ACCC2005	ACCC2006	AS2.164	AS2.402	Former purposes-21
ACCC2004	ACCC2005	ACCC2006	AS2.164	AS2.402	AS2.564	AS2.565	AS2.567	AS2.568	AS2.617
AS2.637	AS2.1387	AS2.1397			AS2.564	AS2.565	AS2.567	AS2.568	AS2.617

The affiliated class-22 that belongs to	Candida utilis Henneberg Lodder et Kreger Van Rij candida utili
The affiliated class-22 that belongs to					Former purposes-22	Eat and the feed manufacturing
AS2.120	AS2.281	AS2.1180			Former purposes-22	Eat and the feed manufacturing
AS2.120	AS2.281	AS2.1180			The affiliated class-23 that belongs to	The false capsule yeast of Crebrothecium ashbyii (Guilliermond) Routein=Eremothecium ashbyii Guilliermond A Shu
Former purposes-23	Be used for riboflavin manufacturing etc.				The affiliated class-23 that belongs to
Former purposes-23	Be used for riboflavin manufacturing etc.				AS2.481	AS2.482	AS2.1197
The affiliated class-24 that belongs to	Geotrichum candidum Link geotrichum candidum				AS2.481	AS2.482	AS2.1197
The affiliated class-24 that belongs to	Geotrichum candidum Link geotrichum candidum				Former purposes-24	Feed is made;
ACCC2016	AS2.361	AS2.498	AS2.616	AS2.1035	Former purposes-24	Feed is made;
ACCC2016	AS2.361	AS2.498	AS2.616	AS2.1035	AS2.1062	AS2.1080	AS2.1132	AS2.1175	AS2.1183
					AS2.1062	AS2.1080	AS2.1132	AS2.1175	AS2.1183
					The affiliated class-25 that belongs to	Hansenula anomala (Hansen) H et P sydow Hansenula anomala
Former purposes-25	Spices is made; Improve drinks fragrance, food flavor etc.;				The affiliated class-25 that belongs to	Hansenula anomala (Hansen) H et P sydow Hansenula anomala
Former purposes-25	Spices is made; Improve drinks fragrance, food flavor etc.;				ACCC2018	AS2.294	AS2.295	AS2.296	AS2.297
AS2.298	AS2.299	AS2.300	AS2.302	AS2.338	ACCC2018	AS2.294	AS2.295	AS2.296	AS2.297
AS2.298	AS2.299	AS2.300	AS2.302	AS2.338	AS2.339	AS2.340	AS2.341	AS2.470	AS2.592
AS2.641	AS2.642	AS2.782	AS2.635	AS2.794	AS2.339	AS2.340	AS2.341	AS2.470	AS2.592
AS2.641	AS2.642	AS2.782	AS2.635	AS2.794	The affiliated class-26 that belongs to	Hansenula arabitolenes Fang arabite Hansenula yeast
Former purposes-26	Produce the pure and mild glycerine of arabinose;				The affiliated class-26 that belongs to	Hansenula arabitolenes Fang arabite Hansenula yeast
Former purposes-26	Produce the pure and mild glycerine of arabinose;				AS2.887
The affiliated class-27 that belongs to	The outstanding fourth Hansenula yeast of Hansenula jadinii (A.et R Sartory Weill et Meyer) Wickerham				AS2.887
The affiliated class-27 that belongs to					Former purposes-27	Do not find the report of application
ACCC2019					Former purposes-27	Do not find the report of application
ACCC2019					The affiliated class-28 that belongs to	Hansenula saturnus (Klocker) H et P sydow Saturn Hansenula yeast
Former purposes-28	Do not find the report of application				The affiliated class-28 that belongs to
Former purposes-28	Do not find the report of application				ACCC2020
The affiliated class-29 that belongs to	Hansenula schneggii (Weber) Dekker Amur Hansenula yeast				ACCC2020
The affiliated class-29 that belongs to	Hansenula schneggii (Weber) Dekker Amur Hansenula yeast				Former purposes-29	Do not find the report of application
AS2.304					Former purposes-29	Do not find the report of application
AS2.304					The affiliated class-30 that belongs to	The inferior film Hansenula yeast of Hansenula subpelliculosa Bedford
Former purposes-30	From multiple liquor raw material processed or poor slag, obtain, but do not find the report of application				The affiliated class-30 that belongs to
Former purposes-30					AS2.740	AS2.760	AS2.761	AS2.770	AS2.783
AS2.790	AS2.798	AS2.866			AS2.740	AS2.760	AS2.761	AS2.770	AS2.783
AS2.790	AS2.798	AS2.866			The affiliated class-31 that belongs to	Kloeckera apiculata (Reess emend.Klocker) Janke lemon shape Ke Leke yeast
Former purposes-31	Do not find the report of application				The affiliated class-31 that belongs to

ACCC2022	ACCC2023	AS2.197	AS2.496	AS2.714
ACCC2022	ACCC2023	AS2.197	AS2.496	AS2.714	ACCC2021	AS2.711
The affiliated class-32 that belongs to	Lipomycess starkeyi Lodder et van Rij saccharomyces oleaginosus				ACCC2021	AS2.711
The affiliated class-32 that belongs to					Former purposes-32	Do not find the report of application
AS2.1390	ACCC2024				Former purposes-32	Do not find the report of application
AS2.1390	ACCC2024				The affiliated class-33 that belongs to	Pichia farinose (Lindner) Hansen pichia farinose
Former purposes-33	Do not find the report of application				The affiliated class-33 that belongs to	Pichia farinose (Lindner) Hansen pichia farinose
Former purposes-33	Do not find the report of application				ACCC2025	ACCC2026	AS2.86	AS2.87	AS2.705
AS2.803					ACCC2025	ACCC2026	AS2.86	AS2.87	AS2.705
AS2.803

					The affiliated class-34 that belongs to	Pichia membranaefaciens Hansen Pichia membranaefaciens
Former purposes-34	Do not find the report of application				The affiliated class-34 that belongs to	Pichia membranaefaciens Hansen Pichia membranaefaciens
Former purposes-34	Do not find the report of application				ACCC2027	AS2.89	AS2.661	AS2.1039
The affiliated class-35 that belongs to	Rhodosporidium toruloides Banno spore yeast of red winter				ACCC2027	AS2.89	AS2.661	AS2.1039
The affiliated class-35 that belongs to	Rhodosporidium toruloides Banno spore yeast of red winter				Former purposes-35	Do not find the report of application
ACCC2028					Former purposes-35	Do not find the report of application
ACCC2028					The affiliated class-35 that belongs to	Rhodotorula glutinis (Fresenius) Harrison rhodotorula
Former purposes-35	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc.				The affiliated class-35 that belongs to	Rhodotorula glutinis (Fresenius) Harrison rhodotorula
Former purposes-35					AS2.2029	AS2.280	ACCC2030	AS2.102	AS2.107
AS2.278	AS2.499	AS2.694	AS2.703	AS2.704	AS2.2029	AS2.280	ACCC2030	AS2.102	AS2.107
AS2.278	AS2.499	AS2.694	AS2.703	AS2.704	AS2.1146
The affiliated class-36 that belongs to	The little rhodotorula of Rhodotorula minuta (Saito) Harrison				AS2.1146
The affiliated class-36 that belongs to					Former purposes-36	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment etc.
AS2.277					Former purposes-36
AS2.277					The affiliated class-37 that belongs to	Rhodotorula rubar (Demme) Lodder rhodothece rubra
Former purposes-37	Fermenting carbohydrate, fermentation protein class, manufacturing food, condiment, feed etc.				The affiliated class-37 that belongs to	Rhodotorula rubar (Demme) Lodder rhodothece rubra
Former purposes-37					AS2.21	AS2.22	AS2.103	AS2.105	AS2.108
AS2.140	AS2.166	AS2.167	AS2.272	AS2.279	AS2.21	AS2.22	AS2.103	AS2.105	AS2.108
AS2.140	AS2.166	AS2.167	AS2.272	AS2.279	AS2.282	ACCC2031
The affiliated class-38 that belongs to	Saccharomyces carlsbergensis Hansen Ka Ersi yeast				AS2.282	ACCC2031
The affiliated class-38 that belongs to	Saccharomyces carlsbergensis Hansen Ka Ersi yeast				Former purposes-38	Make food, brew alcoholic beverages, feed etc.
AS2.113	ACCC2032	ACCC2033	AS2.312	AS2.116	Former purposes-38	Make food, brew alcoholic beverages, feed etc.
AS2.113	ACCC2032	ACCC2033	AS2.312	AS2.116	AS2.118	AS2.121	AS2.132	AS2.162	AS2.189
AS2.200	AS2.216	AS2.265	AS2.377	AS2.417	AS2.118	AS2.121	AS2.132	AS2.162	AS2.189
AS2.200	AS2.216	AS2.265	AS2.377	AS2.417	AS2.420	AS2.440	AS2.441	AS2.443	AS2.444
AS2.459	AS2.595	AS2.605	AS2.638	AS2.742	AS2.420	AS2.440	AS2.441	AS2.443	AS2.444
AS2.459	AS2.595	AS2.605	AS2.638	AS2.742	AS2.745	AS2.748	AS2.1042

The affiliated class-39 that belongs to	Saccharomyces uvarum Beijer saccharomyces uvarum
The affiliated class-39 that belongs to	Saccharomyces uvarum Beijer saccharomyces uvarum				Former purposes-39	Make food, brew alcoholic beverages, feed etc.
IFFI1023	IFFI1032	IFFI1036	IFFI1044	IFFI1072	Former purposes-39	Make food, brew alcoholic beverages, feed etc.
IFFI1023	IFFI1032	IFFI1036	IFFI1044	IFFI1072	IFFI1205	IFFI1207
The affiliated class-40 that belongs to	Saccharomyces willianus Saccardo Weir yeast				IFFI1205	IFFI1207
The affiliated class-40 that belongs to	Saccharomyces willianus Saccardo Weir yeast				Former purposes-40	Make food, brew alcoholic beverages, feed etc.
AS2.5	AS2.7	AS2.119	AS2.152	AS2.293	Former purposes-40	Make food, brew alcoholic beverages, feed etc.
AS2.5	AS2.7	AS2.119	AS2.152	AS2.293	AS2.381	AS2.392	AS2.434	AS2.614	AS2.1189
					AS2.381	AS2.392	AS2.434	AS2.614	AS2.1189
					The affiliated class-41 that belongs to	Saccharomyces sp. saccharomycete
Former purposes-41	Make brandy etc.				The affiliated class-41 that belongs to	Saccharomyces sp. saccharomycete
Former purposes-41	Make brandy etc.				AS2.311
The affiliated class-42 that belongs to	Saccharomycodes ludwigii Hansen rood class yeast				AS2.311
The affiliated class-42 that belongs to	Saccharomycodes ludwigii Hansen rood class yeast				Former purposes-42	Do not see the report of application
ACCC2044	AS2.243	AS2.508			Former purposes-42	Do not see the report of application
ACCC2044	AS2.243	AS2.508			The affiliated class-43 that belongs to	Saccharomycodes sinenses Yue China class yeast
Former purposes-43	Do not see the report of application				The affiliated class-43 that belongs to	Saccharomycodes sinenses Yue China class yeast
Former purposes-43	Do not see the report of application				AS2.1395
The affiliated class-44 that belongs to	Schizosaccharomyces octosporus Beijerinck eight spore fission yeasts				AS2.1395
The affiliated class-44 that belongs to					Former purposes-44	Do not see the report of application
ACCC2046	AS2.1148				Former purposes-44	Do not see the report of application
ACCC2046	AS2.1148				The affiliated class-45 that belongs to	Schizosaccharomyces pombe Lindner chestnut wine fission yeast
Former purposes-45	Lactose fermenters, brew alcoholic beverages, feed etc.				The affiliated class-45 that belongs to
Former purposes-45	Lactose fermenters, brew alcoholic beverages, feed etc.				ACCC2047	ACCC2048	AS2.214	AS2.248	AS2.249
AS2.255	AS2.257	AS2.259	AS2.260	AS2.274	ACCC2047	ACCC2048	AS2.214	AS2.248	AS2.249
AS2.255	AS2.257	AS2.259	AS2.260	AS2.274	AS2.994	AS2.1043	AS2.1149	AS2.1178	IFFI1056
The affiliated class-46 that belongs to	Sporobolomyces roseus Kluyver et van Niel shadow yeast				AS2.994	AS2.1043	AS2.1149	AS2.1178	IFFI1056
The affiliated class-46 that belongs to	Sporobolomyces roseus Kluyver et van Niel shadow yeast				Former purposes-46	Lactose fermenters, brew alcoholic beverages, feed, antibiotic etc.
ACCC2049	ACCC2050	AS2.19	AS2.962	AS2.1036	Former purposes-46
ACCC2049	ACCC2050	AS2.19	AS2.962	AS2.1036	ACCC2051	AS2.261	AS2.262
The affiliated class-47 that belongs to	Torulopsis Candida (Saito) Lodder Torulopsis candida				ACCC2051	AS2.261	AS2.262
The affiliated class-47 that belongs to	Torulopsis Candida (Saito) Lodder Torulopsis candida				Former purposes-47
AS2.270	ACCC2052				Former purposes-47
AS2.270	ACCC2052				The affiliated class-48 that belongs to	The unknown torulopsis of Torulopsis famta (Harrisn) Lodder et van Rij
Former purposes-48					The affiliated class-48 that belongs to
Former purposes-48					ACCC2053	AS2.685

The affiliated class-49 that belongs to	Torulopsis globosa (Olson et Hammer) Lodder et van Rij torulopsis
The affiliated class-49 that belongs to		Former purposes-49
ACCC2054	AS2.202	Former purposes-49
ACCC2054	AS2.202	The affiliated class-50 that belongs to	The usual torulopsis of Torulopsis inconspicua Lodder et Kreger van Rij
Former purposes-50		The affiliated class-50 that belongs to
Former purposes-50		AS2.75
		AS2.75
		The affiliated class-51 that belongs to	Trichosporon behrendii Lodder et Kreger van Rij shellfish thunder trichosporon cutaneum
Former purposes-51		The affiliated class-51 that belongs to
Former purposes-51		ACCC2056	AS2.1193
The affiliated class-52 that belongs to	Trichosporon capitatum Diddens et Lodder head trichosporon cutaneum	ACCC2056	AS2.1193
The affiliated class-52 that belongs to		Former purposes-52
ACCC2056	AS2.1385	Former purposes-52
ACCC2056	AS2.1385	The affiliated class-53 that belongs to	Trichosporon cutaneum (de Beurm et al.) Ota trichosporon cutaneum
Former purposes-53		The affiliated class-53 that belongs to
Former purposes-53		ACCC2057	AS2.25	AS2.570	AS2.571	AS2.1374
The affiliated class-54 that belongs to	Wickerhamia fluorescens (Soneda) Soneda Brunswick yeast	ACCC2057	AS2.25	AS2.570	AS2.571	AS2.1374
The affiliated class-54 that belongs to	Wickerhamia fluorescens (Soneda) Soneda Brunswick yeast	Former purposes-54
ACCC2058	AS2.1388	Former purposes-54

Subordinate list four

Specific yeast enlarges medium component table (in the 1000L nutrient solution)

Medium component	Quantity
Medium component	Quantity	Haw liquid	200L
Fruit of Chinese magnoliavine liquid	200L	Haw liquid	200L
Fruit of Chinese magnoliavine liquid	200L	Date liquid	200L
Soybean juice	200L	Date liquid	200L
Soybean juice	200L	Apple liquid	200L

Annotate: 1. in the table various liquid all be according to: the ratio of material/water=1/10 is processed into.

2. upper table nutrient solution will be adjusted in PH2.5 ± 0.2 scope.

Claims

1. a different frequency electronic signal that adopts simulation life ripple activates respectively common yeast recessive gene, makes it become different specific yeasts. The different albumen (enzyme) that the different recessive genes that these specific yeasts are activated are expressed, B cellular immunity gene, T cellular immunity gene, K cellular immunity gene and NK cellular immunity gene in narrow spectrum regulation and control, correction or the human activin, make under these immunocompromised or the immune response ability degradation gene recover normal, rebuild immune system. The present invention has been described in detail and has adopted these specific yeasts, has made biologic product. Improve immunity of organisms by taking this biologic product, reach the disease of preventing and treating kinds of tumors, uremia, various virus hepatitis, various neurological disorder and causing, lupus erythematosus, pulmonary tuberculosis, diabetes, various gastroenteritic ulcer, Various Diseases toxinfection etc.

2. as biologic product claimed in claim 1, it is characterized in that using yeast to make, related yeast is extensively as subordinate list three, but is not limited to subordinate list three.

3. as biologic product claimed in claim 1, it is characterized in that employed raw material is that fructus crataegi cuneatae (or Malus baccata), wild jujube, the fruit of Chinese magnoliavine, ginseng, soybean etc. are made except yeast.

4. as biologic product claimed in claim 1, it is characterized in that using the electronic signal of the different frequency of simulation life ripple to activate common yeast.

5. as biologic product claimed in claim 1, it is characterized in that making the immunomodulator of liquid and solid, and be used for the reconstruction of immune function of human body, the regulation and control of immunogene etc.